MD10G1241300.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012085
Physical Location & Seq
Forward (+)
33674835 .. 33675905
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1241300.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGCTCTAGAAAATGCGCAGAAACTCGAGAACGGGACGGAATTAGTGGCGGTGAGAAAGGTGACGGACTCGACTATTGTCGTACTGAGGGTGGTTGCGTTTTTGGCCACGGTTTCTGCAACCGTTGTGATGGCAATGAACAAAGAAACCAAGTCCATTGTGGTTGCCACTGTTGGGACTACTCCGATAAGCGTCACTCTCACAGCCAAGTTTCAACACACTCCTGCAAATGTGTACTTTGTGATAGCTAATGGAATTGCCAGCTTTCATAACTTGTTCATGATGGCTGTGCACTTTTTTGGAGACCATACGTTCAAGGACAAGGGCCTTCAGCTTGTAATGGTGGCCATTTTAGACGTGATGATTGTGGCCCTATTATCTTCAGGCGATGGTGCAGCAACCTTCATGGCACAGCTAGGGAAGAAGGGAAATTCGCACGCAATGTGGAACAAAATCTGTGACAAGTTCGAGAAGTTCTGCGACCATGCAGGTGGTGCCATCATCGCCTCCTACATCGGGCTCCTTCTCTTGTTGGTTATCACAGCGTTGTCCATCATCAAACTACTCAAAAGAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

193

Amino Acids

20.66

Weight (kDa)

9.3

Isoelectric Point (pI)

16.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 23 - 175 6.5e-37 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20390 AT5G44550
fragaria_vesca FvH4_3g11410
malus_domestica MD05G1263600.v1.1 MD10G1241300.v1.1
prunus_persica Prupe.4G101900_v2.0.a1
pyrus_communis pycom05g24760 pycom10g20230
rosa_chinensis RchiOBHm_Chr5g0018351
rosa_laevigata RLG00000032354
rosa_multiflora Rmu_sc0019610.1_g000001 Rmu_sc0029003.1_g000002
rosa_roxburghii Rroxscaffold_1G00059390
rosa_rugosa Rorug05G0041700.1
rosa_samantha Rh5AG135000 Rh5BG133600 Rh5CG145400 Rh5DG135000
rosa_wichuraiana Rw5G011790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 479
Acc16I TGCGCA 1 cut(s) 18
Acc36I ACCTGC 1 cut(s) 479
AccB1I GGYRCC 1 cut(s) 494
AciI CCGC 1 cut(s) 50
AcoI YGGCCR 2 cut(s) 105, 345
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 2 cut(s) 314, 366
AfaI GTAC 2 cut(s) 84, 236
AfiI CCNNNNNNNGG 2 cut(s) 174, 516
AgsI TTSAA 2 cut(s) 215, 316
AjiI CACGTC 1 cut(s) 358
AluBI AGCT 4 cut(s) 248, 264, 334, 415
AluI AGCT 4 cut(s) 248, 264, 334, 415
Alw21I GWGCWC 1 cut(s) 294
Alw26I GTCTC 1 cut(s) 297
Alw44I GTGCAC 1 cut(s) 290
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 4 cut(s) 105, 325, 345, 369
ApaLI GTGCAC 1 cut(s) 290
ApeKI GCWGC 1 cut(s) 395
ApoI RAATTY 1 cut(s) 430
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 2 cut(s) 325, 370
AsuHPI GGTGA 2 cut(s) 64, 73
AvaI CYCGRG 1 cut(s) 26
BaeGI GKGCMC 1 cut(s) 294
BalI TGGCCA 2 cut(s) 107, 347
BanI GGYRCC 1 cut(s) 494
BanII GRGCYC 1 cut(s) 522
Bbv12I GWGCWC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 407
BccI CCATC 5 cut(s) 124, 277, 383, 506, 560
BcoDI GTCTC 1 cut(s) 297
BfaI CTAG 2 cut(s) 8, 416
BfuAI ACCTGC 1 cut(s) 479
BisI GCNGC 1 cut(s) 396
BlsI GCNGC 1 cut(s) 397
BmeT110I CYCGRG 1 cut(s) 26
BmgBI CACGTC 1 cut(s) 358
BmgT120I GGNCC 2 cut(s) 325, 370
BmiI GGNNCC 2 cut(s) 496, 521
BoxI GACNNNNGTC 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 108
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 516
Bse3DI GCAATG 2 cut(s) 141, 447
BseDI CCNNGG 1 cut(s) 108
BseLI CCNNNNNNNGG 2 cut(s) 174, 516
BseMI GCAATG 2 cut(s) 141, 447
BseMII CTCAG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 294
BseXI GCAGC 1 cut(s) 407
BsgI GTGCAG 1 cut(s) 414
BshFI GGCC 4 cut(s) 107, 327, 347, 371
BshNI GGYRCC 1 cut(s) 494
BsiHKAI GWGCWC 1 cut(s) 294
BsiHKCI CYCGRG 1 cut(s) 26
BslFI GGGAC 2 cut(s) 49, 190
BslI CCNNNNNNNGG 2 cut(s) 174, 516
BsmAI GTCTC 1 cut(s) 297
BsmFI GGGAC 2 cut(s) 49, 190
BsnI GGCC 4 cut(s) 107, 327, 347, 371
Bso31I GGTCTC 1 cut(s) 297
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 294, 522
BspACI CCGC 1 cut(s) 50
BspANI GGCC 4 cut(s) 107, 327, 347, 371
BspCNI CTCAG 1 cut(s) 78
BspHI TCATGA 1 cut(s) 279
BspLI GGNNCC 2 cut(s) 496, 521
BspMI ACCTGC 1 cut(s) 479
BspT107I GGYRCC 1 cut(s) 494
BspTNI GGTCTC 1 cut(s) 297
BsrDI GCAATG 2 cut(s) 141, 447
BssECI CCNNGG 1 cut(s) 108
Bst4CI ACNGT 3 cut(s) 112, 124, 172
BstAPI GCANNNNNTGC 1 cut(s) 494
BstC8I GCNNGC 2 cut(s) 262, 438
BstDEI CTNAG 1 cut(s) 86
BstDSI CCRYGG 1 cut(s) 108
BstHHI GCGC 1 cut(s) 19
BstMAI GTCTC 1 cut(s) 297
BstMWI GCNNNNNNNGC 3 cut(s) 104, 494, 503
BstPAI GACNNNNGTC 1 cut(s) 77
BstSLI GKGCMC 1 cut(s) 294
BstV1I GCAGC 1 cut(s) 407
BstXI CCANNNNNNTGG 1 cut(s) 491
BsuRI GGCC 4 cut(s) 107, 327, 347, 371
BtgI CCRYGG 1 cut(s) 108
BtgZI GCGATG 2 cut(s) 402, 487
BtrI CACGTC 1 cut(s) 358
BtsIMutI CAGTG 1 cut(s) 168
BveI ACCTGC 1 cut(s) 479
Cac8I GCNNGC 2 cut(s) 262, 438
CciI TCATGA 1 cut(s) 279
CfoI GCGC 1 cut(s) 19
Cfr13I GGNCC 2 cut(s) 325, 370
CseI GACGC 1 cut(s) 181
Csp6I GTAC 2 cut(s) 83, 235
CviAII CATG 3 cut(s) 280, 406, 485
CviQI GTAC 2 cut(s) 83, 235
DdeI CTNAG 1 cut(s) 86
EaeI YGGCCR 2 cut(s) 105, 345
Eco24I GRGCYC 1 cut(s) 522
Eco31I GGTCTC 1 cut(s) 297
Eco57I CTGAAG 2 cut(s) 314, 366
Eco88I CYCGRG 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 325
EcoT38I GRGCYC 1 cut(s) 522
FaeI CATG 3 cut(s) 283, 409, 488
FaiI YATR 5 cut(s) 270, 281, 309, 407, 486
FaqI GGGAC 2 cut(s) 49, 190
FatI CATG 3 cut(s) 279, 405, 484
Fnu4HI GCNGC 1 cut(s) 396
FriOI GRGCYC 1 cut(s) 522
Fsp4HI GCNGC 1 cut(s) 396
FspBI CTAG 2 cut(s) 8, 416
FspI TGCGCA 1 cut(s) 18
GlaI GCGC 1 cut(s) 18
GluI GCNGC 1 cut(s) 396
HaeIII GGCC 4 cut(s) 107, 327, 347, 371
HgaI GACGC 1 cut(s) 181
HhaI GCGC 1 cut(s) 19
Hin1II CATG 3 cut(s) 283, 409, 488
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HinfI GANTC 1 cut(s) 68
HphI GGTGA 2 cut(s) 64, 73
Hpy166II GTNNAC 2 cut(s) 235, 292
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 4 cut(s) 8, 28, 280, 469
Hpy8I GTNNAC 2 cut(s) 235, 292
HpyAV CCTTC 4 cut(s) 338, 412, 418, 533
HpyCH4III ACNGT 3 cut(s) 112, 124, 172
HpyCH4IV ACGT 2 cut(s) 311, 357
HpyCH4V TGCA 5 cut(s) 119, 227, 292, 395, 488
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 494, 503
HpyF3I CTNAG 1 cut(s) 86
HpySE526I ACGT 2 cut(s) 311, 357
Hsp92II CATG 3 cut(s) 283, 409, 488
HspAI GCGC 1 cut(s) 17
LmnI GCTCC 1 cut(s) 525
LpnPI CCDG 4 cut(s) 237, 274, 369, 474
Lsp1109I GCAGC 1 cut(s) 407
MaeI CTAG 2 cut(s) 8, 416
MaeII ACGT 2 cut(s) 311, 357
MaeIII GTNAC 3 cut(s) 61, 193, 458
MboII GAAGA 2 cut(s) 372, 433
MhlI GDGCHC 2 cut(s) 294, 522
MlsI TGGCCA 2 cut(s) 107, 347
MluCI AATT 4 cut(s) 41, 255, 430, 574
MluNI TGGCCA 2 cut(s) 107, 347
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 2 cut(s) 81, 517
Mox20I TGGCCA 2 cut(s) 107, 347
MscI TGGCCA 2 cut(s) 107, 347
MslI CAYNNNNRTG 1 cut(s) 489
Msp20I TGGCCA 2 cut(s) 107, 347
MwoI GCNNNNNNNGC 3 cut(s) 104, 494, 503
NlaIII CATG 3 cut(s) 283, 409, 488
NlaIV GGNNCC 2 cut(s) 496, 521
NmuCI GTSAC 3 cut(s) 61, 193, 458
NsbI TGCGCA 1 cut(s) 18
PaeR7I CTCGAG 1 cut(s) 26
PagI TCATGA 1 cut(s) 279
PaqCI CACCTGC 1 cut(s) 479
PkrI GCNGC 1 cut(s) 397
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
PshAI GACNNNNGTC 1 cut(s) 77
PspN4I GGNNCC 2 cut(s) 496, 521
PspPI GGNCC 2 cut(s) 325, 370
RsaI GTAC 2 cut(s) 84, 236
RsaNI GTAC 2 cut(s) 83, 235
RseI CAYNNNNRTG 1 cut(s) 489
SatI GCNGC 1 cut(s) 396
Sau96I GGNCC 2 cut(s) 325, 370
SchI GAGTC 1 cut(s) 62
SduI GDGCHC 2 cut(s) 294, 522
SetI ASST 9 cut(s) 63, 250, 266, 314, 336, 360, 404, 417, 493
Sfr274I CTCGAG 1 cut(s) 26
SlaI CTCGAG 1 cut(s) 26
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 4 cut(s) 41, 255, 430, 574
SsiI CCGC 1 cut(s) 50
SspMI CTAG 2 cut(s) 8, 416
TaaI ACNGT 3 cut(s) 112, 124, 172
TaiI ACGT 2 cut(s) 314, 360
TaqI TCGA 3 cut(s) 27, 71, 468
TasI AATT 4 cut(s) 41, 255, 430, 574
TatI WGTACW 1 cut(s) 234
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 3 cut(s) 61, 193, 458
TseI GCWGC 1 cut(s) 395
Tsp45I GTSAC 3 cut(s) 61, 193, 458
TspDTI ATGAA 4 cut(s) 152, 257, 268, 394
TspGWI ACGGA 2 cut(s) 53, 80
TspRI CASTG 1 cut(s) 175
VneI GTGCAC 1 cut(s) 290
XapI RAATTY 1 cut(s) 430
XbaI TCTAGA 1 cut(s) 7
XcmI CCANNNNNNNNNTGG 1 cut(s) 157
XhoI CTCGAG 1 cut(s) 26
XspI CTAG 2 cut(s) 8, 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.