Prupe.1G125800_v2.0.a1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014135
Physical Location & Seq
Forward (+)
9911604 .. 9913144
1541 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G125800.1

Sequence Viewer

Length: 792 bp
ATGGCAAAGAGAATGGCAGTCACACACGCAGACCTTGCACCAAGTCGTCCCGACACAGGCTTTGGTAGCAAAACAGGTGCTTTTCTCACGGTCTTGACCATTCTCTGTGGCCTGTTCTGCTTCATTCTATGCCTAATTGCTGAAGTCACTCGTTCTAAGGTCACATGGCAGATCAGCAGTGATGAAGGGGATGAGGGAGGAAAATCCCAATGTGTATATAGCGGCAGTGGGAAATCGCCGCTGTTATGTGCAGCCAGTGCATTTTTGGGGCTGGCAATCATCATGGTGGTGGAACATGCATACATGTTGATTGCAATTAGCAACTCCCCTCCATCAGTCTTGGTTATTTGGGAGCCTGATCATTCTGGTCCTGCCAAGAGTTTGAAATGGCAAGCTGCCTTCTTCTTTGTTGCAACTTGGGTTTCCTTTGCTGTTGGGGAGATCTTGTTGTTGATTGGGCTAAGTGTGGAGTCAGGGCACTTGAGAAAATGGTCAAGACCAAGACCCAGCTGCCTCGTTCTCAGAGAAGGTGTGTTCTCTGCAGCTGGAGTGTTTGCCTTGACCACTGTGTTGCTTGCTGCTGGGTTATACTTGACTGCATTGCGAGCACAAAGAATCTCCCTGCACCAAGAAACCGTCCGGCGAGAAATCGTTGAGGCCTCCATACTCTATGCATCTCCACCAACCTCACCTCAGATCTCAACCATTCCTAGAGAAAACCCCATTTTCAGAGAAACACACAACATTGATCACCAGCCTCCAGCCGCTCTTAGCAAACATTTGAATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

264

Amino Acids

28.44

Weight (kDa)

8.56

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49320
fragaria_vesca FvH4_4g19280
malus_domestica MD13G1158800.v1.1 MD16G1159000.v1.1
prunus_persica Prupe.1G125800_v2.0.a1
pyrus_communis pycom13g13570 pycom16g13400
rosa_chinensis RchiOBHm_Chr4g0424271
rosa_laevigata RLG00000007491
rosa_multiflora Rmu_sc0002260.1_g000024
rosa_roxburghii Rroxscaffold_5G00366210
rosa_rugosa Rorug04G0194900
rosa_samantha Rh4AG252200 Rh4BG257700 Rh4CG268300 Rh4DG252000
rosa_wichuraiana Rw4G021750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 767
AciI CCGC 3 cut(s) 222, 239, 765
AcsI RAATTY 1 cut(s) 784
AcuI CTGAAG 1 cut(s) 162
AfiI CCNNNNNNNGG 1 cut(s) 56
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 2 cut(s) 385, 784
AluBI AGCT 3 cut(s) 395, 510, 545
AluI AGCT 3 cut(s) 395, 510, 545
Alw21I GWGCWC 1 cut(s) 610
AoxI GGCC 2 cut(s) 109, 657
ApeKI GCWGC 5 cut(s) 251, 395, 510, 542, 578
ApoI RAATTY 1 cut(s) 784
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AspS9I GGNCC 1 cut(s) 368
AsuHPI GGTGA 2 cut(s) 681, 743
AvaII GGWCC 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 480
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 5 cut(s) 263, 382, 497, 554, 565
BccI CCATC 1 cut(s) 340
BclI TGATCA 2 cut(s) 358, 748
BfaI CTAG 1 cut(s) 711
BfmI CTRYAG 1 cut(s) 540
BglII AGATCT 2 cut(s) 441, 696
BisI GCNGC 8 cut(s) 223, 239, 252, 396, 511, 543, 579, 765
BlsI GCNGC 8 cut(s) 224, 240, 253, 397, 512, 544, 580, 766
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 1 cut(s) 354
BmsI GCATC 1 cut(s) 683
BpmI CTGGAG 2 cut(s) 567, 744
BpuEI CTTGAG 1 cut(s) 502
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bsc4I CCNNNNNNNGG 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 255
Bse3DI GCAATG 1 cut(s) 599
BseGI GGATG 1 cut(s) 196
BseLI CCNNNNNNNGG 1 cut(s) 56
BseMI GCAATG 1 cut(s) 599
BseMII CTCAG 2 cut(s) 535, 707
BseNI ACTGG 1 cut(s) 255
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 5 cut(s) 263, 382, 497, 554, 565
BseYI CCCAGC 2 cut(s) 506, 581
BsgI GTGCAG 2 cut(s) 270, 608
BshFI GGCC 2 cut(s) 111, 659
BsiHKAI GWGCWC 1 cut(s) 610
BsiSI CCGG 1 cut(s) 640
BslFI GGGAC 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 56
BsmFI GGGAC 1 cut(s) 33
BsnI GGCC 2 cut(s) 111, 659
Bsp1286I GDGCHC 2 cut(s) 480, 610
Bsp143I GATC 5 cut(s) 171, 358, 441, 696, 748
BspACI CCGC 3 cut(s) 222, 239, 765
BspANI GGCC 2 cut(s) 111, 659
BspCNI CTCAG 2 cut(s) 534, 706
BspLI GGNNCC 1 cut(s) 354
BspMAI CTGCAG 1 cut(s) 544
BsrBI CCGCTC 1 cut(s) 767
BsrDI GCAATG 1 cut(s) 599
BsrI ACTGG 1 cut(s) 255
BssMI GATC 5 cut(s) 171, 358, 441, 696, 748
Bst4CI ACNGT 3 cut(s) 91, 568, 637
BstAPI GCANNNNNTGC 2 cut(s) 35, 257
BstC8I GCNNGC 4 cut(s) 273, 393, 576, 606
BstDEI CTNAG 5 cut(s) 156, 461, 521, 693, 770
BstF5I GGATG 1 cut(s) 196
BstKTI GATC 5 cut(s) 174, 361, 444, 699, 751
BstMBI GATC 5 cut(s) 171, 358, 441, 696, 748
BstMWI GCNNNNNNNGC 5 cut(s) 35, 66, 117, 257, 605
BstNSI RCATGY 2 cut(s) 299, 307
BstSFI CTRYAG 1 cut(s) 540
BstSLI GKGCMC 1 cut(s) 480
BstV1I GCAGC 5 cut(s) 263, 382, 497, 554, 565
BstX2I RGATCY 2 cut(s) 441, 696
BstYI RGATCY 2 cut(s) 441, 696
BsuRI GGCC 2 cut(s) 111, 659
BtsCI GGATG 1 cut(s) 196
BtsI GCAGTG 2 cut(s) 184, 232
BtsIMutI CAGTG 4 cut(s) 184, 232, 262, 564
Cac8I GCNNGC 4 cut(s) 273, 393, 576, 606
Cfr13I GGNCC 1 cut(s) 368
CviAII CATG 4 cut(s) 165, 283, 296, 304
DdeI CTNAG 5 cut(s) 156, 461, 521, 693, 770
DpnI GATC 5 cut(s) 173, 360, 443, 698, 750
DpnII GATC 5 cut(s) 171, 358, 441, 696, 748
Eco147I AGGCCT 1 cut(s) 659
Eco47I GGWCC 1 cut(s) 368
Eco57I CTGAAG 1 cut(s) 162
EcoT22I ATGCAT 2 cut(s) 301, 676
FaeI CATG 4 cut(s) 168, 286, 299, 307
FaqI GGGAC 1 cut(s) 33
FatI CATG 4 cut(s) 164, 282, 295, 303
FbaI TGATCA 2 cut(s) 358, 748
Fnu4HI GCNGC 8 cut(s) 223, 239, 252, 396, 511, 543, 579, 765
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 8 cut(s) 223, 239, 252, 396, 511, 543, 579, 765
FspBI CTAG 1 cut(s) 711
GluI GCNGC 8 cut(s) 223, 239, 252, 396, 511, 543, 579, 765
GsaI CCCAGC 2 cut(s) 510, 585
GsuI CTGGAG 2 cut(s) 567, 744
HaeIII GGCC 2 cut(s) 111, 659
HapII CCGG 1 cut(s) 640
Hin1II CATG 4 cut(s) 168, 286, 299, 307
HinfI GANTC 2 cut(s) 470, 615
HpaII CCGG 1 cut(s) 640
HphI GGTGA 2 cut(s) 681, 743
Hpy188I TCNGA 3 cut(s) 524, 696, 731
Hpy188III TCNNGA 3 cut(s) 50, 94, 495
HpyAV CCTTC 3 cut(s) 179, 409, 521
HpyCH4III ACNGT 3 cut(s) 91, 568, 637
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 66, 117, 257, 605
HpyF3I CTNAG 5 cut(s) 156, 461, 521, 693, 770
Hsp92II CATG 4 cut(s) 168, 286, 299, 307
Ksp22I TGATCA 2 cut(s) 358, 748
Kzo9I GATC 5 cut(s) 171, 358, 441, 696, 748
LmnI GCTCC 1 cut(s) 352
Lsp1109I GCAGC 5 cut(s) 263, 382, 497, 554, 565
LweI GCATC 1 cut(s) 683
MaeI CTAG 1 cut(s) 711
MaeIII GTNAC 3 cut(s) 19, 145, 160
MalI GATC 5 cut(s) 173, 360, 443, 698, 750
MbiI CCGCTC 1 cut(s) 767
MboI GATC 5 cut(s) 171, 358, 441, 696, 748
MboII GAAGA 1 cut(s) 394
MflI RGATCY 2 cut(s) 441, 696
MhlI GDGCHC 2 cut(s) 480, 610
MluCI AATT 3 cut(s) 135, 315, 784
MlyI GAGTC 1 cut(s) 479
MnlI CCTC 9 cut(s) 187, 191, 339, 524, 649, 670, 697, 702, 768
Mph1103I ATGCAT 2 cut(s) 301, 676
MslI CAYNNNNRTG 2 cut(s) 284, 287
MspA1I CMGCKG 3 cut(s) 241, 510, 545
MspI CCGG 1 cut(s) 640
MwoI GCNNNNNNNGC 5 cut(s) 35, 66, 117, 257, 605
NdeII GATC 5 cut(s) 171, 358, 441, 696, 748
NlaIII CATG 4 cut(s) 168, 286, 299, 307
NlaIV GGNNCC 1 cut(s) 354
NmuCI GTSAC 3 cut(s) 19, 145, 160
NsiI ATGCAT 2 cut(s) 301, 676
NspI RCATGY 2 cut(s) 299, 307
PceI AGGCCT 1 cut(s) 659
PciI ACATGT 1 cut(s) 303
PfeI GAWTC 1 cut(s) 615
PkrI GCNGC 8 cut(s) 224, 240, 253, 397, 512, 544, 580, 766
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
PscI ACATGT 1 cut(s) 303
PspFI CCCAGC 2 cut(s) 506, 581
PspN4I GGNNCC 1 cut(s) 354
PspPI GGNCC 1 cut(s) 368
PstI CTGCAG 1 cut(s) 544
PsuI RGATCY 2 cut(s) 441, 696
PvuII CAGCTG 2 cut(s) 510, 545
RseI CAYNNNNRTG 2 cut(s) 284, 287
SatI GCNGC 8 cut(s) 223, 239, 252, 396, 511, 543, 579, 765
Sau3AI GATC 5 cut(s) 171, 358, 441, 696, 748
Sau96I GGNCC 1 cut(s) 368
SchI GAGTC 1 cut(s) 479
SduI GDGCHC 2 cut(s) 480, 610
SetI ASST 9 cut(s) 36, 79, 162, 397, 512, 532, 547, 689, 694
SfaNI GCATC 1 cut(s) 683
SfcI CTRYAG 1 cut(s) 540
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 2 cut(s) 284, 287
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
Sse9I AATT 3 cut(s) 135, 315, 784
SseBI AGGCCT 1 cut(s) 659
SsiI CCGC 3 cut(s) 222, 239, 765
SspMI CTAG 1 cut(s) 711
StuI AGGCCT 1 cut(s) 659
TaaI ACNGT 3 cut(s) 91, 568, 637
TasI AATT 3 cut(s) 135, 315, 784
TauI GCSGC 3 cut(s) 225, 241, 767
TfiI GAWTC 1 cut(s) 615
TscAI CASTG 4 cut(s) 184, 232, 262, 571
TseFI GTSAC 3 cut(s) 19, 145, 160
TseI GCWGC 5 cut(s) 251, 395, 510, 542, 578
Tsp45I GTSAC 3 cut(s) 19, 145, 160
TspDTI ATGAA 2 cut(s) 112, 198
TspRI CASTG 4 cut(s) 184, 232, 262, 571
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 1 cut(s) 784
XceI RCATGY 2 cut(s) 299, 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 262
XspI CTAG 1 cut(s) 711
Zsp2I ATGCAT 2 cut(s) 301, 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.