AT5G27560

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009621
Physical Location & Seq
Forward (+)
9731512 .. 9734473
2962 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G27560.4

Sequence Viewer

Length: 1026 bp
ATGGCTTATTCTTCAGTACCAAGCTGTTTCCATGGCTATCTTCTCCATCGTTGTTCGTTCTCCACCACACAATTTTTCTCGGGTTCTCTCTCTTGTGGCTTCTCTGCTTCTACCAATTCAAGACTAAGAACATGTGTCAAATTCGAGAAGTTTCAGGGTGACTCTCCTCTTGAACAGAACACTTCAGTTTCTTCTTCCTCTTCTGTTTCACAGGAACTACCATTAGAAGAAGCTCAAGACCAACTAGAAGAAGAAGAAGATGATGATGACTGTTTGCCTAATGATTTGGAAGGAGCTGTTAGGCAATCAGGTGAAGCAGGTGCTGCGTTTGTGAACTCAGGAGGAAATAGAGCTATTGTGGAGCTTTTGATTCCTCAGCTGCAGTTTTTAGACGATGAAGGTGCACAAGCTGAACTTTGGGACCTTTCGCGTGTTTTCTTGGAGACTCTCATTAAAGAAACAGGTTGTGAGAGAGTTAAAGCTGTATTTCCAGACGCTGGAGCTGCTGCATTGCTAAAATACCGGTGGAAAGATGCAACGTTCGGATTTGCTAGCTTAAGTGACCGGAAACCTGTGGAGAAAGAAGATGAGATCATTGTCATGGTTGTTCCTGATTACCAAATGCTGGAATATGTTGAGAAAATTGCTAATGGGCTTGCTGATGACCCGCCAAGGCCTCTCATTATGTGGAACCCTCGTCTTATAAGCGAGGAAGTCGGGGTTGGGTTTAACGTGAGAAAACTAAGGCGTTACTTCCTAAGTTCATTCACTACGGTTTACTCTATGAGACCTCTGGCTGCTGGTGCTGTTTTCAGGTGTTATCCTGGTAAGTGGAAAGTCTTCTATGATAACAAAGACAGACCAGGCCGATACCTACTCGCTAAAGAACTCATTGGTCGACCTGATGCAGAGGATCTTGAGATAATATATGGGGGAGTAGAAGAGAAATCAGAAGAAGGGCCATCTTTAATGAGTCAAGCTGCTGGAATTTTCTCATCCATTAACAGGTTCATGAAATCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

341

Amino Acids

38.05

Weight (kDa)

4.74

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1995 PF09353 93 - 307 8.1e-53 Domain of unknown function (DUF1995)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 704
AarI CACCTGC 1 cut(s) 308
Acc36I ACCTGC 1 cut(s) 308
AccB7I CCANNNNNTGG 2 cut(s) 497, 625
AccI GTMKAC 1 cut(s) 898
AccII CGCG 1 cut(s) 430
AciI CCGC 1 cut(s) 668
AclI AACGTT 1 cut(s) 539
AclWI GGATC 1 cut(s) 921
AcsI RAATTY 2 cut(s) 140, 987
AcuI CTGAAG 1 cut(s) 168
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 3 cut(s) 497, 625, 1005
AflII CTTAAG 1 cut(s) 556
AflIII ACRYGT 1 cut(s) 131
AgeI ACCGGT 1 cut(s) 522
AgsI TTSAA 2 cut(s) 120, 173
AjnI CCWGG 2 cut(s) 823, 862
AjuI GAANNNNNNNTTGG 2 cut(s) 705, 737
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 2 cut(s) 437, 781
Alw44I GTGCAC 1 cut(s) 402
AlwI GGATC 1 cut(s) 921
AlwNI CAGNNNCTG 2 cut(s) 323, 497
Ama87I CYCGRG 1 cut(s) 79
AoxI GGCC 3 cut(s) 674, 865, 959
ApaLI GTGCAC 1 cut(s) 402
ApeKI GCWGC 6 cut(s) 323, 379, 503, 506, 797, 980
ApoI RAATTY 2 cut(s) 140, 987
AsiGI ACCGGT 1 cut(s) 522
Asp700I GAANNNNTTC 1 cut(s) 839
AspS9I GGNCC 2 cut(s) 421, 959
AsuHPI GGTGA 2 cut(s) 170, 323
AsuNHI GCTAGC 1 cut(s) 551
AvaI CYCGRG 1 cut(s) 79
AvaII GGWCC 1 cut(s) 421
BaeGI GKGCMC 1 cut(s) 406
BbsI GAAGAC 1 cut(s) 832
Bbv12I GWGCWC 1 cut(s) 406
BbvCI CCTCAGC 1 cut(s) 375
BbvI GCAGC 6 cut(s) 310, 366, 490, 493, 784, 967
BccI CCATC 2 cut(s) 54, 970
BcgI CGANNNNNNTGC 2 cut(s) 383, 417
BciT130I CCWGG 2 cut(s) 825, 864
BcoDI GTCTC 2 cut(s) 437, 781
BfaI CTAG 2 cut(s) 245, 552
BfmI CTRYAG 1 cut(s) 380
BfrI CTTAAG 1 cut(s) 556
BfuAI ACCTGC 1 cut(s) 308
BisI GCNGC 6 cut(s) 324, 380, 504, 507, 798, 981
BlsI GCNGC 6 cut(s) 325, 381, 505, 508, 799, 982
Bme1390I CCNGG 2 cut(s) 825, 864
Bme18I GGWCC 1 cut(s) 421
BmeT110I CYCGRG 1 cut(s) 79
BmgT120I GGNCC 2 cut(s) 421, 959
BmiI GGNNCC 2 cut(s) 422, 692
BmrFI CCNGG 2 cut(s) 825, 864
BmsI GCATC 2 cut(s) 523, 895
BmtI GCTAGC 1 cut(s) 555
BpiI GAAGAC 1 cut(s) 832
BpmI CTGGAG 1 cut(s) 519
Bpu10I CCTNAGC 1 cut(s) 375
BpuEI CTTGAG 2 cut(s) 219, 938
BsaI GGTCTC 1 cut(s) 781
BsaJI CCNNGG 2 cut(s) 31, 671
BsaWI WCCGGW 2 cut(s) 522, 564
Bsc4I CCNNNNNNNGG 3 cut(s) 497, 625, 1005
Bse118I RCCGGY 1 cut(s) 522
Bse3DI GCAATG 1 cut(s) 509
BseBI CCWGG 2 cut(s) 825, 864
BseDI CCNNGG 2 cut(s) 31, 671
BseGI GGATG 1 cut(s) 995
BseLI CCNNNNNNNGG 3 cut(s) 497, 625, 1005
BseMI GCAATG 1 cut(s) 509
BseMII CTCAG 2 cut(s) 351, 389
BseRI GAGGAG 1 cut(s) 156
BseSI GKGCMC 1 cut(s) 406
BseXI GCAGC 6 cut(s) 310, 366, 490, 493, 784, 967
Bsh1236I CGCG 1 cut(s) 430
BshFI GGCC 3 cut(s) 676, 867, 961
BshTI ACCGGT 1 cut(s) 522
BsiHKAI GWGCWC 1 cut(s) 406
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 2 cut(s) 523, 565
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 3 cut(s) 497, 625, 1005
BsmAI GTCTC 2 cut(s) 437, 781
BsmFI GGGAC 1 cut(s) 434
BsnI GGCC 3 cut(s) 676, 867, 961
Bso31I GGTCTC 1 cut(s) 781
BsoBI CYCGRG 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 406
Bsp143I GATC 2 cut(s) 591, 913
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 1 cut(s) 668
BspANI GGCC 3 cut(s) 676, 867, 961
BspCNI CTCAG 2 cut(s) 350, 388
BspFNI CGCG 1 cut(s) 430
BspHI TCATGA 1 cut(s) 1011
BspLI GGNNCC 2 cut(s) 422, 692
BspMAI CTGCAG 1 cut(s) 384
BspMI ACCTGC 1 cut(s) 308
BspOI GCTAGC 1 cut(s) 555
BspPI GGATC 1 cut(s) 921
BspTI CTTAAG 1 cut(s) 556
BspTNI GGTCTC 1 cut(s) 781
BsrDI GCAATG 1 cut(s) 509
BsrFI RCCGGY 1 cut(s) 522
BssAI RCCGGY 1 cut(s) 522
BssECI CCNNGG 2 cut(s) 31, 671
BssMI GATC 2 cut(s) 591, 913
BssT1I CCWWGG 2 cut(s) 31, 671
Bst2UI CCWGG 2 cut(s) 825, 864
Bst4CI ACNGT 2 cut(s) 272, 775
Bst6I CTCTTC 2 cut(s) 205, 936
BstAFI CTTAAG 1 cut(s) 556
BstAPI GCANNNNNTGC 1 cut(s) 323
BstC8I GCNNGC 2 cut(s) 553, 657
BstDEI CTNAG 5 cut(s) 125, 337, 375, 743, 758
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 1 cut(s) 995
BstFNI CGCG 1 cut(s) 430
BstKTI GATC 2 cut(s) 594, 916
BstMAI GTCTC 2 cut(s) 437, 781
BstMBI GATC 2 cut(s) 591, 913
BstMWI GCNNNNNNNGC 3 cut(s) 323, 503, 803
BstNI CCWGG 2 cut(s) 825, 864
BstNSI RCATGY 1 cut(s) 135
BstSCI CCNGG 2 cut(s) 823, 862
BstSFI CTRYAG 1 cut(s) 380
BstSLI GKGCMC 1 cut(s) 406
BstUI CGCG 1 cut(s) 430
BstV1I GCAGC 6 cut(s) 310, 366, 490, 493, 784, 967
BstV2I GAAGAC 1 cut(s) 832
BstX2I RGATCY 1 cut(s) 913
BstYI RGATCY 1 cut(s) 913
BsuRI GGCC 3 cut(s) 676, 867, 961
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 1 cut(s) 995
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 2 cut(s) 553, 657
CaiI CAGNNNCTG 2 cut(s) 323, 497
CciI TCATGA 1 cut(s) 1011
Cfr10I RCCGGY 1 cut(s) 522
Cfr13I GGNCC 2 cut(s) 421, 959
CseI GACGC 1 cut(s) 503
Csp6I GTAC 1 cut(s) 17
CspAI ACCGGT 1 cut(s) 522
CviAII CATG 4 cut(s) 32, 132, 601, 1012
CviQI GTAC 1 cut(s) 17
DdeI CTNAG 5 cut(s) 125, 337, 375, 743, 758
DpnI GATC 2 cut(s) 593, 915
DpnII GATC 2 cut(s) 591, 913
Eam1104I CTCTTC 2 cut(s) 205, 936
EarI CTCTTC 2 cut(s) 205, 936
Eco130I CCWWGG 2 cut(s) 31, 671
Eco147I AGGCCT 1 cut(s) 676
Eco31I GGTCTC 1 cut(s) 781
Eco47I GGWCC 1 cut(s) 421
Eco57I CTGAAG 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 79
EcoO109I RGGNCCY 1 cut(s) 421
EcoRII CCWGG 2 cut(s) 823, 862
EcoT14I CCWWGG 2 cut(s) 31, 671
ErhI CCWWGG 2 cut(s) 31, 671
FaeI CATG 4 cut(s) 35, 135, 604, 1015
FalI AAGNNNNNCTT 2 cut(s) 399, 431
FaqI GGGAC 1 cut(s) 434
FatI CATG 4 cut(s) 31, 131, 600, 1011
FauI CCCGC 1 cut(s) 675
FblI GTMKAC 1 cut(s) 898
Fnu4HI GCNGC 6 cut(s) 324, 380, 504, 507, 798, 981
FokI GGATG 1 cut(s) 982
Fsp4HI GCNGC 6 cut(s) 324, 380, 504, 507, 798, 981
FspBI CTAG 2 cut(s) 245, 552
GluI GCNGC 6 cut(s) 324, 380, 504, 507, 798, 981
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 3 cut(s) 676, 867, 961
HapII CCGG 2 cut(s) 523, 565
HgaI GACGC 1 cut(s) 503
Hin1II CATG 4 cut(s) 35, 135, 604, 1015
HincII GTYRAC 1 cut(s) 899
HindII GTYRAC 1 cut(s) 899
HinfI GANTC 4 cut(s) 161, 370, 445, 973
HpaII CCGG 2 cut(s) 523, 565
HphI GGTGA 2 cut(s) 170, 323
Hpy166II GTNNAC 4 cut(s) 334, 404, 778, 899
Hpy188I TCNGA 2 cut(s) 545, 952
Hpy188III TCNNGA 9 cut(s) 120, 145, 170, 236, 339, 491, 611, 917, 1012
Hpy8I GTNNAC 4 cut(s) 334, 404, 778, 899
HpyAV CCTTC 3 cut(s) 284, 392, 950
HpyCH4III ACNGT 2 cut(s) 272, 775
HpyCH4IV ACGT 2 cut(s) 539, 732
HpyCH4V TGCA 5 cut(s) 382, 404, 509, 536, 908
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 503, 803
HpyF3I CTNAG 5 cut(s) 125, 337, 375, 743, 758
HpySE526I ACGT 2 cut(s) 539, 732
Hsp92II CATG 4 cut(s) 35, 135, 604, 1015
Kzo9I GATC 2 cut(s) 591, 913
LmnI GCTCC 3 cut(s) 293, 361, 500
Lsp1109I GCAGC 6 cut(s) 310, 366, 490, 493, 784, 967
LweI GCATC 2 cut(s) 523, 895
MaeI CTAG 2 cut(s) 245, 552
MaeII ACGT 2 cut(s) 539, 732
MaeIII GTNAC 3 cut(s) 158, 560, 749
MalI GATC 2 cut(s) 593, 915
MboI GATC 2 cut(s) 591, 913
MflI RGATCY 1 cut(s) 913
MhlI GDGCHC 1 cut(s) 406
MluCI AATT 5 cut(s) 71, 115, 140, 642, 987
MlyI GAGTC 3 cut(s) 155, 439, 982
MnlI CCTC 9 cut(s) 177, 208, 335, 384, 687, 703, 705, 801, 904
MroXI GAANNNNTTC 1 cut(s) 839
MseI TTAA 6 cut(s) 453, 477, 557, 729, 968, 1002
MslI CAYNNNNRTG 1 cut(s) 599
MspA1I CMGCKG 1 cut(s) 379
MspCI CTTAAG 1 cut(s) 556
MspI CCGG 2 cut(s) 523, 565
MspR9I CCNGG 2 cut(s) 825, 864
MvaI CCWGG 2 cut(s) 825, 864
MvnI CGCG 1 cut(s) 430
MwoI GCNNNNNNNGC 3 cut(s) 323, 503, 803
NcoI CCATGG 1 cut(s) 31
NdeII GATC 2 cut(s) 591, 913
NheI GCTAGC 1 cut(s) 551
NlaIII CATG 4 cut(s) 35, 135, 604, 1015
NlaIV GGNNCC 2 cut(s) 422, 692
NmuCI GTSAC 2 cut(s) 158, 560
NspI RCATGY 1 cut(s) 135
PagI TCATGA 1 cut(s) 1011
PaqCI CACCTGC 1 cut(s) 308
PceI AGGCCT 1 cut(s) 676
PciI ACATGT 1 cut(s) 131
PdmI GAANNNNTTC 1 cut(s) 839
PfeI GAWTC 1 cut(s) 370
PflMI CCANNNNNTGG 2 cut(s) 497, 625
PinAI ACCGGT 1 cut(s) 522
PkrI GCNGC 6 cut(s) 325, 381, 505, 508, 799, 982
PleI GAGTC 3 cut(s) 155, 439, 981
PpsI GAGTC 3 cut(s) 155, 439, 981
PpuMI RGGWCCY 1 cut(s) 421
PscI ACATGT 1 cut(s) 131
PsiI TTATAA 1 cut(s) 704
Psp1406I AACGTT 1 cut(s) 539
Psp5II RGGWCCY 1 cut(s) 421
Psp6I CCWGG 2 cut(s) 823, 862
PspGI CCWGG 2 cut(s) 823, 862
PspN4I GGNNCC 2 cut(s) 422, 692
PspPI GGNCC 2 cut(s) 421, 959
PspPPI RGGWCCY 1 cut(s) 421
PstI CTGCAG 1 cut(s) 384
PstNI CAGNNNCTG 2 cut(s) 323, 497
PsuI RGATCY 1 cut(s) 913
PvuII CAGCTG 1 cut(s) 379
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
RseI CAYNNNNRTG 1 cut(s) 599
SalI GTCGAC 1 cut(s) 897
SaqAI TTAA 6 cut(s) 453, 477, 557, 729, 968, 1002
SatI GCNGC 6 cut(s) 324, 380, 504, 507, 798, 981
Sau3AI GATC 2 cut(s) 591, 913
Sau96I GGNCC 2 cut(s) 421, 959
SchI GAGTC 3 cut(s) 155, 439, 982
ScrFI CCNGG 2 cut(s) 825, 864
SduI GDGCHC 1 cut(s) 406
SfaNI GCATC 2 cut(s) 523, 895
SfcI CTRYAG 1 cut(s) 380
SinI GGWCC 1 cut(s) 421
SmiMI CAYNNNNRTG 1 cut(s) 599
SmlI CTYRAG 3 cut(s) 234, 556, 917
SmoI CTYRAG 3 cut(s) 234, 556, 917
Sse9I AATT 5 cut(s) 71, 115, 140, 642, 987
SseBI AGGCCT 1 cut(s) 676
SsiI CCGC 1 cut(s) 668
SspMI CTAG 2 cut(s) 245, 552
StuI AGGCCT 1 cut(s) 676
StyD4I CCNGG 2 cut(s) 823, 862
StyI CCWWGG 2 cut(s) 31, 671
TaaI ACNGT 2 cut(s) 272, 775
TaiI ACGT 2 cut(s) 542, 735
TaqI TCGA 2 cut(s) 144, 898
TasI AATT 5 cut(s) 71, 115, 140, 642, 987
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 6 cut(s) 453, 477, 557, 729, 968, 1002
Tru9I TTAA 6 cut(s) 453, 477, 557, 729, 968, 1002
TseFI GTSAC 2 cut(s) 158, 560
TseI GCWGC 6 cut(s) 323, 379, 503, 506, 797, 980
Tsp45I GTSAC 2 cut(s) 158, 560
TspDTI ATGAA 3 cut(s) 411, 753, 1000
Van91I CCANNNNNTGG 2 cut(s) 497, 625
Vha464I CTTAAG 1 cut(s) 556
VneI GTGCAC 1 cut(s) 402
VpaK11BI GGWCC 1 cut(s) 421
XapI RAATTY 2 cut(s) 140, 987
XceI RCATGY 1 cut(s) 135
XmiI GTMKAC 1 cut(s) 898
XmnI GAANNNNTTC 1 cut(s) 839
XspI CTAG 2 cut(s) 245, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.