RLG00000025482

branched-chain-amino-acid aminotransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011727
Physical Location & Seq
Reverse (-)
46013663 .. 46016576
2914 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025482

Sequence Viewer

Length: 1200 bp
ATGGCGGACCAGGAACTCCAGCTGCTCTCAGCTTTCCTCGCTACGGAGCCCACCGACTCTCTGATCGGCCTGGCCAGGGAATGCGGTGGAGGGTCGGTTACTGAGAGAGTTCAGAGGTTTATTTGGGAGCATTGCATAACCAAATATGCGGGGAAACTACATGCGCCTTATCTGAGGAAGTTTCTGAAGAAGCTTATTGCTGAAGTTGAGTTGAACCACGGTGATGTGATTGATGAGTTATACGAACTTTATGCACATTACATGACTACTTTGAAGGAGAATGATTTGGAGAAAGATAATGCAAGGGTCTTCAAGTACATTTCATTTCTTTTTCCTGATGGTTGTCTTGAACTTTCAAGATGTTTGGAATCAAGGAAACTGATGGTTCAGCTGCAATGTTCTCTCAACATGCTTGAAGGAGATACTGGGTGTTCAGTTTGGCCATCAAGTCTTCTTTTGTCAGAGTGCATACTTTCATTTCCAGAATTGTTCTCTAATAAGTCATGCTTTGAGGTCGGTTCGGGTGTTGGTTTGGTTGGCATCTGTCTTGCCCATGTGAAAGCATCCAAGGTCATACTAAGTGATGGTGACCTGTCAACTTTAGCAAACATGAAGCTTAATCTGGAACTGAACCACTTGAGCGTTGAAACTGATATGTCAGAAACTACTGAAGATCCAAATATGGTAAAATGCATTCATCTGCCATGGGAATCTGTGTCAGAGAAGGAGCTTCAAAACCACAAGCCAGATATAATTTTGGGTGCAGATGTAATATACGATCCCGTATGCCTCCCACATCTTGTTCGAGTACTTACCCTTCTTTTAAACCAGGCAAAACCGTATCCTGACCAGTGCAGTGGTAATCATTCGGGCTTTTCATTGGGAAGTAAATGTAGTAGTGATGCTAAAGTCAATGTAACTCATCAAGCAAATGCTAACAATGGTGATGAATCCGATGCTTCTCTATGCAAAAGAACAGACAGTGGTGCCTCAAATGTTGGATTGAAGAAGCGTCCGACAGCTTTAATTGCTTCTGTCATTCGCAATGTCGACACATTCAATAACTTTCTCGCTTTGCTAGACGAGGCCAACCTTACCATCGAAGACCTAACTGAATCGCTTAGACCTCTAAATTTTCTTCCTTACATGAAATCATATGATCCCAGCACTATACGACTGTTTTCCATCTCACGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_4 Methyltransf_16

Protein Analysis

400

Amino Acids

44.28

Weight (kDa)

5.29

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 136 - 277 1.6e-18 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011727)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27400
fragaria_vesca FvH4_6g05341 FvH4_6g05341 FvH4_6g05341 FvH4_6g05341 FvH4_6g05341
malus_domestica MD04G1209100.v1.1
prunus_persica Prupe.6G330100_v2.0.a1 Prupe.6G330100_v2.0.a1
pyrus_communis pycom04g18530
rosa_chinensis RchiOBHm_Chr3g0454111
rosa_laevigata RLG00000025482
rosa_multiflora Rmu_co8427317.1_g000001
rosa_roxburghii Rroxscaffold_6G00430900
rosa_rugosa Rorug02G0653200 Rorug02G0653300
rosa_samantha Rh3AG056000 Rh3BG057400 Rh3CG056700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 986
AccI GTMKAC 1 cut(s) 1050
AciI CCGC 3 cut(s) 5, 84, 149
AclWI GGATC 3 cut(s) 668, 773, 1154
AcoI YGGCCR 2 cut(s) 72, 440
AcsI RAATTY 1 cut(s) 1132
AcuI CTGAAG 3 cut(s) 206, 222, 690
AfaI GTAC 2 cut(s) 317, 810
AfiI CCNNNNNNNGG 2 cut(s) 43, 76
AjnI CCWGG 4 cut(s) 9, 69, 74, 828
AluBI AGCT 7 cut(s) 22, 32, 193, 391, 616, 730, 1022
AluI AGCT 7 cut(s) 22, 32, 193, 391, 616, 730, 1022
AlwI GGATC 3 cut(s) 668, 773, 1154
AoxI GGCC 4 cut(s) 67, 72, 440, 1086
ApeKI GCWGC 2 cut(s) 22, 391
ApoI RAATTY 1 cut(s) 1132
AspLEI GCGC 1 cut(s) 166
AspS9I GGNCC 1 cut(s) 7
AsuHPI GGTGA 3 cut(s) 233, 599, 956
AvaII GGWCC 1 cut(s) 7
BalI TGGCCA 2 cut(s) 74, 442
BanI GGYRCC 1 cut(s) 986
BanII GRGCYC 1 cut(s) 51
BarI GAAGNNNNNNTAC 2 cut(s) 801, 833
BbsI GAAGAC 3 cut(s) 301, 443, 1110
BbvI GCAGC 2 cut(s) 9, 378
BccI CCATC 6 cut(s) 332, 376, 451, 578, 1106, 1193
BcgI CGANNNNNNTGC 2 cut(s) 233, 267
BciT130I CCWGG 4 cut(s) 11, 71, 76, 830
BciVI GTATCC 1 cut(s) 852
BfaI CTAG 1 cut(s) 1079
BfuI GTATCC 1 cut(s) 852
BisI GCNGC 2 cut(s) 23, 392
BlsI GCNGC 2 cut(s) 24, 393
BmcAI AGTACT 1 cut(s) 810
Bme1390I CCNGG 4 cut(s) 11, 71, 76, 830
Bme18I GGWCC 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 2 cut(s) 48, 988
BmrFI CCNGG 4 cut(s) 11, 71, 76, 830
BmrI ACTGGG 1 cut(s) 435
BmsI GCATC 4 cut(s) 549, 572, 892, 946
BmuI ACTGGG 1 cut(s) 435
BpiI GAAGAC 3 cut(s) 301, 443, 1110
BpuEI CTTGAG 1 cut(s) 658
BsaAI YACGTR 1 cut(s) 1193
BsaJI CCNNGG 4 cut(s) 75, 217, 567, 704
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 76
Bse1I ACTGG 2 cut(s) 430, 850
Bse3DI GCAATG 3 cut(s) 130, 401, 1051
BseBI CCWGG 4 cut(s) 11, 71, 76, 830
BseDI CCNNGG 4 cut(s) 75, 217, 567, 704
BseGI GGATG 1 cut(s) 563
BseLI CCNNNNNNNGG 2 cut(s) 43, 76
BseMI GCAATG 3 cut(s) 130, 401, 1051
BseMII CTCAG 3 cut(s) 42, 93, 164
BseNI ACTGG 2 cut(s) 430, 850
BseXI GCAGC 2 cut(s) 9, 378
BseYI CCCAGC 1 cut(s) 1163
BsgI GTGCAG 2 cut(s) 783, 874
BshFI GGCC 4 cut(s) 69, 74, 442, 1088
BshNI GGYRCC 1 cut(s) 986
BslI CCNNNNNNNGG 2 cut(s) 43, 76
BsmI GAATGC 2 cut(s) 86, 693
BsnI GGCC 4 cut(s) 69, 74, 442, 1088
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 4 cut(s) 63, 673, 778, 1159
Bsp19I CCATGG 1 cut(s) 704
BspACI CCGC 3 cut(s) 5, 84, 149
BspANI GGCC 4 cut(s) 69, 74, 442, 1088
BspCNI CTCAG 3 cut(s) 41, 94, 165
BspLI GGNNCC 2 cut(s) 48, 988
BspPI GGATC 3 cut(s) 668, 773, 1154
BspT107I GGYRCC 1 cut(s) 986
BsrDI GCAATG 3 cut(s) 130, 401, 1051
BsrI ACTGG 2 cut(s) 430, 850
BssECI CCNNGG 4 cut(s) 75, 217, 567, 704
BssMI GATC 4 cut(s) 63, 673, 778, 1159
BssT1I CCWWGG 2 cut(s) 567, 704
Bst2UI CCWGG 4 cut(s) 11, 71, 76, 830
Bst4CI ACNGT 4 cut(s) 221, 840, 983, 1179
BstBAI YACGTR 1 cut(s) 1193
BstDEI CTNAG 5 cut(s) 28, 102, 173, 578, 1121
BstDSI CCRYGG 2 cut(s) 217, 704
BstEII GGTNACC 1 cut(s) 587
BstF5I GGATG 1 cut(s) 563
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 4 cut(s) 66, 676, 781, 1162
BstMBI GATC 4 cut(s) 63, 673, 778, 1159
BstMWI GCNNNNNNNGC 2 cut(s) 38, 1028
BstNI CCWGG 4 cut(s) 11, 71, 76, 830
BstNSI RCATGY 2 cut(s) 164, 412
BstPI GGTNACC 1 cut(s) 587
BstSCI CCNGG 4 cut(s) 9, 69, 74, 828
BstV1I GCAGC 2 cut(s) 9, 378
BstV2I GAAGAC 3 cut(s) 301, 443, 1110
BstX2I RGATCY 1 cut(s) 673
BstXI CCANNNNNNTGG 1 cut(s) 857
BstYI RGATCY 1 cut(s) 673
BsuI GTATCC 1 cut(s) 852
BsuRI GGCC 4 cut(s) 69, 74, 442, 1088
BtgI CCRYGG 2 cut(s) 217, 704
BtsCI GGATG 1 cut(s) 563
BtsI GCAGTG 1 cut(s) 862
BtsIMutI CAGTG 3 cut(s) 857, 862, 988
CfoI GCGC 1 cut(s) 166
Cfr13I GGNCC 1 cut(s) 7
CseI GACGC 1 cut(s) 1001
Csp6I GTAC 2 cut(s) 316, 809
CviAII CATG 8 cut(s) 161, 262, 409, 504, 554, 610, 705, 1147
CviQI GTAC 2 cut(s) 316, 809
DdeI CTNAG 5 cut(s) 28, 102, 173, 578, 1121
DpnI GATC 4 cut(s) 65, 675, 780, 1161
DpnII GATC 4 cut(s) 63, 673, 778, 1159
DraI TTTAAA 1 cut(s) 825
EaeI YGGCCR 2 cut(s) 72, 440
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 567, 704
Eco24I GRGCYC 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 7
Eco57I CTGAAG 3 cut(s) 206, 222, 690
Eco91I GGTNACC 1 cut(s) 587
EcoO65I GGTNACC 1 cut(s) 587
EcoRII CCWGG 4 cut(s) 9, 69, 74, 828
EcoT14I CCWWGG 2 cut(s) 567, 704
EcoT22I ATGCAT 1 cut(s) 695
EcoT38I GRGCYC 1 cut(s) 51
ErhI CCWWGG 2 cut(s) 567, 704
FaeI CATG 8 cut(s) 164, 265, 412, 507, 557, 613, 708, 1150
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FatI CATG 8 cut(s) 160, 261, 408, 503, 553, 609, 704, 1146
FauI CCCGC 1 cut(s) 142
FauNDI CATATG 1 cut(s) 1156
FblI GTMKAC 1 cut(s) 1050
Fnu4HI GCNGC 2 cut(s) 23, 392
FokI GGATG 1 cut(s) 550
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 2 cut(s) 23, 392
FspBI CTAG 1 cut(s) 1079
GlaI GCGC 1 cut(s) 165
GluI GCNGC 2 cut(s) 23, 392
GsaI CCCAGC 1 cut(s) 1167
HaeIII GGCC 4 cut(s) 69, 74, 442, 1088
HgaI GACGC 1 cut(s) 1001
HhaI GCGC 1 cut(s) 166
Hin1II CATG 8 cut(s) 164, 265, 412, 507, 557, 613, 708, 1150
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HincII GTYRAC 2 cut(s) 597, 1051
HindII GTYRAC 2 cut(s) 597, 1051
HindIII AAGCTT 2 cut(s) 191, 614
HinfI GANTC 5 cut(s) 56, 368, 710, 950, 1115
HphI GGTGA 3 cut(s) 233, 599, 956
Hpy166II GTNNAC 2 cut(s) 597, 1051
Hpy188I TCNGA 9 cut(s) 63, 114, 174, 186, 463, 661, 721, 955, 1017
Hpy188III TCNNGA 6 cut(s) 335, 347, 357, 482, 623, 845
Hpy8I GTNNAC 2 cut(s) 597, 1051
HpyAV CCTTC 4 cut(s) 268, 410, 718, 827
HpyCH4III ACNGT 4 cut(s) 221, 840, 983, 1179
HpyCH4IV ACGT 1 cut(s) 1192
HpyCH4V TGCA 9 cut(s) 135, 254, 302, 394, 468, 693, 764, 855, 969
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 1028
HpyF3I CTNAG 5 cut(s) 28, 102, 173, 578, 1121
HpySE526I ACGT 1 cut(s) 1192
Hsp92II CATG 8 cut(s) 164, 265, 412, 507, 557, 613, 708, 1150
HspAI GCGC 1 cut(s) 164
Kzo9I GATC 4 cut(s) 63, 673, 778, 1159
LmnI GCTCC 3 cut(s) 46, 127, 727
Lsp1109I GCAGC 2 cut(s) 9, 378
LweI GCATC 4 cut(s) 549, 572, 892, 946
MaeI CTAG 1 cut(s) 1079
MaeII ACGT 1 cut(s) 1192
MaeIII GTNAC 3 cut(s) 97, 587, 916
MalI GATC 4 cut(s) 65, 675, 780, 1161
MboI GATC 4 cut(s) 63, 673, 778, 1159
MboII GAAGA 7 cut(s) 199, 301, 443, 683, 1018, 1115, 1130
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 1 cut(s) 51
MlsI TGGCCA 2 cut(s) 74, 442
MluCI AATT 4 cut(s) 485, 753, 1026, 1132
MluNI TGGCCA 2 cut(s) 74, 442
MlyI GAGTC 1 cut(s) 50
MmeI TCCRAC 2 cut(s) 979, 1040
MnlI CCTC 9 cut(s) 47, 83, 108, 168, 505, 800, 1000, 1078, 1137
Mox20I TGGCCA 2 cut(s) 74, 442
Mph1103I ATGCAT 1 cut(s) 695
MscI TGGCCA 2 cut(s) 74, 442
MseI TTAA 3 cut(s) 618, 824, 1025
MslI CAYNNNNRTG 1 cut(s) 222
Msp20I TGGCCA 2 cut(s) 74, 442
MspA1I CMGCKG 2 cut(s) 22, 391
MspR9I CCNGG 4 cut(s) 11, 71, 76, 830
Mva1269I GAATGC 2 cut(s) 86, 693
MvaI CCWGG 4 cut(s) 11, 71, 76, 830
MwoI GCNNNNNNNGC 2 cut(s) 38, 1028
NcoI CCATGG 1 cut(s) 704
NdeI CATATG 1 cut(s) 1156
NdeII GATC 4 cut(s) 63, 673, 778, 1159
NlaIII CATG 8 cut(s) 164, 265, 412, 507, 557, 613, 708, 1150
NlaIV GGNNCC 2 cut(s) 48, 988
NmuCI GTSAC 1 cut(s) 587
NsiI ATGCAT 1 cut(s) 695
NspI RCATGY 2 cut(s) 164, 412
PctI GAATGC 2 cut(s) 86, 693
PfeI GAWTC 4 cut(s) 368, 710, 950, 1115
PkrI GCNGC 2 cut(s) 24, 393
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
Ppu21I YACGTR 1 cut(s) 1193
Psp6I CCWGG 4 cut(s) 9, 69, 74, 828
PspEI GGTNACC 1 cut(s) 587
PspFI CCCAGC 1 cut(s) 1163
PspGI CCWGG 4 cut(s) 9, 69, 74, 828
PspN4I GGNNCC 2 cut(s) 48, 988
PspPI GGNCC 1 cut(s) 7
PsuI RGATCY 1 cut(s) 673
PvuII CAGCTG 2 cut(s) 22, 391
RsaI GTAC 2 cut(s) 317, 810
RsaNI GTAC 2 cut(s) 316, 809
RseI CAYNNNNRTG 1 cut(s) 222
SalI GTCGAC 1 cut(s) 1049
SaqAI TTAA 3 cut(s) 618, 824, 1025
SatI GCNGC 2 cut(s) 23, 392
Sau3AI GATC 4 cut(s) 63, 673, 778, 1159
Sau96I GGNCC 1 cut(s) 7
ScaI AGTACT 1 cut(s) 810
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 4 cut(s) 11, 71, 76, 830
SduI GDGCHC 1 cut(s) 51
SfaNI GCATC 4 cut(s) 549, 572, 892, 946
SinI GGWCC 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 1 cut(s) 637
SmoI CTYRAG 1 cut(s) 637
Sse9I AATT 4 cut(s) 485, 753, 1026, 1132
SsiI CCGC 3 cut(s) 5, 84, 149
SspMI CTAG 1 cut(s) 1079
StyD4I CCNGG 4 cut(s) 9, 69, 74, 828
StyI CCWWGG 2 cut(s) 567, 704
TaaI ACNGT 4 cut(s) 221, 840, 983, 1179
TaiI ACGT 1 cut(s) 1195
TaqI TCGA 3 cut(s) 805, 1050, 1101
TasI AATT 4 cut(s) 485, 753, 1026, 1132
TatI WGTACW 2 cut(s) 315, 808
TfiI GAWTC 4 cut(s) 368, 710, 950, 1115
Tru1I TTAA 3 cut(s) 618, 824, 1025
Tru9I TTAA 3 cut(s) 618, 824, 1025
TscAI CASTG 3 cut(s) 857, 862, 988
TseFI GTSAC 1 cut(s) 587
TseI GCWGC 2 cut(s) 22, 391
Tsp45I GTSAC 1 cut(s) 587
TspDTI ATGAA 7 cut(s) 312, 465, 626, 686, 867, 963, 1163
TspGWI ACGGA 1 cut(s) 59
TspRI CASTG 3 cut(s) 857, 862, 988
VpaK11BI GGWCC 1 cut(s) 7
XapI RAATTY 1 cut(s) 1132
XceI RCATGY 2 cut(s) 164, 412
XmiI GTMKAC 1 cut(s) 1050
XspI CTAG 1 cut(s) 1079
ZrmI AGTACT 1 cut(s) 810
Zsp2I ATGCAT 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.