AT5G49320

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014135
Physical Location & Seq
Reverse (-)
19994994 .. 19996357
1364 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G49320.1

Sequence Viewer

Length: 774 bp
ATGTCGAGAACAAGAGTGCGAGCTGTGACCCATCAAGATCTTGTTCCAAACCCTAAAACCACAGATCTCAGCAGCAAAACCGGTATTTTCATCGCTGTTTTGACAATAATCTTTGGCCTCTCCTGCTTTGTTCTCTGCCTCTATGCCGAAGCCACAAGATCTCAGGCGACATGGGGAAGCAAAACATGCGTGTATAATGGTAGCGGGAAAACGCCGTTGTTGTGTGGTGCGATTGCGTTTGTGGGACTAGCGGTGGCGATGGTGGGCTTCCACATGTACTTACTTATCGCAGTCACCACATCGCCGTCTCTGGTTCTGGTTGAGTGGGATCCTGACTCTGTTCCTGCGAAGAGGCTCACTTTTCAAGCTGCCTTCTTCTTCGTCTCAACATGGGTTTGCTTTGGTGTTGGAGAGGTTCTACTGTTGGTTGCACTTAGTGTGGAATCAGGACATCTAAAGAACTGGTCTAAACCAAAACCTAGTTGTCTAGTAATCCGGCAAGGCTTGTTTTCAGCGGCCGGAGTGTTCTCACTTATAACCGTCTTTCTAGCCACGGGACTTTACCTAACCGCTCTTCAAGCACATCGAATCTCTAAGGATCTTGAAAACACACATAGAGAGATCATCGAGGCATCAGTTCTTTATGCATCTCCTCCAAGATCGCCTACAAATCGGATGGCTACGGTTGCTCGAGAAGGTCTTGCTACTGTTAGAGATGAATCGACGTCTTTGGAATATCTTGTTTCTTTAAAGCAACTTGCACATATAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

257

Amino Acids

27.88

Weight (kDa)

9.08

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 79 - 181 1.4e-17 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49320
fragaria_vesca FvH4_4g19280
malus_domestica MD13G1158800.v1.1 MD16G1159000.v1.1
prunus_persica Prupe.1G125800_v2.0.a1
pyrus_communis pycom13g13570 pycom16g13400
rosa_chinensis RchiOBHm_Chr4g0424271
rosa_laevigata RLG00000007491
rosa_multiflora Rmu_sc0002260.1_g000024
rosa_roxburghii Rroxscaffold_5G00366210
rosa_rugosa Rorug04G0194900
rosa_samantha Rh4AG252200 Rh4BG257700 Rh4CG268300 Rh4DG252000
rosa_wichuraiana Rw4G021750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 536
AatII GACGTC 1 cut(s) 728
AccBSI CCGCTC 1 cut(s) 572
AciI CCGC 4 cut(s) 204, 251, 515, 570
AclWI GGATC 3 cut(s) 323, 336, 606
AcoI YGGCCR 1 cut(s) 516
AcyI GRCGYC 1 cut(s) 725
AdeI CACNNNGTG 1 cut(s) 437
AfaI GTAC 1 cut(s) 278
AflIII ACRYGT 1 cut(s) 273
AgeI ACCGGT 1 cut(s) 80
AgsI TTSAA 3 cut(s) 365, 578, 605
AluBI AGCT 2 cut(s) 23, 368
AluI AGCT 2 cut(s) 23, 368
Alw26I GTCTC 2 cut(s) 312, 388
AlwI GGATC 3 cut(s) 323, 336, 606
Ama87I CYCGRG 1 cut(s) 690
AoxI GGCC 2 cut(s) 115, 516
ApeKI GCWGC 2 cut(s) 72, 368
AsiGI ACCGGT 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 690
BamHI GGATCC 1 cut(s) 328
BbvI GCAGC 2 cut(s) 84, 355
BccI CCATC 3 cut(s) 39, 253, 670
BceAI ACGGC 2 cut(s) 199, 289
BcoDI GTCTC 2 cut(s) 312, 388
BfaI CTAG 4 cut(s) 248, 480, 488, 548
BglII AGATCT 3 cut(s) 37, 64, 158
BisI GCNGC 3 cut(s) 73, 369, 516
BlsI GCNGC 3 cut(s) 74, 370, 517
BmeT110I CYCGRG 1 cut(s) 690
BmiI GGNNCC 1 cut(s) 330
BmsI GCATC 2 cut(s) 641, 656
BsaHI GRCGYC 1 cut(s) 725
BsaJI CCNNGG 1 cut(s) 552
BsaWI WCCGGW 1 cut(s) 80
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 1 cut(s) 467
BseDI CCNNGG 1 cut(s) 552
BseGI GGATG 1 cut(s) 681
BseMII CTCAG 2 cut(s) 82, 176
BseNI ACTGG 1 cut(s) 467
BseRI GAGGAG 1 cut(s) 642
BseX3I CGGCCG 1 cut(s) 516
BseXI GCAGC 2 cut(s) 84, 355
Bsh1285I CGRYCG 1 cut(s) 519
BshFI GGCC 2 cut(s) 117, 518
BshTI ACCGGT 1 cut(s) 80
BsiEI CGRYCG 1 cut(s) 519
BsiHKCI CYCGRG 1 cut(s) 690
BsiSI CCGG 3 cut(s) 81, 496, 519
BslFI GGGAC 2 cut(s) 258, 570
BsmAI GTCTC 2 cut(s) 312, 388
BsmBI CGTCTC 2 cut(s) 312, 388
BsmFI GGGAC 2 cut(s) 258, 570
BsnI GGCC 2 cut(s) 117, 518
BsoBI CYCGRG 1 cut(s) 690
Bsp143I GATC 7 cut(s) 37, 64, 158, 328, 598, 621, 659
BspACI CCGC 4 cut(s) 204, 251, 515, 570
BspANI GGCC 2 cut(s) 117, 518
BspCNI CTCAG 2 cut(s) 81, 175
BspLI GGNNCC 1 cut(s) 330
BspPI GGATC 3 cut(s) 323, 336, 606
BspQI GCTCTTC 1 cut(s) 579
BsrBI CCGCTC 1 cut(s) 572
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 1 cut(s) 467
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 552
BssMI GATC 7 cut(s) 37, 64, 158, 328, 598, 621, 659
BssNI GRCGYC 1 cut(s) 725
Bst4CI ACNGT 4 cut(s) 423, 541, 685, 709
Bst6I CTCTTC 2 cut(s) 344, 579
BstACI GRCGYC 1 cut(s) 725
BstAPI GCANNNNNTGC 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 4 cut(s) 68, 162, 434, 594
BstDSI CCRYGG 1 cut(s) 552
BstF5I GGATG 1 cut(s) 681
BstKTI GATC 7 cut(s) 40, 67, 161, 331, 601, 624, 662
BstMAI GTCTC 2 cut(s) 312, 388
BstMBI GATC 7 cut(s) 37, 64, 158, 328, 598, 621, 659
BstMCI CGRYCG 1 cut(s) 519
BstMWI GCNNNNNNNGC 4 cut(s) 123, 186, 578, 686
BstNSI RCATGY 2 cut(s) 189, 277
BstV1I GCAGC 2 cut(s) 84, 355
BstX2I RGATCY 5 cut(s) 37, 64, 158, 328, 598
BstYI RGATCY 5 cut(s) 37, 64, 158, 328, 598
BstZI CGGCCG 1 cut(s) 516
BsuRI GGCC 2 cut(s) 117, 518
BtgI CCRYGG 1 cut(s) 552
BtgZI GCGATG 3 cut(s) 76, 272, 285
BtsCI GGATG 1 cut(s) 681
Cac8I GCNNGC 1 cut(s) 21
Cfr10I RCCGGY 1 cut(s) 80
Csp6I GTAC 1 cut(s) 277
CspAI ACCGGT 1 cut(s) 80
CviAII CATG 4 cut(s) 171, 186, 274, 390
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 4 cut(s) 68, 162, 434, 594
DpnI GATC 7 cut(s) 39, 66, 160, 330, 600, 623, 661
DpnII GATC 7 cut(s) 37, 64, 158, 328, 598, 621, 659
DraI TTTAAA 1 cut(s) 750
DraIII CACNNNGTG 1 cut(s) 437
EaeI YGGCCR 1 cut(s) 516
EagI CGGCCG 1 cut(s) 516
Eam1104I CTCTTC 2 cut(s) 344, 579
EarI CTCTTC 2 cut(s) 344, 579
EclXI CGGCCG 1 cut(s) 516
Eco52I CGGCCG 1 cut(s) 516
Eco88I CYCGRG 1 cut(s) 690
EcoT22I ATGCAT 1 cut(s) 649
Esp3I CGTCTC 2 cut(s) 312, 388
FaeI CATG 4 cut(s) 174, 189, 277, 393
FaqI GGGAC 2 cut(s) 258, 570
FatI CATG 4 cut(s) 170, 185, 273, 389
FauI CCCGC 1 cut(s) 197
Fnu4HI GCNGC 3 cut(s) 73, 369, 516
FokI GGATG 1 cut(s) 688
Fsp4HI GCNGC 3 cut(s) 73, 369, 516
FspBI CTAG 4 cut(s) 248, 480, 488, 548
GluI GCNGC 3 cut(s) 73, 369, 516
HaeIII GGCC 2 cut(s) 117, 518
HapII CCGG 3 cut(s) 81, 496, 519
Hin1I GRCGYC 1 cut(s) 725
Hin1II CATG 4 cut(s) 174, 189, 277, 393
HinfI GANTC 4 cut(s) 335, 443, 588, 719
HpaII CCGG 3 cut(s) 81, 496, 519
HphI GGTGA 1 cut(s) 286
Hpy188I TCNGA 1 cut(s) 675
Hpy188III TCNNGA 6 cut(s) 6, 35, 332, 447, 602, 692
Hpy99I CGWCG 1 cut(s) 727
HpyAV CCTTC 2 cut(s) 382, 689
HpyCH4III ACNGT 4 cut(s) 423, 541, 685, 709
HpyCH4IV ACGT 1 cut(s) 725
HpyCH4V TGCA 3 cut(s) 431, 647, 761
HpyF10VI GCNNNNNNNGC 4 cut(s) 123, 186, 578, 686
HpyF3I CTNAG 4 cut(s) 68, 162, 434, 594
HpySE526I ACGT 1 cut(s) 725
Hsp92I GRCGYC 1 cut(s) 725
Hsp92II CATG 4 cut(s) 174, 189, 277, 393
Kzo9I GATC 7 cut(s) 37, 64, 158, 328, 598, 621, 659
LguI GCTCTTC 1 cut(s) 579
Lsp1109I GCAGC 2 cut(s) 84, 355
LweI GCATC 2 cut(s) 641, 656
MaeI CTAG 4 cut(s) 248, 480, 488, 548
MaeII ACGT 1 cut(s) 725
MaeIII GTNAC 2 cut(s) 25, 292
MalI GATC 7 cut(s) 39, 66, 160, 330, 600, 623, 661
MbiI CCGCTC 1 cut(s) 572
MboI GATC 7 cut(s) 37, 64, 158, 328, 598, 621, 659
MboII GAAGA 4 cut(s) 361, 367, 370, 566
MflI RGATCY 5 cut(s) 37, 64, 158, 328, 598
MlyI GAGTC 1 cut(s) 329
MmeI TCCRAC 1 cut(s) 388
MnlI CCTC 6 cut(s) 128, 149, 345, 406, 622, 663
Mph1103I ATGCAT 1 cut(s) 649
MseI TTAA 1 cut(s) 749
MspA1I CMGCKG 1 cut(s) 515
MspI CCGG 3 cut(s) 81, 496, 519
MwoI GCNNNNNNNGC 4 cut(s) 123, 186, 578, 686
NdeII GATC 7 cut(s) 37, 64, 158, 328, 598, 621, 659
NlaIII CATG 4 cut(s) 174, 189, 277, 393
NlaIV GGNNCC 1 cut(s) 330
NmuCI GTSAC 2 cut(s) 25, 292
NsiI ATGCAT 1 cut(s) 649
NspI RCATGY 2 cut(s) 189, 277
PaeR7I CTCGAG 1 cut(s) 690
PciI ACATGT 1 cut(s) 273
PciSI GCTCTTC 1 cut(s) 579
PfeI GAWTC 3 cut(s) 443, 588, 719
PinAI ACCGGT 1 cut(s) 80
PkrI GCNGC 3 cut(s) 74, 370, 517
PleI GAGTC 1 cut(s) 329
PpsI GAGTC 1 cut(s) 329
PscI ACATGT 1 cut(s) 273
PsiI TTATAA 1 cut(s) 536
PspN4I GGNNCC 1 cut(s) 330
PsuI RGATCY 5 cut(s) 37, 64, 158, 328, 598
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SapI GCTCTTC 1 cut(s) 579
SaqAI TTAA 1 cut(s) 749
SatI GCNGC 3 cut(s) 73, 369, 516
Sau3AI GATC 7 cut(s) 37, 64, 158, 328, 598, 621, 659
SchI GAGTC 1 cut(s) 329
SetI ASST 7 cut(s) 25, 370, 417, 481, 567, 700, 728
SfaNI GCATC 2 cut(s) 641, 656
Sfr274I CTCGAG 1 cut(s) 690
SlaI CTCGAG 1 cut(s) 690
SmlI CTYRAG 1 cut(s) 690
SmoI CTYRAG 1 cut(s) 690
SsiI CCGC 4 cut(s) 204, 251, 515, 570
SspMI CTAG 4 cut(s) 248, 480, 488, 548
TaaI ACNGT 4 cut(s) 423, 541, 685, 709
TaiI ACGT 1 cut(s) 728
TaqI TCGA 5 cut(s) 5, 586, 627, 691, 722
TatI WGTACW 1 cut(s) 276
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 3 cut(s) 443, 588, 719
Tru1I TTAA 1 cut(s) 749
Tru9I TTAA 1 cut(s) 749
TseFI GTSAC 2 cut(s) 25, 292
TseI GCWGC 2 cut(s) 72, 368
Tsp45I GTSAC 2 cut(s) 25, 292
TspDTI ATGAA 2 cut(s) 79, 732
XceI RCATGY 2 cut(s) 189, 277
XhoI CTCGAG 1 cut(s) 690
XspI CTAG 4 cut(s) 248, 480, 488, 548
ZraI GACGTC 1 cut(s) 726
Zsp2I ATGCAT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.