Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0008249

Physical Location & Seq

Genomic Coordinates Forward (+)

41289580 .. 41292578

Gene Structure 2999 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1293600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGGAGGAGAAGGCTGATGCTTACTCTCCCAAGGGAGTTCTTGAGGACTATTTTCGGAGCTCGGATTCCGAAACGAGCTCCGAGAAAGAGCCCACGCCGGATTCAGAAGCTAACCAGAATTCAAAACAGAGTTCTAGGTGGGGTGGATTTGTTAAATTGTTGACATCCAAGTCCAAAAACCCTTTAGCCACGACAGTGCGTCCGCTGCGGAAATTCTCAAAGAGAATGAGCAGCAGCATGAGGGAGATTATCGGGCCGAAATTTCAGGCAGATGATGCTGAAATGCTGTACCCTTTCAAGTCACCGTGGAAGAACTTCTCCCTGTATGAGCTGCAGGCCGCAACCAAATCTTTTAGCCACGAAAATCTGATTGGAAAGGGGGGTTATGCTGAAGTTTTTAAGGGCTGTCTGCAAAATGGGCAACCTGTGGCAATCAAGCGGCTAACCCGGGGAACGCAGGAGGAGATCACCGGCGACTTCTTGGCGGAGATCGGTATCATGGCTCATGTGAATCATCCTAATACTGCCAGGCTAATTGGGTATGGGGTTGAAGGTGGCATGCATTTGGTTCTTGAGCTGTCTACAAAGGGAAGCTTGGCTTCTGTCCTTTATGCTTCGGAGGAAGAGAAACTCGATTGGGGCATCCGGTATAGAATTGCAGTGGGTGCAGCAAAGGGTTTGCAATATCTTCATGAGGGTTGTCAAAGGAGAATTATCCACAGAGATATTAAGGCTGCAAATATTTTGCTCACCGACGATTTTGAGGCCAAGATTTGTGACTTTGGTCTTGCAAAATGGCTACCGGAGAAATGGACACACCACACTGTATCAAATTTTGAGGGCACATTTGGCTATCTTGCGCCCGAGTTCCTACTGCACGGCATAGTAGATGAGAAAACTGATGTGTTTGCGTATGGTGTGGTGCTCTTGGAACTAGTCACTGGACGACGAGCTCTAGATTACTCGCAGCAAAGCCTTGTTATGTGGGCAAGGCCCTTGCTGAAGAAGAATTCAATCAAAGACTTGGTTGATCCTTCTCTAGCTGATGAGTACAACCGTCGCGAGATGAATCTCGTATTGTTGGCTGCTTCTCTATGCATACACAAGTCCTCGACACGCCGGCCAAGTATGAGTCAGGTGGTACAGCTTATGAGTGGAGATCTTAGCTGCCTGAAGTCCTTGAAGAAAAGCATATCAATGCCAATTTTCCGGAATGCATTGCGTGAAGAGCTCATCGACACAGAAGACCGTAACGCAACCATTCGGACGGGTTCTCAAACCATGGAAGCCGGTAAAGTAACGGGACACGGAAACTTGGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

441

Amino Acids

49.21

Weight (kDa)

8.12

Isoelectric Point (pI)

44.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	119 - 382	3.1e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	121 - 382	8.5e-43	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35960 AT5G35960
fragaria_vesca	FvH4_2g24300
malus_domestica	MD03G1272700.v1.1 MD11G1293600.v1.1
prunus_persica	Prupe.8G249200_v2.0.a1
pyrus_communis	pycom03g21720 pycom11g25980
rosa_chinensis	RchiOBHm_Chr6g0292171
rosa_laevigata	RLG00000012056
rosa_multiflora	Rmu_sc0000502.1_g000005 Rmu_sc0006021.1_g000005
rosa_roxburghii	Rroxscaffold_7G00175390
rosa_rugosa	Rorug06G0017700 Rorug06G0017800 Rorug06G0017900 Rorug06G0223900 Rorug06G0224000 Rorug06G0224100 Rorug06G0224200
rosa_samantha	Rh6AG337300 Rh6DG337500
rosa_wichuraiana	Rw6G029360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	169
AccI	GTMKAC	1 cut(s)	581
AccII	CGCG	1 cut(s)	1062
AccIII	TCCGGA	1 cut(s)	1209
AciI	CCGC	5 cut(s)	203, 208, 339, 439, 485
AclWI	GGATC	1 cut(s)	1025
AcoI	YGGCCR	1 cut(s)	1121
AcsI	RAATTY	5 cut(s)	118, 212, 260, 832, 1009
AcuI	CTGAAG	3 cut(s)	411, 1022, 1193
AfaI	GTAC	3 cut(s)	290, 1052, 1143
AgsI	TTSAA	5 cut(s)	123, 298, 551, 1014, 1183
AhdI	GACNNNNNGTC	1 cut(s)	198
AhlI	ACTAGT	1 cut(s)	934
AjnI	CCWGG	1 cut(s)	527
AjuI	GAANNNNNNNTTGG	2 cut(s)	354, 386
AleI	CACNNNNGTG	1 cut(s)	194
Alw21I	GWGCWC	5 cut(s)	62, 80, 927, 955, 1233
AlwI	GGATC	1 cut(s)	1025
Ama87I	CYCGRG	2 cut(s)	447, 863
Aor13HI	TCCGGA	1 cut(s)	1209
AoxI	GGCC	5 cut(s)	254, 336, 765, 992, 1121
ApeKI	GCWGC	9 cut(s)	205, 231, 234, 331, 668, 734, 967, 1085, 1167
ApoI	RAATTY	5 cut(s)	118, 212, 260, 832, 1009
Asp700I	GAANNNNTTC	1 cut(s)	314
AspLEI	GCGC	1 cut(s)	862
AspS9I	GGNCC	2 cut(s)	254, 993
AsuC2I	CCSGG	2 cut(s)	448, 449
AsuHPI	GGTGA	3 cut(s)	294, 460, 742
AvaI	CYCGRG	2 cut(s)	447, 863
BaeGI	GKGCMC	1 cut(s)	845
BanII	GRGCYC	5 cut(s)	62, 80, 93, 955, 1233
BbsI	GAAGAC	1 cut(s)	1251
Bbv12I	GWGCWC	5 cut(s)	62, 80, 927, 955, 1233
BbvI	GCAGC	9 cut(s)	192, 243, 246, 318, 680, 721, 979, 1072, 1154
BceAI	ACGGC	1 cut(s)	895
BciT130I	CCWGG	1 cut(s)	529
BcnI	CCSGG	2 cut(s)	448, 449
BcuI	ACTAGT	1 cut(s)	934
BfaI	CTAG	4 cut(s)	135, 935, 956, 1040
BfmI	CTRYAG	1 cut(s)	332
BglII	AGATCT	1 cut(s)	1159
Bme1390I	CCNGG	3 cut(s)	448, 449, 529
BmeRI	GACNNNNNGTC	1 cut(s)	198
BmeT110I	CYCGRG	2 cut(s)	447, 863
BmgT120I	GGNCC	2 cut(s)	254, 993
BmrFI	CCNGG	3 cut(s)	448, 449, 529
BmsI	GCATC	3 cut(s)	7, 265, 651
BoxI	GACNNNNGTC	1 cut(s)	783
BpiI	GAAGAC	1 cut(s)	1251
BpuEI	CTTGAG	2 cut(s)	62, 593
BpuMI	CCSGG	2 cut(s)	448, 449
BsaBI	GATNNNNATC	1 cut(s)	494
BsaJI	CCNNGG	5 cut(s)	30, 305, 447, 448, 1281
BsaWI	WCCGGW	3 cut(s)	645, 802, 1209
Bse118I	RCCGGY	3 cut(s)	470, 1119, 1289
Bse1I	ACTGG	1 cut(s)	946
Bse3DI	GCAATG	1 cut(s)	1217
Bse8I	GATNNNNATC	1 cut(s)	494
BseAI	TCCGGA	1 cut(s)	1209
BseBI	CCWGG	1 cut(s)	529
BseDI	CCNNGG	5 cut(s)	30, 305, 447, 448, 1281
BseGI	GGATG	3 cut(s)	164, 514, 642
BseJI	GATNNNNATC	1 cut(s)	494
BseMI	GCAATG	1 cut(s)	1217
BseNI	ACTGG	1 cut(s)	946
BseRI	GAGGAG	2 cut(s)	20, 476
BseSI	GKGCMC	1 cut(s)	845
BseXI	GCAGC	9 cut(s)	192, 243, 246, 318, 680, 721, 979, 1072, 1154
BsgI	GTGCAG	2 cut(s)	687, 860
Bsh1236I	CGCG	1 cut(s)	1062
BshFI	GGCC	5 cut(s)	256, 338, 767, 994, 1123
BsiHKAI	GWGCWC	5 cut(s)	62, 80, 927, 955, 1233
BsiHKCI	CYCGRG	2 cut(s)	447, 863
BsiSI	CCGG	8 cut(s)	98, 448, 471, 646, 803, 1120, 1210, 1290
BslFI	GGGAC	1 cut(s)	1317
BsmFI	GGGAC	1 cut(s)	1317
BsmI	GAATGC	1 cut(s)	1219
BsnI	GGCC	5 cut(s)	256, 338, 767, 994, 1123
BsoBI	CYCGRG	2 cut(s)	447, 863
Bsp1286I	GDGCHC	7 cut(s)	62, 80, 93, 845, 927, 955, 1233
Bsp13I	TCCGGA	1 cut(s)	1209
Bsp143I	GATC	4 cut(s)	465, 489, 1030, 1159
Bsp19I	CCATGG	1 cut(s)	1281
Bsp68I	TCGCGA	1 cut(s)	1062
BspACI	CCGC	5 cut(s)	203, 208, 339, 439, 485
BspANI	GGCC	5 cut(s)	256, 338, 767, 994, 1123
BspEI	TCCGGA	1 cut(s)	1209
BspFNI	CGCG	1 cut(s)	1062
BspHI	TCATGA	1 cut(s)	691
BspMAI	CTGCAG	1 cut(s)	336
BspPI	GGATC	1 cut(s)	1025
BspQI	GCTCTTC	1 cut(s)	1221
BsrDI	GCAATG	1 cut(s)	1217
BsrFI	RCCGGY	3 cut(s)	470, 1119, 1289
BsrI	ACTGG	1 cut(s)	946
BssAI	RCCGGY	3 cut(s)	470, 1119, 1289
BssECI	CCNNGG	5 cut(s)	30, 305, 447, 448, 1281
BssMI	GATC	4 cut(s)	465, 489, 1030, 1159
BssT1I	CCWWGG	2 cut(s)	30, 1281
Bst2UI	CCWGG	1 cut(s)	529
Bst4CI	ACNGT	5 cut(s)	196, 306, 826, 1058, 1250
Bst6I	CTCTTC	2 cut(s)	618, 1221
BstAPI	GCANNNNNTGC	2 cut(s)	275, 665
BstC8I	GCNNGC	3 cut(s)	336, 560, 1121
BstDEI	CTNAG	1 cut(s)	1163
BstDSI	CCRYGG	2 cut(s)	305, 1281
BstF5I	GGATG	3 cut(s)	164, 514, 642
BstFNI	CGCG	1 cut(s)	1062
BstHHI	GCGC	1 cut(s)	862
BstKTI	GATC	4 cut(s)	468, 492, 1033, 1162
BstMBI	GATC	4 cut(s)	465, 489, 1030, 1159
BstMWI	GCNNNNNNNGC	6 cut(s)	205, 275, 418, 665, 849, 1228
BstNI	CCWGG	1 cut(s)	529
BstNSI	RCATGY	1 cut(s)	562
BstPAI	GACNNNNGTC	1 cut(s)	783
BstSCI	CCNGG	3 cut(s)	446, 447, 527
BstSFI	CTRYAG	1 cut(s)	332
BstSLI	GKGCMC	1 cut(s)	845
BstUI	CGCG	1 cut(s)	1062
BstV1I	GCAGC	9 cut(s)	192, 243, 246, 318, 680, 721, 979, 1072, 1154
BstV2I	GAAGAC	1 cut(s)	1251
BstX2I	RGATCY	1 cut(s)	1159
BstYI	RGATCY	1 cut(s)	1159
BsuRI	GGCC	5 cut(s)	256, 338, 767, 994, 1123
BtgI	CCRYGG	2 cut(s)	305, 1281
BtsCI	GGATG	3 cut(s)	164, 514, 642
BtsI	GCAGTG	1 cut(s)	666
BtsIMutI	CAGTG	4 cut(s)	201, 666, 822, 939
BtuMI	TCGCGA	1 cut(s)	1062
Cac8I	GCNNGC	3 cut(s)	336, 560, 1121
CciI	TCATGA	1 cut(s)	691
CfoI	GCGC	1 cut(s)	862
Cfr10I	RCCGGY	3 cut(s)	470, 1119, 1289
Cfr13I	GGNCC	2 cut(s)	254, 993
Cfr9I	CCCGGG	1 cut(s)	447
CseI	GACGC	1 cut(s)	188
Csp6I	GTAC	3 cut(s)	289, 1051, 1142
CviAII	CATG	6 cut(s)	238, 499, 506, 559, 692, 1282
CviQI	GTAC	3 cut(s)	289, 1051, 1142
DdeI	CTNAG	1 cut(s)	1163
DpnI	GATC	4 cut(s)	467, 491, 1032, 1161
DpnII	GATC	4 cut(s)	465, 489, 1030, 1159
DrdI	GACNNNNNNGTC	1 cut(s)	169
DriI	GACNNNNNGTC	1 cut(s)	198
DseDI	GACNNNNNNGTC	1 cut(s)	169
EaeI	YGGCCR	1 cut(s)	1121
Eam1104I	CTCTTC	2 cut(s)	618, 1221
Eam1105I	GACNNNNNGTC	1 cut(s)	198
EarI	CTCTTC	2 cut(s)	618, 1221
EciI	GGCGGA	1 cut(s)	500
Ecl136II	GAGCTC	4 cut(s)	60, 78, 953, 1231
Eco130I	CCWWGG	2 cut(s)	30, 1281
Eco24I	GRGCYC	5 cut(s)	62, 80, 93, 955, 1233
Eco53kI	GAGCTC	4 cut(s)	60, 78, 953, 1231
Eco57I	CTGAAG	3 cut(s)	411, 1022, 1193
Eco88I	CYCGRG	2 cut(s)	447, 863
EcoICRI	GAGCTC	4 cut(s)	60, 78, 953, 1231
EcoO109I	RGGNCCY	1 cut(s)	993
EcoRI	GAATTC	2 cut(s)	118, 1009
EcoRII	CCWGG	1 cut(s)	527
EcoT14I	CCWWGG	2 cut(s)	30, 1281
EcoT22I	ATGCAT	3 cut(s)	564, 1100, 1219
EcoT38I	GRGCYC	5 cut(s)	62, 80, 93, 955, 1233
ErhI	CCWWGG	2 cut(s)	30, 1281
FaeI	CATG	6 cut(s)	241, 502, 509, 562, 695, 1285
FalI	AAGNNNNNCTT	4 cut(s)	578, 610, 583, 615
FaqI	GGGAC	1 cut(s)	1317
FatI	CATG	6 cut(s)	237, 498, 505, 558, 691, 1281
FblI	GTMKAC	1 cut(s)	581
FokI	GGATG	3 cut(s)	151, 501, 629
FriOI	GRGCYC	5 cut(s)	62, 80, 93, 955, 1233
FspBI	CTAG	4 cut(s)	135, 935, 956, 1040
GlaI	GCGC	1 cut(s)	861
HaeIII	GGCC	5 cut(s)	256, 338, 767, 994, 1123
HapII	CCGG	8 cut(s)	98, 448, 471, 646, 803, 1120, 1210, 1290
HgaI	GACGC	1 cut(s)	188
HhaI	GCGC	1 cut(s)	862
Hin1II	CATG	6 cut(s)	241, 502, 509, 562, 695, 1285
Hin6I	GCGC	1 cut(s)	860
HinP1I	GCGC	1 cut(s)	860
HincII	GTYRAC	1 cut(s)	162
HindII	GTYRAC	1 cut(s)	162
HindIII	AAGCTT	1 cut(s)	592
HinfI	GANTC	5 cut(s)	65, 101, 511, 1069, 1132
HpaII	CCGG	8 cut(s)	98, 448, 471, 646, 803, 1120, 1210, 1290
HphI	GGTGA	3 cut(s)	294, 460, 742
Hpy166II	GTNNAC	2 cut(s)	162, 582
Hpy188I	TCNGA	8 cut(s)	57, 64, 70, 82, 106, 369, 619, 1266
Hpy188III	TCNNGA	6 cut(s)	41, 572, 692, 956, 1061, 1210
Hpy8I	GTNNAC	2 cut(s)	162, 582
Hpy99I	CGWCG	3 cut(s)	758, 951, 1062
HpyAV	CCTTC	3 cut(s)	4, 545, 1044
HpyCH4III	ACNGT	5 cut(s)	196, 306, 826, 1058, 1250
HpyF10VI	GCNNNNNNNGC	6 cut(s)	205, 275, 418, 665, 849, 1228
HpyF3I	CTNAG	1 cut(s)	1163
Hsp92II	CATG	6 cut(s)	241, 502, 509, 562, 695, 1285
HspAI	GCGC	1 cut(s)	860
Kpn2I	TCCGGA	1 cut(s)	1209
KroI	GCCGGC	1 cut(s)	1119
KroNI	GCCGGC	1 cut(s)	1121
Kzo9I	GATC	4 cut(s)	465, 489, 1030, 1159
LguI	GCTCTTC	1 cut(s)	1221
LmnI	GCTCC	2 cut(s)	57, 83
Lsp1109I	GCAGC	9 cut(s)	192, 243, 246, 318, 680, 721, 979, 1072, 1154
LweI	GCATC	3 cut(s)	7, 265, 651
MaeI	CTAG	4 cut(s)	135, 935, 956, 1040
MaeIII	GTNAC	5 cut(s)	300, 776, 937, 1250, 1297
MalI	GATC	4 cut(s)	467, 491, 1032, 1161
MboI	GATC	4 cut(s)	465, 489, 1030, 1159
MboII	GAAGA	8 cut(s)	322, 635, 680, 1015, 1018, 1195, 1238, 1256
MflI	RGATCY	1 cut(s)	1159
MhlI	GDGCHC	7 cut(s)	62, 80, 93, 845, 927, 955, 1233
MlyI	GAGTC	1 cut(s)	1141
MnlI	CCTC	8 cut(s)	37, 234, 454, 613, 688, 757, 832, 1120
Mph1103I	ATGCAT	3 cut(s)	564, 1100, 1219
MroI	TCCGGA	1 cut(s)	1209
MroNI	GCCGGC	1 cut(s)	1119
MroXI	GAANNNNTTC	1 cut(s)	314
MseI	TTAA	3 cut(s)	153, 399, 729
MslI	CAYNNNNRTG	2 cut(s)	194, 1196
MspA1I	CMGCKG	1 cut(s)	205
MspI	CCGG	8 cut(s)	98, 448, 471, 646, 803, 1120, 1210, 1290
MspR9I	CCNGG	3 cut(s)	448, 449, 529
Mva1269I	GAATGC	1 cut(s)	1219
MvaI	CCWGG	1 cut(s)	529
MvnI	CGCG	1 cut(s)	1062
MwoI	GCNNNNNNNGC	6 cut(s)	205, 275, 418, 665, 849, 1228
NaeI	GCCGGC	1 cut(s)	1121
NciI	CCSGG	2 cut(s)	448, 449
NcoI	CCATGG	1 cut(s)	1281
NdeII	GATC	4 cut(s)	465, 489, 1030, 1159
NgoMIV	GCCGGC	1 cut(s)	1119
NlaIII	CATG	6 cut(s)	241, 502, 509, 562, 695, 1285
NmuCI	GTSAC	3 cut(s)	300, 776, 937
NruI	TCGCGA	1 cut(s)	1062
NsiI	ATGCAT	3 cut(s)	564, 1100, 1219
NspI	RCATGY	1 cut(s)	562
OliI	CACNNNNGTG	1 cut(s)	194
PaeI	GCATGC	1 cut(s)	562
PagI	TCATGA	1 cut(s)	691
PciSI	GCTCTTC	1 cut(s)	1221
PctI	GAATGC	1 cut(s)	1219
PdiI	GCCGGC	1 cut(s)	1121
PdmI	GAANNNNTTC	1 cut(s)	314
PfeI	GAWTC	4 cut(s)	65, 101, 511, 1069
PleI	GAGTC	1 cut(s)	1140
PpsI	GAGTC	1 cut(s)	1140
PshAI	GACNNNNGTC	1 cut(s)	783
Psp124BI	GAGCTC	4 cut(s)	62, 80, 955, 1233
Psp6I	CCWGG	1 cut(s)	527
PspGI	CCWGG	1 cut(s)	527
PspPI	GGNCC	2 cut(s)	254, 993
PstI	CTGCAG	1 cut(s)	336
PsuI	RGATCY	1 cut(s)	1159
RruI	TCGCGA	1 cut(s)	1062
RsaI	GTAC	3 cut(s)	290, 1052, 1143
RsaNI	GTAC	3 cut(s)	289, 1051, 1142
RseI	CAYNNNNRTG	2 cut(s)	194, 1196
SacI	GAGCTC	4 cut(s)	62, 80, 955, 1233
SapI	GCTCTTC	1 cut(s)	1221
SaqAI	TTAA	3 cut(s)	153, 399, 729
Sau3AI	GATC	4 cut(s)	465, 489, 1030, 1159
Sau96I	GGNCC	2 cut(s)	254, 993
SchI	GAGTC	1 cut(s)	1141
ScrFI	CCNGG	3 cut(s)	448, 449, 529
SduI	GDGCHC	7 cut(s)	62, 80, 93, 845, 927, 955, 1233
SfaNI	GCATC	3 cut(s)	7, 265, 651
SfcI	CTRYAG	1 cut(s)	332
SgrAI	CRCCGGYG	1 cut(s)	470
SmaI	CCCGGG	1 cut(s)	449
SmiMI	CAYNNNNRTG	2 cut(s)	194, 1196
SmlI	CTYRAG	2 cut(s)	41, 572
SmoI	CTYRAG	2 cut(s)	41, 572
SpeI	ACTAGT	1 cut(s)	934
SphI	GCATGC	1 cut(s)	562
SsiI	CCGC	5 cut(s)	203, 208, 339, 439, 485
SspI	AATATT	1 cut(s)	742
SspMI	CTAG	4 cut(s)	135, 935, 956, 1040
SstI	GAGCTC	4 cut(s)	62, 80, 955, 1233
StyD4I	CCNGG	3 cut(s)	446, 447, 527
StyI	CCWWGG	2 cut(s)	30, 1281
TaaI	ACNGT	5 cut(s)	196, 306, 826, 1058, 1250
TaqI	TCGA	3 cut(s)	633, 1112, 1236
TatI	WGTACW	1 cut(s)	1050
TauI	GCSGC	2 cut(s)	341, 442
TfiI	GAWTC	4 cut(s)	65, 101, 511, 1069
Tru1I	TTAA	3 cut(s)	153, 399, 729
Tru9I	TTAA	3 cut(s)	153, 399, 729
TscAI	CASTG	4 cut(s)	201, 666, 829, 946
TseFI	GTSAC	3 cut(s)	300, 776, 937
TseI	GCWGC	9 cut(s)	205, 231, 234, 331, 668, 734, 967, 1085, 1167
Tsp45I	GTSAC	3 cut(s)	300, 776, 937
TspDTI	ATGAA	2 cut(s)	680, 1082
TspGWI	ACGGA	1 cut(s)	1323
TspMI	CCCGGG	1 cut(s)	447
TspRI	CASTG	4 cut(s)	201, 666, 829, 946
XapI	RAATTY	5 cut(s)	118, 212, 260, 832, 1009
XbaI	TCTAGA	1 cut(s)	955
XceI	RCATGY	1 cut(s)	562
XmaI	CCCGGG	1 cut(s)	447
XmiI	GTMKAC	1 cut(s)	581
XmnI	GAANNNNTTC	1 cut(s)	314
XspI	CTAG	4 cut(s)	135, 935, 956, 1040
Zsp2I	ATGCAT	3 cut(s)	564, 1100, 1219

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.