AT5G35753

BEST Arabidopsis thaliana protein match is DNAJ heat shock N-terminal domain-containing protein (TAIR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010314
Physical Location & Seq
Reverse (-)
13922418 .. 13925454
3037 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35753.1

Sequence Viewer

Length: 1290 bp
ATGCCTACCAATTCCACTTATTTCTTAGGTCAGTATCCAGCACATGGATATGAATATGTTACAAATGAGTCATATGACTGGAGCTCATATGTGGGAACATCTCCTGGGAATCTGGAGTCAAACCGAATGTCTTCAGCATCCAACGGATATCCTTATAAGCTCAACAATGGAGTAGTCTTGATTAAAGCCAGCAGACCTGAGAAGAAGAGGAATGTTGGTTTAGGGTCATCTGGTAATGGATTTGTTGAGAACATTACAAAATCAAATCCAGAATCGAAAGCAACAAATTTGGATGCAAAGATGGAACATGACTTCAAACATCCTGGGAAGAGTTATGGTTTAATGAGACGGTGGTCTTCTACAACAGGATTGGATACACGGAAGATACTGATTCACAAAGCTAAAACAGATATTAAGCAAAGACTAGAGATTATGAGATTGGCTTCAGAAGCTGCTGCGACAGCTACAGAGGACGCGACTCCCCTTGATGAAGTAAGTGCGTCCTCCAAGGTGGGGGATGATGTATCCAGATTAGGAAAAAATGTTTCTTTTGGTCATCCACCTGTTCGGAAAATAAACGGGCCAATAACAGTTCCAGACTCCGACTTCCATGACTTTGATAAAAATAGATTAGAGGAGTGCTTCGAGGCTAGGCAAATATGGGCAATTTATGATGAAGATGATGGTATGCCACGCTTATATTGCATGGTCCGGGAAGTACTATCTGTTCAACCTTTTAAGATTGACATAGCCTACTTGAGCTCCAAAACTGACATCGAGTTTGGTACAATGAAATGGGTGCAGTATGGGTTTACAAAGTCGTGTGGACATTTCAGGATACGAAATACTGACATAGTTGATCATGTTAACATATTTTCCCATCTCTTAAAAGGCAAGAAAACAGGAAGAGGCGGTTGTGTTAGGATATTCCCCCAAACCGGAGATATTTGGACTGTGTACAAGAACTGGTCACCAAACTGGAACAACTCAACTCCAGATGAAGTGAGGCACCAATACAAAATGGTTGAGATCCTTGATGAATATTCTGAACAGTTTGGGGTTTGCATCGCCCCGCTTGTGAAAGTAGATGGATACAAAACCGTGTATTGCAGGAGAGATAAGGAGGAGAGCAAGAAATGGATACCGCGGAGAGAGATGCTGCGATTTTCGCATCAAGTGCCATCTCGGTTTCTTAAAGAAGAAACTTGTGGTGTGCCCGGAAACTGTTGGGACTTAGACCCATCTGCTATACCAGAAGAGTTGCTTCACAATGGAGCAGGAACTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

429

Amino Acids

48.67

Weight (kDa)

7.6

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 196 - 401 6.2e-74 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010314)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05230 AT2G05250 AT5G35753
fragaria_vesca FvH4_6g40880 FvH4_6g40880
malus_domestica MD09G1123800.v1.1 MD17G1114600.v1.1
prunus_persica Prupe.3G200300_v2.0.a1 Prupe.3G200300_v2.0.a1
pyrus_communis pycom09g04790 pycom17g10590
rosa_chinensis RchiOBHm_Chr2g0156451
rosa_laevigata RLG00000020913
rosa_multiflora Rmu_sc0005392.1_g000017
rosa_roxburghii Rroxscaffold_2G00093030
rosa_rugosa Rorug02G0453200
rosa_samantha Rh2AG519200 Rh2BG531500 Rh2CG504400 Rh2DG540500
rosa_wichuraiana Rw2G042820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 156
AccB1I GGYRCC 1 cut(s) 1008
AccB7I CCANNNNNTGG 1 cut(s) 44
AccII CGCG 2 cut(s) 476, 1147
AciI CCGC 4 cut(s) 912, 1073, 1145, 1147
AclWI GGATC 1 cut(s) 1024
AcsI RAATTY 1 cut(s) 286
AcuI CTGAAG 2 cut(s) 117, 429
AfaI GTAC 3 cut(s) 720, 787, 959
AfiI CCNNNNNNNGG 3 cut(s) 44, 513, 938
AgsI TTSAA 2 cut(s) 316, 731
AjnI CCWGG 2 cut(s) 103, 322
AluBI AGCT 6 cut(s) 84, 160, 401, 452, 464, 762
AluI AGCT 6 cut(s) 84, 160, 401, 452, 464, 762
Alw21I GWGCWC 2 cut(s) 86, 764
Alw26I GTCTC 1 cut(s) 340
AlwI GGATC 1 cut(s) 1024
AlwNI CAGNNNCTG 2 cut(s) 452, 1283
AoxI GGCC 1 cut(s) 581
ApeKI GCWGC 3 cut(s) 452, 455, 1159
ApoI RAATTY 1 cut(s) 286
ArsI GACNNNNNNTTYG 4 cut(s) 113, 145, 764, 796
Asp700I GAANNNNTTC 1 cut(s) 130
AspS9I GGNCC 2 cut(s) 581, 709
AsuC2I CCSGG 2 cut(s) 713, 1218
AsuHPI GGTGA 1 cut(s) 963
AvaII GGWCC 1 cut(s) 709
BaeGI GKGCMC 1 cut(s) 1218
BanI GGYRCC 1 cut(s) 1008
BanII GRGCYC 2 cut(s) 86, 764
BbsI GAAGAC 2 cut(s) 123, 348
Bbv12I GWGCWC 2 cut(s) 86, 764
BbvI GCAGC 3 cut(s) 439, 442, 1146
BccI CCATC 6 cut(s) 295, 677, 888, 1082, 1189, 1249
BciT130I CCWGG 2 cut(s) 105, 324
BciVI GTATCC 6 cut(s) 45, 367, 535, 831, 1085, 1134
BclI TGATCA 1 cut(s) 859
BcnI CCSGG 2 cut(s) 713, 1218
BcoDI GTCTC 1 cut(s) 340
BfaI CTAG 2 cut(s) 425, 651
BfmI CTRYAG 1 cut(s) 465
BfuI GTATCC 6 cut(s) 45, 367, 535, 831, 1085, 1134
BisI GCNGC 3 cut(s) 453, 456, 1160
BlsI GCNGC 3 cut(s) 454, 457, 1161
BmcAI AGTACT 1 cut(s) 720
Bme1390I CCNGG 4 cut(s) 105, 324, 713, 1218
Bme18I GGWCC 1 cut(s) 709
BmgT120I GGNCC 2 cut(s) 581, 709
BmiI GGNNCC 1 cut(s) 1010
BmrFI CCNGG 4 cut(s) 105, 324, 713, 1218
BmsI GCATC 5 cut(s) 146, 283, 1074, 1146, 1180
BoxI GACNNNNGTC 1 cut(s) 352
BpiI GAAGAC 2 cut(s) 123, 348
BpmI CTGGAG 3 cut(s) 100, 134, 978
BpuEI CTTGAG 1 cut(s) 778
BpuMI CCSGG 2 cut(s) 713, 1218
BsaJI CCNNGG 4 cut(s) 104, 323, 507, 1145
BsaWI WCCGGW 1 cut(s) 938
BsaXI ACNNNNNCTCC 2 cut(s) 746, 776
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 513, 938
Bse1I ACTGG 3 cut(s) 83, 971, 983
BseBI CCWGG 2 cut(s) 105, 324
BseDI CCNNGG 4 cut(s) 104, 323, 507, 1145
BseGI GGATG 5 cut(s) 137, 298, 319, 523, 556
BseLI CCNNNNNNNGG 3 cut(s) 44, 513, 938
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 3 cut(s) 83, 971, 983
BseRI GAGGAG 2 cut(s) 650, 1139
BseSI GKGCMC 1 cut(s) 1218
BseXI GCAGC 3 cut(s) 439, 442, 1146
BsgI GTGCAG 1 cut(s) 821
Bsh1236I CGCG 2 cut(s) 476, 1147
BshFI GGCC 1 cut(s) 583
BshNI GGYRCC 1 cut(s) 1008
BsiHKAI GWGCWC 2 cut(s) 86, 764
BsiSI CCGG 3 cut(s) 712, 939, 1218
BslFI GGGAC 1 cut(s) 1244
BslI CCNNNNNNNGG 3 cut(s) 44, 513, 938
BsmAI GTCTC 1 cut(s) 340
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 1 cut(s) 1244
BsnI GGCC 1 cut(s) 583
Bsp1286I GDGCHC 3 cut(s) 86, 764, 1218
Bsp1407I TGTACA 1 cut(s) 957
Bsp143I GATC 2 cut(s) 859, 1029
BspACI CCGC 4 cut(s) 912, 1073, 1145, 1147
BspANI GGCC 1 cut(s) 583
BspCNI CTCAG 1 cut(s) 190
BspFNI CGCG 2 cut(s) 476, 1147
BspLI GGNNCC 1 cut(s) 1010
BspPI GGATC 1 cut(s) 1024
BspT107I GGYRCC 1 cut(s) 1008
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 3 cut(s) 83, 971, 983
BssECI CCNNGG 4 cut(s) 104, 323, 507, 1145
BssMI GATC 2 cut(s) 859, 1029
BssT1I CCWWGG 1 cut(s) 507
Bst2UI CCWGG 2 cut(s) 105, 324
Bst4CI ACNGT 6 cut(s) 351, 592, 955, 1053, 1102, 1226
Bst6I CTCTTC 4 cut(s) 200, 323, 901, 1251
BstAPI GCANNNNNTGC 1 cut(s) 1177
BstAUI TGTACA 1 cut(s) 957
BstC8I GCNNGC 1 cut(s) 190
BstDEI CTNAG 3 cut(s) 25, 198, 1234
BstDSI CCRYGG 1 cut(s) 1145
BstEII GGTNACC 1 cut(s) 969
BstF5I GGATG 5 cut(s) 137, 298, 319, 523, 556
BstFNI CGCG 2 cut(s) 476, 1147
BstKTI GATC 2 cut(s) 862, 1032
BstMAI GTCTC 1 cut(s) 340
BstMBI GATC 2 cut(s) 859, 1029
BstMWI GCNNNNNNNGC 5 cut(s) 449, 461, 702, 1168, 1177
BstNI CCWGG 2 cut(s) 105, 324
BstPAI GACNNNNGTC 1 cut(s) 352
BstPI GGTNACC 1 cut(s) 969
BstSCI CCNGG 4 cut(s) 103, 322, 711, 1216
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 1 cut(s) 1218
BstUI CGCG 2 cut(s) 476, 1147
BstV1I GCAGC 3 cut(s) 439, 442, 1146
BstV2I GAAGAC 2 cut(s) 123, 348
BstX2I RGATCY 1 cut(s) 1029
BstYI RGATCY 1 cut(s) 1029
BsuI GTATCC 6 cut(s) 45, 367, 535, 831, 1085, 1134
BsuRI GGCC 1 cut(s) 583
BtgI CCRYGG 1 cut(s) 1145
BtgZI GCGATG 1 cut(s) 1051
BtsCI GGATG 5 cut(s) 137, 298, 319, 523, 556
Cac8I GCNNGC 1 cut(s) 190
CaiI CAGNNNCTG 2 cut(s) 452, 1283
Cfr13I GGNCC 2 cut(s) 581, 709
Cfr42I CCGCGG 1 cut(s) 1148
CseI GACGC 2 cut(s) 482, 489
Csp6I GTAC 3 cut(s) 719, 786, 958
CviAII CATG 5 cut(s) 44, 308, 611, 706, 863
CviQI GTAC 3 cut(s) 719, 786, 958
DdeI CTNAG 3 cut(s) 25, 198, 1234
DpnI GATC 2 cut(s) 861, 1031
DpnII GATC 2 cut(s) 859, 1029
Eam1104I CTCTTC 4 cut(s) 200, 323, 901, 1251
EarI CTCTTC 4 cut(s) 200, 323, 901, 1251
Ecl136II GAGCTC 2 cut(s) 84, 762
Eco130I CCWWGG 1 cut(s) 507
Eco24I GRGCYC 2 cut(s) 86, 764
Eco32I GATATC 1 cut(s) 149
Eco47I GGWCC 1 cut(s) 709
Eco53kI GAGCTC 2 cut(s) 84, 762
Eco57I CTGAAG 2 cut(s) 117, 429
Eco91I GGTNACC 1 cut(s) 969
EcoICRI GAGCTC 2 cut(s) 84, 762
EcoO65I GGTNACC 1 cut(s) 969
EcoRII CCWGG 2 cut(s) 103, 322
EcoRV GATATC 1 cut(s) 149
EcoT14I CCWWGG 1 cut(s) 507
EcoT38I GRGCYC 2 cut(s) 86, 764
ErhI CCWWGG 1 cut(s) 507
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 5 cut(s) 47, 311, 614, 709, 866
FalI AAGNNNNNCTT 2 cut(s) 1248, 1280
FaqI GGGAC 1 cut(s) 1244
FatI CATG 5 cut(s) 43, 307, 610, 705, 862
FauI CCCGC 1 cut(s) 1080
FauNDI CATATG 2 cut(s) 73, 88
FbaI TGATCA 1 cut(s) 859
Fnu4HI GCNGC 3 cut(s) 453, 456, 1160
FokI GGATG 5 cut(s) 124, 305, 306, 530, 543
FriOI GRGCYC 2 cut(s) 86, 764
Fsp4HI GCNGC 3 cut(s) 453, 456, 1160
FspBI CTAG 2 cut(s) 425, 651
GluI GCNGC 3 cut(s) 453, 456, 1160
GsuI CTGGAG 3 cut(s) 100, 134, 978
HaeIII GGCC 1 cut(s) 583
HapII CCGG 3 cut(s) 712, 939, 1218
HgaI GACGC 2 cut(s) 482, 489
Hin1II CATG 5 cut(s) 47, 311, 614, 709, 866
HincII GTYRAC 1 cut(s) 868
HindII GTYRAC 1 cut(s) 868
HinfI GANTC 7 cut(s) 68, 109, 116, 272, 391, 478, 599
HpaI GTTAAC 1 cut(s) 868
HpaII CCGG 3 cut(s) 712, 939, 1218
HphI GGTGA 1 cut(s) 963
Hpy166II GTNNAC 4 cut(s) 813, 827, 868, 958
Hpy188I TCNGA 4 cut(s) 448, 570, 604, 1048
Hpy188III TCNNGA 7 cut(s) 113, 178, 269, 528, 596, 835, 995
Hpy8I GTNNAC 4 cut(s) 813, 827, 868, 958
HpyCH4III ACNGT 6 cut(s) 351, 592, 955, 1053, 1102, 1226
HpyCH4V TGCA 5 cut(s) 296, 705, 802, 1065, 1110
HpyF10VI GCNNNNNNNGC 5 cut(s) 449, 461, 702, 1168, 1177
HpyF3I CTNAG 3 cut(s) 25, 198, 1234
Hsp92II CATG 5 cut(s) 47, 311, 614, 709, 866
Ksp22I TGATCA 1 cut(s) 859
KspAI GTTAAC 1 cut(s) 868
KspI CCGCGG 1 cut(s) 1148
Kzo9I GATC 2 cut(s) 859, 1029
LmnI GCTCC 3 cut(s) 81, 767, 1274
Lsp1109I GCAGC 3 cut(s) 439, 442, 1146
LweI GCATC 5 cut(s) 146, 283, 1074, 1146, 1180
MaeI CTAG 2 cut(s) 425, 651
MaeIII GTNAC 2 cut(s) 58, 969
MalI GATC 2 cut(s) 861, 1031
MboI GATC 2 cut(s) 859, 1029
MflI RGATCY 1 cut(s) 1029
MhlI GDGCHC 3 cut(s) 86, 764, 1218
MluCI AATT 3 cut(s) 10, 286, 666
MlyI GAGTC 4 cut(s) 77, 125, 472, 593
MmeI TCCRAC 2 cut(s) 165, 627
MnlI CCTC 8 cut(s) 201, 463, 514, 628, 640, 902, 999, 1117
MroXI GAANNNNTTC 1 cut(s) 130
MseI TTAA 7 cut(s) 183, 341, 414, 738, 867, 887, 1194
MslI CAYNNNNRTG 1 cut(s) 48
MspA1I CMGCKG 1 cut(s) 1147
MspI CCGG 3 cut(s) 712, 939, 1218
MspR9I CCNGG 4 cut(s) 105, 324, 713, 1218
MvaI CCWGG 2 cut(s) 105, 324
MvnI CGCG 2 cut(s) 476, 1147
MwoI GCNNNNNNNGC 5 cut(s) 449, 461, 702, 1168, 1177
NciI CCSGG 2 cut(s) 713, 1218
NdeI CATATG 2 cut(s) 73, 88
NdeII GATC 2 cut(s) 859, 1029
NlaIII CATG 5 cut(s) 47, 311, 614, 709, 866
NlaIV GGNNCC 1 cut(s) 1010
NmuCI GTSAC 1 cut(s) 969
PdmI GAANNNNTTC 1 cut(s) 130
PfeI GAWTC 3 cut(s) 109, 272, 391
PflMI CCANNNNNTGG 1 cut(s) 44
PfoI TCCNGGA 1 cut(s) 711
PkrI GCNGC 3 cut(s) 454, 457, 1161
PleI GAGTC 4 cut(s) 76, 124, 472, 593
PpsI GAGTC 4 cut(s) 76, 124, 472, 593
PshAI GACNNNNGTC 1 cut(s) 352
PsiI TTATAA 1 cut(s) 156
Psp124BI GAGCTC 2 cut(s) 86, 764
Psp6I CCWGG 2 cut(s) 103, 322
PspEI GGTNACC 1 cut(s) 969
PspGI CCWGG 2 cut(s) 103, 322
PspN4I GGNNCC 1 cut(s) 1010
PspPI GGNCC 2 cut(s) 581, 709
PsrI GAACNNNNNNTAC 2 cut(s) 711, 743
PstNI CAGNNNCTG 2 cut(s) 452, 1283
PsuI RGATCY 1 cut(s) 1029
RsaI GTAC 3 cut(s) 720, 787, 959
RsaNI GTAC 3 cut(s) 719, 786, 958
RseI CAYNNNNRTG 1 cut(s) 48
SacI GAGCTC 2 cut(s) 86, 764
SacII CCGCGG 1 cut(s) 1148
SaqAI TTAA 7 cut(s) 183, 341, 414, 738, 867, 887, 1194
SatI GCNGC 3 cut(s) 453, 456, 1160
Sau3AI GATC 2 cut(s) 859, 1029
Sau96I GGNCC 2 cut(s) 581, 709
ScaI AGTACT 1 cut(s) 720
SchI GAGTC 4 cut(s) 77, 125, 472, 593
ScrFI CCNGG 4 cut(s) 105, 324, 713, 1218
SduI GDGCHC 3 cut(s) 86, 764, 1218
SfaNI GCATC 5 cut(s) 146, 283, 1074, 1146, 1180
SfcI CTRYAG 1 cut(s) 465
Sfr303I CCGCGG 1 cut(s) 1148
SgrBI CCGCGG 1 cut(s) 1148
SinI GGWCC 1 cut(s) 709
SmiMI CAYNNNNRTG 1 cut(s) 48
SmlI CTYRAG 1 cut(s) 757
SmoI CTYRAG 1 cut(s) 757
Sse9I AATT 3 cut(s) 10, 286, 666
SsiI CCGC 4 cut(s) 912, 1073, 1145, 1147
SspI AATATT 1 cut(s) 1043
SspMI CTAG 2 cut(s) 425, 651
SstI GAGCTC 2 cut(s) 86, 764
StyD4I CCNGG 4 cut(s) 103, 322, 711, 1216
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 6 cut(s) 351, 592, 955, 1053, 1102, 1226
TaqI TCGA 3 cut(s) 275, 645, 777
TasI AATT 3 cut(s) 10, 286, 666
TatI WGTACW 2 cut(s) 718, 957
TfiI GAWTC 3 cut(s) 109, 272, 391
Tru1I TTAA 7 cut(s) 183, 341, 414, 738, 867, 887, 1194
Tru9I TTAA 7 cut(s) 183, 341, 414, 738, 867, 887, 1194
TseFI GTSAC 1 cut(s) 969
TseI GCWGC 3 cut(s) 452, 455, 1159
Tsp45I GTSAC 1 cut(s) 969
TspDTI ATGAA 6 cut(s) 66, 504, 690, 806, 1014, 1053
TspGWI ACGGA 2 cut(s) 159, 394
Van91I CCANNNNNTGG 1 cut(s) 44
VpaK11BI GGWCC 1 cut(s) 709
XapI RAATTY 1 cut(s) 286
XmnI GAANNNNTTC 1 cut(s) 130
XspI CTAG 2 cut(s) 425, 651
ZrmI AGTACT 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.