MD05G1263600.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012085
Physical Location & Seq
Forward (+)
39899218 .. 39900138
921 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1263600.v1.1.491

Sequence Viewer

Length: 564 bp
ATGGCTCTAGAAAATGGACAGAAACCCGAGGATAGGGCGGAGTTAGCGGCAGTGACGAAGCCGACGGACTGGACTATTGTCGTGCTGAGGGTGGTTGGGTTTTTTGCCACGGTGACTGCAACCGTTGTAATGGCAAGCAACAAAGAAACCAAGTCCTTGGTGGTTGCCCCACCAATAAGCGTCACTCTCACAGCCAAGTTTCAGCACAATCCTGCAAATGTGTACTTTGTGATAGCTAATGGAATTGCAAGCCTTCATAACGTGTTCATGATAGTTGTGCACTTTTTCGGAGACAAGTTCAAGGACAAGGGCCTTCAACTTGTGATGGTAGCCGTTTTAGACATGATGATTGTGGCCCTGGTATCTTCGGGCGATGGTGCAGCAACCTTCATGGCAGAGCTAGGGAAGAAGGGAAACTCGCACGCAAGGTGGAACAAAATCTGTGACAAGTTCGAGAAGTACTGCGACCGTGCCGGCGGTGCCATCATCGCTTCCTACATCGGACTCCTCTTTTTGCTGGTTATCACAGTGTTGTCCATCATCAAACTACTCAAAAGAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

188

Amino Acids

20.33

Weight (kDa)

9.39

Isoelectric Point (pI)

19.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 21 - 170 1.2e-37 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20390 AT5G44550
fragaria_vesca FvH4_3g11410
malus_domestica MD05G1263600.v1.1 MD10G1241300.v1.1
prunus_persica Prupe.4G101900_v2.0.a1
pyrus_communis pycom05g24760 pycom10g20230
rosa_chinensis RchiOBHm_Chr5g0018351
rosa_laevigata RLG00000032354
rosa_multiflora Rmu_sc0019610.1_g000001 Rmu_sc0029003.1_g000002
rosa_roxburghii Rroxscaffold_1G00059390
rosa_rugosa Rorug05G0041700.1
rosa_samantha Rh5AG135000 Rh5BG133600 Rh5CG145400 Rh5DG135000
rosa_wichuraiana Rw5G011790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 479
AciI CCGC 3 cut(s) 38, 47, 477
AfaI GTAC 2 cut(s) 224, 461
AfiI CCNNNNNNNGG 1 cut(s) 33
AflIII ACRYGT 1 cut(s) 261
AgsI TTSAA 2 cut(s) 301, 317
AjnI CCWGG 1 cut(s) 357
AluBI AGCT 2 cut(s) 236, 400
AluI AGCT 2 cut(s) 236, 400
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 1 cut(s) 285
Alw44I GTGCAC 1 cut(s) 278
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 2 cut(s) 310, 354
ApaLI GTGCAC 1 cut(s) 278
ApeKI GCWGC 1 cut(s) 380
AspS9I GGNCC 2 cut(s) 310, 355
AsuHPI GGTGA 1 cut(s) 124
AvaI CYCGRG 1 cut(s) 26
BaeGI GKGCMC 1 cut(s) 282
BanI GGYRCC 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 282
BbvCI CCTCAGC 1 cut(s) 86
BbvI GCAGC 1 cut(s) 392
BccI CCATC 4 cut(s) 319, 368, 491, 545
BceAI ACGGC 1 cut(s) 317
BciT130I CCWGG 1 cut(s) 359
BcoDI GTCTC 1 cut(s) 285
BfaI CTAG 2 cut(s) 8, 401
BisI GCNGC 2 cut(s) 48, 381
BlsI GCNGC 2 cut(s) 49, 382
BmcAI AGTACT 1 cut(s) 461
Bme1390I CCNGG 1 cut(s) 359
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 2 cut(s) 310, 355
BmiI GGNNCC 1 cut(s) 481
BmrFI CCNGG 1 cut(s) 359
BoxI GACNNNNGTC 1 cut(s) 77
Bpu10I CCTNAGC 1 cut(s) 86
BsaJI CCNNGG 4 cut(s) 27, 108, 156, 357
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse118I RCCGGY 1 cut(s) 473
Bse1I ACTGG 1 cut(s) 74
BseBI CCWGG 1 cut(s) 359
BseDI CCNNGG 4 cut(s) 27, 108, 156, 357
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMII CTCAG 1 cut(s) 77
BseNI ACTGG 1 cut(s) 74
BseRI GAGGAG 1 cut(s) 497
BseSI GKGCMC 1 cut(s) 282
BseXI GCAGC 1 cut(s) 392
BsgI GTGCAG 1 cut(s) 399
Bsh1285I CGRYCG 1 cut(s) 469
BshFI GGCC 2 cut(s) 312, 356
BshNI GGYRCC 1 cut(s) 479
BsiEI CGRYCG 1 cut(s) 469
BsiHKAI GWGCWC 1 cut(s) 282
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 1 cut(s) 474
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 1 cut(s) 285
BsnI GGCC 2 cut(s) 312, 356
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 282
BspACI CCGC 3 cut(s) 38, 47, 477
BspANI GGCC 2 cut(s) 312, 356
BspCNI CTCAG 1 cut(s) 78
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 481
BspT107I GGYRCC 1 cut(s) 479
BsrFI RCCGGY 1 cut(s) 473
BsrI ACTGG 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 473
BssECI CCNNGG 4 cut(s) 27, 108, 156, 357
BssT1I CCWWGG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 359
Bst4CI ACNGT 4 cut(s) 112, 124, 470, 529
BstC8I GCNNGC 4 cut(s) 136, 250, 423, 475
BstDEI CTNAG 1 cut(s) 86
BstDSI CCRYGG 1 cut(s) 108
BstMAI GTCTC 1 cut(s) 285
BstMCI CGRYCG 1 cut(s) 469
BstMWI GCNNNNNNNGC 3 cut(s) 44, 479, 488
BstNI CCWGG 1 cut(s) 359
BstPAI GACNNNNGTC 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 357
BstSLI GKGCMC 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 392
BstXI CCANNNNNNTGG 1 cut(s) 157
BsuRI GGCC 2 cut(s) 312, 356
BtgI CCRYGG 1 cut(s) 108
BtgZI GCGATG 2 cut(s) 387, 472
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 2 cut(s) 57, 534
Cac8I GCNNGC 4 cut(s) 136, 250, 423, 475
CciI TCATGA 1 cut(s) 267
Cfr10I RCCGGY 1 cut(s) 473
Cfr13I GGNCC 2 cut(s) 310, 355
CseI GACGC 1 cut(s) 169
Csp6I GTAC 2 cut(s) 223, 460
CviAII CATG 3 cut(s) 268, 343, 391
CviJI RGCY 9 cut(s) 5, 61, 194, 236, 252, 312, 332, 356, 400
CviKI_1 RGCY 9 cut(s) 5, 61, 194, 236, 252, 312, 332, 356, 400
CviQI GTAC 2 cut(s) 223, 460
DdeI CTNAG 1 cut(s) 86
EciI GGCGGA 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 156
Eco88I CYCGRG 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 357
EcoT14I CCWWGG 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 3 cut(s) 271, 346, 394
FaiI YATR 4 cut(s) 258, 269, 344, 392
FatI CATG 3 cut(s) 267, 342, 390
Fnu4HI GCNGC 2 cut(s) 48, 381
Fsp4HI GCNGC 2 cut(s) 48, 381
FspBI CTAG 2 cut(s) 8, 401
GluI GCNGC 2 cut(s) 48, 381
HaeIII GGCC 2 cut(s) 312, 356
HapII CCGG 1 cut(s) 474
HgaI GACGC 1 cut(s) 169
Hin1II CATG 3 cut(s) 271, 346, 394
HinfI GANTC 1 cut(s) 504
HpaII CCGG 1 cut(s) 474
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 2 cut(s) 223, 280
Hpy188I TCNGA 2 cut(s) 290, 503
Hpy188III TCNNGA 3 cut(s) 8, 268, 454
Hpy8I GTNNAC 2 cut(s) 223, 280
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 4 cut(s) 263, 323, 397, 403
HpyCH4III ACNGT 4 cut(s) 112, 124, 470, 529
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 5 cut(s) 119, 215, 248, 280, 380
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 479, 488
HpyF3I CTNAG 1 cut(s) 86
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 3 cut(s) 271, 346, 394
KroI GCCGGC 1 cut(s) 473
KroNI GCCGGC 1 cut(s) 475
LpnPI CCDG 6 cut(s) 55, 225, 344, 371, 487, 503
Lsp1109I GCAGC 1 cut(s) 392
MaeI CTAG 2 cut(s) 8, 401
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 4 cut(s) 52, 112, 181, 443
MboII GAAGA 2 cut(s) 357, 418
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 2 cut(s) 243, 559
MlyI GAGTC 1 cut(s) 498
MnlI CCTC 3 cut(s) 22, 81, 518
MroNI GCCGGC 1 cut(s) 473
MspI CCGG 1 cut(s) 474
MspR9I CCNGG 1 cut(s) 359
MvaI CCWGG 1 cut(s) 359
MwoI GCNNNNNNNGC 3 cut(s) 44, 479, 488
NaeI GCCGGC 1 cut(s) 475
NgoMIV GCCGGC 1 cut(s) 473
NlaIII CATG 3 cut(s) 271, 346, 394
NlaIV GGNNCC 1 cut(s) 481
NmuCI GTSAC 4 cut(s) 52, 112, 181, 443
PagI TCATGA 1 cut(s) 267
PdiI GCCGGC 1 cut(s) 475
PkrI GCNGC 2 cut(s) 49, 382
PleI GAGTC 1 cut(s) 498
PpsI GAGTC 1 cut(s) 498
PshAI GACNNNNGTC 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 357
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 1 cut(s) 481
PspPI GGNCC 2 cut(s) 310, 355
RsaI GTAC 2 cut(s) 224, 461
RsaNI GTAC 2 cut(s) 223, 460
SatI GCNGC 2 cut(s) 48, 381
Sau96I GGNCC 2 cut(s) 310, 355
ScaI AGTACT 1 cut(s) 461
SchI GAGTC 1 cut(s) 498
ScrFI CCNGG 1 cut(s) 359
SduI GDGCHC 1 cut(s) 282
SetI ASST 5 cut(s) 238, 264, 389, 402, 431
Sse9I AATT 2 cut(s) 243, 559
SsiI CCGC 3 cut(s) 38, 47, 477
SspMI CTAG 2 cut(s) 8, 401
StyD4I CCNGG 1 cut(s) 357
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 4 cut(s) 112, 124, 470, 529
TaiI ACGT 1 cut(s) 264
TaqI TCGA 1 cut(s) 453
TasI AATT 2 cut(s) 243, 559
TatI WGTACW 2 cut(s) 222, 459
TauI GCSGC 1 cut(s) 50
TscAI CASTG 2 cut(s) 57, 534
TseFI GTSAC 4 cut(s) 52, 112, 181, 443
TseI GCWGC 1 cut(s) 380
Tsp45I GTSAC 4 cut(s) 52, 112, 181, 443
TspDTI ATGAA 3 cut(s) 245, 256, 379
TspGWI ACGGA 1 cut(s) 80
TspRI CASTG 2 cut(s) 57, 534
VneI GTGCAC 1 cut(s) 278
XbaI TCTAGA 1 cut(s) 7
XcmI CCANNNNNNNNNTGG 1 cut(s) 157
XspI CTAG 2 cut(s) 8, 401
ZrmI AGTACT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.