Rw2G047050

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017157
Physical Location & Seq
Reverse (-)
74456022 .. 74457913
1892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G047050.1

Sequence Viewer

Length: 699 bp
ATGGGGGCGATTCCAGTAGTCCGGTTCCCGATCTTCCGGGCAGTGAGAGTGATCGGAGTGGCAGTGGCGGCTCTGGTCCTGACCTGGACCATGCATTTCCGAGGAGGCTTGGCTCTCGTCTCTGACAACAAAGATCTCATCTTCAATGTTCATCCAGTGTTGATGGTGATTGGACTCGTAATCTTAAATGGTGAAGCCATGCTAGCATACAAGACGATGCCAGGAACAAAAAGCTTCAAGAAATTAGTTCACCTTACACTACAATTCCTTGCTTTTTGTTTAAGCATTGTCGGTGTATGGGCTGCTTTGAAGTTCCACAATGATAAGGGCATTGACAATTTCTACAGTCTGCATTCTTGGCTGGGTCTAGCTTGCCTGTTCCTTTTTACCATCCAGTGGGCTGCTGGATTTGTTACATATTGGTACCCAGGTGGCTCGAAAAATAGTAGAGCTAGCTTGCTGCCATGGCATGTCTTTTTCGGTGTTTATATATATGCACTCGCTGTTGTTACTGTAACTACTGGTATCCTAGAAAAAGCCACATTTCTTCAAGCCAACCATGTAATATCACGTTATTCAACAGAAGCTTTGCTGATAAATTCTCTGGGATTATTGGTCGTTGCGCTGGGCGGTTTTGTTATTCTCGCGGTTATTGCTCCTGCTAATAAAGGTGACATTTTCAGAGAATCAATAGAGTAA

Protein Analysis

232

Amino Acids

25.46

Weight (kDa)

9.55

Isoelectric Point (pI)

30.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 50 - 182 6.8e-49 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38630
fragaria_vesca FvH4_6g45440 FvH4_6g45440 FvH4_6g45440
malus_domestica MD17G1073300.v1.1
prunus_persica Prupe.3G240600_v2.0.a1
pyrus_communis pycom17g07420
rosa_chinensis RchiOBHm_Chr2g0163271
rosa_laevigata RLG00000021387
rosa_roxburghii Rroxscaffold_2G00087420
rosa_rugosa Rorug02G0501900
rosa_samantha Rh2BG580800
rosa_wichuraiana Rw2G047050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 1 cut(s) 423
AccB7I CCANNNNNTGG 1 cut(s) 396
AccII CGCG 1 cut(s) 647
AciI CCGC 3 cut(s) 68, 630, 647
AcsI RAATTY 1 cut(s) 598
AfaI GTAC 1 cut(s) 425
AfiI CCNNNNNNNGG 1 cut(s) 396
AgsI TTSAA 5 cut(s) 145, 238, 310, 551, 579
AjnI CCWGG 3 cut(s) 83, 220, 427
AluBI AGCT 5 cut(s) 234, 371, 452, 456, 587
AluI AGCT 5 cut(s) 234, 371, 452, 456, 587
Alw26I GTCTC 1 cut(s) 124
ApeKI GCWGC 3 cut(s) 302, 401, 460
ApoI RAATTY 1 cut(s) 598
Asp718I GGTACC 1 cut(s) 423
AspLEI GCGC 1 cut(s) 625
AspS9I GGNCC 2 cut(s) 76, 87
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 4 cut(s) 178, 203, 242, 683
AsuNHI GCTAGC 2 cut(s) 202, 452
AvaII GGWCC 2 cut(s) 76, 87
BaeI ACNNNNGTAYC 2 cut(s) 415, 448
BanI GGYRCC 1 cut(s) 423
BbvI GCAGC 3 cut(s) 289, 388, 447
BccI CCATC 2 cut(s) 157, 398
BciT130I CCWGG 3 cut(s) 85, 222, 429
BciVI GTATCC 1 cut(s) 536
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 124
BfaI CTAG 4 cut(s) 203, 368, 453, 530
BfmI CTRYAG 1 cut(s) 343
BfuI GTATCC 1 cut(s) 536
BglII AGATCT 1 cut(s) 133
BisI GCNGC 4 cut(s) 69, 303, 402, 461
BlsI GCNGC 4 cut(s) 70, 304, 403, 462
Bme1390I CCNGG 4 cut(s) 38, 85, 222, 429
Bme18I GGWCC 2 cut(s) 76, 87
BmgT120I GGNCC 2 cut(s) 76, 87
BmiI GGNNCC 2 cut(s) 26, 425
BmrFI CCNGG 4 cut(s) 38, 85, 222, 429
BmsI GCATC 1 cut(s) 207
BmtI GCTAGC 2 cut(s) 206, 456
BpuMI CCSGG 1 cut(s) 38
BsaJI CCNNGG 3 cut(s) 100, 427, 464
BsaWI WCCGGW 1 cut(s) 21
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse1I ACTGG 4 cut(s) 14, 155, 394, 526
BseBI CCWGG 3 cut(s) 85, 222, 429
BseDI CCNNGG 3 cut(s) 100, 427, 464
BseGI GGATG 2 cut(s) 151, 390
BseLI CCNNNNNNNGG 1 cut(s) 396
BseNI ACTGG 4 cut(s) 14, 155, 394, 526
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 3 cut(s) 289, 388, 447
BseYI CCCAGC 2 cut(s) 361, 625
Bsh1236I CGCG 1 cut(s) 647
BshNI GGYRCC 1 cut(s) 423
BsiSI CCGG 2 cut(s) 22, 37
BslI CCNNNNNNNGG 1 cut(s) 396
BsmAI GTCTC 1 cut(s) 124
BsmBI CGTCTC 1 cut(s) 124
BsmI GAATGC 1 cut(s) 352
Bsp143I GATC 3 cut(s) 30, 51, 133
Bsp19I CCATGG 1 cut(s) 464
BspACI CCGC 3 cut(s) 68, 630, 647
BspFNI CGCG 1 cut(s) 647
BspLI GGNNCC 2 cut(s) 26, 425
BspOI GCTAGC 2 cut(s) 206, 456
BspT107I GGYRCC 1 cut(s) 423
BsrI ACTGG 4 cut(s) 14, 155, 394, 526
BssECI CCNNGG 3 cut(s) 100, 427, 464
BssMI GATC 3 cut(s) 30, 51, 133
BssT1I CCWWGG 1 cut(s) 464
Bst2UI CCWGG 3 cut(s) 85, 222, 429
Bst4CI ACNGT 2 cut(s) 347, 514
BstC8I GCNNGC 4 cut(s) 204, 373, 454, 458
BstDSI CCRYGG 1 cut(s) 464
BstF5I GGATG 2 cut(s) 151, 390
BstFNI CGCG 1 cut(s) 647
BstHHI GCGC 1 cut(s) 625
BstKTI GATC 3 cut(s) 33, 54, 136
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 3 cut(s) 30, 51, 133
BstMWI GCNNNNNNNGC 5 cut(s) 68, 203, 358, 466, 653
BstNI CCWGG 3 cut(s) 85, 222, 429
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 4 cut(s) 36, 83, 220, 427
BstSFI CTRYAG 1 cut(s) 343
BstUI CGCG 1 cut(s) 647
BstV1I GCAGC 3 cut(s) 289, 388, 447
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuI GTATCC 1 cut(s) 536
BtgI CCRYGG 1 cut(s) 464
BtsCI GGATG 2 cut(s) 151, 390
BtsI GCAGTG 2 cut(s) 48, 69
BtsIMutI CAGTG 4 cut(s) 48, 69, 162, 401
Cac8I GCNNGC 4 cut(s) 204, 373, 454, 458
CfoI GCGC 1 cut(s) 625
Cfr13I GGNCC 2 cut(s) 76, 87
Csp6I GTAC 1 cut(s) 424
CviAII CATG 5 cut(s) 91, 199, 465, 470, 560
CviQI GTAC 1 cut(s) 424
DpnI GATC 3 cut(s) 32, 53, 135
DpnII GATC 3 cut(s) 30, 51, 133
Eco130I CCWWGG 1 cut(s) 464
Eco47I GGWCC 2 cut(s) 76, 87
EcoRII CCWGG 3 cut(s) 83, 220, 427
EcoT14I CCWWGG 1 cut(s) 464
EcoT22I ATGCAT 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 464
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 5 cut(s) 94, 202, 468, 473, 563
FatI CATG 5 cut(s) 90, 198, 464, 469, 559
Fnu4HI GCNGC 4 cut(s) 69, 303, 402, 461
FokI GGATG 2 cut(s) 138, 377
Fsp4HI GCNGC 4 cut(s) 69, 303, 402, 461
FspBI CTAG 4 cut(s) 203, 368, 453, 530
GlaI GCGC 1 cut(s) 624
GluI GCNGC 4 cut(s) 69, 303, 402, 461
GsaI CCCAGC 2 cut(s) 365, 629
HapII CCGG 2 cut(s) 22, 37
HhaI GCGC 1 cut(s) 625
Hin1II CATG 5 cut(s) 94, 202, 468, 473, 563
Hin6I GCGC 1 cut(s) 623
HinP1I GCGC 1 cut(s) 623
HindIII AAGCTT 2 cut(s) 232, 585
HinfI GANTC 3 cut(s) 10, 174, 686
HpaII CCGG 2 cut(s) 22, 37
HphI GGTGA 4 cut(s) 178, 203, 242, 683
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 4 cut(s) 56, 101, 124, 683
Hpy188III TCNNGA 3 cut(s) 28, 79, 238
Hpy8I GTNNAC 1 cut(s) 250
HpyCH4III ACNGT 2 cut(s) 347, 514
HpyCH4IV ACGT 1 cut(s) 571
HpyCH4V TGCA 3 cut(s) 94, 352, 497
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 203, 358, 466, 653
HpySE526I ACGT 1 cut(s) 571
Hsp92II CATG 5 cut(s) 94, 202, 468, 473, 563
HspAI GCGC 1 cut(s) 623
KpnI GGTACC 1 cut(s) 427
Kzo9I GATC 3 cut(s) 30, 51, 133
LmnI GCTCC 1 cut(s) 661
Lsp1109I GCAGC 3 cut(s) 289, 388, 447
LweI GCATC 1 cut(s) 207
MaeI CTAG 4 cut(s) 203, 368, 453, 530
MaeII ACGT 1 cut(s) 571
MaeIII GTNAC 4 cut(s) 412, 508, 514, 671
MalI GATC 3 cut(s) 32, 53, 135
MboI GATC 3 cut(s) 30, 51, 133
MboII GAAGA 3 cut(s) 25, 133, 539
MflI RGATCY 1 cut(s) 133
MluCI AATT 4 cut(s) 242, 263, 337, 598
MlyI GAGTC 1 cut(s) 168
MnlI CCTC 2 cut(s) 95, 98
Mph1103I ATGCAT 1 cut(s) 96
MseI TTAA 2 cut(s) 185, 281
MspI CCGG 2 cut(s) 22, 37
MspR9I CCNGG 4 cut(s) 38, 85, 222, 429
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 3 cut(s) 85, 222, 429
MvnI CGCG 1 cut(s) 647
MwoI GCNNNNNNNGC 5 cut(s) 68, 203, 358, 466, 653
NciI CCSGG 1 cut(s) 38
NcoI CCATGG 1 cut(s) 464
NdeII GATC 3 cut(s) 30, 51, 133
NheI GCTAGC 2 cut(s) 202, 452
NlaIII CATG 5 cut(s) 94, 202, 468, 473, 563
NlaIV GGNNCC 2 cut(s) 26, 425
NmuCI GTSAC 1 cut(s) 671
NsiI ATGCAT 1 cut(s) 96
NspI RCATGY 1 cut(s) 473
PctI GAATGC 1 cut(s) 352
PfeI GAWTC 2 cut(s) 10, 686
PflMI CCANNNNNTGG 1 cut(s) 396
PkrI GCNGC 4 cut(s) 70, 304, 403, 462
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
Psp6I CCWGG 3 cut(s) 83, 220, 427
PspFI CCCAGC 2 cut(s) 361, 625
PspGI CCWGG 3 cut(s) 83, 220, 427
PspN4I GGNNCC 2 cut(s) 26, 425
PspPI GGNCC 2 cut(s) 76, 87
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
SaqAI TTAA 2 cut(s) 185, 281
SatI GCNGC 4 cut(s) 69, 303, 402, 461
Sau3AI GATC 3 cut(s) 30, 51, 133
Sau96I GGNCC 2 cut(s) 76, 87
SchI GAGTC 1 cut(s) 168
ScrFI CCNGG 4 cut(s) 38, 85, 222, 429
SfaNI GCATC 1 cut(s) 207
SfcI CTRYAG 1 cut(s) 343
SinI GGWCC 2 cut(s) 76, 87
Sse9I AATT 4 cut(s) 242, 263, 337, 598
SsiI CCGC 3 cut(s) 68, 630, 647
SspMI CTAG 4 cut(s) 203, 368, 453, 530
StyD4I CCNGG 4 cut(s) 36, 83, 220, 427
StyI CCWWGG 1 cut(s) 464
TaaI ACNGT 2 cut(s) 347, 514
TaiI ACGT 1 cut(s) 574
TaqI TCGA 1 cut(s) 437
TasI AATT 4 cut(s) 242, 263, 337, 598
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 2 cut(s) 10, 686
Tru1I TTAA 2 cut(s) 185, 281
Tru9I TTAA 2 cut(s) 185, 281
TscAI CASTG 4 cut(s) 48, 69, 162, 401
TseFI GTSAC 1 cut(s) 671
TseI GCWGC 3 cut(s) 302, 401, 460
Tsp45I GTSAC 1 cut(s) 671
TspDTI ATGAA 1 cut(s) 140
TspRI CASTG 4 cut(s) 48, 69, 162, 401
Van91I CCANNNNNTGG 1 cut(s) 396
VpaK11BI GGWCC 2 cut(s) 76, 87
XapI RAATTY 1 cut(s) 598
XceI RCATGY 1 cut(s) 473
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XspI CTAG 4 cut(s) 203, 368, 453, 530
Zsp2I ATGCAT 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.