RLG00000021387

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017157
Physical Location & Seq
Reverse (-)
74767454 .. 74769150
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021387

Sequence Viewer

Length: 789 bp
ATGGGGGCGATTCCAGTAGTCCGGTTCCCGATCTTCCGGGCAGTGAGAGTGATCGGAGTAGCAGTGGCGGCCCTAGTCCTGACCTGGACTATTCATTTCCGAGGAGGCTTGGCTCTCATCTCTGACAACAAAGATCTCATCTTCAATGTTCATCCAGTGTTGATGGTGATTGGACTCGTAATCTTAAATGGTGAAGCCATGCTAGCATACAAGACGGTGCCAGGAACAAAACGTTTCAAGAAATTAGTTCACCTTACGCTACAATTCCTGGCTTTTTGTTTAAGCATTATCGGTGTATGGGCTGCTTTGAAGTTCCACAATGATAAGGGCATTGACAATTTCTACAGTCTGCATTCTTGGCTGGGTCTAGCTTGCCTGTTCCTTTTTACCGTCCAGTGGGCTGCTGGATTTGTTACATATTGGTACCCAGGTGGCTCAAAAAATGGTAGAGCTAGCTTGCTGCCATGGCATGTCTTTTTCGGTGTTTATATATATGCACTCGCTGTTGTTACTGTAACTACCGGTATCCTAGAAAAAGCCACATTTCTTCAAGCCAACCATGTAATATCACGTTATTCAACTGAAGCTTTGCTGATAAATTCTCTGGGATTATTGGTCGTTGCTCTGGGCGGTTTTGTTATTCTCGCAGTTATTGCTCCTGCTAATAAAGGTGACATTTTCAGAGAATCAATAGACCAAAGTGATTCATTAATACCAGAAGAGGAATTAGAAGATCATGCTGAAGATGGGGCTTCAGCCATGGTGACACCTCATCTTCCCGACAAATAA

Protein Analysis

263

Amino Acids

28.69

Weight (kDa)

7.13

Isoelectric Point (pI)

30.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 50 - 182 5.1e-49 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38630
fragaria_vesca FvH4_6g45440 FvH4_6g45440 FvH4_6g45440
malus_domestica MD17G1073300.v1.1
prunus_persica Prupe.3G240600_v2.0.a1
pyrus_communis pycom17g07420
rosa_chinensis RchiOBHm_Chr2g0163271
rosa_laevigata RLG00000021387
rosa_roxburghii Rroxscaffold_2G00087420
rosa_rugosa Rorug02G0501900
rosa_samantha Rh2BG580800
rosa_wichuraiana Rw2G047050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 423
AccB1I GGYRCC 2 cut(s) 217, 423
AciI CCGC 2 cut(s) 68, 630
AclI AACGTT 1 cut(s) 232
AcsI RAATTY 1 cut(s) 598
AcuI CTGAAG 3 cut(s) 603, 738, 762
AfaI GTAC 1 cut(s) 425
AfiI CCNNNNNNNGG 1 cut(s) 396
AgeI ACCGGT 1 cut(s) 521
AgsI TTSAA 5 cut(s) 145, 238, 310, 551, 579
AjnI CCWGG 4 cut(s) 83, 220, 267, 427
AluBI AGCT 4 cut(s) 371, 452, 456, 587
AluI AGCT 4 cut(s) 371, 452, 456, 587
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 3 cut(s) 302, 401, 460
ApoI RAATTY 1 cut(s) 598
AseI ATTAAT 1 cut(s) 710
AsiGI ACCGGT 1 cut(s) 521
Asp718I GGTACC 1 cut(s) 423
AspS9I GGNCC 1 cut(s) 70
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 5 cut(s) 178, 203, 242, 683, 775
AsuNHI GCTAGC 2 cut(s) 202, 452
BaeI ACNNNNGTAYC 2 cut(s) 415, 448
BanI GGYRCC 2 cut(s) 217, 423
BbvI GCAGC 3 cut(s) 289, 388, 447
BccI CCATC 2 cut(s) 157, 740
BciT130I CCWGG 4 cut(s) 85, 222, 269, 429
BciVI GTATCC 1 cut(s) 536
BcnI CCSGG 1 cut(s) 38
BfaI CTAG 5 cut(s) 74, 203, 368, 453, 530
BfmI CTRYAG 1 cut(s) 343
BfuI GTATCC 1 cut(s) 536
BglII AGATCT 1 cut(s) 133
BisI GCNGC 4 cut(s) 69, 303, 402, 461
BlsI GCNGC 4 cut(s) 70, 304, 403, 462
Bme1390I CCNGG 5 cut(s) 38, 85, 222, 269, 429
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 3 cut(s) 26, 219, 425
BmrFI CCNGG 5 cut(s) 38, 85, 222, 269, 429
BmtI GCTAGC 2 cut(s) 206, 456
BpuMI CCSGG 1 cut(s) 38
BsaJI CCNNGG 4 cut(s) 100, 427, 464, 759
BsaWI WCCGGW 2 cut(s) 21, 521
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse118I RCCGGY 1 cut(s) 521
Bse1I ACTGG 3 cut(s) 14, 155, 394
BseBI CCWGG 4 cut(s) 85, 222, 269, 429
BseDI CCNNGG 4 cut(s) 100, 427, 464, 759
BseGI GGATG 1 cut(s) 151
BseLI CCNNNNNNNGG 1 cut(s) 396
BseNI ACTGG 3 cut(s) 14, 155, 394
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 3 cut(s) 289, 388, 447
BseYI CCCAGC 1 cut(s) 361
BshFI GGCC 1 cut(s) 71
BshNI GGYRCC 2 cut(s) 217, 423
BshTI ACCGGT 1 cut(s) 521
BsiSI CCGG 3 cut(s) 22, 37, 522
BslI CCNNNNNNNGG 1 cut(s) 396
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 1 cut(s) 71
Bsp143I GATC 4 cut(s) 30, 51, 133, 733
Bsp19I CCATGG 2 cut(s) 464, 759
BspACI CCGC 2 cut(s) 68, 630
BspANI GGCC 1 cut(s) 71
BspLI GGNNCC 3 cut(s) 26, 219, 425
BspOI GCTAGC 2 cut(s) 206, 456
BspT107I GGYRCC 2 cut(s) 217, 423
BsrFI RCCGGY 1 cut(s) 521
BsrI ACTGG 3 cut(s) 14, 155, 394
BssAI RCCGGY 1 cut(s) 521
BssECI CCNNGG 4 cut(s) 100, 427, 464, 759
BssMI GATC 4 cut(s) 30, 51, 133, 733
BssT1I CCWWGG 2 cut(s) 464, 759
Bst2UI CCWGG 4 cut(s) 85, 222, 269, 429
Bst4CI ACNGT 4 cut(s) 217, 347, 391, 514
Bst6I CTCTTC 1 cut(s) 714
BstAPI GCANNNNNTGC 1 cut(s) 653
BstC8I GCNNGC 4 cut(s) 204, 373, 454, 458
BstDSI CCRYGG 2 cut(s) 464, 759
BstF5I GGATG 1 cut(s) 151
BstKTI GATC 4 cut(s) 33, 54, 136, 736
BstMBI GATC 4 cut(s) 30, 51, 133, 733
BstMWI GCNNNNNNNGC 5 cut(s) 68, 203, 358, 466, 653
BstNI CCWGG 4 cut(s) 85, 222, 269, 429
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 5 cut(s) 36, 83, 220, 267, 427
BstSFI CTRYAG 1 cut(s) 343
BstV1I GCAGC 3 cut(s) 289, 388, 447
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuI GTATCC 1 cut(s) 536
BsuRI GGCC 1 cut(s) 71
BtgI CCRYGG 2 cut(s) 464, 759
BtsCI GGATG 1 cut(s) 151
BtsI GCAGTG 2 cut(s) 48, 69
BtsIMutI CAGTG 4 cut(s) 48, 69, 162, 401
Cac8I GCNNGC 4 cut(s) 204, 373, 454, 458
Cfr10I RCCGGY 1 cut(s) 521
Cfr13I GGNCC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 424
CspAI ACCGGT 1 cut(s) 521
CviAII CATG 6 cut(s) 199, 465, 470, 560, 737, 760
CviQI GTAC 1 cut(s) 424
DpnI GATC 4 cut(s) 32, 53, 135, 735
DpnII GATC 4 cut(s) 30, 51, 133, 733
Eam1104I CTCTTC 1 cut(s) 714
EarI CTCTTC 1 cut(s) 714
Eco130I CCWWGG 2 cut(s) 464, 759
Eco57I CTGAAG 3 cut(s) 603, 738, 762
EcoRII CCWGG 4 cut(s) 83, 220, 267, 427
EcoT14I CCWWGG 2 cut(s) 464, 759
ErhI CCWWGG 2 cut(s) 464, 759
FaeI CATG 6 cut(s) 202, 468, 473, 563, 740, 763
FatI CATG 6 cut(s) 198, 464, 469, 559, 736, 759
Fnu4HI GCNGC 4 cut(s) 69, 303, 402, 461
FokI GGATG 1 cut(s) 138
Fsp4HI GCNGC 4 cut(s) 69, 303, 402, 461
FspBI CTAG 5 cut(s) 74, 203, 368, 453, 530
GluI GCNGC 4 cut(s) 69, 303, 402, 461
GsaI CCCAGC 1 cut(s) 365
HaeIII GGCC 1 cut(s) 71
HapII CCGG 3 cut(s) 22, 37, 522
Hin1II CATG 6 cut(s) 202, 468, 473, 563, 740, 763
HindIII AAGCTT 1 cut(s) 585
HinfI GANTC 4 cut(s) 10, 174, 686, 704
HpaII CCGG 3 cut(s) 22, 37, 522
HphI GGTGA 5 cut(s) 178, 203, 242, 683, 775
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 4 cut(s) 56, 101, 124, 683
Hpy188III TCNNGA 4 cut(s) 28, 79, 238, 779
Hpy8I GTNNAC 1 cut(s) 250
HpyCH4III ACNGT 4 cut(s) 217, 347, 391, 514
HpyCH4IV ACGT 2 cut(s) 232, 571
HpyCH4V TGCA 2 cut(s) 352, 497
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 203, 358, 466, 653
HpySE526I ACGT 2 cut(s) 232, 571
Hsp92II CATG 6 cut(s) 202, 468, 473, 563, 740, 763
KpnI GGTACC 1 cut(s) 427
Kzo9I GATC 4 cut(s) 30, 51, 133, 733
LmnI GCTCC 1 cut(s) 661
Lsp1109I GCAGC 3 cut(s) 289, 388, 447
MaeI CTAG 5 cut(s) 74, 203, 368, 453, 530
MaeII ACGT 2 cut(s) 232, 571
MaeIII GTNAC 5 cut(s) 412, 508, 514, 671, 763
MalI GATC 4 cut(s) 32, 53, 135, 735
MboI GATC 4 cut(s) 30, 51, 133, 733
MboII GAAGA 7 cut(s) 25, 133, 539, 731, 743, 755, 767
MflI RGATCY 1 cut(s) 133
MluCI AATT 5 cut(s) 242, 263, 337, 598, 725
MlyI GAGTC 1 cut(s) 168
MnlI CCTC 4 cut(s) 95, 98, 715, 780
MseI TTAA 3 cut(s) 185, 281, 710
MspI CCGG 3 cut(s) 22, 37, 522
MspR9I CCNGG 5 cut(s) 38, 85, 222, 269, 429
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 4 cut(s) 85, 222, 269, 429
MwoI GCNNNNNNNGC 5 cut(s) 68, 203, 358, 466, 653
NciI CCSGG 1 cut(s) 38
NcoI CCATGG 2 cut(s) 464, 759
NdeII GATC 4 cut(s) 30, 51, 133, 733
NheI GCTAGC 2 cut(s) 202, 452
NlaIII CATG 6 cut(s) 202, 468, 473, 563, 740, 763
NlaIV GGNNCC 3 cut(s) 26, 219, 425
NmuCI GTSAC 2 cut(s) 671, 763
NspI RCATGY 1 cut(s) 473
PctI GAATGC 1 cut(s) 352
PfeI GAWTC 3 cut(s) 10, 686, 704
PinAI ACCGGT 1 cut(s) 521
PkrI GCNGC 4 cut(s) 70, 304, 403, 462
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
PshBI ATTAAT 1 cut(s) 710
Psp1406I AACGTT 1 cut(s) 232
Psp6I CCWGG 4 cut(s) 83, 220, 267, 427
PspFI CCCAGC 1 cut(s) 361
PspGI CCWGG 4 cut(s) 83, 220, 267, 427
PspN4I GGNNCC 3 cut(s) 26, 219, 425
PspPI GGNCC 1 cut(s) 70
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
SaqAI TTAA 3 cut(s) 185, 281, 710
SatI GCNGC 4 cut(s) 69, 303, 402, 461
Sau3AI GATC 4 cut(s) 30, 51, 133, 733
Sau96I GGNCC 1 cut(s) 70
SchI GAGTC 1 cut(s) 168
ScrFI CCNGG 5 cut(s) 38, 85, 222, 269, 429
SfcI CTRYAG 1 cut(s) 343
Sse9I AATT 5 cut(s) 242, 263, 337, 598, 725
SsiI CCGC 2 cut(s) 68, 630
SspMI CTAG 5 cut(s) 74, 203, 368, 453, 530
StyD4I CCNGG 5 cut(s) 36, 83, 220, 267, 427
StyI CCWWGG 2 cut(s) 464, 759
TaaI ACNGT 4 cut(s) 217, 347, 391, 514
TaiI ACGT 2 cut(s) 235, 574
TasI AATT 5 cut(s) 242, 263, 337, 598, 725
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 3 cut(s) 10, 686, 704
Tru1I TTAA 3 cut(s) 185, 281, 710
Tru9I TTAA 3 cut(s) 185, 281, 710
TscAI CASTG 4 cut(s) 48, 69, 162, 401
TseFI GTSAC 2 cut(s) 671, 763
TseI GCWGC 3 cut(s) 302, 401, 460
Tsp45I GTSAC 2 cut(s) 671, 763
TspDTI ATGAA 3 cut(s) 83, 140, 696
TspRI CASTG 4 cut(s) 48, 69, 162, 401
VspI ATTAAT 1 cut(s) 710
XapI RAATTY 1 cut(s) 598
XceI RCATGY 1 cut(s) 473
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XspI CTAG 5 cut(s) 74, 203, 368, 453, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.