MD17G1073300.v1.1

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0017157
Physical Location & Seq
Forward (+)
5898255 .. 5900486
2232 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1073300.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGCGGCGGTTCCGGTCGTCCGGTTCCCGATCTTGAGTGCAGTGAGGGTTATAGGAGTGATAGTGGCGACTCTGGTGCTCACGTGGAACGTTCATTTCCGCGGTGGATTGGCTCTCATCTCCGATGACAAAAGTCTTATTTTCAATGTGCATCCAGTATTAATGGTGATTGGGTTGATACTGCTCAATGGAGAAGCCATGCTAGCATACAAGACGGTTTCAGGAACAAAAAACTTGAAAAAACTAGTTCATCTTACACTACAATTCCTTGCCTTTTGCGTAAGCATCATCGGCGTATGGGCTGCTTTGAAGTTCCACAACGATAAGGGCATTGACAACTTCTACAGTCTGCATTCCTGGTTGGGTCTAGCTTGCCTATTCCTCTTCACCCTCCAGTGGGCTGCTGGATTTGTAACGTATTGGTTCCCAGGAGGCTCGAAACACAGCAGAGCTAACTTGCTGCCATGGCATGTATTTTTCGGTGTTTATACTTACGCCCTTGCTGTTGTTACAGTAACTACTGGTATTTTAGAAAAAGTCACCTTCCTCCAAACCCACCAAGTAATCTCACGCTATTCGACCGAGGCTTTGCTCGTGAACTCTCTCGGAATATTGGTCATTGTTCTGGGAGGTTTTGTTATCCTTGCCGTTATTACTCCGATGAATGATAATAGAGGTGATGTTCTCAGAGGACCAATAGAGTAA

Protein Analysis

235

Amino Acids

25.72

Weight (kDa)

9.48

Isoelectric Point (pI)

24.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 50 - 182 3.6e-48 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38630
fragaria_vesca FvH4_6g45440 FvH4_6g45440 FvH4_6g45440
malus_domestica MD17G1073300.v1.1
prunus_persica Prupe.3G240600_v2.0.a1
pyrus_communis pycom17g07420
rosa_chinensis RchiOBHm_Chr2g0163271
rosa_laevigata RLG00000021387
rosa_roxburghii Rroxscaffold_2G00087420
rosa_rugosa Rorug02G0501900
rosa_samantha Rh2BG580800
rosa_wichuraiana Rw2G047050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 102
AciI CCGC 4 cut(s) 5, 8, 100, 102
AclI AACGTT 1 cut(s) 90
AcvI CACGTG 1 cut(s) 84
AfiI CCNNNNNNNGG 2 cut(s) 396, 397
AgsI TTSAA 3 cut(s) 145, 238, 310
AhlI ACTAGT 1 cut(s) 244
AjnI CCWGG 2 cut(s) 356, 427
AluBI AGCT 2 cut(s) 371, 452
AluI AGCT 2 cut(s) 371, 452
Alw21I GWGCWC 1 cut(s) 81
ApeKI GCWGC 3 cut(s) 302, 401, 460
ArsI GACNNNNNNTTYG 2 cut(s) 571, 603
AseI ATTAAT 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 692
AsuHPI GGTGA 4 cut(s) 178, 379, 532, 689
AsuNHI GCTAGC 1 cut(s) 202
AvaII GGWCC 1 cut(s) 692
BauI CACGAG 1 cut(s) 593
BbrPI CACGTG 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 3 cut(s) 289, 388, 447
BceAI ACGGC 1 cut(s) 632
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciT130I CCWGG 2 cut(s) 358, 429
BcuI ACTAGT 1 cut(s) 244
BfaI CTAG 3 cut(s) 203, 245, 368
BfmI CTRYAG 1 cut(s) 343
BisI GCNGC 4 cut(s) 6, 303, 402, 461
BlsI GCNGC 4 cut(s) 7, 304, 403, 462
Bme1390I CCNGG 2 cut(s) 358, 429
Bme18I GGWCC 1 cut(s) 692
BmgT120I GGNCC 1 cut(s) 692
BmiI GGNNCC 3 cut(s) 12, 26, 425
BmrFI CCNGG 2 cut(s) 358, 429
BmsI GCATC 2 cut(s) 160, 294
BmtI GCTAGC 1 cut(s) 206
BoxI GACNNNNGTC 1 cut(s) 132
BpmI CTGGAG 1 cut(s) 377
BpuEI CTTGAG 1 cut(s) 55
BsaAI YACGTR 1 cut(s) 84
BsaJI CCNNGG 4 cut(s) 100, 427, 464, 582
BsaWI WCCGGW 2 cut(s) 13, 21
BsaXI ACNNNNNCTCC 4 cut(s) 48, 78, 621, 651
Bsc4I CCNNNNNNNGG 2 cut(s) 396, 397
Bse1I ACTGG 3 cut(s) 155, 394, 526
BseBI CCWGG 2 cut(s) 358, 429
BseDI CCNNGG 4 cut(s) 100, 427, 464, 582
BseGI GGATG 1 cut(s) 151
BseLI CCNNNNNNNGG 2 cut(s) 396, 397
BseMII CTCAG 1 cut(s) 700
BseNI ACTGG 3 cut(s) 155, 394, 526
BseXI GCAGC 3 cut(s) 289, 388, 447
BsgI GTGCAG 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 102
Bsh1285I CGRYCG 2 cut(s) 18, 582
BsiEI CGRYCG 2 cut(s) 18, 582
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 2 cut(s) 14, 22
BslI CCNNNNNNNGG 2 cut(s) 396, 397
BsmI GAATGC 1 cut(s) 352
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 1 cut(s) 30
Bsp19I CCATGG 1 cut(s) 464
BspACI CCGC 4 cut(s) 5, 8, 100, 102
BspCNI CTCAG 1 cut(s) 699
BspFNI CGCG 1 cut(s) 102
BspLI GGNNCC 3 cut(s) 12, 26, 425
BspOI GCTAGC 1 cut(s) 206
BsrI ACTGG 3 cut(s) 155, 394, 526
BssECI CCNNGG 4 cut(s) 100, 427, 464, 582
BssMI GATC 1 cut(s) 30
BssSI CACGAG 1 cut(s) 593
BssT1I CCWWGG 1 cut(s) 464
Bst2BI CACGAG 1 cut(s) 593
Bst2UI CCWGG 2 cut(s) 358, 429
Bst4CI ACNGT 3 cut(s) 217, 347, 514
Bst6I CTCTTC 1 cut(s) 389
BstBAI YACGTR 1 cut(s) 84
BstC8I GCNNGC 2 cut(s) 204, 373
BstDEI CTNAG 1 cut(s) 686
BstDSI CCRYGG 2 cut(s) 100, 464
BstF5I GGATG 1 cut(s) 151
BstFNI CGCG 1 cut(s) 102
BstKTI GATC 1 cut(s) 33
BstMBI GATC 1 cut(s) 30
BstMCI CGRYCG 2 cut(s) 18, 582
BstMWI GCNNNNNNNGC 3 cut(s) 203, 291, 466
BstNI CCWGG 2 cut(s) 358, 429
BstNSI RCATGY 1 cut(s) 473
BstPAI GACNNNNGTC 1 cut(s) 132
BstSCI CCNGG 2 cut(s) 356, 427
BstSFI CTRYAG 1 cut(s) 343
BstUI CGCG 1 cut(s) 102
BstV1I GCAGC 3 cut(s) 289, 388, 447
BtgI CCRYGG 2 cut(s) 100, 464
BtsCI GGATG 1 cut(s) 151
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 2 cut(s) 48, 401
Cac8I GCNNGC 2 cut(s) 204, 373
Cfr13I GGNCC 1 cut(s) 692
Cfr42I CCGCGG 1 cut(s) 103
CviAII CATG 3 cut(s) 199, 465, 470
CviJI RGCY 8 cut(s) 113, 197, 302, 371, 401, 435, 452, 587
CviKI_1 RGCY 8 cut(s) 113, 197, 302, 371, 401, 435, 452, 587
DdeI CTNAG 1 cut(s) 686
DpnI GATC 1 cut(s) 32
DpnII GATC 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 389
EarI CTCTTC 1 cut(s) 389
Eco130I CCWWGG 1 cut(s) 464
Eco47I GGWCC 1 cut(s) 692
Eco72I CACGTG 1 cut(s) 84
EcoRII CCWGG 2 cut(s) 356, 427
EcoT14I CCWWGG 1 cut(s) 464
ErhI CCWWGG 1 cut(s) 464
FaeI CATG 3 cut(s) 202, 468, 473
FaiI YATR 7 cut(s) 53, 200, 208, 298, 466, 471, 489
FatI CATG 3 cut(s) 198, 464, 469
Fnu4HI GCNGC 4 cut(s) 6, 303, 402, 461
FokI GGATG 1 cut(s) 138
Fsp4HI GCNGC 4 cut(s) 6, 303, 402, 461
FspBI CTAG 3 cut(s) 203, 245, 368
GluI GCNGC 4 cut(s) 6, 303, 402, 461
GsuI CTGGAG 1 cut(s) 377
HapII CCGG 2 cut(s) 14, 22
Hin1II CATG 3 cut(s) 202, 468, 473
HinfI GANTC 1 cut(s) 70
HpaII CCGG 2 cut(s) 14, 22
HphI GGTGA 4 cut(s) 178, 379, 532, 689
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 4 cut(s) 124, 608, 660, 689
Hpy188III TCNNGA 4 cut(s) 28, 34, 222, 595
Hpy8I GTNNAC 1 cut(s) 598
HpyAV CCTTC 1 cut(s) 553
HpyCH4III ACNGT 3 cut(s) 217, 347, 514
HpyCH4IV ACGT 3 cut(s) 83, 90, 416
HpyCH4V TGCA 3 cut(s) 41, 151, 352
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 291, 466
HpyF3I CTNAG 1 cut(s) 686
HpySE526I ACGT 3 cut(s) 83, 90, 416
Hsp92II CATG 3 cut(s) 202, 468, 473
KspI CCGCGG 1 cut(s) 103
Kzo9I GATC 1 cut(s) 30
Lsp1109I GCAGC 3 cut(s) 289, 388, 447
LweI GCATC 2 cut(s) 160, 294
MaeI CTAG 3 cut(s) 203, 245, 368
MaeII ACGT 3 cut(s) 83, 90, 416
MaeIII GTNAC 4 cut(s) 412, 508, 514, 538
MalI GATC 1 cut(s) 32
MboI GATC 1 cut(s) 30
MboII GAAGA 1 cut(s) 376
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 1 cut(s) 263
MlyI GAGTC 1 cut(s) 64
MnlI CCTC 9 cut(s) 39, 392, 401, 425, 557, 577, 623, 668, 683
MseI TTAA 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 102
MspI CCGG 2 cut(s) 14, 22
MspR9I CCNGG 2 cut(s) 358, 429
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 2 cut(s) 358, 429
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 3 cut(s) 203, 291, 466
NcoI CCATGG 1 cut(s) 464
NdeII GATC 1 cut(s) 30
NheI GCTAGC 1 cut(s) 202
NlaIII CATG 3 cut(s) 202, 468, 473
NlaIV GGNNCC 3 cut(s) 12, 26, 425
NmuCI GTSAC 1 cut(s) 538
NspI RCATGY 1 cut(s) 473
PctI GAATGC 1 cut(s) 352
PkrI GCNGC 4 cut(s) 7, 304, 403, 462
PleI GAGTC 1 cut(s) 64
PmaCI CACGTG 1 cut(s) 84
PmlI CACGTG 1 cut(s) 84
PpsI GAGTC 1 cut(s) 64
Ppu21I YACGTR 1 cut(s) 84
PshAI GACNNNNGTC 1 cut(s) 132
PshBI ATTAAT 1 cut(s) 161
Psp1406I AACGTT 1 cut(s) 90
Psp6I CCWGG 2 cut(s) 356, 427
PspCI CACGTG 1 cut(s) 84
PspGI CCWGG 2 cut(s) 356, 427
PspN4I GGNNCC 3 cut(s) 12, 26, 425
PspPI GGNCC 1 cut(s) 692
SacII CCGCGG 1 cut(s) 103
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 4 cut(s) 6, 303, 402, 461
Sau3AI GATC 1 cut(s) 30
Sau96I GGNCC 1 cut(s) 692
SchI GAGTC 1 cut(s) 64
ScrFI CCNGG 2 cut(s) 358, 429
SduI GDGCHC 1 cut(s) 81
SetI ASST 8 cut(s) 86, 93, 373, 419, 454, 545, 634, 679
SfaNI GCATC 2 cut(s) 160, 294
SfcI CTRYAG 1 cut(s) 343
Sfr303I CCGCGG 1 cut(s) 103
SgrBI CCGCGG 1 cut(s) 103
SinI GGWCC 1 cut(s) 692
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
SpeI ACTAGT 1 cut(s) 244
Sse9I AATT 1 cut(s) 263
SsiI CCGC 4 cut(s) 5, 8, 100, 102
SspI AATATT 1 cut(s) 612
SspMI CTAG 3 cut(s) 203, 245, 368
StyD4I CCNGG 2 cut(s) 356, 427
StyI CCWWGG 1 cut(s) 464
TaaI ACNGT 3 cut(s) 217, 347, 514
TaiI ACGT 3 cut(s) 86, 93, 419
TaqI TCGA 2 cut(s) 437, 578
TaqII GACCGA 1 cut(s) 596
TasI AATT 1 cut(s) 263
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TscAI CASTG 2 cut(s) 48, 401
TseFI GTSAC 1 cut(s) 538
TseI GCWGC 3 cut(s) 302, 401, 460
Tsp45I GTSAC 1 cut(s) 538
TspDTI ATGAA 3 cut(s) 83, 239, 677
TspRI CASTG 2 cut(s) 48, 401
VpaK11BI GGWCC 1 cut(s) 692
VspI ATTAAT 1 cut(s) 161
XceI RCATGY 1 cut(s) 473
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XspI CTAG 3 cut(s) 203, 245, 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.