Rh2AG519200

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010314
Physical Location & Seq
Forward (+)
74690486 .. 74694467
3982 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG519200.1

Sequence Viewer

Length: 2343 bp
ATGGAAGTTAACAAAGAGGAGGCCCTTAAGGCGAAGGAGATCGCTGAGAAGCGATTTGCTGACAAGGACTATATAAATGCTAAGAATTATGCAGTGAAGGCGAAATCACTCAATCCGGAATTGGAGGGCATAGCTCAAATGGTTGCCACATTTGAAGTTTATGCCGCTACCGAGTACAGATTCTGTGGTGAAATAGATCATTATTCGATTCTTGGGTTGAAGCCCACCTCAAGTAAAGATGCAGTGAAGAAGCAGTATAAGAAGATGGCTGTGTTGCTCCACCCTGATAAGAACAAATGTGTGGGAGCTGATGGCGCATTCAAACTTGTCTCTGAAGCATGGACCTTGTTGTCTGACACTACCAAGAGAGGCTTTTATGATCTTCAAAGAAACAAGCACTTGGCAACTGCAGTTAATCAACCAAATTACTCTACAGTTCATGCTGGTGGGGTTATGACATCTCATGGTATACTTGACACTTTCTGGACCGTTTGCACTTCTTGCAAGGTTCAGTATGAGTATCTCCGGAAATATGTCAACAAAAGACTTTCGTGTAAGAACTGTCGAGGTGTTTTCATTGCTGTGGAATCTGGGGCAGCCCCAGCAAATGGCTCCTTCCCCTATACTCCTTGGCCATATCCTAATAATGGGCATTCAAATCATGGGGTTACCTATGTTCCAAACAATGGTACCTTGTTCCCAGCAAATGGTGCCCCTGGATTTCATACTGGACATGCATATGACTATATTTCAAATGTGTCATTTCAGTGGAGCTCATATTCAGGAACTTCTGCTGGAGTTGTCAGCCCTCACGGGTCCTCATCAGCTGTAACCTCTGATGTTGTTTATCAGGCAAATGGAAAGGTTAAGAAAGCTGGAGCAAAACCCAAATTGAGAGTGAATGGAAAACGTTCCATAAAAAATGCTGTGGTTAATGTGGATTTGAATGTGTCTGCTAGCTATGATGAACATCCCAAAATCAAGCGTAATGGACCTGAGAAAAAAAGGAAACTTGTTGTTGGAGCAACTTTTCGAGTCGGGGATCACGACAGAGTATCAAAATCTGCTTCAGAAGCAATCCTAGCAAATGGAAATGGCAGCATCGGGCATGATCAGAAAGTTCCTAGTTCAGCAGACGTTCCCACCAGACGATGCCCCACGGCACCAGCCTTTGATGCTAGGAAGATGTTGATCGAGAAGGCGAGAACGGAAATCTCGAAGAAATTAGAAGAGATGAAGTTGGCTACAGAAGCTGCTGCTGCAGTAAAGAACACAAAAAAGCATGTTGAAGTTGTAGTCACTGCTGCTTCACTAAAGAAAACAAAAAGGCATGCTGAAGTTGAAGTGGCTGCTGCTTCACTAAAGAAAACAAAAAGACGTGTTGGAGTGGGTCAATCTGGAGGAGCTCCAGGGCATCAATTAGAGACAAAGAAAAGTGAGCCACACTTCCATAGAGATCCTGAGCCCCAAAACATGCCAGTCCCTGACTCTGACTTCCACAATTTTGACGAAGATAGGTCAGAGGAATGTTTTAAGCCAAAGCAAATATGGGCTTTATATGATGAAGATGGCATGCCTCGGTTGTACTGTCTGGTACGTGAAGTGGTCTCAGTCAAACCATTTAAGTTGCTCATTACTTACTTGAGCTCTAAAACTGATAGTGAATTCGGGCCCGTGAACTGGCTGGATAGCGGGTTCACCAAGTCTTGTGGAAATTTCAGAGCTCGACACCTTGATGTTGTTGAGCATGTTAACATATTCTCTCATATTTTGAGCCGCACGAAAGCAGCTAGAGGAGGTATTGTTCAGATATACCCAAAAAGTGGGGATGTCTGGGCTCTGTATCGGAATTGGTCAAAAGATTGGGATAGTTCGACTCCAGATGAAGTGAGGCACCAATATCAAATGGTGGAGGTTCTTGATGATTACTCGGAAGAGATTGGTGTTTGTGTCTCTCCCCTGGTGAAGCTGGCTGGATTTAGGTCAGTATACCAGACTGATACGGATAGAAGCGCCATTCTATGGATTCCAAGAAGAGAGATGTTACGCTTTTCTCACCAGGTGCCCGCATGGTTACTCGACGGAGAAGGGACTAACCTACCTGGTAAGTGTTGGGATCTGGATCCTGCTGCAACTCCAGAAGGACTGCTTAATGCTGCAACTCCAGACGAGCAGCTTCATGCTGCAGCGCCAGACGAGCTACCTCATGCTGCAACAAGAGACGAGCTGCTTCATGCTGCAAAAAGAGACGAGCTGCTTCATGCTGCAACAAGAGACGAGCTGCTTCATGCTGCTGCAACTCCAGATGAGATGATTCATGCTGCTATGGAAGCTGAGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

780

Amino Acids

86.23

Weight (kDa)

8.68

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 66 - 127 5.6e-19 DnaJ domain
Zn_ribbon_20 PF23551 161 - 195 1.2e-13 Zinc beta-ribbon domain
DUF3444 PF11926 489 - 696 1.4e-73 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010314)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G05230 AT2G05250 AT5G35753
fragaria_vesca FvH4_6g40880 FvH4_6g40880
malus_domestica MD09G1123800.v1.1 MD17G1114600.v1.1
prunus_persica Prupe.3G200300_v2.0.a1 Prupe.3G200300_v2.0.a1
pyrus_communis pycom09g04790 pycom17g10590
rosa_chinensis RchiOBHm_Chr2g0156451
rosa_laevigata RLG00000020913
rosa_multiflora Rmu_sc0005392.1_g000017
rosa_roxburghii Rroxscaffold_2G00093030
rosa_rugosa Rorug02G0453200
rosa_samantha Rh2AG519200 Rh2BG531500 Rh2CG504400 Rh2DG540500
rosa_wichuraiana Rw2G042820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 349
Acc65I GGTACC 1 cut(s) 689
AccB1I GGYRCC 5 cut(s) 689, 710, 1162, 1893, 2062
AccB7I CCANNNNNTGG 5 cut(s) 608, 686, 707, 1823, 2022
AccI GTMKAC 2 cut(s) 469, 1989
AccIII TCCGGA 2 cut(s) 115, 525
AciI CCGC 4 cut(s) 165, 1692, 1777, 2067
AclI AACGTT 1 cut(s) 910
AclWI GGATC 5 cut(s) 1050, 1451, 2117, 2124, 2130
AcoI YGGCCR 1 cut(s) 632
AcsI RAATTY 2 cut(s) 1664, 1714
AcuI CTGAAG 3 cut(s) 354, 1053, 1356
AdeI CACNNNGTG 1 cut(s) 2062
AfaI GTAC 4 cut(s) 176, 691, 1586, 1596
AfiI CCNNNNNNNGG 7 cut(s) 608, 647, 686, 707, 1680, 1823, 2022
AflII CTTAAG 1 cut(s) 26
AflIII ACRYGT 1 cut(s) 1378
AgsI TTSAA 9 cut(s) 155, 220, 322, 386, 657, 753, 946, 1289, 1343
AjiI CACGTC 1 cut(s) 1379
AjnI CCWGG 5 cut(s) 715, 1408, 1959, 2058, 2101
AloI GAACNNNNNNTCC 4 cut(s) 1679, 1711, 1788, 1820
Alw21I GWGCWC 4 cut(s) 776, 1408, 1649, 1726
Alw26I GTCTC 7 cut(s) 334, 1418, 1612, 1957, 2214, 2241, 2268
AlwI GGATC 5 cut(s) 1050, 1451, 2117, 2124, 2130
AlwNI CAGNNNCTG 3 cut(s) 183, 1253, 1484
Aor13HI TCCGGA 2 cut(s) 115, 525
AoxI GGCC 4 cut(s) 21, 632, 1670, 2337
ApaI GGGCCC 1 cut(s) 1674
ApoI RAATTY 2 cut(s) 1664, 1714
Asp700I GAANNNNTTC 1 cut(s) 910
Asp718I GGTACC 1 cut(s) 689
AspLEI GCGC 3 cut(s) 317, 2015, 2191
AspS9I GGNCC 7 cut(s) 22, 342, 486, 816, 992, 1670, 1671
AsuHPI GGTGA 4 cut(s) 200, 1690, 1975, 2048
AsuNHI GCTAGC 1 cut(s) 956
AvaII GGWCC 4 cut(s) 342, 486, 816, 992
BaeGI GKGCMC 3 cut(s) 715, 1674, 2067
BaeI ACNNNNGTAYC 2 cut(s) 673, 706
BalI TGGCCA 1 cut(s) 634
BamHI GGATCC 1 cut(s) 2122
BanI GGYRCC 5 cut(s) 689, 710, 1162, 1893, 2062
BanII GRGCYC 7 cut(s) 776, 1408, 1467, 1649, 1674, 1726, 1840
Bbv12I GWGCWC 4 cut(s) 776, 1408, 1649, 1726
BbvCI CCTCAGC 1 cut(s) 2334
BccI CCATC 3 cut(s) 259, 305, 1562
BceAI ACGGC 1 cut(s) 1176
BciT130I CCWGG 5 cut(s) 717, 1410, 1961, 2060, 2103
BclI TGATCA 1 cut(s) 1111
BcoDI GTCTC 7 cut(s) 334, 1418, 1612, 1957, 2214, 2241, 2268
BfaI CTAG 5 cut(s) 957, 1082, 1125, 1179, 1791
BfmI CTRYAG 5 cut(s) 408, 432, 1245, 1260, 2184
BfoI RGCGCY 2 cut(s) 2016, 2192
BfrI CTTAAG 1 cut(s) 26
BglI GCCNNNNNGGC 1 cut(s) 29
Bme1390I CCNGG 5 cut(s) 717, 1410, 1961, 2060, 2103
Bme18I GGWCC 4 cut(s) 342, 486, 816, 992
BmgBI CACGTC 1 cut(s) 1379
BmgT120I GGNCC 7 cut(s) 22, 342, 486, 816, 992, 1670, 1671
BmiI GGNNCC 9 cut(s) 613, 691, 712, 817, 1164, 1672, 1895, 2064, 2124
BmrFI CCNGG 5 cut(s) 717, 1410, 1961, 2060, 2103
BmsI GCATC 5 cut(s) 229, 1110, 1142, 1165, 1423
BmtI GCTAGC 1 cut(s) 960
BpmI CTGGAG 8 cut(s) 816, 897, 1392, 1419, 1863, 2121, 2148, 2286
Bpu10I CCTNAGC 2 cut(s) 1461, 2334
BpuEI CTTGAG 2 cut(s) 214, 1663
BsaAI YACGTR 1 cut(s) 1598
BsaBI GATNNNNATC 3 cut(s) 969, 1190, 2121
BsaI GGTCTC 1 cut(s) 1612
BsaJI CCNNGG 6 cut(s) 629, 715, 1158, 1409, 1577, 1959
BsaWI WCCGGW 2 cut(s) 115, 525
BsaXI ACNNNNNCTCC 2 cut(s) 1788, 1818
Bsc4I CCNNNNNNNGG 7 cut(s) 608, 647, 686, 707, 1680, 1823, 2022
Bse1I ACTGG 3 cut(s) 733, 1478, 1685
Bse3DI GCAATG 1 cut(s) 576
Bse8I GATNNNNATC 3 cut(s) 969, 1190, 2121
BseAI TCCGGA 2 cut(s) 115, 525
BseBI CCWGG 5 cut(s) 717, 1410, 1961, 2060, 2103
BseDI CCNNGG 6 cut(s) 629, 715, 1158, 1409, 1577, 1959
BseGI GGATG 2 cut(s) 970, 1834
BseJI GATNNNNATC 3 cut(s) 969, 1190, 2121
BseLI CCNNNNNNNGG 7 cut(s) 608, 647, 686, 707, 1680, 1823, 2022
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 5 cut(s) 36, 987, 1452, 1623, 2325
BseNI ACTGG 3 cut(s) 733, 1478, 1685
BseRI GAGGAG 3 cut(s) 32, 1416, 1809
BseSI GKGCMC 3 cut(s) 715, 1674, 2067
BseYI CCCAGC 2 cut(s) 601, 700
BshFI GGCC 4 cut(s) 23, 634, 1672, 2339
BshNI GGYRCC 5 cut(s) 689, 710, 1162, 1893, 2062
BsiHKAI GWGCWC 4 cut(s) 776, 1408, 1649, 1726
BsiSI CCGG 2 cut(s) 116, 526
BslFI GGGAC 2 cut(s) 1466, 2104
BslI CCNNNNNNNGG 7 cut(s) 608, 647, 686, 707, 1680, 1823, 2022
BsmAI GTCTC 7 cut(s) 334, 1418, 1612, 1957, 2214, 2241, 2268
BsmBI CGTCTC 3 cut(s) 2214, 2241, 2268
BsmFI GGGAC 2 cut(s) 1466, 2104
BsmI GAATGC 2 cut(s) 317, 652
BsnI GGCC 4 cut(s) 23, 634, 1672, 2339
Bso31I GGTCTC 1 cut(s) 1612
Bsp120I GGGCCC 1 cut(s) 1670
Bsp1286I GDGCHC 9 cut(s) 715, 776, 1408, 1467, 1649, 1674, 1726, 1840, 2067
Bsp13I TCCGGA 2 cut(s) 115, 525
Bsp143I GATC 9 cut(s) 39, 196, 379, 1042, 1111, 1191, 1456, 2116, 2122
BspACI CCGC 4 cut(s) 165, 1692, 1777, 2067
BspANI GGCC 4 cut(s) 23, 634, 1672, 2339
BspCNI CTCAG 5 cut(s) 37, 988, 1453, 1622, 2326
BspEI TCCGGA 2 cut(s) 115, 525
BspLI GGNNCC 9 cut(s) 613, 691, 712, 817, 1164, 1672, 1895, 2064, 2124
BspMAI CTGCAG 3 cut(s) 412, 1264, 2188
BspOI GCTAGC 1 cut(s) 960
BspPI GGATC 5 cut(s) 1050, 1451, 2117, 2124, 2130
BspT107I GGYRCC 5 cut(s) 689, 710, 1162, 1893, 2062
BspTI CTTAAG 1 cut(s) 26
BspTNI GGTCTC 1 cut(s) 1612
BsrDI GCAATG 1 cut(s) 576
BsrI ACTGG 3 cut(s) 733, 1478, 1685
BssECI CCNNGG 6 cut(s) 629, 715, 1158, 1409, 1577, 1959
BssMI GATC 9 cut(s) 39, 196, 379, 1042, 1111, 1191, 1456, 2116, 2122
BssNAI GTATAC 2 cut(s) 470, 1990
BssT1I CCWWGG 1 cut(s) 629
Bst1107I GTATAC 2 cut(s) 470, 1990
Bst2UI CCWGG 5 cut(s) 717, 1410, 1961, 2060, 2103
Bst4CI ACNGT 4 cut(s) 436, 490, 563, 1589
Bst6I CTCTTC 3 cut(s) 1224, 1929, 2029
BstAFI CTTAAG 1 cut(s) 26
BstAPI GCANNNNNTGC 2 cut(s) 501, 710
BstBAI YACGTR 1 cut(s) 1598
BstC8I GCNNGC 5 cut(s) 958, 1332, 1574, 1971, 2067
BstDEI CTNAG 6 cut(s) 45, 81, 996, 1461, 1609, 2334
BstDSI CCRYGG 1 cut(s) 1158
BstEII GGTNACC 1 cut(s) 667
BstF5I GGATG 2 cut(s) 970, 1834
BstH2I RGCGCY 2 cut(s) 2016, 2192
BstHHI GCGC 3 cut(s) 317, 2015, 2191
BstKTI GATC 9 cut(s) 42, 199, 382, 1045, 1114, 1194, 1459, 2119, 2125
BstMAI GTCTC 7 cut(s) 334, 1418, 1612, 1957, 2214, 2241, 2268
BstMBI GATC 9 cut(s) 39, 196, 379, 1042, 1111, 1191, 1456, 2116, 2122
BstNI CCWGG 5 cut(s) 717, 1410, 1961, 2060, 2103
BstNSI RCATGY 6 cut(s) 737, 1286, 1334, 1477, 1576, 1751
BstPI GGTNACC 1 cut(s) 667
BstSCI CCNGG 5 cut(s) 715, 1408, 1959, 2058, 2101
BstSFI CTRYAG 5 cut(s) 408, 432, 1245, 1260, 2184
BstSLI GKGCMC 3 cut(s) 715, 1674, 2067
BstX2I RGATCY 3 cut(s) 1456, 2116, 2122
BstYI RGATCY 3 cut(s) 1456, 2116, 2122
BstZ17I GTATAC 2 cut(s) 470, 1990
BsuRI GGCC 4 cut(s) 23, 634, 1672, 2339
BtgI CCRYGG 1 cut(s) 1158
BtrI CACGTC 1 cut(s) 1379
BtsCI GGATG 2 cut(s) 970, 1834
BtsI GCAGTG 3 cut(s) 99, 249, 1299
BtsIMutI CAGTG 4 cut(s) 99, 249, 773, 1299
Cac8I GCNNGC 5 cut(s) 958, 1332, 1574, 1971, 2067
CaiI CAGNNNCTG 3 cut(s) 183, 1253, 1484
CfoI GCGC 3 cut(s) 317, 2015, 2191
Cfr13I GGNCC 7 cut(s) 22, 342, 486, 816, 992, 1670, 1671
CsiI ACCWGGT 2 cut(s) 2058, 2101
Csp6I GTAC 4 cut(s) 175, 690, 1585, 1595
CspCI CAANNNNNGTGG 2 cut(s) 1690, 1725
CviQI GTAC 4 cut(s) 175, 690, 1585, 1595
DdeI CTNAG 6 cut(s) 45, 81, 996, 1461, 1609, 2334
DpnI GATC 9 cut(s) 41, 198, 381, 1044, 1113, 1193, 1458, 2118, 2124
DpnII GATC 9 cut(s) 39, 196, 379, 1042, 1111, 1191, 1456, 2116, 2122
DraIII CACNNNGTG 1 cut(s) 2062
DrdI GACNNNNNNGTC 1 cut(s) 349
DseDI GACNNNNNNGTC 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 632
Eam1104I CTCTTC 3 cut(s) 1224, 1929, 2029
EarI CTCTTC 3 cut(s) 1224, 1929, 2029
Ecl136II GAGCTC 4 cut(s) 774, 1406, 1647, 1724
Eco130I CCWWGG 1 cut(s) 629
Eco147I AGGCCT 1 cut(s) 2339
Eco24I GRGCYC 7 cut(s) 776, 1408, 1467, 1649, 1674, 1726, 1840
Eco31I GGTCTC 1 cut(s) 1612
Eco47I GGWCC 4 cut(s) 342, 486, 816, 992
Eco53kI GAGCTC 4 cut(s) 774, 1406, 1647, 1724
Eco57I CTGAAG 3 cut(s) 354, 1053, 1356
Eco91I GGTNACC 1 cut(s) 667
EcoICRI GAGCTC 4 cut(s) 774, 1406, 1647, 1724
EcoO109I RGGNCCY 2 cut(s) 22, 816
EcoO65I GGTNACC 1 cut(s) 667
EcoRI GAATTC 1 cut(s) 1664
EcoRII CCWGG 5 cut(s) 715, 1408, 1959, 2058, 2101
EcoT14I CCWWGG 1 cut(s) 629
EcoT22I ATGCAT 1 cut(s) 739
EcoT38I GRGCYC 7 cut(s) 776, 1408, 1467, 1649, 1674, 1726, 1840
ErhI CCWWGG 1 cut(s) 629
Esp3I CGTCTC 3 cut(s) 2214, 2241, 2268
FalI AAGNNNNNCTT 4 cut(s) 356, 388, 2133, 2165
FaqI GGGAC 2 cut(s) 1466, 2104
FauI CCCGC 2 cut(s) 1685, 2074
FauNDI CATATG 1 cut(s) 739
FbaI TGATCA 1 cut(s) 1111
FblI GTMKAC 2 cut(s) 469, 1989
FokI GGATG 2 cut(s) 957, 1841
FriOI GRGCYC 7 cut(s) 776, 1408, 1467, 1649, 1674, 1726, 1840
FspBI CTAG 5 cut(s) 957, 1082, 1125, 1179, 1791
GlaI GCGC 3 cut(s) 316, 2014, 2190
GsaI CCCAGC 2 cut(s) 605, 704
GsuI CTGGAG 8 cut(s) 816, 897, 1392, 1419, 1863, 2121, 2148, 2286
HaeII RGCGCY 2 cut(s) 2016, 2192
HaeIII GGCC 4 cut(s) 23, 634, 1672, 2339
HapII CCGG 2 cut(s) 116, 526
HhaI GCGC 3 cut(s) 317, 2015, 2191
Hin6I GCGC 3 cut(s) 315, 2013, 2189
HinP1I GCGC 3 cut(s) 315, 2013, 2189
HincII GTYRAC 3 cut(s) 10, 538, 1753
HindII GTYRAC 3 cut(s) 10, 538, 1753
HinfI GANTC 8 cut(s) 180, 208, 587, 1035, 1487, 1876, 2026, 2314
HpaI GTTAAC 2 cut(s) 10, 1753
HpaII CCGG 2 cut(s) 116, 526
HphI GGTGA 4 cut(s) 200, 1690, 1975, 2048
Hpy166II GTNNAC 7 cut(s) 10, 470, 538, 1678, 1698, 1753, 1990
Hpy8I GTNNAC 7 cut(s) 10, 470, 538, 1678, 1698, 1753, 1990
Hpy99I CGWCG 1 cut(s) 2084
HpyAV CCTTC 6 cut(s) 28, 91, 625, 1192, 2081, 2135
HpyCH4III ACNGT 4 cut(s) 436, 490, 563, 1589
HpyCH4IV ACGT 4 cut(s) 910, 1137, 1378, 1597
HpyF3I CTNAG 6 cut(s) 45, 81, 996, 1461, 1609, 2334
HpySE526I ACGT 4 cut(s) 910, 1137, 1378, 1597
HspAI GCGC 3 cut(s) 315, 2013, 2189
Kpn2I TCCGGA 2 cut(s) 115, 525
KpnI GGTACC 1 cut(s) 693
Ksp22I TGATCA 1 cut(s) 1111
KspAI GTTAAC 2 cut(s) 10, 1753
Kzo9I GATC 9 cut(s) 39, 196, 379, 1042, 1111, 1191, 1456, 2116, 2122
LmnI GCTCC 8 cut(s) 282, 305, 617, 771, 878, 1022, 1403, 1411
LweI GCATC 5 cut(s) 229, 1110, 1142, 1165, 1423
MabI ACCWGGT 2 cut(s) 2058, 2101
MaeI CTAG 5 cut(s) 957, 1082, 1125, 1179, 1791
MaeII ACGT 4 cut(s) 910, 1137, 1378, 1597
MaeIII GTNAC 5 cut(s) 667, 829, 1297, 2043, 2073
MalI GATC 9 cut(s) 41, 198, 381, 1044, 1113, 1193, 1458, 2118, 2124
MboI GATC 9 cut(s) 39, 196, 379, 1042, 1111, 1191, 1456, 2116, 2122
MflI RGATCY 3 cut(s) 1456, 2116, 2122
MhlI GDGCHC 9 cut(s) 715, 776, 1408, 1467, 1649, 1674, 1726, 1840, 2067
MlsI TGGCCA 1 cut(s) 634
MluNI TGGCCA 1 cut(s) 634
MlyI GAGTC 3 cut(s) 1044, 1481, 1870
MmeI TCCRAC 2 cut(s) 1000, 1363
Mox20I TGGCCA 1 cut(s) 634
Mph1103I ATGCAT 1 cut(s) 739
MroI TCCGGA 2 cut(s) 115, 525
MroXI GAANNNNTTC 1 cut(s) 910
MscI TGGCCA 1 cut(s) 634
MseI TTAA 9 cut(s) 9, 27, 414, 867, 933, 1533, 1623, 1752, 2151
MslI CAYNNNNRTG 5 cut(s) 444, 581, 738, 766, 1734
Msp20I TGGCCA 1 cut(s) 634
MspA1I CMGCKG 1 cut(s) 827
MspCI CTTAAG 1 cut(s) 26
MspI CCGG 2 cut(s) 116, 526
MspR9I CCNGG 5 cut(s) 717, 1410, 1961, 2060, 2103
Mva1269I GAATGC 2 cut(s) 317, 652
MvaI CCWGG 5 cut(s) 717, 1410, 1961, 2060, 2103
NdeI CATATG 1 cut(s) 739
NdeII GATC 9 cut(s) 39, 196, 379, 1042, 1111, 1191, 1456, 2116, 2122
NheI GCTAGC 1 cut(s) 956
NlaIV GGNNCC 9 cut(s) 613, 691, 712, 817, 1164, 1672, 1895, 2064, 2124
NmuCI GTSAC 1 cut(s) 1297
NsiI ATGCAT 1 cut(s) 739
NspI RCATGY 6 cut(s) 737, 1286, 1334, 1477, 1576, 1751
PaeI GCATGC 2 cut(s) 1334, 1576
PceI AGGCCT 1 cut(s) 2339
PcsI WCGNNNNNNNCGW 2 cut(s) 1044, 1214
PctI GAATGC 2 cut(s) 317, 652
PdmI GAANNNNTTC 1 cut(s) 910
PfeI GAWTC 5 cut(s) 180, 208, 587, 2026, 2314
PflMI CCANNNNNTGG 5 cut(s) 608, 686, 707, 1823, 2022
PleI GAGTC 3 cut(s) 1043, 1481, 1870
PpsI GAGTC 3 cut(s) 1043, 1481, 1870
Ppu21I YACGTR 1 cut(s) 1598
PpuMI RGGWCCY 1 cut(s) 816
Psp124BI GAGCTC 4 cut(s) 776, 1408, 1649, 1726
Psp1406I AACGTT 1 cut(s) 910
Psp5II RGGWCCY 1 cut(s) 816
Psp6I CCWGG 5 cut(s) 715, 1408, 1959, 2058, 2101
PspEI GGTNACC 1 cut(s) 667
PspFI CCCAGC 2 cut(s) 601, 700
PspGI CCWGG 5 cut(s) 715, 1408, 1959, 2058, 2101
PspN4I GGNNCC 9 cut(s) 613, 691, 712, 817, 1164, 1672, 1895, 2064, 2124
PspOMI GGGCCC 1 cut(s) 1670
PspPI GGNCC 7 cut(s) 22, 342, 486, 816, 992, 1670, 1671
PspPPI RGGWCCY 1 cut(s) 816
PstI CTGCAG 3 cut(s) 412, 1264, 2188
PstNI CAGNNNCTG 3 cut(s) 183, 1253, 1484
PsuI RGATCY 3 cut(s) 1456, 2116, 2122
PvuII CAGCTG 1 cut(s) 827
RsaI GTAC 4 cut(s) 176, 691, 1586, 1596
RsaNI GTAC 4 cut(s) 175, 690, 1585, 1595
RseI CAYNNNNRTG 5 cut(s) 444, 581, 738, 766, 1734
SacI GAGCTC 4 cut(s) 776, 1408, 1649, 1726
SaqAI TTAA 9 cut(s) 9, 27, 414, 867, 933, 1533, 1623, 1752, 2151
Sau3AI GATC 9 cut(s) 39, 196, 379, 1042, 1111, 1191, 1456, 2116, 2122
Sau96I GGNCC 7 cut(s) 22, 342, 486, 816, 992, 1670, 1671
SchI GAGTC 3 cut(s) 1044, 1481, 1870
ScrFI CCNGG 5 cut(s) 717, 1410, 1961, 2060, 2103
SduI GDGCHC 9 cut(s) 715, 776, 1408, 1467, 1649, 1674, 1726, 1840, 2067
SexAI ACCWGGT 2 cut(s) 2058, 2101
SfaNI GCATC 5 cut(s) 229, 1110, 1142, 1165, 1423
SfcI CTRYAG 5 cut(s) 408, 432, 1245, 1260, 2184
SinI GGWCC 4 cut(s) 342, 486, 816, 992
SmiMI CAYNNNNRTG 5 cut(s) 444, 581, 738, 766, 1734
SmlI CTYRAG 3 cut(s) 26, 229, 1642
SmoI CTYRAG 3 cut(s) 26, 229, 1642
SphI GCATGC 2 cut(s) 1334, 1576
SseBI AGGCCT 1 cut(s) 2339
SsiI CCGC 4 cut(s) 165, 1692, 1777, 2067
SspMI CTAG 5 cut(s) 957, 1082, 1125, 1179, 1791
SstI GAGCTC 4 cut(s) 776, 1408, 1649, 1726
StuI AGGCCT 1 cut(s) 2339
StyD4I CCNGG 5 cut(s) 715, 1408, 1959, 2058, 2101
StyI CCWWGG 1 cut(s) 629
TaaI ACNGT 4 cut(s) 436, 490, 563, 1589
TaiI ACGT 4 cut(s) 913, 1140, 1381, 1600
TaqI TCGA 8 cut(s) 206, 565, 1033, 1194, 1217, 1726, 1874, 2079
TatI WGTACW 2 cut(s) 174, 1584
TauI GCSGC 2 cut(s) 167, 1779
TfiI GAWTC 5 cut(s) 180, 208, 587, 2026, 2314
Tru1I TTAA 9 cut(s) 9, 27, 414, 867, 933, 1533, 1623, 1752, 2151
Tru9I TTAA 9 cut(s) 9, 27, 414, 867, 933, 1533, 1623, 1752, 2151
TscAI CASTG 4 cut(s) 99, 249, 773, 1306
TseFI GTSAC 1 cut(s) 1297
Tsp45I GTSAC 1 cut(s) 1297
TspGWI ACGGA 3 cut(s) 1223, 2018, 2097
TspRI CASTG 4 cut(s) 99, 249, 773, 1306
Van91I CCANNNNNTGG 5 cut(s) 608, 686, 707, 1823, 2022
Vha464I CTTAAG 1 cut(s) 26
VpaK11BI GGWCC 4 cut(s) 342, 486, 816, 992
XapI RAATTY 2 cut(s) 1664, 1714
XceI RCATGY 6 cut(s) 737, 1286, 1334, 1477, 1576, 1751
XcmI CCANNNNNNNNNTGG 1 cut(s) 1545
XmiI GTMKAC 2 cut(s) 469, 1989
XmnI GAANNNNTTC 1 cut(s) 910
XspI CTAG 5 cut(s) 957, 1082, 1125, 1179, 1791
Zsp2I ATGCAT 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.