FvH4_6g45440

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017157
Physical Location & Seq
Reverse (-)
34882264 .. 34884084
1821 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45440.t2

Sequence Viewer

Length: 699 bp
ATGGGGGCGATTCCGGTAGTGCGGTTCCCGATCTTCCGAGCAGTGCGAGTTGTCGGAGTGGCAGTGGCCGCTCTAGTCCTCACCTGGACCATACATTTCCGGGGAGGCTTGGCTCTCATCTCTGACAACAAAGATCTCATCTTCAATGTTCATCCAGTGTTGATGGTGATTGGACTTGTAATCTTGAATGGTGAAGCCATGCTAGCGTACAAGACAGTATCAGGAACAAAGAGCTTCAAGAAATTAGTTCACCTTACGCTGCAATTCATTGCGTTTTGTTTAAGCATTGTGGGTGTGTGGGCTGCCTTGAAGTTCCACAATGATAAGGGCATTGACAATTTCTACAGCCTGCATTCTTGGCTGGGTCTAGCCTGTGTGTTCCTTTTTACCATCCAGGGGGCTGCTGGATTTGTTACATATTGGTACCCAGGTGGCTCGAAAAATAGTAGAGCAAACTTGATGCCATGGCATGTGTTTTTCGGTGTTTATATATATGCACTTGCTGTTGTTACTGTGACTACTGGTATCTTAGAAAAAGCCACATTTCTTCAAGCCAACCATGTAATATCACGCTACTCAACTGAAGCTCTGCTGATAAATTCTCTGGGGATATTGGTTGTTGCACTGGGCGGTTTTGTTATTCTCGCGGTCATTGCTCCTGCTAATAAAGGTGACATTTTCAGAGAATCAACAGAGTAA

Protein Analysis

233

Amino Acids

25.29

Weight (kDa)

9.55

Isoelectric Point (pI)

25.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 50 - 182 1.1e-47 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38630
fragaria_vesca FvH4_6g45440 FvH4_6g45440 FvH4_6g45440
malus_domestica MD17G1073300.v1.1
prunus_persica Prupe.3G240600_v2.0.a1
pyrus_communis pycom17g07420
rosa_chinensis RchiOBHm_Chr2g0163271
rosa_laevigata RLG00000021387
rosa_roxburghii Rroxscaffold_2G00087420
rosa_rugosa Rorug02G0501900
rosa_samantha Rh2BG580800
rosa_wichuraiana Rw2G047050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 1 cut(s) 423
AccBSI CCGCTC 1 cut(s) 71
AccII CGCG 1 cut(s) 647
AciI CCGC 4 cut(s) 22, 69, 630, 647
AcoI YGGCCR 1 cut(s) 66
AcsI RAATTY 1 cut(s) 598
AcuI CTGAAG 1 cut(s) 603
AfaI GTAC 2 cut(s) 209, 425
AfiI CCNNNNNNNGG 1 cut(s) 396
AgsI TTSAA 5 cut(s) 145, 187, 238, 310, 551
AjnI CCWGG 3 cut(s) 83, 393, 427
AluBI AGCT 2 cut(s) 234, 587
AluI AGCT 2 cut(s) 234, 587
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 3 cut(s) 259, 302, 401
ApoI RAATTY 1 cut(s) 598
Asp718I GGTACC 1 cut(s) 423
AspS9I GGNCC 1 cut(s) 87
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 5 cut(s) 73, 178, 203, 242, 683
AsuNHI GCTAGC 1 cut(s) 202
AvaII GGWCC 1 cut(s) 87
BaeI ACNNNNGTAYC 2 cut(s) 415, 448
BanI GGYRCC 1 cut(s) 423
BbvI GCAGC 3 cut(s) 246, 289, 388
BccI CCATC 2 cut(s) 157, 398
BciT130I CCWGG 3 cut(s) 85, 395, 429
BcnI CCSGG 1 cut(s) 101
BfaI CTAG 3 cut(s) 74, 203, 368
BfmI CTRYAG 1 cut(s) 343
BglII AGATCT 1 cut(s) 133
BisI GCNGC 4 cut(s) 69, 260, 303, 402
BlsI GCNGC 4 cut(s) 70, 261, 304, 403
Bme1390I CCNGG 4 cut(s) 85, 101, 395, 429
Bme18I GGWCC 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 2 cut(s) 26, 425
BmrFI CCNGG 4 cut(s) 85, 101, 395, 429
BmrI ACTGGG 1 cut(s) 635
BmsI GCATC 1 cut(s) 450
BmtI GCTAGC 1 cut(s) 206
BmuI ACTGGG 1 cut(s) 635
BpuMI CCSGG 1 cut(s) 101
BsaJI CCNNGG 4 cut(s) 100, 394, 427, 464
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse1I ACTGG 3 cut(s) 155, 526, 630
Bse3DI GCAATG 2 cut(s) 267, 651
BseBI CCWGG 3 cut(s) 85, 395, 429
BseDI CCNNGG 4 cut(s) 100, 394, 427, 464
BseGI GGATG 2 cut(s) 151, 390
BseLI CCNNNNNNNGG 1 cut(s) 396
BseMI GCAATG 2 cut(s) 267, 651
BseNI ACTGG 3 cut(s) 155, 526, 630
BseXI GCAGC 3 cut(s) 246, 289, 388
BseYI CCCAGC 1 cut(s) 361
Bsh1236I CGCG 1 cut(s) 647
BshFI GGCC 1 cut(s) 68
BshNI GGYRCC 1 cut(s) 423
BsiSI CCGG 2 cut(s) 14, 100
BslI CCNNNNNNNGG 1 cut(s) 396
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 1 cut(s) 68
Bsp143I GATC 2 cut(s) 30, 133
Bsp19I CCATGG 1 cut(s) 464
BspACI CCGC 4 cut(s) 22, 69, 630, 647
BspANI GGCC 1 cut(s) 68
BspFNI CGCG 1 cut(s) 647
BspLI GGNNCC 2 cut(s) 26, 425
BspOI GCTAGC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 423
BsrBI CCGCTC 1 cut(s) 71
BsrDI GCAATG 2 cut(s) 267, 651
BsrI ACTGG 3 cut(s) 155, 526, 630
BssECI CCNNGG 4 cut(s) 100, 394, 427, 464
BssMI GATC 2 cut(s) 30, 133
BssT1I CCWWGG 1 cut(s) 464
Bst2UI CCWGG 3 cut(s) 85, 395, 429
Bst4CI ACNGT 2 cut(s) 217, 514
BstC8I GCNNGC 2 cut(s) 204, 350
BstDEI CTNAG 1 cut(s) 529
BstDSI CCRYGG 1 cut(s) 464
BstF5I GGATG 2 cut(s) 151, 390
BstFNI CGCG 1 cut(s) 647
BstKTI GATC 2 cut(s) 33, 136
BstMBI GATC 2 cut(s) 30, 133
BstMWI GCNNNNNNNGC 4 cut(s) 68, 203, 358, 653
BstNI CCWGG 3 cut(s) 85, 395, 429
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 4 cut(s) 83, 99, 393, 427
BstSFI CTRYAG 1 cut(s) 343
BstUI CGCG 1 cut(s) 647
BstV1I GCAGC 3 cut(s) 246, 289, 388
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuRI GGCC 1 cut(s) 68
BtgI CCRYGG 1 cut(s) 464
BtsCI GGATG 2 cut(s) 151, 390
BtsI GCAGTG 2 cut(s) 48, 69
BtsIMutI CAGTG 4 cut(s) 48, 69, 162, 623
Cac8I GCNNGC 2 cut(s) 204, 350
Cfr13I GGNCC 1 cut(s) 87
Csp6I GTAC 2 cut(s) 208, 424
CviAII CATG 4 cut(s) 199, 465, 470, 560
CviQI GTAC 2 cut(s) 208, 424
DdeI CTNAG 1 cut(s) 529
DpnI GATC 2 cut(s) 32, 135
DpnII GATC 2 cut(s) 30, 133
EaeI YGGCCR 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 464
Eco47I GGWCC 1 cut(s) 87
Eco57I CTGAAG 1 cut(s) 603
EcoRII CCWGG 3 cut(s) 83, 393, 427
EcoT14I CCWWGG 1 cut(s) 464
ErhI CCWWGG 1 cut(s) 464
FaeI CATG 4 cut(s) 202, 468, 473, 563
FatI CATG 4 cut(s) 198, 464, 469, 559
Fnu4HI GCNGC 4 cut(s) 69, 260, 303, 402
FokI GGATG 2 cut(s) 138, 377
Fsp4HI GCNGC 4 cut(s) 69, 260, 303, 402
FspBI CTAG 3 cut(s) 74, 203, 368
GluI GCNGC 4 cut(s) 69, 260, 303, 402
GsaI CCCAGC 1 cut(s) 365
HaeIII GGCC 1 cut(s) 68
HapII CCGG 2 cut(s) 14, 100
Hin1II CATG 4 cut(s) 202, 468, 473, 563
HinfI GANTC 2 cut(s) 10, 686
HpaII CCGG 2 cut(s) 14, 100
HphI GGTGA 5 cut(s) 73, 178, 203, 242, 683
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 4 cut(s) 38, 56, 124, 683
Hpy188III TCNNGA 4 cut(s) 28, 184, 222, 238
Hpy8I GTNNAC 1 cut(s) 250
HpyCH4III ACNGT 2 cut(s) 217, 514
HpyCH4V TGCA 4 cut(s) 262, 352, 497, 623
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 203, 358, 653
HpyF3I CTNAG 1 cut(s) 529
Hsp92II CATG 4 cut(s) 202, 468, 473, 563
KpnI GGTACC 1 cut(s) 427
Kzo9I GATC 2 cut(s) 30, 133
LmnI GCTCC 1 cut(s) 661
Lsp1109I GCAGC 3 cut(s) 246, 289, 388
LweI GCATC 1 cut(s) 450
MaeI CTAG 3 cut(s) 74, 203, 368
MaeIII GTNAC 4 cut(s) 412, 508, 514, 671
MalI GATC 2 cut(s) 32, 135
MbiI CCGCTC 1 cut(s) 71
MboI GATC 2 cut(s) 30, 133
MboII GAAGA 3 cut(s) 25, 133, 539
MflI RGATCY 1 cut(s) 133
MluCI AATT 4 cut(s) 242, 263, 337, 598
MmeI TCCRAC 1 cut(s) 34
MnlI CCTC 2 cut(s) 89, 98
MseI TTAA 1 cut(s) 281
MspI CCGG 2 cut(s) 14, 100
MspR9I CCNGG 4 cut(s) 85, 101, 395, 429
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 3 cut(s) 85, 395, 429
MvnI CGCG 1 cut(s) 647
MwoI GCNNNNNNNGC 4 cut(s) 68, 203, 358, 653
NciI CCSGG 1 cut(s) 101
NcoI CCATGG 1 cut(s) 464
NdeII GATC 2 cut(s) 30, 133
NheI GCTAGC 1 cut(s) 202
NlaIII CATG 4 cut(s) 202, 468, 473, 563
NlaIV GGNNCC 2 cut(s) 26, 425
NmuCI GTSAC 2 cut(s) 514, 671
NspI RCATGY 1 cut(s) 473
PctI GAATGC 1 cut(s) 352
PfeI GAWTC 2 cut(s) 10, 686
PkrI GCNGC 4 cut(s) 70, 261, 304, 403
Psp6I CCWGG 3 cut(s) 83, 393, 427
PspFI CCCAGC 1 cut(s) 361
PspGI CCWGG 3 cut(s) 83, 393, 427
PspN4I GGNNCC 2 cut(s) 26, 425
PspPI GGNCC 1 cut(s) 87
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 2 cut(s) 209, 425
RsaNI GTAC 2 cut(s) 208, 424
SaqAI TTAA 1 cut(s) 281
SatI GCNGC 4 cut(s) 69, 260, 303, 402
Sau3AI GATC 2 cut(s) 30, 133
Sau96I GGNCC 1 cut(s) 87
ScrFI CCNGG 4 cut(s) 85, 101, 395, 429
SetI ASST 6 cut(s) 86, 236, 255, 433, 589, 673
SfaNI GCATC 1 cut(s) 450
SfcI CTRYAG 1 cut(s) 343
SinI GGWCC 1 cut(s) 87
Sse9I AATT 4 cut(s) 242, 263, 337, 598
SsiI CCGC 4 cut(s) 22, 69, 630, 647
SspMI CTAG 3 cut(s) 74, 203, 368
StyD4I CCNGG 4 cut(s) 83, 99, 393, 427
StyI CCWWGG 1 cut(s) 464
TaaI ACNGT 2 cut(s) 217, 514
TaqI TCGA 1 cut(s) 437
TasI AATT 4 cut(s) 242, 263, 337, 598
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 2 cut(s) 10, 686
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TscAI CASTG 4 cut(s) 48, 69, 162, 630
TseFI GTSAC 2 cut(s) 514, 671
TseI GCWGC 3 cut(s) 259, 302, 401
Tsp45I GTSAC 2 cut(s) 514, 671
TspDTI ATGAA 2 cut(s) 140, 256
TspRI CASTG 4 cut(s) 48, 69, 162, 630
VpaK11BI GGWCC 1 cut(s) 87
XapI RAATTY 1 cut(s) 598
XceI RCATGY 1 cut(s) 473
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XspI CTAG 3 cut(s) 74, 203, 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.