MD08G1143200.v1.1

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009179
Physical Location & Seq
Forward (+)
14224720 .. 14229820
5101 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1143200.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGAGAGGGGTAGCTTTTTTGTTGTCGGTGCTGTTGTTTGCCACTTGCCACTCAGCCTTCTCCCTCATCGATGGTTACTTAGAAAATGGCAATTTTGAGCTACCCCCTAAGCCATCAGACCTCAAGGGTTCCGAAGTGATTGGCCGCTATGCCATACCAAAGTGGGAGATTTCTGGGTTTGTGGAGTACATTAAGTCAGGCCAAAAGCAAGGGGACATGTTGCTGGTTGTCCCTGAGGGGGCCTATGCAGTTAGGCTCGGAAATGAGGCATTGATTAAGCAGAGCATTAAAGTGATTAAGGGATTGTACTACTCAATCACTTTTAGTGCAGCCAGAACTTGTGCTCAAGAGGAACGATTGAACATTTCCGTGGCACCGGACTCTGGAGTGCTGCCTATCCAAACAGTGTATAGCAGCAATGGTTGGGATTCATATGCTTGGGCGTTTCAGGCGGATTATGAGACAATTGAACTTGTTTTGCATAACCCCGGCGTCGAGGAGGATCCGGCTTGCGGGCCTCTAATCGATTCGGTCGCCTTCCGGACTTTGTACCCTCCTCGAGCAACCAACAAGAACTTGATCAAAAACGCCGGATTTGAAGAAGGTCCATACGTGTTCCCAAACACATCTTGGGGAGTCCTAATCCCACCAAACATAGAAGATGACCACTCTCCATTACCAGGTTGGATGGTGGAGTCCCTCAAAGCAGTGAAGTACATAGACTCAGACCACTTCTCAGTCCCGGAAGGAAAAAGAGCGGTGGAACTTGTGGCCGGAAAAGAAAGCGCAATCGCCCAAGTTGTCCGAACCATCCCTGGAAAAACCTATGTGCTCTCGTTCTCAGTAGGAGATGCAAGCAACTCATGCGAAGGCTCCTTGATCGTGGAGGCATTTGCAGGGAAGAACACTGTGAAAGTGCCTTATGAGTCCAAAGGCAAAGGCGGGTTTAAGCGGGCTGTGCTCAAATTCGTGGCGGTTTCGACCAGGACACGTGTCATGTTTCTAAGCACATATTACACCATGAGGAGTGATGATTTCTCTTCTCTGTGTGGACCTGTGCTTGATGATGTGAAGCTGTTGAGCCTTCGGAAACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

366

Amino Acids

39.95

Weight (kDa)

5.93

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF642 PF04862 25 - 180 9.2e-71 Protein of unknown function (DUF642)
DUF642 PF04862 193 - 359 7e-16 Protein of unknown function (DUF642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32460 AT4G32460 AT4G32460 AT4G32460 AT5G11420 AT5G25460
fragaria_vesca FvH4_2g29680
malus_domestica MD08G1143200.v1.1 MD15G1120000.v1.1
prunus_persica Prupe.1G472800_v2.0.a1
pyrus_communis pycom08g12190 pycom15g10810
rosa_chinensis RchiOBHm_Chr6g0298661
rosa_laevigata RLG00000011495
rosa_multiflora Rmu_sc0008099.1_g000008
rosa_roxburghii Rroxscaffold_7G00169440
rosa_rugosa Rorug06G0281900 Rorug06G0282000
rosa_samantha Rh6AG393500 Rh6BG401300 Rh6DG393900
rosa_wichuraiana Rw6G034410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 373
AccBSI CCGCTC 1 cut(s) 758
AccIII TCCGGA 1 cut(s) 540
AciI CCGC 7 cut(s) 145, 452, 513, 758, 942, 952, 974
AclWI GGATC 2 cut(s) 497, 510
AcoI YGGCCR 2 cut(s) 142, 771
AcsI RAATTY 1 cut(s) 965
AcvI CACGTG 1 cut(s) 992
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 4 cut(s) 188, 308, 551, 716
AfiI CCNNNNNNNGG 4 cut(s) 238, 383, 512, 680
AflIII ACRYGT 4 cut(s) 216, 612, 989, 991
AgsI TTSAA 3 cut(s) 361, 470, 599
AjnI CCWGG 3 cut(s) 679, 814, 983
AluBI AGCT 3 cut(s) 14, 100, 1075
AluI AGCT 3 cut(s) 14, 100, 1075
Alw21I GWGCWC 3 cut(s) 346, 834, 963
Alw26I GTCTC 1 cut(s) 455
AlwI GGATC 2 cut(s) 497, 510
Ama87I CYCGRG 1 cut(s) 558
Aor13HI TCCGGA 1 cut(s) 540
AoxI GGCC 5 cut(s) 142, 199, 240, 515, 771
ApeKI GCWGC 3 cut(s) 329, 391, 414
ApoI RAATTY 1 cut(s) 965
AspLEI GCGC 1 cut(s) 788
AspS9I GGNCC 4 cut(s) 240, 515, 605, 1052
AsuC2I CCSGG 2 cut(s) 489, 743
AvaI CYCGRG 1 cut(s) 558
AvaII GGWCC 2 cut(s) 605, 1052
AxyI CCTNAGG 1 cut(s) 234
BamHI GGATCC 1 cut(s) 502
BanI GGYRCC 1 cut(s) 373
BbrPI CACGTG 1 cut(s) 992
Bbv12I GWGCWC 3 cut(s) 346, 834, 963
BbvI GCAGC 3 cut(s) 341, 378, 426
BccI CCATC 4 cut(s) 65, 121, 682, 818
BciT130I CCWGG 3 cut(s) 681, 816, 985
BclI TGATCA 1 cut(s) 579
BcnI CCSGG 2 cut(s) 489, 743
BcoDI GTCTC 1 cut(s) 455
BfaI CTAG 1 cut(s) 1096
BisI GCNGC 4 cut(s) 145, 330, 392, 415
BlsI GCNGC 4 cut(s) 146, 331, 393, 416
Bme1390I CCNGG 5 cut(s) 489, 681, 743, 816, 985
Bme18I GGWCC 2 cut(s) 605, 1052
BmeT110I CYCGRG 1 cut(s) 558
BmgT120I GGNCC 4 cut(s) 240, 515, 605, 1052
BmiI GGNNCC 5 cut(s) 130, 241, 375, 504, 874
BmrFI CCNGG 5 cut(s) 489, 681, 743, 816, 985
BmsI GCATC 1 cut(s) 841
BoxI GACNNNNGTC 1 cut(s) 992
BpmI CTGGAG 1 cut(s) 405
Bpu10I CCTNAGC 1 cut(s) 108
BpuEI CTTGAG 2 cut(s) 107, 330
BpuMI CCSGG 2 cut(s) 489, 743
Bsa29I ATCGAT 2 cut(s) 69, 525
BsaAI YACGTR 2 cut(s) 613, 992
BsaHI GRCGYC 1 cut(s) 492
BsaJI CCNNGG 3 cut(s) 369, 487, 814
BsaWI WCCGGW 2 cut(s) 376, 540
Bsc4I CCNNNNNNNGG 4 cut(s) 238, 383, 512, 680
Bse21I CCTNAGG 1 cut(s) 234
Bse3DI GCAATG 1 cut(s) 424
BseAI TCCGGA 1 cut(s) 540
BseBI CCWGG 3 cut(s) 681, 816, 985
BseCI ATCGAT 2 cut(s) 69, 525
BseDI CCNNGG 3 cut(s) 369, 487, 814
BseGI GGATG 2 cut(s) 693, 810
BseLI CCNNNNNNNGG 4 cut(s) 238, 383, 512, 680
BseMI GCAATG 1 cut(s) 424
BseMII CTCAG 5 cut(s) 66, 225, 738, 750, 855
BseRI GAGGAG 3 cut(s) 512, 546, 1039
BseXI GCAGC 3 cut(s) 341, 378, 426
BsgI GTGCAG 1 cut(s) 348
Bsh1285I CGRYCG 1 cut(s) 534
BshFI GGCC 5 cut(s) 144, 201, 242, 517, 773
BshNI GGYRCC 1 cut(s) 373
BshVI ATCGAT 2 cut(s) 69, 525
BsiEI CGRYCG 1 cut(s) 534
BsiHKAI GWGCWC 3 cut(s) 346, 834, 963
BsiHKCI CYCGRG 1 cut(s) 558
BsiSI CCGG 7 cut(s) 377, 489, 506, 541, 591, 743, 774
BslFI GGGAC 4 cut(s) 215, 227, 682, 725
BslI CCNNNNNNNGG 4 cut(s) 238, 383, 512, 680
BsmAI GTCTC 1 cut(s) 455
BsmFI GGGAC 4 cut(s) 215, 227, 682, 725
BsnI GGCC 5 cut(s) 144, 201, 242, 517, 773
BsoBI CYCGRG 1 cut(s) 558
Bsp1286I GDGCHC 3 cut(s) 346, 834, 963
Bsp13I TCCGGA 1 cut(s) 540
Bsp143I GATC 3 cut(s) 502, 579, 879
BspACI CCGC 7 cut(s) 145, 452, 513, 758, 942, 952, 974
BspANI GGCC 5 cut(s) 144, 201, 242, 517, 773
BspCNI CTCAG 5 cut(s) 65, 226, 737, 749, 854
BspDI ATCGAT 2 cut(s) 69, 525
BspEI TCCGGA 1 cut(s) 540
BspLI GGNNCC 5 cut(s) 130, 241, 375, 504, 874
BspPI GGATC 2 cut(s) 497, 510
BspT107I GGYRCC 1 cut(s) 373
BsrBI CCGCTC 1 cut(s) 758
BsrDI GCAATG 1 cut(s) 424
BssECI CCNNGG 3 cut(s) 369, 487, 814
BssMI GATC 3 cut(s) 502, 579, 879
BssNI GRCGYC 1 cut(s) 492
Bst2UI CCWGG 3 cut(s) 681, 816, 985
Bst4CI ACNGT 2 cut(s) 406, 910
Bst6I CTCTTC 1 cut(s) 1045
BstACI GRCGYC 1 cut(s) 492
BstAPI GCANNNNNTGC 1 cut(s) 864
BstBAI YACGTR 2 cut(s) 613, 992
BstC8I GCNNGC 4 cut(s) 511, 515, 856, 954
BstDEI CTNAG 8 cut(s) 52, 79, 108, 234, 724, 736, 841, 1004
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 2 cut(s) 693, 810
BstHHI GCGC 1 cut(s) 788
BstKTI GATC 3 cut(s) 505, 582, 882
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 3 cut(s) 502, 579, 879
BstMCI CGRYCG 1 cut(s) 534
BstMWI GCNNNNNNNGC 3 cut(s) 449, 864, 958
BstNI CCWGG 3 cut(s) 681, 816, 985
BstNSI RCATGY 1 cut(s) 220
BstPAI GACNNNNGTC 1 cut(s) 992
BstSCI CCNGG 5 cut(s) 487, 679, 741, 814, 983
BstV1I GCAGC 3 cut(s) 341, 378, 426
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
Bsu15I ATCGAT 2 cut(s) 69, 525
Bsu36I CCTNAGG 1 cut(s) 234
BsuRI GGCC 5 cut(s) 144, 201, 242, 517, 773
BsuTUI ATCGAT 2 cut(s) 69, 525
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 2 cut(s) 693, 810
BtsI GCAGTG 1 cut(s) 714
BtsIMutI CAGTG 3 cut(s) 411, 714, 906
Cac8I GCNNGC 4 cut(s) 511, 515, 856, 954
CfoI GCGC 1 cut(s) 788
Cfr13I GGNCC 4 cut(s) 240, 515, 605, 1052
ClaI ATCGAT 2 cut(s) 69, 525
CseI GACGC 1 cut(s) 481
CsiI ACCWGGT 1 cut(s) 679
Csp6I GTAC 4 cut(s) 187, 307, 550, 715
CviAII CATG 4 cut(s) 217, 864, 997, 1021
CviQI GTAC 4 cut(s) 187, 307, 550, 715
DdeI CTNAG 8 cut(s) 52, 79, 108, 234, 724, 736, 841, 1004
DpnI GATC 3 cut(s) 504, 581, 881
DpnII GATC 3 cut(s) 502, 579, 879
EaeI YGGCCR 2 cut(s) 142, 771
Eam1104I CTCTTC 1 cut(s) 1045
EarI CTCTTC 1 cut(s) 1045
EciI GGCGGA 1 cut(s) 467
Eco47I GGWCC 2 cut(s) 605, 1052
Eco72I CACGTG 1 cut(s) 992
Eco81I CCTNAGG 1 cut(s) 234
Eco88I CYCGRG 1 cut(s) 558
EcoO109I RGGNCCY 1 cut(s) 240
EcoRII CCWGG 3 cut(s) 679, 814, 983
FaeI CATG 4 cut(s) 220, 867, 1000, 1024
FaqI GGGAC 4 cut(s) 215, 227, 682, 725
FatI CATG 4 cut(s) 216, 863, 996, 1020
FauI CCCGC 3 cut(s) 506, 935, 945
FauNDI CATATG 1 cut(s) 433
FbaI TGATCA 1 cut(s) 579
Fnu4HI GCNGC 4 cut(s) 145, 330, 392, 415
FokI GGATG 2 cut(s) 700, 797
Fsp4HI GCNGC 4 cut(s) 145, 330, 392, 415
FspBI CTAG 1 cut(s) 1096
GlaI GCGC 1 cut(s) 787
GluI GCNGC 4 cut(s) 145, 330, 392, 415
GsuI CTGGAG 1 cut(s) 405
HaeIII GGCC 5 cut(s) 144, 201, 242, 517, 773
HapII CCGG 7 cut(s) 377, 489, 506, 541, 591, 743, 774
HgaI GACGC 1 cut(s) 481
HhaI GCGC 1 cut(s) 788
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 4 cut(s) 220, 867, 1000, 1024
Hin6I GCGC 1 cut(s) 786
HinP1I GCGC 1 cut(s) 786
HinfI GANTC 7 cut(s) 380, 428, 527, 636, 695, 722, 926
HpaII CCGG 7 cut(s) 377, 489, 506, 541, 591, 743, 774
Hpy166II GTNNAC 1 cut(s) 1052
Hpy188I TCNGA 6 cut(s) 118, 133, 260, 727, 806, 1089
Hpy188III TCNNGA 3 cut(s) 347, 384, 541
Hpy8I GTNNAC 1 cut(s) 1052
Hpy99I CGWCG 1 cut(s) 497
HpyAV CCTTC 6 cut(s) 67, 547, 596, 740, 863, 1094
HpyCH4III ACNGT 2 cut(s) 406, 910
HpyCH4IV ACGT 2 cut(s) 612, 991
HpyCH4V TGCA 5 cut(s) 248, 329, 481, 854, 896
HpyF10VI GCNNNNNNNGC 3 cut(s) 449, 864, 958
HpyF3I CTNAG 8 cut(s) 52, 79, 108, 234, 724, 736, 841, 1004
HpySE526I ACGT 2 cut(s) 612, 991
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 4 cut(s) 220, 867, 1000, 1024
HspAI GCGC 1 cut(s) 786
Kpn2I TCCGGA 1 cut(s) 540
Ksp22I TGATCA 1 cut(s) 579
Kzo9I GATC 3 cut(s) 502, 579, 879
LmnI GCTCC 1 cut(s) 878
Lsp1109I GCAGC 3 cut(s) 341, 378, 426
LweI GCATC 1 cut(s) 841
MabI ACCWGGT 1 cut(s) 679
MaeI CTAG 1 cut(s) 1096
MaeII ACGT 2 cut(s) 612, 991
MaeIII GTNAC 1 cut(s) 74
MalI GATC 3 cut(s) 504, 581, 881
MbiI CCGCTC 1 cut(s) 758
MboI GATC 3 cut(s) 502, 579, 879
MboII GAAGA 4 cut(s) 611, 671, 913, 1032
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 3 cut(s) 346, 834, 963
MluCI AATT 3 cut(s) 91, 465, 965
MlyI GAGTC 5 cut(s) 374, 645, 704, 716, 935
MmeI TCCRAC 1 cut(s) 665
MroI TCCGGA 1 cut(s) 540
MseI TTAA 5 cut(s) 192, 276, 288, 297, 948
MslI CAYNNNNRTG 2 cut(s) 290, 368
MspI CCGG 7 cut(s) 377, 489, 506, 541, 591, 743, 774
MspR9I CCNGG 5 cut(s) 489, 681, 743, 816, 985
MunI CAATTG 1 cut(s) 465
MvaI CCWGG 3 cut(s) 681, 816, 985
MwoI GCNNNNNNNGC 3 cut(s) 449, 864, 958
NciI CCSGG 2 cut(s) 489, 743
NdeI CATATG 1 cut(s) 433
NdeII GATC 3 cut(s) 502, 579, 879
NlaIII CATG 4 cut(s) 220, 867, 1000, 1024
NlaIV GGNNCC 5 cut(s) 130, 241, 375, 504, 874
NspI RCATGY 1 cut(s) 220
PaeR7I CTCGAG 1 cut(s) 558
PciI ACATGT 1 cut(s) 216
PfeI GAWTC 2 cut(s) 428, 527
PfoI TCCNGGA 1 cut(s) 741
PkrI GCNGC 4 cut(s) 146, 331, 393, 416
PleI GAGTC 5 cut(s) 374, 644, 703, 716, 934
PmaCI CACGTG 1 cut(s) 992
PmlI CACGTG 1 cut(s) 992
PpsI GAGTC 5 cut(s) 374, 644, 703, 716, 934
Ppu21I YACGTR 2 cut(s) 613, 992
PscI ACATGT 1 cut(s) 216
PshAI GACNNNNGTC 1 cut(s) 992
Psp6I CCWGG 3 cut(s) 679, 814, 983
PspCI CACGTG 1 cut(s) 992
PspGI CCWGG 3 cut(s) 679, 814, 983
PspN4I GGNNCC 5 cut(s) 130, 241, 375, 504, 874
PspPI GGNCC 4 cut(s) 240, 515, 605, 1052
PspXI VCTCGAGB 1 cut(s) 558
PsuI RGATCY 1 cut(s) 502
RsaI GTAC 4 cut(s) 188, 308, 551, 716
RsaNI GTAC 4 cut(s) 187, 307, 550, 715
RseI CAYNNNNRTG 2 cut(s) 290, 368
SaqAI TTAA 5 cut(s) 192, 276, 288, 297, 948
SatI GCNGC 4 cut(s) 145, 330, 392, 415
Sau3AI GATC 3 cut(s) 502, 579, 879
Sau96I GGNCC 4 cut(s) 240, 515, 605, 1052
SchI GAGTC 5 cut(s) 374, 645, 704, 716, 935
ScrFI CCNGG 5 cut(s) 489, 681, 743, 816, 985
SduI GDGCHC 3 cut(s) 346, 834, 963
SexAI ACCWGGT 1 cut(s) 679
SfaNI GCATC 1 cut(s) 841
Sfr274I CTCGAG 1 cut(s) 558
SinI GGWCC 2 cut(s) 605, 1052
SlaI CTCGAG 1 cut(s) 558
SmiMI CAYNNNNRTG 2 cut(s) 290, 368
SmlI CTYRAG 3 cut(s) 122, 345, 558
SmoI CTYRAG 3 cut(s) 122, 345, 558
Sse9I AATT 3 cut(s) 91, 465, 965
SsiI CCGC 7 cut(s) 145, 452, 513, 758, 942, 952, 974
SspMI CTAG 1 cut(s) 1096
StyD4I CCNGG 5 cut(s) 487, 679, 741, 814, 983
TaaI ACNGT 2 cut(s) 406, 910
TaiI ACGT 2 cut(s) 615, 994
TaqI TCGA 5 cut(s) 69, 495, 525, 559, 980
TaqII GACCGA 1 cut(s) 520
TasI AATT 3 cut(s) 91, 465, 965
TatI WGTACW 3 cut(s) 186, 306, 714
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 2 cut(s) 428, 527
Tru1I TTAA 5 cut(s) 192, 276, 288, 297, 948
Tru9I TTAA 5 cut(s) 192, 276, 288, 297, 948
TscAI CASTG 3 cut(s) 411, 714, 913
TseI GCWGC 3 cut(s) 329, 391, 414
TspDTI ATGAA 1 cut(s) 420
TspGWI ACGGA 1 cut(s) 358
TspRI CASTG 3 cut(s) 411, 714, 913
VpaK11BI GGWCC 2 cut(s) 605, 1052
XapI RAATTY 1 cut(s) 965
XceI RCATGY 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 2 cut(s) 627, 681
XhoI CTCGAG 1 cut(s) 558
XspI CTAG 1 cut(s) 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.