MD17G1114600.v1.1

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010314
Physical Location & Seq
Reverse (-)
9815906 .. 9819151
3246 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1114600.v1.1.491

Sequence Viewer

Length: 2103 bp
ATGGAAGTTAACATAGAGGAGGCTCTTAAAGCGAAAGAGACCGCTGAGAAGCGATTTTTTGAGAAAGATTTTACAGGTGCTCTCACTTATGCAGTAAAAGCTAAGTCACTGTACCCTGAACTTGAGGGCATATCTCATATGCTAGCCACATTTGACGTTTATGTTGCTTCTGAGGCAAAATATTATGGTGAAATGGATCATTATAAAATTCTTGGACTGAAGCATTCTGCAAGTAAAGATGCAGTGAAGAAGCAGTACAAAAAGATGGCTGTGTTGCTCCACCCTGATAAGAACAAATGCGTGGGAGCTGATGGGGCTTTCAAACTTGTTTCTGAAGCAGTTACTGTGTTGTCCGACACTGCCAAGAGAAGGTTGTATGATCTTCAGAGAAACAAACAATTAGCAGCTACAGGTAATCAGCCCAATTTGTCTTCAGTTCACGCTGGTGGGGTTCAAGGTTTCAACAATTGTTCCAGTTCATCAGTATCTCATGGTAGACTTGACACTTTCTGGACCGTTTGCACTTCTTGCAAGGTTCAGTATGAGTATCTCCGCAAGTATGTGAACAAAAGACTTTCATGTAAAAACTGCCGAGGCATTTTCATTGCTGTGGAATCTGGGGCAGCCCCAGCAAATGGTTCTTTTCCTTATACTCCTTGTCCTTGGCCGTATGTGCCTAGTAATGGGTATGGGAATCATGGATTTGATGGGGTTACATATGTTCCAAGCAACGGTACCTTTTTCCCAGGAAATGGGATCTCAGGTTTTCATTCTGGACATGGGTATGAATATACTTCAAATATGTCGTTTCAGTGGAACTCATATTCTGGAACCTCAGCTGGTGTTGCAGCTCTTCATGGGTCATCGGCTATATCCCCTGATGCTGTTTATCAGTCAAATGGAAATGTTAAGAAAGCTAGAGAGAAGGTGAAATCAAGAGTGAATGGAAAACGTTCTGTTACAAATGTTGTGGCTAAAGTGGATCTGACCGTGCCTGCTAGCTGTAATCAGCCACCTGAATCTAAGAGCAATGGACGGGATAAGAGAAGGAAACTTACTGTGGGAGCGAGTTTTAGAAATGGGCTTGATGAAAAAGTATCAAAATCTGCTTTAGAAGCAAAACTAGCCAATGGAAATGGGAGTATTGGAAGTGATCAGAAAATTCTTAGTTCAAGGGAACTTCCAACTAGACGTTGCTCCATGGCACCGGCTTTTGATACGAAGAAGTTGTTGCTTGAAAAGGCAAGAACTGAAATTTTGAAGAAATTGGCTGAGTTTAAGTTGGCATCAGAAGCAGCAGCTGCTGTAAAGAACACAGAAAGAGAGGCTGAAGTGCGTCAACCAGAAGTTGCACAAGCACAGAAATTAGAGATAAAGAATACTGAGCCCCTCTGCATAACTGTCCCTGACCCCGACTTCCATGATTTTGATAAGGATAGGTCAGAGGAATCTTTCAAGCCAAAGCAAATTTGGGCTTTGTATGATGAAGAAGATGGTATGCCTCGCCTGTACTGTCTGATTCGTGAAGTGGTTTCGCTAAAACCATTCAAGTTAGTCATCACTTACTTGGGCTCTAAAACTGATAGTGAATTCGGGTCCGTGAACTGGCTAGATTGTGGGTTTACCAAATCCTGTGGAAATTTTAGGGCACGGAATCTTGATGTCATTGAGCATGTTAACATTTTCTCTCATGCTTTGAGCCGGGAGAAAGCAGGCAGAGGAGGCTGTGTTCGGATATACCCCAGAAGCGGGGACATTTGGGCTATGTATCGAAACTGGTCAAAAAATTGGGACAGATCGACTCCAGCTGAAGTGAGGCACCAGTACATAATGGTGGAAGTTCTTGATGATTACTCCGAAGATCTTGGTGTCTGCGTATCCCCTCTTGTGAAACTGGCTGGATTCAAGACAGTATACCAAAGCAATACGGACAAAGCGGCCATCCAATGGATTCCAAAAAGAGAGATGCTACGCTTCTCGCACCAGGTGCCATCGTGGTTACTCAAGGGAGAAGAGAGTAACCTGCCCGGTAAGTGCTGGGATCTTGATCCAGCTGCAACTCCCGACGAGCTTCTTCACGCCGCGGTGGAAACCGAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

701

Amino Acids

77.68

Weight (kDa)

8.88

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 66 - 127 2.1e-20 DnaJ domain
Zn_ribbon_20 PF23551 170 - 204 9.5e-14 Zinc beta-ribbon domain
DUF3444 PF11926 465 - 672 1.6e-74 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010314)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05230 AT2G05250 AT5G35753
fragaria_vesca FvH4_6g40880 FvH4_6g40880
malus_domestica MD09G1123800.v1.1 MD17G1114600.v1.1
prunus_persica Prupe.3G200300_v2.0.a1 Prupe.3G200300_v2.0.a1
pyrus_communis pycom09g04790 pycom17g10590
rosa_chinensis RchiOBHm_Chr2g0156451
rosa_laevigata RLG00000020913
rosa_multiflora Rmu_sc0005392.1_g000017
rosa_roxburghii Rroxscaffold_2G00093030
rosa_rugosa Rorug02G0453200
rosa_samantha Rh2AG519200 Rh2BG531500 Rh2CG504400 Rh2DG540500
rosa_wichuraiana Rw2G042820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 204
Acc36I ACCTGC 1 cut(s) 2031
Acc65I GGTACC 1 cut(s) 734
AccB1I GGYRCC 4 cut(s) 734, 1204, 1818, 1987
AccB7I CCANNNNNTGG 3 cut(s) 635, 752, 1947
AccI GTMKAC 2 cut(s) 496, 1914
AccII CGCG 1 cut(s) 2084
AciI CCGC 6 cut(s) 42, 553, 1749, 1937, 2082, 2084
AclI AACGTT 1 cut(s) 952
AclWI GGATC 5 cut(s) 204, 764, 990, 2042, 2049
AcoI YGGCCR 2 cut(s) 665, 1938
AcsI RAATTY 6 cut(s) 207, 1161, 1254, 1467, 1589, 1639
AcuI CTGAAG 6 cut(s) 239, 354, 368, 417, 1350, 1830
AdeI CACNNNGTG 1 cut(s) 1987
AfaI GTAC 5 cut(s) 113, 257, 736, 1511, 1826
AfiI CCNNNNNNNGG 9 cut(s) 369, 635, 683, 731, 752, 1605, 1748, 1749, 1947
AjnI CCWGG 2 cut(s) 745, 1983
AleI CACNNNNGTG 1 cut(s) 444
AloI GAACNNNNNNTCC 2 cut(s) 1713, 1745
Alw21I GWGCWC 1 cut(s) 82
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 5 cut(s) 204, 764, 990, 2042, 2049
AlwNI CAGNNNCTG 3 cut(s) 344, 1301, 1304
AoxI GGCC 2 cut(s) 665, 1938
ApeKI GCWGC 7 cut(s) 404, 623, 848, 1295, 1298, 1301, 2054
ApoI RAATTY 6 cut(s) 207, 1161, 1254, 1467, 1589, 1639
Asp700I GAANNNNTTC 1 cut(s) 952
Asp718I GGTACC 1 cut(s) 734
AspS9I GGNCC 2 cut(s) 513, 1596
AsuC2I CCSGG 2 cut(s) 1703, 2028
AsuHPI GGTGA 2 cut(s) 200, 940
AsuNHI GCTAGC 2 cut(s) 142, 998
AvaII GGWCC 2 cut(s) 513, 1596
BaeGI GKGCMC 1 cut(s) 1651
BanI GGYRCC 4 cut(s) 734, 1204, 1818, 1987
BanII GRGCYC 2 cut(s) 1389, 1574
BarI GAAGNNNNNNTAC 4 cut(s) 239, 271, 1039, 1071
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 82
BbvCI CCTCAGC 1 cut(s) 835
BbvI GCAGC 7 cut(s) 416, 635, 860, 1288, 1307, 1310, 2041
BccI CCATC 6 cut(s) 259, 305, 701, 1487, 1949, 1999
BceAI ACGGC 1 cut(s) 652
BciT130I CCWGG 2 cut(s) 747, 1985
BciVI GTATCC 1 cut(s) 1888
BclI TGATCA 1 cut(s) 1153
BcnI CCSGG 2 cut(s) 1703, 2028
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 7 cut(s) 143, 678, 918, 999, 1124, 1188, 1610
BfmI CTRYAG 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 2031
BfuI GTATCC 1 cut(s) 1888
BglII AGATCT 1 cut(s) 1861
BisI GCNGC 9 cut(s) 405, 624, 849, 1296, 1299, 1302, 1938, 2055, 2082
BlsI GCNGC 9 cut(s) 406, 625, 850, 1297, 1300, 1303, 1939, 2056, 2083
Bme1390I CCNGG 4 cut(s) 747, 1703, 1985, 2028
Bme18I GGWCC 2 cut(s) 513, 1596
BmgT120I GGNCC 2 cut(s) 513, 1596
BmiI GGNNCC 6 cut(s) 736, 832, 1206, 1597, 1820, 1989
BmrFI CCNGG 4 cut(s) 747, 1703, 1985, 2028
BmsI GCATC 4 cut(s) 229, 871, 1295, 1956
BmtI GCTAGC 2 cut(s) 146, 1002
BpiI GAAGAC 1 cut(s) 423
BpmI CTGGAG 1 cut(s) 1788
Bpu10I CCTNAGC 1 cut(s) 835
BpuEI CTTGAG 2 cut(s) 143, 1988
BpuMI CCSGG 2 cut(s) 1703, 2028
BsaBI GATNNNNATC 1 cut(s) 2046
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 5 cut(s) 592, 662, 745, 1200, 2082
BsaXI ACNNNNNCTCC 2 cut(s) 1713, 1743
Bsc4I CCNNNNNNNGG 9 cut(s) 369, 635, 683, 731, 752, 1605, 1748, 1749, 1947
Bse118I RCCGGY 1 cut(s) 1207
Bse1I ACTGG 5 cut(s) 474, 1610, 1781, 1822, 1899
Bse3DI GCAATG 2 cut(s) 603, 1036
Bse8I GATNNNNATC 1 cut(s) 2046
BseBI CCWGG 2 cut(s) 747, 1985
BseDI CCNNGG 5 cut(s) 592, 662, 745, 1200, 2082
BseGI GGATG 1 cut(s) 1941
BseJI GATNNNNATC 1 cut(s) 2046
BseLI CCNNNNNNNGG 9 cut(s) 369, 635, 683, 731, 752, 1605, 1748, 1749, 1947
BseMI GCAATG 2 cut(s) 603, 1036
BseMII CTCAG 6 cut(s) 36, 162, 774, 849, 1263, 1374
BseNI ACTGG 5 cut(s) 474, 1610, 1781, 1822, 1899
BseRI GAGGAG 2 cut(s) 32, 1734
BseSI GKGCMC 1 cut(s) 1651
BseXI GCAGC 7 cut(s) 416, 635, 860, 1288, 1307, 1310, 2041
BseYI CCCAGC 2 cut(s) 628, 2037
Bsh1236I CGCG 1 cut(s) 2084
BshFI GGCC 2 cut(s) 667, 1940
BshNI GGYRCC 4 cut(s) 734, 1204, 1818, 1987
BsiHKAI GWGCWC 1 cut(s) 82
BsiSI CCGG 3 cut(s) 1208, 1702, 2028
BslFI GGGAC 3 cut(s) 1388, 1766, 1805
BslI CCNNNNNNNGG 9 cut(s) 369, 635, 683, 731, 752, 1605, 1748, 1749, 1947
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 3 cut(s) 1388, 1766, 1805
BsmI GAATGC 1 cut(s) 223
BsnI GGCC 2 cut(s) 667, 1940
Bso31I GGTCTC 1 cut(s) 32
Bsp1286I GDGCHC 4 cut(s) 82, 1389, 1574, 1651
Bsp143I GATC 9 cut(s) 196, 379, 756, 982, 1153, 1796, 1861, 2041, 2047
Bsp19I CCATGG 1 cut(s) 1200
BspACI CCGC 6 cut(s) 42, 553, 1749, 1937, 2082, 2084
BspANI GGCC 2 cut(s) 667, 1940
BspCNI CTCAG 6 cut(s) 37, 163, 773, 848, 1264, 1375
BspFNI CGCG 1 cut(s) 2084
BspLI GGNNCC 6 cut(s) 736, 832, 1206, 1597, 1820, 1989
BspMI ACCTGC 1 cut(s) 2031
BspOI GCTAGC 2 cut(s) 146, 1002
BspPI GGATC 5 cut(s) 204, 764, 990, 2042, 2049
BspQI GCTCTTC 1 cut(s) 858
BspT107I GGYRCC 4 cut(s) 734, 1204, 1818, 1987
BspTNI GGTCTC 1 cut(s) 32
BsrDI GCAATG 2 cut(s) 603, 1036
BsrFI RCCGGY 1 cut(s) 1207
BsrI ACTGG 5 cut(s) 474, 1610, 1781, 1822, 1899
BssAI RCCGGY 1 cut(s) 1207
BssECI CCNNGG 5 cut(s) 592, 662, 745, 1200, 2082
BssMI GATC 9 cut(s) 196, 379, 756, 982, 1153, 1796, 1861, 2041, 2047
BssNAI GTATAC 1 cut(s) 1915
BssT1I CCWWGG 2 cut(s) 662, 1200
Bst1107I GTATAC 1 cut(s) 1915
Bst2UI CCWGG 2 cut(s) 747, 1985
Bst4CI ACNGT 9 cut(s) 111, 346, 517, 734, 991, 1060, 1402, 1514, 1912
Bst6I CTCTTC 2 cut(s) 858, 2007
BstAPI GCANNNNNTGC 3 cut(s) 528, 1301, 1987
BstC8I GCNNGC 4 cut(s) 144, 996, 1000, 1714
BstDEI CTNAG 9 cut(s) 45, 102, 171, 760, 835, 1023, 1166, 1272, 1383
BstDSI CCRYGG 2 cut(s) 1200, 2082
BstF5I GGATG 1 cut(s) 1941
BstFNI CGCG 1 cut(s) 2084
BstKTI GATC 9 cut(s) 199, 382, 759, 985, 1156, 1799, 1864, 2044, 2050
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 9 cut(s) 196, 379, 756, 982, 1153, 1796, 1861, 2041, 2047
BstNI CCWGG 2 cut(s) 747, 1985
BstNSI RCATGY 1 cut(s) 1676
BstSCI CCNGG 4 cut(s) 745, 1701, 1983, 2026
BstSFI CTRYAG 1 cut(s) 408
BstSLI GKGCMC 1 cut(s) 1651
BstUI CGCG 1 cut(s) 2084
BstV1I GCAGC 7 cut(s) 416, 635, 860, 1288, 1307, 1310, 2041
BstV2I GAAGAC 1 cut(s) 423
BstX2I RGATCY 4 cut(s) 756, 982, 1861, 2041
BstYI RGATCY 4 cut(s) 756, 982, 1861, 2041
BstZ17I GTATAC 1 cut(s) 1915
BsuI GTATCC 1 cut(s) 1888
BsuRI GGCC 2 cut(s) 667, 1940
BtgI CCRYGG 2 cut(s) 1200, 2082
BtsCI GGATG 1 cut(s) 1941
BtsI GCAGTG 2 cut(s) 249, 357
BtsIMutI CAGTG 4 cut(s) 107, 249, 357, 818
BveI ACCTGC 1 cut(s) 2031
Cac8I GCNNGC 4 cut(s) 144, 996, 1000, 1714
CaiI CAGNNNCTG 3 cut(s) 344, 1301, 1304
Cfr10I RCCGGY 1 cut(s) 1207
Cfr13I GGNCC 2 cut(s) 513, 1596
Cfr42I CCGCGG 1 cut(s) 2085
CseI GACGC 1 cut(s) 1325
CsiI ACCWGGT 1 cut(s) 1983
Csp6I GTAC 5 cut(s) 112, 256, 735, 1510, 1825
CspCI CAANNNNNGTGG 4 cut(s) 951, 986, 1615, 1650
CviAII CATG 9 cut(s) 491, 579, 698, 779, 857, 1201, 1421, 1673, 1691
CviQI GTAC 5 cut(s) 112, 256, 735, 1510, 1825
DdeI CTNAG 9 cut(s) 45, 102, 171, 760, 835, 1023, 1166, 1272, 1383
DpnI GATC 9 cut(s) 198, 381, 758, 984, 1155, 1798, 1863, 2043, 2049
DpnII GATC 9 cut(s) 196, 379, 756, 982, 1153, 1796, 1861, 2041, 2047
DraIII CACNNNGTG 1 cut(s) 1987
EaeI YGGCCR 2 cut(s) 665, 1938
Eam1104I CTCTTC 2 cut(s) 858, 2007
EarI CTCTTC 2 cut(s) 858, 2007
Eco130I CCWWGG 2 cut(s) 662, 1200
Eco24I GRGCYC 2 cut(s) 1389, 1574
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 2 cut(s) 513, 1596
Eco57I CTGAAG 6 cut(s) 239, 354, 368, 417, 1350, 1830
EcoRI GAATTC 1 cut(s) 1589
EcoRII CCWGG 2 cut(s) 745, 1983
EcoT14I CCWWGG 2 cut(s) 662, 1200
EcoT38I GRGCYC 2 cut(s) 1389, 1574
ErhI CCWWGG 2 cut(s) 662, 1200
FaeI CATG 9 cut(s) 494, 582, 701, 782, 860, 1204, 1424, 1676, 1694
FaqI GGGAC 3 cut(s) 1388, 1766, 1805
FatI CATG 9 cut(s) 490, 578, 697, 778, 856, 1200, 1420, 1672, 1690
FauI CCCGC 1 cut(s) 1742
FauNDI CATATG 2 cut(s) 138, 718
FbaI TGATCA 1 cut(s) 1153
FblI GTMKAC 2 cut(s) 496, 1914
Fnu4HI GCNGC 9 cut(s) 405, 624, 849, 1296, 1299, 1302, 1938, 2055, 2082
FokI GGATG 1 cut(s) 1928
FriOI GRGCYC 2 cut(s) 1389, 1574
Fsp4HI GCNGC 9 cut(s) 405, 624, 849, 1296, 1299, 1302, 1938, 2055, 2082
FspBI CTAG 7 cut(s) 143, 678, 918, 999, 1124, 1188, 1610
GluI GCNGC 9 cut(s) 405, 624, 849, 1296, 1299, 1302, 1938, 2055, 2082
GsaI CCCAGC 2 cut(s) 632, 2041
GsuI CTGGAG 1 cut(s) 1788
HaeIII GGCC 2 cut(s) 667, 1940
HapII CCGG 3 cut(s) 1208, 1702, 2028
HgaI GACGC 1 cut(s) 1325
Hin1II CATG 9 cut(s) 494, 582, 701, 782, 860, 1204, 1424, 1676, 1694
HincII GTYRAC 3 cut(s) 10, 1340, 1678
HindII GTYRAC 3 cut(s) 10, 1340, 1678
HindIII AAGCTT 1 cut(s) 2097
HinfI GANTC 9 cut(s) 614, 694, 1019, 1448, 1519, 1654, 1801, 1902, 1951
HpaI GTTAAC 2 cut(s) 10, 1678
HpaII CCGG 3 cut(s) 1208, 1702, 2028
HphI GGTGA 2 cut(s) 200, 940
Hpy166II GTNNAC 9 cut(s) 10, 439, 497, 565, 1340, 1603, 1623, 1678, 1915
Hpy8I GTNNAC 9 cut(s) 10, 439, 497, 565, 1340, 1603, 1623, 1678, 1915
Hpy99I CGWCG 1 cut(s) 2069
HpyAV CCTTC 3 cut(s) 363, 919, 1041
HpyCH4III ACNGT 9 cut(s) 111, 346, 517, 734, 991, 1060, 1402, 1514, 1912
HpyCH4IV ACGT 3 cut(s) 156, 952, 1192
HpyCH4V TGCA 9 cut(s) 92, 230, 242, 522, 531, 848, 1352, 1395, 2057
HpyF3I CTNAG 9 cut(s) 45, 102, 171, 760, 835, 1023, 1166, 1272, 1383
HpySE526I ACGT 3 cut(s) 156, 952, 1192
Hsp92II CATG 9 cut(s) 494, 582, 701, 782, 860, 1204, 1424, 1676, 1694
KpnI GGTACC 1 cut(s) 738
Ksp22I TGATCA 1 cut(s) 1153
KspAI GTTAAC 2 cut(s) 10, 1678
KspI CCGCGG 1 cut(s) 2085
Kzo9I GATC 9 cut(s) 196, 379, 756, 982, 1153, 1796, 1861, 2041, 2047
LguI GCTCTTC 1 cut(s) 858
LmnI GCTCC 4 cut(s) 282, 305, 1064, 1202
Lsp1109I GCAGC 7 cut(s) 416, 635, 860, 1288, 1307, 1310, 2041
LweI GCATC 4 cut(s) 229, 871, 1295, 1956
MabI ACCWGGT 1 cut(s) 1983
MaeI CTAG 7 cut(s) 143, 678, 918, 999, 1124, 1188, 1610
MaeII ACGT 3 cut(s) 156, 952, 1192
MaeIII GTNAC 6 cut(s) 105, 340, 712, 958, 1998, 2018
MalI GATC 9 cut(s) 198, 381, 758, 984, 1155, 1798, 1863, 2043, 2049
MboI GATC 9 cut(s) 196, 379, 756, 982, 1153, 1796, 1861, 2041, 2047
MfeI CAATTG 1 cut(s) 466
MflI RGATCY 4 cut(s) 756, 982, 1861, 2041
MhlI GDGCHC 4 cut(s) 82, 1389, 1574, 1651
MlyI GAGTC 1 cut(s) 1795
MmeI TCCRAC 2 cut(s) 378, 1208
MroXI GAANNNNTTC 1 cut(s) 952
MseI TTAA 6 cut(s) 9, 27, 909, 1278, 1677, 2101
MslI CAYNNNNRTG 4 cut(s) 444, 608, 783, 1832
MspA1I CMGCKG 6 cut(s) 44, 839, 1301, 1808, 2054, 2084
MspI CCGG 3 cut(s) 1208, 1702, 2028
MspR9I CCNGG 4 cut(s) 747, 1703, 1985, 2028
MunI CAATTG 1 cut(s) 466
Mva1269I GAATGC 1 cut(s) 223
MvaI CCWGG 2 cut(s) 747, 1985
MvnI CGCG 1 cut(s) 2084
NciI CCSGG 2 cut(s) 1703, 2028
NcoI CCATGG 1 cut(s) 1200
NdeI CATATG 2 cut(s) 138, 718
NdeII GATC 9 cut(s) 196, 379, 756, 982, 1153, 1796, 1861, 2041, 2047
NheI GCTAGC 2 cut(s) 142, 998
NlaIII CATG 9 cut(s) 494, 582, 701, 782, 860, 1204, 1424, 1676, 1694
NlaIV GGNNCC 6 cut(s) 736, 832, 1206, 1597, 1820, 1989
NmeAIII GCCGAG 1 cut(s) 617
NmuCI GTSAC 1 cut(s) 105
NspI RCATGY 1 cut(s) 1676
OliI CACNNNNGTG 1 cut(s) 444
PciSI GCTCTTC 1 cut(s) 858
PctI GAATGC 1 cut(s) 223
PdmI GAANNNNTTC 1 cut(s) 952
PfeI GAWTC 8 cut(s) 614, 694, 1019, 1448, 1519, 1654, 1902, 1951
PflMI CCANNNNNTGG 3 cut(s) 635, 752, 1947
PkrI GCNGC 9 cut(s) 406, 625, 850, 1297, 1300, 1303, 1939, 2056, 2083
PleI GAGTC 1 cut(s) 1795
PpsI GAGTC 1 cut(s) 1795
PsiI TTATAA 1 cut(s) 204
Psp1406I AACGTT 1 cut(s) 952
Psp6I CCWGG 2 cut(s) 745, 1983
PspFI CCCAGC 2 cut(s) 628, 2037
PspGI CCWGG 2 cut(s) 745, 1983
PspN4I GGNNCC 6 cut(s) 736, 832, 1206, 1597, 1820, 1989
PspPI GGNCC 2 cut(s) 513, 1596
PstNI CAGNNNCTG 3 cut(s) 344, 1301, 1304
PsuI RGATCY 4 cut(s) 756, 982, 1861, 2041
PvuII CAGCTG 4 cut(s) 839, 1301, 1808, 2054
RsaI GTAC 5 cut(s) 113, 257, 736, 1511, 1826
RsaNI GTAC 5 cut(s) 112, 256, 735, 1510, 1825
RseI CAYNNNNRTG 4 cut(s) 444, 608, 783, 1832
SacII CCGCGG 1 cut(s) 2085
SapI GCTCTTC 1 cut(s) 858
SaqAI TTAA 6 cut(s) 9, 27, 909, 1278, 1677, 2101
SatI GCNGC 9 cut(s) 405, 624, 849, 1296, 1299, 1302, 1938, 2055, 2082
Sau3AI GATC 9 cut(s) 196, 379, 756, 982, 1153, 1796, 1861, 2041, 2047
Sau96I GGNCC 2 cut(s) 513, 1596
SchI GAGTC 1 cut(s) 1795
ScrFI CCNGG 4 cut(s) 747, 1703, 1985, 2028
SduI GDGCHC 4 cut(s) 82, 1389, 1574, 1651
SexAI ACCWGGT 1 cut(s) 1983
SfaNI GCATC 4 cut(s) 229, 871, 1295, 1956
SfcI CTRYAG 1 cut(s) 408
Sfr303I CCGCGG 1 cut(s) 2085
SgrBI CCGCGG 1 cut(s) 2085
SinI GGWCC 2 cut(s) 513, 1596
SmiMI CAYNNNNRTG 4 cut(s) 444, 608, 783, 1832
SmlI CTYRAG 2 cut(s) 122, 2003
SmoI CTYRAG 2 cut(s) 122, 2003
SsiI CCGC 6 cut(s) 42, 553, 1749, 1937, 2082, 2084
SspI AATATT 1 cut(s) 182
SspMI CTAG 7 cut(s) 143, 678, 918, 999, 1124, 1188, 1610
StyD4I CCNGG 4 cut(s) 745, 1701, 1983, 2026
StyI CCWWGG 2 cut(s) 662, 1200
TaaI ACNGT 9 cut(s) 111, 346, 517, 734, 991, 1060, 1402, 1514, 1912
TaiI ACGT 3 cut(s) 159, 955, 1195
TaqI TCGA 2 cut(s) 1771, 1799
TatI WGTACW 3 cut(s) 255, 1509, 1824
TauI GCSGC 2 cut(s) 1940, 2084
TfiI GAWTC 8 cut(s) 614, 694, 1019, 1448, 1519, 1654, 1902, 1951
Tru1I TTAA 6 cut(s) 9, 27, 909, 1278, 1677, 2101
Tru9I TTAA 6 cut(s) 9, 27, 909, 1278, 1677, 2101
TscAI CASTG 4 cut(s) 114, 249, 364, 818
TseFI GTSAC 1 cut(s) 105
TseI GCWGC 7 cut(s) 404, 623, 848, 1295, 1298, 1301, 2054
Tsp45I GTSAC 1 cut(s) 105
TspDTI ATGAA 8 cut(s) 468, 567, 592, 758, 801, 845, 1104, 1500
TspGWI ACGGA 3 cut(s) 1588, 1666, 1943
TspRI CASTG 4 cut(s) 114, 249, 364, 818
Van91I CCANNNNNTGG 3 cut(s) 635, 752, 1947
VpaK11BI GGWCC 2 cut(s) 513, 1596
XapI RAATTY 6 cut(s) 207, 1161, 1254, 1467, 1589, 1639
XceI RCATGY 1 cut(s) 1676
XcmI CCANNNNNNNNNTGG 1 cut(s) 1467
XmiI GTMKAC 2 cut(s) 496, 1914
XmnI GAANNNNTTC 1 cut(s) 952
XspI CTAG 7 cut(s) 143, 678, 918, 999, 1124, 1188, 1610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.