Prupe.6G330100_v2.0.a1

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0011727
Physical Location & Seq
Reverse (-)
28836110 .. 28839219
3110 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G330100.2

Sequence Viewer

Length: 1221 bp
ATGGCGGACCAGGAGCTCCACCCATCAATCCCTTCATGTCTCCACTTGCTCTCTGCTCTCCTCTCTATGGAGCCCACCGACTCTCTTATCGCCTTGGCCCGGGTATGCGGTGGAGGGTTGGTTACTGAGGAAGTGCAGAGGTTTGTTTGGGACCATTGCATAGCCAAAGATGCTGGGAAGTTGCATGCGCCTTACCTGAAGAAGTTTCTGAAGAAGCTCATTTCTGAAGTTGAGCTGAACCACGGTGATGTGCTGGATGAGCTATACGAACTGTATGCTCATTTCATGGCTTCTTTGAAGGACAGTGATTTTGAAAAAGGAAATGCAAGGGTCTTCAAATGCATTTCATTTCTTTTTCCTGATGGTTGTTCTAAACTTTCGAGTTGTCCCAAGTCGAGGAAACTGATGGCGCCCCTGCAATGTTCACTCAACATGCTTGAAGGAGATACAGGGTGTTCGGTTTGGCCATCAAGTCTTTTTTTATCGGAATGTATACTTTCTTTTCCAGAATTATTTTCTAATAAGTCATGCTTTGAGGTTGGCTCGGGTGTTGGTTTGGTTGGCATCTGTCTTGCCCATGTGAAAGCATCCAAGGTGATACTAAGTGATGGTGACCTGTCAACTTTAGCAAACATGAAGGTCAATCTGGGACTGAACCACTTCAGCGTTGAAACTGATATGTCAGAAACTACTGAAGATCCAAATATGGTTAAATGCCTTCATATGCCCTGGGAATCTGTCTCAGAAAGTGAGCTTCAGAACCTCAAGCCAGACATAATTTTGGGTGCAGATGTAATATATGACCCCGTATGCCTCCCTCATCTTGTTCGAATACTTGCCTTTCTACTAAACCCTAAGAAATTGTATCCTGAGAAGGGGAGTGGTAATCACCTAGGCCTTTCATTGGAAAGTAAATGTACTGATGGTAAGGTCAATGGAACTCATCAAGGGAGTGCTGGTAATGGTGATAAATTCCAAGCTTTTCTACCCAAAAGAACAGAAAACAGTGCCACAAATGCTGGATTGAAGAAAGGCTCAATGGCTTTTATCGCTACTGTGATCCGCAAGATTGAGACATTCAATAAATTCTGTGCTTTGTTAGACTCGGCTAACCTTACCATCAAAGACCTAACTGATTCACTTAGGCCTTTCAATTTGCTTCCCCACATGCACTCATATGATCGAACCGGTGTACGACTATTTGCCATCTCATGCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAM86 Methyltransf_16

Protein Analysis

407

Amino Acids

44.52

Weight (kDa)

6.27

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011727)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G27400
fragaria_vesca FvH4_6g05341 FvH4_6g05341 FvH4_6g05341 FvH4_6g05341 FvH4_6g05341
malus_domestica MD04G1209100.v1.1
prunus_persica Prupe.6G330100_v2.0.a1 Prupe.6G330100_v2.0.a1
pyrus_communis pycom04g18530
rosa_chinensis RchiOBHm_Chr3g0454111
rosa_laevigata RLG00000025482
rosa_multiflora Rmu_co8427317.1_g000001
rosa_roxburghii Rroxscaffold_6G00430900
rosa_rugosa Rorug02G0653200 Rorug02G0653300
rosa_samantha Rh3AG056000 Rh3BG057400 Rh3CG056700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 409
AccI GTMKAC 1 cut(s) 493
AciI CCGC 3 cut(s) 5, 108, 1063
AclWI GGATC 2 cut(s) 692, 1054
AcoI YGGCCR 1 cut(s) 464
AcsI RAATTY 2 cut(s) 971, 1085
AcuI CTGAAG 6 cut(s) 218, 230, 246, 646, 714, 740
AcyI GRCGYC 1 cut(s) 410
AfaI GTAC 2 cut(s) 919, 1194
AfiI CCNNNNNNNGG 5 cut(s) 67, 99, 396, 875, 904
AgeI ACCGGT 1 cut(s) 1187
AgsI TTSAA 8 cut(s) 298, 314, 337, 440, 671, 1027, 1081, 1153
AjnI CCWGG 2 cut(s) 9, 728
AluBI AGCT 6 cut(s) 16, 217, 235, 262, 754, 980
AluI AGCT 6 cut(s) 16, 217, 235, 262, 754, 980
Alw21I GWGCWC 1 cut(s) 18
Alw26I GTCTC 3 cut(s) 44, 745, 1067
AlwI GGATC 2 cut(s) 692, 1054
Ama87I CYCGRG 2 cut(s) 99, 544
AoxI GGCC 4 cut(s) 96, 464, 895, 1145
ApoI RAATTY 2 cut(s) 971, 1085
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
AsiGI ACCGGT 1 cut(s) 1187
Asp700I GAANNNNTTC 1 cut(s) 659
AspA2I CCTAGG 1 cut(s) 892
AspLEI GCGC 2 cut(s) 190, 412
AspS9I GGNCC 3 cut(s) 7, 97, 151
AsuC2I CCSGG 2 cut(s) 100, 101
AsuHPI GGTGA 5 cut(s) 257, 607, 623, 881, 977
AsuII TTCGAA 1 cut(s) 829
AvaI CYCGRG 2 cut(s) 99, 544
AvaII GGWCC 2 cut(s) 7, 151
AvrII CCTAGG 1 cut(s) 892
BalI TGGCCA 1 cut(s) 466
BanI GGYRCC 1 cut(s) 409
BanII GRGCYC 2 cut(s) 18, 75
BbsI GAAGAC 1 cut(s) 325
Bbv12I GWGCWC 1 cut(s) 18
BccI CCATC 8 cut(s) 31, 356, 400, 475, 602, 917, 1127, 1214
BcgI CGANNNNNNTGC 2 cut(s) 257, 291
BciT130I CCWGG 2 cut(s) 11, 730
BciVI GTATCC 1 cut(s) 876
BcnI CCSGG 2 cut(s) 100, 101
BcoDI GTCTC 3 cut(s) 44, 745, 1067
BfaI CTAG 1 cut(s) 893
BfoI RGCGCY 1 cut(s) 413
BfuI GTATCC 1 cut(s) 876
BlnI CCTAGG 1 cut(s) 892
Bme1390I CCNGG 4 cut(s) 11, 100, 101, 730
Bme18I GGWCC 2 cut(s) 7, 151
BmeT110I CYCGRG 2 cut(s) 99, 544
BmgT120I GGNCC 3 cut(s) 7, 97, 151
BmiI GGNNCC 3 cut(s) 72, 152, 411
BmrFI CCNGG 4 cut(s) 11, 100, 101, 730
BmsI GCATC 3 cut(s) 160, 573, 596
BpiI GAAGAC 1 cut(s) 325
BplI GAGNNNNNCTC 2 cut(s) 527, 559
Bpu14I TTCGAA 1 cut(s) 829
BpuEI CTTGAG 1 cut(s) 749
BpuMI CCSGG 2 cut(s) 100, 101
BsaHI GRCGYC 1 cut(s) 410
BsaJI CCNNGG 7 cut(s) 93, 99, 241, 591, 728, 729, 892
BsaWI WCCGGW 1 cut(s) 1187
BsaXI ACNNNNNCTCC 4 cut(s) 105, 135, 943, 973
Bsc4I CCNNNNNNNGG 5 cut(s) 67, 99, 396, 875, 904
Bse118I RCCGGY 1 cut(s) 1187
Bse3DI GCAATG 2 cut(s) 154, 425
BseBI CCWGG 2 cut(s) 11, 730
BseDI CCNNGG 7 cut(s) 93, 99, 241, 591, 728, 729, 892
BseGI GGATG 2 cut(s) 262, 587
BseLI CCNNNNNNNGG 5 cut(s) 67, 99, 396, 875, 904
BseMI GCAATG 2 cut(s) 154, 425
BseMII CTCAG 3 cut(s) 117, 756, 861
BseRI GAGGAG 1 cut(s) 50
BseYI CCCAGC 1 cut(s) 173
BsgI GTGCAG 2 cut(s) 155, 807
BshFI GGCC 4 cut(s) 98, 466, 897, 1147
BshNI GGYRCC 1 cut(s) 409
BshTI ACCGGT 1 cut(s) 1187
BsiHKAI GWGCWC 1 cut(s) 18
BsiHKCI CYCGRG 2 cut(s) 99, 544
BsiSI CCGG 2 cut(s) 100, 1188
BslFI GGGAC 3 cut(s) 164, 372, 663
BslI CCNNNNNNNGG 5 cut(s) 67, 99, 396, 875, 904
BsmAI GTCTC 3 cut(s) 44, 745, 1067
BsmFI GGGAC 3 cut(s) 164, 372, 663
BsnI GGCC 4 cut(s) 98, 466, 897, 1147
BsoBI CYCGRG 2 cut(s) 99, 544
Bsp119I TTCGAA 1 cut(s) 829
Bsp1286I GDGCHC 2 cut(s) 18, 75
Bsp143I GATC 3 cut(s) 697, 1059, 1180
BspACI CCGC 3 cut(s) 5, 108, 1063
BspANI GGCC 4 cut(s) 98, 466, 897, 1147
BspCNI CTCAG 3 cut(s) 118, 755, 862
BspLI GGNNCC 3 cut(s) 72, 152, 411
BspPI GGATC 2 cut(s) 692, 1054
BspT104I TTCGAA 1 cut(s) 829
BspT107I GGYRCC 1 cut(s) 409
BsrDI GCAATG 2 cut(s) 154, 425
BsrFI RCCGGY 1 cut(s) 1187
BssAI RCCGGY 1 cut(s) 1187
BssECI CCNNGG 7 cut(s) 93, 99, 241, 591, 728, 729, 892
BssMI GATC 3 cut(s) 697, 1059, 1180
BssNAI GTATAC 1 cut(s) 494
BssNI GRCGYC 1 cut(s) 410
BssT1I CCWWGG 3 cut(s) 93, 591, 892
Bst1107I GTATAC 1 cut(s) 494
Bst2UI CCWGG 2 cut(s) 11, 730
Bst4CI ACNGT 5 cut(s) 245, 273, 305, 1007, 1057
BstACI GRCGYC 1 cut(s) 410
BstBI TTCGAA 1 cut(s) 829
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 6 cut(s) 126, 602, 742, 855, 870, 1142
BstDSI CCRYGG 1 cut(s) 241
BstEII GGTNACC 1 cut(s) 611
BstF5I GGATG 2 cut(s) 262, 587
BstH2I RGCGCY 1 cut(s) 413
BstHHI GCGC 2 cut(s) 190, 412
BstKTI GATC 3 cut(s) 700, 1062, 1183
BstMAI GTCTC 3 cut(s) 44, 745, 1067
BstMBI GATC 3 cut(s) 697, 1059, 1180
BstMWI GCNNNNNNNGC 4 cut(s) 170, 259, 1016, 1049
BstNI CCWGG 2 cut(s) 11, 730
BstNSI RCATGY 3 cut(s) 188, 436, 1171
BstPI GGTNACC 1 cut(s) 611
BstSCI CCNGG 4 cut(s) 9, 98, 99, 728
BstV2I GAAGAC 1 cut(s) 325
BstX2I RGATCY 1 cut(s) 697
BstYI RGATCY 1 cut(s) 697
BstZ17I GTATAC 1 cut(s) 494
BsuI GTATCC 1 cut(s) 876
BsuRI GGCC 4 cut(s) 98, 466, 897, 1147
BtgI CCRYGG 1 cut(s) 241
BtsCI GGATG 2 cut(s) 262, 587
BtsIMutI CAGTG 2 cut(s) 310, 1012
Cac8I GCNNGC 1 cut(s) 186
CfoI GCGC 2 cut(s) 190, 412
Cfr10I RCCGGY 1 cut(s) 1187
Cfr13I GGNCC 3 cut(s) 7, 97, 151
Cfr9I CCCGGG 1 cut(s) 99
Csp6I GTAC 2 cut(s) 918, 1193
CspAI ACCGGT 1 cut(s) 1187
CviAII CATG 9 cut(s) 36, 185, 286, 433, 528, 578, 634, 1168, 1212
CviQI GTAC 2 cut(s) 918, 1193
DdeI CTNAG 6 cut(s) 126, 602, 742, 855, 870, 1142
DinI GGCGCC 1 cut(s) 411
DpnI GATC 3 cut(s) 699, 1061, 1182
DpnII GATC 3 cut(s) 697, 1059, 1180
EaeI YGGCCR 1 cut(s) 464
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 16
Eco130I CCWWGG 3 cut(s) 93, 591, 892
Eco147I AGGCCT 2 cut(s) 897, 1147
Eco24I GRGCYC 2 cut(s) 18, 75
Eco47I GGWCC 2 cut(s) 7, 151
Eco53kI GAGCTC 1 cut(s) 16
Eco57I CTGAAG 6 cut(s) 218, 230, 246, 646, 714, 740
Eco88I CYCGRG 2 cut(s) 99, 544
Eco91I GGTNACC 1 cut(s) 611
EcoICRI GAGCTC 1 cut(s) 16
EcoO65I GGTNACC 1 cut(s) 611
EcoRII CCWGG 2 cut(s) 9, 728
EcoT14I CCWWGG 3 cut(s) 93, 591, 892
EcoT22I ATGCAT 1 cut(s) 344
EcoT38I GRGCYC 2 cut(s) 18, 75
EgeI GGCGCC 1 cut(s) 411
EheI GGCGCC 1 cut(s) 411
ErhI CCWWGG 3 cut(s) 93, 591, 892
FaeI CATG 9 cut(s) 39, 188, 289, 436, 531, 581, 637, 1171, 1215
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FaqI GGGAC 3 cut(s) 164, 372, 663
FatI CATG 9 cut(s) 35, 184, 285, 432, 527, 577, 633, 1167, 1211
FauNDI CATATG 2 cut(s) 723, 1177
FblI GTMKAC 1 cut(s) 493
FokI GGATG 2 cut(s) 269, 574
FriOI GRGCYC 2 cut(s) 18, 75
FspBI CTAG 1 cut(s) 893
GlaI GCGC 2 cut(s) 189, 411
GsaI CCCAGC 1 cut(s) 177
HaeII RGCGCY 1 cut(s) 413
HaeIII GGCC 4 cut(s) 98, 466, 897, 1147
HapII CCGG 2 cut(s) 100, 1188
HhaI GCGC 2 cut(s) 190, 412
Hin1I GRCGYC 1 cut(s) 410
Hin1II CATG 9 cut(s) 39, 188, 289, 436, 531, 581, 637, 1171, 1215
Hin6I GCGC 2 cut(s) 188, 410
HinP1I GCGC 2 cut(s) 188, 410
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HindIII AAGCTT 1 cut(s) 978
HinfI GANTC 4 cut(s) 80, 734, 1103, 1136
HpaII CCGG 2 cut(s) 100, 1188
HphI GGTGA 5 cut(s) 257, 607, 623, 881, 977
Hpy166II GTNNAC 4 cut(s) 425, 494, 621, 1193
Hpy188I TCNGA 6 cut(s) 210, 226, 487, 685, 745, 759
Hpy188III TCNNGA 3 cut(s) 359, 506, 869
Hpy8I GTNNAC 4 cut(s) 425, 494, 621, 1193
HpyAV CCTTC 6 cut(s) 42, 292, 434, 631, 728, 868
HpyCH4III ACNGT 5 cut(s) 245, 273, 305, 1007, 1057
HpyCH4V TGCA 9 cut(s) 136, 159, 184, 326, 342, 418, 788, 1171, 1215
HpyF10VI GCNNNNNNNGC 4 cut(s) 170, 259, 1016, 1049
HpyF3I CTNAG 6 cut(s) 126, 602, 742, 855, 870, 1142
Hsp92I GRCGYC 1 cut(s) 410
Hsp92II CATG 9 cut(s) 39, 188, 289, 436, 531, 581, 637, 1171, 1215
HspAI GCGC 2 cut(s) 188, 410
KasI GGCGCC 1 cut(s) 409
Kzo9I GATC 3 cut(s) 697, 1059, 1180
LmnI GCTCC 3 cut(s) 13, 21, 70
LweI GCATC 3 cut(s) 160, 573, 596
MaeI CTAG 1 cut(s) 893
MaeIII GTNAC 2 cut(s) 121, 611
MalI GATC 3 cut(s) 699, 1061, 1182
MboI GATC 3 cut(s) 697, 1059, 1180
MboII GAAGA 5 cut(s) 211, 223, 325, 707, 1039
MflI RGATCY 1 cut(s) 697
MhlI GDGCHC 2 cut(s) 18, 75
MlsI TGGCCA 1 cut(s) 466
MluCI AATT 6 cut(s) 509, 777, 860, 971, 1085, 1153
MluNI TGGCCA 1 cut(s) 466
Mly113I GGCGCC 1 cut(s) 410
MlyI GAGTC 2 cut(s) 74, 1097
MnlI CCTC 9 cut(s) 71, 107, 121, 132, 390, 529, 773, 824, 828
Mox20I TGGCCA 1 cut(s) 466
Mph1103I ATGCAT 1 cut(s) 344
MroXI GAANNNNTTC 1 cut(s) 659
MscI TGGCCA 1 cut(s) 466
MseI TTAA 1 cut(s) 711
MslI CAYNNNNRTG 2 cut(s) 246, 1176
Msp20I TGGCCA 1 cut(s) 466
MspI CCGG 2 cut(s) 100, 1188
MspR9I CCNGG 4 cut(s) 11, 100, 101, 730
MvaI CCWGG 2 cut(s) 11, 730
MwoI GCNNNNNNNGC 4 cut(s) 170, 259, 1016, 1049
NarI GGCGCC 1 cut(s) 410
NciI CCSGG 2 cut(s) 100, 101
NdeI CATATG 2 cut(s) 723, 1177
NdeII GATC 3 cut(s) 697, 1059, 1180
NlaIII CATG 9 cut(s) 39, 188, 289, 436, 531, 581, 637, 1171, 1215
NlaIV GGNNCC 3 cut(s) 72, 152, 411
NmeAIII GCCGAG 1 cut(s) 1085
NmuCI GTSAC 1 cut(s) 611
NsiI ATGCAT 1 cut(s) 344
NspI RCATGY 3 cut(s) 188, 436, 1171
NspV TTCGAA 1 cut(s) 829
PaeI GCATGC 1 cut(s) 188
PasI CCCWGGG 1 cut(s) 729
PceI AGGCCT 2 cut(s) 897, 1147
PdmI GAANNNNTTC 1 cut(s) 659
PfeI GAWTC 2 cut(s) 734, 1136
PinAI ACCGGT 1 cut(s) 1187
PleI GAGTC 2 cut(s) 74, 1097
PluTI GGCGCC 1 cut(s) 413
PpsI GAGTC 2 cut(s) 74, 1097
Psp124BI GAGCTC 1 cut(s) 18
Psp6I CCWGG 2 cut(s) 9, 728
PspEI GGTNACC 1 cut(s) 611
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 2 cut(s) 9, 728
PspN4I GGNNCC 3 cut(s) 72, 152, 411
PspPI GGNCC 3 cut(s) 7, 97, 151
PsuI RGATCY 1 cut(s) 697
RsaI GTAC 2 cut(s) 919, 1194
RsaNI GTAC 2 cut(s) 918, 1193
RseI CAYNNNNRTG 2 cut(s) 246, 1176
SacI GAGCTC 1 cut(s) 18
SaqAI TTAA 1 cut(s) 711
Sau3AI GATC 3 cut(s) 697, 1059, 1180
Sau96I GGNCC 3 cut(s) 7, 97, 151
SchI GAGTC 2 cut(s) 74, 1097
ScrFI CCNGG 4 cut(s) 11, 100, 101, 730
SduI GDGCHC 2 cut(s) 18, 75
SfaNI GCATC 3 cut(s) 160, 573, 596
SfoI GGCGCC 1 cut(s) 411
SfuI TTCGAA 1 cut(s) 829
SinI GGWCC 2 cut(s) 7, 151
SmaI CCCGGG 1 cut(s) 101
SmiMI CAYNNNNRTG 2 cut(s) 246, 1176
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
SphI GCATGC 1 cut(s) 188
Sse9I AATT 6 cut(s) 509, 777, 860, 971, 1085, 1153
SseBI AGGCCT 2 cut(s) 897, 1147
SsiI CCGC 3 cut(s) 5, 108, 1063
SspDI GGCGCC 1 cut(s) 409
SspMI CTAG 1 cut(s) 893
SstI GAGCTC 1 cut(s) 18
StuI AGGCCT 2 cut(s) 897, 1147
StyD4I CCNGG 4 cut(s) 9, 98, 99, 728
StyI CCWWGG 3 cut(s) 93, 591, 892
TaaI ACNGT 5 cut(s) 245, 273, 305, 1007, 1057
TaqI TCGA 4 cut(s) 380, 395, 829, 1183
TasI AATT 6 cut(s) 509, 777, 860, 971, 1085, 1153
TatI WGTACW 1 cut(s) 917
TfiI GAWTC 2 cut(s) 734, 1136
Tru1I TTAA 1 cut(s) 711
Tru9I TTAA 1 cut(s) 711
TscAI CASTG 2 cut(s) 310, 1012
TseFI GTSAC 1 cut(s) 611
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 6 cut(s) 24, 274, 336, 650, 710, 891
TspMI CCCGGG 1 cut(s) 99
TspRI CASTG 2 cut(s) 310, 1012
VpaK11BI GGWCC 2 cut(s) 7, 151
XapI RAATTY 2 cut(s) 971, 1085
XceI RCATGY 3 cut(s) 188, 436, 1171
XmaI CCCGGG 1 cut(s) 99
XmaJI CCTAGG 1 cut(s) 892
XmiI GTMKAC 1 cut(s) 493
XmnI GAANNNNTTC 1 cut(s) 659
XspI CTAG 1 cut(s) 893
Zsp2I ATGCAT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.