AT5G44550

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012085
Physical Location & Seq
Reverse (-)
17941842 .. 17943283
1442 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G44550.1

Sequence Viewer

Length: 594 bp
ATGGCGGTTTCAAAGCTTACATTGGCTGCTACGAGCGGTAAGAGCTGCAAAATACTCTTAGGGCTGAGATTACTTGCCTTCTCAGCCACGTTATCTGCAGCTATTGTAATGGGACTCAACAAAGAGACAAAGACTTTTATTGTGGGCAAAGTTGGAAATACTCCAATTCAAGCTACTTTTACTGCTAAGTTCGACCACACACCAGCTTTTGTGTTTTTCGTAGTAGCTAATGCAATGGTGAGCTTCCACAACTTGTTGATGATTGCTCTTCAGATATTTGGAGGGAAAATGGAGTTCACTGGTTTTCGGCTTCTCTCAGTCGCCATTCTTGACATGCTAAATGTGACTCTAATTTCGGCGGCAGCAAACGCGGCGGCGTTCATGGCGGAGGTAGGAAAGAACGGGAACAAACACGCGAGATGGGACAAAATCTGTGATAGATTCGCCACTTACTGTGATCACGGCGCCGGAGCATTAATCGCCGCATTCGCCGGCGTAATCCTTATGCTCATCATCTCCGCCGCGTCAATCTCTCGTCTCGTCCAACCTAATAAATGCTGCTCCACCACCGCATCTCCCTCGGTCGTCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

197

Amino Acids

20.75

Weight (kDa)

9.64

Isoelectric Point (pI)

28.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 16 - 168 3e-40 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20390 AT5G44550
fragaria_vesca FvH4_3g11410
malus_domestica MD05G1263600.v1.1 MD10G1241300.v1.1
prunus_persica Prupe.4G101900_v2.0.a1
pyrus_communis pycom05g24760 pycom10g20230
rosa_chinensis RchiOBHm_Chr5g0018351
rosa_laevigata RLG00000032354
rosa_multiflora Rmu_sc0019610.1_g000001 Rmu_sc0029003.1_g000002
rosa_roxburghii Rroxscaffold_1G00059390
rosa_rugosa Rorug05G0041700.1
rosa_samantha Rh5AG135000 Rh5BG133600 Rh5CG145400 Rh5DG135000
rosa_wichuraiana Rw5G011790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 464
AccBSI CCGCTC 1 cut(s) 36
AccII CGCG 3 cut(s) 371, 416, 524
AcuI CTGAAG 1 cut(s) 254
AcyI GRCGYC 1 cut(s) 465
AgsI TTSAA 2 cut(s) 12, 170
AluBI AGCT 7 cut(s) 16, 45, 101, 173, 206, 227, 243
AluI AGCT 7 cut(s) 16, 45, 101, 173, 206, 227, 243
Alw26I GTCTC 2 cut(s) 119, 542
ApeKI GCWGC 5 cut(s) 26, 45, 98, 362, 558
ArsI GACNNNNNNTTYG 2 cut(s) 337, 369
AseI ATTAAT 1 cut(s) 476
AspLEI GCGC 1 cut(s) 467
AsuHPI GGTGA 1 cut(s) 250
BanI GGYRCC 1 cut(s) 464
BbvI GCAGC 5 cut(s) 13, 32, 110, 374, 545
BccI CCATC 1 cut(s) 414
BceAI ACGGC 1 cut(s) 478
BcgI CGANNNNNNTGC 1 cut(s) 561
BclI TGATCA 1 cut(s) 457
BcoDI GTCTC 2 cut(s) 119, 542
BfmI CTRYAG 1 cut(s) 96
BfoI RGCGCY 1 cut(s) 468
BmiI GGNNCC 1 cut(s) 466
BmsI GCATC 1 cut(s) 581
BsaHI GRCGYC 1 cut(s) 465
BsaJI CCNNGG 1 cut(s) 579
BsaXI ACNNNNNCTCC 2 cut(s) 559, 589
Bse118I RCCGGY 1 cut(s) 491
Bse1I ACTGG 1 cut(s) 304
Bse3DI GCAATG 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 579
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 3 cut(s) 56, 96, 330
BseNI ACTGG 1 cut(s) 304
BseXI GCAGC 5 cut(s) 13, 32, 110, 374, 545
Bsh1236I CGCG 3 cut(s) 371, 416, 524
Bsh1285I CGRYCG 1 cut(s) 585
BshNI GGYRCC 1 cut(s) 464
BsiEI CGRYCG 1 cut(s) 585
BsiSI CCGG 2 cut(s) 468, 492
BslFI GGGAC 3 cut(s) 126, 437, 572
BsmAI GTCTC 2 cut(s) 119, 542
BsmBI CGTCTC 1 cut(s) 542
BsmFI GGGAC 3 cut(s) 126, 437, 572
BsmI GAATGC 1 cut(s) 485
Bsp143I GATC 1 cut(s) 457
BspCNI CTCAG 3 cut(s) 57, 95, 329
BspFNI CGCG 3 cut(s) 371, 416, 524
BspLI GGNNCC 1 cut(s) 466
BspMAI CTGCAG 1 cut(s) 100
BspQI GCTCTTC 1 cut(s) 273
BspT107I GGYRCC 1 cut(s) 464
BsrBI CCGCTC 1 cut(s) 36
BsrDI GCAATG 1 cut(s) 240
BsrFI RCCGGY 1 cut(s) 491
BsrI ACTGG 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 491
BssECI CCNNGG 1 cut(s) 579
BssMI GATC 1 cut(s) 457
BssNI GRCGYC 1 cut(s) 465
Bst4CI ACNGT 1 cut(s) 455
Bst6I CTCTTC 1 cut(s) 273
BstACI GRCGYC 1 cut(s) 465
BstC8I GCNNGC 1 cut(s) 493
BstDEI CTNAG 5 cut(s) 58, 65, 82, 186, 316
BstFNI CGCG 3 cut(s) 371, 416, 524
BstH2I RGCGCY 1 cut(s) 468
BstHHI GCGC 1 cut(s) 467
BstKTI GATC 1 cut(s) 460
BstMAI GTCTC 2 cut(s) 119, 542
BstMBI GATC 1 cut(s) 457
BstMCI CGRYCG 1 cut(s) 585
BstMWI GCNNNNNNNGC 7 cut(s) 42, 83, 368, 371, 383, 479, 488
BstNSI RCATGY 1 cut(s) 337
BstSFI CTRYAG 1 cut(s) 96
BstUI CGCG 3 cut(s) 371, 416, 524
BstV1I GCAGC 5 cut(s) 13, 32, 110, 374, 545
BtsIMutI CAGTG 1 cut(s) 297
Cac8I GCNNGC 1 cut(s) 493
CfoI GCGC 1 cut(s) 467
Cfr10I RCCGGY 1 cut(s) 491
CseI GACGC 1 cut(s) 513
CviAII CATG 2 cut(s) 334, 382
DdeI CTNAG 5 cut(s) 58, 65, 82, 186, 316
DinI GGCGCC 1 cut(s) 466
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
EciI GGCGGA 2 cut(s) 401, 508
Eco57I CTGAAG 1 cut(s) 254
EgeI GGCGCC 1 cut(s) 466
EheI GGCGCC 1 cut(s) 466
Esp3I CGTCTC 1 cut(s) 542
FaeI CATG 2 cut(s) 337, 385
FaiI YATR 3 cut(s) 335, 383, 506
FaqI GGGAC 3 cut(s) 126, 437, 572
FatI CATG 2 cut(s) 333, 381
FbaI TGATCA 1 cut(s) 457
GlaI GCGC 1 cut(s) 466
HaeII RGCGCY 1 cut(s) 468
HapII CCGG 2 cut(s) 468, 492
HgaI GACGC 1 cut(s) 513
HhaI GCGC 1 cut(s) 467
Hin1I GRCGYC 1 cut(s) 465
Hin1II CATG 2 cut(s) 337, 385
Hin6I GCGC 1 cut(s) 465
HinP1I GCGC 1 cut(s) 465
HindIII AAGCTT 1 cut(s) 14
HinfI GANTC 3 cut(s) 114, 346, 441
HpaII CCGG 2 cut(s) 468, 492
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 1 cut(s) 297
Hpy188I TCNGA 1 cut(s) 273
Hpy188III TCNNGA 1 cut(s) 329
Hpy8I GTNNAC 1 cut(s) 297
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 1 cut(s) 455
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 3 cut(s) 48, 98, 233
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 83, 368, 371, 383, 479, 488
HpyF3I CTNAG 5 cut(s) 58, 65, 82, 186, 316
HpySE526I ACGT 1 cut(s) 89
Hsp92I GRCGYC 1 cut(s) 465
Hsp92II CATG 2 cut(s) 337, 385
HspAI GCGC 1 cut(s) 465
KasI GGCGCC 1 cut(s) 464
KroI GCCGGC 1 cut(s) 491
KroNI GCCGGC 1 cut(s) 493
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 1 cut(s) 457
LguI GCTCTTC 1 cut(s) 273
LmnI GCTCC 2 cut(s) 470, 566
LpnPI CCDG 4 cut(s) 216, 285, 481, 505
Lsp1109I GCAGC 5 cut(s) 13, 32, 110, 374, 545
LweI GCATC 1 cut(s) 581
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 1 cut(s) 343
MalI GATC 1 cut(s) 459
MbiI CCGCTC 1 cut(s) 36
MboI GATC 1 cut(s) 457
MboII GAAGA 1 cut(s) 260
MluCI AATT 2 cut(s) 165, 351
Mly113I GGCGCC 1 cut(s) 465
MlyI GAGTC 2 cut(s) 108, 340
MmeI TCCRAC 2 cut(s) 133, 568
MnlI CCTC 3 cut(s) 275, 382, 589
MreI CGCCGGCG 1 cut(s) 491
MroNI GCCGGC 1 cut(s) 491
MseI TTAA 1 cut(s) 476
MspI CCGG 2 cut(s) 468, 492
Mva1269I GAATGC 1 cut(s) 485
MvnI CGCG 3 cut(s) 371, 416, 524
MwoI GCNNNNNNNGC 7 cut(s) 42, 83, 368, 371, 383, 479, 488
NaeI GCCGGC 1 cut(s) 493
NarI GGCGCC 1 cut(s) 465
NdeII GATC 1 cut(s) 457
NgoMIV GCCGGC 1 cut(s) 491
NlaIII CATG 2 cut(s) 337, 385
NlaIV GGNNCC 1 cut(s) 466
NmuCI GTSAC 1 cut(s) 343
NspI RCATGY 1 cut(s) 337
PciSI GCTCTTC 1 cut(s) 273
PctI GAATGC 1 cut(s) 485
PdiI GCCGGC 1 cut(s) 493
PfeI GAWTC 1 cut(s) 441
PleI GAGTC 2 cut(s) 108, 340
PluTI GGCGCC 1 cut(s) 468
PpsI GAGTC 2 cut(s) 108, 340
PshBI ATTAAT 1 cut(s) 476
PspN4I GGNNCC 1 cut(s) 466
PstI CTGCAG 1 cut(s) 100
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 1 cut(s) 476
Sau3AI GATC 1 cut(s) 457
SchI GAGTC 2 cut(s) 108, 340
SfaNI GCATC 1 cut(s) 581
SfcI CTRYAG 1 cut(s) 96
SfoI GGCGCC 1 cut(s) 466
SgrAI CRCCGGYG 1 cut(s) 491
Sse9I AATT 2 cut(s) 165, 351
SspDI GGCGCC 1 cut(s) 464
TaaI ACNGT 1 cut(s) 455
TaiI ACGT 1 cut(s) 92
TaqI TCGA 1 cut(s) 192
TaqII GACCGA 1 cut(s) 571
TasI AATT 2 cut(s) 165, 351
TauI GCSGC 5 cut(s) 362, 374, 377, 485, 524
TfiI GAWTC 1 cut(s) 441
Tru1I TTAA 1 cut(s) 476
Tru9I TTAA 1 cut(s) 476
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 1 cut(s) 343
TseI GCWGC 5 cut(s) 26, 45, 98, 362, 558
Tsp45I GTSAC 1 cut(s) 343
TspDTI ATGAA 1 cut(s) 370
TspRI CASTG 1 cut(s) 304
VspI ATTAAT 1 cut(s) 476
XceI RCATGY 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.