Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008249

Physical Location & Seq

Genomic Coordinates Forward (+)

35609941 .. 35613161

Gene Structure 3221 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1272700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1248 bp

ATGAAGGAGAAGGCTGAGAGTTACTCTCCCAAGGGAGTTCTTGAGGACTATTTTCGGAGCTCGGATTCCGAAACGAGCTCCGAGAAAGAGCCCACGCCGGACTCAGAAACTCAGCAGAATTCGAAACAGAGTTCTAGGTGGCGTGGACTCGTTAAATTGTTGACAACTAAGTCCAAAAAACCTTTAGCCACGACTGTGCATCCGCTGCTGAAATTCTCAAAGAGAATGAGCAGCAGCATGAGGGAGATTACAGGGCCGAAATTTCAGGCAGACGATGCTGAATTGATGTACCCTTTCAAGTCACCGTGGAAGAACTTCTCCCTGTATGAGCTCCAGGCCGCAACCAAATCTTTTAGCCACGAAAATCTGATTGGAAAGGGGGGTTATGCTGAAGTTTTCAAGGGCTGTCTGTCAAATGGGGAACTTGTGGCAATCAAACGGCTAACCCGGGGACAGGAGGAGATCACCGGGGACTTCTTAGCGGAGATCGGTGTCATGGCTCATGTGAAACATCCCAATACTGCCAGGCTAATTGGGTATGGTGTTGAAGGAGGGATGCATTTAGTTCTTGAGTTGTCTACAAAAGGAAGCTTGGCTTCTGTGCTTTATGGTTCCGAGGAAGAGAAGCTCGATTGGGGCATCCGGTATAAAATTGCAATAGGTGCAGCTAAGGGTTTGCAATATCTTCATGAGGGTTGTCAAAGGAGAATCATCCACAGAGATATTAAGGCTGCAAATATTTTGCTCACCGACGATTTTGAGGCCAAGATTTGTGACTTTGGTCTTGCAAAATGGCTACCGGAGAAATGGACTCACCACACTACATCAAATTGTGAGGGCACATTTGGCTATCTTGCGCCCGAGTTCCTACTGCACGGCATAGTAGATGAGAAAACTGATGTGTTTGCGTATGGTGTGGTGCTCTTGGAACTAGTCACTGGACGACGAGCTCTAGATTACTCACAGCAAAGCCTTGTTATGTGGGCTCGGCCCTTGCTGAAGAAGAATGCAATTAAAGACTTAGTTGATCCTTCTCTAGCTGATGAGTACAACCGTCGCCAGGTGAATCTTGTACTGTTGGCTGCTTCTTTATGCATACACAAGTCCTCGATACGCCGGCCAAGTATGAGTCAGGTGGTACAACTTTTGAGTGGAGATCTTAGCTGCCTGAAGTCCGTGAAGAAAAGCATATCGATGCCAATTTTCCGGAAGACATTGCGCGAAGAGCTCATCGACACAGAAGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

416

Amino Acids

46.57

Weight (kDa)

8.66

Isoelectric Point (pI)

43.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	119 - 381	1.9e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	121 - 382	1.2e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35960 AT5G35960
fragaria_vesca	FvH4_2g24300
malus_domestica	MD03G1272700.v1.1 MD11G1293600.v1.1
prunus_persica	Prupe.8G249200_v2.0.a1
pyrus_communis	pycom03g21720 pycom11g25980
rosa_chinensis	RchiOBHm_Chr6g0292171
rosa_laevigata	RLG00000012056
rosa_multiflora	Rmu_sc0000502.1_g000005 Rmu_sc0006021.1_g000005
rosa_roxburghii	Rroxscaffold_7G00175390
rosa_rugosa	Rorug06G0017700 Rorug06G0017800 Rorug06G0017900 Rorug06G0223900 Rorug06G0224000 Rorug06G0224100 Rorug06G0224200
rosa_samantha	Rh6AG337300 Rh6DG337500
rosa_wichuraiana	Rw6G029360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	169
AccI	GTMKAC	1 cut(s)	578
AccII	CGCG	1 cut(s)	1221
AccIII	TCCGGA	1 cut(s)	1206
AciI	CCGC	3 cut(s)	203, 339, 482
AclWI	GGATC	1 cut(s)	1022
AcoI	YGGCCR	1 cut(s)	1118
AcsI	RAATTY	3 cut(s)	118, 212, 260
AcuI	CTGAAG	3 cut(s)	411, 1019, 1190
AfaI	GTAC	4 cut(s)	290, 1049, 1074, 1140
AfiI	CCNNNNNNNGG	2 cut(s)	454, 1060
AgsI	TTSAA	3 cut(s)	298, 400, 548
AhlI	ACTAGT	1 cut(s)	931
AjnI	CCWGG	3 cut(s)	333, 524, 1059
AjuI	GAANNNNNNNTTGG	2 cut(s)	354, 386
AleI	CACNNNNGTG	1 cut(s)	194
Alw21I	GWGCWC	6 cut(s)	62, 80, 333, 924, 952, 1230
AlwI	GGATC	1 cut(s)	1022
Ama87I	CYCGRG	2 cut(s)	447, 860
Aor13HI	TCCGGA	1 cut(s)	1206
AoxI	GGCC	5 cut(s)	254, 336, 762, 989, 1118
ApeKI	GCWGC	7 cut(s)	205, 231, 234, 665, 731, 1082, 1164
ApoI	RAATTY	3 cut(s)	118, 212, 260
Asp700I	GAANNNNTTC	1 cut(s)	314
AspLEI	GCGC	2 cut(s)	859, 1221
AspS9I	GGNCC	2 cut(s)	254, 990
AsuC2I	CCSGG	3 cut(s)	448, 449, 469
AsuHPI	GGTGA	5 cut(s)	294, 457, 739, 806, 1075
AsuII	TTCGAA	1 cut(s)	122
AvaI	CYCGRG	2 cut(s)	447, 860
BaeGI	GKGCMC	1 cut(s)	842
BanII	GRGCYC	7 cut(s)	62, 80, 93, 333, 952, 988, 1230
BbsI	GAAGAC	1 cut(s)	1217
Bbv12I	GWGCWC	6 cut(s)	62, 80, 333, 924, 952, 1230
BbvI	GCAGC	7 cut(s)	192, 243, 246, 677, 718, 1069, 1151
BceAI	ACGGC	2 cut(s)	455, 892
BciT130I	CCWGG	3 cut(s)	335, 526, 1061
BcnI	CCSGG	3 cut(s)	448, 449, 469
BcuI	ACTAGT	1 cut(s)	931
BfaI	CTAG	4 cut(s)	135, 932, 953, 1037
BglII	AGATCT	1 cut(s)	1156
BisI	GCNGC	8 cut(s)	206, 232, 235, 339, 666, 732, 1083, 1165
BlsI	GCNGC	8 cut(s)	207, 233, 236, 340, 667, 733, 1084, 1166
Bme1390I	CCNGG	6 cut(s)	335, 448, 449, 469, 526, 1061
BmeT110I	CYCGRG	2 cut(s)	447, 860
BmgT120I	GGNCC	2 cut(s)	254, 990
BmiI	GGNNCC	1 cut(s)	613
BmrFI	CCNGG	6 cut(s)	335, 448, 449, 469, 526, 1061
BmsI	GCATC	5 cut(s)	208, 265, 546, 648, 1185
BoxI	GACNNNNGTC	1 cut(s)	780
BpiI	GAAGAC	1 cut(s)	1217
BplI	GAGNNNNNCTC	4 cut(s)	8, 40, 10, 42
BpmI	CTGGAG	1 cut(s)	317
Bpu10I	CCTNAGC	1 cut(s)	669
Bpu14I	TTCGAA	1 cut(s)	122
BpuEI	CTTGAG	2 cut(s)	62, 590
BpuMI	CCSGG	3 cut(s)	448, 449, 469
Bsa29I	ATCGAT	1 cut(s)	1193
BsaJI	CCNNGG	6 cut(s)	30, 305, 447, 448, 468, 615
BsaWI	WCCGGW	3 cut(s)	642, 799, 1206
Bsc4I	CCNNNNNNNGG	2 cut(s)	454, 1060
Bse118I	RCCGGY	1 cut(s)	1116
Bse1I	ACTGG	1 cut(s)	943
Bse3DI	GCAATG	1 cut(s)	1214
BseAI	TCCGGA	1 cut(s)	1206
BseBI	CCWGG	3 cut(s)	335, 526, 1061
BseCI	ATCGAT	1 cut(s)	1193
BseDI	CCNNGG	6 cut(s)	30, 305, 447, 448, 468, 615
BseGI	GGATG	5 cut(s)	199, 511, 561, 639, 711
BseLI	CCNNNNNNNGG	2 cut(s)	454, 1060
BseMI	GCAATG	1 cut(s)	1214
BseMII	CTCAG	3 cut(s)	6, 117, 125
BseNI	ACTGG	1 cut(s)	943
BseRI	GAGGAG	1 cut(s)	473
BseSI	GKGCMC	1 cut(s)	842
BseXI	GCAGC	7 cut(s)	192, 243, 246, 677, 718, 1069, 1151
BsgI	GTGCAG	2 cut(s)	684, 857
Bsh1236I	CGCG	1 cut(s)	1221
BshFI	GGCC	5 cut(s)	256, 338, 764, 991, 1120
BshVI	ATCGAT	1 cut(s)	1193
BsiHKAI	GWGCWC	6 cut(s)	62, 80, 333, 924, 952, 1230
BsiHKCI	CYCGRG	2 cut(s)	447, 860
BsiSI	CCGG	7 cut(s)	98, 448, 468, 643, 800, 1117, 1207
BslFI	GGGAC	2 cut(s)	465, 485
BslI	CCNNNNNNNGG	2 cut(s)	454, 1060
BsmFI	GGGAC	2 cut(s)	465, 485
BsmI	GAATGC	1 cut(s)	1012
BsnI	GGCC	5 cut(s)	256, 338, 764, 991, 1120
BsoBI	CYCGRG	2 cut(s)	447, 860
Bsp119I	TTCGAA	1 cut(s)	122
Bsp1286I	GDGCHC	9 cut(s)	62, 80, 93, 333, 842, 924, 952, 988, 1230
Bsp13I	TCCGGA	1 cut(s)	1206
Bsp143I	GATC	4 cut(s)	462, 486, 1027, 1156
BspACI	CCGC	3 cut(s)	203, 339, 482
BspANI	GGCC	5 cut(s)	256, 338, 764, 991, 1120
BspCNI	CTCAG	3 cut(s)	7, 116, 124
BspDI	ATCGAT	1 cut(s)	1193
BspEI	TCCGGA	1 cut(s)	1206
BspFNI	CGCG	1 cut(s)	1221
BspHI	TCATGA	1 cut(s)	688
BspLI	GGNNCC	1 cut(s)	613
BspPI	GGATC	1 cut(s)	1022
BspQI	GCTCTTC	1 cut(s)	1218
BspT104I	TTCGAA	1 cut(s)	122
BsrDI	GCAATG	1 cut(s)	1214
BsrFI	RCCGGY	1 cut(s)	1116
BsrI	ACTGG	1 cut(s)	943
BssAI	RCCGGY	1 cut(s)	1116
BssECI	CCNNGG	6 cut(s)	30, 305, 447, 448, 468, 615
BssMI	GATC	4 cut(s)	462, 486, 1027, 1156
BssT1I	CCWWGG	1 cut(s)	30
Bst2UI	CCWGG	3 cut(s)	335, 526, 1061
Bst4CI	ACNGT	4 cut(s)	196, 306, 1055, 1077
Bst6I	CTCTTC	2 cut(s)	615, 1218
BstAPI	GCANNNNNTGC	3 cut(s)	205, 275, 662
BstBI	TTCGAA	1 cut(s)	122
BstC8I	GCNNGC	1 cut(s)	1118
BstDEI	CTNAG	8 cut(s)	15, 103, 111, 168, 478, 669, 1021, 1160
BstDSI	CCRYGG	1 cut(s)	305
BstF5I	GGATG	5 cut(s)	199, 511, 561, 639, 711
BstFNI	CGCG	1 cut(s)	1221
BstHHI	GCGC	2 cut(s)	859, 1221
BstKTI	GATC	4 cut(s)	465, 489, 1030, 1159
BstMBI	GATC	4 cut(s)	462, 486, 1027, 1156
BstMWI	GCNNNNNNNGC	5 cut(s)	205, 275, 662, 846, 1225
BstNI	CCWGG	3 cut(s)	335, 526, 1061
BstPAI	GACNNNNGTC	1 cut(s)	780
BstSCI	CCNGG	6 cut(s)	333, 446, 447, 467, 524, 1059
BstSLI	GKGCMC	1 cut(s)	842
BstUI	CGCG	1 cut(s)	1221
BstV1I	GCAGC	7 cut(s)	192, 243, 246, 677, 718, 1069, 1151
BstV2I	GAAGAC	1 cut(s)	1217
BstX2I	RGATCY	1 cut(s)	1156
BstYI	RGATCY	1 cut(s)	1156
Bsu15I	ATCGAT	1 cut(s)	1193
BsuRI	GGCC	5 cut(s)	256, 338, 764, 991, 1120
BsuTUI	ATCGAT	1 cut(s)	1193
BtgI	CCRYGG	1 cut(s)	305
BtsCI	GGATG	5 cut(s)	199, 511, 561, 639, 711
BtsIMutI	CAGTG	1 cut(s)	936
Cac8I	GCNNGC	1 cut(s)	1118
CciI	TCATGA	1 cut(s)	688
CfoI	GCGC	2 cut(s)	859, 1221
Cfr10I	RCCGGY	1 cut(s)	1116
Cfr13I	GGNCC	2 cut(s)	254, 990
Cfr9I	CCCGGG	1 cut(s)	447
ClaI	ATCGAT	1 cut(s)	1193
Csp6I	GTAC	4 cut(s)	289, 1048, 1073, 1139
CviAII	CATG	4 cut(s)	238, 496, 503, 689
CviQI	GTAC	4 cut(s)	289, 1048, 1073, 1139
DdeI	CTNAG	8 cut(s)	15, 103, 111, 168, 478, 669, 1021, 1160
DpnI	GATC	4 cut(s)	464, 488, 1029, 1158
DpnII	GATC	4 cut(s)	462, 486, 1027, 1156
DrdI	GACNNNNNNGTC	1 cut(s)	169
DseDI	GACNNNNNNGTC	1 cut(s)	169
EaeI	YGGCCR	1 cut(s)	1118
Eam1104I	CTCTTC	2 cut(s)	615, 1218
EarI	CTCTTC	2 cut(s)	615, 1218
Ecl136II	GAGCTC	5 cut(s)	60, 78, 331, 950, 1228
Eco130I	CCWWGG	1 cut(s)	30
Eco24I	GRGCYC	7 cut(s)	62, 80, 93, 333, 952, 988, 1230
Eco53kI	GAGCTC	5 cut(s)	60, 78, 331, 950, 1228
Eco57I	CTGAAG	3 cut(s)	411, 1019, 1190
Eco88I	CYCGRG	2 cut(s)	447, 860
EcoICRI	GAGCTC	5 cut(s)	60, 78, 331, 950, 1228
EcoRI	GAATTC	1 cut(s)	118
EcoRII	CCWGG	3 cut(s)	333, 524, 1059
EcoT14I	CCWWGG	1 cut(s)	30
EcoT22I	ATGCAT	2 cut(s)	561, 1097
EcoT38I	GRGCYC	7 cut(s)	62, 80, 93, 333, 952, 988, 1230
ErhI	CCWWGG	1 cut(s)	30
FaeI	CATG	4 cut(s)	241, 499, 506, 692
FalI	AAGNNNNNCTT	2 cut(s)	580, 612
FaqI	GGGAC	2 cut(s)	465, 485
FatI	CATG	4 cut(s)	237, 495, 502, 688
FblI	GTMKAC	1 cut(s)	578
Fnu4HI	GCNGC	8 cut(s)	206, 232, 235, 339, 666, 732, 1083, 1165
FokI	GGATG	5 cut(s)	186, 498, 568, 626, 698
FriOI	GRGCYC	7 cut(s)	62, 80, 93, 333, 952, 988, 1230
Fsp4HI	GCNGC	8 cut(s)	206, 232, 235, 339, 666, 732, 1083, 1165
FspBI	CTAG	4 cut(s)	135, 932, 953, 1037
GlaI	GCGC	2 cut(s)	858, 1220
GluI	GCNGC	8 cut(s)	206, 232, 235, 339, 666, 732, 1083, 1165
GsuI	CTGGAG	1 cut(s)	317
HaeIII	GGCC	5 cut(s)	256, 338, 764, 991, 1120
HapII	CCGG	7 cut(s)	98, 448, 468, 643, 800, 1117, 1207
HhaI	GCGC	2 cut(s)	859, 1221
Hin1II	CATG	4 cut(s)	241, 499, 506, 692
Hin6I	GCGC	2 cut(s)	857, 1219
HinP1I	GCGC	2 cut(s)	857, 1219
HincII	GTYRAC	1 cut(s)	162
HindII	GTYRAC	1 cut(s)	162
HindIII	AAGCTT	1 cut(s)	589
HinfI	GANTC	7 cut(s)	65, 101, 147, 708, 811, 1066, 1129
HpaII	CCGG	7 cut(s)	98, 448, 468, 643, 800, 1117, 1207
HphI	GGTGA	5 cut(s)	294, 457, 739, 806, 1075
Hpy166II	GTNNAC	3 cut(s)	146, 162, 579
Hpy188I	TCNGA	7 cut(s)	57, 64, 70, 82, 106, 369, 616
Hpy188III	TCNNGA	5 cut(s)	41, 569, 689, 953, 1207
Hpy8I	GTNNAC	3 cut(s)	146, 162, 579
Hpy99I	CGWCG	3 cut(s)	755, 948, 1059
HpyAV	CCTTC	3 cut(s)	4, 542, 1041
HpyCH4III	ACNGT	4 cut(s)	196, 306, 1055, 1077
HpyF10VI	GCNNNNNNNGC	5 cut(s)	205, 275, 662, 846, 1225
HpyF3I	CTNAG	8 cut(s)	15, 103, 111, 168, 478, 669, 1021, 1160
Hsp92II	CATG	4 cut(s)	241, 499, 506, 692
HspAI	GCGC	2 cut(s)	857, 1219
Kpn2I	TCCGGA	1 cut(s)	1206
KroI	GCCGGC	1 cut(s)	1116
KroNI	GCCGGC	1 cut(s)	1118
Kzo9I	GATC	4 cut(s)	462, 486, 1027, 1156
LguI	GCTCTTC	1 cut(s)	1218
LmnI	GCTCC	3 cut(s)	57, 83, 336
Lsp1109I	GCAGC	7 cut(s)	192, 243, 246, 677, 718, 1069, 1151
LweI	GCATC	5 cut(s)	208, 265, 546, 648, 1185
MaeI	CTAG	4 cut(s)	135, 932, 953, 1037
MaeIII	GTNAC	4 cut(s)	20, 300, 773, 934
MalI	GATC	4 cut(s)	464, 488, 1029, 1158
MboI	GATC	4 cut(s)	462, 486, 1027, 1156
MboII	GAAGA	8 cut(s)	322, 632, 677, 1012, 1015, 1192, 1222, 1235
MflI	RGATCY	1 cut(s)	1156
MhlI	GDGCHC	9 cut(s)	62, 80, 93, 333, 842, 924, 952, 988, 1230
MlyI	GAGTC	4 cut(s)	95, 141, 805, 1138
MnlI	CCTC	9 cut(s)	37, 234, 451, 545, 610, 685, 754, 829, 1117
Mph1103I	ATGCAT	2 cut(s)	561, 1097
MroI	TCCGGA	1 cut(s)	1206
MroNI	GCCGGC	1 cut(s)	1116
MroXI	GAANNNNTTC	1 cut(s)	314
MseI	TTAA	3 cut(s)	153, 726, 1014
MslI	CAYNNNNRTG	2 cut(s)	194, 1193
MspA1I	CMGCKG	1 cut(s)	205
MspI	CCGG	7 cut(s)	98, 448, 468, 643, 800, 1117, 1207
MspR9I	CCNGG	6 cut(s)	335, 448, 449, 469, 526, 1061
Mva1269I	GAATGC	1 cut(s)	1012
MvaI	CCWGG	3 cut(s)	335, 526, 1061
MvnI	CGCG	1 cut(s)	1221
MwoI	GCNNNNNNNGC	5 cut(s)	205, 275, 662, 846, 1225
NaeI	GCCGGC	1 cut(s)	1118
NciI	CCSGG	3 cut(s)	448, 449, 469
NdeII	GATC	4 cut(s)	462, 486, 1027, 1156
NgoMIV	GCCGGC	1 cut(s)	1116
NlaIII	CATG	4 cut(s)	241, 499, 506, 692
NlaIV	GGNNCC	1 cut(s)	613
NmeAIII	GCCGAG	1 cut(s)	967
NmuCI	GTSAC	3 cut(s)	300, 773, 934
NsiI	ATGCAT	2 cut(s)	561, 1097
NspV	TTCGAA	1 cut(s)	122
OliI	CACNNNNGTG	1 cut(s)	194
PagI	TCATGA	1 cut(s)	688
PciSI	GCTCTTC	1 cut(s)	1218
PctI	GAATGC	1 cut(s)	1012
PdiI	GCCGGC	1 cut(s)	1118
PdmI	GAANNNNTTC	1 cut(s)	314
PfeI	GAWTC	3 cut(s)	65, 708, 1066
PkrI	GCNGC	8 cut(s)	207, 233, 236, 340, 667, 733, 1084, 1166
PleI	GAGTC	4 cut(s)	95, 141, 805, 1137
PpsI	GAGTC	4 cut(s)	95, 141, 805, 1137
PshAI	GACNNNNGTC	1 cut(s)	780
Psp124BI	GAGCTC	5 cut(s)	62, 80, 333, 952, 1230
Psp6I	CCWGG	3 cut(s)	333, 524, 1059
PspGI	CCWGG	3 cut(s)	333, 524, 1059
PspN4I	GGNNCC	1 cut(s)	613
PspPI	GGNCC	2 cut(s)	254, 990
PsuI	RGATCY	1 cut(s)	1156
RsaI	GTAC	4 cut(s)	290, 1049, 1074, 1140
RsaNI	GTAC	4 cut(s)	289, 1048, 1073, 1139
RseI	CAYNNNNRTG	2 cut(s)	194, 1193
SacI	GAGCTC	5 cut(s)	62, 80, 333, 952, 1230
SapI	GCTCTTC	1 cut(s)	1218
SaqAI	TTAA	3 cut(s)	153, 726, 1014
SatI	GCNGC	8 cut(s)	206, 232, 235, 339, 666, 732, 1083, 1165
Sau3AI	GATC	4 cut(s)	462, 486, 1027, 1156
Sau96I	GGNCC	2 cut(s)	254, 990
SchI	GAGTC	4 cut(s)	95, 141, 805, 1138
ScrFI	CCNGG	6 cut(s)	335, 448, 449, 469, 526, 1061
SduI	GDGCHC	9 cut(s)	62, 80, 93, 333, 842, 924, 952, 988, 1230
SfaNI	GCATC	5 cut(s)	208, 265, 546, 648, 1185
SfuI	TTCGAA	1 cut(s)	122
SmaI	CCCGGG	1 cut(s)	449
SmiMI	CAYNNNNRTG	2 cut(s)	194, 1193
SmlI	CTYRAG	2 cut(s)	41, 569
SmoI	CTYRAG	2 cut(s)	41, 569
SpeI	ACTAGT	1 cut(s)	931
SsiI	CCGC	3 cut(s)	203, 339, 482
SspI	AATATT	1 cut(s)	739
SspMI	CTAG	4 cut(s)	135, 932, 953, 1037
SstI	GAGCTC	5 cut(s)	62, 80, 333, 952, 1230
StyD4I	CCNGG	6 cut(s)	333, 446, 447, 467, 524, 1059
StyI	CCWWGG	1 cut(s)	30
TaaI	ACNGT	4 cut(s)	196, 306, 1055, 1077
TaqI	TCGA	5 cut(s)	122, 630, 1109, 1193, 1233
TatI	WGTACW	2 cut(s)	1047, 1072
TauI	GCSGC	1 cut(s)	341
TfiI	GAWTC	3 cut(s)	65, 708, 1066
Tru1I	TTAA	3 cut(s)	153, 726, 1014
Tru9I	TTAA	3 cut(s)	153, 726, 1014
TscAI	CASTG	1 cut(s)	943
TseFI	GTSAC	3 cut(s)	300, 773, 934
TseI	GCWGC	7 cut(s)	205, 231, 234, 665, 731, 1082, 1164
Tsp45I	GTSAC	3 cut(s)	300, 773, 934
TspDTI	ATGAA	2 cut(s)	17, 677
TspGWI	ACGGA	1 cut(s)	1165
TspMI	CCCGGG	1 cut(s)	447
TspRI	CASTG	1 cut(s)	943
XapI	RAATTY	3 cut(s)	118, 212, 260
XbaI	TCTAGA	1 cut(s)	952
XmaI	CCCGGG	1 cut(s)	447
XmiI	GTMKAC	1 cut(s)	578
XmnI	GAANNNNTTC	1 cut(s)	314
XspI	CTAG	4 cut(s)	135, 932, 953, 1037
Zsp2I	ATGCAT	2 cut(s)	561, 1097

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.