AT5G39600

39S ribosomal protein L53/MRP-L53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002943
Physical Location & Seq
Reverse (-)
15853929 .. 15855192
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G39600.1

Sequence Viewer

Length: 384 bp
ATGTTGAAGTTTCTATCAAAAGTAAGAATTGAGTTCAACACATTAGATCCAAGACTTGCGTCTTGTGTTGAATTCTTAGCTCAATGCAATGCAAGGAAGGCCAAAGAATCGAATCCCAATTGTCAGGTTTTGGTGAAACGTAGAACCGATGAGCAGCCGCCGCAGATCACTGTCACTTTTGTTAATGGTGTTGAGGAAGCTTTTGATGCAGCTGCAACCTCGGCTCAGTCGATTAGAAAGATGATTCTTGATAAAGGTCAGTATCTTGAGACAGAGCAAATGTTTCGTGAAGCTGGAGAACAATGGCCTGTGATTATCCCTGATGAAGAACTTCTCCAAGAGGCTCCTGGTGTTAAGCCGAGAAAAGCAGAAGACAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP_L53

Protein Analysis

127

Amino Acids

14.44

Weight (kDa)

5.62

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP_L53 PF10780 12 - 63 6.1e-15 39S ribosomal protein L53/MRP-L53
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 158, 161
AclWI GGATC 1 cut(s) 41
AcsI RAATTY 1 cut(s) 71
AgsI TTSAA 3 cut(s) 7, 37, 71
AjnI CCWGG 1 cut(s) 346
AluBI AGCT 4 cut(s) 80, 200, 212, 293
AluI AGCT 4 cut(s) 80, 200, 212, 293
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 1 cut(s) 41
AoxI GGCC 2 cut(s) 99, 305
ApeKI GCWGC 3 cut(s) 154, 209, 212
ApoI RAATTY 1 cut(s) 71
Asp700I GAANNNNTTC 1 cut(s) 330
AsuHPI GGTGA 1 cut(s) 145
BbsI GAAGAC 1 cut(s) 378
BbvI GCAGC 3 cut(s) 166, 199, 221
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BciT130I CCWGG 1 cut(s) 348
BcoDI GTCTC 1 cut(s) 263
BisI GCNGC 5 cut(s) 155, 158, 161, 210, 213
BlsI GCNGC 5 cut(s) 156, 159, 162, 211, 214
Bme1390I CCNGG 1 cut(s) 348
BmiI GGNNCC 1 cut(s) 345
BmrFI CCNGG 1 cut(s) 348
BmsI GCATC 1 cut(s) 196
BoxI GACNNNNGTC 1 cut(s) 58
BpiI GAAGAC 1 cut(s) 378
BpmI CTGGAG 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 219
Bse3DI GCAATG 1 cut(s) 94
BseBI CCWGG 1 cut(s) 348
BseDI CCNNGG 1 cut(s) 219
BseMI GCAATG 1 cut(s) 94
BseMII CTCAG 1 cut(s) 239
BseXI GCAGC 3 cut(s) 166, 199, 221
BshFI GGCC 2 cut(s) 101, 307
BsmAI GTCTC 1 cut(s) 263
BsnI GGCC 2 cut(s) 101, 307
Bsp143I GATC 2 cut(s) 46, 165
BspACI CCGC 2 cut(s) 158, 161
BspANI GGCC 2 cut(s) 101, 307
BspCNI CTCAG 1 cut(s) 238
BspLI GGNNCC 1 cut(s) 345
BspPI GGATC 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 94
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 2 cut(s) 46, 165
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 1 cut(s) 172
BstDEI CTNAG 2 cut(s) 76, 225
BstKTI GATC 2 cut(s) 49, 168
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 2 cut(s) 46, 165
BstMWI GCNNNNNNNGC 4 cut(s) 98, 160, 206, 221
BstNI CCWGG 1 cut(s) 348
BstPAI GACNNNNGTC 1 cut(s) 58
BstSCI CCNGG 1 cut(s) 346
BstV1I GCAGC 3 cut(s) 166, 199, 221
BstV2I GAAGAC 1 cut(s) 378
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BsuRI GGCC 2 cut(s) 101, 307
BtsIMutI CAGTG 1 cut(s) 168
CseI GACGC 1 cut(s) 48
DdeI CTNAG 2 cut(s) 76, 225
DpnI GATC 2 cut(s) 48, 167
DpnII GATC 2 cut(s) 46, 165
EcoRI GAATTC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 346
Fnu4HI GCNGC 5 cut(s) 155, 158, 161, 210, 213
Fsp4HI GCNGC 5 cut(s) 155, 158, 161, 210, 213
GluI GCNGC 5 cut(s) 155, 158, 161, 210, 213
GsuI CTGGAG 1 cut(s) 315
HaeIII GGCC 2 cut(s) 101, 307
HgaI GACGC 1 cut(s) 48
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 3 cut(s) 107, 112, 244
HphI GGTGA 1 cut(s) 145
Hpy188III TCNNGA 3 cut(s) 248, 266, 287
HpyAV CCTTC 1 cut(s) 91
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 139
HpyCH4V TGCA 4 cut(s) 87, 92, 209, 215
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 160, 206, 221
HpyF3I CTNAG 2 cut(s) 76, 225
HpySE526I ACGT 1 cut(s) 139
Kzo9I GATC 2 cut(s) 46, 165
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 6 cut(s) 110, 279, 321, 333, 333, 360
Lsp1109I GCAGC 3 cut(s) 166, 199, 221
LweI GCATC 1 cut(s) 196
MaeII ACGT 1 cut(s) 139
MaeIII GTNAC 1 cut(s) 172
MalI GATC 2 cut(s) 48, 167
MboI GATC 2 cut(s) 46, 165
MboII GAAGA 2 cut(s) 338, 383
MfeI CAATTG 1 cut(s) 118
MflI RGATCY 1 cut(s) 46
MluCI AATT 3 cut(s) 27, 71, 118
MnlI CCTC 3 cut(s) 187, 229, 334
MroXI GAANNNNTTC 1 cut(s) 330
MseI TTAA 2 cut(s) 183, 354
MspA1I CMGCKG 1 cut(s) 212
MspR9I CCNGG 1 cut(s) 348
MunI CAATTG 1 cut(s) 118
MvaI CCWGG 1 cut(s) 348
MwoI GCNNNNNNNGC 4 cut(s) 98, 160, 206, 221
NdeII GATC 2 cut(s) 46, 165
NlaIV GGNNCC 1 cut(s) 345
NmeAIII GCCGAG 2 cut(s) 200, 384
NmuCI GTSAC 1 cut(s) 172
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 330
PfeI GAWTC 3 cut(s) 107, 112, 244
PkrI GCNGC 5 cut(s) 156, 159, 162, 211, 214
PshAI GACNNNNGTC 1 cut(s) 58
Psp6I CCWGG 1 cut(s) 346
PspGI CCWGG 1 cut(s) 346
PspN4I GGNNCC 1 cut(s) 345
PsuI RGATCY 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 212
SaqAI TTAA 2 cut(s) 183, 354
SatI GCNGC 5 cut(s) 155, 158, 161, 210, 213
Sau3AI GATC 2 cut(s) 46, 165
ScrFI CCNGG 1 cut(s) 348
SetI ASST 8 cut(s) 82, 129, 142, 202, 214, 221, 259, 295
SfaNI GCATC 1 cut(s) 196
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 3 cut(s) 27, 71, 118
SsiI CCGC 2 cut(s) 158, 161
StyD4I CCNGG 1 cut(s) 346
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 1 cut(s) 142
TaqI TCGA 2 cut(s) 110, 230
TasI AATT 3 cut(s) 27, 71, 118
TauI GCSGC 2 cut(s) 160, 163
TfiI GAWTC 3 cut(s) 107, 112, 244
Tru1I TTAA 2 cut(s) 183, 354
Tru9I TTAA 2 cut(s) 183, 354
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 3 cut(s) 154, 209, 212
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 339
TspRI CASTG 1 cut(s) 175
XapI RAATTY 1 cut(s) 71
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.