Rh2BG580800

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0017157
Physical Location & Seq
Reverse (-)
79742718 .. 79744388
1671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG580800.1

Sequence Viewer

Length: 699 bp
ATGGGGGCGATTCCAGTAGTCCGGTTCCCGATCTTCCGGGCAGTGAGAGTGATCGGTGTGGCAGTGGCGGCTCTGGTCCTGACCTGGACCATGCATTTCCGAGGAGGCTTGGCTCTCGTCTCTGACAACAAAGATCTCATCTTCAATGTTCATCCAGTGTTGATGGTGATTGGACTCGTAATCTTAAATGGTGAAGCCATGCTAGCATACAAGACGATGCCAGGAACAAAAAGCTTCAAGAAATTAGTTCACCTTACACTACAATTCCTTGCTTTTTGTTTAAGCATTGTCGGTGTATGGGCTGCTTTGAAGTTCCACAATGATAAGGGCATTGACAATTTCTACAGTCTGCATTCTTGGCTGGGTCTAGCTTGCCTGTTCCTTTTTACCATCCAGTGGGCTGCTGGATTTGTTACATATTGGTACCCAGGTGGCTCGAAAAATAGTAGAGCTAGCTTGCTGCCATGGCATGTCTTTTTCGGTGTTTATATATATGCACTCGCTGTTGTTACTGTAACTACTGGTATCCTAGAAAAAGCCACATTTCTTCAAGCCAACCATGTAATATCACGTTATTCAACCGAAGCTTTGCTGATAAATTCTCTGGGATTATTGGTCGTTGCGCTGGGCGGTTTTGTTATTCTCGCGGTTATTGCTCCTGCTAATAAAGGTGACATTTTCAGAGAATCAATAGAGTAA

Protein Analysis

232

Amino Acids

25.46

Weight (kDa)

9.55

Isoelectric Point (pI)

30.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 50 - 182 6.8e-49 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38630
fragaria_vesca FvH4_6g45440 FvH4_6g45440 FvH4_6g45440
malus_domestica MD17G1073300.v1.1
prunus_persica Prupe.3G240600_v2.0.a1
pyrus_communis pycom17g07420
rosa_chinensis RchiOBHm_Chr2g0163271
rosa_laevigata RLG00000021387
rosa_roxburghii Rroxscaffold_2G00087420
rosa_rugosa Rorug02G0501900
rosa_samantha Rh2BG580800
rosa_wichuraiana Rw2G047050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 1 cut(s) 423
AccB7I CCANNNNNTGG 1 cut(s) 396
AccII CGCG 1 cut(s) 647
AciI CCGC 3 cut(s) 68, 630, 647
AcsI RAATTY 1 cut(s) 598
AfaI GTAC 1 cut(s) 425
AfiI CCNNNNNNNGG 1 cut(s) 396
AgsI TTSAA 5 cut(s) 145, 238, 310, 551, 579
AjnI CCWGG 3 cut(s) 83, 220, 427
AluBI AGCT 5 cut(s) 234, 371, 452, 456, 587
AluI AGCT 5 cut(s) 234, 371, 452, 456, 587
Alw26I GTCTC 1 cut(s) 124
ApeKI GCWGC 3 cut(s) 302, 401, 460
ApoI RAATTY 1 cut(s) 598
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 1 cut(s) 625
AspS9I GGNCC 2 cut(s) 76, 87
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 4 cut(s) 178, 203, 242, 683
AsuNHI GCTAGC 2 cut(s) 202, 452
AvaII GGWCC 2 cut(s) 76, 87
BaeI ACNNNNGTAYC 2 cut(s) 415, 448
BanI GGYRCC 1 cut(s) 423
BbvI GCAGC 3 cut(s) 289, 388, 447
BccI CCATC 2 cut(s) 157, 398
BciT130I CCWGG 3 cut(s) 85, 222, 429
BciVI GTATCC 1 cut(s) 536
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 124
BfaI CTAG 4 cut(s) 203, 368, 453, 530
BfmI CTRYAG 1 cut(s) 343
BfuI GTATCC 1 cut(s) 536
BglII AGATCT 1 cut(s) 133
BisI GCNGC 4 cut(s) 69, 303, 402, 461
BlsI GCNGC 4 cut(s) 70, 304, 403, 462
Bme1390I CCNGG 4 cut(s) 38, 85, 222, 429
Bme18I GGWCC 2 cut(s) 76, 87
BmgT120I GGNCC 2 cut(s) 76, 87
BmiI GGNNCC 2 cut(s) 26, 425
BmrFI CCNGG 4 cut(s) 38, 85, 222, 429
BmsI GCATC 1 cut(s) 207
BmtI GCTAGC 2 cut(s) 206, 456
BpuMI CCSGG 1 cut(s) 38
BsaJI CCNNGG 3 cut(s) 100, 427, 464
BsaWI WCCGGW 1 cut(s) 21
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse1I ACTGG 4 cut(s) 14, 155, 394, 526
BseBI CCWGG 3 cut(s) 85, 222, 429
BseDI CCNNGG 3 cut(s) 100, 427, 464
BseGI GGATG 2 cut(s) 151, 390
BseLI CCNNNNNNNGG 1 cut(s) 396
BseNI ACTGG 4 cut(s) 14, 155, 394, 526
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 3 cut(s) 289, 388, 447
BseYI CCCAGC 2 cut(s) 361, 625
Bsh1236I CGCG 1 cut(s) 647
BshNI GGYRCC 1 cut(s) 423
BsiSI CCGG 2 cut(s) 22, 37
BslI CCNNNNNNNGG 1 cut(s) 396
BsmAI GTCTC 1 cut(s) 124
BsmBI CGTCTC 1 cut(s) 124
BsmI GAATGC 1 cut(s) 352
Bsp143I GATC 3 cut(s) 30, 51, 133
Bsp19I CCATGG 1 cut(s) 464
BspACI CCGC 3 cut(s) 68, 630, 647
BspFNI CGCG 1 cut(s) 647
BspLI GGNNCC 2 cut(s) 26, 425
BspOI GCTAGC 2 cut(s) 206, 456
BspT107I GGYRCC 1 cut(s) 423
BsrI ACTGG 4 cut(s) 14, 155, 394, 526
BssECI CCNNGG 3 cut(s) 100, 427, 464
BssMI GATC 3 cut(s) 30, 51, 133
BssT1I CCWWGG 1 cut(s) 464
Bst2UI CCWGG 3 cut(s) 85, 222, 429
Bst4CI ACNGT 2 cut(s) 347, 514
BstC8I GCNNGC 4 cut(s) 204, 373, 454, 458
BstDSI CCRYGG 1 cut(s) 464
BstF5I GGATG 2 cut(s) 151, 390
BstFNI CGCG 1 cut(s) 647
BstHHI GCGC 1 cut(s) 625
BstKTI GATC 3 cut(s) 33, 54, 136
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 3 cut(s) 30, 51, 133
BstMWI GCNNNNNNNGC 5 cut(s) 68, 203, 358, 466, 653
BstNI CCWGG 3 cut(s) 85, 222, 429
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 4 cut(s) 36, 83, 220, 427
BstSFI CTRYAG 1 cut(s) 343
BstUI CGCG 1 cut(s) 647
BstV1I GCAGC 3 cut(s) 289, 388, 447
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuI GTATCC 1 cut(s) 536
BtgI CCRYGG 1 cut(s) 464
BtsCI GGATG 2 cut(s) 151, 390
BtsI GCAGTG 2 cut(s) 48, 69
BtsIMutI CAGTG 4 cut(s) 48, 69, 162, 401
Cac8I GCNNGC 4 cut(s) 204, 373, 454, 458
CfoI GCGC 1 cut(s) 625
Cfr13I GGNCC 2 cut(s) 76, 87
Csp6I GTAC 1 cut(s) 424
CviAII CATG 5 cut(s) 91, 199, 465, 470, 560
CviQI GTAC 1 cut(s) 424
DpnI GATC 3 cut(s) 32, 53, 135
DpnII GATC 3 cut(s) 30, 51, 133
Eco130I CCWWGG 1 cut(s) 464
Eco47I GGWCC 2 cut(s) 76, 87
EcoRII CCWGG 3 cut(s) 83, 220, 427
EcoT14I CCWWGG 1 cut(s) 464
EcoT22I ATGCAT 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 464
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 5 cut(s) 94, 202, 468, 473, 563
FatI CATG 5 cut(s) 90, 198, 464, 469, 559
Fnu4HI GCNGC 4 cut(s) 69, 303, 402, 461
FokI GGATG 2 cut(s) 138, 377
Fsp4HI GCNGC 4 cut(s) 69, 303, 402, 461
FspBI CTAG 4 cut(s) 203, 368, 453, 530
GlaI GCGC 1 cut(s) 624
GluI GCNGC 4 cut(s) 69, 303, 402, 461
GsaI CCCAGC 2 cut(s) 365, 629
HapII CCGG 2 cut(s) 22, 37
HhaI GCGC 1 cut(s) 625
Hin1II CATG 5 cut(s) 94, 202, 468, 473, 563
Hin6I GCGC 1 cut(s) 623
HinP1I GCGC 1 cut(s) 623
HindIII AAGCTT 2 cut(s) 232, 585
HinfI GANTC 3 cut(s) 10, 174, 686
HpaII CCGG 2 cut(s) 22, 37
HphI GGTGA 4 cut(s) 178, 203, 242, 683
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 3 cut(s) 101, 124, 683
Hpy188III TCNNGA 3 cut(s) 28, 79, 238
Hpy8I GTNNAC 1 cut(s) 250
HpyCH4III ACNGT 2 cut(s) 347, 514
HpyCH4IV ACGT 1 cut(s) 571
HpyCH4V TGCA 3 cut(s) 94, 352, 497
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 203, 358, 466, 653
HpySE526I ACGT 1 cut(s) 571
Hsp92II CATG 5 cut(s) 94, 202, 468, 473, 563
HspAI GCGC 1 cut(s) 623
KpnI GGTACC 1 cut(s) 427
Kzo9I GATC 3 cut(s) 30, 51, 133
LmnI GCTCC 1 cut(s) 661
Lsp1109I GCAGC 3 cut(s) 289, 388, 447
LweI GCATC 1 cut(s) 207
MaeI CTAG 4 cut(s) 203, 368, 453, 530
MaeII ACGT 1 cut(s) 571
MaeIII GTNAC 4 cut(s) 412, 508, 514, 671
MalI GATC 3 cut(s) 32, 53, 135
MboI GATC 3 cut(s) 30, 51, 133
MboII GAAGA 3 cut(s) 25, 133, 539
MflI RGATCY 1 cut(s) 133
MluCI AATT 4 cut(s) 242, 263, 337, 598
MlyI GAGTC 1 cut(s) 168
MnlI CCTC 2 cut(s) 95, 98
Mph1103I ATGCAT 1 cut(s) 96
MseI TTAA 2 cut(s) 185, 281
MspI CCGG 2 cut(s) 22, 37
MspR9I CCNGG 4 cut(s) 38, 85, 222, 429
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 3 cut(s) 85, 222, 429
MvnI CGCG 1 cut(s) 647
MwoI GCNNNNNNNGC 5 cut(s) 68, 203, 358, 466, 653
NciI CCSGG 1 cut(s) 38
NcoI CCATGG 1 cut(s) 464
NdeII GATC 3 cut(s) 30, 51, 133
NheI GCTAGC 2 cut(s) 202, 452
NlaIII CATG 5 cut(s) 94, 202, 468, 473, 563
NlaIV GGNNCC 2 cut(s) 26, 425
NmuCI GTSAC 1 cut(s) 671
NsiI ATGCAT 1 cut(s) 96
NspI RCATGY 1 cut(s) 473
PctI GAATGC 1 cut(s) 352
PfeI GAWTC 2 cut(s) 10, 686
PflMI CCANNNNNTGG 1 cut(s) 396
PkrI GCNGC 4 cut(s) 70, 304, 403, 462
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
Psp6I CCWGG 3 cut(s) 83, 220, 427
PspFI CCCAGC 2 cut(s) 361, 625
PspGI CCWGG 3 cut(s) 83, 220, 427
PspN4I GGNNCC 2 cut(s) 26, 425
PspPI GGNCC 2 cut(s) 76, 87
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
SaqAI TTAA 2 cut(s) 185, 281
SatI GCNGC 4 cut(s) 69, 303, 402, 461
Sau3AI GATC 3 cut(s) 30, 51, 133
Sau96I GGNCC 2 cut(s) 76, 87
SchI GAGTC 1 cut(s) 168
ScrFI CCNGG 4 cut(s) 38, 85, 222, 429
SfaNI GCATC 1 cut(s) 207
SfcI CTRYAG 1 cut(s) 343
SinI GGWCC 2 cut(s) 76, 87
Sse9I AATT 4 cut(s) 242, 263, 337, 598
SsiI CCGC 3 cut(s) 68, 630, 647
SspMI CTAG 4 cut(s) 203, 368, 453, 530
StyD4I CCNGG 4 cut(s) 36, 83, 220, 427
StyI CCWWGG 1 cut(s) 464
TaaI ACNGT 2 cut(s) 347, 514
TaiI ACGT 1 cut(s) 574
TaqI TCGA 1 cut(s) 437
TasI AATT 4 cut(s) 242, 263, 337, 598
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 2 cut(s) 10, 686
Tru1I TTAA 2 cut(s) 185, 281
Tru9I TTAA 2 cut(s) 185, 281
TscAI CASTG 4 cut(s) 48, 69, 162, 401
TseFI GTSAC 1 cut(s) 671
TseI GCWGC 3 cut(s) 302, 401, 460
Tsp45I GTSAC 1 cut(s) 671
TspDTI ATGAA 1 cut(s) 140
TspRI CASTG 4 cut(s) 48, 69, 162, 401
Van91I CCANNNNNTGG 1 cut(s) 396
VpaK11BI GGWCC 2 cut(s) 76, 87
XapI RAATTY 1 cut(s) 598
XceI RCATGY 1 cut(s) 473
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XspI CTAG 4 cut(s) 203, 368, 453, 530
Zsp2I ATGCAT 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.