FvH4_1g25620

39S ribosomal protein L53/MRP-L53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002943
Physical Location & Seq
Reverse (-)
17466889 .. 17471539
4651 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25620.t13

Sequence Viewer

Length: 687 bp
ATGTTGAAGTTCCTCTCGCAAGTGAGGATCGAGTTCAATGCATTGGACCCACGCACAGCTTCGTGCATGGAGTTCTTGGCGCAGTGCAACGCTCCCAAGGCCAAGGAGTCCAATCCCAGCTGCGCCGTCCAGGTCAAGCGCCGCACCGACGACGAGCCGCTCAAGATCATCGTCACCTTCGCCAACGGAGTCAAGGAGGTCTTCGACGCCACTGCCATGCCCGCTCAGAGCATCAGGACCATGATTCTTGAGAAGGGTCGTATGCTTGAGACCGAGCAGATGTTCCACGATGTCAGTGTGGCCTGGCCGGTGCTTATTCTGGCGGAGGAGCTCACCCAGCCGGTGCCTGGTACCAAGAGGGAGCTTATGTCTTCATGTTGTGGATACATGCATAGACCATTCCACAGCCTATTCTATATGCAGCCTCCCAATGCTGTTAGGGAGACGGAAGGCCTTTCTTTTGCCAAGGAAAGCAGAAGAGAAGAAGCTGTAGCCTCTATTTTGTTCAATCTACATATTATGTGGGATAAGGATGGCATTTTGTTCCATTGGGTAATTACCCACCCATTAAACAAAATGGGTAATACCCACTGGGTAAATACCCATCATCTTTTTTCATGGGTTTTTTCATACCCACCCTCATGGGTAACGGGTACCCTACCCCCATATCCCCTAACAACCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP_L53

Protein Analysis

229

Amino Acids

26.09

Weight (kDa)

6.75

Isoelectric Point (pI)

60.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP_L53 PF10780 12 - 63 1.5e-12 39S ribosomal protein L53/MRP-L53
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 350, 653
AccB1I GGYRCC 3 cut(s) 343, 350, 653
AccBSI CCGCTC 2 cut(s) 160, 224
AciI CCGC 4 cut(s) 142, 158, 222, 323
AclWI GGATC 1 cut(s) 35
AcoI YGGCCR 1 cut(s) 305
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 2 cut(s) 352, 655
AfiI CCNNNNNNNGG 1 cut(s) 347
AgsI TTSAA 3 cut(s) 7, 37, 508
AjnI CCWGG 3 cut(s) 129, 302, 346
AluBI AGCT 5 cut(s) 59, 120, 331, 364, 488
AluI AGCT 5 cut(s) 59, 120, 331, 364, 488
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 2 cut(s) 263, 437
AlwI GGATC 1 cut(s) 35
AoxI GGCC 4 cut(s) 99, 300, 305, 451
ApeKI GCWGC 2 cut(s) 120, 421
Asp718I GGTACC 2 cut(s) 350, 653
AspLEI GCGC 3 cut(s) 82, 125, 141
AspS9I GGNCC 2 cut(s) 46, 237
AsuHPI GGTGA 2 cut(s) 166, 325
AvaII GGWCC 2 cut(s) 46, 237
BanI GGYRCC 3 cut(s) 343, 350, 653
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 2 cut(s) 193, 363
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 2 cut(s) 107, 433
BccI CCATC 2 cut(s) 527, 612
BceAI ACGGC 1 cut(s) 110
BcgI CGANNNNNNTGC 4 cut(s) 20, 54, 194, 228
BciT130I CCWGG 3 cut(s) 131, 304, 348
BciVI GTATCC 1 cut(s) 377
BcoDI GTCTC 2 cut(s) 263, 437
BfmI CTRYAG 1 cut(s) 489
BfoI RGCGCY 1 cut(s) 142
BfuI GTATCC 1 cut(s) 377
BisI GCNGC 4 cut(s) 121, 142, 158, 422
BlsI GCNGC 4 cut(s) 122, 143, 159, 423
Bme1390I CCNGG 3 cut(s) 131, 304, 348
Bme18I GGWCC 2 cut(s) 46, 237
BmgT120I GGNCC 2 cut(s) 46, 237
BmiI GGNNCC 4 cut(s) 48, 345, 352, 655
BmrFI CCNGG 3 cut(s) 131, 304, 348
BmrI ACTGGG 1 cut(s) 601
BmsI GCATC 1 cut(s) 240
BmuI ACTGGG 1 cut(s) 601
BpiI GAAGAC 2 cut(s) 193, 363
BpuEI CTTGAG 3 cut(s) 146, 269, 287
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 3 cut(s) 96, 102, 465
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 1 cut(s) 347
Bse118I RCCGGY 2 cut(s) 307, 340
Bse1I ACTGG 1 cut(s) 596
BseBI CCWGG 3 cut(s) 131, 304, 348
BseDI CCNNGG 3 cut(s) 96, 102, 465
BseGI GGATG 1 cut(s) 538
BseLI CCNNNNNNNGG 1 cut(s) 347
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 1 cut(s) 596
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 2 cut(s) 107, 433
BseYI CCCAGC 2 cut(s) 116, 336
BshFI GGCC 4 cut(s) 101, 302, 307, 453
BshNI GGYRCC 3 cut(s) 343, 350, 653
BsiHKAI GWGCWC 1 cut(s) 333
BsiSI CCGG 2 cut(s) 308, 341
BslI CCNNNNNNNGG 1 cut(s) 347
BsmAI GTCTC 2 cut(s) 263, 437
BsmBI CGTCTC 1 cut(s) 437
BsnI GGCC 4 cut(s) 101, 302, 307, 453
Bso31I GGTCTC 1 cut(s) 263
Bsp1286I GDGCHC 1 cut(s) 333
Bsp143I GATC 2 cut(s) 27, 165
BspACI CCGC 4 cut(s) 142, 158, 222, 323
BspANI GGCC 4 cut(s) 101, 302, 307, 453
BspCNI CTCAG 1 cut(s) 238
BspLI GGNNCC 4 cut(s) 48, 345, 352, 655
BspPI GGATC 1 cut(s) 35
BspT107I GGYRCC 3 cut(s) 343, 350, 653
BspTNI GGTCTC 1 cut(s) 263
BsrBI CCGCTC 2 cut(s) 160, 224
BsrFI RCCGGY 2 cut(s) 307, 340
BsrI ACTGG 1 cut(s) 596
BssAI RCCGGY 2 cut(s) 307, 340
BssECI CCNNGG 3 cut(s) 96, 102, 465
BssMI GATC 2 cut(s) 27, 165
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 3 cut(s) 96, 102, 465
Bst2UI CCWGG 3 cut(s) 131, 304, 348
Bst6I CTCTTC 1 cut(s) 472
BstACI GRCGYC 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 1 cut(s) 225
BstF5I GGATG 1 cut(s) 538
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 3 cut(s) 82, 125, 141
BstKTI GATC 2 cut(s) 30, 168
BstMAI GTCTC 2 cut(s) 263, 437
BstMBI GATC 2 cut(s) 27, 165
BstMWI GCNNNNNNNGC 3 cut(s) 98, 221, 337
BstNI CCWGG 3 cut(s) 131, 304, 348
BstNSI RCATGY 1 cut(s) 391
BstSCI CCNGG 3 cut(s) 129, 302, 346
BstSFI CTRYAG 1 cut(s) 489
BstV1I GCAGC 2 cut(s) 107, 433
BstV2I GAAGAC 2 cut(s) 193, 363
BstXI CCANNNNNNTGG 1 cut(s) 642
BsuI GTATCC 1 cut(s) 377
BsuRI GGCC 4 cut(s) 101, 302, 307, 453
BtsCI GGATG 1 cut(s) 538
BtsI GCAGTG 2 cut(s) 89, 210
BtsIMutI CAGTG 4 cut(s) 89, 210, 301, 589
Cac8I GCNNGC 1 cut(s) 222
CfoI GCGC 3 cut(s) 82, 125, 141
Cfr10I RCCGGY 2 cut(s) 307, 340
Cfr13I GGNCC 2 cut(s) 46, 237
CseI GACGC 1 cut(s) 215
Csp6I GTAC 2 cut(s) 351, 654
CviAII CATG 7 cut(s) 67, 217, 241, 375, 388, 618, 642
CviQI GTAC 2 cut(s) 351, 654
DdeI CTNAG 1 cut(s) 225
DpnI GATC 2 cut(s) 29, 167
DpnII GATC 2 cut(s) 27, 165
EaeI YGGCCR 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 472
EarI CTCTTC 1 cut(s) 472
EciI GGCGGA 1 cut(s) 338
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 3 cut(s) 96, 102, 465
Eco147I AGGCCT 1 cut(s) 453
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 263
Eco47I GGWCC 2 cut(s) 46, 237
Eco53kI GAGCTC 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 331
EcoRII CCWGG 3 cut(s) 129, 302, 346
EcoT14I CCWWGG 3 cut(s) 96, 102, 465
EcoT22I ATGCAT 2 cut(s) 43, 393
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 3 cut(s) 96, 102, 465
Esp3I CGTCTC 1 cut(s) 437
FaeI CATG 7 cut(s) 70, 220, 244, 378, 391, 621, 645
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FatI CATG 7 cut(s) 66, 216, 240, 374, 387, 617, 641
FauI CCCGC 1 cut(s) 229
Fnu4HI GCNGC 4 cut(s) 121, 142, 158, 422
FokI GGATG 1 cut(s) 545
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 4 cut(s) 121, 142, 158, 422
GlaI GCGC 3 cut(s) 81, 124, 140
GluI GCNGC 4 cut(s) 121, 142, 158, 422
GsaI CCCAGC 2 cut(s) 120, 340
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 4 cut(s) 101, 302, 307, 453
HapII CCGG 2 cut(s) 308, 341
HgaI GACGC 1 cut(s) 215
HhaI GCGC 3 cut(s) 82, 125, 141
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 7 cut(s) 70, 220, 244, 378, 391, 621, 645
Hin6I GCGC 3 cut(s) 80, 123, 139
HinP1I GCGC 3 cut(s) 80, 123, 139
HinfI GANTC 3 cut(s) 107, 189, 244
HpaII CCGG 2 cut(s) 308, 341
HphI GGTGA 2 cut(s) 166, 325
Hpy188I TCNGA 1 cut(s) 228
Hpy188III TCNNGA 3 cut(s) 163, 235, 248
Hpy99I CGWCG 3 cut(s) 152, 155, 209
HpyAV CCTTC 3 cut(s) 187, 247, 443
HpyCH4V TGCA 5 cut(s) 41, 66, 87, 391, 421
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 221, 337
HpyF3I CTNAG 1 cut(s) 225
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 7 cut(s) 70, 220, 244, 378, 391, 621, 645
HspAI GCGC 3 cut(s) 80, 123, 139
KpnI GGTACC 2 cut(s) 354, 657
Kzo9I GATC 2 cut(s) 27, 165
LmnI GCTCC 3 cut(s) 97, 328, 361
Lsp1109I GCAGC 2 cut(s) 107, 433
LweI GCATC 1 cut(s) 240
MaeIII GTNAC 2 cut(s) 172, 646
MalI GATC 2 cut(s) 29, 167
MbiI CCGCTC 2 cut(s) 160, 224
MboI GATC 2 cut(s) 27, 165
MboII GAAGA 4 cut(s) 193, 363, 489, 494
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 1 cut(s) 555
MlyI GAGTC 2 cut(s) 116, 198
MnlI CCTC 8 cut(s) 18, 23, 190, 319, 351, 435, 505, 649
Mph1103I ATGCAT 2 cut(s) 43, 393
MseI TTAA 1 cut(s) 569
MslI CAYNNNNRTG 2 cut(s) 215, 640
MspA1I CMGCKG 1 cut(s) 120
MspI CCGG 2 cut(s) 308, 341
MspR9I CCNGG 3 cut(s) 131, 304, 348
MvaI CCWGG 3 cut(s) 131, 304, 348
MwoI GCNNNNNNNGC 3 cut(s) 98, 221, 337
NdeII GATC 2 cut(s) 27, 165
NlaIII CATG 7 cut(s) 70, 220, 244, 378, 391, 621, 645
NlaIV GGNNCC 4 cut(s) 48, 345, 352, 655
NmuCI GTSAC 1 cut(s) 172
NsiI ATGCAT 2 cut(s) 43, 393
NspI RCATGY 1 cut(s) 391
PceI AGGCCT 1 cut(s) 453
PfeI GAWTC 1 cut(s) 244
PkrI GCNGC 4 cut(s) 122, 143, 159, 423
PleI GAGTC 2 cut(s) 115, 197
PpsI GAGTC 2 cut(s) 115, 197
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 3 cut(s) 129, 302, 346
PspFI CCCAGC 2 cut(s) 116, 336
PspGI CCWGG 3 cut(s) 129, 302, 346
PspN4I GGNNCC 4 cut(s) 48, 345, 352, 655
PspPI GGNCC 2 cut(s) 46, 237
PvuII CAGCTG 1 cut(s) 120
RsaI GTAC 2 cut(s) 352, 655
RsaNI GTAC 2 cut(s) 351, 654
RseI CAYNNNNRTG 2 cut(s) 215, 640
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 1 cut(s) 569
SatI GCNGC 4 cut(s) 121, 142, 158, 422
Sau3AI GATC 2 cut(s) 27, 165
Sau96I GGNCC 2 cut(s) 46, 237
SchI GAGTC 2 cut(s) 116, 198
ScrFI CCNGG 3 cut(s) 131, 304, 348
SduI GDGCHC 1 cut(s) 333
SetI ASST 8 cut(s) 61, 122, 135, 179, 201, 333, 366, 490
SfaNI GCATC 1 cut(s) 240
SfcI CTRYAG 1 cut(s) 489
SinI GGWCC 2 cut(s) 46, 237
SmiMI CAYNNNNRTG 2 cut(s) 215, 640
SmlI CTYRAG 3 cut(s) 161, 248, 266
SmoI CTYRAG 3 cut(s) 161, 248, 266
Sse9I AATT 1 cut(s) 555
SseBI AGGCCT 1 cut(s) 453
SsiI CCGC 4 cut(s) 142, 158, 222, 323
SstI GAGCTC 1 cut(s) 333
StuI AGGCCT 1 cut(s) 453
StyD4I CCNGG 3 cut(s) 129, 302, 346
StyI CCWWGG 3 cut(s) 96, 102, 465
TaqI TCGA 2 cut(s) 30, 204
TaqII GACCGA 1 cut(s) 287
TasI AATT 1 cut(s) 555
TauI GCSGC 2 cut(s) 144, 160
TfiI GAWTC 1 cut(s) 244
Tru1I TTAA 1 cut(s) 569
Tru9I TTAA 1 cut(s) 569
TscAI CASTG 4 cut(s) 89, 217, 301, 596
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 2 cut(s) 120, 421
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 3 cut(s) 363, 606, 618
TspGWI ACGGA 2 cut(s) 201, 461
TspRI CASTG 4 cut(s) 89, 217, 301, 596
VpaK11BI GGWCC 2 cut(s) 46, 237
XceI RCATGY 1 cut(s) 391
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
Zsp2I ATGCAT 2 cut(s) 43, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.