MD16G1159000.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014135
Physical Location & Seq
Forward (+)
12768889 .. 12770457
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1159000.v1.1.491

Sequence Viewer

Length: 786 bp
ATGCCAAAGATAATGGCAGTCACACATGCAGACCTTTCACCAAGTCGTCCAACCACCGGTTTAGGTAGCAAAACAGGCGCTGTCATCATGGTCTTAACCATTCTCAGCGGCCTCTTCTGCTTCATCTTATGTCTAATTGCTGAAGCTACCCGTTCCAAGGTAACATGGTTGAGTAGTGATGAAGGGGAAAATGAATGTGAATACAGCGGCAGCGGGAAAACGCCGCTGCTATGTGCAGCCAGTGCATTTCTGGGGCTGGCTGTTATCATGGTGGTGGAACATGGATACATGTTGTTTGCAATTAGTAACTCACCTCCATCAGCCTTGGCTTTTTGGGAGCCTCATTATTCTGGTCCTGCAAAGAGCTTGAAATGGCAAGCTGCCTTTTTCTTTGTTTCAACTTGGGTTTGTTTTGCTGTTGGCGAGATCTTGTTATTGATTGGACTAAGCGTTGAGTCAGGGCACTTGAGAAAATGGTCAAGGCCAAGACCCATTTGCCTTGTCCTCAGAGAAGGTGTGTTATCTGCTGCTGGGGTGTTTGCTTTGACAACAGTTTTCCTTGCTGCTGGGTTATACTTGACTGCATCGAGAGCACAAAGAATCTCCCAACGCCAAGAAACTTTGAGGAGAGAAATGATTGAAGCCTCCATACTCTATGCCTCTCCGCCAAGGTCACCTCCTCTCACAACCATTCCTAGGGAGAACCCTTTGTTTAGAGAAAATCACATTGAGCAACCACCGTTGATTGTGAATCCGACGGCTCCTAGCAAACAATTAAATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

262

Amino Acids

28.41

Weight (kDa)

8.78

Isoelectric Point (pI)

52.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 82 - 185 1.5e-17 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49320
fragaria_vesca FvH4_4g19280
malus_domestica MD13G1158800.v1.1 MD16G1159000.v1.1
prunus_persica Prupe.1G125800_v2.0.a1
pyrus_communis pycom13g13570 pycom16g13400
rosa_chinensis RchiOBHm_Chr4g0424271
rosa_laevigata RLG00000007491
rosa_multiflora Rmu_sc0002260.1_g000024
rosa_roxburghii Rroxscaffold_5G00366210
rosa_rugosa Rorug04G0194900
rosa_samantha Rh4AG252200 Rh4BG257700 Rh4CG268300 Rh4DG252000
rosa_wichuraiana Rw4G021750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 108, 207, 213, 224, 665
AcsI RAATTY 1 cut(s) 778
AcuI CTGAAG 1 cut(s) 162
AfiI CCNNNNNNNGG 3 cut(s) 56, 157, 696
AflIII ACRYGT 1 cut(s) 288
AgeI ACCGGT 1 cut(s) 56
AgsI TTSAA 3 cut(s) 370, 399, 641
AluBI AGCT 3 cut(s) 146, 366, 380
AluI AGCT 3 cut(s) 146, 366, 380
Alw21I GWGCWC 1 cut(s) 595
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 2 cut(s) 109, 482
ApeKI GCWGC 6 cut(s) 210, 226, 236, 380, 527, 563
ApoI RAATTY 1 cut(s) 778
AsiGI ACCGGT 1 cut(s) 56
AspA2I CCTAGG 1 cut(s) 695
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 3 cut(s) 30, 303, 666
AvaII GGWCC 1 cut(s) 353
AvrII CCTAGG 1 cut(s) 695
BaeGI GKGCMC 1 cut(s) 465
BarI GAAGNNNNNNTAC 2 cut(s) 633, 665
Bbv12I GWGCWC 1 cut(s) 595
BbvI GCAGC 6 cut(s) 213, 222, 248, 367, 514, 550
BccI CCATC 1 cut(s) 325
BceAI ACGGC 1 cut(s) 774
BciVI GTATCC 1 cut(s) 278
BfaI CTAG 2 cut(s) 696, 765
BfoI RGCGCY 1 cut(s) 81
BfuI GTATCC 1 cut(s) 278
BglII AGATCT 1 cut(s) 426
BisI GCNGC 9 cut(s) 109, 208, 211, 224, 227, 237, 381, 528, 564
BlnI CCTAGG 1 cut(s) 695
BlsI GCNGC 9 cut(s) 110, 209, 212, 225, 228, 238, 382, 529, 565
Bme18I GGWCC 1 cut(s) 353
BmgT120I GGNCC 1 cut(s) 353
BmiI GGNNCC 2 cut(s) 339, 762
BmsI GCATC 1 cut(s) 593
BpuEI CTTGAG 1 cut(s) 487
BsaJI CCNNGG 4 cut(s) 156, 324, 668, 695
BsaWI WCCGGW 1 cut(s) 56
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 157, 696
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 240
BseDI CCNNGG 4 cut(s) 156, 324, 668, 695
BseLI CCNNNNNNNGG 3 cut(s) 56, 157, 696
BseMII CTCAG 2 cut(s) 118, 520
BseNI ACTGG 1 cut(s) 240
BseRI GAGGAG 2 cut(s) 640, 669
BseSI GKGCMC 1 cut(s) 465
BseXI GCAGC 6 cut(s) 213, 222, 248, 367, 514, 550
BseYI CCCAGC 2 cut(s) 530, 566
BsgI GTGCAG 1 cut(s) 255
BshFI GGCC 2 cut(s) 111, 484
BshTI ACCGGT 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 595
BsiSI CCGG 1 cut(s) 57
BslI CCNNNNNNNGG 3 cut(s) 56, 157, 696
BsnI GGCC 2 cut(s) 111, 484
Bsp1286I GDGCHC 2 cut(s) 465, 595
Bsp143I GATC 1 cut(s) 426
BspACI CCGC 5 cut(s) 108, 207, 213, 224, 665
BspANI GGCC 2 cut(s) 111, 484
BspCNI CTCAG 2 cut(s) 117, 519
BspLI GGNNCC 2 cut(s) 339, 762
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 1 cut(s) 240
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 4 cut(s) 156, 324, 668, 695
BssMI GATC 1 cut(s) 426
BssT1I CCWWGG 4 cut(s) 156, 324, 668, 695
Bst4CI ACNGT 2 cut(s) 553, 741
Bst6I CTCTTC 1 cut(s) 119
BstAPI GCANNNNNTGC 1 cut(s) 242
BstC8I GCNNGC 2 cut(s) 258, 378
BstDEI CTNAG 3 cut(s) 104, 446, 506
BstEII GGTNACC 1 cut(s) 672
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 1 cut(s) 429
BstMBI GATC 1 cut(s) 426
BstMWI GCNNNNNNNGC 4 cut(s) 75, 117, 242, 590
BstNSI RCATGY 2 cut(s) 29, 292
BstPI GGTNACC 1 cut(s) 672
BstSLI GKGCMC 1 cut(s) 465
BstV1I GCAGC 6 cut(s) 213, 222, 248, 367, 514, 550
BstX2I RGATCY 1 cut(s) 426
BstYI RGATCY 1 cut(s) 426
BsuI GTATCC 1 cut(s) 278
BsuRI GGCC 2 cut(s) 111, 484
BtsIMutI CAGTG 1 cut(s) 247
Cac8I GCNNGC 2 cut(s) 258, 378
CaiI CAGNNNCTG 1 cut(s) 80
CfoI GCGC 1 cut(s) 80
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 353
CspAI ACCGGT 1 cut(s) 56
CviAII CATG 6 cut(s) 26, 88, 165, 268, 281, 289
DdeI CTNAG 3 cut(s) 104, 446, 506
DpnI GATC 1 cut(s) 428
DpnII GATC 1 cut(s) 426
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
EciI GGCGGA 1 cut(s) 654
Eco130I CCWWGG 4 cut(s) 156, 324, 668, 695
Eco47I GGWCC 1 cut(s) 353
Eco57I CTGAAG 1 cut(s) 162
Eco91I GGTNACC 1 cut(s) 672
EcoO65I GGTNACC 1 cut(s) 672
EcoT14I CCWWGG 4 cut(s) 156, 324, 668, 695
ErhI CCWWGG 4 cut(s) 156, 324, 668, 695
FaeI CATG 6 cut(s) 29, 91, 168, 271, 284, 292
FatI CATG 6 cut(s) 25, 87, 164, 267, 280, 288
FauI CCCGC 1 cut(s) 206
Fnu4HI GCNGC 9 cut(s) 109, 208, 211, 224, 227, 237, 381, 528, 564
Fsp4HI GCNGC 9 cut(s) 109, 208, 211, 224, 227, 237, 381, 528, 564
FspBI CTAG 2 cut(s) 696, 765
GlaI GCGC 1 cut(s) 79
GluI GCNGC 9 cut(s) 109, 208, 211, 224, 227, 237, 381, 528, 564
GsaI CCCAGC 2 cut(s) 534, 570
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 2 cut(s) 111, 484
HapII CCGG 1 cut(s) 57
HhaI GCGC 1 cut(s) 80
Hin1II CATG 6 cut(s) 29, 91, 168, 271, 284, 292
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HinfI GANTC 3 cut(s) 455, 600, 751
HpaII CCGG 1 cut(s) 57
HphI GGTGA 3 cut(s) 30, 303, 666
Hpy188I TCNGA 2 cut(s) 509, 756
Hpy188III TCNNGA 1 cut(s) 588
Hpy99I CGWCG 1 cut(s) 760
HpyAV CCTTC 2 cut(s) 176, 506
HpyCH4III ACNGT 2 cut(s) 553, 741
HpyCH4V TGCA 6 cut(s) 29, 236, 245, 299, 359, 584
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 117, 242, 590
HpyF3I CTNAG 3 cut(s) 104, 446, 506
Hsp92II CATG 6 cut(s) 29, 91, 168, 271, 284, 292
HspAI GCGC 1 cut(s) 78
Kzo9I GATC 1 cut(s) 426
LmnI GCTCC 2 cut(s) 337, 766
Lsp1109I GCAGC 6 cut(s) 213, 222, 248, 367, 514, 550
LweI GCATC 1 cut(s) 593
MaeI CTAG 2 cut(s) 696, 765
MaeIII GTNAC 4 cut(s) 19, 160, 305, 672
MalI GATC 1 cut(s) 428
MboI GATC 1 cut(s) 426
MboII GAAGA 1 cut(s) 106
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 2 cut(s) 465, 595
MluCI AATT 4 cut(s) 135, 300, 773, 778
MlyI GAGTC 1 cut(s) 464
MmeI TCCRAC 2 cut(s) 74, 779
MnlI CCTC 9 cut(s) 122, 324, 351, 515, 618, 655, 670, 687, 690
MseI TTAA 2 cut(s) 95, 776
MslI CAYNNNNRTG 1 cut(s) 272
MspA1I CMGCKG 4 cut(s) 108, 207, 213, 226
MspI CCGG 1 cut(s) 57
MwoI GCNNNNNNNGC 4 cut(s) 75, 117, 242, 590
NdeII GATC 1 cut(s) 426
NlaIII CATG 6 cut(s) 29, 91, 168, 271, 284, 292
NlaIV GGNNCC 2 cut(s) 339, 762
NmuCI GTSAC 2 cut(s) 19, 672
NspI RCATGY 2 cut(s) 29, 292
PciI ACATGT 1 cut(s) 288
PfeI GAWTC 2 cut(s) 600, 751
PinAI ACCGGT 1 cut(s) 56
PkrI GCNGC 9 cut(s) 110, 209, 212, 225, 228, 238, 382, 529, 565
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
PscI ACATGT 1 cut(s) 288
PspEI GGTNACC 1 cut(s) 672
PspFI CCCAGC 2 cut(s) 530, 566
PspN4I GGNNCC 2 cut(s) 339, 762
PspPI GGNCC 1 cut(s) 353
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 1 cut(s) 426
RseI CAYNNNNRTG 1 cut(s) 272
SaqAI TTAA 2 cut(s) 95, 776
SatI GCNGC 9 cut(s) 109, 208, 211, 224, 227, 237, 381, 528, 564
Sau3AI GATC 1 cut(s) 426
Sau96I GGNCC 1 cut(s) 353
SchI GAGTC 1 cut(s) 464
SduI GDGCHC 2 cut(s) 465, 595
SfaNI GCATC 1 cut(s) 593
SinI GGWCC 1 cut(s) 353
SmiMI CAYNNNNRTG 1 cut(s) 272
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
Sse9I AATT 4 cut(s) 135, 300, 773, 778
SsiI CCGC 5 cut(s) 108, 207, 213, 224, 665
SspMI CTAG 2 cut(s) 696, 765
StyI CCWWGG 4 cut(s) 156, 324, 668, 695
TaaI ACNGT 2 cut(s) 553, 741
TaqI TCGA 1 cut(s) 587
TasI AATT 4 cut(s) 135, 300, 773, 778
TauI GCSGC 3 cut(s) 111, 210, 226
TfiI GAWTC 2 cut(s) 600, 751
Tru1I TTAA 2 cut(s) 95, 776
Tru9I TTAA 2 cut(s) 95, 776
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 2 cut(s) 19, 672
TseI GCWGC 6 cut(s) 210, 226, 236, 380, 527, 563
Tsp45I GTSAC 2 cut(s) 19, 672
TspDTI ATGAA 3 cut(s) 112, 195, 207
TspRI CASTG 1 cut(s) 247
VpaK11BI GGWCC 1 cut(s) 353
XapI RAATTY 1 cut(s) 778
XceI RCATGY 2 cut(s) 29, 292
XcmI CCANNNNNNNNNTGG 1 cut(s) 247
XmaJI CCTAGG 1 cut(s) 695
XspI CTAG 2 cut(s) 696, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.