Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008249

Physical Location & Seq

Genomic Coordinates Reverse (-)

14108214 .. 14110618

Gene Structure 2405 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G35960.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1290 bp

ATGCTAAAATGTGTTTCTGTCCAAATTCAAACCAGTTTTGTCTTGTCTTGTCTTCTTCTTCTTCAGAGCATTGCAATGAAAGTTCCAGATTCTTCTTCACCGACCGGAGTTCTCGAAGAATTCTTCAGAACCGAAGAATTCAATAGCTCCTCCGAGACGGTCAAGAACCCTTCCTCTTCTCGTTTCCGCAAGATGGTTCAGCTTCTCAGAAGCAAATCCAAAAAGTCTCTTGAGAATGTCAAGATTCCGTTTCACAACAATGGTGTTATTAAGAGTTCTTTTAGAAGATGCAGTAGCATGAGAGAAAATCTCAGATTTAGCTCCAATGATTCTCACTTTCTTCTACATTCTCCTAGAAGAATCTTCACCTTCTCTGACCTCAAGTCTGCTACAAACAATTTCTCTTTAGAAAATCTAATTGGGAAAGGAGGTTATGCTGAAGTTTACAAAGGGATGTTACCAAATGGACAAATGGTAGCGATAAAACGGTTGATGCGGGGAAATAGTGAAGAGATCATTGTAGACTTTCTTTCGGAGATGGGTATAATGGCACATGTTAACCATCCCAACATAGCTAAGCTTTTAGGCTATGGAGTTGAAGGAGGAATGCATCTTGTTCTTGAGTTATCACCTCATGGCAGCTTAGCTTCTATGCTCTATAGCTCCAAGGAGAAGATGAAATGGAGCATTAGGTACAAGATAGCATTAGGAGTTGCGGAGGGTCTAGTGTATCTTCATAGAGGATGTCATAGGAGAATTATACATAGAGATATCAAAGCCGCAAACATTCTTCTCACACATGATTTTTCTCCTCAGATATGTGACTTTGGGCTTGCGAAGTGGTTACCTGAAAATTGGACACACCACATTGTTTCCAAATTTGAGGGCACATTCGGGTATCTTGCTCCAGAGTACTTAACACATGGGATAGTAGATGAAAAGACCGATGTATTCGCCTTGGGTGTACTTTTACTGGAACTTGTTACCGGACGACGAGCTCTCGATTACTCCAAGCAAAGTCTTGTTCTATGGGCTAAACCATTGATGAAGAAGAACAAAATTAGAGAGCTAATCGATCCATCTCTGGCGGGTGAGTACGAATGGAGGCAGATAAAACTTGTGCTTTTGGCTGCTGCGTTATCAATCCAACAATCATCGATAGAGAGGCCTGAAATGAGTCAGGTTGTTGAGATCCTCAAAGGCAACTTGAAAGATCTCAAGTGCATCATGAAATGCAGAGTTCCCTTTTATAGGAAAGCTTTCAGGGATGAAGTTGGCAAAAAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

429

Amino Acids

48.68

Weight (kDa)

9.52

Isoelectric Point (pI)

46.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	135 - 340	1.8e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	136 - 335	9.7e-42	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35960 AT5G35960
fragaria_vesca	FvH4_2g24300
malus_domestica	MD03G1272700.v1.1 MD11G1293600.v1.1
prunus_persica	Prupe.8G249200_v2.0.a1
pyrus_communis	pycom03g21720 pycom11g25980
rosa_chinensis	RchiOBHm_Chr6g0292171
rosa_laevigata	RLG00000012056
rosa_multiflora	Rmu_sc0000502.1_g000005 Rmu_sc0006021.1_g000005
rosa_roxburghii	Rroxscaffold_7G00175390
rosa_rugosa	Rorug06G0017700 Rorug06G0017800 Rorug06G0017900 Rorug06G0223900 Rorug06G0224000 Rorug06G0224100 Rorug06G0224200
rosa_samantha	Rh6AG337300 Rh6DG337500
rosa_wichuraiana	Rw6G029360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	522
AciI	CCGC	5 cut(s)	187, 496, 716, 780, 1088
AclWI	GGATC	2 cut(s)	1070, 1186
AcsI	RAATTY	4 cut(s)	24, 119, 137, 878
AcuI	CTGAAG	3 cut(s)	47, 109, 459
AfaI	GTAC	4 cut(s)	695, 914, 966, 1097
AfiI	CCNNNNNNNGG	2 cut(s)	193, 1251
AflIII	ACRYGT	1 cut(s)	553
AgsI	TTSAA	4 cut(s)	29, 142, 599, 1210
AhdI	GACNNNNNGTC	1 cut(s)	382
AjuI	GAANNNNNNNTTGG	2 cut(s)	402, 434
Alw21I	GWGCWC	1 cut(s)	1000
Alw26I	GTCTC	2 cut(s)	149, 231
AlwI	GGATC	2 cut(s)	1070, 1186
AoxI	GGCC	1 cut(s)	1166
ApeKI	GCWGC	3 cut(s)	639, 1130, 1133
ApoI	RAATTY	4 cut(s)	24, 119, 137, 878
Asp700I	GAANNNNTTC	1 cut(s)	1259
AsuHPI	GGTGA	4 cut(s)	90, 358, 621, 1103
BaeGI	GKGCMC	1 cut(s)	890
BanII	GRGCYC	1 cut(s)	1000
BbsI	GAAGAC	1 cut(s)	44
Bbv12I	GWGCWC	1 cut(s)	1000
BbvI	GCAGC	3 cut(s)	651, 1117, 1120
BccI	CCATC	4 cut(s)	187, 532, 570, 1087
BcoDI	GTCTC	2 cut(s)	149, 231
BfaI	CTAG	2 cut(s)	354, 725
BfmI	CTRYAG	1 cut(s)	658
BglII	AGATCT	1 cut(s)	1213
BisI	GCNGC	4 cut(s)	640, 780, 1131, 1134
BlpI	GCTNAGC	2 cut(s)	576, 643
BlsI	GCNGC	4 cut(s)	641, 781, 1132, 1135
BmcAI	AGTACT	1 cut(s)	914
BmeRI	GACNNNNNGTC	1 cut(s)	382
BmsI	GCATC	4 cut(s)	278, 483, 619, 1233
BpiI	GAAGAC	1 cut(s)	44
BplI	GAGNNNNNCTC	2 cut(s)	294, 326
BpmI	CTGGAG	1 cut(s)	891
Bpu1102I	GCTNAGC	2 cut(s)	576, 643
BpuEI	CTTGAG	4 cut(s)	251, 365, 641, 1202
Bsa29I	ATCGAT	2 cut(s)	1074, 1157
BsaJI	CCNNGG	2 cut(s)	666, 957
BsaWI	WCCGGW	2 cut(s)	104, 986
Bsc4I	CCNNNNNNNGG	2 cut(s)	193, 1251
Bse1I	ACTGG	2 cut(s)	33, 978
Bse3DI	GCAATG	2 cut(s)	69, 81
BseCI	ATCGAT	2 cut(s)	1074, 1157
BseDI	CCNNGG	2 cut(s)	666, 957
BseGI	GGATG	4 cut(s)	459, 562, 749, 1273
BseLI	CCNNNNNNNGG	2 cut(s)	193, 1251
BseMI	GCAATG	2 cut(s)	69, 81
BseMII	CTCAG	3 cut(s)	220, 325, 827
BseNI	ACTGG	2 cut(s)	33, 978
BseRI	GAGGAG	2 cut(s)	139, 801
BseSI	GKGCMC	1 cut(s)	890
BseXI	GCAGC	3 cut(s)	651, 1117, 1120
Bsh1285I	CGRYCG	1 cut(s)	105
BshFI	GGCC	1 cut(s)	1168
BshVI	ATCGAT	2 cut(s)	1074, 1157
BsiEI	CGRYCG	1 cut(s)	105
BsiHKAI	GWGCWC	1 cut(s)	1000
BsiSI	CCGG	2 cut(s)	105, 987
BslI	CCNNNNNNNGG	2 cut(s)	193, 1251
BsmAI	GTCTC	2 cut(s)	149, 231
BsmBI	CGTCTC	1 cut(s)	149
BsmI	GAATGC	1 cut(s)	612
BsnI	GGCC	1 cut(s)	1168
Bsp1286I	GDGCHC	2 cut(s)	890, 1000
Bsp143I	GATC	4 cut(s)	513, 1075, 1191, 1213
Bsp1720I	GCTNAGC	2 cut(s)	576, 643
BspACI	CCGC	5 cut(s)	187, 496, 716, 780, 1088
BspANI	GGCC	1 cut(s)	1168
BspCNI	CTCAG	3 cut(s)	219, 324, 826
BspDI	ATCGAT	2 cut(s)	1074, 1157
BspHI	TCATGA	1 cut(s)	1227
BspPI	GGATC	2 cut(s)	1070, 1186
BsrDI	GCAATG	2 cut(s)	69, 81
BsrI	ACTGG	2 cut(s)	33, 978
BssECI	CCNNGG	2 cut(s)	666, 957
BssMI	GATC	4 cut(s)	513, 1075, 1191, 1213
BssT1I	CCWWGG	2 cut(s)	666, 957
Bst4CI	ACNGT	2 cut(s)	160, 489
Bst6I	CTCTTC	2 cut(s)	181, 504
BstC8I	GCNNGC	1 cut(s)	834
BstDEI	CTNAG	5 cut(s)	206, 311, 576, 643, 813
BstEII	GGTNACC	1 cut(s)	843
BstENI	CCTNNNNNAGG	1 cut(s)	1249
BstF5I	GGATG	4 cut(s)	459, 562, 749, 1273
BstKTI	GATC	4 cut(s)	516, 1078, 1194, 1216
BstMAI	GTCTC	2 cut(s)	149, 231
BstMBI	GATC	4 cut(s)	513, 1075, 1191, 1213
BstMCI	CGRYCG	1 cut(s)	105
BstNSI	RCATGY	1 cut(s)	557
BstPI	GGTNACC	1 cut(s)	843
BstSFI	CTRYAG	1 cut(s)	658
BstSLI	GKGCMC	1 cut(s)	890
BstV1I	GCAGC	3 cut(s)	651, 1117, 1120
BstV2I	GAAGAC	1 cut(s)	44
BstX2I	RGATCY	2 cut(s)	1191, 1213
BstYI	RGATCY	2 cut(s)	1191, 1213
Bsu15I	ATCGAT	2 cut(s)	1074, 1157
BsuRI	GGCC	1 cut(s)	1168
BsuTUI	ATCGAT	2 cut(s)	1074, 1157
BtsCI	GGATG	4 cut(s)	459, 562, 749, 1273
Cac8I	GCNNGC	1 cut(s)	834
CciI	TCATGA	1 cut(s)	1227
ClaI	ATCGAT	2 cut(s)	1074, 1157
Csp6I	GTAC	4 cut(s)	694, 913, 965, 1096
CviAII	CATG	6 cut(s)	298, 554, 635, 800, 923, 1228
CviQI	GTAC	4 cut(s)	694, 913, 965, 1096
DdeI	CTNAG	5 cut(s)	206, 311, 576, 643, 813
DpnI	GATC	4 cut(s)	515, 1077, 1193, 1215
DpnII	GATC	4 cut(s)	513, 1075, 1191, 1213
DriI	GACNNNNNGTC	1 cut(s)	382
Eam1104I	CTCTTC	2 cut(s)	181, 504
Eam1105I	GACNNNNNGTC	1 cut(s)	382
EarI	CTCTTC	2 cut(s)	181, 504
Ecl136II	GAGCTC	1 cut(s)	998
Eco130I	CCWWGG	2 cut(s)	666, 957
Eco147I	AGGCCT	1 cut(s)	1168
Eco24I	GRGCYC	1 cut(s)	1000
Eco32I	GATATC	1 cut(s)	772
Eco53kI	GAGCTC	1 cut(s)	998
Eco57I	CTGAAG	3 cut(s)	47, 109, 459
Eco91I	GGTNACC	1 cut(s)	843
EcoICRI	GAGCTC	1 cut(s)	998
EcoNI	CCTNNNNNAGG	1 cut(s)	1249
EcoO65I	GGTNACC	1 cut(s)	843
EcoRI	GAATTC	2 cut(s)	119, 137
EcoRV	GATATC	1 cut(s)	772
EcoT14I	CCWWGG	2 cut(s)	666, 957
EcoT22I	ATGCAT	1 cut(s)	612
EcoT38I	GRGCYC	1 cut(s)	1000
ErhI	CCWWGG	2 cut(s)	666, 957
Esp3I	CGTCTC	1 cut(s)	149
FaeI	CATG	6 cut(s)	301, 557, 638, 803, 926, 1231
FatI	CATG	6 cut(s)	297, 553, 634, 799, 922, 1227
FauI	CCCGC	2 cut(s)	489, 1081
FblI	GTMKAC	1 cut(s)	522
Fnu4HI	GCNGC	4 cut(s)	640, 780, 1131, 1134
FokI	GGATG	4 cut(s)	466, 549, 756, 1280
FriOI	GRGCYC	1 cut(s)	1000
Fsp4HI	GCNGC	4 cut(s)	640, 780, 1131, 1134
FspBI	CTAG	2 cut(s)	354, 725
GluI	GCNGC	4 cut(s)	640, 780, 1131, 1134
GsuI	CTGGAG	1 cut(s)	891
HaeIII	GGCC	1 cut(s)	1168
HapII	CCGG	2 cut(s)	105, 987
Hin1II	CATG	6 cut(s)	301, 557, 638, 803, 926, 1231
HincII	GTYRAC	1 cut(s)	559
HindII	GTYRAC	1 cut(s)	559
HindIII	AAGCTT	2 cut(s)	578, 1257
HinfI	GANTC	5 cut(s)	89, 244, 329, 360, 1177
HpaI	GTTAAC	1 cut(s)	559
HpaII	CCGG	2 cut(s)	105, 987
HphI	GGTGA	4 cut(s)	90, 358, 621, 1103
Hpy166II	GTNNAC	4 cut(s)	445, 523, 559, 965
Hpy188I	TCNGA	8 cut(s)	66, 128, 154, 209, 314, 376, 535, 816
Hpy188III	TCNNGA	9 cut(s)	86, 113, 163, 230, 241, 620, 908, 1001, 1228
Hpy8I	GTNNAC	4 cut(s)	445, 523, 559, 965
Hpy99I	CGWCG	1 cut(s)	996
HpyAV	CCTTC	3 cut(s)	180, 379, 593
HpyCH4III	ACNGT	2 cut(s)	160, 489
HpyCH4V	TGCA	5 cut(s)	74, 291, 610, 1224, 1236
HpyF3I	CTNAG	5 cut(s)	206, 311, 576, 643, 813
Hsp92II	CATG	6 cut(s)	301, 557, 638, 803, 926, 1231
KspAI	GTTAAC	1 cut(s)	559
Kzo9I	GATC	4 cut(s)	513, 1075, 1191, 1213
LmnI	GCTCC	5 cut(s)	152, 326, 668, 684, 910
Lsp1109I	GCAGC	3 cut(s)	651, 1117, 1120
LweI	GCATC	4 cut(s)	278, 483, 619, 1233
MaeI	CTAG	2 cut(s)	354, 725
MaeIII	GTNAC	4 cut(s)	456, 821, 843, 982
MalI	GATC	4 cut(s)	515, 1077, 1193, 1215
MboI	GATC	4 cut(s)	513, 1075, 1191, 1213
MflI	RGATCY	2 cut(s)	1191, 1213
MhlI	GDGCHC	2 cut(s)	890, 1000
MluCI	AATT	9 cut(s)	24, 119, 137, 397, 417, 756, 853, 878, 1059
MlyI	GAGTC	1 cut(s)	1186
MmeI	TCCRAC	1 cut(s)	1171
Mph1103I	ATGCAT	1 cut(s)	612
MroXI	GAANNNNTTC	1 cut(s)	1259
MseI	TTAA	3 cut(s)	270, 558, 917
MslI	CAYNNNNRTG	2 cut(s)	74, 258
MspI	CCGG	2 cut(s)	105, 987
Mva1269I	GAATGC	1 cut(s)	612
NdeII	GATC	4 cut(s)	513, 1075, 1191, 1213
NlaIII	CATG	6 cut(s)	301, 557, 638, 803, 926, 1231
NmuCI	GTSAC	1 cut(s)	821
NsiI	ATGCAT	1 cut(s)	612
NspI	RCATGY	1 cut(s)	557
PagI	TCATGA	1 cut(s)	1227
PceI	AGGCCT	1 cut(s)	1168
PciI	ACATGT	1 cut(s)	553
PctI	GAATGC	1 cut(s)	612
PdmI	GAANNNNTTC	1 cut(s)	1259
PfeI	GAWTC	4 cut(s)	89, 244, 329, 360
PkrI	GCNGC	4 cut(s)	641, 781, 1132, 1135
PleI	GAGTC	1 cut(s)	1185
PpsI	GAGTC	1 cut(s)	1185
PscI	ACATGT	1 cut(s)	553
Psp124BI	GAGCTC	1 cut(s)	1000
PspEI	GGTNACC	1 cut(s)	843
PsuI	RGATCY	2 cut(s)	1191, 1213
RsaI	GTAC	4 cut(s)	695, 914, 966, 1097
RsaNI	GTAC	4 cut(s)	694, 913, 965, 1096
RseI	CAYNNNNRTG	2 cut(s)	74, 258
SacI	GAGCTC	1 cut(s)	1000
SaqAI	TTAA	3 cut(s)	270, 558, 917
SatI	GCNGC	4 cut(s)	640, 780, 1131, 1134
Sau3AI	GATC	4 cut(s)	513, 1075, 1191, 1213
ScaI	AGTACT	1 cut(s)	914
SchI	GAGTC	1 cut(s)	1186
SduI	GDGCHC	2 cut(s)	890, 1000
SfaNI	GCATC	4 cut(s)	278, 483, 619, 1233
SfcI	CTRYAG	1 cut(s)	658
SmiMI	CAYNNNNRTG	2 cut(s)	74, 258
SmlI	CTYRAG	4 cut(s)	230, 380, 620, 1217
SmoI	CTYRAG	4 cut(s)	230, 380, 620, 1217
Sse9I	AATT	9 cut(s)	24, 119, 137, 397, 417, 756, 853, 878, 1059
SseBI	AGGCCT	1 cut(s)	1168
SsiI	CCGC	5 cut(s)	187, 496, 716, 780, 1088
SspMI	CTAG	2 cut(s)	354, 725
SstI	GAGCTC	1 cut(s)	1000
StuI	AGGCCT	1 cut(s)	1168
StyI	CCWWGG	2 cut(s)	666, 957
TaaI	ACNGT	2 cut(s)	160, 489
TaqI	TCGA	4 cut(s)	114, 1002, 1074, 1157
TaqII	GACCGA	1 cut(s)	959
TasI	AATT	9 cut(s)	24, 119, 137, 397, 417, 756, 853, 878, 1059
TatI	WGTACW	2 cut(s)	912, 964
TauI	GCSGC	1 cut(s)	782
TfiI	GAWTC	4 cut(s)	89, 244, 329, 360
Tru1I	TTAA	3 cut(s)	270, 558, 917
Tru9I	TTAA	3 cut(s)	270, 558, 917
TseFI	GTSAC	1 cut(s)	821
TseI	GCWGC	3 cut(s)	639, 1130, 1133
Tsp45I	GTSAC	1 cut(s)	821
TspDTI	ATGAA	7 cut(s)	92, 692, 725, 951, 1061, 1244, 1284
TspGWI	ACGGA	1 cut(s)	237
XagI	CCTNNNNNAGG	1 cut(s)	1249
XapI	RAATTY	4 cut(s)	24, 119, 137, 878
XceI	RCATGY	1 cut(s)	557
XmiI	GTMKAC	1 cut(s)	522
XmnI	GAANNNNTTC	1 cut(s)	1259
XspI	CTAG	2 cut(s)	354, 725
ZrmI	AGTACT	1 cut(s)	914
Zsp2I	ATGCAT	1 cut(s)	612

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.