Rw3G004840

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0009621
Physical Location & Seq
Forward (+)
4079382 .. 4083085
3704 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G004840.1

Sequence Viewer

Length: 1038 bp
ATGGCTTCCACGTACCCAAGTTCCCTGTTTTACTTCTGCAGCTCCAGTTCTTCAAAACCCAGTTCAACTTCAGCCACACGCTTGCCCTTGGCTTTTTCTGGGTCTGGCTTTAATGGGTGCAATGGAGTTTCAGTCACCAAAGCTTGGTCTAGTAGTAGCTCAAGAATATGTGCCAAGTTTGAAAAGTTCCAAGCTGAGGAGAATCTTGAAGAGACCAACATGACATCATCTTTACAAGAAGTTGAAGAAGATGGTGAGCAGGAAGAAGATGACAGCTGCTTGCCTTCGGACTTGGAGGGTGCAGTCCGTCAATCGGGTGAAGCAAGTGCATCATTTGTTTCTTCAGGAGGACTGAGAGCCATAGTTGAGCTTTTAATTCCCCAGCTGCAGTTTCTAGATGAAGAAGGGGCACAAGCTGAGCTCTGGGAGTTGTCAAGGGTTTTCCTGGATACCCTTATAGAGGAAACTGGATGTGAGAGAGTTAAAGCTGTATTTCCTGATGCTGGAGCAGCAGCACTACTAAAATATAAGTGGACAGATGCTGCTTTTGGATTCGCCAGCTTAAGTGACCGGAAACCCATAACAAGTGAAGATGAGATTGTGGTTATGATTGTTCCTGATTATCAGATGTTGGAATATGTAGAGAGACTTGCAGCCAGTCTTTCAGATGATCCGCCAAGGCCTCTAATCATGTGGAACCCACGTCTCATCAGTGAGGATGTTGGAGTTGGATTTAATGTACGAAAGTTAAGGCGGTACTTTCTGAGCACTTTTACAACGGTCTACTCAATGAGGCCTATGGAATCCGGTGCTGTATTTAGGTGCTATCCAGGGTCATGGAAGGTATTCTTCGATGATAAGGATAGACCAAACAGATATCTACTTGCCAAAGAGCTCCTGCGCCGTCCTGATGCTGAAGACCTTGAGATGATATTTGGAAATATGGACGAGGATTCAGAGCAGGGCCCGTCTTTGTTTGATAAAGCAGCAGGGATGTTCTCTTCAATGAACCGATTTATGAGGATCATTTCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

345

Amino Acids

38.49

Weight (kDa)

4.57

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1995 PF09353 95 - 309 2.4e-51 Domain of unknown function (DUF1995)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 144
AccI GTMKAC 1 cut(s) 783
AciI CCGC 2 cut(s) 674, 754
AclWI GGATC 2 cut(s) 665, 1031
AcuI CTGAAG 3 cut(s) 54, 327, 936
AfaI GTAC 3 cut(s) 14, 741, 758
AfiI CCNNNNNNNGG 5 cut(s) 144, 196, 313, 460, 503
AflII CTTAAG 1 cut(s) 562
AgsI TTSAA 7 cut(s) 54, 66, 182, 209, 245, 1005, 1032
AjiI CACGTC 1 cut(s) 704
AjnI CCWGG 2 cut(s) 444, 829
Alw21I GWGCWC 3 cut(s) 423, 770, 897
Alw26I GTCTC 3 cut(s) 206, 640, 710
AlwI GGATC 2 cut(s) 665, 1031
AlwNI CAGNNNCTG 1 cut(s) 542
AoxI GGCC 3 cut(s) 680, 794, 964
ApaI GGGCCC 1 cut(s) 968
ApeKI GCWGC 8 cut(s) 39, 276, 385, 509, 512, 542, 653, 986
Asp700I GAANNNNTTC 1 cut(s) 845
AspLEI GCGC 1 cut(s) 903
AspS9I GGNCC 2 cut(s) 964, 965
AsuHPI GGTGA 3 cut(s) 127, 266, 329
BaeGI GKGCMC 2 cut(s) 412, 968
BanII GRGCYC 3 cut(s) 423, 897, 968
BbsI GAAGAC 1 cut(s) 924
Bbv12I GWGCWC 3 cut(s) 423, 770, 897
BbvCI CCTCAGC 1 cut(s) 195
BbvI GCAGC 8 cut(s) 51, 263, 372, 521, 524, 529, 665, 998
BccI CCATC 1 cut(s) 245
BceAI ACGGC 1 cut(s) 888
BciT130I CCWGG 2 cut(s) 446, 831
BciVI GTATCC 1 cut(s) 442
BcoDI GTCTC 3 cut(s) 206, 640, 710
BfaI CTAG 2 cut(s) 150, 395
BfmI CTRYAG 2 cut(s) 37, 386
BfrI CTTAAG 1 cut(s) 562
BfuI GTATCC 1 cut(s) 442
BisI GCNGC 8 cut(s) 40, 277, 386, 510, 513, 543, 654, 987
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 8 cut(s) 41, 278, 387, 511, 514, 544, 655, 988
Bme1390I CCNGG 2 cut(s) 446, 831
BmgBI CACGTC 1 cut(s) 704
BmgT120I GGNCC 2 cut(s) 964, 965
BmiI GGNNCC 2 cut(s) 698, 966
BmrFI CCNGG 2 cut(s) 446, 831
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 4 cut(s) 338, 490, 529, 901
BmuI ACTGGG 1 cut(s) 54
BpiI GAAGAC 1 cut(s) 924
BpmI CTGGAG 2 cut(s) 28, 525
Bpu10I CCTNAGC 1 cut(s) 195
Bpu1102I GCTNAGC 1 cut(s) 417
BpuEI CTTGAG 2 cut(s) 145, 944
BsaAI YACGTR 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 3 cut(s) 87, 677, 830
BsaWI WCCGGW 2 cut(s) 570, 806
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bsc4I CCNNNNNNNGG 5 cut(s) 144, 196, 313, 460, 503
Bse1I ACTGG 4 cut(s) 45, 60, 472, 657
Bse3DI GCAATG 1 cut(s) 127
BseBI CCWGG 2 cut(s) 446, 831
BseDI CCNNGG 3 cut(s) 87, 677, 830
BseGI GGATG 3 cut(s) 476, 724, 999
BseLI CCNNNNNNNGG 5 cut(s) 144, 196, 313, 460, 503
BseMI GCAATG 1 cut(s) 127
BseMII CTCAG 4 cut(s) 186, 344, 408, 755
BseNI ACTGG 4 cut(s) 45, 60, 472, 657
BseRI GAGGAG 1 cut(s) 212
BseSI GKGCMC 2 cut(s) 412, 968
BseXI GCAGC 8 cut(s) 51, 263, 372, 521, 524, 529, 665, 998
BseYI CCCAGC 1 cut(s) 381
BsgI GTGCAG 1 cut(s) 321
BshFI GGCC 3 cut(s) 682, 796, 966
BsiHKAI GWGCWC 3 cut(s) 423, 770, 897
BsiSI CCGG 2 cut(s) 571, 807
BslI CCNNNNNNNGG 5 cut(s) 144, 196, 313, 460, 503
BsmAI GTCTC 3 cut(s) 206, 640, 710
BsmBI CGTCTC 1 cut(s) 710
BsnI GGCC 3 cut(s) 682, 796, 966
Bso31I GGTCTC 1 cut(s) 206
Bsp120I GGGCCC 1 cut(s) 964
Bsp1286I GDGCHC 5 cut(s) 412, 423, 770, 897, 968
Bsp143I GATC 2 cut(s) 670, 1023
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 2 cut(s) 674, 754
BspANI GGCC 3 cut(s) 682, 796, 966
BspCNI CTCAG 4 cut(s) 187, 345, 409, 756
BspLI GGNNCC 2 cut(s) 698, 966
BspMAI CTGCAG 2 cut(s) 41, 390
BspPI GGATC 2 cut(s) 665, 1031
BspTI CTTAAG 1 cut(s) 562
BspTNI GGTCTC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 127
BsrI ACTGG 4 cut(s) 45, 60, 472, 657
BssECI CCNNGG 3 cut(s) 87, 677, 830
BssMI GATC 2 cut(s) 670, 1023
BssT1I CCWWGG 2 cut(s) 87, 677
Bst2UI CCWGG 2 cut(s) 446, 831
Bst4CI ACNGT 1 cut(s) 781
Bst6I CTCTTC 2 cut(s) 204, 1006
BstAFI CTTAAG 1 cut(s) 562
BstBAI YACGTR 1 cut(s) 12
BstC8I GCNNGC 3 cut(s) 83, 281, 559
BstDEI CTNAG 4 cut(s) 195, 353, 417, 764
BstENI CCTNNNNNAGG 1 cut(s) 458
BstF5I GGATG 3 cut(s) 476, 724, 999
BstHHI GCGC 1 cut(s) 903
BstKTI GATC 2 cut(s) 673, 1026
BstMAI GTCTC 3 cut(s) 206, 640, 710
BstMBI GATC 2 cut(s) 670, 1023
BstMWI GCNNNNNNNGC 1 cut(s) 509
BstNI CCWGG 2 cut(s) 446, 831
BstSCI CCNGG 2 cut(s) 444, 829
BstSFI CTRYAG 2 cut(s) 37, 386
BstSLI GKGCMC 2 cut(s) 412, 968
BstV1I GCAGC 8 cut(s) 51, 263, 372, 521, 524, 529, 665, 998
BstV2I GAAGAC 1 cut(s) 924
BstXI CCANNNNNNTGG 1 cut(s) 837
BsuI GTATCC 1 cut(s) 442
BsuRI GGCC 3 cut(s) 682, 796, 966
BtrI CACGTC 1 cut(s) 704
BtsCI GGATG 3 cut(s) 476, 724, 999
BtsIMutI CAGTG 1 cut(s) 718
Cac8I GCNNGC 3 cut(s) 83, 281, 559
CaiI CAGNNNCTG 1 cut(s) 542
CfoI GCGC 1 cut(s) 903
Cfr13I GGNCC 2 cut(s) 964, 965
Csp6I GTAC 3 cut(s) 13, 740, 757
CviAII CATG 3 cut(s) 220, 691, 837
CviQI GTAC 3 cut(s) 13, 740, 757
DdeI CTNAG 4 cut(s) 195, 353, 417, 764
DpnI GATC 2 cut(s) 672, 1025
DpnII GATC 2 cut(s) 670, 1023
Eam1104I CTCTTC 2 cut(s) 204, 1006
EarI CTCTTC 2 cut(s) 204, 1006
EciI GGCGGA 1 cut(s) 663
Ecl136II GAGCTC 2 cut(s) 421, 895
Eco130I CCWWGG 2 cut(s) 87, 677
Eco147I AGGCCT 2 cut(s) 682, 796
Eco24I GRGCYC 3 cut(s) 423, 897, 968
Eco31I GGTCTC 1 cut(s) 206
Eco32I GATATC 1 cut(s) 878
Eco53kI GAGCTC 2 cut(s) 421, 895
Eco57I CTGAAG 3 cut(s) 54, 327, 936
EcoICRI GAGCTC 2 cut(s) 421, 895
EcoNI CCTNNNNNAGG 1 cut(s) 458
EcoO109I RGGNCCY 1 cut(s) 964
EcoRII CCWGG 2 cut(s) 444, 829
EcoRV GATATC 1 cut(s) 878
EcoT14I CCWWGG 2 cut(s) 87, 677
EcoT38I GRGCYC 3 cut(s) 423, 897, 968
ErhI CCWWGG 2 cut(s) 87, 677
Esp3I CGTCTC 1 cut(s) 710
FaeI CATG 3 cut(s) 223, 694, 840
FalI AAGNNNNNCTT 2 cut(s) 833, 865
FatI CATG 3 cut(s) 219, 690, 836
FblI GTMKAC 1 cut(s) 783
Fnu4HI GCNGC 8 cut(s) 40, 277, 386, 510, 513, 543, 654, 987
FokI GGATG 3 cut(s) 483, 731, 1006
FriOI GRGCYC 3 cut(s) 423, 897, 968
Fsp4HI GCNGC 8 cut(s) 40, 277, 386, 510, 513, 543, 654, 987
FspBI CTAG 2 cut(s) 150, 395
GlaI GCGC 1 cut(s) 902
GluI GCNGC 8 cut(s) 40, 277, 386, 510, 513, 543, 654, 987
GsaI CCCAGC 1 cut(s) 385
GsuI CTGGAG 2 cut(s) 28, 525
HaeIII GGCC 3 cut(s) 682, 796, 966
HapII CCGG 2 cut(s) 571, 807
HhaI GCGC 1 cut(s) 903
Hin1II CATG 3 cut(s) 223, 694, 840
Hin6I GCGC 1 cut(s) 901
HinP1I GCGC 1 cut(s) 901
HindIII AAGCTT 1 cut(s) 141
HinfI GANTC 4 cut(s) 202, 552, 803, 953
HpaII CCGG 2 cut(s) 571, 807
HphI GGTGA 3 cut(s) 127, 266, 329
Hpy166II GTNNAC 2 cut(s) 534, 784
Hpy188I TCNGA 5 cut(s) 289, 627, 667, 765, 958
Hpy188III TCNNGA 7 cut(s) 162, 206, 345, 395, 497, 617, 908
Hpy8I GTNNAC 2 cut(s) 534, 784
HpyAV CCTTC 3 cut(s) 294, 398, 835
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4IV ACGT 2 cut(s) 11, 703
HpyCH4V TGCA 6 cut(s) 39, 120, 302, 329, 388, 653
HpyF10VI GCNNNNNNNGC 1 cut(s) 509
HpyF3I CTNAG 4 cut(s) 195, 353, 417, 764
HpySE526I ACGT 2 cut(s) 11, 703
Hsp92II CATG 3 cut(s) 223, 694, 840
HspAI GCGC 1 cut(s) 901
Kzo9I GATC 2 cut(s) 670, 1023
LmnI GCTCC 3 cut(s) 47, 506, 900
Lsp1109I GCAGC 8 cut(s) 51, 263, 372, 521, 524, 529, 665, 998
LweI GCATC 4 cut(s) 338, 490, 529, 901
MaeI CTAG 2 cut(s) 150, 395
MaeII ACGT 2 cut(s) 11, 703
MaeIII GTNAC 2 cut(s) 133, 566
MalI GATC 2 cut(s) 672, 1025
MboI GATC 2 cut(s) 670, 1023
MhlI GDGCHC 5 cut(s) 412, 423, 770, 897, 968
MluCI AATT 1 cut(s) 375
MmeI TCCRAC 3 cut(s) 612, 703, 709
MnlI CCTC 9 cut(s) 190, 289, 341, 454, 693, 709, 786, 943, 1014
MroXI GAANNNNTTC 1 cut(s) 845
MseI TTAA 6 cut(s) 111, 374, 483, 563, 735, 749
MspA1I CMGCKG 2 cut(s) 276, 385
MspCI CTTAAG 1 cut(s) 562
MspI CCGG 2 cut(s) 571, 807
MspR9I CCNGG 2 cut(s) 446, 831
MvaI CCWGG 2 cut(s) 446, 831
MwoI GCNNNNNNNGC 1 cut(s) 509
NdeII GATC 2 cut(s) 670, 1023
NlaIII CATG 3 cut(s) 223, 694, 840
NlaIV GGNNCC 2 cut(s) 698, 966
NmuCI GTSAC 2 cut(s) 133, 566
PceI AGGCCT 2 cut(s) 682, 796
PdmI GAANNNNTTC 1 cut(s) 845
PfeI GAWTC 4 cut(s) 202, 552, 803, 953
PflMI CCANNNNNTGG 1 cut(s) 144
PfoI TCCNGGA 1 cut(s) 444
PkrI GCNGC 8 cut(s) 41, 278, 387, 511, 514, 544, 655, 988
Ppu21I YACGTR 1 cut(s) 12
Psp124BI GAGCTC 2 cut(s) 423, 897
Psp6I CCWGG 2 cut(s) 444, 829
PspFI CCCAGC 1 cut(s) 381
PspGI CCWGG 2 cut(s) 444, 829
PspN4I GGNNCC 2 cut(s) 698, 966
PspOMI GGGCCC 1 cut(s) 964
PspPI GGNCC 2 cut(s) 964, 965
PstI CTGCAG 2 cut(s) 41, 390
PstNI CAGNNNCTG 1 cut(s) 542
PvuII CAGCTG 2 cut(s) 276, 385
RsaI GTAC 3 cut(s) 14, 741, 758
RsaNI GTAC 3 cut(s) 13, 740, 757
SacI GAGCTC 2 cut(s) 423, 897
SaqAI TTAA 6 cut(s) 111, 374, 483, 563, 735, 749
SatI GCNGC 8 cut(s) 40, 277, 386, 510, 513, 543, 654, 987
Sau3AI GATC 2 cut(s) 670, 1023
Sau96I GGNCC 2 cut(s) 964, 965
ScrFI CCNGG 2 cut(s) 446, 831
SduI GDGCHC 5 cut(s) 412, 423, 770, 897, 968
SfaNI GCATC 4 cut(s) 338, 490, 529, 901
SfcI CTRYAG 2 cut(s) 37, 386
SmlI CTYRAG 3 cut(s) 160, 562, 923
SmoI CTYRAG 3 cut(s) 160, 562, 923
Sse9I AATT 1 cut(s) 375
SseBI AGGCCT 2 cut(s) 682, 796
SsiI CCGC 2 cut(s) 674, 754
SspMI CTAG 2 cut(s) 150, 395
SstI GAGCTC 2 cut(s) 423, 897
StuI AGGCCT 2 cut(s) 682, 796
StyD4I CCNGG 2 cut(s) 444, 829
StyI CCWWGG 2 cut(s) 87, 677
TaaI ACNGT 1 cut(s) 781
TaiI ACGT 2 cut(s) 14, 706
TaqI TCGA 1 cut(s) 852
TasI AATT 1 cut(s) 375
TfiI GAWTC 4 cut(s) 202, 552, 803, 953
Tru1I TTAA 6 cut(s) 111, 374, 483, 563, 735, 749
Tru9I TTAA 6 cut(s) 111, 374, 483, 563, 735, 749
TscAI CASTG 1 cut(s) 718
TseFI GTSAC 2 cut(s) 133, 566
TseI GCWGC 8 cut(s) 39, 276, 385, 509, 512, 542, 653, 986
Tsp45I GTSAC 2 cut(s) 133, 566
TspDTI ATGAA 2 cut(s) 414, 1022
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 1 cut(s) 718
Van91I CCANNNNNTGG 1 cut(s) 144
Vha464I CTTAAG 1 cut(s) 562
XagI CCTNNNNNAGG 1 cut(s) 458
XbaI TCTAGA 1 cut(s) 394
XmiI GTMKAC 1 cut(s) 783
XmnI GAANNNNTTC 1 cut(s) 845
XspI CTAG 2 cut(s) 150, 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.