AT5G38630

transmembrane ascorbate ferrireductase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0017157
Physical Location & Seq
Forward (+)
15465992 .. 15468185
2194 bp
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UTR
Exon/CDS
Intron
AT5G38630.1

Sequence Viewer

Length: 693 bp
ATGGCGGTTCCGGTGCTGGGTGGATTTCCGATCTTCATGGTAGTGAGAGTTTTGGGTTTCATCATCGCGGCTTTGGTTTTGACATGGACTGTTCATTACAGAGGAGGCTTAGCTCTTTCTTCTGATAACAAAGATCACATCTTTAATGTTCATCCAGTGATGATGGTGATTGGACTCATACTCTTCAATGGAGAAGCTATGTTAGCATATAAGTCAGTTCAAGGTACAAAGAACTTGAAAAAACTGGTTCATCTTACACTACAACTCACAGCATTTATTCTAAGCCTGATCGGAGTGTGGGCTGCTCTCAAGTTCCATATTGACAAAGGGATCGAAAATTTCTACAGTCTCCATTCGTGGCTCGGCCTTGCTTGCCTCTTTCTCTTTGCATTCCAGTGGGCTGCAGGGTTTGTGACTTATTGGTACCCGGGTGGTTCAAGAAACAGCAGAGCCAGTTTGATGCCATGGCATGTGTTCTTAGGGATTTCCATATATGCATTGGCGTTGGTGACTGCAACCACAGGGATACTTGAGAAGGTGACGTTCCTGCAAGTGAATCAAGTGATAACTCGGTATTCCACAGAGGCAATGTTAGTCAACACAATGGGCGTTCTAATCCTTATTCTTGGCGGTTTCGTGATACTCGGTGTGGTTACTCCGGTCAGTGGCAAGGATCAAGTTCTCACACAATAG

Protein Analysis

230

Amino Acids

25.29

Weight (kDa)

9.55

Isoelectric Point (pI)

32.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 50 - 182 9.7e-48 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38630
fragaria_vesca FvH4_6g45440 FvH4_6g45440 FvH4_6g45440
malus_domestica MD17G1073300.v1.1
prunus_persica Prupe.3G240600_v2.0.a1
pyrus_communis pycom17g07420
rosa_chinensis RchiOBHm_Chr2g0163271
rosa_laevigata RLG00000021387
rosa_roxburghii Rroxscaffold_2G00087420
rosa_rugosa Rorug02G0501900
rosa_samantha Rh2BG580800
rosa_wichuraiana Rw2G047050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 1 cut(s) 423
AccII CGCG 1 cut(s) 68
AciI CCGC 3 cut(s) 5, 68, 630
AclWI GGATC 2 cut(s) 338, 681
AcsI RAATTY 1 cut(s) 337
AfaI GTAC 2 cut(s) 226, 425
AfiI CCNNNNNNNGG 2 cut(s) 17, 665
AgsI TTSAA 4 cut(s) 187, 221, 238, 438
AluBI AGCT 2 cut(s) 113, 197
AluI AGCT 2 cut(s) 113, 197
Alw26I GTCTC 1 cut(s) 353
AlwI GGATC 2 cut(s) 338, 681
Ama87I CYCGRG 1 cut(s) 427
AoxI GGCC 1 cut(s) 364
ApeKI GCWGC 2 cut(s) 302, 401
ApoI RAATTY 1 cut(s) 337
Asp718I GGTACC 1 cut(s) 423
AsuC2I CCSGG 2 cut(s) 428, 429
AsuHPI GGTGA 3 cut(s) 178, 520, 550
AvaI CYCGRG 1 cut(s) 427
BaeI ACNNNNGTAYC 2 cut(s) 415, 448
BanI GGYRCC 1 cut(s) 423
BbvI GCAGC 2 cut(s) 289, 388
BccI CCATC 1 cut(s) 157
BciVI GTATCC 1 cut(s) 519
BcnI CCSGG 2 cut(s) 428, 429
BcoDI GTCTC 1 cut(s) 353
BfmI CTRYAG 2 cut(s) 343, 402
BfuI GTATCC 1 cut(s) 519
BisI GCNGC 3 cut(s) 69, 303, 402
BlpI GCTNAGC 1 cut(s) 109
BlsI GCNGC 3 cut(s) 70, 304, 403
Bme1390I CCNGG 2 cut(s) 428, 429
BmeT110I CYCGRG 1 cut(s) 427
BmiI GGNNCC 2 cut(s) 9, 425
BmrFI CCNGG 2 cut(s) 428, 429
BmsI GCATC 1 cut(s) 450
Bpu1102I GCTNAGC 1 cut(s) 109
BpuEI CTTGAG 2 cut(s) 293, 551
BpuMI CCSGG 2 cut(s) 428, 429
BsaJI CCNNGG 2 cut(s) 427, 464
BsaWI WCCGGW 2 cut(s) 10, 658
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 665
Bse1I ACTGG 4 cut(s) 155, 249, 394, 453
Bse3DI GCAATG 1 cut(s) 594
BseDI CCNNGG 2 cut(s) 427, 464
BseGI GGATG 1 cut(s) 151
BseLI CCNNNNNNNGG 2 cut(s) 17, 665
BseMI GCAATG 1 cut(s) 594
BseNI ACTGG 4 cut(s) 155, 249, 394, 453
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 2 cut(s) 289, 388
BseYI CCCAGC 1 cut(s) 16
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 1 cut(s) 366
BshNI GGYRCC 1 cut(s) 423
BsiHKCI CYCGRG 1 cut(s) 427
BsiSI CCGG 3 cut(s) 11, 428, 659
BslI CCNNNNNNNGG 2 cut(s) 17, 665
BsmAI GTCTC 1 cut(s) 353
BsmI GAATGC 1 cut(s) 389
BsnI GGCC 1 cut(s) 366
BsoBI CYCGRG 1 cut(s) 427
Bsp143I GATC 5 cut(s) 30, 133, 288, 330, 673
Bsp1720I GCTNAGC 1 cut(s) 109
Bsp19I CCATGG 1 cut(s) 464
BspACI CCGC 3 cut(s) 5, 68, 630
BspANI GGCC 1 cut(s) 366
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 2 cut(s) 9, 425
BspMAI CTGCAG 1 cut(s) 406
BspPI GGATC 2 cut(s) 338, 681
BspT107I GGYRCC 1 cut(s) 423
BsrDI GCAATG 1 cut(s) 594
BsrI ACTGG 4 cut(s) 155, 249, 394, 453
BssECI CCNNGG 2 cut(s) 427, 464
BssMI GATC 5 cut(s) 30, 133, 288, 330, 673
BssT1I CCWWGG 1 cut(s) 464
Bst4CI ACNGT 2 cut(s) 91, 347
Bst6I CTCTTC 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 373
BstDEI CTNAG 3 cut(s) 109, 281, 478
BstDSI CCRYGG 1 cut(s) 464
BstF5I GGATG 1 cut(s) 151
BstFNI CGCG 1 cut(s) 68
BstKTI GATC 5 cut(s) 33, 136, 291, 333, 676
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 5 cut(s) 30, 133, 288, 330, 673
BstMWI GCNNNNNNNGC 2 cut(s) 203, 372
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 426, 427
BstSFI CTRYAG 2 cut(s) 343, 402
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 289, 388
BsuI GTATCC 1 cut(s) 519
BsuRI GGCC 1 cut(s) 366
BtgI CCRYGG 1 cut(s) 464
BtgZI GCGATG 1 cut(s) 49
BtsCI GGATG 1 cut(s) 151
BtsIMutI CAGTG 3 cut(s) 162, 401, 670
Cac8I GCNNGC 1 cut(s) 373
Cfr9I CCCGGG 1 cut(s) 427
Csp6I GTAC 2 cut(s) 225, 424
CviAII CATG 4 cut(s) 37, 84, 465, 470
CviQI GTAC 2 cut(s) 225, 424
DdeI CTNAG 3 cut(s) 109, 281, 478
DpnI GATC 5 cut(s) 32, 135, 290, 332, 675
DpnII GATC 5 cut(s) 30, 133, 288, 330, 673
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
Eco130I CCWWGG 1 cut(s) 464
Eco88I CYCGRG 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 464
EcoT22I ATGCAT 1 cut(s) 499
ErhI CCWWGG 1 cut(s) 464
FaeI CATG 4 cut(s) 40, 87, 468, 473
FatI CATG 4 cut(s) 36, 83, 464, 469
Fnu4HI GCNGC 3 cut(s) 69, 303, 402
FokI GGATG 1 cut(s) 138
Fsp4HI GCNGC 3 cut(s) 69, 303, 402
GluI GCNGC 3 cut(s) 69, 303, 402
GsaI CCCAGC 1 cut(s) 20
HaeIII GGCC 1 cut(s) 366
HapII CCGG 3 cut(s) 11, 428, 659
Hin1II CATG 4 cut(s) 40, 87, 468, 473
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 2 cut(s) 174, 556
HpaII CCGG 3 cut(s) 11, 428, 659
HphI GGTGA 3 cut(s) 178, 520, 550
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 3 cut(s) 30, 124, 293
Hpy188III TCNNGA 2 cut(s) 438, 637
Hpy8I GTNNAC 1 cut(s) 598
HpyAV CCTTC 1 cut(s) 529
HpyCH4III ACNGT 2 cut(s) 91, 347
HpyCH4IV ACGT 1 cut(s) 542
HpyCH4V TGCA 5 cut(s) 389, 404, 497, 515, 550
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 372
HpyF3I CTNAG 3 cut(s) 109, 281, 478
HpySE526I ACGT 1 cut(s) 542
Hsp92II CATG 4 cut(s) 40, 87, 468, 473
KpnI GGTACC 1 cut(s) 427
Kzo9I GATC 5 cut(s) 30, 133, 288, 330, 673
Lsp1109I GCAGC 2 cut(s) 289, 388
LweI GCATC 1 cut(s) 450
MaeII ACGT 1 cut(s) 542
MaeIII GTNAC 4 cut(s) 412, 508, 538, 652
MalI GATC 5 cut(s) 32, 135, 290, 332, 675
MboI GATC 5 cut(s) 30, 133, 288, 330, 673
MboII GAAGA 3 cut(s) 25, 111, 175
MluCI AATT 1 cut(s) 337
MlyI GAGTC 1 cut(s) 168
MnlI CCTC 4 cut(s) 95, 98, 386, 577
Mph1103I ATGCAT 1 cut(s) 499
MseI TTAA 1 cut(s) 144
MslI CAYNNNNRTG 2 cut(s) 41, 394
MspI CCGG 3 cut(s) 11, 428, 659
MspR9I CCNGG 2 cut(s) 428, 429
Mva1269I GAATGC 1 cut(s) 389
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 2 cut(s) 203, 372
NciI CCSGG 2 cut(s) 428, 429
NcoI CCATGG 1 cut(s) 464
NdeII GATC 5 cut(s) 30, 133, 288, 330, 673
NlaIII CATG 4 cut(s) 40, 87, 468, 473
NlaIV GGNNCC 2 cut(s) 9, 425
NmeAIII GCCGAG 1 cut(s) 342
NmuCI GTSAC 3 cut(s) 412, 508, 538
NsiI ATGCAT 1 cut(s) 499
NspI RCATGY 1 cut(s) 473
PctI GAATGC 1 cut(s) 389
PfeI GAWTC 1 cut(s) 556
PkrI GCNGC 3 cut(s) 70, 304, 403
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
PspFI CCCAGC 1 cut(s) 16
PspN4I GGNNCC 2 cut(s) 9, 425
PstI CTGCAG 1 cut(s) 406
RsaI GTAC 2 cut(s) 226, 425
RsaNI GTAC 2 cut(s) 225, 424
RseI CAYNNNNRTG 2 cut(s) 41, 394
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 3 cut(s) 69, 303, 402
Sau3AI GATC 5 cut(s) 30, 133, 288, 330, 673
SchI GAGTC 1 cut(s) 168
ScrFI CCNGG 2 cut(s) 428, 429
SetI ASST 5 cut(s) 115, 199, 226, 540, 545
SfaNI GCATC 1 cut(s) 450
SfcI CTRYAG 2 cut(s) 343, 402
SmaI CCCGGG 1 cut(s) 429
SmiMI CAYNNNNRTG 2 cut(s) 41, 394
SmlI CTYRAG 2 cut(s) 308, 530
SmoI CTYRAG 2 cut(s) 308, 530
Sse9I AATT 1 cut(s) 337
SsiI CCGC 3 cut(s) 5, 68, 630
StyD4I CCNGG 2 cut(s) 426, 427
StyI CCWWGG 1 cut(s) 464
TaaI ACNGT 2 cut(s) 91, 347
TaiI ACGT 1 cut(s) 545
TaqI TCGA 1 cut(s) 333
TasI AATT 1 cut(s) 337
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 1 cut(s) 556
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 3 cut(s) 162, 401, 670
TseFI GTSAC 3 cut(s) 412, 508, 538
TseI GCWGC 2 cut(s) 302, 401
Tsp45I GTSAC 3 cut(s) 412, 508, 538
TspDTI ATGAA 5 cut(s) 25, 49, 83, 140, 239
TspMI CCCGGG 1 cut(s) 427
TspRI CASTG 3 cut(s) 162, 401, 670
XapI RAATTY 1 cut(s) 337
XceI RCATGY 1 cut(s) 473
XcmI CCANNNNNNNNNTGG 1 cut(s) 496
XmaI CCCGGG 1 cut(s) 427
Zsp2I ATGCAT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.