FvH4_2g38800

39S ribosomal protein L53/MRP-L53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002943
Physical Location & Seq
Reverse (-)
28001657 .. 28003495
1839 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38800.t1

Sequence Viewer

Length: 387 bp
ATGTTGAAGTTCCTCTCGCGCGTGAGGATCGAGTTCAACGCGTTGGACCCACGCACGGCTTCGTGCATGGAGTTCTTGGCGCAGTGCAACGCCCCCAAGGCCAAAGAGTCCAATCCCGGCTGCGCCGTCCAAGTCAAGCGCCGCACCGACGACGAACCCCCCAAGATCACCGTCACCTTCGCCAACGGAGTCGAGGAGGTCTTCGACGCGACCGCCACGCCTGCCCAGAGCATTCGGACCATGATTCTCGAAAAGGGTCGGATGCTTGAGACGGAGCAGATGTTCCGCGACTCCGGCGAGGCCTGGCCGGTGCTTATTCCGGCCGAGGAGCTCACCCAACCGGCGCCTGGTACCAAGCCTAGGAAAGCAGAAGAGAAGAAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP_L53

Protein Analysis

129

Amino Acids

14.31

Weight (kDa)

6.36

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP_L53 PF10780 12 - 63 5.5e-16 39S ribosomal protein L53/MRP-L53
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 350
AccB1I GGYRCC 2 cut(s) 343, 350
AccII CGCG 5 cut(s) 19, 21, 41, 209, 288
AciI CCGC 3 cut(s) 142, 213, 286
AclWI GGATC 1 cut(s) 35
AcoI YGGCCR 2 cut(s) 305, 321
AcyI GRCGYC 1 cut(s) 344
AfaI GTAC 1 cut(s) 352
AfiI CCNNNNNNNGG 3 cut(s) 55, 347, 360
AflIII ACRYGT 1 cut(s) 39
AgsI TTSAA 2 cut(s) 7, 37
AjnI CCWGG 2 cut(s) 302, 346
AloI GAACNNNNNNTCC 2 cut(s) 266, 298
AluBI AGCT 1 cut(s) 331
AluI AGCT 1 cut(s) 331
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 1 cut(s) 35
AoxI GGCC 4 cut(s) 99, 300, 305, 321
ApeKI GCWGC 1 cut(s) 120
Asp718I GGTACC 1 cut(s) 350
AspA2I CCTAGG 1 cut(s) 359
AspLEI GCGC 5 cut(s) 21, 82, 125, 141, 346
AspS9I GGNCC 2 cut(s) 46, 237
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 3 cut(s) 160, 166, 325
AvaII GGWCC 2 cut(s) 46, 237
AvrII CCTAGG 1 cut(s) 359
BanI GGYRCC 2 cut(s) 343, 350
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 193
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 1 cut(s) 107
BceAI ACGGC 2 cut(s) 72, 110
BciT130I CCWGG 2 cut(s) 304, 348
BcnI CCSGG 1 cut(s) 117
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 1 cut(s) 360
BfoI RGCGCY 2 cut(s) 142, 347
BglI GCCNNNNNGGC 1 cut(s) 98
BisI GCNGC 2 cut(s) 121, 142
BlnI CCTAGG 1 cut(s) 359
BlsI GCNGC 2 cut(s) 122, 143
Bme1390I CCNGG 3 cut(s) 117, 304, 348
Bme18I GGWCC 2 cut(s) 46, 237
BmgT120I GGNCC 2 cut(s) 46, 237
BmiI GGNNCC 3 cut(s) 48, 345, 352
BmrFI CCNGG 3 cut(s) 117, 304, 348
BmsI GCATC 1 cut(s) 252
BpiI GAAGAC 1 cut(s) 193
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 117
BsaHI GRCGYC 1 cut(s) 344
BsaJI CCNNGG 3 cut(s) 96, 324, 359
BsaXI ACNNNNNCTCC 2 cut(s) 266, 296
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 347, 360
Bse118I RCCGGY 2 cut(s) 307, 340
BseBI CCWGG 2 cut(s) 304, 348
BseDI CCNNGG 3 cut(s) 96, 324, 359
BseGI GGATG 1 cut(s) 267
BseLI CCNNNNNNNGG 3 cut(s) 55, 347, 360
BseRI GAGGAG 2 cut(s) 209, 341
BseX3I CGGCCG 1 cut(s) 321
BseXI GCAGC 1 cut(s) 107
Bsh1236I CGCG 5 cut(s) 19, 21, 41, 209, 288
Bsh1285I CGRYCG 2 cut(s) 213, 324
BshFI GGCC 4 cut(s) 101, 302, 307, 323
BshNI GGYRCC 2 cut(s) 343, 350
BsiEI CGRYCG 2 cut(s) 213, 324
BsiHKAI GWGCWC 1 cut(s) 333
BsiSI CCGG 5 cut(s) 117, 294, 308, 320, 341
BslI CCNNNNNNNGG 3 cut(s) 55, 347, 360
BsmAI GTCTC 1 cut(s) 263
BsmBI CGTCTC 1 cut(s) 263
BsmI GAATGC 1 cut(s) 231
BsnI GGCC 4 cut(s) 101, 302, 307, 323
Bsp1286I GDGCHC 1 cut(s) 333
Bsp143I GATC 2 cut(s) 27, 165
BspACI CCGC 3 cut(s) 142, 213, 286
BspANI GGCC 4 cut(s) 101, 302, 307, 323
BspFNI CGCG 5 cut(s) 19, 21, 41, 209, 288
BspLI GGNNCC 3 cut(s) 48, 345, 352
BspPI GGATC 1 cut(s) 35
BspT107I GGYRCC 2 cut(s) 343, 350
BsrFI RCCGGY 2 cut(s) 307, 340
BssAI RCCGGY 2 cut(s) 307, 340
BssECI CCNNGG 3 cut(s) 96, 324, 359
BssMI GATC 2 cut(s) 27, 165
BssNI GRCGYC 1 cut(s) 344
BssT1I CCWWGG 2 cut(s) 96, 359
Bst2UI CCWGG 2 cut(s) 304, 348
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 1 cut(s) 366
BstACI GRCGYC 1 cut(s) 344
BstC8I GCNNGC 1 cut(s) 222
BstF5I GGATG 1 cut(s) 267
BstFNI CGCG 5 cut(s) 19, 21, 41, 209, 288
BstH2I RGCGCY 2 cut(s) 142, 347
BstHHI GCGC 5 cut(s) 21, 82, 125, 141, 346
BstKTI GATC 2 cut(s) 30, 168
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 2 cut(s) 27, 165
BstMCI CGRYCG 2 cut(s) 213, 324
BstMWI GCNNNNNNNGC 3 cut(s) 98, 221, 294
BstNI CCWGG 2 cut(s) 304, 348
BstSCI CCNGG 3 cut(s) 115, 302, 346
BstUI CGCG 5 cut(s) 19, 21, 41, 209, 288
BstV1I GCAGC 1 cut(s) 107
BstV2I GAAGAC 1 cut(s) 193
BstZI CGGCCG 1 cut(s) 321
BsuRI GGCC 4 cut(s) 101, 302, 307, 323
BtsCI GGATG 1 cut(s) 267
BtsI GCAGTG 1 cut(s) 89
BtsIMutI CAGTG 1 cut(s) 89
Cac8I GCNNGC 1 cut(s) 222
CfoI GCGC 5 cut(s) 21, 82, 125, 141, 346
Cfr10I RCCGGY 2 cut(s) 307, 340
Cfr13I GGNCC 2 cut(s) 46, 237
CseI GACGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 351
CviAII CATG 2 cut(s) 67, 241
CviJI RGCY 8 cut(s) 59, 101, 120, 302, 307, 323, 331, 358
CviKI_1 RGCY 8 cut(s) 59, 101, 120, 302, 307, 323, 331, 358
CviQI GTAC 1 cut(s) 351
DinI GGCGCC 1 cut(s) 345
DpnI GATC 2 cut(s) 29, 167
DpnII GATC 2 cut(s) 27, 165
EaeI YGGCCR 2 cut(s) 305, 321
EagI CGGCCG 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
Ecl136II GAGCTC 1 cut(s) 331
EclXI CGGCCG 1 cut(s) 321
Eco130I CCWWGG 2 cut(s) 96, 359
Eco147I AGGCCT 1 cut(s) 302
Eco24I GRGCYC 1 cut(s) 333
Eco47I GGWCC 2 cut(s) 46, 237
Eco52I CGGCCG 1 cut(s) 321
Eco53kI GAGCTC 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 302, 346
EcoT14I CCWWGG 2 cut(s) 96, 359
EcoT38I GRGCYC 1 cut(s) 333
EgeI GGCGCC 1 cut(s) 345
EheI GGCGCC 1 cut(s) 345
ErhI CCWWGG 2 cut(s) 96, 359
Esp3I CGTCTC 1 cut(s) 263
FaeI CATG 2 cut(s) 70, 244
FaiI YATR 2 cut(s) 68, 242
FatI CATG 2 cut(s) 66, 240
Fnu4HI GCNGC 2 cut(s) 121, 142
FokI GGATG 1 cut(s) 274
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 2 cut(s) 121, 142
FspBI CTAG 1 cut(s) 360
GlaI GCGC 5 cut(s) 20, 81, 124, 140, 345
GluI GCNGC 2 cut(s) 121, 142
HaeII RGCGCY 2 cut(s) 142, 347
HaeIII GGCC 4 cut(s) 101, 302, 307, 323
HapII CCGG 5 cut(s) 117, 294, 308, 320, 341
HgaI GACGC 1 cut(s) 215
HhaI GCGC 5 cut(s) 21, 82, 125, 141, 346
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 2 cut(s) 70, 244
Hin6I GCGC 5 cut(s) 19, 80, 123, 139, 344
HinP1I GCGC 5 cut(s) 19, 80, 123, 139, 344
HinfI GANTC 4 cut(s) 107, 189, 244, 290
HpaII CCGG 5 cut(s) 117, 294, 308, 320, 341
HphI GGTGA 3 cut(s) 160, 166, 325
Hpy188I TCNGA 2 cut(s) 237, 261
Hpy188III TCNNGA 1 cut(s) 248
Hpy99I CGWCG 3 cut(s) 152, 155, 209
HpyAV CCTTC 1 cut(s) 187
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4V TGCA 2 cut(s) 66, 87
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 221, 294
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 2 cut(s) 70, 244
HspAI GCGC 5 cut(s) 19, 80, 123, 139, 344
KasI GGCGCC 1 cut(s) 343
KpnI GGTACC 1 cut(s) 354
Kzo9I GATC 2 cut(s) 27, 165
LmnI GCTCC 2 cut(s) 274, 328
Lsp1109I GCAGC 1 cut(s) 107
LweI GCATC 1 cut(s) 252
MaeI CTAG 1 cut(s) 360
MaeIII GTNAC 1 cut(s) 172
MalI GATC 2 cut(s) 29, 167
MboI GATC 2 cut(s) 27, 165
MboII GAAGA 2 cut(s) 193, 383
MhlI GDGCHC 1 cut(s) 333
MluI ACGCGT 1 cut(s) 39
Mly113I GGCGCC 1 cut(s) 344
MlyI GAGTC 3 cut(s) 116, 198, 284
MmeI TCCRAC 2 cut(s) 24, 239
MnlI CCTC 6 cut(s) 18, 23, 187, 190, 292, 319
MspI CCGG 5 cut(s) 117, 294, 308, 320, 341
MspR9I CCNGG 3 cut(s) 117, 304, 348
Mva1269I GAATGC 1 cut(s) 231
MvaI CCWGG 2 cut(s) 304, 348
MvnI CGCG 5 cut(s) 19, 21, 41, 209, 288
MwoI GCNNNNNNNGC 3 cut(s) 98, 221, 294
NarI GGCGCC 1 cut(s) 344
NciI CCSGG 1 cut(s) 117
NdeII GATC 2 cut(s) 27, 165
NlaIII CATG 2 cut(s) 70, 244
NlaIV GGNNCC 3 cut(s) 48, 345, 352
NmeAIII GCCGAG 1 cut(s) 349
NmuCI GTSAC 1 cut(s) 172
PceI AGGCCT 1 cut(s) 302
PctI GAATGC 1 cut(s) 231
PfeI GAWTC 1 cut(s) 244
PkrI GCNGC 2 cut(s) 122, 143
PleI GAGTC 3 cut(s) 115, 197, 284
PluTI GGCGCC 1 cut(s) 347
PpsI GAGTC 3 cut(s) 115, 197, 284
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 2 cut(s) 302, 346
PspGI CCWGG 2 cut(s) 302, 346
PspN4I GGNNCC 3 cut(s) 48, 345, 352
PspPI GGNCC 2 cut(s) 46, 237
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
SacI GAGCTC 1 cut(s) 333
SatI GCNGC 2 cut(s) 121, 142
Sau3AI GATC 2 cut(s) 27, 165
Sau96I GGNCC 2 cut(s) 46, 237
SchI GAGTC 3 cut(s) 116, 198, 284
ScrFI CCNGG 3 cut(s) 117, 304, 348
SduI GDGCHC 1 cut(s) 333
SetI ASST 3 cut(s) 179, 201, 333
SfaNI GCATC 1 cut(s) 252
SfoI GGCGCC 1 cut(s) 345
SinI GGWCC 2 cut(s) 46, 237
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
SseBI AGGCCT 1 cut(s) 302
SsiI CCGC 3 cut(s) 142, 213, 286
SspDI GGCGCC 1 cut(s) 343
SspMI CTAG 1 cut(s) 360
SstI GAGCTC 1 cut(s) 333
StuI AGGCCT 1 cut(s) 302
StyD4I CCNGG 3 cut(s) 115, 302, 346
StyI CCWWGG 2 cut(s) 96, 359
TaaI ACNGT 1 cut(s) 172
TaqI TCGA 4 cut(s) 30, 192, 204, 249
TauI GCSGC 1 cut(s) 144
TfiI GAWTC 1 cut(s) 244
TscAI CASTG 1 cut(s) 89
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 1 cut(s) 120
Tsp45I GTSAC 1 cut(s) 172
TspGWI ACGGA 2 cut(s) 201, 287
TspRI CASTG 1 cut(s) 89
VpaK11BI GGWCC 2 cut(s) 46, 237
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XmaJI CCTAGG 1 cut(s) 359
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.