Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0009191

Physical Location & Seq

Genomic Coordinates Forward (+)

32306960 .. 32312327

Gene Structure 5368 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1235800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3228 bp

ATGGAGTCGCTGACAACGTACTCCTCTTCCTTGTTGTGCACCTGTCCACTGCTGTCCTCCCATTGTTCCCAATCCAACACCACCGCCTTCAAAATTCCCTCGACCTCCCCCAAACCTAAAACCACCTTCACCAACATTGTAGTACAGTCCTCGGTGAGCCCGGACCCCTGGTCTCTCAGCGACGGCAACAACCCCGACCGCCCCAAACCCAGGTCCAAGAACCCCAAAAAGCCCCTCTCCGACGACAACGCCCGCCGCATCATCAAGGCCAAGGCCAACTACCTCAGCGCCCTCCGCCGGAACCAGGGCCCACAGGCCCAAACCCCCAAGTGGATTAAGCGGACCCCCGAGCAGATGGTCAGCTACTTGCACGACGACAGGAACGGCCACCTCTACGGCCGCCATGTCGTCGCCGCCATTAAGCACGTTAGAGCGCTGTCCGAGAAGTCCGAGGGGGCGTACGACATGCGCGCCGTCATGGCCTCCTTCGTCGGGAAGCTCAGCTTCAGAGAAATGTGTGTGGTGCTCAAGGAGCAGAAGAGCTGGAGGCAAGTCAGGGACTTGTTCTCTTGGATGAAATTGCAGTTAAGCTACCGCCCAAGTGTTATTGTCTACACGTTAGTATTGCGGGCATATGGCCAGGTCGGGAAGATCAAGCTGGCTGAGCAAACCTTCTTGGAGATGCTCGAATCTGGGTGTGAACCGGATGAAGTTGCTTGCGGTACCATGCTCTGTACATACGCCAGATGGGGGCGTCACAAGGCTATGCTGGCGTTTTATTCAGCTGTGCAAGAAAGGGGGATTCTACTTTCTGTTGCTGTTTATAATTTTATGTTGTCCTCCTTGCAGAAGAAATCACTCCACGGAATGGTCATAGAAATGTGGAGGCAGATGGTGGACATTGAAGTGGTACCTAATAAATTTACTTATACGGTAGTAATTGGCTCCCTCGTCAAAGAAGGCCTCCACGAGGAGGCTCTTAAGAATTTCATTGAGTTGAAGAATGCTGGAATCGTTCCTGAGGAGGCAACTTATAGTTTGCTCATCAGTTTAAGCACCAAAAATGGCAAATTTGATGAAGCTCTTAGATTATATGAAGACATGAGGTCTCTAGGAATAGTTCCGAGCAACTACACTTGTGCTTCACTTCTGACGTTGTATTACAAGAAGGAGGATTACTCCAAAGCCCTTTCTCTCTTTTCAGAAATGGAAAGGAAGAAAATTGCAGCGGATGAAGTGATCTACGGTTTGCTCATTAGAATATATGGAAAACTTGGTCTTTACGAGGATGCTCAGACAGCTTTTGAAGAGATGGAGCAGCTGGGTTTACTTAGTGACCAGAAAACATATTTGGCAATGACGCAAGTCCATCTCAATTCAGGGAATTGCGACAAAGCTTTAGAAGTTATGGAACTAATGAAGTCTAGAAAAAACATTTGGCTATCAAGATTTGCTTATATTGTCTTACTGCAGTGTTATGTTATGAAGGAGGATCTGAGTTCTGCTGAAGTAACATTTCAGGCTCTATCCAAGACTGGACTCCCTGATGCAGGCTCTTGCAATGATATGCTCAATTTGTATATAAAACTGAACTTATTGGAAAAGGCTAAGGATTTTATTACCCAGATAAGAAGAGACCGAGTAGATTTTGACGAGGAGCTTTGCAGGACAGTTATGAGAGTATATTGCAAGGAGGGAATGCTTAGAGATGCTGAACAGTTTGTAGAAGAACTGGGTACGAGCGGAGTATGCCAGGATAGTAGATTCGTCCAAACAATTTCTAGTGTTATGTGTGAACACAAGGAAGGAAAATTTGTGACTTTTGACCAGCCTGACACCGTGGCCCTGGGGCTGGTGCTCAGTCTCTATCTGACAGATGGCGATATCAGTAAGACAAAAAAAGTTCTAGCATCATTGCCGGTGACTTCTGTTGGCTTATCAATTGCTAGTCAGCTCATTAAGAACATTATTAGAGAAGGTGATGCATTTAAAGCAGAAACTCATATCAATCAATTGGCCAAGCTTGGCTGCAGGGTGGATGATGCCACGGTTGCTTCTGTGATTAGTTTATATGGAAAGAAACGCAAGCTGATGAAGGCCCTGGAAATATTTACAGCATTTGCAGATTCTCCTTTAGCCAAAAAATTACTATGTAACTCAATGCTTGATGCATATGCCAAATGTGGTAAACCACAGGAGGCATACGCACTTTACAAACAGCTTACAGAGGAAGGACACGATCTGGATGCTGTTGCCATCAGCATAGTTGTCAATGCTTTGACTAACAGTGGGAAACATAGAGAAGCAGAGAATGTCATCCGGGAAAGCCTTGAGCACCACTTGGAGCTAGATACTGTGGCGTACAATACTTTTATTAAGGCTATGCTGGAAGCAGGTAGGTTGCATTTTGCATCCAGTATCTATGAGCGCATGCTTTCTGAGGGGGTTGCTCCATCAATTCGGACATATAGCACCATGATCAGTGTTTACGGTCGAGGTCGAAAGCTGGAAAAGGCTGTAGAGATGTTCACTACGGCTCGCAACTTGGGTTTGTCTTTGGATGAGAAGGCATATATGAATCTGGTTAGCTACTATGGGAAAGCTGGAAAAAGGCATGAAGCATCCATGCTATTCAGCAAAATGCGAGAAGAAGGGATAAAACCTGGGATGGTCAGTTATAATATAATGATCAATGTATATGCTGCTGGGGGCCTTTATCAAGAAGCAGAGGAATTATTCAAAGCCATGCAGCGAGATGGTTGCTTACCCGACTCCTTTACCTACCTGTCCCTAATTCGAGCCTACACAGAGAGTCTGAAATACTCAGAAGCAGAGGAAACCATTAACTCTATGCATGAAAACGGTGTCCATCCCTCCTGTGCACATTTTAACCTTTTACTCTCCGCTTTTGCCAAAATGGGTTTGATAGGTGAAGCAGAAAGAATTTATAAAGAACTACATGGAGCTGGTTTGAACCCTGACGTGGCATGTTATCAGACTATGCTGAGAGGTTACATGGACTATGGACATTTAGAAGAAGGGATCAAGCTTTTTGAACAGATCAGTAAGTCTGGAGAGGCAGACAGGTTTATTTTGAGCGCAGCAGTGCATTGTTATAAATCTGTGGGAAAGGAACTTGAAGCTGAAAATGTTTTGCATTCCATGAGTAATTTGGGAATTTCATTTCTGGAGAACCTAGAAATTGGATCCAAGCTAAAGACCTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

1076

Amino Acids

120.74

Weight (kDa)

7.87

Isoelectric Point (pI)

32.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	200 - 248	2.4e-08	PPR repeat family
PPR_long	PF17177	255 - 386	6.2e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	271 - 319	2.3e-06	PPR repeat family
PPR_long	PF17177	290 - 426	3.6e-10	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	305 - 350	1.3e-08	PPR repeat family
PPR	PF01535	344 - 373	6.7e-06	PPR repeat
PPR_2	PF13041	348 - 384	2.5e-06	PPR repeat family
PPR_3	PF13812	363 - 424	7.9e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	718 - 761	1.2e-06	PPR repeat family
PPR_long	PF17177	725 - 883	2.9e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	773 - 831	3.8e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	784 - 830	1.6e-10	PPR repeat family
PPR_3	PF13812	846 - 900	5.3e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	855 - 901	6.7e-10	PPR repeat family
PPR_long	PF17177	876 - 1010	1.5e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	888 - 935	7.2e-13	PPR repeat family
PPR	PF01535	891 - 921	1.7e-08	PPR repeat
PPR_3	PF13812	912 - 972	1.2e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	963 - 1005	2.9e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27270 AT5G27270 AT5G27270 AT5G27270 AT5G27270
fragaria_vesca	FvH4_7g33960 FvH4_7g33960
malus_domestica	MD01G1235800.v1.1
prunus_persica	Prupe.2G325900_v2.0.a1 Prupe.2G325900_v2.0.a1
pyrus_communis	pycom01g24390
rosa_chinensis	RchiOBHm_Chr1g0384191
rosa_laevigata	RLG00000026061
rosa_multiflora	Rmu_sc0005139.1_g000006 Rmu_sc0014578.1_g000006
rosa_roxburghii	Rroxscaffold_4G00276870
rosa_rugosa	Rorug01G0445600
rosa_samantha	Rh1AG471100 Rh1BG428800 Rh1CG441500 Rh1DG461600
rosa_wichuraiana	Rw1G040140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	825, 2679, 2949, 3117
Acc36I	ACCTGC	1 cut(s)	2384
Acc65I	GGTACC	2 cut(s)	722, 910
AccB1I	GGYRCC	2 cut(s)	722, 910
AccB7I	CCANNNNNTGG	1 cut(s)	868
AccBSI	CCGCTC	1 cut(s)	1743
AccI	GTMKAC	1 cut(s)	612
AccII	CGCG	1 cut(s)	471
AclWI	GGATC	4 cut(s)	1500, 3050, 3201, 3214
AcoI	YGGCCR	4 cut(s)	385, 397, 637, 2016
AcsI	RAATTY	7 cut(s)	93, 920, 985, 1070, 1811, 2943, 3177
AcuI	CTGAAG	2 cut(s)	490, 1527
AcyI	GRCGYC	1 cut(s)	754
AfaI	GTAC	8 cut(s)	20, 144, 461, 724, 736, 912, 1738, 2363
AfeI	AGCGCT	1 cut(s)	435
AflII	CTTAAG	1 cut(s)	980
AflIII	ACRYGT	1 cut(s)	615
AgsI	TTSAA	8 cut(s)	91, 905, 1000, 1307, 2740, 2974, 3056, 3140
AhdI	GACNNNNNGTC	2 cut(s)	169, 1105
AjiI	CACGTC	1 cut(s)	2983
AjnI	CCWGG	8 cut(s)	167, 209, 303, 639, 1752, 1845, 2100, 2662
AjuI	GAANNNNNNNTTGG	4 cut(s)	1334, 1366, 1420, 1452
Alw21I	GWGCWC	5 cut(s)	41, 528, 1860, 2337, 2884
Alw26I	GTCTC	4 cut(s)	177, 1113, 1629, 1868
Alw44I	GTGCAC	2 cut(s)	37, 2880
AlwI	GGATC	4 cut(s)	1500, 3050, 3201, 3214
Ama87I	CYCGRG	1 cut(s)	347
Aor51HI	AGCGCT	1 cut(s)	435
ApaI	GGGCCC	1 cut(s)	311
ApaLI	GTGCAC	2 cut(s)	37, 2880
ApeKI	GCWGC	6 cut(s)	1226, 1318, 2028, 2702, 2749, 3101
ApoI	RAATTY	7 cut(s)	93, 920, 985, 1070, 1811, 2943, 3177
Asp718I	GGTACC	2 cut(s)	722, 910
AspLEI	GCGC	6 cut(s)	290, 436, 471, 473, 2430, 3101
AspS9I	GGNCC	9 cut(s)	163, 213, 307, 308, 316, 342, 1843, 2098, 2710
AsuC2I	CCSGG	2 cut(s)	161, 2321
AsuHPI	GGTGA	5 cut(s)	121, 166, 1933, 1991, 2942
AvaI	CYCGRG	1 cut(s)	347
AvaII	GGWCC	3 cut(s)	163, 213, 342
AxyI	CCTNAGG	1 cut(s)	1020
BaeGI	GKGCMC	3 cut(s)	41, 311, 2884
BalI	TGGCCA	2 cut(s)	639, 2018
BamHI	GGATCC	1 cut(s)	3206
BanI	GGYRCC	2 cut(s)	722, 910
BanII	GRGCYC	2 cut(s)	161, 311
BarI	GAAGNNNNNNTAC	2 cut(s)	1160, 1192
BauI	CACGAG	1 cut(s)	968
BbsI	GAAGAC	1 cut(s)	1104
Bbv12I	GWGCWC	5 cut(s)	41, 528, 1860, 2337, 2884
BbvCI	CCTCAGC	1 cut(s)	284
BbvI	GCAGC	6 cut(s)	1238, 1330, 2015, 2689, 2761, 3113
BceAI	ACGGC	5 cut(s)	199, 400, 412, 458, 2550
BcgI	CGANNNNNNTGC	4 cut(s)	2228, 2262, 2742, 2776
BciT130I	CCWGG	8 cut(s)	169, 211, 305, 641, 1754, 1847, 2102, 2664
BclI	TGATCA	2 cut(s)	2478, 2688
BcnI	CCSGG	2 cut(s)	161, 2321
BcoDI	GTCTC	4 cut(s)	177, 1113, 1629, 1868
BfaI	CTAG	7 cut(s)	1112, 1425, 1782, 1907, 1947, 2348, 3197
BfmI	CTRYAG	3 cut(s)	1469, 2029, 2517
BfoI	RGCGCY	2 cut(s)	291, 437
BfrI	CTTAAG	1 cut(s)	980
BfuAI	ACCTGC	1 cut(s)	2384
BglI	GCCNNNNNGGC	1 cut(s)	479
BisI	GCNGC	9 cut(s)	256, 400, 414, 1227, 1319, 2029, 2703, 2750, 3102
BlpI	GCTNAGC	2 cut(s)	500, 663
BlsI	GCNGC	9 cut(s)	257, 401, 415, 1228, 1320, 2030, 2704, 2751, 3103
Bme18I	GGWCC	3 cut(s)	163, 213, 342
BmeRI	GACNNNNNGTC	2 cut(s)	169, 1105
BmeT110I	CYCGRG	1 cut(s)	347
BmgBI	CACGTC	1 cut(s)	2983
BmgT120I	GGNCC	9 cut(s)	163, 213, 307, 308, 316, 342, 1843, 2098, 2710
BmiI	GGNNCC	9 cut(s)	165, 302, 309, 344, 724, 912, 946, 2711, 3208
BmrI	ACTGGG	1 cut(s)	1742
BmuI	ACTGGG	1 cut(s)	1742
BoxI	GACNNNNGTC	1 cut(s)	1364
BpiI	GAAGAC	1 cut(s)	1104
BplI	GAGNNNNNCTC	2 cut(s)	1163, 1195
BpmI	CTGGAG	3 cut(s)	565, 3093, 3209
Bpu10I	CCTNAGC	2 cut(s)	284, 1608
Bpu1102I	GCTNAGC	2 cut(s)	500, 663
BpuEI	CTTGAG	2 cut(s)	512, 2351
BpuMI	CCSGG	2 cut(s)	161, 2321
BsaHI	GRCGYC	1 cut(s)	754
BsaI	GGTCTC	3 cut(s)	177, 1113, 1629
BsaWI	WCCGGW	1 cut(s)	703
Bse118I	RCCGGY	1 cut(s)	1918
Bse1I	ACTGG	3 cut(s)	1540, 1737, 2415
Bse21I	CCTNAGG	1 cut(s)	1020
Bse3DI	GCAATG	3 cut(s)	1362, 1567, 1913
BseBI	CCWGG	8 cut(s)	169, 211, 305, 641, 1754, 1847, 2102, 2664
BseMI	GCAATG	3 cut(s)	1362, 1567, 1913
BseNI	ACTGG	3 cut(s)	1540, 1737, 2415
BsePI	GCGCGC	1 cut(s)	469
BseRI	GAGGAG	4 cut(s)	13, 986, 1037, 1670
BseSI	GKGCMC	3 cut(s)	41, 311, 2884
BseX3I	CGGCCG	1 cut(s)	397
BseXI	GCAGC	6 cut(s)	1238, 1330, 2015, 2689, 2761, 3113
BseYI	CCCAGC	2 cut(s)	1321, 2705
Bsh1236I	CGCG	1 cut(s)	471
Bsh1285I	CGRYCG	3 cut(s)	199, 400, 2494
BshNI	GGYRCC	2 cut(s)	722, 910
BsiEI	CGRYCG	3 cut(s)	199, 400, 2494
BsiHKAI	GWGCWC	5 cut(s)	41, 528, 1860, 2337, 2884
BsiHKCI	CYCGRG	1 cut(s)	347
BsiSI	CCGG	5 cut(s)	161, 298, 704, 1919, 2320
BsiWI	CGTACG	1 cut(s)	459
BslFI	GGGAC	2 cut(s)	572, 2773
BsmAI	GTCTC	4 cut(s)	177, 1113, 1629, 1868
BsmFI	GGGAC	2 cut(s)	572, 2773
BsmI	GAATGC	3 cut(s)	1009, 1704, 3157
Bso31I	GGTCTC	3 cut(s)	177, 1113, 1629
BsoBI	CYCGRG	1 cut(s)	347
Bsp120I	GGGCCC	1 cut(s)	307
Bsp1286I	GDGCHC	7 cut(s)	41, 161, 311, 528, 1860, 2337, 2884
Bsp1407I	TGTACA	1 cut(s)	734
Bsp143I	GATC	9 cut(s)	651, 1239, 1492, 2239, 2478, 2688, 3042, 3060, 3206
Bsp1720I	GCTNAGC	2 cut(s)	500, 663
BspFNI	CGCG	1 cut(s)	471
BspLI	GGNNCC	9 cut(s)	165, 302, 309, 344, 724, 912, 946, 2711, 3208
BspMAI	CTGCAG	2 cut(s)	1473, 2033
BspMI	ACCTGC	1 cut(s)	2384
BspPI	GGATC	4 cut(s)	1500, 3050, 3201, 3214
BspQI	GCTCTTC	1 cut(s)	533
BspT107I	GGYRCC	2 cut(s)	722, 910
BspTI	CTTAAG	1 cut(s)	980
BspTNI	GGTCTC	3 cut(s)	177, 1113, 1629
BsrBI	CCGCTC	1 cut(s)	1743
BsrDI	GCAATG	3 cut(s)	1362, 1567, 1913
BsrFI	RCCGGY	1 cut(s)	1918
BsrGI	TGTACA	1 cut(s)	734
BsrI	ACTGG	3 cut(s)	1540, 1737, 2415
BssAI	RCCGGY	1 cut(s)	1918
BssHII	GCGCGC	1 cut(s)	469
BssMI	GATC	9 cut(s)	651, 1239, 1492, 2239, 2478, 2688, 3042, 3060, 3206
BssNI	GRCGYC	1 cut(s)	754
BssSI	CACGAG	1 cut(s)	968
BssT1I	CCWWGG	1 cut(s)	270
Bst2BI	CACGAG	1 cut(s)	968
Bst2UI	CCWGG	8 cut(s)	169, 211, 305, 641, 1754, 1847, 2102, 2664
Bst6I	CTCTTC	4 cut(s)	31, 533, 1302, 1627
BstACI	GRCGYC	1 cut(s)	754
BstAFI	CTTAAG	1 cut(s)	980
BstAUI	TGTACA	1 cut(s)	734
BstDSI	CCRYGG	3 cut(s)	862, 1839, 2046
BstENI	CCTNNNNNAGG	2 cut(s)	968, 1548
BstFNI	CGCG	1 cut(s)	471
BstH2I	RGCGCY	2 cut(s)	291, 437
BstHHI	GCGC	6 cut(s)	290, 436, 471, 473, 2430, 3101
BstKTI	GATC	9 cut(s)	654, 1242, 1495, 2242, 2481, 2691, 3045, 3063, 3209
BstMAI	GTCTC	4 cut(s)	177, 1113, 1629, 1868
BstMBI	GATC	9 cut(s)	651, 1239, 1492, 2239, 2478, 2688, 3042, 3060, 3206
BstMCI	CGRYCG	3 cut(s)	199, 400, 2494
BstMWI	GCNNNNNNNGC	9 cut(s)	294, 479, 532, 664, 770, 1298, 1749, 1991, 2051
BstNI	CCWGG	8 cut(s)	169, 211, 305, 641, 1754, 1847, 2102, 2664
BstNSI	RCATGY	3 cut(s)	469, 2434, 2991
BstPAI	GACNNNNGTC	1 cut(s)	1364
BstSFI	CTRYAG	3 cut(s)	1469, 2029, 2517
BstSLI	GKGCMC	3 cut(s)	41, 311, 2884
BstUI	CGCG	1 cut(s)	471
BstV1I	GCAGC	6 cut(s)	1238, 1330, 2015, 2689, 2761, 3113
BstV2I	GAAGAC	1 cut(s)	1104
BstX2I	RGATCY	2 cut(s)	1492, 3206
BstYI	RGATCY	2 cut(s)	1492, 3206
BstZI	CGGCCG	1 cut(s)	397
Bsu36I	CCTNAGG	1 cut(s)	1020
BtgI	CCRYGG	3 cut(s)	862, 1839, 2046
BtrI	CACGTC	1 cut(s)	2983
BtsI	GCAGTG	3 cut(s)	47, 1478, 3111
BtsIMutI	CAGTG	5 cut(s)	47, 1478, 2293, 2488, 3111
BveI	ACCTGC	1 cut(s)	2384
CfoI	GCGC	6 cut(s)	290, 436, 471, 473, 2430, 3101
Cfr10I	RCCGGY	1 cut(s)	1918
Cfr13I	GGNCC	9 cut(s)	163, 213, 307, 308, 316, 342, 1843, 2098, 2710
CseI	GACGC	2 cut(s)	743, 1369
Csp6I	GTAC	8 cut(s)	19, 143, 460, 723, 735, 911, 1737, 2362
CviQI	GTAC	8 cut(s)	19, 143, 460, 723, 735, 911, 1737, 2362
DpnI	GATC	9 cut(s)	653, 1241, 1494, 2241, 2480, 2690, 3044, 3062, 3208
DpnII	GATC	9 cut(s)	651, 1239, 1492, 2239, 2478, 2688, 3042, 3060, 3206
DraI	TTTAAA	1 cut(s)	1990
DriI	GACNNNNNGTC	2 cut(s)	169, 1105
EaeI	YGGCCR	4 cut(s)	385, 397, 637, 2016
EagI	CGGCCG	1 cut(s)	397
Eam1104I	CTCTTC	4 cut(s)	31, 533, 1302, 1627
Eam1105I	GACNNNNNGTC	2 cut(s)	169, 1105
EarI	CTCTTC	4 cut(s)	31, 533, 1302, 1627
EciI	GGCGGA	1 cut(s)	284
EclXI	CGGCCG	1 cut(s)	397
Eco130I	CCWWGG	1 cut(s)	270
Eco147I	AGGCCT	1 cut(s)	963
Eco24I	GRGCYC	2 cut(s)	161, 311
Eco31I	GGTCTC	3 cut(s)	177, 1113, 1629
Eco32I	GATATC	1 cut(s)	1885
Eco47I	GGWCC	3 cut(s)	163, 213, 342
Eco47III	AGCGCT	1 cut(s)	435
Eco52I	CGGCCG	1 cut(s)	397
Eco57I	CTGAAG	2 cut(s)	490, 1527
Eco81I	CCTNAGG	1 cut(s)	1020
Eco88I	CYCGRG	1 cut(s)	347
EcoNI	CCTNNNNNAGG	2 cut(s)	968, 1548
EcoO109I	RGGNCCY	3 cut(s)	307, 2098, 2710
EcoRII	CCWGG	8 cut(s)	167, 209, 303, 639, 1752, 1845, 2100, 2662
EcoRV	GATATC	1 cut(s)	1885
EcoT14I	CCWWGG	1 cut(s)	270
EcoT22I	ATGCAT	3 cut(s)	1987, 2173, 2856
EcoT38I	GRGCYC	2 cut(s)	161, 311
ErhI	CCWWGG	1 cut(s)	270
FalI	AAGNNNNNCTT	4 cut(s)	488, 520, 1438, 1470
FaqI	GGGAC	2 cut(s)	572, 2773
FauI	CCCGC	2 cut(s)	260, 621
FauNDI	CATATG	2 cut(s)	634, 2173
FbaI	TGATCA	2 cut(s)	2478, 2688
FblI	GTMKAC	1 cut(s)	612
Fnu4HI	GCNGC	9 cut(s)	256, 400, 414, 1227, 1319, 2029, 2703, 2750, 3102
FriOI	GRGCYC	2 cut(s)	161, 311
Fsp4HI	GCNGC	9 cut(s)	256, 400, 414, 1227, 1319, 2029, 2703, 2750, 3102
FspBI	CTAG	7 cut(s)	1112, 1425, 1782, 1907, 1947, 2348, 3197
GlaI	GCGC	6 cut(s)	289, 435, 470, 472, 2429, 3100
GluI	GCNGC	9 cut(s)	256, 400, 414, 1227, 1319, 2029, 2703, 2750, 3102
GsaI	CCCAGC	2 cut(s)	1325, 2709
GsuI	CTGGAG	3 cut(s)	565, 3093, 3209
HaeII	RGCGCY	2 cut(s)	291, 437
HapII	CCGG	5 cut(s)	161, 298, 704, 1919, 2320
HgaI	GACGC	2 cut(s)	743, 1369
HhaI	GCGC	6 cut(s)	290, 436, 471, 473, 2430, 3101
Hin1I	GRCGYC	1 cut(s)	754
Hin6I	GCGC	6 cut(s)	288, 434, 469, 471, 2428, 3099
HinP1I	GCGC	6 cut(s)	288, 434, 469, 471, 2428, 3099
HindIII	AAGCTT	3 cut(s)	1395, 2021, 3047
HpaII	CCGG	5 cut(s)	161, 298, 704, 1919, 2320
HphI	GGTGA	5 cut(s)	121, 166, 1933, 1991, 2942
Hpy188III	TCNNGA	9 cut(s)	493, 646, 1019, 1425, 1446, 2243, 2720, 3072, 3188
Hpy99I	CGWCG	5 cut(s)	185, 245, 377, 413, 494
HpyCH4IV	ACGT	5 cut(s)	17, 426, 617, 1154, 2982
HpyF10VI	GCNNNNNNNGC	9 cut(s)	294, 479, 532, 664, 770, 1298, 1749, 1991, 2051
HpySE526I	ACGT	5 cut(s)	17, 426, 617, 1154, 2982
Hsp92I	GRCGYC	1 cut(s)	754
HspAI	GCGC	6 cut(s)	288, 434, 469, 471, 2428, 3099
KpnI	GGTACC	2 cut(s)	726, 914
Ksp22I	TGATCA	2 cut(s)	2478, 2688
Kzo9I	GATC	9 cut(s)	651, 1239, 1492, 2239, 2478, 2688, 3042, 3060, 3206
LguI	GCTCTTC	1 cut(s)	533
LmnI	GCTCC	7 cut(s)	532, 950, 1315, 1657, 2344, 2455, 2963
Lsp1109I	GCAGC	6 cut(s)	1238, 1330, 2015, 2689, 2761, 3113
MaeI	CTAG	7 cut(s)	1112, 1425, 1782, 1907, 1947, 2348, 3197
MaeII	ACGT	5 cut(s)	17, 426, 617, 1154, 2982
MaeIII	GTNAC	7 cut(s)	755, 1334, 1510, 1816, 1921, 2153, 3011
MalI	GATC	9 cut(s)	653, 1241, 1494, 2241, 2480, 2690, 3044, 3062, 3208
MbiI	CCGCTC	1 cut(s)	1743
MboI	GATC	9 cut(s)	651, 1239, 1492, 2239, 2478, 2688, 3042, 3060, 3206
MfeI	CAATTG	2 cut(s)	1941, 2012
MflI	RGATCY	2 cut(s)	1492, 3206
MhlI	GDGCHC	7 cut(s)	41, 161, 311, 528, 1860, 2337, 2884
MlsI	TGGCCA	2 cut(s)	639, 2018
MluNI	TGGCCA	2 cut(s)	639, 2018
MlyI	GAGTC	4 cut(s)	14, 1533, 2765, 2821
MmeI	TCCRAC	2 cut(s)	99, 264
Mox20I	TGGCCA	2 cut(s)	639, 2018
Mph1103I	ATGCAT	3 cut(s)	1987, 2173, 2856
MscI	TGGCCA	2 cut(s)	639, 2018
MslI	CAYNNNNRTG	2 cut(s)	878, 905
Msp20I	TGGCCA	2 cut(s)	639, 2018
MspA1I	CMGCKG	3 cut(s)	785, 1229, 1321
MspCI	CTTAAG	1 cut(s)	980
MspI	CCGG	5 cut(s)	161, 298, 704, 1919, 2320
MunI	CAATTG	2 cut(s)	1941, 2012
Mva1269I	GAATGC	3 cut(s)	1009, 1704, 3157
MvaI	CCWGG	8 cut(s)	169, 211, 305, 641, 1754, 1847, 2102, 2664
MvnI	CGCG	1 cut(s)	471
MwoI	GCNNNNNNNGC	9 cut(s)	294, 479, 532, 664, 770, 1298, 1749, 1991, 2051
NciI	CCSGG	2 cut(s)	161, 2321
NdeI	CATATG	2 cut(s)	634, 2173
NdeII	GATC	9 cut(s)	651, 1239, 1492, 2239, 2478, 2688, 3042, 3060, 3206
NlaIV	GGNNCC	9 cut(s)	165, 302, 309, 344, 724, 912, 946, 2711, 3208
NmuCI	GTSAC	4 cut(s)	755, 1334, 1816, 1921
NsiI	ATGCAT	3 cut(s)	1987, 2173, 2856
NspI	RCATGY	3 cut(s)	469, 2434, 2991
PaeI	GCATGC	1 cut(s)	2434
PasI	CCCWGGG	1 cut(s)	1846
PauI	GCGCGC	1 cut(s)	469
PceI	AGGCCT	1 cut(s)	963
PciSI	GCTCTTC	1 cut(s)	533
PcsI	WCGNNNNNNNCGW	1 cut(s)	14
PctI	GAATGC	3 cut(s)	1009, 1704, 3157
PfeI	GAWTC	6 cut(s)	689, 802, 1011, 1764, 2126, 2578
Pfl23II	CGTACG	1 cut(s)	459
PflMI	CCANNNNNTGG	1 cut(s)	868
PfoI	TCCNGGA	1 cut(s)	2319
PkrI	GCNGC	9 cut(s)	257, 401, 415, 1228, 1320, 2030, 2704, 2751, 3103
PleI	GAGTC	4 cut(s)	13, 1533, 2765, 2820
PpsI	GAGTC	4 cut(s)	13, 1533, 2765, 2820
PshAI	GACNNNNGTC	1 cut(s)	1364
PsiI	TTATAA	4 cut(s)	825, 2679, 2949, 3117
Psp6I	CCWGG	8 cut(s)	167, 209, 303, 639, 1752, 1845, 2100, 2662
PspFI	CCCAGC	2 cut(s)	1321, 2705
PspGI	CCWGG	8 cut(s)	167, 209, 303, 639, 1752, 1845, 2100, 2662
PspLI	CGTACG	1 cut(s)	459
PspN4I	GGNNCC	9 cut(s)	165, 302, 309, 344, 724, 912, 946, 2711, 3208
PspOMI	GGGCCC	1 cut(s)	307
PspPI	GGNCC	9 cut(s)	163, 213, 307, 308, 316, 342, 1843, 2098, 2710
PstI	CTGCAG	2 cut(s)	1473, 2033
PsuI	RGATCY	2 cut(s)	1492, 3206
PteI	GCGCGC	1 cut(s)	469
PvuII	CAGCTG	2 cut(s)	785, 1321
RsaI	GTAC	8 cut(s)	20, 144, 461, 724, 736, 912, 1738, 2363
RsaNI	GTAC	8 cut(s)	19, 143, 460, 723, 735, 911, 1737, 2362
RseI	CAYNNNNRTG	2 cut(s)	878, 905
SapI	GCTCTTC	1 cut(s)	533
SatI	GCNGC	9 cut(s)	256, 400, 414, 1227, 1319, 2029, 2703, 2750, 3102
Sau3AI	GATC	9 cut(s)	651, 1239, 1492, 2239, 2478, 2688, 3042, 3060, 3206
Sau96I	GGNCC	9 cut(s)	163, 213, 307, 308, 316, 342, 1843, 2098, 2710
SchI	GAGTC	4 cut(s)	14, 1533, 2765, 2821
SduI	GDGCHC	7 cut(s)	41, 161, 311, 528, 1860, 2337, 2884
SfcI	CTRYAG	3 cut(s)	1469, 2029, 2517
SinI	GGWCC	3 cut(s)	163, 213, 342
SmiMI	CAYNNNNRTG	2 cut(s)	878, 905
SmlI	CTYRAG	3 cut(s)	527, 980, 2330
SmoI	CTYRAG	3 cut(s)	527, 980, 2330
SphI	GCATGC	1 cut(s)	2434
SseBI	AGGCCT	1 cut(s)	963
SspI	AATATT	1 cut(s)	2109
SspMI	CTAG	7 cut(s)	1112, 1425, 1782, 1907, 1947, 2348, 3197
StuI	AGGCCT	1 cut(s)	963
StyI	CCWWGG	1 cut(s)	270
TaiI	ACGT	5 cut(s)	20, 429, 620, 1157, 2985
TaqI	TCGA	5 cut(s)	101, 687, 2494, 2500, 2797
TaqII	GACCGA	1 cut(s)	1653
TatI	WGTACW	2 cut(s)	142, 734
TauI	GCSGC	3 cut(s)	258, 402, 416
TfiI	GAWTC	6 cut(s)	689, 802, 1011, 1764, 2126, 2578
TscAI	CASTG	5 cut(s)	54, 1478, 2293, 2488, 3111
TseFI	GTSAC	4 cut(s)	755, 1334, 1816, 1921
TseI	GCWGC	6 cut(s)	1226, 1318, 2028, 2702, 2749, 3101
Tsp45I	GTSAC	4 cut(s)	755, 1334, 1816, 1921
TspGWI	ACGGA	1 cut(s)	879
TspRI	CASTG	5 cut(s)	54, 1478, 2293, 2488, 3111
Van91I	CCANNNNNTGG	1 cut(s)	868
Vha464I	CTTAAG	1 cut(s)	980
VneI	GTGCAC	2 cut(s)	37, 2880
VpaK11BI	GGWCC	3 cut(s)	163, 213, 342
XagI	CCTNNNNNAGG	2 cut(s)	968, 1548
XapI	RAATTY	7 cut(s)	93, 920, 985, 1070, 1811, 2943, 3177
XbaI	TCTAGA	1 cut(s)	1424
XceI	RCATGY	3 cut(s)	469, 2434, 2991
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3169
XmiI	GTMKAC	1 cut(s)	612
XspI	CTAG	7 cut(s)	1112, 1425, 1782, 1907, 1947, 2348, 3197
Zsp2I	ATGCAT	3 cut(s)	1987, 2173, 2856

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.