AT5G27400

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011727
Physical Location & Seq
Forward (+)
9676334 .. 9678503
2170 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27400.1

Sequence Viewer

Length: 1110 bp
ATGGCGGCGGAGACGGAGGCAGAATCACAGTACCTTAATGTACTCTCTGCTTTCCTCGCCATCGAACCGGTGGACTCCGTCATCACTCTCGCTAGAGGGTGCACTGGAGGATCAATTACTGAGAACGCCCAGAGATTTTTCTGGGAAATTTGCGTTGAAGCTGCTGCGAATGGTAACGGATCATACGCGAAGAAGTTACTGAAGAAACTCATAAATGAAGTTGAGTTAGAGAATGGTGAAGTATTGGATGAAGTGTATGAAGAGTATGCTCTTTACATGTTAACTTCTAAGGAAGATATATTGGTGAAAGAAAACATAAGGATAACCAAATTCATTTCTTTTCTTTTCCCTGAAGGTTCGTACAAACACCCGAGTTGTCCAAGGTCGAGGAAATTAGTTATTCCGCTGCATTGTTCGTTGAACATGCTTGAAGGAGATACCGGGTGTTCCATCTGGCCATCGAGTCTGTTTCTGTCAGAATTTGTTTTGTCATTTCCAGAGTTATTCGCCAATAAAGCCTGTTTTGAGGTTGGTTCTGGAGTTGGTATGGTCGGGATTTGCCTTGCACATGTAAAGGCCAAAGAGGTGATACTAACAGACGGAGATCTTTTAACTTTGTCTAACATGAAGCTTAATTTGGAGAGAAATCACTTAAACTATGATGACGAGTTTCTCAAACAACCCGGAGAAGCTCAGAGTACACGAGTTAAATGCACTCATCTTCCATGGGAAACTGCATCGGAAAGTGAACTAAGCCAATATCGTCCAGATATTGTTCTAGGCGCAGATGTAATCTATGATCCGTCATGTCTCCCTCATCTACTTCGGGTGCTAGTAGCCCTTTTAAAGAACCCACCCAAAAGAGGAAATGGCTCTCTCGAGACTGAAGACAGAGACACGACACAAGAAGAACACAGTCCCGCGGTGGCTTATATCGCATCTGTGATCCGCAATGTAGACACTTTTAATGTGTGCTTGACACTTGCAGACCAAATGGACCTTTCCATCACAGACGTGACCGCAGAACTTACGCCTCGCTTTGAGCTTTTACCATATATGCATTCGTATGACCGCTCAACCGTTCGGCTCTTCTCCATATCCTCCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAM86 Methyltransf_16

Protein Analysis

369

Amino Acids

41.18

Weight (kDa)

4.88

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 144 - 286 2.4e-18 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011727)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27400
fragaria_vesca FvH4_6g05341 FvH4_6g05341 FvH4_6g05341 FvH4_6g05341 FvH4_6g05341
malus_domestica MD04G1209100.v1.1
prunus_persica Prupe.6G330100_v2.0.a1 Prupe.6G330100_v2.0.a1
pyrus_communis pycom04g18530
rosa_chinensis RchiOBHm_Chr3g0454111
rosa_laevigata RLG00000025482
rosa_multiflora Rmu_co8427317.1_g000001
rosa_roxburghii Rroxscaffold_6G00430900
rosa_rugosa Rorug02G0653200 Rorug02G0653300
rosa_samantha Rh3AG056000 Rh3BG057400 Rh3CG056700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1074
AccI GTMKAC 1 cut(s) 957
AccII CGCG 2 cut(s) 188, 923
AciI CCGC 8 cut(s) 5, 8, 404, 921, 923, 949, 1020, 1072
AclWI GGATC 4 cut(s) 118, 187, 794, 940
AcoI YGGCCR 1 cut(s) 455
AcsI RAATTY 3 cut(s) 147, 329, 479
AcuI CTGAAG 3 cut(s) 221, 372, 906
AfaI GTAC 4 cut(s) 32, 42, 362, 700
AfiI CCNNNNNNNGG 1 cut(s) 863
AflIII ACRYGT 2 cut(s) 276, 568
AgeI ACCGGT 1 cut(s) 67
AgsI TTSAA 3 cut(s) 158, 421, 431
AjiI CACGTC 1 cut(s) 1015
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
AleI CACNNNNGTG 1 cut(s) 1013
AluBI AGCT 4 cut(s) 161, 631, 692, 1045
AluI AGCT 4 cut(s) 161, 631, 692, 1045
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 4 cut(s) 5, 815, 875, 888
Alw44I GTGCAC 1 cut(s) 100
AlwI GGATC 4 cut(s) 118, 187, 794, 940
Ama87I CYCGRG 2 cut(s) 370, 878
AoxI GGCC 2 cut(s) 455, 576
ApaLI GTGCAC 1 cut(s) 100
ApeKI GCWGC 3 cut(s) 161, 164, 406
ApoI RAATTY 3 cut(s) 147, 329, 479
AsiGI ACCGGT 1 cut(s) 67
AspLEI GCGC 1 cut(s) 785
AspS9I GGNCC 1 cut(s) 997
AsuC2I CCSGG 2 cut(s) 442, 684
AsuHPI GGTGA 3 cut(s) 248, 316, 598
AvaI CYCGRG 2 cut(s) 370, 878
AvaII GGWCC 1 cut(s) 997
BaeGI GKGCMC 1 cut(s) 104
BalI TGGCCA 1 cut(s) 457
BauI CACGAG 1 cut(s) 702
BbsI GAAGAC 1 cut(s) 894
Bbv12I GWGCWC 1 cut(s) 104
BbvI GCAGC 3 cut(s) 148, 151, 393
BccI CCATC 4 cut(s) 68, 458, 466, 1013
BcgI CGANNNNNNTGC 2 cut(s) 693, 727
BcnI CCSGG 2 cut(s) 442, 684
BcoDI GTCTC 4 cut(s) 5, 815, 875, 888
BfaI CTAG 3 cut(s) 93, 779, 833
BglII AGATCT 1 cut(s) 604
BisI GCNGC 4 cut(s) 6, 162, 165, 407
BlsI GCNGC 4 cut(s) 7, 163, 166, 408
Bme1390I CCNGG 2 cut(s) 442, 684
Bme18I GGWCC 1 cut(s) 997
BmeT110I CYCGRG 2 cut(s) 370, 878
BmgBI CACGTC 1 cut(s) 1015
BmgT120I GGNCC 1 cut(s) 997
BmrFI CCNGG 2 cut(s) 442, 684
BmsI GCATC 2 cut(s) 746, 947
BpiI GAAGAC 1 cut(s) 894
BpmI CTGGAG 3 cut(s) 126, 558, 1087
BpuMI CCSGG 2 cut(s) 442, 684
BsaJI CCNNGG 3 cut(s) 380, 725, 921
BsaWI WCCGGW 1 cut(s) 67
Bsc4I CCNNNNNNNGG 1 cut(s) 863
Bse118I RCCGGY 1 cut(s) 67
Bse1I ACTGG 1 cut(s) 109
Bse3DI GCAATG 1 cut(s) 958
BseDI CCNNGG 3 cut(s) 380, 725, 921
BseGI GGATG 1 cut(s) 253
BseLI CCNNNNNNNGG 1 cut(s) 863
BseMI GCAATG 1 cut(s) 958
BseMII CTCAG 2 cut(s) 111, 707
BseNI ACTGG 1 cut(s) 109
BseSI GKGCMC 1 cut(s) 104
BseXI GCAGC 3 cut(s) 148, 151, 393
Bsh1236I CGCG 2 cut(s) 188, 923
BshFI GGCC 2 cut(s) 457, 578
BshTI ACCGGT 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 104
BsiHKCI CYCGRG 2 cut(s) 370, 878
BsiSI CCGG 3 cut(s) 68, 441, 684
BslFI GGGAC 1 cut(s) 903
BslI CCNNNNNNNGG 1 cut(s) 863
BsmAI GTCTC 4 cut(s) 5, 815, 875, 888
BsmBI CGTCTC 1 cut(s) 5
BsmFI GGGAC 1 cut(s) 903
BsmI GAATGC 1 cut(s) 1060
BsnI GGCC 2 cut(s) 457, 578
BsoBI CYCGRG 2 cut(s) 370, 878
Bsp1286I GDGCHC 1 cut(s) 104
Bsp143I GATC 5 cut(s) 110, 179, 604, 799, 945
Bsp19I CCATGG 1 cut(s) 725
BspACI CCGC 8 cut(s) 5, 8, 404, 921, 923, 949, 1020, 1072
BspANI GGCC 2 cut(s) 457, 578
BspCNI CTCAG 2 cut(s) 112, 706
BspFNI CGCG 2 cut(s) 188, 923
BspPI GGATC 4 cut(s) 118, 187, 794, 940
BspQI GCTCTTC 1 cut(s) 1094
BsrBI CCGCTC 1 cut(s) 1074
BsrDI GCAATG 1 cut(s) 958
BsrFI RCCGGY 1 cut(s) 67
BsrI ACTGG 1 cut(s) 109
BssAI RCCGGY 1 cut(s) 67
BssECI CCNNGG 3 cut(s) 380, 725, 921
BssMI GATC 5 cut(s) 110, 179, 604, 799, 945
BssSI CACGAG 1 cut(s) 702
BssT1I CCWWGG 2 cut(s) 380, 725
Bst2BI CACGAG 1 cut(s) 702
Bst4CI ACNGT 3 cut(s) 30, 917, 1081
Bst6I CTCTTC 2 cut(s) 255, 1094
BstDEI CTNAG 4 cut(s) 120, 288, 693, 752
BstDSI CCRYGG 2 cut(s) 725, 921
BstF5I GGATG 1 cut(s) 253
BstFNI CGCG 2 cut(s) 188, 923
BstHHI GCGC 1 cut(s) 785
BstKTI GATC 5 cut(s) 113, 182, 607, 802, 948
BstMAI GTCTC 4 cut(s) 5, 815, 875, 888
BstMBI GATC 5 cut(s) 110, 179, 604, 799, 945
BstMWI GCNNNNNNNGC 3 cut(s) 56, 515, 935
BstNSI RCATGY 3 cut(s) 280, 427, 572
BstSCI CCNGG 2 cut(s) 440, 682
BstSLI GKGCMC 1 cut(s) 104
BstUI CGCG 2 cut(s) 188, 923
BstV1I GCAGC 3 cut(s) 148, 151, 393
BstV2I GAAGAC 1 cut(s) 894
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BsuRI GGCC 2 cut(s) 457, 578
BtgI CCRYGG 2 cut(s) 725, 921
BtrI CACGTC 1 cut(s) 1015
BtsCI GGATG 1 cut(s) 253
BtsIMutI CAGTG 1 cut(s) 102
CfoI GCGC 1 cut(s) 785
Cfr10I RCCGGY 1 cut(s) 67
Cfr13I GGNCC 1 cut(s) 997
Cfr42I CCGCGG 1 cut(s) 924
Csp6I GTAC 4 cut(s) 31, 41, 361, 699
CspAI ACCGGT 1 cut(s) 67
CviAII CATG 6 cut(s) 277, 424, 569, 625, 726, 807
CviQI GTAC 4 cut(s) 31, 41, 361, 699
DdeI CTNAG 4 cut(s) 120, 288, 693, 752
DpnI GATC 5 cut(s) 112, 181, 606, 801, 947
DpnII GATC 5 cut(s) 110, 179, 604, 799, 945
DraI TTTAAA 1 cut(s) 846
EaeI YGGCCR 1 cut(s) 455
Eam1104I CTCTTC 2 cut(s) 255, 1094
EarI CTCTTC 2 cut(s) 255, 1094
EciI GGCGGA 1 cut(s) 23
Eco130I CCWWGG 2 cut(s) 380, 725
Eco47I GGWCC 1 cut(s) 997
Eco57I CTGAAG 3 cut(s) 221, 372, 906
Eco88I CYCGRG 2 cut(s) 370, 878
EcoT14I CCWWGG 2 cut(s) 380, 725
EcoT22I ATGCAT 1 cut(s) 1062
ErhI CCWWGG 2 cut(s) 380, 725
Esp3I CGTCTC 1 cut(s) 5
FaeI CATG 6 cut(s) 280, 427, 572, 628, 729, 810
FaqI GGGAC 1 cut(s) 903
FatI CATG 6 cut(s) 276, 423, 568, 624, 725, 806
FauI CCCGC 1 cut(s) 928
FblI GTMKAC 1 cut(s) 957
Fnu4HI GCNGC 4 cut(s) 6, 162, 165, 407
FokI GGATG 1 cut(s) 260
Fsp4HI GCNGC 4 cut(s) 6, 162, 165, 407
FspBI CTAG 3 cut(s) 93, 779, 833
GlaI GCGC 1 cut(s) 784
GluI GCNGC 4 cut(s) 6, 162, 165, 407
GsuI CTGGAG 3 cut(s) 126, 558, 1087
HaeIII GGCC 2 cut(s) 457, 578
HapII CCGG 3 cut(s) 68, 441, 684
HhaI GCGC 1 cut(s) 785
Hin1II CATG 6 cut(s) 280, 427, 572, 628, 729, 810
Hin6I GCGC 1 cut(s) 783
HinP1I GCGC 1 cut(s) 783
HincII GTYRAC 1 cut(s) 282
HindII GTYRAC 1 cut(s) 282
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 3 cut(s) 23, 74, 463
HpaI GTTAAC 1 cut(s) 282
HpaII CCGG 3 cut(s) 68, 441, 684
HphI GGTGA 3 cut(s) 248, 316, 598
Hpy166II GTNNAC 6 cut(s) 73, 102, 282, 701, 749, 958
Hpy188I TCNGA 3 cut(s) 478, 696, 742
Hpy188III TCNNGA 7 cut(s) 497, 537, 553, 767, 878, 880, 1104
Hpy8I GTNNAC 6 cut(s) 73, 102, 282, 701, 749, 958
HpyAV CCTTC 2 cut(s) 347, 425
HpyCH4III ACNGT 3 cut(s) 30, 917, 1081
HpyCH4IV ACGT 1 cut(s) 1014
HpyCH4V TGCA 7 cut(s) 102, 409, 566, 714, 737, 986, 1060
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 515, 935
HpyF3I CTNAG 4 cut(s) 120, 288, 693, 752
HpySE526I ACGT 1 cut(s) 1014
Hsp92II CATG 6 cut(s) 280, 427, 572, 628, 729, 810
HspAI GCGC 1 cut(s) 783
KspAI GTTAAC 1 cut(s) 282
KspI CCGCGG 1 cut(s) 924
Kzo9I GATC 5 cut(s) 110, 179, 604, 799, 945
LguI GCTCTTC 1 cut(s) 1094
Lsp1109I GCAGC 3 cut(s) 148, 151, 393
LweI GCATC 2 cut(s) 746, 947
MaeI CTAG 3 cut(s) 93, 779, 833
MaeII ACGT 1 cut(s) 1014
MaeIII GTNAC 3 cut(s) 173, 195, 1015
MalI GATC 5 cut(s) 112, 181, 606, 801, 947
MbiI CCGCTC 1 cut(s) 1074
MboI GATC 5 cut(s) 110, 179, 604, 799, 945
MboII GAAGA 8 cut(s) 202, 214, 272, 305, 713, 899, 920, 1081
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 1 cut(s) 104
MlsI TGGCCA 1 cut(s) 457
MluCI AATT 6 cut(s) 114, 147, 329, 392, 479, 634
MluNI TGGCCA 1 cut(s) 457
MlyI GAGTC 2 cut(s) 68, 472
Mox20I TGGCCA 1 cut(s) 457
Mph1103I ATGCAT 1 cut(s) 1062
MscI TGGCCA 1 cut(s) 457
MseI TTAA 8 cut(s) 36, 281, 611, 633, 653, 708, 845, 966
MslI CAYNNNNRTG 2 cut(s) 1013, 1065
Msp20I TGGCCA 1 cut(s) 457
MspA1I CMGCKG 2 cut(s) 406, 923
MspI CCGG 3 cut(s) 68, 441, 684
MspR9I CCNGG 2 cut(s) 442, 684
Mva1269I GAATGC 1 cut(s) 1060
MvnI CGCG 2 cut(s) 188, 923
MwoI GCNNNNNNNGC 3 cut(s) 56, 515, 935
NciI CCSGG 2 cut(s) 442, 684
NcoI CCATGG 1 cut(s) 725
NdeII GATC 5 cut(s) 110, 179, 604, 799, 945
NlaIII CATG 6 cut(s) 280, 427, 572, 628, 729, 810
NmuCI GTSAC 1 cut(s) 1015
NsiI ATGCAT 1 cut(s) 1062
NspI RCATGY 3 cut(s) 280, 427, 572
OliI CACNNNNGTG 1 cut(s) 1013
PaeR7I CTCGAG 1 cut(s) 878
PciI ACATGT 2 cut(s) 276, 568
PciSI GCTCTTC 1 cut(s) 1094
PctI GAATGC 1 cut(s) 1060
PfeI GAWTC 1 cut(s) 23
PflFI GACNNNGTC 1 cut(s) 77
PinAI ACCGGT 1 cut(s) 67
PkrI GCNGC 4 cut(s) 7, 163, 166, 408
PleI GAGTC 2 cut(s) 68, 471
PpsI GAGTC 2 cut(s) 68, 471
PscI ACATGT 2 cut(s) 276, 568
PspPI GGNCC 1 cut(s) 997
PsuI RGATCY 1 cut(s) 604
PsyI GACNNNGTC 1 cut(s) 77
RsaI GTAC 4 cut(s) 32, 42, 362, 700
RsaNI GTAC 4 cut(s) 31, 41, 361, 699
RseI CAYNNNNRTG 2 cut(s) 1013, 1065
SacII CCGCGG 1 cut(s) 924
SapI GCTCTTC 1 cut(s) 1094
SaqAI TTAA 8 cut(s) 36, 281, 611, 633, 653, 708, 845, 966
SatI GCNGC 4 cut(s) 6, 162, 165, 407
Sau3AI GATC 5 cut(s) 110, 179, 604, 799, 945
Sau96I GGNCC 1 cut(s) 997
SchI GAGTC 2 cut(s) 68, 472
ScrFI CCNGG 2 cut(s) 442, 684
SduI GDGCHC 1 cut(s) 104
SfaNI GCATC 2 cut(s) 746, 947
Sfr274I CTCGAG 1 cut(s) 878
Sfr303I CCGCGG 1 cut(s) 924
SgrBI CCGCGG 1 cut(s) 924
SinI GGWCC 1 cut(s) 997
SlaI CTCGAG 1 cut(s) 878
SmiMI CAYNNNNRTG 2 cut(s) 1013, 1065
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
Sse9I AATT 6 cut(s) 114, 147, 329, 392, 479, 634
SsiI CCGC 8 cut(s) 5, 8, 404, 921, 923, 949, 1020, 1072
SspMI CTAG 3 cut(s) 93, 779, 833
StyD4I CCNGG 2 cut(s) 440, 682
StyI CCWWGG 2 cut(s) 380, 725
TaaI ACNGT 3 cut(s) 30, 917, 1081
TaiI ACGT 1 cut(s) 1017
TaqI TCGA 4 cut(s) 63, 386, 461, 879
TasI AATT 6 cut(s) 114, 147, 329, 392, 479, 634
TatI WGTACW 2 cut(s) 40, 698
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 8 cut(s) 36, 281, 611, 633, 653, 708, 845, 966
Tru9I TTAA 8 cut(s) 36, 281, 611, 633, 653, 708, 845, 966
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 1 cut(s) 1015
TseI GCWGC 3 cut(s) 161, 164, 406
Tsp45I GTSAC 1 cut(s) 1015
TspDTI ATGAA 5 cut(s) 231, 264, 273, 322, 641
TspGWI ACGGA 5 cut(s) 29, 67, 192, 615, 792
TspRI CASTG 1 cut(s) 109
Tth111I GACNNNGTC 1 cut(s) 77
VneI GTGCAC 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 997
XapI RAATTY 3 cut(s) 147, 329, 479
XceI RCATGY 3 cut(s) 280, 427, 572
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XhoI CTCGAG 1 cut(s) 878
XmiI GTMKAC 1 cut(s) 957
XspI CTAG 3 cut(s) 93, 779, 833
Zsp2I ATGCAT 1 cut(s) 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.