pycom04g18530

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011727
Physical Location & Seq
Reverse (-)
20549060 .. 20552040
2981 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g18530.4

Sequence Viewer

Length: 1200 bp
ATGACGGCCACGGCGGAGCACGACCTCCTCCATCCTTCAACCCCTCCGTGTCTCCACTTGCTCTCGGCTTTCCTCGCCATAGAGCCCACCGACTCCCTCATCGCCTTGGCCCGGGAATGCGGCGGAGGAAGGGTTACTGAGAGAGTGCAGAGGTTCATTTGGGATCATTGCATGGGTAAAAATGCTGGGAAATTACACGCACTGTACCTGAAGAAATTTCTGAAGAAGCTTATTGCCGAAGTTGAGTTGAACCGCGGAGATGTGTTGGATGAGCTATACGAGCATTACGCCCATTTCATGACTTCTTTGAAGGACAGTGATCTTGAAAGAGACAATGCAAGGGTCTTCAAATCCATTTCGTTTCTGTTTCCCGATGGTTGTTTGGAACTTACAAGTTGTCCCGAGTCGCGGAAGCTGATGGTTCCCCTGCAATGTTCACTCAACATGCTTGAAGGAGATACCGGGTGTTCAGTTTGGCCGTCAAGTCTTTATCTATCAGAGTGTATACTTTCGTTTCCCAACTTATTTACTAATAAGTCGTGCTTTGAGGTTGGCTCAGGTGTTGGTTTGGTTGGTATCTGTCTTGCCCATGTCAAGGCATCGAAGGTGATCCTAAGTGATGGTGACCTGTCAACTTTAGCAAACATGATGGTCAATCTGGAATTGAACCACATTAGCGTTGAAAATAATATGTCAGAAACTACCGAAGATCCGTATATGGTAAAAACCGTAAAATGCCTTCATCTTCCTTGGGAGTCTGTCTCAGAGAGTGAGCTTCAGAACCTCAAGCCAGACATAATTTTGGGTGCAGACGTAATTTATGATCGATCCATATGCCTCCCTCATCTTGTTCAAATACTTGCTTTACTTCTAAACCAGACGAAATTGGAAAGTAAAGGTGCTGATGGTAGCGTCAATGGATATCATCAAGGGAATGGCAACGGCGATAAATTTGATGCTTTCCTATCCAAAAGAACAGACAACAGTGCCACAAATGCTGGATCAAAGAAAGGTTCGATGGCTTTTATCGCTACTGTCATCCGCACTATTGACACATTCGATAAATTTTGTGCTTTGTTGGACTCGGCTAACCTTATCATCAAAGACGTAACTGAAACACTTCGGCCTTCGAATTTGCTTCCGTACATGCAGTCATATGATCGAACCGCTGTTCGGCTGTTTGCCATCTCATGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAM86 Methyltransf_16

Protein Analysis

400

Amino Acids

44.08

Weight (kDa)

5.66

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011727)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27400
fragaria_vesca FvH4_6g05341 FvH4_6g05341 FvH4_6g05341 FvH4_6g05341 FvH4_6g05341
malus_domestica MD04G1209100.v1.1
prunus_persica Prupe.6G330100_v2.0.a1 Prupe.6G330100_v2.0.a1
pyrus_communis pycom04g18530
rosa_chinensis RchiOBHm_Chr3g0454111
rosa_laevigata RLG00000025482
rosa_multiflora Rmu_co8427317.1_g000001
rosa_roxburghii Rroxscaffold_6G00430900
rosa_rugosa Rorug02G0653200 Rorug02G0653300
rosa_samantha Rh3AG056000 Rh3BG057400 Rh3CG056700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 505
AccII CGCG 2 cut(s) 255, 409
AciI CCGC 8 cut(s) 14, 120, 123, 253, 255, 409, 1042, 1167
AclWI GGATC 5 cut(s) 171, 604, 704, 822, 1009
AcoI YGGCCR 2 cut(s) 6, 476
AcsI RAATTY 4 cut(s) 215, 950, 1064, 1132
AcuI CTGAAG 3 cut(s) 230, 242, 761
AfaI GTAC 2 cut(s) 206, 1145
AfiI CCNNNNNNNGG 4 cut(s) 111, 408, 595, 1173
AgsI TTSAA 9 cut(s) 39, 250, 310, 326, 349, 452, 667, 683, 854
AluBI AGCT 4 cut(s) 229, 274, 415, 775
AluI AGCT 4 cut(s) 229, 274, 415, 775
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 3 cut(s) 56, 324, 766
AlwI GGATC 5 cut(s) 171, 604, 704, 822, 1009
Ama87I CYCGRG 2 cut(s) 111, 401
AoxI GGCC 4 cut(s) 6, 108, 476, 1124
ApoI RAATTY 4 cut(s) 215, 950, 1064, 1132
Asp700I GAANNNNTTC 1 cut(s) 1119
AspS9I GGNCC 1 cut(s) 109
AsuC2I CCSGG 3 cut(s) 112, 113, 463
AsuHPI GGTGA 2 cut(s) 619, 635
AsuII TTCGAA 1 cut(s) 1130
AvaI CYCGRG 2 cut(s) 111, 401
BanII GRGCYC 1 cut(s) 87
BarI GAAGNNNNNNTAC 2 cut(s) 723, 755
BbsI GAAGAC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 21
BccI CCATC 8 cut(s) 39, 368, 412, 614, 643, 899, 1012, 1193
BceAI ACGGC 4 cut(s) 21, 27, 463, 958
BcgI CGANNNNNNTGC 2 cut(s) 816, 850
BcnI CCSGG 3 cut(s) 112, 113, 463
BcoDI GTCTC 3 cut(s) 56, 324, 766
BisI GCNGC 1 cut(s) 121
BlsI GCNGC 1 cut(s) 122
Bme1390I CCNGG 3 cut(s) 112, 113, 463
BmeT110I CYCGRG 2 cut(s) 111, 401
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 3 cut(s) 112, 113, 463
BmsI GCATC 2 cut(s) 608, 946
BpiI GAAGAC 1 cut(s) 337
BplI GAGNNNNNCTC 4 cut(s) 539, 571, 746, 778
Bpu10I CCTNAGC 1 cut(s) 556
Bpu14I TTCGAA 1 cut(s) 1130
BpuEI CTTGAG 1 cut(s) 770
BpuMI CCSGG 3 cut(s) 112, 113, 463
Bsa29I ATCGAT 1 cut(s) 826
BsaJI CCNNGG 5 cut(s) 9, 105, 111, 253, 749
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bsc4I CCNNNNNNNGG 4 cut(s) 111, 408, 595, 1173
Bse3DI GCAATG 2 cut(s) 166, 437
BseCI ATCGAT 1 cut(s) 826
BseDI CCNNGG 5 cut(s) 9, 105, 111, 253, 749
BseGI GGATG 3 cut(s) 31, 274, 1038
BseLI CCNNNNNNNGG 4 cut(s) 111, 408, 595, 1173
BseMI GCAATG 2 cut(s) 166, 437
BseMII CTCAG 3 cut(s) 129, 570, 777
BseRI GAGGAG 1 cut(s) 17
BseYI CCCAGC 1 cut(s) 185
BsgI GTGCAG 2 cut(s) 167, 828
Bsh1236I CGCG 2 cut(s) 255, 409
BshFI GGCC 4 cut(s) 8, 110, 478, 1126
BshVI ATCGAT 1 cut(s) 826
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 2 cut(s) 111, 401
BsiSI CCGG 2 cut(s) 112, 462
BslFI GGGAC 1 cut(s) 384
BslI CCNNNNNNNGG 4 cut(s) 111, 408, 595, 1173
BsmAI GTCTC 3 cut(s) 56, 324, 766
BsmFI GGGAC 1 cut(s) 384
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 4 cut(s) 8, 110, 478, 1126
BsoBI CYCGRG 2 cut(s) 111, 401
Bsp119I TTCGAA 1 cut(s) 1130
Bsp1286I GDGCHC 2 cut(s) 21, 87
Bsp143I GATC 8 cut(s) 163, 319, 609, 709, 823, 827, 1001, 1159
BspACI CCGC 8 cut(s) 14, 120, 123, 253, 255, 409, 1042, 1167
BspANI GGCC 4 cut(s) 8, 110, 478, 1126
BspCNI CTCAG 3 cut(s) 130, 569, 776
BspDI ATCGAT 1 cut(s) 826
BspFNI CGCG 2 cut(s) 255, 409
BspHI TCATGA 1 cut(s) 297
BspLI GGNNCC 1 cut(s) 423
BspPI GGATC 5 cut(s) 171, 604, 704, 822, 1009
BspT104I TTCGAA 1 cut(s) 1130
BsrDI GCAATG 2 cut(s) 166, 437
BssECI CCNNGG 5 cut(s) 9, 105, 111, 253, 749
BssMI GATC 8 cut(s) 163, 319, 609, 709, 823, 827, 1001, 1159
BssNAI GTATAC 1 cut(s) 506
BssT1I CCWWGG 2 cut(s) 105, 749
Bst1107I GTATAC 1 cut(s) 506
Bst4CI ACNGT 5 cut(s) 204, 317, 730, 986, 1036
BstBI TTCGAA 1 cut(s) 1130
BstDEI CTNAG 4 cut(s) 138, 556, 614, 763
BstDSI CCRYGG 2 cut(s) 9, 253
BstEII GGTNACC 1 cut(s) 623
BstF5I GGATG 3 cut(s) 31, 274, 1038
BstFNI CGCG 2 cut(s) 255, 409
BstKTI GATC 8 cut(s) 166, 322, 612, 712, 826, 830, 1004, 1162
BstMAI GTCTC 3 cut(s) 56, 324, 766
BstMBI GATC 8 cut(s) 163, 319, 609, 709, 823, 827, 1001, 1159
BstMWI GCNNNNNNNGC 4 cut(s) 74, 280, 995, 1028
BstNSI RCATGY 2 cut(s) 448, 1150
BstPI GGTNACC 1 cut(s) 623
BstSCI CCNGG 3 cut(s) 110, 111, 461
BstUI CGCG 2 cut(s) 255, 409
BstV2I GAAGAC 1 cut(s) 337
BstX2I RGATCY 1 cut(s) 709
BstYI RGATCY 1 cut(s) 709
BstZ17I GTATAC 1 cut(s) 506
Bsu15I ATCGAT 1 cut(s) 826
BsuRI GGCC 4 cut(s) 8, 110, 478, 1126
BsuTUI ATCGAT 1 cut(s) 826
BtgI CCRYGG 2 cut(s) 9, 253
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 3 cut(s) 31, 274, 1038
BtsIMutI CAGTG 3 cut(s) 200, 322, 991
CciI TCATGA 1 cut(s) 297
Cfr13I GGNCC 1 cut(s) 109
Cfr42I CCGCGG 1 cut(s) 256
Cfr9I CCCGGG 1 cut(s) 111
ClaI ATCGAT 1 cut(s) 826
CseI GACGC 1 cut(s) 901
Csp6I GTAC 2 cut(s) 205, 1144
CviAII CATG 7 cut(s) 172, 298, 445, 590, 646, 1147, 1191
CviQI GTAC 2 cut(s) 205, 1144
DdeI CTNAG 4 cut(s) 138, 556, 614, 763
DpnI GATC 8 cut(s) 165, 321, 611, 711, 825, 829, 1003, 1161
DpnII GATC 8 cut(s) 163, 319, 609, 709, 823, 827, 1001, 1159
EaeI YGGCCR 2 cut(s) 6, 476
EciI GGCGGA 2 cut(s) 29, 138
Eco130I CCWWGG 2 cut(s) 105, 749
Eco24I GRGCYC 1 cut(s) 87
Eco32I GATATC 1 cut(s) 923
Eco57I CTGAAG 3 cut(s) 230, 242, 761
Eco88I CYCGRG 2 cut(s) 111, 401
Eco91I GGTNACC 1 cut(s) 623
EcoO65I GGTNACC 1 cut(s) 623
EcoRV GATATC 1 cut(s) 923
EcoT14I CCWWGG 2 cut(s) 105, 749
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 2 cut(s) 105, 749
FaeI CATG 7 cut(s) 175, 301, 448, 593, 649, 1150, 1194
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FaqI GGGAC 1 cut(s) 384
FatI CATG 7 cut(s) 171, 297, 444, 589, 645, 1146, 1190
FauNDI CATATG 2 cut(s) 833, 1156
FblI GTMKAC 1 cut(s) 505
Fnu4HI GCNGC 1 cut(s) 121
FokI GGATG 3 cut(s) 18, 281, 1025
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 1 cut(s) 121
GluI GCNGC 1 cut(s) 121
GsaI CCCAGC 1 cut(s) 189
HaeIII GGCC 4 cut(s) 8, 110, 478, 1126
HapII CCGG 2 cut(s) 112, 462
HgaI GACGC 1 cut(s) 901
Hin1II CATG 7 cut(s) 175, 301, 448, 593, 649, 1150, 1194
HincII GTYRAC 1 cut(s) 633
HindII GTYRAC 1 cut(s) 633
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 4 cut(s) 92, 404, 755, 1082
HpaII CCGG 2 cut(s) 112, 462
HphI GGTGA 2 cut(s) 619, 635
Hpy166II GTNNAC 3 cut(s) 437, 506, 633
Hpy188I TCNGA 5 cut(s) 222, 499, 697, 766, 780
Hpy188III TCNNGA 5 cut(s) 298, 323, 371, 401, 659
Hpy8I GTNNAC 3 cut(s) 437, 506, 633
HpyAV CCTTC 7 cut(s) 45, 123, 304, 446, 598, 749, 1137
HpyCH4III ACNGT 5 cut(s) 204, 317, 730, 986, 1036
HpyCH4IV ACGT 2 cut(s) 813, 1107
HpyCH4V TGCA 6 cut(s) 148, 171, 338, 430, 809, 1150
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 280, 995, 1028
HpyF3I CTNAG 4 cut(s) 138, 556, 614, 763
HpySE526I ACGT 2 cut(s) 813, 1107
Hsp92II CATG 7 cut(s) 175, 301, 448, 593, 649, 1150, 1194
KspI CCGCGG 1 cut(s) 256
Kzo9I GATC 8 cut(s) 163, 319, 609, 709, 823, 827, 1001, 1159
LmnI GCTCC 1 cut(s) 16
LweI GCATC 2 cut(s) 608, 946
MaeII ACGT 2 cut(s) 813, 1107
MaeIII GTNAC 3 cut(s) 133, 623, 1108
MalI GATC 8 cut(s) 165, 321, 611, 711, 825, 829, 1003, 1161
MboI GATC 8 cut(s) 163, 319, 609, 709, 823, 827, 1001, 1159
MboII GAAGA 5 cut(s) 223, 235, 337, 719, 737
MflI RGATCY 1 cut(s) 709
MhlI GDGCHC 2 cut(s) 21, 87
MluCI AATT 9 cut(s) 191, 215, 662, 798, 816, 884, 950, 1064, 1132
MlyI GAGTC 4 cut(s) 86, 413, 764, 1076
MmeI TCCRAC 2 cut(s) 246, 1059
MroXI GAANNNNTTC 1 cut(s) 1119
MspA1I CMGCKG 2 cut(s) 255, 1169
MspI CCGG 2 cut(s) 112, 462
MspR9I CCNGG 3 cut(s) 112, 113, 463
Mva1269I GAATGC 1 cut(s) 122
MvnI CGCG 2 cut(s) 255, 409
MwoI GCNNNNNNNGC 4 cut(s) 74, 280, 995, 1028
NciI CCSGG 3 cut(s) 112, 113, 463
NdeI CATATG 2 cut(s) 833, 1156
NdeII GATC 8 cut(s) 163, 319, 609, 709, 823, 827, 1001, 1159
NlaIII CATG 7 cut(s) 175, 301, 448, 593, 649, 1150, 1194
NlaIV GGNNCC 1 cut(s) 423
NmeAIII GCCGAG 2 cut(s) 44, 1064
NmuCI GTSAC 1 cut(s) 623
NspI RCATGY 2 cut(s) 448, 1150
NspV TTCGAA 1 cut(s) 1130
PagI TCATGA 1 cut(s) 297
PctI GAATGC 1 cut(s) 122
PdmI GAANNNNTTC 1 cut(s) 1119
PkrI GCNGC 1 cut(s) 122
PleI GAGTC 4 cut(s) 86, 412, 763, 1076
PpsI GAGTC 4 cut(s) 86, 412, 763, 1076
PspEI GGTNACC 1 cut(s) 623
PspFI CCCAGC 1 cut(s) 185
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 1 cut(s) 109
PsuI RGATCY 1 cut(s) 709
RsaI GTAC 2 cut(s) 206, 1145
RsaNI GTAC 2 cut(s) 205, 1144
SacII CCGCGG 1 cut(s) 256
SatI GCNGC 1 cut(s) 121
Sau3AI GATC 8 cut(s) 163, 319, 609, 709, 823, 827, 1001, 1159
Sau96I GGNCC 1 cut(s) 109
SchI GAGTC 4 cut(s) 86, 413, 764, 1076
ScrFI CCNGG 3 cut(s) 112, 113, 463
SduI GDGCHC 2 cut(s) 21, 87
SfaNI GCATC 2 cut(s) 608, 946
Sfr303I CCGCGG 1 cut(s) 256
SfuI TTCGAA 1 cut(s) 1130
SgrBI CCGCGG 1 cut(s) 256
SmaI CCCGGG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 785
SmoI CTYRAG 1 cut(s) 785
Sse9I AATT 9 cut(s) 191, 215, 662, 798, 816, 884, 950, 1064, 1132
SsiI CCGC 8 cut(s) 14, 120, 123, 253, 255, 409, 1042, 1167
StyD4I CCNGG 3 cut(s) 110, 111, 461
StyI CCWWGG 2 cut(s) 105, 749
TaaI ACNGT 5 cut(s) 204, 317, 730, 986, 1036
TaiI ACGT 2 cut(s) 816, 1110
TaqI TCGA 6 cut(s) 602, 826, 1016, 1059, 1130, 1162
TasI AATT 9 cut(s) 191, 215, 662, 798, 816, 884, 950, 1064, 1132
TauI GCSGC 1 cut(s) 123
TscAI CASTG 3 cut(s) 207, 322, 991
TseFI GTSAC 1 cut(s) 623
Tsp45I GTSAC 1 cut(s) 623
TspDTI ATGAA 3 cut(s) 145, 286, 731
TspGWI ACGGA 3 cut(s) 36, 702, 1131
TspMI CCCGGG 1 cut(s) 111
TspRI CASTG 3 cut(s) 207, 322, 991
XapI RAATTY 4 cut(s) 215, 950, 1064, 1132
XceI RCATGY 2 cut(s) 448, 1150
XmaI CCCGGG 1 cut(s) 111
XmiI GTMKAC 1 cut(s) 505
XmnI GAANNNNTTC 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.