MD04G1209100.v1.1

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011727
Physical Location & Seq
Reverse (-)
29440015 .. 29443488
3474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1209100.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGACGGCCACGGCGGAGCACGACCTCCTCCATCCTTCAACCCCTCCGTGTCTCCACTTGCTCTCAGCTTTCCTCGCCATGGAGCCCACCGACTCCCTCATCGCCTTGGCCCGGGAATGCGGCGGAGGGCGGGTTACTGAGAGAGTGCAGAGGTTCATTTGGGATCATTGCATGGGTAAAAATGCTGGGAAATTACACGCACCGTACCTGAAGAAATTTCTGAAGAAGCTTATTGCCGAAGTTGAGTTGAACTGCGGAGATGTGTTGGATGAGCTATACGAGCATTACGCCCATTTCATGACTTCCTTGAAGGACAGTGATCTTGAAAGAGACAATGCAAGGGTCTTCAAATCCATTTCGTTTCTGTTTCCCGATGGTTGTTTGGAACTTACAAGTTGTCCTGAGTCGCGGAAGCTGATGGTTCCCCTGCAATGTTCGCTCAACATGCTTGAAGGAGATACCGGGTGTTCAGTTTGGCCGTCAAGTCTTTATTTATCAGAGTGTATACTTTCGTTTCCCGACTTATTTACTAATAAGTCGTGCTTTGAGGTTGGCTCGGGTGTTGGTTTGGTTGGTATCTGTCTTGCCCATGTCAAGGCATCGAAGGTGATCCTAAGTGATGGTGACCTGTCAACTTTAGCAAACATGATGGTCAATCTGGAATTGAACCACATTAGCGTTGAAACTAATATGTCAGAAACTACCGAAGATCCGTATATGGTAAAAACCGTAAAATGCCTTCATCTACCTTGGGAGTCTGTCTCAGAGAGTGAGCTTCAGAACCTCAAGCCAGACATAATTTTGGGTGCAGACGTAATTTATGATCCCATATGCCTCCCTCATCTTGTTCGAATACTTGCTTTACTTCTAAACCAGATGAAATTGGAAAGTAAAGGTGCTGATGGTAGCGTCAATGGATATCGTCAAGGGAATGGCAATGGCGATAAATTTGATGCTTTCCTAGCCAAAAGAACAGATAACAGTGCCACAAATGCTGGATCAAAGAAAGGTTCGATGGCTTTTATCGCTACTGTCATCCGCACTATTGACACATTCGAAAAATTTTGTGCTTTGTTGGACTCGGCTAACCTTATCATCAAAGACGTAACTGAAACACTTCGGCCTTCGAATTTGCTTCCGTACATGCAGTCATATGATCGAACCGCTGTTCGGCTGTTTGCCATCTCATGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAM86 Methyltransf_16

Protein Analysis

399

Amino Acids

43.94

Weight (kDa)

5.51

Isoelectric Point (pI)

35.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 150 - 292 2.6e-15 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011727)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27400
fragaria_vesca FvH4_6g05341 FvH4_6g05341 FvH4_6g05341 FvH4_6g05341 FvH4_6g05341
malus_domestica MD04G1209100.v1.1
prunus_persica Prupe.6G330100_v2.0.a1 Prupe.6G330100_v2.0.a1
pyrus_communis pycom04g18530
rosa_chinensis RchiOBHm_Chr3g0454111
rosa_laevigata RLG00000025482
rosa_multiflora Rmu_co8427317.1_g000001
rosa_roxburghii Rroxscaffold_6G00430900
rosa_rugosa Rorug02G0653200 Rorug02G0653300
rosa_samantha Rh3AG056000 Rh3BG057400 Rh3CG056700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 505
AccII CGCG 1 cut(s) 409
AciI CCGC 8 cut(s) 14, 120, 123, 130, 255, 409, 1039, 1164
AclWI GGATC 5 cut(s) 171, 604, 704, 818, 1006
AcoI YGGCCR 2 cut(s) 6, 476
AcsI RAATTY 4 cut(s) 215, 947, 1061, 1129
AcuI CTGAAG 3 cut(s) 230, 242, 761
AfaI GTAC 2 cut(s) 206, 1142
AfiI CCNNNNNNNGG 4 cut(s) 79, 111, 595, 1170
AgsI TTSAA 8 cut(s) 39, 250, 310, 326, 349, 452, 667, 683
AluBI AGCT 5 cut(s) 68, 229, 274, 415, 775
AluI AGCT 5 cut(s) 68, 229, 274, 415, 775
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 3 cut(s) 56, 324, 766
AlwI GGATC 5 cut(s) 171, 604, 704, 818, 1006
Ama87I CYCGRG 2 cut(s) 111, 556
AoxI GGCC 4 cut(s) 6, 108, 476, 1121
ApoI RAATTY 4 cut(s) 215, 947, 1061, 1129
Asp700I GAANNNNTTC 1 cut(s) 1116
AspS9I GGNCC 1 cut(s) 109
AsuC2I CCSGG 3 cut(s) 112, 113, 463
AsuHPI GGTGA 2 cut(s) 619, 635
AsuII TTCGAA 3 cut(s) 850, 1056, 1127
AvaI CYCGRG 2 cut(s) 111, 556
BanII GRGCYC 1 cut(s) 87
BarI GAAGNNNNNNTAC 2 cut(s) 723, 755
BbsI GAAGAC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 21
BccI CCATC 8 cut(s) 39, 368, 412, 614, 643, 896, 1009, 1190
BceAI ACGGC 3 cut(s) 21, 27, 463
BcnI CCSGG 3 cut(s) 112, 113, 463
BcoDI GTCTC 3 cut(s) 56, 324, 766
BfaI CTAG 1 cut(s) 962
BisI GCNGC 1 cut(s) 121
BlsI GCNGC 1 cut(s) 122
Bme1390I CCNGG 3 cut(s) 112, 113, 463
BmeT110I CYCGRG 2 cut(s) 111, 556
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 2 cut(s) 84, 423
BmrFI CCNGG 3 cut(s) 112, 113, 463
BmsI GCATC 2 cut(s) 608, 943
BpiI GAAGAC 1 cut(s) 337
BplI GAGNNNNNCTC 4 cut(s) 539, 571, 746, 778
Bpu14I TTCGAA 3 cut(s) 850, 1056, 1127
BpuEI CTTGAG 1 cut(s) 770
BpuMI CCSGG 3 cut(s) 112, 113, 463
BsaJI CCNNGG 5 cut(s) 9, 78, 105, 111, 749
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 111, 595, 1170
Bse3DI GCAATG 3 cut(s) 166, 437, 943
BseDI CCNNGG 5 cut(s) 9, 78, 105, 111, 749
BseGI GGATG 3 cut(s) 31, 274, 1035
BseLI CCNNNNNNNGG 4 cut(s) 79, 111, 595, 1170
BseMI GCAATG 3 cut(s) 166, 437, 943
BseMII CTCAG 4 cut(s) 78, 129, 393, 777
BseRI GAGGAG 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 185
BsgI GTGCAG 2 cut(s) 167, 828
Bsh1236I CGCG 1 cut(s) 409
BshFI GGCC 4 cut(s) 8, 110, 478, 1123
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 2 cut(s) 111, 556
BsiSI CCGG 2 cut(s) 112, 462
BslI CCNNNNNNNGG 4 cut(s) 79, 111, 595, 1170
BsmAI GTCTC 3 cut(s) 56, 324, 766
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 4 cut(s) 8, 110, 478, 1123
BsoBI CYCGRG 2 cut(s) 111, 556
Bsp119I TTCGAA 3 cut(s) 850, 1056, 1127
Bsp1286I GDGCHC 2 cut(s) 21, 87
Bsp143I GATC 7 cut(s) 163, 319, 609, 709, 823, 998, 1156
Bsp19I CCATGG 1 cut(s) 78
BspACI CCGC 8 cut(s) 14, 120, 123, 130, 255, 409, 1039, 1164
BspANI GGCC 4 cut(s) 8, 110, 478, 1123
BspCNI CTCAG 4 cut(s) 77, 130, 394, 776
BspFNI CGCG 1 cut(s) 409
BspHI TCATGA 1 cut(s) 297
BspLI GGNNCC 2 cut(s) 84, 423
BspPI GGATC 5 cut(s) 171, 604, 704, 818, 1006
BspT104I TTCGAA 3 cut(s) 850, 1056, 1127
BsrDI GCAATG 3 cut(s) 166, 437, 943
BssECI CCNNGG 5 cut(s) 9, 78, 105, 111, 749
BssMI GATC 7 cut(s) 163, 319, 609, 709, 823, 998, 1156
BssNAI GTATAC 1 cut(s) 506
BssT1I CCWWGG 3 cut(s) 78, 105, 749
Bst1107I GTATAC 1 cut(s) 506
Bst4CI ACNGT 5 cut(s) 204, 317, 730, 983, 1033
BstBI TTCGAA 3 cut(s) 850, 1056, 1127
BstDEI CTNAG 5 cut(s) 64, 138, 402, 614, 763
BstDSI CCRYGG 2 cut(s) 9, 78
BstEII GGTNACC 1 cut(s) 623
BstF5I GGATG 3 cut(s) 31, 274, 1035
BstFNI CGCG 1 cut(s) 409
BstKTI GATC 7 cut(s) 166, 322, 612, 712, 826, 1001, 1159
BstMAI GTCTC 3 cut(s) 56, 324, 766
BstMBI GATC 7 cut(s) 163, 319, 609, 709, 823, 998, 1156
BstMWI GCNNNNNNNGC 7 cut(s) 74, 280, 436, 445, 962, 992, 1025
BstNSI RCATGY 2 cut(s) 448, 1147
BstPI GGTNACC 1 cut(s) 623
BstSCI CCNGG 3 cut(s) 110, 111, 461
BstUI CGCG 1 cut(s) 409
BstV2I GAAGAC 1 cut(s) 337
BstX2I RGATCY 1 cut(s) 709
BstYI RGATCY 1 cut(s) 709
BstZ17I GTATAC 1 cut(s) 506
BsuRI GGCC 4 cut(s) 8, 110, 478, 1123
BtgI CCRYGG 2 cut(s) 9, 78
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 3 cut(s) 31, 274, 1035
BtsIMutI CAGTG 2 cut(s) 322, 988
CciI TCATGA 1 cut(s) 297
Cfr13I GGNCC 1 cut(s) 109
Cfr9I CCCGGG 1 cut(s) 111
CseI GACGC 1 cut(s) 898
Csp6I GTAC 2 cut(s) 205, 1141
CviAII CATG 8 cut(s) 79, 172, 298, 445, 590, 646, 1144, 1188
CviQI GTAC 2 cut(s) 205, 1141
DdeI CTNAG 5 cut(s) 64, 138, 402, 614, 763
DpnI GATC 7 cut(s) 165, 321, 611, 711, 825, 1000, 1158
DpnII GATC 7 cut(s) 163, 319, 609, 709, 823, 998, 1156
EaeI YGGCCR 2 cut(s) 6, 476
EciI GGCGGA 2 cut(s) 29, 138
Eco130I CCWWGG 3 cut(s) 78, 105, 749
Eco24I GRGCYC 1 cut(s) 87
Eco32I GATATC 1 cut(s) 920
Eco57I CTGAAG 3 cut(s) 230, 242, 761
Eco88I CYCGRG 2 cut(s) 111, 556
Eco91I GGTNACC 1 cut(s) 623
EcoO65I GGTNACC 1 cut(s) 623
EcoRV GATATC 1 cut(s) 920
EcoT14I CCWWGG 3 cut(s) 78, 105, 749
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 3 cut(s) 78, 105, 749
FaeI CATG 8 cut(s) 82, 175, 301, 448, 593, 649, 1147, 1191
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FatI CATG 8 cut(s) 78, 171, 297, 444, 589, 645, 1143, 1187
FauI CCCGC 1 cut(s) 123
FauNDI CATATG 2 cut(s) 830, 1153
FblI GTMKAC 1 cut(s) 505
Fnu4HI GCNGC 1 cut(s) 121
FokI GGATG 3 cut(s) 18, 281, 1022
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 1 cut(s) 121
FspBI CTAG 1 cut(s) 962
GluI GCNGC 1 cut(s) 121
GsaI CCCAGC 1 cut(s) 189
HaeIII GGCC 4 cut(s) 8, 110, 478, 1123
HapII CCGG 2 cut(s) 112, 462
HgaI GACGC 1 cut(s) 898
Hin1II CATG 8 cut(s) 82, 175, 301, 448, 593, 649, 1147, 1191
HincII GTYRAC 1 cut(s) 633
HindII GTYRAC 1 cut(s) 633
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 4 cut(s) 92, 404, 755, 1079
HpaII CCGG 2 cut(s) 112, 462
HphI GGTGA 2 cut(s) 619, 635
Hpy166II GTNNAC 2 cut(s) 506, 633
Hpy188I TCNGA 5 cut(s) 222, 499, 697, 766, 780
Hpy188III TCNNGA 6 cut(s) 298, 323, 371, 401, 518, 659
Hpy8I GTNNAC 2 cut(s) 506, 633
HpyAV CCTTC 6 cut(s) 45, 304, 446, 598, 749, 1134
HpyCH4III ACNGT 5 cut(s) 204, 317, 730, 983, 1033
HpyCH4IV ACGT 2 cut(s) 813, 1104
HpyCH4V TGCA 6 cut(s) 148, 171, 338, 430, 809, 1147
HpyF10VI GCNNNNNNNGC 7 cut(s) 74, 280, 436, 445, 962, 992, 1025
HpyF3I CTNAG 5 cut(s) 64, 138, 402, 614, 763
HpySE526I ACGT 2 cut(s) 813, 1104
Hsp92II CATG 8 cut(s) 82, 175, 301, 448, 593, 649, 1147, 1191
Kzo9I GATC 7 cut(s) 163, 319, 609, 709, 823, 998, 1156
LmnI GCTCC 2 cut(s) 16, 82
LweI GCATC 2 cut(s) 608, 943
MaeI CTAG 1 cut(s) 962
MaeII ACGT 2 cut(s) 813, 1104
MaeIII GTNAC 3 cut(s) 133, 623, 1105
MalI GATC 7 cut(s) 165, 321, 611, 711, 825, 1000, 1158
MboI GATC 7 cut(s) 163, 319, 609, 709, 823, 998, 1156
MboII GAAGA 4 cut(s) 223, 235, 337, 719
MflI RGATCY 1 cut(s) 709
MhlI GDGCHC 2 cut(s) 21, 87
MluCI AATT 9 cut(s) 191, 215, 662, 798, 816, 881, 947, 1061, 1129
MlyI GAGTC 4 cut(s) 86, 413, 764, 1073
MmeI TCCRAC 2 cut(s) 246, 1056
MroXI GAANNNNTTC 1 cut(s) 1116
MspA1I CMGCKG 1 cut(s) 1166
MspI CCGG 2 cut(s) 112, 462
MspR9I CCNGG 3 cut(s) 112, 113, 463
Mva1269I GAATGC 1 cut(s) 122
MvnI CGCG 1 cut(s) 409
MwoI GCNNNNNNNGC 7 cut(s) 74, 280, 436, 445, 962, 992, 1025
NciI CCSGG 3 cut(s) 112, 113, 463
NcoI CCATGG 1 cut(s) 78
NdeI CATATG 2 cut(s) 830, 1153
NdeII GATC 7 cut(s) 163, 319, 609, 709, 823, 998, 1156
NlaIII CATG 8 cut(s) 82, 175, 301, 448, 593, 649, 1147, 1191
NlaIV GGNNCC 2 cut(s) 84, 423
NmeAIII GCCGAG 1 cut(s) 1061
NmuCI GTSAC 1 cut(s) 623
NspI RCATGY 2 cut(s) 448, 1147
NspV TTCGAA 3 cut(s) 850, 1056, 1127
PagI TCATGA 1 cut(s) 297
PctI GAATGC 1 cut(s) 122
PdmI GAANNNNTTC 1 cut(s) 1116
PkrI GCNGC 1 cut(s) 122
PleI GAGTC 4 cut(s) 86, 412, 763, 1073
PpsI GAGTC 4 cut(s) 86, 412, 763, 1073
PspEI GGTNACC 1 cut(s) 623
PspFI CCCAGC 1 cut(s) 185
PspN4I GGNNCC 2 cut(s) 84, 423
PspPI GGNCC 1 cut(s) 109
PsuI RGATCY 1 cut(s) 709
RsaI GTAC 2 cut(s) 206, 1142
RsaNI GTAC 2 cut(s) 205, 1141
SatI GCNGC 1 cut(s) 121
Sau3AI GATC 7 cut(s) 163, 319, 609, 709, 823, 998, 1156
Sau96I GGNCC 1 cut(s) 109
SchI GAGTC 4 cut(s) 86, 413, 764, 1073
ScrFI CCNGG 3 cut(s) 112, 113, 463
SduI GDGCHC 2 cut(s) 21, 87
SfaNI GCATC 2 cut(s) 608, 943
SfuI TTCGAA 3 cut(s) 850, 1056, 1127
SmaI CCCGGG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 785
SmoI CTYRAG 1 cut(s) 785
Sse9I AATT 9 cut(s) 191, 215, 662, 798, 816, 881, 947, 1061, 1129
SsiI CCGC 8 cut(s) 14, 120, 123, 130, 255, 409, 1039, 1164
SspMI CTAG 1 cut(s) 962
StyD4I CCNGG 3 cut(s) 110, 111, 461
StyI CCWWGG 3 cut(s) 78, 105, 749
TaaI ACNGT 5 cut(s) 204, 317, 730, 983, 1033
TaiI ACGT 2 cut(s) 816, 1107
TaqI TCGA 6 cut(s) 602, 850, 1013, 1056, 1127, 1159
TasI AATT 9 cut(s) 191, 215, 662, 798, 816, 881, 947, 1061, 1129
TauI GCSGC 1 cut(s) 123
TscAI CASTG 2 cut(s) 322, 988
TseFI GTSAC 1 cut(s) 623
Tsp45I GTSAC 1 cut(s) 623
TspDTI ATGAA 4 cut(s) 145, 286, 731, 893
TspGWI ACGGA 3 cut(s) 36, 702, 1128
TspMI CCCGGG 1 cut(s) 111
TspRI CASTG 2 cut(s) 322, 988
XapI RAATTY 4 cut(s) 215, 947, 1061, 1129
XceI RCATGY 2 cut(s) 448, 1147
XmaI CCCGGG 1 cut(s) 111
XmiI GTMKAC 1 cut(s) 505
XmnI GAANNNNTTC 1 cut(s) 1116
XspI CTAG 1 cut(s) 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.