MD02G1241500.v1.1

39S ribosomal protein L53/MRP-L53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002943
Physical Location & Seq
Forward (+)
29016010 .. 29018345
2336 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1241500.v1.1.491

Sequence Viewer

Length: 387 bp
ATGTTGAAGTTCCTCTCGAGAGTGAGGATCGAGTTCAATGCCTTGGACCCCCGAACGGCGTCGTGTCTGGAGTTCCTGGCGCAGTGCAACGCCCGCAAGGCCAAGGAGTCCAACCCCTCCTGCGCCGTCCAAGTCAAGCGCCGCACCGACGACCAGCCCCCCAAGATCACCGTCACCTTCGTCAATGGCGTCGAGCAGGTCTACGACGCCACCGCCACTCCCGCCCAGGACATCCGCAACATGATCCTCGAAAAGGGTCAGAGCCTCGAGACCGAGCAGATGTTCCGCGACGCCGGCGAGTCCTGGCCCGTTATTATCCCCGCCGACGAGCTCCACCAACCGGCCCCCGGCACCAAGCCGAAGAAAGCGGAAGAGAAAAAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP_L53

Protein Analysis

129

Amino Acids

14.38

Weight (kDa)

7.69

Isoelectric Point (pI)

54.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP_L53 PF10780 12 - 63 4.3e-15 39S ribosomal protein L53/MRP-L53
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 187
AccB1I GGYRCC 1 cut(s) 350
AccI GTMKAC 1 cut(s) 201
AccII CGCG 1 cut(s) 288
AciI CCGC 8 cut(s) 94, 142, 213, 222, 235, 286, 321, 368
AclWI GGATC 2 cut(s) 35, 238
AcyI GRCGYC 4 cut(s) 59, 189, 207, 291
AfiI CCNNNNNNNGG 4 cut(s) 55, 253, 340, 347
AgsI TTSAA 2 cut(s) 7, 37
AjnI CCWGG 3 cut(s) 75, 225, 302
AluBI AGCT 1 cut(s) 331
AluI AGCT 1 cut(s) 331
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 2 cut(s) 35, 238
Ama87I CYCGRG 2 cut(s) 16, 266
AoxI GGCC 3 cut(s) 99, 305, 342
AspLEI GCGC 3 cut(s) 82, 125, 141
AspS9I GGNCC 3 cut(s) 46, 306, 343
AsuC2I CCSGG 1 cut(s) 348
AsuHPI GGTGA 2 cut(s) 160, 166
AvaI CYCGRG 2 cut(s) 16, 266
AvaII GGWCC 1 cut(s) 46
BanI GGYRCC 1 cut(s) 350
BanII GRGCYC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 333
BceAI ACGGC 2 cut(s) 72, 110
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 3 cut(s) 77, 227, 304
BcnI CCSGG 1 cut(s) 348
BcoDI GTCTC 1 cut(s) 263
BfoI RGCGCY 1 cut(s) 142
BfuAI ACCTGC 1 cut(s) 187
BglI GCCNNNNNGGC 1 cut(s) 98
BisI GCNGC 1 cut(s) 142
BlsI GCNGC 1 cut(s) 143
Bme1390I CCNGG 4 cut(s) 77, 227, 304, 348
Bme18I GGWCC 1 cut(s) 46
BmeT110I CYCGRG 2 cut(s) 16, 266
BmgT120I GGNCC 3 cut(s) 46, 306, 343
BmiI GGNNCC 3 cut(s) 48, 345, 352
BmrFI CCNGG 4 cut(s) 77, 227, 304, 348
BpmI CTGGAG 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 348
BsaHI GRCGYC 4 cut(s) 59, 189, 207, 291
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 4 cut(s) 42, 102, 225, 346
BsaXI ACNNNNNCTCC 2 cut(s) 202, 232
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 253, 340, 347
Bse118I RCCGGY 2 cut(s) 293, 340
BseBI CCWGG 3 cut(s) 77, 227, 304
BseDI CCNNGG 4 cut(s) 42, 102, 225, 346
BseGI GGATG 1 cut(s) 231
BseLI CCNNNNNNNGG 4 cut(s) 55, 253, 340, 347
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 3 cut(s) 101, 307, 344
BshNI GGYRCC 1 cut(s) 350
BsiHKAI GWGCWC 1 cut(s) 333
BsiHKCI CYCGRG 2 cut(s) 16, 266
BsiSI CCGG 3 cut(s) 294, 341, 348
BslI CCNNNNNNNGG 4 cut(s) 55, 253, 340, 347
BsmAI GTCTC 1 cut(s) 263
BsnI GGCC 3 cut(s) 101, 307, 344
Bso31I GGTCTC 1 cut(s) 263
BsoBI CYCGRG 2 cut(s) 16, 266
Bsp1286I GDGCHC 1 cut(s) 333
Bsp143I GATC 3 cut(s) 27, 165, 243
BspACI CCGC 8 cut(s) 94, 142, 213, 222, 235, 286, 321, 368
BspANI GGCC 3 cut(s) 101, 307, 344
BspFNI CGCG 1 cut(s) 288
BspLI GGNNCC 3 cut(s) 48, 345, 352
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 2 cut(s) 35, 238
BspT107I GGYRCC 1 cut(s) 350
BspTNI GGTCTC 1 cut(s) 263
BsrFI RCCGGY 2 cut(s) 293, 340
BssAI RCCGGY 2 cut(s) 293, 340
BssECI CCNNGG 4 cut(s) 42, 102, 225, 346
BssMI GATC 3 cut(s) 27, 165, 243
BssNI GRCGYC 4 cut(s) 59, 189, 207, 291
BssT1I CCWWGG 2 cut(s) 42, 102
Bst2UI CCWGG 3 cut(s) 77, 227, 304
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 1 cut(s) 366
BstACI GRCGYC 4 cut(s) 59, 189, 207, 291
BstC8I GCNNGC 2 cut(s) 94, 295
BstENI CCTNNNNNAGG 1 cut(s) 251
BstF5I GGATG 1 cut(s) 231
BstFNI CGCG 1 cut(s) 288
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 3 cut(s) 82, 125, 141
BstKTI GATC 3 cut(s) 30, 168, 246
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 3 cut(s) 27, 165, 243
BstMWI GCNNNNNNNGC 4 cut(s) 93, 98, 221, 294
BstNI CCWGG 3 cut(s) 77, 227, 304
BstSCI CCNGG 4 cut(s) 75, 225, 302, 346
BstUI CGCG 1 cut(s) 288
BsuRI GGCC 3 cut(s) 101, 307, 344
BtsCI GGATG 1 cut(s) 231
BtsI GCAGTG 1 cut(s) 89
BtsIMutI CAGTG 1 cut(s) 89
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 2 cut(s) 94, 295
CfoI GCGC 3 cut(s) 82, 125, 141
Cfr10I RCCGGY 2 cut(s) 293, 340
Cfr13I GGNCC 3 cut(s) 46, 306, 343
CseI GACGC 4 cut(s) 48, 178, 215, 299
CviAII CATG 1 cut(s) 241
CviJI RGCY 7 cut(s) 101, 157, 264, 307, 331, 344, 358
CviKI_1 RGCY 7 cut(s) 101, 157, 264, 307, 331, 344, 358
DpnI GATC 3 cut(s) 29, 167, 245
DpnII GATC 3 cut(s) 27, 165, 243
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 2 cut(s) 42, 102
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 263
Eco47I GGWCC 1 cut(s) 46
Eco53kI GAGCTC 1 cut(s) 331
Eco88I CYCGRG 2 cut(s) 16, 266
EcoICRI GAGCTC 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 251
EcoRII CCWGG 3 cut(s) 75, 225, 302
EcoT14I CCWWGG 2 cut(s) 42, 102
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 2 cut(s) 42, 102
FaeI CATG 1 cut(s) 244
FaiI YATR 1 cut(s) 242
FatI CATG 1 cut(s) 240
FauI CCCGC 3 cut(s) 101, 229, 328
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 1 cut(s) 142
FokI GGATG 1 cut(s) 218
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 1 cut(s) 142
GlaI GCGC 3 cut(s) 81, 124, 140
GluI GCNGC 1 cut(s) 142
GsuI CTGGAG 1 cut(s) 89
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 3 cut(s) 101, 307, 344
HapII CCGG 3 cut(s) 294, 341, 348
HgaI GACGC 4 cut(s) 48, 178, 215, 299
HhaI GCGC 3 cut(s) 82, 125, 141
Hin1I GRCGYC 4 cut(s) 59, 189, 207, 291
Hin1II CATG 1 cut(s) 244
Hin6I GCGC 3 cut(s) 80, 123, 139
HinP1I GCGC 3 cut(s) 80, 123, 139
HinfI GANTC 2 cut(s) 107, 299
HpaII CCGG 3 cut(s) 294, 341, 348
HphI GGTGA 2 cut(s) 160, 166
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 1 cut(s) 261
Hpy188III TCNNGA 4 cut(s) 16, 18, 68, 268
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 6 cut(s) 64, 152, 194, 209, 293, 329
HpyAV CCTTC 1 cut(s) 187
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4V TGCA 1 cut(s) 87
HpyF10VI GCNNNNNNNGC 4 cut(s) 93, 98, 221, 294
Hsp92I GRCGYC 4 cut(s) 59, 189, 207, 291
Hsp92II CATG 1 cut(s) 244
HspAI GCGC 3 cut(s) 80, 123, 139
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Kzo9I GATC 3 cut(s) 27, 165, 243
LmnI GCTCC 1 cut(s) 336
MaeIII GTNAC 1 cut(s) 172
MalI GATC 3 cut(s) 29, 167, 245
MboI GATC 3 cut(s) 27, 165, 243
MboII GAAGA 2 cut(s) 373, 383
MhlI GDGCHC 1 cut(s) 333
MlyI GAGTC 2 cut(s) 116, 308
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 5 cut(s) 18, 23, 127, 257, 275
MreI CGCCGGCG 1 cut(s) 293
MroNI GCCGGC 1 cut(s) 293
MspI CCGG 3 cut(s) 294, 341, 348
MspR9I CCNGG 4 cut(s) 77, 227, 304, 348
MvaI CCWGG 3 cut(s) 77, 227, 304
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 4 cut(s) 93, 98, 221, 294
NaeI GCCGGC 1 cut(s) 295
NciI CCSGG 1 cut(s) 348
NdeII GATC 3 cut(s) 27, 165, 243
NgoMIV GCCGGC 1 cut(s) 293
NlaIII CATG 1 cut(s) 244
NlaIV GGNNCC 3 cut(s) 48, 345, 352
NmuCI GTSAC 1 cut(s) 172
PaeR7I CTCGAG 2 cut(s) 16, 266
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PdiI GCCGGC 1 cut(s) 295
PkrI GCNGC 1 cut(s) 143
PleI GAGTC 2 cut(s) 115, 307
PpsI GAGTC 2 cut(s) 115, 307
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 3 cut(s) 75, 225, 302
PspGI CCWGG 3 cut(s) 75, 225, 302
PspN4I GGNNCC 3 cut(s) 48, 345, 352
PspPI GGNCC 3 cut(s) 46, 306, 343
SacI GAGCTC 1 cut(s) 333
SatI GCNGC 1 cut(s) 142
Sau3AI GATC 3 cut(s) 27, 165, 243
Sau96I GGNCC 3 cut(s) 46, 306, 343
SchI GAGTC 2 cut(s) 116, 308
ScrFI CCNGG 4 cut(s) 77, 227, 304, 348
SduI GDGCHC 1 cut(s) 333
SetI ASST 3 cut(s) 179, 201, 333
Sfr274I CTCGAG 2 cut(s) 16, 266
SgrAI CRCCGGYG 1 cut(s) 293
SinI GGWCC 1 cut(s) 46
SlaI CTCGAG 2 cut(s) 16, 266
SmlI CTYRAG 2 cut(s) 16, 266
SmoI CTYRAG 2 cut(s) 16, 266
SsiI CCGC 8 cut(s) 94, 142, 213, 222, 235, 286, 321, 368
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 4 cut(s) 75, 225, 302, 346
StyI CCWWGG 2 cut(s) 42, 102
TaaI ACNGT 1 cut(s) 172
TaqI TCGA 5 cut(s) 17, 30, 192, 249, 267
TaqII GACCGA 1 cut(s) 287
TauI GCSGC 1 cut(s) 144
TscAI CASTG 1 cut(s) 89
TseFI GTSAC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 172
TspRI CASTG 1 cut(s) 89
VpaK11BI GGWCC 1 cut(s) 46
XagI CCTNNNNNAGG 1 cut(s) 251
XhoI CTCGAG 2 cut(s) 16, 266
XmiI GTMKAC 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.