Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0008249

Physical Location & Seq

Genomic Coordinates Forward (+)

2393740 .. 2396294

Gene Structure 2555 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0017900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2466 bp

ATGGTTGATCACTGTATTGGTATGGTTTCGGAATGTTTTAACTTATTGGACCGAAAGGCTTATGGTGGAAATGTGGGAATCAAAGTGGATATAGCAAAGGCATTTGACACTCTGAATTGGGATTTTCTGCTCCGAGTTTTAAGCAATTTTGGGTTCTCTCCTACTTTCACTAGTTGGGTCAGCTCTATTCTTTACTCAGCTAGATTATCTCTTTTAATCAATGGCTCTCCTCAAGGTTTTTTTAAGTGTGCTCGTGGTGTTAGACAAGGAGATCCTCTTTCTCCACTCTTATTCTGCCTTGCAGAGGAGGCTCTCAGCCGGGGTCTTCACCTACTTCTCCAAACCCGTCAATTGAAGCCAATTTCTTTTCCCAGAAATTGCACTGCACCGTCTCATGTGTTATATGCAGATGACCTCATGGTGTTTTGCCGTGGGGACAAAAACTCTCTTAAACGTCTGCGTGGGTTTTTTGACCGTTATAGTGCAGCTTCAGGGCAATTCATTAATGCGGAGAAAAGTACTTTCTATCTTGGAGTTAGGTCTCGTCATAGGAAAGGTACAGTTCAACGTATTTTGGGTTTTCATGCAGGTCGTCTACCTTTTATTTATCTAGGAGTTCCTATATTTTGTGGAAAACCCCGCAGCTGTCATCTCCAAGTTATTGCCGATAGAGCTAAGTCAAGGTTAATGGGTTGGCAGGGGAGGCTTCTCTCTATGGCTGGGCGTACTCAACTTGTGCAATCTGTTTTTCAGAGTATGCTTCTTCATAGTTTTTCAGTGTATCAATGGCCTGCTTACCTTGTGAAGAAGTTATCTACTTGGGCGCGTAATTTTATATGGTCCGGAAATATTGAGACTCGGAAAATTGTCACTGTTGCTTGGACTCAAGTTTGTGCTCCGAAAAAAGAGGGCGGGGTTGGTATTCGTGACCTGGCAACTCTTAATTCTGCGGCTGTTCTAAGGTTTGCATGGAACTCTTTCACTTCTTTGAATCAATGGGGTGATTATATGCGAACTCGTTACCCTATTGTATCTAGTTCCAAAGTGAGTTATCGCAAATCATCAATCTGGCCAGGCTTCCGTTCTATTGCTCTTCATATCACCCACAATTGTCGTTGGATTATTGGAGATGGTCGTTCAGTGTCCTTTTGGAAAGATAAATGGCTTCAGGATCCAATTTTGAAAATTTTGGGTTTTTTCGACTGGTCAGGATTCAGTGATTTACGAGTGGCAGATTTCATCTCAGATCAGTCTTGGCAGTTCCCCTCTTTTTTCCTAGATACCTTTCCAGACTTGTACAGAAAAATTTCTGATATTTGTCTCCCTTTAGACACAGAACCTGATATGCTAATTTGGGAGTCTACAGCCTCTGGAGAGTTATCTTTCACTAACTCTTATAACTTGCTTAGACGTCACTTTATTTTGAGAGACTGGGCTTCTACTATTTGGCATTCCTTCATTCCTCCGCGCTATTCTTTCTTGGCTTGGAGGATTCTCCTTGATCGCTTGCCCACTGATGATCGGTTGAAAAGAGGTGGGATTCCGGTTGTGTCCATCTGCCAATTATGCAACAGTTCGGCAGAGTCAGCGTTGCACCTATTTTTACATTGTCCTTTTTCCCAACATCTTTGGGGATGGCTGGCAACTCAGTTTGGAACCTCACTTCCTGCTTATAGCTCTCTGTTGGATTTTTGGGTTGGCTTTTGTCACAAGGTTTTCTCCCCTCAGCTTTATAATTTGTGGTTGGCAGCTGGCTTGCTTACTTTCATGGAAATTTGGAAGGCGCGAAATAGACTCAGGTTTGATGATCGATCTCCTATCTTTTCTACACTGTGTTGTTCAATTATGGCATGGCTTCGGCAATTTGGTTCCCTTGTTCCTGGTCACTTTAAGGGCGTCCTTGATTCCCGCCTATTATCCTCCCTTGGGGTGTGCCCCAAGCCCCGTAATGCGCCCAAGATTCAACGTGTCATATGGCATCCCCCCCTCTTTCCTTGGGTCAAAGTTAACACTGATGGTCTGGCGAAAGGTAATCCTGGTCCTGCAGCTTGTGGGGGAGTCTTTCGTGATGCCTCTGGGGTTTATTTAGGGAGTTTTTGTCAACCTCTTGGTCGCAATTCATCCTTCTATGCTGAACTTTGTGCGGTTATTGTGTCTATTGAAGTTGCTTTCACCAGAGGTTGGACTACTCTGTGGTTGGAAAGCGATTCTACTAGTGTTTTAGCATCTCTTTCCTCCATTTCTTTCTCTCCTCCATGGGACTTGAGAGTTAGATGGCAAAACTGCCTGAAGAACATTCAACAAATGCAATTTCGGAGCACTCATATTTTTAGGGAAGGGAATGCAGCTGCTGATAAGATGGCAAACTTAGGTGTTTCAAAGCACTCATTTACTTGGTATCCTAGCCCTCCTGCAGAGCTTCACAGAGGCTTCCTTCCCAGGAACCCCAGGAATTTGTGCGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

821

Amino Acids

93.29

Weight (kDa)

9.73

Isoelectric Point (pI)

43.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RVT_1	PF00078	22 - 207	3e-22	Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT	PF13966	462 - 544	1.3e-23	zinc-binding in reverse transcriptase
RVT_3	PF13456	670 - 790	2.2e-16	Reverse transcriptase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35960 AT5G35960
fragaria_vesca	FvH4_2g24300
malus_domestica	MD03G1272700.v1.1 MD11G1293600.v1.1
prunus_persica	Prupe.8G249200_v2.0.a1
pyrus_communis	pycom03g21720 pycom11g25980
rosa_chinensis	RchiOBHm_Chr6g0292171
rosa_laevigata	RLG00000012056
rosa_multiflora	Rmu_sc0000502.1_g000005 Rmu_sc0006021.1_g000005
rosa_roxburghii	Rroxscaffold_7G00175390
rosa_rugosa	Rorug06G0017700 Rorug06G0017800 Rorug06G0017900 Rorug06G0223900 Rorug06G0224000 Rorug06G0224100 Rorug06G0224200
rosa_samantha	Rh6AG337300 Rh6DG337500
rosa_wichuraiana	Rw6G029360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1398, 1734
AatII	GACGTC	1 cut(s)	1414
Acc16I	TGCGCA	1 cut(s)	2461
Acc36I	ACCTGC	1 cut(s)	578
AccB7I	CCANNNNNTGG	1 cut(s)	2181
AccI	GTMKAC	2 cut(s)	595, 1361
AccII	CGCG	3 cut(s)	826, 1468, 1786
AccIII	TCCGGA	1 cut(s)	842
AciI	CCGC	7 cut(s)	509, 640, 912, 950, 1466, 1909, 2144
AclWI	GGATC	3 cut(s)	266, 1166, 1179
AcoI	YGGCCR	1 cut(s)	1070
AcsI	RAATTY	4 cut(s)	1185, 1305, 1773, 2452
AcuI	CTGAAG	3 cut(s)	474, 1151, 2309
AcyI	GRCGYC	2 cut(s)	1411, 1896
AfaI	GTAC	4 cut(s)	520, 559, 727, 1298
AfiI	CCNNNNNNNGG	3 cut(s)	304, 1926, 2181
AflIII	ACRYGT	1 cut(s)	1966
AhlI	ACTAGT	2 cut(s)	170, 2213
AjnI	CCWGG	6 cut(s)	930, 1072, 1879, 2035, 2438, 2447
AloI	GAACNNNNNNTCC	2 cut(s)	1648, 1680
Alw21I	GWGCWC	3 cut(s)	253, 898, 2321
Alw26I	GTCTC	5 cut(s)	396, 546, 848, 1325, 1422
AlwI	GGATC	3 cut(s)	266, 1166, 1179
AlwNI	CAGNNNCTG	3 cut(s)	1340, 1370, 2351
Aor13HI	TCCGGA	1 cut(s)	842
AoxI	GGCC	2 cut(s)	788, 1070
ApeKI	GCWGC	6 cut(s)	485, 642, 1748, 2045, 2345, 2348
ApoI	RAATTY	4 cut(s)	1185, 1305, 1773, 2452
AseI	ATTAAT	1 cut(s)	504
Asp700I	GAANNNNTTC	2 cut(s)	977, 1306
AspLEI	GCGC	5 cut(s)	826, 1470, 1786, 1954, 2462
AspS9I	GGNCC	3 cut(s)	49, 840, 2039
AsuC2I	CCSGG	1 cut(s)	320
AsuHPI	GGTGA	4 cut(s)	320, 1013, 1093, 2164
AvaII	GGWCC	3 cut(s)	49, 840, 2039
BaeGI	GKGCMC	1 cut(s)	1937
BalI	TGGCCA	1 cut(s)	1072
BamHI	GGATCC	1 cut(s)	1171
BauI	CACGAG	1 cut(s)	252
BbsI	GAAGAC	1 cut(s)	317
Bbv12I	GWGCWC	3 cut(s)	253, 898, 2321
BbvCI	CCTCAGC	1 cut(s)	1725
BbvI	GCAGC	6 cut(s)	497, 654, 1760, 2057, 2335, 2357
BccI	CCATC	6 cut(s)	1124, 1562, 1629, 2009, 2268, 2353
BceAI	ACGGC	1 cut(s)	414
BciT130I	CCWGG	6 cut(s)	932, 1074, 1881, 2037, 2440, 2449
BciVI	GTATCC	1 cut(s)	2409
BclI	TGATCA	1 cut(s)	7
BcnI	CCSGG	1 cut(s)	320
BcoDI	GTCTC	5 cut(s)	396, 546, 848, 1325, 1422
BcuI	ACTAGT	2 cut(s)	170, 2213
BfaI	CTAG	7 cut(s)	171, 201, 611, 1035, 1277, 2214, 2403
BfmI	CTRYAG	3 cut(s)	1362, 2043, 2412
BfuAI	ACCTGC	1 cut(s)	578
BfuI	GTATCC	1 cut(s)	2409
BisI	GCNGC	7 cut(s)	486, 643, 951, 1749, 2046, 2346, 2349
BlsI	GCNGC	7 cut(s)	487, 644, 952, 1750, 2047, 2347, 2350
BmcAI	AGTACT	1 cut(s)	520
Bme1390I	CCNGG	7 cut(s)	320, 932, 1074, 1881, 2037, 2440, 2449
Bme18I	GGWCC	3 cut(s)	49, 840, 2039
BmgT120I	GGNCC	3 cut(s)	49, 840, 2039
BmiI	GGNNCC	4 cut(s)	1173, 1657, 1870, 2444
BmrFI	CCNGG	7 cut(s)	320, 932, 1074, 1881, 2037, 2440, 2449
BmrI	ACTGGG	1 cut(s)	1441
BmsI	GCATC	3 cut(s)	1987, 2059, 2234
BmuI	ACTGGG	1 cut(s)	1441
BpiI	GAAGAC	1 cut(s)	317
BpmI	CTGGAG	1 cut(s)	1392
Bpu10I	CCTNAGC	1 cut(s)	1725
BpuEI	CTTGAG	3 cut(s)	216, 870, 2284
BpuMI	CCSGG	1 cut(s)	320
Bsa29I	ATCGAT	1 cut(s)	1810
BsaHI	GRCGYC	2 cut(s)	1411, 1896
BsaI	GGTCTC	1 cut(s)	546
BsaJI	CCNNGG	7 cut(s)	319, 430, 1924, 1994, 2255, 2438, 2447
BsaWI	WCCGGW	2 cut(s)	842, 1543
BsaXI	ACNNNNNCTCC	2 cut(s)	2083, 2113
Bsc4I	CCNNNNNNNGG	3 cut(s)	304, 1926, 2181
Bse1I	ACTGG	2 cut(s)	1208, 1436
BseAI	TCCGGA	1 cut(s)	842
BseBI	CCWGG	6 cut(s)	932, 1074, 1881, 2037, 2440, 2449
BseCI	ATCGAT	1 cut(s)	1810
BseDI	CCNNGG	7 cut(s)	319, 430, 1924, 1994, 2255, 2438, 2447
BseGI	GGATG	3 cut(s)	1640, 1978, 2120
BseLI	CCNNNNNNNGG	3 cut(s)	304, 1926, 2181
BseMII	CTCAG	6 cut(s)	210, 328, 1257, 1661, 1739, 1810
BseNI	ACTGG	2 cut(s)	1208, 1436
BseRI	GAGGAG	3 cut(s)	219, 320, 2241
BseSI	GKGCMC	1 cut(s)	1937
BseXI	GCAGC	6 cut(s)	497, 654, 1760, 2057, 2335, 2357
BseYI	CCCAGC	1 cut(s)	719
BsgI	GTGCAG	2 cut(s)	369, 504
Bsh1236I	CGCG	3 cut(s)	826, 1468, 1786
BshFI	GGCC	2 cut(s)	790, 1072
BshVI	ATCGAT	1 cut(s)	1810
BsiHKAI	GWGCWC	3 cut(s)	253, 898, 2321
BsiSI	CCGG	3 cut(s)	319, 843, 1544
BslFI	GGGAC	2 cut(s)	449, 2273
BslI	CCNNNNNNNGG	3 cut(s)	304, 1926, 2181
BsmAI	GTCTC	5 cut(s)	396, 546, 848, 1325, 1422
BsmBI	CGTCTC	1 cut(s)	396
BsmFI	GGGAC	2 cut(s)	449, 2273
BsmI	GAATGC	2 cut(s)	1450, 2347
BsnI	GGCC	2 cut(s)	790, 1072
Bso31I	GGTCTC	1 cut(s)	546
Bsp1286I	GDGCHC	4 cut(s)	253, 898, 1937, 2321
Bsp13I	TCCGGA	1 cut(s)	842
Bsp1407I	TGTACA	1 cut(s)	1296
Bsp143I	GATC	8 cut(s)	7, 271, 1171, 1246, 1501, 1519, 1807, 1811
Bsp19I	CCATGG	1 cut(s)	2255
BspACI	CCGC	7 cut(s)	509, 640, 912, 950, 1466, 1909, 2144
BspANI	GGCC	2 cut(s)	790, 1072
BspCNI	CTCAG	6 cut(s)	209, 327, 1256, 1660, 1738, 1809
BspDI	ATCGAT	1 cut(s)	1810
BspEI	TCCGGA	1 cut(s)	842
BspFNI	CGCG	3 cut(s)	826, 1468, 1786
BspLI	GGNNCC	4 cut(s)	1173, 1657, 1870, 2444
BspMAI	CTGCAG	2 cut(s)	2047, 2416
BspMI	ACCTGC	1 cut(s)	578
BspPI	GGATC	3 cut(s)	266, 1166, 1179
BspQI	GCTCTTC	1 cut(s)	1098
BspTNI	GGTCTC	1 cut(s)	546
BsrGI	TGTACA	1 cut(s)	1296
BsrI	ACTGG	2 cut(s)	1208, 1436
BssECI	CCNNGG	7 cut(s)	319, 430, 1924, 1994, 2255, 2438, 2447
BssMI	GATC	8 cut(s)	7, 271, 1171, 1246, 1501, 1519, 1807, 1811
BssNI	GRCGYC	2 cut(s)	1411, 1896
BssSI	CACGAG	1 cut(s)	252
BssT1I	CCWWGG	3 cut(s)	1924, 1994, 2255
Bst2BI	CACGAG	1 cut(s)	252
Bst2UI	CCWGG	6 cut(s)	932, 1074, 1881, 2037, 2440, 2449
Bst4CI	ACNGT	7 cut(s)	14, 390, 476, 562, 874, 1574, 1833
Bst6I	CTCTTC	1 cut(s)	1098
BstACI	GRCGYC	2 cut(s)	1411, 1896
BstAUI	TGTACA	1 cut(s)	1296
BstC8I	GCNNGC	5 cut(s)	792, 1508, 1641, 1753, 1757
BstDSI	CCRYGG	2 cut(s)	430, 2255
BstENI	CCTNNNNNAGG	1 cut(s)	302
BstF5I	GGATG	3 cut(s)	1640, 1978, 2120
BstFNI	CGCG	3 cut(s)	826, 1468, 1786
BstHHI	GCGC	5 cut(s)	826, 1470, 1786, 1954, 2462
BstKTI	GATC	8 cut(s)	10, 274, 1174, 1249, 1504, 1522, 1810, 1814
BstMAI	GTCTC	5 cut(s)	396, 546, 848, 1325, 1422
BstMBI	GATC	8 cut(s)	7, 271, 1171, 1246, 1501, 1519, 1807, 1811
BstMWI	GCNNNNNNNGC	5 cut(s)	308, 671, 703, 1566, 1586
BstNI	CCWGG	6 cut(s)	932, 1074, 1881, 2037, 2440, 2449
BstSCI	CCNGG	7 cut(s)	318, 930, 1072, 1879, 2035, 2438, 2447
BstSFI	CTRYAG	3 cut(s)	1362, 2043, 2412
BstSLI	GKGCMC	1 cut(s)	1937
BstUI	CGCG	3 cut(s)	826, 1468, 1786
BstV1I	GCAGC	6 cut(s)	497, 654, 1760, 2057, 2335, 2357
BstV2I	GAAGAC	1 cut(s)	317
BstX2I	RGATCY	2 cut(s)	271, 1171
BstYI	RGATCY	2 cut(s)	271, 1171
Bsu15I	ATCGAT	1 cut(s)	1810
BsuI	GTATCC	1 cut(s)	2409
BsuRI	GGCC	2 cut(s)	790, 1072
BsuTUI	ATCGAT	1 cut(s)	1810
BtgI	CCRYGG	2 cut(s)	430, 2255
BtsCI	GGATG	3 cut(s)	1640, 1978, 2120
BtsI	GCAGTG	1 cut(s)	381
BtsIMutI	CAGTG	9 cut(s)	10, 381, 783, 870, 1146, 1222, 1512, 1829, 2010
BveI	ACCTGC	1 cut(s)	578
Cac8I	GCNNGC	5 cut(s)	792, 1508, 1641, 1753, 1757
CaiI	CAGNNNCTG	3 cut(s)	1340, 1370, 2351
CfoI	GCGC	5 cut(s)	826, 1470, 1786, 1954, 2462
Cfr13I	GGNCC	3 cut(s)	49, 840, 2039
ClaI	ATCGAT	1 cut(s)	1810
CseI	GACGC	1 cut(s)	1885
Csp6I	GTAC	4 cut(s)	519, 558, 726, 1297
CviAII	CATG	7 cut(s)	395, 418, 584, 969, 1768, 1851, 2256
CviQI	GTAC	4 cut(s)	519, 558, 726, 1297
DpnI	GATC	8 cut(s)	9, 273, 1173, 1248, 1503, 1521, 1809, 1813
DpnII	GATC	8 cut(s)	7, 271, 1171, 1246, 1501, 1519, 1807, 1811
EaeI	YGGCCR	1 cut(s)	1070
Eam1104I	CTCTTC	1 cut(s)	1098
EarI	CTCTTC	1 cut(s)	1098
Eco130I	CCWWGG	3 cut(s)	1924, 1994, 2255
Eco31I	GGTCTC	1 cut(s)	546
Eco47I	GGWCC	3 cut(s)	49, 840, 2039
Eco57I	CTGAAG	3 cut(s)	474, 1151, 2309
EcoNI	CCTNNNNNAGG	1 cut(s)	302
EcoRII	CCWGG	6 cut(s)	930, 1072, 1879, 2035, 2438, 2447
EcoT14I	CCWWGG	3 cut(s)	1924, 1994, 2255
ErhI	CCWWGG	3 cut(s)	1924, 1994, 2255
Esp3I	CGTCTC	1 cut(s)	396
FaeI	CATG	7 cut(s)	398, 421, 587, 972, 1771, 1854, 2259
FaqI	GGGAC	2 cut(s)	449, 2273
FatI	CATG	7 cut(s)	394, 417, 583, 968, 1767, 1850, 2255
FauI	CCCGC	3 cut(s)	647, 905, 1916
FauNDI	CATATG	1 cut(s)	1973
FbaI	TGATCA	1 cut(s)	7
FblI	GTMKAC	2 cut(s)	595, 1361
Fnu4HI	GCNGC	7 cut(s)	486, 643, 951, 1749, 2046, 2346, 2349
FokI	GGATG	3 cut(s)	1647, 1965, 2107
Fsp4HI	GCNGC	7 cut(s)	486, 643, 951, 1749, 2046, 2346, 2349
FspAI	RTGCGCAY	1 cut(s)	2461
FspBI	CTAG	7 cut(s)	171, 201, 611, 1035, 1277, 2214, 2403
FspI	TGCGCA	1 cut(s)	2461
GlaI	GCGC	5 cut(s)	825, 1469, 1785, 1953, 2461
GluI	GCNGC	7 cut(s)	486, 643, 951, 1749, 2046, 2346, 2349
GsaI	CCCAGC	1 cut(s)	723
GsuI	CTGGAG	1 cut(s)	1392
HaeIII	GGCC	2 cut(s)	790, 1072
HapII	CCGG	3 cut(s)	319, 843, 1544
HgaI	GACGC	1 cut(s)	1885
HhaI	GCGC	5 cut(s)	826, 1470, 1786, 1954, 2462
Hin1I	GRCGYC	2 cut(s)	1411, 1896
Hin1II	CATG	7 cut(s)	398, 421, 587, 972, 1771, 1854, 2259
Hin6I	GCGC	5 cut(s)	824, 1468, 1784, 1952, 2460
HinP1I	GCGC	5 cut(s)	824, 1468, 1784, 1952, 2460
HincII	GTYRAC	2 cut(s)	2008, 2102
HindII	GTYRAC	2 cut(s)	2008, 2102
HpaI	GTTAAC	1 cut(s)	2008
HpaII	CCGG	3 cut(s)	319, 843, 1544
HphI	GGTGA	4 cut(s)	320, 1013, 1093, 2164
Hpy166II	GTNNAC	4 cut(s)	596, 1362, 2008, 2102
Hpy188I	TCNGA	9 cut(s)	31, 114, 134, 753, 861, 900, 1246, 1312, 2316
Hpy188III	TCNNGA	7 cut(s)	843, 926, 1169, 1209, 1289, 1371, 2066
Hpy8I	GTNNAC	4 cut(s)	596, 1362, 2008, 2102
HpyAV	CCTTC	5 cut(s)	1465, 1774, 2134, 2330, 2444
HpyCH4III	ACNGT	7 cut(s)	14, 390, 476, 562, 874, 1574, 1833
HpyCH4IV	ACGT	4 cut(s)	454, 568, 1411, 1966
HpyF10VI	GCNNNNNNNGC	5 cut(s)	308, 671, 703, 1566, 1586
HpySE526I	ACGT	4 cut(s)	454, 568, 1411, 1966
Hsp92I	GRCGYC	2 cut(s)	1411, 1896
Hsp92II	CATG	7 cut(s)	398, 421, 587, 972, 1771, 1854, 2259
HspAI	GCGC	5 cut(s)	824, 1468, 1784, 1952, 2460
Kpn2I	TCCGGA	1 cut(s)	842
Ksp22I	TGATCA	1 cut(s)	7
KspAI	GTTAAC	1 cut(s)	2008
Kzo9I	GATC	8 cut(s)	7, 271, 1171, 1246, 1501, 1519, 1807, 1811
LguI	GCTCTTC	1 cut(s)	1098
LmnI	GCTCC	3 cut(s)	135, 901, 2316
Lsp1109I	GCAGC	6 cut(s)	497, 654, 1760, 2057, 2335, 2357
LweI	GCATC	3 cut(s)	1987, 2059, 2234
MaeI	CTAG	7 cut(s)	171, 201, 611, 1035, 1277, 2214, 2403
MaeII	ACGT	4 cut(s)	454, 568, 1411, 1966
MaeIII	GTNAC	6 cut(s)	868, 926, 1019, 1412, 1706, 1883
MalI	GATC	8 cut(s)	9, 273, 1173, 1248, 1503, 1521, 1809, 1813
MboI	GATC	8 cut(s)	7, 271, 1171, 1246, 1501, 1519, 1807, 1811
MboII	GAAGA	5 cut(s)	317, 755, 817, 1085, 2302
MfeI	CAATTG	2 cut(s)	350, 1108
MflI	RGATCY	2 cut(s)	271, 1171
MhlI	GDGCHC	4 cut(s)	253, 898, 1937, 2321
MlsI	TGGCCA	1 cut(s)	1072
MluNI	TGGCCA	1 cut(s)	1072
MlyI	GAGTC	6 cut(s)	850, 877, 1367, 1592, 1788, 2067
MmeI	TCCRAC	4 cut(s)	1097, 1665, 2162, 2178
Mox20I	TGGCCA	1 cut(s)	1072
MroI	TCCGGA	1 cut(s)	842
MroXI	GAANNNNTTC	2 cut(s)	977, 1306
MscI	TGGCCA	1 cut(s)	1072
Msp20I	TGGCCA	1 cut(s)	1072
MspA1I	CMGCKG	3 cut(s)	645, 1751, 2348
MspI	CCGG	3 cut(s)	319, 843, 1544
MspR9I	CCNGG	7 cut(s)	320, 932, 1074, 1881, 2037, 2440, 2449
MunI	CAATTG	2 cut(s)	350, 1108
Mva1269I	GAATGC	2 cut(s)	1450, 2347
MvaI	CCWGG	6 cut(s)	932, 1074, 1881, 2037, 2440, 2449
MvnI	CGCG	3 cut(s)	826, 1468, 1786
MwoI	GCNNNNNNNGC	5 cut(s)	308, 671, 703, 1566, 1586
NciI	CCSGG	1 cut(s)	320
NcoI	CCATGG	1 cut(s)	2255
NdeI	CATATG	1 cut(s)	1973
NdeII	GATC	8 cut(s)	7, 271, 1171, 1246, 1501, 1519, 1807, 1811
NlaIII	CATG	7 cut(s)	398, 421, 587, 972, 1771, 1854, 2259
NlaIV	GGNNCC	4 cut(s)	1173, 1657, 1870, 2444
NmuCI	GTSAC	5 cut(s)	868, 926, 1412, 1706, 1883
NsbI	TGCGCA	1 cut(s)	2461
PciSI	GCTCTTC	1 cut(s)	1098
PctI	GAATGC	2 cut(s)	1450, 2347
PdmI	GAANNNNTTC	2 cut(s)	977, 1306
PfeI	GAWTC	8 cut(s)	78, 991, 1212, 1492, 1540, 1904, 1960, 2207
PflMI	CCANNNNNTGG	1 cut(s)	2181
PkrI	GCNGC	7 cut(s)	487, 644, 952, 1750, 2047, 2347, 2350
PleI	GAGTC	6 cut(s)	850, 877, 1366, 1591, 1788, 2066
PpsI	GAGTC	6 cut(s)	850, 877, 1366, 1591, 1788, 2066
PshBI	ATTAAT	1 cut(s)	504
PsiI	TTATAA	2 cut(s)	1398, 1734
Psp6I	CCWGG	6 cut(s)	930, 1072, 1879, 2035, 2438, 2447
PspFI	CCCAGC	1 cut(s)	719
PspGI	CCWGG	6 cut(s)	930, 1072, 1879, 2035, 2438, 2447
PspN4I	GGNNCC	4 cut(s)	1173, 1657, 1870, 2444
PspPI	GGNCC	3 cut(s)	49, 840, 2039
PstI	CTGCAG	2 cut(s)	2047, 2416
PstNI	CAGNNNCTG	3 cut(s)	1340, 1370, 2351
PsuI	RGATCY	2 cut(s)	271, 1171
PvuII	CAGCTG	3 cut(s)	645, 1751, 2348
RsaI	GTAC	4 cut(s)	520, 559, 727, 1298
RsaNI	GTAC	4 cut(s)	519, 558, 726, 1297
SapI	GCTCTTC	1 cut(s)	1098
SatI	GCNGC	7 cut(s)	486, 643, 951, 1749, 2046, 2346, 2349
Sau3AI	GATC	8 cut(s)	7, 271, 1171, 1246, 1501, 1519, 1807, 1811
Sau96I	GGNCC	3 cut(s)	49, 840, 2039
ScaI	AGTACT	1 cut(s)	520
SchI	GAGTC	6 cut(s)	850, 877, 1367, 1592, 1788, 2067
ScrFI	CCNGG	7 cut(s)	320, 932, 1074, 1881, 2037, 2440, 2449
SduI	GDGCHC	4 cut(s)	253, 898, 1937, 2321
SfaNI	GCATC	3 cut(s)	1987, 2059, 2234
SfcI	CTRYAG	3 cut(s)	1362, 2043, 2412
SinI	GGWCC	3 cut(s)	49, 840, 2039
SmlI	CTYRAG	3 cut(s)	231, 885, 2263
SmoI	CTYRAG	3 cut(s)	231, 885, 2263
SpeI	ACTAGT	2 cut(s)	170, 2213
SsiI	CCGC	7 cut(s)	509, 640, 912, 950, 1466, 1909, 2144
SspI	AATATT	1 cut(s)	850
SspMI	CTAG	7 cut(s)	171, 201, 611, 1035, 1277, 2214, 2403
StyD4I	CCNGG	7 cut(s)	318, 930, 1072, 1879, 2035, 2438, 2447
StyI	CCWWGG	3 cut(s)	1924, 1994, 2255
TaaI	ACNGT	7 cut(s)	14, 390, 476, 562, 874, 1574, 1833
TaiI	ACGT	4 cut(s)	457, 571, 1414, 1969
TaqI	TCGA	2 cut(s)	1200, 1810
TaqII	GACCGA	1 cut(s)	66
TatI	WGTACW	2 cut(s)	518, 1296
TauI	GCSGC	1 cut(s)	953
TfiI	GAWTC	8 cut(s)	78, 991, 1212, 1492, 1540, 1904, 1960, 2207
TscAI	CASTG	9 cut(s)	17, 388, 783, 877, 1146, 1222, 1519, 1836, 2017
TseFI	GTSAC	5 cut(s)	868, 926, 1412, 1706, 1883
TseI	GCWGC	6 cut(s)	485, 642, 1748, 2045, 2345, 2348
Tsp45I	GTSAC	5 cut(s)	868, 926, 1412, 1706, 1883
TspDTI	ATGAA	8 cut(s)	490, 572, 755, 1085, 1228, 1447, 1756, 2109
TspGWI	ACGGA	1 cut(s)	1070
TspRI	CASTG	9 cut(s)	17, 388, 783, 877, 1146, 1222, 1519, 1836, 2017
Van91I	CCANNNNNTGG	1 cut(s)	2181
VpaK11BI	GGWCC	3 cut(s)	49, 840, 2039
VspI	ATTAAT	1 cut(s)	504
XagI	CCTNNNNNAGG	1 cut(s)	302
XapI	RAATTY	4 cut(s)	1185, 1305, 1773, 2452
XmiI	GTMKAC	2 cut(s)	595, 1361
XmnI	GAANNNNTTC	2 cut(s)	977, 1306
XspI	CTAG	7 cut(s)	171, 201, 611, 1035, 1277, 2214, 2403
ZraI	GACGTC	1 cut(s)	1412
ZrmI	AGTACT	1 cut(s)	520

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.