MD12G1219300.v1.1

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0009621
Physical Location & Seq
Reverse (-)
29653722 .. 29656431
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1219300.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGATGGCTTCCTCCACATCCCCAAACTGCTTGTTTCACTTCAGCTCCCTCTCTTCTTCTTCTTCAGTATCCAACTCAACCACCACCACACATTTGCCCTTATCTTCTTCTCACTCTATCTTCAATGGCTGCGGTGCACTTTCCCTCACCAAATCTTCTTCCTCTTCAAGAATATCTGCCAAGTTTGATAAGTTCCAAGGTGGAACTCCCCAGGAAATTCTTCAAGATGCCACACCACCACCATCTCTAGAAGCTCTTGAGGAAGAAGATGGTGAGCAGGAAGATGATGACAGCTGCTTGCCTTCTGACTTGGAGGGTGCAGTGCGTCAATCAGGTGTAGCAAGTGCAGATTTTGTGTCTTCAGGAGGAATGAGAGCCATAGTTGAGCTTTTGATCCCTCAGCTGCAATTTCTAGACGAAGAAGGTGCACAAGCTGAGCTCTGGGAATTGTCCAGGGTTTTCTTAGATACGCTGATCGAAGAAACCGGCGGTCAGAGAGTTAAAGCTGTGTTTCCTGATGCTGGTGCGGCAGCGCTTCTAAAATACCGTTGGAAAGATGCTGCTTTTGGATTCGCCAGCTTAAGTGACCGAAAGCCTATAACGAGTGAAGATGAGATTGTGGTTATGGTTGTTCCTGATTACCAGATGTTGGGATACGTCGAGAAACTTGCAGCTACTCTCTCAGATGACCCGCCAAGGCCTCTCATCATGTGGAACCCCCGTCTCATCAGCGAGGATGTAGGAGTTGGATTCAATGTTCGACAGTTAAGGCAGTACTTTCTCAGAACTTTTACGACGGTCTACTCAATGAGGCCAATGGAATCTGGTGCTGTATTTAGGTGTTATCCAGGGCTATGGAAGGTGTTCTACGATGACAAGGATAGACCGAACAGATACCTGCTCGCGAAAGAGCTCATTCAGCGCCCCGATGCTGAAGAACTTGAGATGATATTCGGAAATGTAGACGAGAGCTCAGAGAAGGGCCAATCTTTGTTTGATCAAGCTGCAGGGATGTTCTCTTCGCTAAATCGATTTATGAGGGTCATTTCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

352

Amino Acids

38.82

Weight (kDa)

4.64

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1995 PF09353 101 - 315 2.1e-52 Domain of unknown function (DUF1995)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 906
AccB7I CCANNNNNTGG 1 cut(s) 649
AccI GTMKAC 2 cut(s) 801, 963
AccII CGCG 1 cut(s) 905
AciI CCGC 4 cut(s) 132, 489, 527, 692
AclWI GGATC 1 cut(s) 388
AcsI RAATTY 1 cut(s) 216
AcuI CTGAAG 4 cut(s) 25, 48, 345, 954
AfaI GTAC 1 cut(s) 776
AfeI AGCGCT 1 cut(s) 534
AfiI CCNNNNNNNGG 2 cut(s) 521, 649
AflII CTTAAG 1 cut(s) 580
AgsI TTSAA 5 cut(s) 124, 168, 224, 754, 1050
AjnI CCWGG 3 cut(s) 210, 452, 847
AloI GAACNNNNNNTCC 2 cut(s) 741, 773
Alw21I GWGCWC 5 cut(s) 139, 430, 441, 915, 974
Alw26I GTCTC 1 cut(s) 728
Alw44I GTGCAC 2 cut(s) 135, 426
AlwI GGATC 1 cut(s) 388
Aor51HI AGCGCT 1 cut(s) 534
AoxI GGCC 3 cut(s) 698, 812, 982
ApaLI GTGCAC 2 cut(s) 135, 426
ApeKI GCWGC 7 cut(s) 129, 294, 403, 530, 560, 671, 1004
ApoI RAATTY 1 cut(s) 216
Asp700I GAANNNNTTC 2 cut(s) 219, 863
AspLEI GCGC 2 cut(s) 535, 924
AspS9I GGNCC 1 cut(s) 982
AsuHPI GGTGA 2 cut(s) 139, 284
BaeGI GKGCMC 2 cut(s) 139, 430
BanII GRGCYC 3 cut(s) 441, 915, 974
BarI GAAGNNNNNNTAC 2 cut(s) 851, 883
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 5 cut(s) 139, 430, 441, 915, 974
BbvCI CCTCAGC 1 cut(s) 399
BbvI GCAGC 7 cut(s) 116, 281, 390, 542, 547, 683, 991
BccI CCATC 2 cut(s) 250, 263
BcgI CGANNNNNNTGC 4 cut(s) 407, 441, 650, 684
BciT130I CCWGG 3 cut(s) 212, 454, 849
BciVI GTATCC 2 cut(s) 79, 647
BclI TGATCA 1 cut(s) 997
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 2 cut(s) 248, 413
BfmI CTRYAG 1 cut(s) 1005
BfoI RGCGCY 2 cut(s) 536, 925
BfrI CTTAAG 1 cut(s) 580
BfuAI ACCTGC 1 cut(s) 906
BfuI GTATCC 2 cut(s) 79, 647
BisI GCNGC 8 cut(s) 130, 295, 404, 528, 531, 561, 672, 1005
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 8 cut(s) 131, 296, 405, 529, 532, 562, 673, 1006
BmcAI AGTACT 1 cut(s) 776
Bme1390I CCNGG 3 cut(s) 212, 454, 849
BmgT120I GGNCC 1 cut(s) 982
BmiI GGNNCC 1 cut(s) 716
BmrFI CCNGG 3 cut(s) 212, 454, 849
BmsI GCATC 4 cut(s) 217, 508, 547, 919
BpiI GAAGAC 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 399
Bpu1102I GCTNAGC 1 cut(s) 435
BpuEI CTTGAG 2 cut(s) 278, 962
Bsa29I ATCGAT 1 cut(s) 1030
BsaJI CCNNGG 5 cut(s) 196, 210, 453, 695, 848
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 2 cut(s) 521, 649
Bse118I RCCGGY 1 cut(s) 485
BseBI CCWGG 3 cut(s) 212, 454, 849
BseCI ATCGAT 1 cut(s) 1030
BseDI CCNNGG 5 cut(s) 196, 210, 453, 695, 848
BseGI GGATG 3 cut(s) 17, 742, 1017
BseLI CCNNNNNNNGG 2 cut(s) 521, 649
BseMII CTCAG 5 cut(s) 413, 426, 696, 796, 987
BseSI GKGCMC 2 cut(s) 139, 430
BseXI GCAGC 7 cut(s) 116, 281, 390, 542, 547, 683, 991
BsgI GTGCAG 2 cut(s) 339, 366
Bsh1236I CGCG 1 cut(s) 905
BshFI GGCC 3 cut(s) 700, 814, 984
BshVI ATCGAT 1 cut(s) 1030
BsiHKAI GWGCWC 5 cut(s) 139, 430, 441, 915, 974
BsiSI CCGG 1 cut(s) 486
BslI CCNNNNNNNGG 2 cut(s) 521, 649
BsmAI GTCTC 1 cut(s) 728
BsmBI CGTCTC 1 cut(s) 728
BsnI GGCC 3 cut(s) 700, 814, 984
Bsp1286I GDGCHC 5 cut(s) 139, 430, 441, 915, 974
Bsp143I GATC 3 cut(s) 393, 474, 997
Bsp1720I GCTNAGC 1 cut(s) 435
Bsp68I TCGCGA 1 cut(s) 905
BspACI CCGC 4 cut(s) 132, 489, 527, 692
BspANI GGCC 3 cut(s) 700, 814, 984
BspCNI CTCAG 5 cut(s) 412, 427, 695, 795, 986
BspDI ATCGAT 1 cut(s) 1030
BspFNI CGCG 1 cut(s) 905
BspLI GGNNCC 1 cut(s) 716
BspMAI CTGCAG 1 cut(s) 1009
BspMI ACCTGC 1 cut(s) 906
BspPI GGATC 1 cut(s) 388
BspTI CTTAAG 1 cut(s) 580
BsrFI RCCGGY 1 cut(s) 485
BssAI RCCGGY 1 cut(s) 485
BssECI CCNNGG 5 cut(s) 196, 210, 453, 695, 848
BssMI GATC 3 cut(s) 393, 474, 997
BssT1I CCWWGG 2 cut(s) 196, 695
Bst2UI CCWGG 3 cut(s) 212, 454, 849
Bst4CI ACNGT 3 cut(s) 548, 765, 799
Bst6I CTCTTC 3 cut(s) 58, 169, 1024
BstAFI CTTAAG 1 cut(s) 580
BstC8I GCNNGC 3 cut(s) 299, 577, 903
BstDEI CTNAG 6 cut(s) 399, 435, 463, 682, 782, 973
BstF5I GGATG 3 cut(s) 17, 742, 1017
BstFNI CGCG 1 cut(s) 905
BstH2I RGCGCY 2 cut(s) 536, 925
BstHHI GCGC 2 cut(s) 535, 924
BstKTI GATC 3 cut(s) 396, 477, 1000
BstMAI GTCTC 1 cut(s) 728
BstMBI GATC 3 cut(s) 393, 474, 997
BstMWI GCNNNNNNNGC 2 cut(s) 527, 919
BstNI CCWGG 3 cut(s) 212, 454, 849
BstSCI CCNGG 3 cut(s) 210, 452, 847
BstSFI CTRYAG 1 cut(s) 1005
BstSLI GKGCMC 2 cut(s) 139, 430
BstUI CGCG 1 cut(s) 905
BstV1I GCAGC 7 cut(s) 116, 281, 390, 542, 547, 683, 991
BstV2I GAAGAC 1 cut(s) 351
BstXI CCANNNNNNTGG 1 cut(s) 855
Bsu15I ATCGAT 1 cut(s) 1030
BsuI GTATCC 2 cut(s) 79, 647
BsuRI GGCC 3 cut(s) 700, 814, 984
BsuTUI ATCGAT 1 cut(s) 1030
BtsCI GGATG 3 cut(s) 17, 742, 1017
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 1 cut(s) 327
BtuMI TCGCGA 1 cut(s) 905
BveI ACCTGC 1 cut(s) 906
Cac8I GCNNGC 3 cut(s) 299, 577, 903
CfoI GCGC 2 cut(s) 535, 924
Cfr10I RCCGGY 1 cut(s) 485
Cfr13I GGNCC 1 cut(s) 982
ClaI ATCGAT 1 cut(s) 1030
CseI GACGC 1 cut(s) 314
Csp6I GTAC 1 cut(s) 775
CviAII CATG 1 cut(s) 709
CviQI GTAC 1 cut(s) 775
DdeI CTNAG 6 cut(s) 399, 435, 463, 682, 782, 973
DpnI GATC 3 cut(s) 395, 476, 999
DpnII GATC 3 cut(s) 393, 474, 997
Eam1104I CTCTTC 3 cut(s) 58, 169, 1024
EarI CTCTTC 3 cut(s) 58, 169, 1024
Ecl136II GAGCTC 3 cut(s) 439, 913, 972
Eco130I CCWWGG 2 cut(s) 196, 695
Eco147I AGGCCT 1 cut(s) 700
Eco24I GRGCYC 3 cut(s) 441, 915, 974
Eco47III AGCGCT 1 cut(s) 534
Eco53kI GAGCTC 3 cut(s) 439, 913, 972
Eco57I CTGAAG 4 cut(s) 25, 48, 345, 954
EcoICRI GAGCTC 3 cut(s) 439, 913, 972
EcoRII CCWGG 3 cut(s) 210, 452, 847
EcoT14I CCWWGG 2 cut(s) 196, 695
EcoT38I GRGCYC 3 cut(s) 441, 915, 974
ErhI CCWWGG 2 cut(s) 196, 695
Esp3I CGTCTC 1 cut(s) 728
FaeI CATG 1 cut(s) 712
FaiI YATR 6 cut(s) 380, 599, 626, 710, 856, 1037
FatI CATG 1 cut(s) 708
FauI CCCGC 1 cut(s) 699
FbaI TGATCA 1 cut(s) 997
FblI GTMKAC 2 cut(s) 801, 963
Fnu4HI GCNGC 8 cut(s) 130, 295, 404, 528, 531, 561, 672, 1005
FokI GGATG 3 cut(s) 4, 749, 1024
FriOI GRGCYC 3 cut(s) 441, 915, 974
Fsp4HI GCNGC 8 cut(s) 130, 295, 404, 528, 531, 561, 672, 1005
FspBI CTAG 2 cut(s) 248, 413
GlaI GCGC 2 cut(s) 534, 923
GluI GCNGC 8 cut(s) 130, 295, 404, 528, 531, 561, 672, 1005
HaeII RGCGCY 2 cut(s) 536, 925
HaeIII GGCC 3 cut(s) 700, 814, 984
HapII CCGG 1 cut(s) 486
HgaI GACGC 1 cut(s) 314
HhaI GCGC 2 cut(s) 535, 924
Hin1II CATG 1 cut(s) 712
Hin6I GCGC 2 cut(s) 533, 922
HinP1I GCGC 2 cut(s) 533, 922
HinfI GANTC 3 cut(s) 570, 750, 821
HpaII CCGG 1 cut(s) 486
HphI GGTGA 2 cut(s) 139, 284
Hpy166II GTNNAC 4 cut(s) 137, 428, 802, 964
Hpy188I TCNGA 6 cut(s) 307, 495, 685, 785, 956, 976
Hpy8I GTNNAC 4 cut(s) 137, 428, 802, 964
Hpy99I CGWCG 2 cut(s) 662, 799
HpyAV CCTTC 4 cut(s) 312, 416, 853, 973
HpyCH4III ACNGT 3 cut(s) 548, 765, 799
HpyCH4IV ACGT 1 cut(s) 657
HpyCH4V TGCA 7 cut(s) 137, 320, 347, 406, 428, 671, 1007
HpyF10VI GCNNNNNNNGC 2 cut(s) 527, 919
HpyF3I CTNAG 6 cut(s) 399, 435, 463, 682, 782, 973
HpySE526I ACGT 1 cut(s) 657
Hsp92II CATG 1 cut(s) 712
HspAI GCGC 2 cut(s) 533, 922
Ksp22I TGATCA 1 cut(s) 997
Kzo9I GATC 3 cut(s) 393, 474, 997
LmnI GCTCC 1 cut(s) 50
Lsp1109I GCAGC 7 cut(s) 116, 281, 390, 542, 547, 683, 991
LweI GCATC 4 cut(s) 217, 508, 547, 919
MaeI CTAG 2 cut(s) 248, 413
MaeII ACGT 1 cut(s) 657
MaeIII GTNAC 1 cut(s) 584
MalI GATC 3 cut(s) 395, 476, 999
MboI GATC 3 cut(s) 393, 474, 997
MhlI GDGCHC 5 cut(s) 139, 430, 441, 915, 974
MluCI AATT 3 cut(s) 216, 407, 446
MmeI TCCRAC 3 cut(s) 96, 530, 727
MroXI GAANNNNTTC 2 cut(s) 219, 863
MseI TTAA 3 cut(s) 501, 581, 767
MspA1I CMGCKG 2 cut(s) 294, 403
MspCI CTTAAG 1 cut(s) 580
MspI CCGG 1 cut(s) 486
MspR9I CCNGG 3 cut(s) 212, 454, 849
MvaI CCWGG 3 cut(s) 212, 454, 849
MvnI CGCG 1 cut(s) 905
MwoI GCNNNNNNNGC 2 cut(s) 527, 919
NdeII GATC 3 cut(s) 393, 474, 997
NlaIII CATG 1 cut(s) 712
NlaIV GGNNCC 1 cut(s) 716
NmuCI GTSAC 1 cut(s) 584
NruI TCGCGA 1 cut(s) 905
PceI AGGCCT 1 cut(s) 700
PdmI GAANNNNTTC 2 cut(s) 219, 863
PfeI GAWTC 3 cut(s) 570, 750, 821
PflMI CCANNNNNTGG 1 cut(s) 649
PkrI GCNGC 8 cut(s) 131, 296, 405, 529, 532, 562, 673, 1006
Psp124BI GAGCTC 3 cut(s) 441, 915, 974
Psp6I CCWGG 3 cut(s) 210, 452, 847
PspGI CCWGG 3 cut(s) 210, 452, 847
PspN4I GGNNCC 1 cut(s) 716
PspPI GGNCC 1 cut(s) 982
PstI CTGCAG 1 cut(s) 1009
PvuII CAGCTG 2 cut(s) 294, 403
RruI TCGCGA 1 cut(s) 905
RsaI GTAC 1 cut(s) 776
RsaNI GTAC 1 cut(s) 775
SacI GAGCTC 3 cut(s) 441, 915, 974
SaqAI TTAA 3 cut(s) 501, 581, 767
SatI GCNGC 8 cut(s) 130, 295, 404, 528, 531, 561, 672, 1005
Sau3AI GATC 3 cut(s) 393, 474, 997
Sau96I GGNCC 1 cut(s) 982
ScaI AGTACT 1 cut(s) 776
ScrFI CCNGG 3 cut(s) 212, 454, 849
SduI GDGCHC 5 cut(s) 139, 430, 441, 915, 974
SfaNI GCATC 4 cut(s) 217, 508, 547, 919
SfcI CTRYAG 1 cut(s) 1005
SmlI CTYRAG 3 cut(s) 257, 580, 941
SmoI CTYRAG 3 cut(s) 257, 580, 941
Sse9I AATT 3 cut(s) 216, 407, 446
SseBI AGGCCT 1 cut(s) 700
SsiI CCGC 4 cut(s) 132, 489, 527, 692
SspMI CTAG 2 cut(s) 248, 413
SstI GAGCTC 3 cut(s) 441, 915, 974
StuI AGGCCT 1 cut(s) 700
StyD4I CCNGG 3 cut(s) 210, 452, 847
StyI CCWWGG 2 cut(s) 196, 695
TaaI ACNGT 3 cut(s) 548, 765, 799
TaiI ACGT 1 cut(s) 660
TaqI TCGA 4 cut(s) 477, 660, 760, 1030
TaqII GACCGA 2 cut(s) 603, 901
TasI AATT 3 cut(s) 216, 407, 446
TatI WGTACW 1 cut(s) 774
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 3 cut(s) 570, 750, 821
Tru1I TTAA 3 cut(s) 501, 581, 767
Tru9I TTAA 3 cut(s) 501, 581, 767
TscAI CASTG 1 cut(s) 327
TseFI GTSAC 1 cut(s) 584
TseI GCWGC 7 cut(s) 129, 294, 403, 530, 560, 671, 1004
Tsp45I GTSAC 1 cut(s) 584
TspRI CASTG 1 cut(s) 327
Van91I CCANNNNNTGG 1 cut(s) 649
Vha464I CTTAAG 1 cut(s) 580
VneI GTGCAC 2 cut(s) 135, 426
XapI RAATTY 1 cut(s) 216
XbaI TCTAGA 2 cut(s) 247, 412
XmiI GTMKAC 2 cut(s) 801, 963
XmnI GAANNNNTTC 2 cut(s) 219, 863
XspI CTAG 2 cut(s) 248, 413
ZrmI AGTACT 1 cut(s) 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.