AT5G27270

Pentacotripeptide-repeat region of PRORP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009191
Physical Location & Seq
Forward (+)
9605484 .. 9609804
4321 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27270.3

Sequence Viewer

Length: 3117 bp
ATGAAGAGTGACTTCCTCACTTCAACAACACACTTCAATCCTTCAATATTCCTTCCCAAAATACCCTCAAGAAACTCTAGGATCTCAATAAAGTCATCATCTTCTTCTTCCAAAGTCCGACCCGACCCGTGGTCACTCAGCGACGGTAACCCGGAAAAACCAAAACCGAGATACGAGAGACCGAAGCATCCATTATCAGACGACGACGCTCGACGAATCATAAAGAAGAAAGCACAATACTTAAGCACGTTAAGAAGGAACCAAGGCTCACAAGCAATGACTCCCAAATGGATAAAGCGAACTCCAGAGCAAATGGTTCAGTATTTGGAAGATGATCGGAATGGTCAGATGTATGGGAAACATGTGGTGGCTGCGATTAAGACGGTGAGAGGTTTGTCACAGAGAAGACAAGGTAGTGATGATATGAGATTTGTGATGAGTAGCTTTGTAGCGAAGTTGAGTTTTAGAGATATGTGTGTTGTTTTGAAAGAACAGAGAGGTTGGAGACAAGTTAGAGACTTTTTCTCTTGGATGAAATTGCAGTTGAGTTATCGTCCGAGTGTGGTTGTGTATACGATTGTTCTTCGTTTATATGGACAAGTTGGGAAGATAAAGATGGCTGAGGAGACTTTTTTAGAAATGCTTGAAGTTGGATGTGAACCAGATGCTGTAGCTTGTGGTACAATGTTGTGTACTTATGCTAGATGGGGACGTCATAGTGCTATGTTGACGTTTTACAAAGCTGTTCAAGAAAGGCGGATACTTTTGTCGACTTCTGTGTATAATTTCATGCTGTCGTCTCTTCAGAAGAAATCTTTTCATGGTAAAGTGATAGATTTGTGGTTAGAAATGGTGGAGGAAGGTGTGCCTCCGAATGAATTCACGTATACGTTAGTTGTGAGCTCTTATGCTAAACAGGGTTTTAAGGAGGAGGCGTTGAAGGCTTTTGGTGAGATGAAGAGTTTGGGGTTTGTCCCCGAGGAAGTGACTTACAGCTCGGTTATTAGTTTAAGTGTGAAGGCTGGTGATTGGGAGAAGGCTATTGGATTGTATGAGGATATGAGATCTCAGGGAATCGTTCCGAGTAACTATACTTGTGCTACTATGTTAAGTTTATATTACAAAACGGAGAACTACCCGAAAGCGCTCTCTCTCTTTGCAGACATGGAAAGAAATAAGATTCCAGCTGATGAGGTCATCCGCGGGTTGATCATCAGAATATATGGGAAGCTCGGACTTTTCCATGATGCACAGAGTATGTTTGAAGAGACTGAACGCCTGAATTTACTGGCTGATGAGAAAACATATCTGGCAATGTCTCAAGTTCATTTGAATTCGGGAAATGTTGTCAAAGCATTAGATGTAATTGAAATGATGAAGACAAGAGATATTCCTCTTTCCAGATTTGCCTATATTGTCATGTTACAGTGTTATGCTAAGATACAGAATGTTGACTGTGCTGAGGAAGCATTTCGTGCTTTATCGAAGACTGGACTTCCTGATGCCAGTTCTTGTAACGACATGCTTAATTTGTATACCAGACTCAATTTAGGAGAGAAAGCTAAGGGTTTTATCAAGCAGATAATGGTTGATCAAGTGCACTTTGATATAGAGCTTTACAAGACGGCTATGAGAGTGTATTGCAAGGAAGGAATGGTAGCAGAAGCTCAAGATTTGATAGTAAAAATGGGTAGAGAGGCAAGGGTTAAGGATAACAGATTTGTTCAGACACTTGCAGAGTCTATGCATATCGTTAACAAGCACGATAAACATGAAGCAGTTTTAAACGTGAGTCAACTTGATGTCATGGCTCTTGGACTGATGCTCAATTTGCGACTGAAAGAGGGAAATTTGAATGAGACTAAAGCAATCTTGAACTTAATGTTCAAGACTGATCTTGGTTCATCGGCAGTAAATCGAGTCATAAGTAGCTTTGTGAGAGAAGGTGATGTATCTAAAGCAGAAATGATTGCCGATATAATTATCAGACTAGGACTCAGGATGGAGGAGGAAACAATCGCGACTTTGATTGCTGTATATGGGAGGCAACATAAGCTTAAAGAAGCGAAACGGCTTTACCTTGCAGCAGGAGAATCTAAAACTCCGGGGAAATCTGTTATCCGCTCAATGATTGATGCTTATGTCAGATGTGGCTGGCTTGAAGATGCATATGGACTCTTTATGGAATCAGCAGAAAAGGGCTGTGACCCAGGAGCTGTTACCATTAGCATACTTGTGAATGCTCTAACAAATCGAGGGAAACACCGAGAAGCAGAACATATATCACGGACCTGCCTTGAAAAAAACATAGAGCTTGACACCGTTGGCTATAATACTCTTATCAAGGCAATGCTAGAAGCAGGTAAACTACAATGTGCATCTGAAATTTATGAGCGGATGCATACCTCAGGTGTTCCTTGTTCGATTCAGACCTACAACACAATGATCAGTGTATATGGGCGAGGTCTGCAGCTGGACAAAGCAATTGAGATTTTTAGTAACGCTCGCAGATCAGGGTTGTATCTGGACGAGAAAATTTACACAAACATGATTATGCATTATGGCAAGGGGGGAAAGATGAGTGAAGCATTGTCTTTGTTTAGTGAAATGCAGAAGAAAGGGATTAAACCTGGAACGCCTAGTTACAACATGATGGTCAAAATATGTGCAACCAGTAGACTCCATCACGAGGTTGATGAACTTTTGCAAGCAATGGAGAGAAATGGTCGCTGTACTGACTTATCCACATACCTCACCCTGATTCAAGTCTATGCTGAGAGTTCACAGTTTGCAGAAGCAGAGAAAACGATAACTCTTGTTAAAGAGAAAGGCATCCCGCTATCTCACAGCCATTTCAGTTCATTGCTCTCTGCTCTTGTCAAAGCTGGGATGATGGAGGAGGCTGAAAGGACATACTGCAAGATGTCTGAAGCCGGTATAAGTCCAGACAGTGCTTGCAAGCGGACGATCCTAAAGGGATATATGACTTGTGGTGACGCAGAGAAAGGAATCTTGTTCTATGAGAAAATGATAAGAAGCTCTGTGGAAGATGACAGATTTGTTAGCAGTGTAGTTGAAGATCTGTATAAAGCTGTTGGTAAAGAACAAGATGTATAA

Protein Analysis

1038

Amino Acids

117.45

Weight (kDa)

8.78

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 35 - 167 1.3e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 53 - 100 7.8e-08 PPR repeat family
PPR_3 PF13812 78 - 134 8e-06 Pentatricopeptide repeat domain
PPR PF01535 89 - 119 6.1e-06 PPR repeat
PPR_2 PF13041 124 - 166 2.7e-09 PPR repeat family
PPR PF01535 124 - 154 2.9e-06 PPR repeat
PPR_2 PF13041 504 - 545 6e-06 PPR repeat family
PPR_3 PF13812 556 - 615 3.7e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 568 - 614 1.6e-10 PPR repeat family
PPR_long PF17177 584 - 678 9.7e-06 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 623 - 729 2.6e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 626 - 684 2e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 640 - 685 2.8e-11 PPR repeat family
PPR PF01535 641 - 670 1.5e-07 PPR repeat
PPR_long PF17177 653 - 811 1.9e-09 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 730 - 787 8.8e-07 Fungal tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 715
Acc36I ACCTGC 2 cut(s) 2300, 2351
AccBSI CCGCTC 2 cut(s) 2124, 2395
AccI GTMKAC 5 cut(s) 572, 770, 887, 1535, 2677
AccII CGCG 2 cut(s) 1203, 2021
AciI CCGC 7 cut(s) 757, 1201, 1203, 2122, 2395, 2837, 2962
AclWI GGATC 2 cut(s) 89, 2962
AcsI RAATTY 6 cut(s) 878, 1282, 1333, 1849, 2385, 2535
AcuI CTGAAG 2 cut(s) 788, 2949
AcyI GRCGYC 1 cut(s) 712
AfaI GTAC 3 cut(s) 682, 694, 2734
AfeI AGCGCT 1 cut(s) 1146
AfiI CCNNNNNNNGG 2 cut(s) 129, 2689
AflII CTTAAG 1 cut(s) 241
AflIII ACRYGT 1 cut(s) 361
AhdI GACNNNNNGTC 1 cut(s) 130
AjnI CCWGG 2 cut(s) 2209, 2629
Alw21I GWGCWC 2 cut(s) 905, 1602
Alw26I GTCTC 8 cut(s) 172, 499, 510, 620, 804, 1262, 1323, 1853
Alw44I GTGCAC 1 cut(s) 1598
AlwI GGATC 2 cut(s) 89, 2962
AlwNI CAGNNNCTG 2 cut(s) 668, 2216
Ama87I CYCGRG 1 cut(s) 977
Aor51HI AGCGCT 1 cut(s) 1146
ApaLI GTGCAC 1 cut(s) 1598
ApeKI GCWGC 3 cut(s) 371, 2084, 2470
ApoI RAATTY 6 cut(s) 878, 1282, 1333, 1849, 2385, 2535
Asp700I GAANNNNTTC 2 cut(s) 878, 1470
AspLEI GCGC 1 cut(s) 1147
AspS9I GGNCC 1 cut(s) 2289
AsuC2I CCSGG 2 cut(s) 152, 2106
AsuHPI GGTGA 6 cut(s) 397, 962, 1037, 1958, 2746, 3005
AvaI CYCGRG 1 cut(s) 977
AvaII GGWCC 1 cut(s) 2289
AxyI CCTNAGG 1 cut(s) 2407
BaeGI GKGCMC 1 cut(s) 1602
BaeI ACNNNNGTAYC 2 cut(s) 752, 785
BanII GRGCYC 1 cut(s) 905
BauI CACGAG 1 cut(s) 2687
BbsI GAAGAC 3 cut(s) 412, 1385, 1493
Bbv12I GWGCWC 2 cut(s) 905, 1602
BbvCI CCTCAGC 2 cut(s) 621, 1461
BbvI GCAGC 3 cut(s) 358, 2096, 2482
BccI CCATC 6 cut(s) 610, 699, 1996, 2647, 2691, 2887
BceAI ACGGC 2 cut(s) 1641, 2087
BciT130I CCWGG 2 cut(s) 2211, 2631
BciVI GTATCC 1 cut(s) 753
BclI TGATCA 3 cut(s) 1209, 1591, 2445
BcnI CCSGG 2 cut(s) 152, 2106
BcoDI GTCTC 8 cut(s) 172, 499, 510, 620, 804, 1262, 1323, 1853
BfaI CTAG 5 cut(s) 78, 702, 1991, 2354, 2640
BfmI CTRYAG 2 cut(s) 669, 2468
BfoI RGCGCY 1 cut(s) 1148
BfrI CTTAAG 1 cut(s) 241
BfuAI ACCTGC 2 cut(s) 2300, 2351
BfuI GTATCC 1 cut(s) 753
BglII AGATCT 2 cut(s) 1064, 3079
BisI GCNGC 3 cut(s) 372, 2085, 2471
BlsI GCNGC 3 cut(s) 373, 2086, 2472
Bme1390I CCNGG 4 cut(s) 152, 2106, 2211, 2631
Bme18I GGWCC 1 cut(s) 2289
BmeRI GACNNNNNGTC 1 cut(s) 130
BmeT110I CYCGRG 1 cut(s) 977
BmgT120I GGNCC 1 cut(s) 2289
BmiI GGNNCC 1 cut(s) 260
BmrFI CCNGG 4 cut(s) 152, 2106, 2211, 2631
BpiI GAAGAC 3 cut(s) 412, 1385, 1493
BpmI CTGGAG 1 cut(s) 288
Bpu10I CCTNAGC 3 cut(s) 621, 1461, 1563
BpuEI CTTGAG 3 cut(s) 52, 1305, 1653
BpuMI CCSGG 2 cut(s) 152, 2106
BsaAI YACGTR 1 cut(s) 885
BsaHI GRCGYC 1 cut(s) 712
BsaI GGTCTC 1 cut(s) 172
BsaJI CCNNGG 6 cut(s) 128, 262, 978, 1201, 2105, 2209
BsaXI ACNNNNNCTCC 2 cut(s) 920, 950
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 2689
Bse118I RCCGGY 1 cut(s) 2933
Bse1I ACTGG 4 cut(s) 1293, 1495, 1506, 2673
Bse21I CCTNAGG 1 cut(s) 2407
Bse3DI GCAATG 5 cut(s) 282, 1320, 2355, 2718, 2861
BseBI CCWGG 2 cut(s) 2211, 2631
BseDI CCNNGG 6 cut(s) 128, 262, 978, 1201, 2105, 2209
BseGI GGATG 8 cut(s) 187, 537, 659, 1197, 2007, 2403, 2832, 2895
BseLI CCNNNNNNNGG 2 cut(s) 129, 2689
BseMI GCAATG 5 cut(s) 282, 1320, 2355, 2718, 2861
BseMII CTCAG 7 cut(s) 151, 612, 1082, 1452, 2011, 2421, 2766
BseNI ACTGG 4 cut(s) 1293, 1495, 1506, 2673
BseRI GAGGAG 4 cut(s) 638, 944, 2021, 2912
BseSI GKGCMC 1 cut(s) 1602
BseXI GCAGC 3 cut(s) 358, 2096, 2482
BseYI CCCAGC 1 cut(s) 2885
Bsh1236I CGCG 2 cut(s) 1203, 2021
BsiHKAI GWGCWC 2 cut(s) 905, 1602
BsiHKCI CYCGRG 1 cut(s) 977
BsiSI CCGG 3 cut(s) 152, 2105, 2934
BslFI GGGAC 2 cut(s) 723, 959
BslI CCNNNNNNNGG 2 cut(s) 129, 2689
BsmAI GTCTC 8 cut(s) 172, 499, 510, 620, 804, 1262, 1323, 1853
BsmBI CGTCTC 1 cut(s) 804
BsmFI GGGAC 2 cut(s) 723, 959
BsmI GAATGC 1 cut(s) 2245
Bso31I GGTCTC 1 cut(s) 172
BsoBI CYCGRG 1 cut(s) 977
Bsp1286I GDGCHC 2 cut(s) 905, 1602
Bsp68I TCGCGA 1 cut(s) 2021
BspACI CCGC 7 cut(s) 757, 1201, 1203, 2122, 2395, 2837, 2962
BspCNI CTCAG 7 cut(s) 150, 613, 1081, 1453, 2010, 2420, 2767
BspFNI CGCG 2 cut(s) 1203, 2021
BspLI GGNNCC 1 cut(s) 260
BspMAI CTGCAG 1 cut(s) 2472
BspMI ACCTGC 2 cut(s) 2300, 2351
BspPI GGATC 2 cut(s) 89, 2962
BspTI CTTAAG 1 cut(s) 241
BspTNI GGTCTC 1 cut(s) 172
BsrBI CCGCTC 2 cut(s) 2124, 2395
BsrDI GCAATG 5 cut(s) 282, 1320, 2355, 2718, 2861
BsrFI RCCGGY 1 cut(s) 2933
BsrI ACTGG 4 cut(s) 1293, 1495, 1506, 2673
BssAI RCCGGY 1 cut(s) 2933
BssECI CCNNGG 6 cut(s) 128, 262, 978, 1201, 2105, 2209
BssNAI GTATAC 3 cut(s) 573, 888, 1536
BssNI GRCGYC 1 cut(s) 712
BssSI CACGAG 1 cut(s) 2687
BssT1I CCWWGG 1 cut(s) 262
Bst1107I GTATAC 3 cut(s) 573, 888, 1536
Bst2BI CACGAG 1 cut(s) 2687
Bst2UI CCWGG 2 cut(s) 2211, 2631
Bst4CI ACNGT 7 cut(s) 146, 385, 1428, 1457, 2323, 2787, 2951
Bst6I CTCTTC 3 cut(s) 807, 953, 1260
BstACI GRCGYC 1 cut(s) 712
BstAFI CTTAAG 1 cut(s) 241
BstAPI GCANNNNNTGC 1 cut(s) 1475
BstBAI YACGTR 1 cut(s) 885
BstC8I GCNNGC 5 cut(s) 2156, 2506, 2709, 2956, 2960
BstDEI CTNAG 9 cut(s) 137, 621, 1068, 1437, 1461, 1563, 1997, 2407, 2775
BstDSI CCRYGG 2 cut(s) 128, 1201
BstEII GGTNACC 1 cut(s) 146
BstF5I GGATG 8 cut(s) 187, 537, 659, 1197, 2007, 2403, 2832, 2895
BstFNI CGCG 2 cut(s) 1203, 2021
BstH2I RGCGCY 1 cut(s) 1148
BstHHI GCGC 1 cut(s) 1147
BstMAI GTCTC 8 cut(s) 172, 499, 510, 620, 804, 1262, 1323, 1853
BstMWI GCNNNNNNNGC 6 cut(s) 941, 1466, 1475, 1831, 2053, 2467
BstNI CCWGG 2 cut(s) 2211, 2631
BstNSI RCATGY 2 cut(s) 365, 1525
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 4 cut(s) 150, 2104, 2209, 2629
BstSFI CTRYAG 2 cut(s) 669, 2468
BstSLI GKGCMC 1 cut(s) 1602
BstUI CGCG 2 cut(s) 1203, 2021
BstV1I GCAGC 3 cut(s) 358, 2096, 2482
BstV2I GAAGAC 3 cut(s) 412, 1385, 1493
BstX2I RGATCY 3 cut(s) 81, 1064, 3079
BstYI RGATCY 3 cut(s) 81, 1064, 3079
BstZ17I GTATAC 3 cut(s) 573, 888, 1536
Bsu36I CCTNAGG 1 cut(s) 2407
BsuI GTATCC 1 cut(s) 753
BtgI CCRYGG 2 cut(s) 128, 1201
BtsCI GGATG 8 cut(s) 187, 537, 659, 1197, 2007, 2403, 2832, 2895
BtsI GCAGTG 1 cut(s) 3073
BtsIMutI CAGTG 4 cut(s) 1433, 2455, 2956, 3073
BtuMI TCGCGA 1 cut(s) 2021
BveI ACCTGC 2 cut(s) 2300, 2351
Cac8I GCNNGC 5 cut(s) 2156, 2506, 2709, 2956, 2960
CaiI CAGNNNCTG 2 cut(s) 668, 2216
CfoI GCGC 1 cut(s) 1147
Cfr10I RCCGGY 1 cut(s) 2933
Cfr13I GGNCC 1 cut(s) 2289
Cfr42I CCGCGG 1 cut(s) 1204
CseI GACGC 2 cut(s) 215, 3005
Csp6I GTAC 3 cut(s) 681, 693, 2733
CviQI GTAC 3 cut(s) 681, 693, 2733
DdeI CTNAG 9 cut(s) 137, 621, 1068, 1437, 1461, 1563, 1997, 2407, 2775
DraI TTTAAA 1 cut(s) 1785
DriI GACNNNNNGTC 1 cut(s) 130
Eam1104I CTCTTC 3 cut(s) 807, 953, 1260
Eam1105I GACNNNNNGTC 1 cut(s) 130
EarI CTCTTC 3 cut(s) 807, 953, 1260
EciI GGCGGA 1 cut(s) 772
Ecl136II GAGCTC 1 cut(s) 903
Eco130I CCWWGG 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 905
Eco31I GGTCTC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 2289
Eco47III AGCGCT 1 cut(s) 1146
Eco53kI GAGCTC 1 cut(s) 903
Eco57I CTGAAG 2 cut(s) 788, 2949
Eco81I CCTNAGG 1 cut(s) 2407
Eco88I CYCGRG 1 cut(s) 977
Eco91I GGTNACC 1 cut(s) 146
EcoICRI GAGCTC 1 cut(s) 903
EcoO65I GGTNACC 1 cut(s) 146
EcoRI GAATTC 2 cut(s) 878, 1333
EcoRII CCWGG 2 cut(s) 2209, 2629
EcoT14I CCWWGG 1 cut(s) 262
EcoT22I ATGCAT 4 cut(s) 1749, 2170, 2403, 2559
EcoT38I GRGCYC 1 cut(s) 905
ErhI CCWWGG 1 cut(s) 262
Esp3I CGTCTC 1 cut(s) 804
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 2 cut(s) 723, 959
FauI CCCGC 2 cut(s) 1196, 2844
FauNDI CATATG 1 cut(s) 2170
FbaI TGATCA 3 cut(s) 1209, 1591, 2445
FblI GTMKAC 5 cut(s) 572, 770, 887, 1535, 2677
Fnu4HI GCNGC 3 cut(s) 372, 2085, 2471
FokI GGATG 8 cut(s) 174, 544, 666, 1184, 2014, 2410, 2819, 2902
FriOI GRGCYC 1 cut(s) 905
Fsp4HI GCNGC 3 cut(s) 372, 2085, 2471
FspBI CTAG 5 cut(s) 78, 702, 1991, 2354, 2640
GlaI GCGC 1 cut(s) 1146
GluI GCNGC 3 cut(s) 372, 2085, 2471
GsaI CCCAGC 1 cut(s) 2889
GsuI CTGGAG 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 1148
HapII CCGG 3 cut(s) 152, 2105, 2934
HgaI GACGC 2 cut(s) 215, 3005
HhaI GCGC 1 cut(s) 1147
Hin1I GRCGYC 1 cut(s) 712
Hin6I GCGC 1 cut(s) 1145
HinP1I GCGC 1 cut(s) 1145
HincII GTYRAC 5 cut(s) 729, 771, 1453, 1756, 1796
HindII GTYRAC 5 cut(s) 729, 771, 1453, 1756, 1796
HindIII AAGCTT 1 cut(s) 2054
HpaI GTTAAC 1 cut(s) 1756
HpaII CCGG 3 cut(s) 152, 2105, 2934
HphI GGTGA 6 cut(s) 397, 962, 1037, 1958, 2746, 3005
Hpy99I CGWCG 4 cut(s) 146, 206, 209, 216
HpyAV CCTTC 9 cut(s) 51, 62, 249, 854, 934, 1012, 1030, 1643, 1937
HpyCH4III ACNGT 7 cut(s) 146, 385, 1428, 1457, 2323, 2787, 2951
HpyCH4IV ACGT 6 cut(s) 248, 712, 731, 884, 890, 1788
HpyF10VI GCNNNNNNNGC 6 cut(s) 941, 1466, 1475, 1831, 2053, 2467
HpyF3I CTNAG 9 cut(s) 137, 621, 1068, 1437, 1461, 1563, 1997, 2407, 2775
HpySE526I ACGT 6 cut(s) 248, 712, 731, 884, 890, 1788
Hsp92I GRCGYC 1 cut(s) 712
HspAI GCGC 1 cut(s) 1145
Ksp22I TGATCA 3 cut(s) 1209, 1591, 2445
KspAI GTTAAC 1 cut(s) 1756
KspI CCGCGG 1 cut(s) 1204
LmnI GCTCC 1 cut(s) 2213
Lsp1109I GCAGC 3 cut(s) 358, 2096, 2482
MaeI CTAG 5 cut(s) 78, 702, 1991, 2354, 2640
MaeII ACGT 6 cut(s) 248, 712, 731, 884, 890, 1788
MbiI CCGCTC 2 cut(s) 2124, 2395
MfeI CAATTG 1 cut(s) 2484
MflI RGATCY 3 cut(s) 81, 1064, 3079
MhlI GDGCHC 2 cut(s) 905, 1602
MlyI GAGTC 8 cut(s) 274, 1536, 1748, 1801, 1929, 1989, 2169, 2673
MmeI TCCRAC 3 cut(s) 142, 482, 631
Mph1103I ATGCAT 4 cut(s) 1749, 2170, 2403, 2559
MroXI GAANNNNTTC 2 cut(s) 878, 1470
MslI CAYNNNNRTG 3 cut(s) 2234, 2546, 2552
MspA1I CMGCKG 3 cut(s) 1187, 1203, 2473
MspCI CTTAAG 1 cut(s) 241
MspI CCGG 3 cut(s) 152, 2105, 2934
MspR9I CCNGG 4 cut(s) 152, 2106, 2211, 2631
MunI CAATTG 1 cut(s) 2484
Mva1269I GAATGC 1 cut(s) 2245
MvaI CCWGG 2 cut(s) 2211, 2631
MvnI CGCG 2 cut(s) 1203, 2021
MwoI GCNNNNNNNGC 6 cut(s) 941, 1466, 1475, 1831, 2053, 2467
NciI CCSGG 2 cut(s) 152, 2106
NdeI CATATG 1 cut(s) 2170
NlaIV GGNNCC 1 cut(s) 260
NmuCI GTSAC 6 cut(s) 8, 132, 396, 985, 2204, 2993
NruI TCGCGA 1 cut(s) 2021
NsiI ATGCAT 4 cut(s) 1749, 2170, 2403, 2559
NspI RCATGY 2 cut(s) 365, 1525
PciI ACATGT 1 cut(s) 361
PctI GAATGC 1 cut(s) 2245
PdmI GAANNNNTTC 2 cut(s) 878, 1470
PfeI GAWTC 8 cut(s) 216, 1074, 1180, 2093, 2186, 2425, 2761, 3009
PkrI GCNGC 3 cut(s) 373, 2086, 2472
PleI GAGTC 8 cut(s) 274, 1536, 1747, 1800, 1928, 1989, 2169, 2673
PpsI GAGTC 8 cut(s) 274, 1536, 1747, 1800, 1928, 1989, 2169, 2673
Ppu21I YACGTR 1 cut(s) 885
PscI ACATGT 1 cut(s) 361
Psp124BI GAGCTC 1 cut(s) 905
Psp6I CCWGG 2 cut(s) 2209, 2629
PspEI GGTNACC 1 cut(s) 146
PspFI CCCAGC 1 cut(s) 2885
PspGI CCWGG 2 cut(s) 2209, 2629
PspN4I GGNNCC 1 cut(s) 260
PspPI GGNCC 1 cut(s) 2289
PstI CTGCAG 1 cut(s) 2472
PstNI CAGNNNCTG 2 cut(s) 668, 2216
PsuI RGATCY 3 cut(s) 81, 1064, 3079
PvuII CAGCTG 2 cut(s) 1187, 2473
RruI TCGCGA 1 cut(s) 2021
RsaI GTAC 3 cut(s) 682, 694, 2734
RsaNI GTAC 3 cut(s) 681, 693, 2733
RseI CAYNNNNRTG 3 cut(s) 2234, 2546, 2552
SacI GAGCTC 1 cut(s) 905
SacII CCGCGG 1 cut(s) 1204
SalI GTCGAC 1 cut(s) 769
SatI GCNGC 3 cut(s) 372, 2085, 2471
Sau96I GGNCC 1 cut(s) 2289
SchI GAGTC 8 cut(s) 274, 1536, 1748, 1801, 1929, 1989, 2169, 2673
ScrFI CCNGG 4 cut(s) 152, 2106, 2211, 2631
SduI GDGCHC 2 cut(s) 905, 1602
SfcI CTRYAG 2 cut(s) 669, 2468
Sfr303I CCGCGG 1 cut(s) 1204
SgrBI CCGCGG 1 cut(s) 1204
SinI GGWCC 1 cut(s) 2289
SmiMI CAYNNNNRTG 3 cut(s) 2234, 2546, 2552
SmlI CTYRAG 4 cut(s) 67, 241, 1320, 1668
SmoI CTYRAG 4 cut(s) 67, 241, 1320, 1668
SsiI CCGC 7 cut(s) 757, 1201, 1203, 2122, 2395, 2837, 2962
SspI AATATT 1 cut(s) 48
SspMI CTAG 5 cut(s) 78, 702, 1991, 2354, 2640
SstI GAGCTC 1 cut(s) 905
StyD4I CCNGG 4 cut(s) 150, 2104, 2209, 2629
StyI CCWWGG 1 cut(s) 262
TaaI ACNGT 7 cut(s) 146, 385, 1428, 1457, 2323, 2787, 2951
TaiI ACGT 6 cut(s) 251, 715, 734, 887, 893, 1791
TaqI TCGA 6 cut(s) 211, 770, 1484, 1918, 2254, 2423
TaqII GACCGA 1 cut(s) 196
TatI WGTACW 2 cut(s) 692, 2732
TfiI GAWTC 8 cut(s) 216, 1074, 1180, 2093, 2186, 2425, 2761, 3009
TscAI CASTG 4 cut(s) 1433, 2455, 2956, 3073
TseFI GTSAC 6 cut(s) 8, 132, 396, 985, 2204, 2993
TseI GCWGC 3 cut(s) 371, 2084, 2470
Tsp45I GTSAC 6 cut(s) 8, 132, 396, 985, 2204, 2993
TspGWI ACGGA 2 cut(s) 1142, 2302
TspRI CASTG 4 cut(s) 1433, 2455, 2956, 3073
Vha464I CTTAAG 1 cut(s) 241
VneI GTGCAC 1 cut(s) 1598
VpaK11BI GGWCC 1 cut(s) 2289
XapI RAATTY 6 cut(s) 878, 1282, 1333, 1849, 2385, 2535
XceI RCATGY 2 cut(s) 365, 1525
XmiI GTMKAC 5 cut(s) 572, 770, 887, 1535, 2677
XmnI GAANNNNTTC 2 cut(s) 878, 1470
XspI CTAG 5 cut(s) 78, 702, 1991, 2354, 2640
ZraI GACGTC 1 cut(s) 713
Zsp2I ATGCAT 4 cut(s) 1749, 2170, 2403, 2559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.