RLG00000032354

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012085
Physical Location & Seq
Reverse (-)
13891822 .. 13892627
806 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032354

Sequence Viewer

Length: 570 bp
ATGGCGCTAGAAAATGGAGAGAAACCAGGGTCTAGTTTCGTGGCAGTGAGAAAACCAAATGATTGTACCATTCTCTTGCTGAGAGTGGTTGCATTCTTGGCCACGCTTGCTGCAACTCTTGTGATGGCACTTAACAAAGAAACCAAAACCTTGGTTGTTGCCACTATTGGGACTACTCCAATTAAAGCCACTCTGACAGCCAAGTTTCAACACACTCCAGCAAATGTGTTCTTTGTTGTAGCTAATGCAATGGCCAGCCTTCACAACTTGTCAATGATAATAGTGCAACTTTGCGGAGGCAAGTTCAAAGACAAGGGTCTTCGCCTTGTGATGATGGCCATTTTAGACATGATGACTGTGGCCCTGCTATCTTCTGGAGATGGTGCAGCTACCTTCATGGCACATCTAGCGAAGAAAGGTAATTCGCATGCAAGGTGGGACAAAATCTGTGACAAGTTTGGCAAGTTCTGCGACCATGGTGGCGGCGCTCTCATTGCTTCTTATATTGGGCTCCTTCTCTTGCTGGTTATCACAGTGTTGTCCATCATCAAGCTACTCAAGACGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

190

Amino Acids

20.16

Weight (kDa)

9.76

Isoelectric Point (pI)

19.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 21 - 172 2.3e-37 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20390 AT5G44550
fragaria_vesca FvH4_3g11410
malus_domestica MD05G1263600.v1.1 MD10G1241300.v1.1
prunus_persica Prupe.4G101900_v2.0.a1
pyrus_communis pycom05g24760 pycom10g20230
rosa_chinensis RchiOBHm_Chr5g0018351
rosa_laevigata RLG00000032354
rosa_multiflora Rmu_sc0019610.1_g000001 Rmu_sc0029003.1_g000002
rosa_roxburghii Rroxscaffold_1G00059390
rosa_rugosa Rorug05G0041700.1
rosa_samantha Rh5AG135000 Rh5BG133600 Rh5CG145400 Rh5DG135000
rosa_wichuraiana Rw5G011790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 294, 483
AcoI YGGCCR 3 cut(s) 99, 252, 336
AfaI GTAC 1 cut(s) 67
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 2 cut(s) 209, 307
AjnI CCWGG 1 cut(s) 25
AluBI AGCT 3 cut(s) 242, 389, 553
AluI AGCT 3 cut(s) 242, 389, 553
AoxI GGCC 4 cut(s) 99, 252, 336, 360
ApeKI GCWGC 2 cut(s) 110, 386
AspLEI GCGC 2 cut(s) 7, 488
AspS9I GGNCC 1 cut(s) 361
BalI TGGCCA 3 cut(s) 101, 254, 338
BanII GRGCYC 1 cut(s) 513
BbsI GAAGAC 1 cut(s) 311
BbvI GCAGC 2 cut(s) 97, 398
BccI CCATC 4 cut(s) 118, 328, 374, 551
BciT130I CCWGG 1 cut(s) 27
BfaI CTAG 3 cut(s) 8, 33, 407
BfoI RGCGCY 2 cut(s) 8, 489
BisI GCNGC 3 cut(s) 111, 387, 484
BlsI GCNGC 3 cut(s) 112, 388, 485
Bme1390I CCNGG 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 361
BmiI GGNNCC 1 cut(s) 512
BmrFI CCNGG 1 cut(s) 27
BoxI GACNNNNGTC 1 cut(s) 315
BpiI GAAGAC 1 cut(s) 311
BpmI CTGGAG 2 cut(s) 201, 396
BpuEI CTTGAG 1 cut(s) 542
BsaJI CCNNGG 3 cut(s) 26, 150, 475
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse3DI GCAATG 2 cut(s) 255, 492
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 3 cut(s) 26, 150, 475
BseLI CCNNNNNNNGG 1 cut(s) 168
BseMI GCAATG 2 cut(s) 255, 492
BseMII CTCAG 1 cut(s) 71
BseXI GCAGC 2 cut(s) 97, 398
BsgI GTGCAG 1 cut(s) 405
BshFI GGCC 4 cut(s) 101, 254, 338, 362
BslFI GGGAC 2 cut(s) 184, 452
BslI CCNNNNNNNGG 1 cut(s) 168
BsmFI GGGAC 2 cut(s) 184, 452
BsmI GAATGC 1 cut(s) 92
BsnI GGCC 4 cut(s) 101, 254, 338, 362
Bsp1286I GDGCHC 1 cut(s) 513
Bsp19I CCATGG 1 cut(s) 475
BspACI CCGC 2 cut(s) 294, 483
BspANI GGCC 4 cut(s) 101, 254, 338, 362
BspCNI CTCAG 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 512
BsrDI GCAATG 2 cut(s) 255, 492
BssECI CCNNGG 3 cut(s) 26, 150, 475
BssT1I CCWWGG 2 cut(s) 150, 475
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 2 cut(s) 358, 535
BstAPI GCANNNNNTGC 1 cut(s) 468
BstC8I GCNNGC 3 cut(s) 108, 256, 429
BstDEI CTNAG 1 cut(s) 80
BstDSI CCRYGG 1 cut(s) 475
BstH2I RGCGCY 2 cut(s) 8, 489
BstHHI GCGC 2 cut(s) 7, 488
BstMWI GCNNNNNNNGC 5 cut(s) 98, 107, 407, 468, 494
BstNI CCWGG 1 cut(s) 27
BstNSI RCATGY 1 cut(s) 431
BstPAI GACNNNNGTC 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 25
BstV1I GCAGC 2 cut(s) 97, 398
BstV2I GAAGAC 1 cut(s) 311
BstXI CCANNNNNNTGG 1 cut(s) 151
BsuRI GGCC 4 cut(s) 101, 254, 338, 362
BtgI CCRYGG 1 cut(s) 475
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 2 cut(s) 51, 540
Cac8I GCNNGC 3 cut(s) 108, 256, 429
CfoI GCGC 2 cut(s) 7, 488
Cfr13I GGNCC 1 cut(s) 361
Csp6I GTAC 1 cut(s) 66
CviAII CATG 4 cut(s) 349, 397, 428, 476
CviQI GTAC 1 cut(s) 66
DdeI CTNAG 1 cut(s) 80
EaeI YGGCCR 3 cut(s) 99, 252, 336
Eco130I CCWWGG 2 cut(s) 150, 475
Eco24I GRGCYC 1 cut(s) 513
EcoRII CCWGG 1 cut(s) 25
EcoT14I CCWWGG 2 cut(s) 150, 475
EcoT38I GRGCYC 1 cut(s) 513
ErhI CCWWGG 2 cut(s) 150, 475
FaeI CATG 4 cut(s) 352, 400, 431, 479
FaiI YATR 5 cut(s) 350, 398, 429, 477, 504
FaqI GGGAC 2 cut(s) 184, 452
FatI CATG 4 cut(s) 348, 396, 427, 475
Fnu4HI GCNGC 3 cut(s) 111, 387, 484
FriOI GRGCYC 1 cut(s) 513
Fsp4HI GCNGC 3 cut(s) 111, 387, 484
FspBI CTAG 3 cut(s) 8, 33, 407
GlaI GCGC 2 cut(s) 6, 487
GluI GCNGC 3 cut(s) 111, 387, 484
GsuI CTGGAG 2 cut(s) 201, 396
HaeII RGCGCY 2 cut(s) 8, 489
HaeIII GGCC 4 cut(s) 101, 254, 338, 362
HhaI GCGC 2 cut(s) 7, 488
Hin1II CATG 4 cut(s) 352, 400, 431, 479
Hin6I GCGC 2 cut(s) 5, 486
HinP1I GCGC 2 cut(s) 5, 486
Hpy188I TCNGA 1 cut(s) 195
Hpy188III TCNNGA 2 cut(s) 375, 559
HpyAV CCTTC 3 cut(s) 269, 403, 524
HpyCH4III ACNGT 2 cut(s) 358, 535
HpyCH4V TGCA 6 cut(s) 92, 113, 248, 286, 386, 431
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 107, 407, 468, 494
HpyF3I CTNAG 1 cut(s) 80
Hsp92II CATG 4 cut(s) 352, 400, 431, 479
HspAI GCGC 2 cut(s) 5, 486
LmnI GCTCC 1 cut(s) 516
LpnPI CCDG 7 cut(s) 12, 39, 231, 268, 360, 377, 509
Lsp1109I GCAGC 2 cut(s) 97, 398
MaeI CTAG 3 cut(s) 8, 33, 407
MaeIII GTNAC 1 cut(s) 449
MboII GAAGA 3 cut(s) 311, 363, 424
MhlI GDGCHC 1 cut(s) 513
MlsI TGGCCA 3 cut(s) 101, 254, 338
MluCI AATT 2 cut(s) 180, 421
MluNI TGGCCA 3 cut(s) 101, 254, 338
MnlI CCTC 1 cut(s) 290
Mox20I TGGCCA 3 cut(s) 101, 254, 338
MscI TGGCCA 3 cut(s) 101, 254, 338
MseI TTAA 2 cut(s) 132, 183
Msp20I TGGCCA 3 cut(s) 101, 254, 338
MspR9I CCNGG 1 cut(s) 27
Mva1269I GAATGC 1 cut(s) 92
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 5 cut(s) 98, 107, 407, 468, 494
NcoI CCATGG 1 cut(s) 475
NlaIII CATG 4 cut(s) 352, 400, 431, 479
NlaIV GGNNCC 1 cut(s) 512
NmuCI GTSAC 1 cut(s) 449
NspI RCATGY 1 cut(s) 431
PaeI GCATGC 1 cut(s) 431
PctI GAATGC 1 cut(s) 92
PkrI GCNGC 3 cut(s) 112, 388, 485
PshAI GACNNNNGTC 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 25
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 512
PspPI GGNCC 1 cut(s) 361
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
SaqAI TTAA 2 cut(s) 132, 183
SatI GCNGC 3 cut(s) 111, 387, 484
Sau96I GGNCC 1 cut(s) 361
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 1 cut(s) 513
SetI ASST 7 cut(s) 152, 244, 391, 395, 421, 437, 555
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
SphI GCATGC 1 cut(s) 431
Sse9I AATT 2 cut(s) 180, 421
SsiI CCGC 2 cut(s) 294, 483
SspMI CTAG 3 cut(s) 8, 33, 407
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 2 cut(s) 150, 475
TaaI ACNGT 2 cut(s) 358, 535
TasI AATT 2 cut(s) 180, 421
TauI GCSGC 1 cut(s) 486
Tru1I TTAA 2 cut(s) 132, 183
Tru9I TTAA 2 cut(s) 132, 183
TscAI CASTG 2 cut(s) 51, 540
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 2 cut(s) 110, 386
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 1 cut(s) 385
TspRI CASTG 2 cut(s) 51, 540
XceI RCATGY 1 cut(s) 431
XspI CTAG 3 cut(s) 8, 33, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.