MD07G1075400.v1.1

39S ribosomal protein L53/MRP-L53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002943
Physical Location & Seq
Reverse (-)
7214357 .. 7217061
2705 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1075400.v1.1.491

Sequence Viewer

Length: 387 bp
ATGTTGAAGTTCCTATCGAGAGTGAGGATCGAGTTCAATGCCTTGGACCCTCGAACGGCATCCTGTCTGGAGTTCCTGGCGCAGTGCAACGCCCGCAAGGCCAAGGAGTCCAACCCCTCCTGCGCCGTCCAAGTCAAGCGCCGCACCGACGACCAGCCCCCCAAGATCACCGTCACCTTCGTCAACGGCGTCGAGCAGGTCTACGACGCCACCGCCACTCCCGCCCAGGATATCCGCAACATGATTCTCGAAAAGGGTCAGAGCCTCGAGACCGAGCAGATGTTCCGCGACGCCGGCGAGTCCTGGCCCGTTATTATCCCCAGCGGCGAGCTCCACCAACCGGCCCCCGGCACCAAGCCGAAGAAAGCTGAAGAGAAAAAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP_L53

Protein Analysis

129

Amino Acids

14.34

Weight (kDa)

8.47

Isoelectric Point (pI)

54.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP_L53 PF10780 12 - 63 4.3e-15 39S ribosomal protein L53/MRP-L53
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 187
AccB1I GGYRCC 1 cut(s) 350
AccI GTMKAC 1 cut(s) 201
AccII CGCG 1 cut(s) 288
AciI CCGC 7 cut(s) 94, 142, 213, 222, 235, 286, 324
AclWI GGATC 1 cut(s) 35
AcyI GRCGYC 3 cut(s) 189, 207, 291
AfiI CCNNNNNNNGG 3 cut(s) 55, 340, 347
AgsI TTSAA 2 cut(s) 7, 37
AjnI CCWGG 3 cut(s) 75, 225, 302
AluBI AGCT 2 cut(s) 331, 368
AluI AGCT 2 cut(s) 331, 368
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 1 cut(s) 35
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 3 cut(s) 99, 305, 342
AspLEI GCGC 3 cut(s) 82, 125, 141
AspS9I GGNCC 3 cut(s) 46, 306, 343
AsuC2I CCSGG 1 cut(s) 348
AsuHPI GGTGA 2 cut(s) 160, 166
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 1 cut(s) 46
BanI GGYRCC 1 cut(s) 350
BanII GRGCYC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 333
BceAI ACGGC 3 cut(s) 72, 110, 202
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 3 cut(s) 77, 227, 304
BcnI CCSGG 1 cut(s) 348
BcoDI GTCTC 1 cut(s) 263
BfoI RGCGCY 1 cut(s) 142
BfuAI ACCTGC 1 cut(s) 187
BglI GCCNNNNNGGC 1 cut(s) 98
BisI GCNGC 2 cut(s) 142, 325
BlsI GCNGC 2 cut(s) 143, 326
Bme1390I CCNGG 4 cut(s) 77, 227, 304, 348
Bme18I GGWCC 1 cut(s) 46
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 3 cut(s) 46, 306, 343
BmiI GGNNCC 3 cut(s) 48, 345, 352
BmrFI CCNGG 4 cut(s) 77, 227, 304, 348
BmsI GCATC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 348
BsaHI GRCGYC 3 cut(s) 189, 207, 291
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 4 cut(s) 42, 102, 225, 346
BsaXI ACNNNNNCTCC 2 cut(s) 202, 232
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 340, 347
Bse118I RCCGGY 2 cut(s) 293, 340
BseBI CCWGG 3 cut(s) 77, 227, 304
BseDI CCNNGG 4 cut(s) 42, 102, 225, 346
BseGI GGATG 1 cut(s) 59
BseLI CCNNNNNNNGG 3 cut(s) 55, 340, 347
BseYI CCCAGC 1 cut(s) 320
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 3 cut(s) 101, 307, 344
BshNI GGYRCC 1 cut(s) 350
BsiHKAI GWGCWC 1 cut(s) 333
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 3 cut(s) 294, 341, 348
BslI CCNNNNNNNGG 3 cut(s) 55, 340, 347
BsmAI GTCTC 1 cut(s) 263
BsnI GGCC 3 cut(s) 101, 307, 344
Bso31I GGTCTC 1 cut(s) 263
BsoBI CYCGRG 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 333
Bsp143I GATC 2 cut(s) 27, 165
BspACI CCGC 7 cut(s) 94, 142, 213, 222, 235, 286, 324
BspANI GGCC 3 cut(s) 101, 307, 344
BspFNI CGCG 1 cut(s) 288
BspLI GGNNCC 3 cut(s) 48, 345, 352
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 350
BspTNI GGTCTC 1 cut(s) 263
BsrFI RCCGGY 2 cut(s) 293, 340
BssAI RCCGGY 2 cut(s) 293, 340
BssECI CCNNGG 4 cut(s) 42, 102, 225, 346
BssMI GATC 2 cut(s) 27, 165
BssNI GRCGYC 3 cut(s) 189, 207, 291
BssT1I CCWWGG 2 cut(s) 42, 102
Bst2UI CCWGG 3 cut(s) 77, 227, 304
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 1 cut(s) 366
BstACI GRCGYC 3 cut(s) 189, 207, 291
BstC8I GCNNGC 3 cut(s) 94, 295, 329
BstF5I GGATG 1 cut(s) 59
BstFNI CGCG 1 cut(s) 288
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 3 cut(s) 82, 125, 141
BstKTI GATC 2 cut(s) 30, 168
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 2 cut(s) 27, 165
BstMWI GCNNNNNNNGC 4 cut(s) 93, 98, 221, 294
BstNI CCWGG 3 cut(s) 77, 227, 304
BstSCI CCNGG 4 cut(s) 75, 225, 302, 346
BstUI CGCG 1 cut(s) 288
BsuRI GGCC 3 cut(s) 101, 307, 344
BtsCI GGATG 1 cut(s) 59
BtsI GCAGTG 1 cut(s) 89
BtsIMutI CAGTG 1 cut(s) 89
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 3 cut(s) 94, 295, 329
CfoI GCGC 3 cut(s) 82, 125, 141
Cfr10I RCCGGY 2 cut(s) 293, 340
Cfr13I GGNCC 3 cut(s) 46, 306, 343
CseI GACGC 3 cut(s) 178, 215, 299
CviAII CATG 1 cut(s) 241
CviJI RGCY 8 cut(s) 101, 157, 264, 307, 331, 344, 358, 368
CviKI_1 RGCY 8 cut(s) 101, 157, 264, 307, 331, 344, 358, 368
DpnI GATC 2 cut(s) 29, 167
DpnII GATC 2 cut(s) 27, 165
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 2 cut(s) 42, 102
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 263
Eco32I GATATC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 46
Eco53kI GAGCTC 1 cut(s) 331
Eco88I CYCGRG 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 331
EcoRII CCWGG 3 cut(s) 75, 225, 302
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 2 cut(s) 42, 102
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 2 cut(s) 42, 102
FaeI CATG 1 cut(s) 244
FaiI YATR 1 cut(s) 242
FatI CATG 1 cut(s) 240
FauI CCCGC 2 cut(s) 101, 229
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 2 cut(s) 142, 325
FokI GGATG 1 cut(s) 46
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 2 cut(s) 142, 325
GlaI GCGC 3 cut(s) 81, 124, 140
GluI GCNGC 2 cut(s) 142, 325
GsaI CCCAGC 1 cut(s) 324
GsuI CTGGAG 1 cut(s) 89
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 3 cut(s) 101, 307, 344
HapII CCGG 3 cut(s) 294, 341, 348
HgaI GACGC 3 cut(s) 178, 215, 299
HhaI GCGC 3 cut(s) 82, 125, 141
Hin1I GRCGYC 3 cut(s) 189, 207, 291
Hin1II CATG 1 cut(s) 244
Hin6I GCGC 3 cut(s) 80, 123, 139
HinP1I GCGC 3 cut(s) 80, 123, 139
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 3 cut(s) 107, 244, 299
HpaII CCGG 3 cut(s) 294, 341, 348
HphI GGTGA 2 cut(s) 160, 166
Hpy166II GTNNAC 2 cut(s) 184, 202
Hpy188I TCNGA 1 cut(s) 261
Hpy188III TCNNGA 4 cut(s) 18, 68, 248, 268
Hpy8I GTNNAC 2 cut(s) 184, 202
Hpy99I CGWCG 4 cut(s) 152, 194, 209, 293
HpyAV CCTTC 1 cut(s) 187
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4V TGCA 1 cut(s) 87
HpyF10VI GCNNNNNNNGC 4 cut(s) 93, 98, 221, 294
Hsp92I GRCGYC 3 cut(s) 189, 207, 291
Hsp92II CATG 1 cut(s) 244
HspAI GCGC 3 cut(s) 80, 123, 139
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Kzo9I GATC 2 cut(s) 27, 165
LmnI GCTCC 1 cut(s) 336
LweI GCATC 1 cut(s) 68
MaeIII GTNAC 1 cut(s) 172
MalI GATC 2 cut(s) 29, 167
MboI GATC 2 cut(s) 27, 165
MboII GAAGA 2 cut(s) 373, 383
MhlI GDGCHC 1 cut(s) 333
MlyI GAGTC 2 cut(s) 116, 308
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 4 cut(s) 18, 60, 127, 275
MreI CGCCGGCG 1 cut(s) 293
MroNI GCCGGC 1 cut(s) 293
MspA1I CMGCKG 1 cut(s) 324
MspI CCGG 3 cut(s) 294, 341, 348
MspR9I CCNGG 4 cut(s) 77, 227, 304, 348
MvaI CCWGG 3 cut(s) 77, 227, 304
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 4 cut(s) 93, 98, 221, 294
NaeI GCCGGC 1 cut(s) 295
NciI CCSGG 1 cut(s) 348
NdeII GATC 2 cut(s) 27, 165
NgoMIV GCCGGC 1 cut(s) 293
NlaIII CATG 1 cut(s) 244
NlaIV GGNNCC 3 cut(s) 48, 345, 352
NmuCI GTSAC 1 cut(s) 172
PaeR7I CTCGAG 1 cut(s) 266
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PdiI GCCGGC 1 cut(s) 295
PfeI GAWTC 1 cut(s) 244
PkrI GCNGC 2 cut(s) 143, 326
PleI GAGTC 2 cut(s) 115, 307
PpsI GAGTC 2 cut(s) 115, 307
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 3 cut(s) 75, 225, 302
PspFI CCCAGC 1 cut(s) 320
PspGI CCWGG 3 cut(s) 75, 225, 302
PspN4I GGNNCC 3 cut(s) 48, 345, 352
PspPI GGNCC 3 cut(s) 46, 306, 343
SacI GAGCTC 1 cut(s) 333
SatI GCNGC 2 cut(s) 142, 325
Sau3AI GATC 2 cut(s) 27, 165
Sau96I GGNCC 3 cut(s) 46, 306, 343
SchI GAGTC 2 cut(s) 116, 308
ScrFI CCNGG 4 cut(s) 77, 227, 304, 348
SduI GDGCHC 1 cut(s) 333
SetI ASST 4 cut(s) 179, 201, 333, 370
SfaNI GCATC 1 cut(s) 68
Sfr274I CTCGAG 1 cut(s) 266
SgrAI CRCCGGYG 1 cut(s) 293
SinI GGWCC 1 cut(s) 46
SlaI CTCGAG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
SsiI CCGC 7 cut(s) 94, 142, 213, 222, 235, 286, 324
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 4 cut(s) 75, 225, 302, 346
StyI CCWWGG 2 cut(s) 42, 102
TaaI ACNGT 1 cut(s) 172
TaqI TCGA 6 cut(s) 17, 30, 52, 192, 249, 267
TaqII GACCGA 1 cut(s) 287
TauI GCSGC 2 cut(s) 144, 327
TfiI GAWTC 1 cut(s) 244
TscAI CASTG 1 cut(s) 89
TseFI GTSAC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 172
TspRI CASTG 1 cut(s) 89
VpaK11BI GGWCC 1 cut(s) 46
XhoI CTCGAG 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.