RLG00000025418

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0009621
Physical Location & Seq
Reverse (-)
45665516 .. 45668882
3367 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025418

Sequence Viewer

Length: 1041 bp
ATGGCTTCCACGTACCCAAGTTCCCTGTTTTACTTCTGCAGCTCCAGTTCTTCAAAACCCAGTTCAACTTCAGCCACACGCTTGCCCTTGACTTTTTCTGGGTCTGGCTTTAATGGGTGCAATGGAGTTTCAGTCACCAAAGCTTGGTCTAGTAGTAGCTCAAGAATATATGCCAAGTTTGAAAAGTTCCAAGCTGAGGAGAATCTTGAAGAGACCAGCATGCCATCATCATCTTTCCAAGAAGTTGAAGAAGATGGTGAGCAGGAAGAAGATGACAGCTGCTTGCCTTCGGACTTGGAGGGTGCAGTCCGTCAATCGGGTGAAGCAAGTGCATCATTTGTTTCTTCAGGAGGACTGAGAGCCATAGTGGAGCTTTTAATTCCCCAGCTGCAGTTTCTAGATGAAGAAGGGGCACAAGCTGAGCTCTGGGAGTTGTCAAGGGTTTTCCTGGATACCCTTATAGAGGAAACTGGATGTGAGAGAGTTAAAGCTGTATTTCCTGATGCTGGAGCGGCAGCACTACTAAAATATAAGTGGACAGATGCTGCCTTTGGATTTGCCAGCTTAAGTGACCGGAAACCTATAACAAGTGAAGATGAGATTGTGGTTATGATTGTTCCTGATTATCAGATGTTGGAATATGTAGAGAGACTTGCAGCCAGTCTTTCAGATGATCCGCCAAGGCCTCTAATCATGTGGAACCCACGTCTCATCAGTGAGGATGTTGGAGTTGGATTTAATGTACGGAAGTTAAGGCGGTACTTTCTGAGCACTTTTACGACGGTCTACTCAATGAGGCCTATGGAATCCGGTGCTGTATTTAGGTGCTATCCAGGATCATGGAAGGTATTCTTCGATGATAAGGATAGACCAAACAGATATCTACTTGCCAAAGAGCTCCTGCGCCGTCCTGATGCTGAAGACCTTGAGATGATATTTGGAAATATGCAAGAGGATTCAGAGCAGGGCCCATCTTTGTTTGATAAAGCAGCAGGGATGTTCTCTTCAATGAACCGATTTATGAGGATCATTTCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

347

Amino Acids

38.68

Weight (kDa)

4.6

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1995 PF09353 96 - 310 2.5e-51 Domain of unknown function (DUF1995)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 144
AccBSI CCGCTC 1 cut(s) 512
AccI GTMKAC 1 cut(s) 786
AciI CCGC 3 cut(s) 512, 677, 757
AclWI GGATC 3 cut(s) 668, 844, 1034
AcuI CTGAAG 3 cut(s) 54, 330, 939
AfaI GTAC 3 cut(s) 14, 744, 761
AfiI CCNNNNNNNGG 5 cut(s) 144, 196, 316, 463, 506
AflII CTTAAG 1 cut(s) 565
AgsI TTSAA 7 cut(s) 54, 66, 182, 209, 248, 1008, 1035
AjiI CACGTC 1 cut(s) 707
AjnI CCWGG 2 cut(s) 447, 832
Alw21I GWGCWC 3 cut(s) 426, 773, 900
Alw26I GTCTC 3 cut(s) 206, 643, 713
AlwI GGATC 3 cut(s) 668, 844, 1034
AlwNI CAGNNNCTG 1 cut(s) 545
AoxI GGCC 3 cut(s) 683, 797, 967
ApaI GGGCCC 1 cut(s) 971
ApeKI GCWGC 7 cut(s) 39, 279, 388, 515, 545, 656, 989
Asp700I GAANNNNTTC 1 cut(s) 848
AspLEI GCGC 1 cut(s) 906
AspS9I GGNCC 2 cut(s) 967, 968
AsuHPI GGTGA 3 cut(s) 127, 269, 332
BaeGI GKGCMC 2 cut(s) 415, 971
BanII GRGCYC 3 cut(s) 426, 900, 971
BbsI GAAGAC 1 cut(s) 927
Bbv12I GWGCWC 3 cut(s) 426, 773, 900
BbvCI CCTCAGC 1 cut(s) 195
BbvI GCAGC 7 cut(s) 51, 266, 375, 527, 532, 668, 1001
BccI CCATC 3 cut(s) 232, 248, 979
BceAI ACGGC 1 cut(s) 891
BciT130I CCWGG 2 cut(s) 449, 834
BciVI GTATCC 1 cut(s) 445
BcoDI GTCTC 3 cut(s) 206, 643, 713
BfaI CTAG 2 cut(s) 150, 398
BfmI CTRYAG 2 cut(s) 37, 389
BfrI CTTAAG 1 cut(s) 565
BfuI GTATCC 1 cut(s) 445
BisI GCNGC 8 cut(s) 40, 280, 389, 513, 516, 546, 657, 990
BlpI GCTNAGC 1 cut(s) 420
BlsI GCNGC 8 cut(s) 41, 281, 390, 514, 517, 547, 658, 991
Bme1390I CCNGG 2 cut(s) 449, 834
BmgBI CACGTC 1 cut(s) 707
BmgT120I GGNCC 2 cut(s) 967, 968
BmiI GGNNCC 2 cut(s) 701, 969
BmrFI CCNGG 2 cut(s) 449, 834
BmrI ACTGGG 1 cut(s) 54
BmsI GCATC 4 cut(s) 341, 493, 532, 904
BmuI ACTGGG 1 cut(s) 54
BpiI GAAGAC 1 cut(s) 927
BpmI CTGGAG 2 cut(s) 28, 528
Bpu10I CCTNAGC 1 cut(s) 195
Bpu1102I GCTNAGC 1 cut(s) 420
BpuEI CTTGAG 2 cut(s) 145, 947
BsaAI YACGTR 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 1 cut(s) 680
BsaWI WCCGGW 2 cut(s) 573, 809
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bsc4I CCNNNNNNNGG 5 cut(s) 144, 196, 316, 463, 506
Bse1I ACTGG 4 cut(s) 45, 60, 475, 660
Bse3DI GCAATG 1 cut(s) 127
BseBI CCWGG 2 cut(s) 449, 834
BseDI CCNNGG 1 cut(s) 680
BseGI GGATG 3 cut(s) 479, 727, 1002
BseLI CCNNNNNNNGG 5 cut(s) 144, 196, 316, 463, 506
BseMI GCAATG 1 cut(s) 127
BseMII CTCAG 4 cut(s) 186, 347, 411, 758
BseNI ACTGG 4 cut(s) 45, 60, 475, 660
BseRI GAGGAG 1 cut(s) 212
BseSI GKGCMC 2 cut(s) 415, 971
BseXI GCAGC 7 cut(s) 51, 266, 375, 527, 532, 668, 1001
BseYI CCCAGC 1 cut(s) 384
BsgI GTGCAG 1 cut(s) 324
BshFI GGCC 3 cut(s) 685, 799, 969
BsiHKAI GWGCWC 3 cut(s) 426, 773, 900
BsiSI CCGG 2 cut(s) 574, 810
BslI CCNNNNNNNGG 5 cut(s) 144, 196, 316, 463, 506
BsmAI GTCTC 3 cut(s) 206, 643, 713
BsmBI CGTCTC 1 cut(s) 713
BsnI GGCC 3 cut(s) 685, 799, 969
Bso31I GGTCTC 1 cut(s) 206
Bsp120I GGGCCC 1 cut(s) 967
Bsp1286I GDGCHC 5 cut(s) 415, 426, 773, 900, 971
Bsp143I GATC 3 cut(s) 673, 836, 1026
Bsp1720I GCTNAGC 1 cut(s) 420
BspACI CCGC 3 cut(s) 512, 677, 757
BspANI GGCC 3 cut(s) 685, 799, 969
BspCNI CTCAG 4 cut(s) 187, 348, 412, 759
BspLI GGNNCC 2 cut(s) 701, 969
BspMAI CTGCAG 2 cut(s) 41, 393
BspPI GGATC 3 cut(s) 668, 844, 1034
BspTI CTTAAG 1 cut(s) 565
BspTNI GGTCTC 1 cut(s) 206
BsrBI CCGCTC 1 cut(s) 512
BsrDI GCAATG 1 cut(s) 127
BsrI ACTGG 4 cut(s) 45, 60, 475, 660
BssECI CCNNGG 1 cut(s) 680
BssMI GATC 3 cut(s) 673, 836, 1026
BssT1I CCWWGG 1 cut(s) 680
Bst2UI CCWGG 2 cut(s) 449, 834
Bst4CI ACNGT 1 cut(s) 784
Bst6I CTCTTC 2 cut(s) 204, 1009
BstAFI CTTAAG 1 cut(s) 565
BstBAI YACGTR 1 cut(s) 12
BstC8I GCNNGC 4 cut(s) 83, 221, 284, 562
BstDEI CTNAG 4 cut(s) 195, 356, 420, 767
BstENI CCTNNNNNAGG 1 cut(s) 461
BstF5I GGATG 3 cut(s) 479, 727, 1002
BstHHI GCGC 1 cut(s) 906
BstKTI GATC 3 cut(s) 676, 839, 1029
BstMAI GTCTC 3 cut(s) 206, 643, 713
BstMBI GATC 3 cut(s) 673, 836, 1026
BstMWI GCNNNNNNNGC 1 cut(s) 512
BstNI CCWGG 2 cut(s) 449, 834
BstNSI RCATGY 1 cut(s) 223
BstSCI CCNGG 2 cut(s) 447, 832
BstSFI CTRYAG 2 cut(s) 37, 389
BstSLI GKGCMC 2 cut(s) 415, 971
BstV1I GCAGC 7 cut(s) 51, 266, 375, 527, 532, 668, 1001
BstV2I GAAGAC 1 cut(s) 927
BstXI CCANNNNNNTGG 1 cut(s) 840
BsuI GTATCC 1 cut(s) 445
BsuRI GGCC 3 cut(s) 685, 799, 969
BtrI CACGTC 1 cut(s) 707
BtsCI GGATG 3 cut(s) 479, 727, 1002
BtsIMutI CAGTG 1 cut(s) 721
Cac8I GCNNGC 4 cut(s) 83, 221, 284, 562
CaiI CAGNNNCTG 1 cut(s) 545
CfoI GCGC 1 cut(s) 906
Cfr13I GGNCC 2 cut(s) 967, 968
Csp6I GTAC 3 cut(s) 13, 743, 760
CviAII CATG 3 cut(s) 220, 694, 840
CviQI GTAC 3 cut(s) 13, 743, 760
DdeI CTNAG 4 cut(s) 195, 356, 420, 767
DpnI GATC 3 cut(s) 675, 838, 1028
DpnII GATC 3 cut(s) 673, 836, 1026
Eam1104I CTCTTC 2 cut(s) 204, 1009
EarI CTCTTC 2 cut(s) 204, 1009
EciI GGCGGA 1 cut(s) 666
Ecl136II GAGCTC 2 cut(s) 424, 898
Eco130I CCWWGG 1 cut(s) 680
Eco147I AGGCCT 2 cut(s) 685, 799
Eco24I GRGCYC 3 cut(s) 426, 900, 971
Eco31I GGTCTC 1 cut(s) 206
Eco32I GATATC 1 cut(s) 881
Eco53kI GAGCTC 2 cut(s) 424, 898
Eco57I CTGAAG 3 cut(s) 54, 330, 939
EcoICRI GAGCTC 2 cut(s) 424, 898
EcoNI CCTNNNNNAGG 1 cut(s) 461
EcoO109I RGGNCCY 1 cut(s) 967
EcoRII CCWGG 2 cut(s) 447, 832
EcoRV GATATC 1 cut(s) 881
EcoT14I CCWWGG 1 cut(s) 680
EcoT38I GRGCYC 3 cut(s) 426, 900, 971
ErhI CCWWGG 1 cut(s) 680
Esp3I CGTCTC 1 cut(s) 713
FaeI CATG 3 cut(s) 223, 697, 843
FalI AAGNNNNNCTT 2 cut(s) 836, 868
FatI CATG 3 cut(s) 219, 693, 839
FblI GTMKAC 1 cut(s) 786
Fnu4HI GCNGC 8 cut(s) 40, 280, 389, 513, 516, 546, 657, 990
FokI GGATG 3 cut(s) 486, 734, 1009
FriOI GRGCYC 3 cut(s) 426, 900, 971
Fsp4HI GCNGC 8 cut(s) 40, 280, 389, 513, 516, 546, 657, 990
FspBI CTAG 2 cut(s) 150, 398
GlaI GCGC 1 cut(s) 905
GluI GCNGC 8 cut(s) 40, 280, 389, 513, 516, 546, 657, 990
GsaI CCCAGC 1 cut(s) 388
GsuI CTGGAG 2 cut(s) 28, 528
HaeIII GGCC 3 cut(s) 685, 799, 969
HapII CCGG 2 cut(s) 574, 810
HhaI GCGC 1 cut(s) 906
Hin1II CATG 3 cut(s) 223, 697, 843
Hin6I GCGC 1 cut(s) 904
HinP1I GCGC 1 cut(s) 904
HindIII AAGCTT 1 cut(s) 141
HinfI GANTC 3 cut(s) 202, 806, 956
HpaII CCGG 2 cut(s) 574, 810
HphI GGTGA 3 cut(s) 127, 269, 332
Hpy166II GTNNAC 2 cut(s) 537, 787
Hpy188I TCNGA 5 cut(s) 292, 630, 670, 768, 961
Hpy188III TCNNGA 7 cut(s) 162, 206, 348, 398, 500, 620, 911
Hpy8I GTNNAC 2 cut(s) 537, 787
Hpy99I CGWCG 1 cut(s) 784
HpyAV CCTTC 3 cut(s) 297, 401, 838
HpyCH4III ACNGT 1 cut(s) 784
HpyCH4IV ACGT 2 cut(s) 11, 706
HpyCH4V TGCA 7 cut(s) 39, 120, 305, 332, 391, 656, 949
HpyF10VI GCNNNNNNNGC 1 cut(s) 512
HpyF3I CTNAG 4 cut(s) 195, 356, 420, 767
HpySE526I ACGT 2 cut(s) 11, 706
Hsp92II CATG 3 cut(s) 223, 697, 843
HspAI GCGC 1 cut(s) 904
Kzo9I GATC 3 cut(s) 673, 836, 1026
LmnI GCTCC 4 cut(s) 47, 370, 509, 903
Lsp1109I GCAGC 7 cut(s) 51, 266, 375, 527, 532, 668, 1001
LweI GCATC 4 cut(s) 341, 493, 532, 904
MaeI CTAG 2 cut(s) 150, 398
MaeII ACGT 2 cut(s) 11, 706
MaeIII GTNAC 2 cut(s) 133, 569
MalI GATC 3 cut(s) 675, 838, 1028
MbiI CCGCTC 1 cut(s) 512
MboI GATC 3 cut(s) 673, 836, 1026
MhlI GDGCHC 5 cut(s) 415, 426, 773, 900, 971
MluCI AATT 1 cut(s) 378
MmeI TCCRAC 3 cut(s) 615, 706, 712
MnlI CCTC 9 cut(s) 190, 292, 344, 457, 696, 712, 789, 946, 1017
MroXI GAANNNNTTC 1 cut(s) 848
MseI TTAA 6 cut(s) 111, 377, 486, 566, 738, 752
MspA1I CMGCKG 2 cut(s) 279, 388
MspCI CTTAAG 1 cut(s) 565
MspI CCGG 2 cut(s) 574, 810
MspR9I CCNGG 2 cut(s) 449, 834
MvaI CCWGG 2 cut(s) 449, 834
MwoI GCNNNNNNNGC 1 cut(s) 512
NdeII GATC 3 cut(s) 673, 836, 1026
NlaIII CATG 3 cut(s) 223, 697, 843
NlaIV GGNNCC 2 cut(s) 701, 969
NmuCI GTSAC 2 cut(s) 133, 569
NspI RCATGY 1 cut(s) 223
PaeI GCATGC 1 cut(s) 223
PceI AGGCCT 2 cut(s) 685, 799
PdmI GAANNNNTTC 1 cut(s) 848
PfeI GAWTC 3 cut(s) 202, 806, 956
PflMI CCANNNNNTGG 1 cut(s) 144
PfoI TCCNGGA 2 cut(s) 447, 832
PkrI GCNGC 8 cut(s) 41, 281, 390, 514, 517, 547, 658, 991
Ppu21I YACGTR 1 cut(s) 12
Psp124BI GAGCTC 2 cut(s) 426, 900
Psp6I CCWGG 2 cut(s) 447, 832
PspFI CCCAGC 1 cut(s) 384
PspGI CCWGG 2 cut(s) 447, 832
PspN4I GGNNCC 2 cut(s) 701, 969
PspOMI GGGCCC 1 cut(s) 967
PspPI GGNCC 2 cut(s) 967, 968
PstI CTGCAG 2 cut(s) 41, 393
PstNI CAGNNNCTG 1 cut(s) 545
PvuII CAGCTG 2 cut(s) 279, 388
RsaI GTAC 3 cut(s) 14, 744, 761
RsaNI GTAC 3 cut(s) 13, 743, 760
SacI GAGCTC 2 cut(s) 426, 900
SaqAI TTAA 6 cut(s) 111, 377, 486, 566, 738, 752
SatI GCNGC 8 cut(s) 40, 280, 389, 513, 516, 546, 657, 990
Sau3AI GATC 3 cut(s) 673, 836, 1026
Sau96I GGNCC 2 cut(s) 967, 968
ScrFI CCNGG 2 cut(s) 449, 834
SduI GDGCHC 5 cut(s) 415, 426, 773, 900, 971
SfaNI GCATC 4 cut(s) 341, 493, 532, 904
SfcI CTRYAG 2 cut(s) 37, 389
SmlI CTYRAG 3 cut(s) 160, 565, 926
SmoI CTYRAG 3 cut(s) 160, 565, 926
SphI GCATGC 1 cut(s) 223
Sse9I AATT 1 cut(s) 378
SseBI AGGCCT 2 cut(s) 685, 799
SsiI CCGC 3 cut(s) 512, 677, 757
SspMI CTAG 2 cut(s) 150, 398
SstI GAGCTC 2 cut(s) 426, 900
StuI AGGCCT 2 cut(s) 685, 799
StyD4I CCNGG 2 cut(s) 447, 832
StyI CCWWGG 1 cut(s) 680
TaaI ACNGT 1 cut(s) 784
TaiI ACGT 2 cut(s) 14, 709
TaqI TCGA 1 cut(s) 855
TasI AATT 1 cut(s) 378
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 3 cut(s) 202, 806, 956
Tru1I TTAA 6 cut(s) 111, 377, 486, 566, 738, 752
Tru9I TTAA 6 cut(s) 111, 377, 486, 566, 738, 752
TscAI CASTG 1 cut(s) 721
TseFI GTSAC 2 cut(s) 133, 569
TseI GCWGC 7 cut(s) 39, 279, 388, 515, 545, 656, 989
Tsp45I GTSAC 2 cut(s) 133, 569
TspDTI ATGAA 2 cut(s) 417, 1025
TspGWI ACGGA 2 cut(s) 299, 760
TspRI CASTG 1 cut(s) 721
Van91I CCANNNNNTGG 1 cut(s) 144
Vha464I CTTAAG 1 cut(s) 565
XagI CCTNNNNNAGG 1 cut(s) 461
XbaI TCTAGA 1 cut(s) 397
XceI RCATGY 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 786
XmnI GAANNNNTTC 1 cut(s) 848
XspI CTAG 2 cut(s) 150, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.