Rmu_sc0002260.1_g000024

Belongs to the importin alpha family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002260.1
OG0014135
Physical Location & Seq
Forward (+)
90644 .. 96685
6042 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002260.1_g000024.1.cds

Sequence Viewer

Length: 1926 bp
atgccaaagacaaaggcagtcacacatgcagatctttcaccgagccgcccacactccgccctgggcagcaaaacaggccttcttctcattgtcttgaccattctttgtggcctcttttgcttcattctatgcctaattgctgaagccactcgttctcgggtttcatggctgagcaacagtactgatgaagatgggaagggaggaaaatacgagtgtatatatagtggtagtgggaaaacacctttgttaagtgcagccagtgcatttttgggattggcaattatcatggtggtggagcatggatacatgttgtatgcaattaccaactccccaccttcggtcttgcttacatgggagccgcatgattcagcttctgccaagtctttgaaatggcatgccgccattttcttcgtcgcaacttggatctgttttgctgttggggagattttgctattgattggccttagtgtggagtcaggccatttgaaaaactgggctaggccaaaacccagttgccttgtgatcagagaaggtgtgttctctgcggcaggagtgttttccttgactactgtttttcttgctgccggattatacctgacggcgttgcgtgcagagagaatttcccaagaccaagaaaatgtaaggcgagaggtgctcgaggccgctgtactttatgcatctccaccaagaaaagaagaggcggagaacgtgccggtgaaccctaaggacttaacggcgacgggccaaaccagctcggagttttcaagagctccgtttaacctgattcagaaccctaagccggaacagaagtcggcgatttggagtagggctgagaagacgccgactaaagcttccaaggccaaagcttccgatccggggatgcctcttcggacaccggaaaagcacggtgctgcgttttcggctcggaaacggttcggttgggcccagaggaacgattcaagtgattttcatgaggatgggggtgcttgtacgactcaagtgagcagaggacgtgttagggtttcagaagcaaaaaacgacatgtcgctcaggccgaacgccaggactgaggtccgccggaaccggtacaaggtggcggtggacgccgaggagggccgtcgccggagagaggacaacatggtcgagatccggaagagcaagagggaggagagtttgttaaagaagcgccgcgaaggtcttcaagctcagcaattcccggcctctgttcagccaccgaacctcgagaagaagttggagagtctaccgtcgatggttgccggggtttggtccaacgaaagtcctctgcagctggaggctactacgcagtttcggaaactactttctatagaaagaagccctccaattgaggaagtaattcaagctggggttgttccgcggttcgttgagttccttgtcagggaggattatccacaacttcagtttgaggctgcttgggctcttacaaacatagcatcaggaacatcggacaacacgagggtggtgattgatcacggggcagtcccaatattcgtgaagcttcttgcttctccgagtgatgatgttagagagcaggctgtttgggcattgggaaatgttgccggtgattctccaagatgtcgtgatctagtcctcagcagtggtgccttgatgccgttgctggctcagttgaatgagcatgcaaagctttcaatgctgaggaatgccacctggacactgtcaaacttttgcaggggcaagccgcagcctcagtttgatcaggtgaagccagctcttccagctcttgagcgtttagttcattccaatgatgaagaagtcttgacagatgcctgctgggcactctcatatctctcggatggtacaaatgacaaaattcaagcagtcatcgaggcaggtgtctgcctgcgacttgtgcagcttctcttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm Arm_3 IBB

Protein Analysis

641

Amino Acids

70.54

Weight (kDa)

8.82

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49320
fragaria_vesca FvH4_4g19280
malus_domestica MD13G1158800.v1.1 MD16G1159000.v1.1
prunus_persica Prupe.1G125800_v2.0.a1
pyrus_communis pycom13g13570 pycom16g13400
rosa_chinensis RchiOBHm_Chr4g0424271
rosa_laevigata RLG00000007491
rosa_multiflora Rmu_sc0002260.1_g000024
rosa_roxburghii Rroxscaffold_5G00366210
rosa_rugosa Rorug04G0194900
rosa_samantha Rh4AG252200 Rh4BG257700 Rh4CG268300 Rh4DG252000
rosa_wichuraiana Rw4G021750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1880
AasI GACNNNNNNGTC 1 cut(s) 1139
Acc36I ACCTGC 1 cut(s) 1880
AccB1I GGYRCC 1 cut(s) 1640
AccI GTMKAC 1 cut(s) 1261
AccII CGCG 2 cut(s) 1191, 1396
AccIII TCCGGA 1 cut(s) 1149
AclWI GGATC 3 cut(s) 431, 866, 1141
AcsI RAATTY 2 cut(s) 618, 1869
AcuI CTGAAG 2 cut(s) 162, 1421
AcyI GRCGYC 2 cut(s) 839, 1104
AfaI GTAC 5 cut(s) 181, 669, 991, 1088, 1858
AfiI CCNNNNNNNGG 8 cut(s) 337, 469, 799, 876, 1090, 1129, 1284, 1417
AflIII ACRYGT 3 cut(s) 306, 1012, 1041
AgeI ACCGGT 1 cut(s) 1083
AgsI TTSAA 9 cut(s) 388, 487, 765, 960, 1202, 1379, 1669, 1689, 1874
AjiI CACGTC 1 cut(s) 1013
AjnI CCWGG 3 cut(s) 60, 1061, 1706
AleI CACNNNNGTG 1 cut(s) 1496
Alw21I GWGCWC 2 cut(s) 657, 772
AlwI GGATC 3 cut(s) 431, 866, 1141
AlwNI CAGNNNCTG 1 cut(s) 374
Ama87I CYCGRG 3 cut(s) 156, 656, 1241
Aor13HI TCCGGA 1 cut(s) 1149
ApaI GGGCCC 1 cut(s) 946
ApeKI GCWGC 8 cut(s) 66, 254, 581, 911, 1306, 1448, 1741, 1912
ApoI RAATTY 2 cut(s) 618, 1869
AsiGI ACCGGT 1 cut(s) 1083
Asp700I GAANNNNTTC 3 cut(s) 932, 1197, 1374
AspLEI GCGC 1 cut(s) 1188
AspS9I GGNCC 6 cut(s) 742, 942, 943, 1072, 1114, 1287
AsuC2I CCSGG 3 cut(s) 876, 1217, 1279
AsuHPI GGTGA 5 cut(s) 30, 727, 1513, 1613, 1771
AvaI CYCGRG 3 cut(s) 156, 656, 1241
AvaII GGWCC 2 cut(s) 1072, 1287
AxyI CCTNAGG 1 cut(s) 723
BaeGI GKGCMC 2 cut(s) 946, 1837
BanI GGYRCC 1 cut(s) 1640
BanII GRGCYC 3 cut(s) 772, 946, 1459
BauI CACGAG 1 cut(s) 1492
BbsI GAAGAC 2 cut(s) 842, 1190
Bbv12I GWGCWC 2 cut(s) 657, 772
BbvCI CCTCAGC 2 cut(s) 1631, 1694
BbvI GCAGC 7 cut(s) 78, 266, 568, 898, 1318, 1435, 1753
BccI CCATC 4 cut(s) 185, 971, 1264, 1847
BceAI ACGGC 4 cut(s) 615, 750, 1101, 1636
BciT130I CCWGG 3 cut(s) 62, 1063, 1708
BciVI GTATCC 1 cut(s) 296
BclI TGATCA 3 cut(s) 522, 1507, 1753
BcnI CCSGG 3 cut(s) 876, 1217, 1279
BfaI CTAG 2 cut(s) 498, 1625
BfmI CTRYAG 2 cut(s) 1304, 1344
BfoI RGCGCY 1 cut(s) 1189
BfuAI ACCTGC 1 cut(s) 1880
BfuI GTATCC 1 cut(s) 296
BglI GCCNNNNNGGC 1 cut(s) 1832
BglII AGATCT 1 cut(s) 31
BlpI GCTNAGC 2 cut(s) 170, 1206
BmcAI AGTACT 1 cut(s) 181
Bme1390I CCNGG 6 cut(s) 62, 876, 1063, 1217, 1279, 1708
Bme18I GGWCC 2 cut(s) 1072, 1287
BmeT110I CYCGRG 3 cut(s) 156, 656, 1241
BmgBI CACGTC 1 cut(s) 1013
BmgT120I GGNCC 6 cut(s) 742, 942, 943, 1072, 1114, 1287
BmiI GGNNCC 4 cut(s) 357, 944, 1082, 1642
BmrFI CCNGG 6 cut(s) 62, 876, 1063, 1217, 1279, 1708
BmrI ACTGGG 2 cut(s) 502, 504
BmsI GCATC 5 cut(s) 686, 870, 1481, 1638, 1813
BmuI ACTGGG 2 cut(s) 502, 504
BoxI GACNNNNGTC 1 cut(s) 1070
BpiI GAAGAC 2 cut(s) 842, 1190
BplI GAGNNNNNCTC 2 cut(s) 639, 671
BpmI CTGGAG 1 cut(s) 1331
Bpu10I CCTNAGC 4 cut(s) 795, 1049, 1631, 1694
Bpu1102I GCTNAGC 2 cut(s) 170, 1206
BpuEI CTTGAG 2 cut(s) 981, 1802
BpuMI CCSGG 3 cut(s) 876, 1217, 1279
BsaHI GRCGYC 2 cut(s) 839, 1104
BsaJI CCNNGG 7 cut(s) 60, 61, 855, 875, 1107, 1278, 1394
BsaWI WCCGGW 3 cut(s) 895, 1083, 1149
BsaXI ACNNNNNCTCC 2 cut(s) 1160, 1190
Bsc4I CCNNNNNNNGG 8 cut(s) 337, 469, 799, 876, 1090, 1129, 1284, 1417
Bse118I RCCGGY 3 cut(s) 712, 1083, 1598
Bse1I ACTGG 3 cut(s) 258, 497, 510
Bse21I CCTNAGG 1 cut(s) 723
BseAI TCCGGA 1 cut(s) 1149
BseBI CCWGG 3 cut(s) 62, 1063, 1708
BseDI CCNNGG 7 cut(s) 60, 61, 855, 875, 1107, 1278, 1394
BseGI GGATG 3 cut(s) 885, 982, 1858
BseLI CCNNNNNNNGG 8 cut(s) 337, 469, 799, 876, 1090, 1129, 1284, 1417
BseMII CTCAG 9 cut(s) 161, 822, 1059, 1063, 1220, 1645, 1676, 1685, 1760
BseNI ACTGG 3 cut(s) 258, 497, 510
BseRI GAGGAG 2 cut(s) 1124, 1181
BseSI GKGCMC 2 cut(s) 946, 1837
BseXI GCAGC 7 cut(s) 78, 266, 568, 898, 1318, 1435, 1753
BseYI CCCAGC 2 cut(s) 1382, 1830
BsgI GTGCAG 2 cut(s) 273, 630
Bsh1236I CGCG 2 cut(s) 1191, 1396
BshNI GGYRCC 1 cut(s) 1640
BshTI ACCGGT 1 cut(s) 1083
BsiHKAI GWGCWC 2 cut(s) 657, 772
BsiHKCI CYCGRG 3 cut(s) 156, 656, 1241
BslFI GGGAC 1 cut(s) 1505
BslI CCNNNNNNNGG 8 cut(s) 337, 469, 799, 876, 1090, 1129, 1284, 1417
BsmFI GGGAC 1 cut(s) 1505
BsmI GAATGC 1 cut(s) 1705
BsoBI CYCGRG 3 cut(s) 156, 656, 1241
Bsp120I GGGCCC 1 cut(s) 942
Bsp1286I GDGCHC 5 cut(s) 657, 772, 946, 1459, 1837
Bsp13I TCCGGA 1 cut(s) 1149
Bsp143I GATC 8 cut(s) 31, 423, 522, 871, 1146, 1507, 1621, 1753
Bsp1720I GCTNAGC 2 cut(s) 170, 1206
BspCNI CTCAG 9 cut(s) 162, 823, 1060, 1062, 1219, 1644, 1675, 1686, 1759
BspEI TCCGGA 1 cut(s) 1149
BspFNI CGCG 2 cut(s) 1191, 1396
BspHI TCATGA 1 cut(s) 970
BspLI GGNNCC 4 cut(s) 357, 944, 1082, 1642
BspMAI CTGCAG 1 cut(s) 1308
BspMI ACCTGC 1 cut(s) 1880
BspPI GGATC 3 cut(s) 431, 866, 1141
BspQI GCTCTTC 2 cut(s) 1148, 1776
BspT107I GGYRCC 1 cut(s) 1640
BsrFI RCCGGY 3 cut(s) 712, 1083, 1598
BsrI ACTGG 3 cut(s) 258, 497, 510
BssAI RCCGGY 3 cut(s) 712, 1083, 1598
BssECI CCNNGG 7 cut(s) 60, 61, 855, 875, 1107, 1278, 1394
BssMI GATC 8 cut(s) 31, 423, 522, 871, 1146, 1507, 1621, 1753
BssNI GRCGYC 2 cut(s) 839, 1104
BssSI CACGAG 1 cut(s) 1492
BssT1I CCWWGG 1 cut(s) 855
Bst2BI CACGAG 1 cut(s) 1492
Bst2UI CCWGG 3 cut(s) 62, 1063, 1708
Bst4CI ACNGT 6 cut(s) 179, 571, 908, 933, 1266, 1716
Bst6I CTCTTC 4 cut(s) 690, 891, 1148, 1776
BstACI GRCGYC 2 cut(s) 839, 1104
BstAPI GCANNNNNTGC 1 cut(s) 260
BstC8I GCNNGC 9 cut(s) 396, 609, 1572, 1659, 1677, 1736, 1767, 1828, 1901
BstDSI CCRYGG 1 cut(s) 1394
BstF5I GGATG 3 cut(s) 885, 982, 1858
BstFNI CGCG 2 cut(s) 1191, 1396
BstH2I RGCGCY 1 cut(s) 1189
BstHHI GCGC 1 cut(s) 1188
BstKTI GATC 8 cut(s) 34, 426, 525, 874, 1149, 1510, 1624, 1756
BstMBI GATC 8 cut(s) 31, 423, 522, 871, 1146, 1507, 1621, 1753
BstNI CCWGG 3 cut(s) 62, 1063, 1708
BstNSI RCATGY 5 cut(s) 29, 310, 398, 1045, 1679
BstPAI GACNNNNGTC 1 cut(s) 1070
BstSCI CCNGG 6 cut(s) 60, 874, 1061, 1215, 1277, 1706
BstSFI CTRYAG 2 cut(s) 1304, 1344
BstSLI GKGCMC 2 cut(s) 946, 1837
BstUI CGCG 2 cut(s) 1191, 1396
BstV1I GCAGC 7 cut(s) 78, 266, 568, 898, 1318, 1435, 1753
BstV2I GAAGAC 2 cut(s) 842, 1190
BstX2I RGATCY 3 cut(s) 31, 423, 1146
BstYI RGATCY 3 cut(s) 31, 423, 1146
Bsu36I CCTNAGG 1 cut(s) 723
BsuI GTATCC 1 cut(s) 296
BtgI CCRYGG 1 cut(s) 1394
BtrI CACGTC 1 cut(s) 1013
BtsCI GGATG 3 cut(s) 885, 982, 1858
BtsI GCAGTG 1 cut(s) 1642
BtsIMutI CAGTG 3 cut(s) 265, 1642, 1712
BveI ACCTGC 1 cut(s) 1880
Cac8I GCNNGC 9 cut(s) 396, 609, 1572, 1659, 1677, 1736, 1767, 1828, 1901
CaiI CAGNNNCTG 1 cut(s) 374
CciI TCATGA 1 cut(s) 970
CfoI GCGC 1 cut(s) 1188
Cfr10I RCCGGY 3 cut(s) 712, 1083, 1598
Cfr13I GGNCC 6 cut(s) 742, 942, 943, 1072, 1114, 1287
Cfr42I CCGCGG 1 cut(s) 1397
CseI GACGC 2 cut(s) 847, 1112
Csp6I GTAC 5 cut(s) 180, 668, 990, 1087, 1857
CspAI ACCGGT 1 cut(s) 1083
CviQI GTAC 5 cut(s) 180, 668, 990, 1087, 1857
DpnI GATC 8 cut(s) 33, 425, 524, 873, 1148, 1509, 1623, 1755
DpnII GATC 8 cut(s) 31, 423, 522, 871, 1146, 1507, 1621, 1753
DrdI GACNNNNNNGTC 1 cut(s) 1139
DseDI GACNNNNNNGTC 1 cut(s) 1139
Eam1104I CTCTTC 4 cut(s) 690, 891, 1148, 1776
EarI CTCTTC 4 cut(s) 690, 891, 1148, 1776
EciI GGCGGA 3 cut(s) 46, 716, 1064
Ecl136II GAGCTC 1 cut(s) 770
Eco130I CCWWGG 1 cut(s) 855
Eco147I AGGCCT 1 cut(s) 78
Eco24I GRGCYC 3 cut(s) 772, 946, 1459
Eco47I GGWCC 2 cut(s) 1072, 1287
Eco53kI GAGCTC 1 cut(s) 770
Eco57I CTGAAG 2 cut(s) 162, 1421
Eco81I CCTNAGG 1 cut(s) 723
Eco88I CYCGRG 3 cut(s) 156, 656, 1241
EcoICRI GAGCTC 1 cut(s) 770
EcoRII CCWGG 3 cut(s) 60, 1061, 1706
EcoT14I CCWWGG 1 cut(s) 855
EcoT22I ATGCAT 1 cut(s) 679
EcoT38I GRGCYC 3 cut(s) 772, 946, 1459
ErhI CCWWGG 1 cut(s) 855
FaqI GGGAC 1 cut(s) 1505
FbaI TGATCA 3 cut(s) 522, 1507, 1753
FblI GTMKAC 1 cut(s) 1261
FokI GGATG 3 cut(s) 892, 989, 1865
FriOI GRGCYC 3 cut(s) 772, 946, 1459
FspBI CTAG 2 cut(s) 498, 1625
GlaI GCGC 1 cut(s) 1187
GsaI CCCAGC 2 cut(s) 1386, 1834
GsuI CTGGAG 1 cut(s) 1331
HaeII RGCGCY 1 cut(s) 1189
HgaI GACGC 2 cut(s) 847, 1112
HhaI GCGC 1 cut(s) 1188
Hin1I GRCGYC 2 cut(s) 839, 1104
Hin6I GCGC 1 cut(s) 1186
HinP1I GCGC 1 cut(s) 1186
HindIII AAGCTT 4 cut(s) 849, 864, 1535, 1682
HinfI GANTC 7 cut(s) 365, 473, 784, 956, 994, 1258, 1604
HphI GGTGA 5 cut(s) 30, 727, 1513, 1613, 1771
Hpy166II GTNNAC 3 cut(s) 718, 1102, 1262
Hpy8I GTNNAC 3 cut(s) 718, 1102, 1262
Hpy99I CGWCG 4 cut(s) 416, 742, 1122, 1270
HpyAV CCTTC 5 cut(s) 89, 190, 345, 524, 1187
HpyCH4III ACNGT 6 cut(s) 179, 571, 908, 933, 1266, 1716
HpyCH4IV ACGT 2 cut(s) 708, 1012
HpySE526I ACGT 2 cut(s) 708, 1012
Hsp92I GRCGYC 2 cut(s) 839, 1104
HspAI GCGC 1 cut(s) 1186
Kpn2I TCCGGA 1 cut(s) 1149
Ksp22I TGATCA 3 cut(s) 522, 1507, 1753
KspI CCGCGG 1 cut(s) 1397
Kzo9I GATC 8 cut(s) 31, 423, 522, 871, 1146, 1507, 1621, 1753
LguI GCTCTTC 2 cut(s) 1148, 1776
LmnI GCTCC 3 cut(s) 295, 355, 775
Lsp1109I GCAGC 7 cut(s) 78, 266, 568, 898, 1318, 1435, 1753
LweI GCATC 5 cut(s) 686, 870, 1481, 1638, 1813
MaeI CTAG 2 cut(s) 498, 1625
MaeII ACGT 2 cut(s) 708, 1012
MaeIII GTNAC 1 cut(s) 19
MalI GATC 8 cut(s) 33, 425, 524, 873, 1148, 1509, 1623, 1755
MboI GATC 8 cut(s) 31, 423, 522, 871, 1146, 1507, 1621, 1753
MfeI CAATTG 1 cut(s) 1362
MflI RGATCY 3 cut(s) 31, 423, 1146
MhlI GDGCHC 5 cut(s) 657, 772, 946, 1459, 1837
MluCI AATT 8 cut(s) 135, 279, 318, 618, 1211, 1362, 1374, 1869
MlyI GAGTC 3 cut(s) 482, 988, 1267
MmeI TCCRAC 2 cut(s) 1233, 1314
Mph1103I ATGCAT 1 cut(s) 679
MroI TCCGGA 1 cut(s) 1149
MroXI GAANNNNTTC 3 cut(s) 932, 1197, 1374
MseI TTAA 4 cut(s) 248, 731, 777, 1178
MslI CAYNNNNRTG 4 cut(s) 290, 975, 1496, 1800
MspA1I CMGCKG 3 cut(s) 665, 1309, 1396
MspR9I CCNGG 6 cut(s) 62, 876, 1063, 1217, 1279, 1708
MunI CAATTG 1 cut(s) 1362
Mva1269I GAATGC 1 cut(s) 1705
MvaI CCWGG 3 cut(s) 62, 1063, 1708
MvnI CGCG 2 cut(s) 1191, 1396
NciI CCSGG 3 cut(s) 876, 1217, 1279
NdeII GATC 8 cut(s) 31, 423, 522, 871, 1146, 1507, 1621, 1753
NlaIV GGNNCC 4 cut(s) 357, 944, 1082, 1642
NmeAIII GCCGAG 1 cut(s) 1132
NmuCI GTSAC 1 cut(s) 19
NsiI ATGCAT 1 cut(s) 679
NspI RCATGY 5 cut(s) 29, 310, 398, 1045, 1679
OliI CACNNNNGTG 1 cut(s) 1496
PaeI GCATGC 2 cut(s) 398, 1679
PaeR7I CTCGAG 2 cut(s) 656, 1241
PagI TCATGA 1 cut(s) 970
PaqCI CACCTGC 1 cut(s) 1880
PasI CCCWGGG 1 cut(s) 61
PceI AGGCCT 1 cut(s) 78
PciI ACATGT 2 cut(s) 306, 1041
PciSI GCTCTTC 2 cut(s) 1148, 1776
PcsI WCGNNNNNNNCGW 2 cut(s) 1052, 1490
PctI GAATGC 1 cut(s) 1705
PdmI GAANNNNTTC 3 cut(s) 932, 1197, 1374
PfeI GAWTC 4 cut(s) 365, 784, 956, 1604
PflFI GACNNNGTC 1 cut(s) 1714
PinAI ACCGGT 1 cut(s) 1083
PleI GAGTC 3 cut(s) 481, 988, 1266
PpsI GAGTC 3 cut(s) 481, 988, 1266
PscI ACATGT 2 cut(s) 306, 1041
PshAI GACNNNNGTC 1 cut(s) 1070
Psp124BI GAGCTC 1 cut(s) 772
Psp6I CCWGG 3 cut(s) 60, 1061, 1706
PspFI CCCAGC 2 cut(s) 1382, 1830
PspGI CCWGG 3 cut(s) 60, 1061, 1706
PspN4I GGNNCC 4 cut(s) 357, 944, 1082, 1642
PspOMI GGGCCC 1 cut(s) 942
PspPI GGNCC 6 cut(s) 742, 942, 943, 1072, 1114, 1287
PspXI VCTCGAGB 1 cut(s) 656
PstI CTGCAG 1 cut(s) 1308
PstNI CAGNNNCTG 1 cut(s) 374
PsuI RGATCY 3 cut(s) 31, 423, 1146
PsyI GACNNNGTC 1 cut(s) 1714
PvuII CAGCTG 1 cut(s) 1309
RsaI GTAC 5 cut(s) 181, 669, 991, 1088, 1858
RsaNI GTAC 5 cut(s) 180, 668, 990, 1087, 1857
RseI CAYNNNNRTG 4 cut(s) 290, 975, 1496, 1800
SacI GAGCTC 1 cut(s) 772
SacII CCGCGG 1 cut(s) 1397
SapI GCTCTTC 2 cut(s) 1148, 1776
SaqAI TTAA 4 cut(s) 248, 731, 777, 1178
Sau3AI GATC 8 cut(s) 31, 423, 522, 871, 1146, 1507, 1621, 1753
Sau96I GGNCC 6 cut(s) 742, 942, 943, 1072, 1114, 1287
ScaI AGTACT 1 cut(s) 181
SchI GAGTC 3 cut(s) 482, 988, 1267
ScrFI CCNGG 6 cut(s) 62, 876, 1063, 1217, 1279, 1708
SduI GDGCHC 5 cut(s) 657, 772, 946, 1459, 1837
SfaNI GCATC 5 cut(s) 686, 870, 1481, 1638, 1813
SfcI CTRYAG 2 cut(s) 1304, 1344
Sfr274I CTCGAG 2 cut(s) 656, 1241
Sfr303I CCGCGG 1 cut(s) 1397
SgrBI CCGCGG 1 cut(s) 1397
SinI GGWCC 2 cut(s) 1072, 1287
SlaI CTCGAG 2 cut(s) 656, 1241
SmiMI CAYNNNNRTG 4 cut(s) 290, 975, 1496, 1800
SmlI CTYRAG 4 cut(s) 656, 996, 1241, 1781
SmoI CTYRAG 4 cut(s) 656, 996, 1241, 1781
SphI GCATGC 2 cut(s) 398, 1679
Sse9I AATT 8 cut(s) 135, 279, 318, 618, 1211, 1362, 1374, 1869
SseBI AGGCCT 1 cut(s) 78
SspI AATATT 1 cut(s) 1527
SspMI CTAG 2 cut(s) 498, 1625
SstI GAGCTC 1 cut(s) 772
StuI AGGCCT 1 cut(s) 78
StyD4I CCNGG 6 cut(s) 60, 874, 1061, 1215, 1277, 1706
StyI CCWWGG 1 cut(s) 855
TaaI ACNGT 6 cut(s) 179, 571, 908, 933, 1266, 1716
TaiI ACGT 2 cut(s) 711, 1015
TaqI TCGA 5 cut(s) 657, 1143, 1242, 1268, 1884
TaqII GACCGA 1 cut(s) 328
TasI AATT 8 cut(s) 135, 279, 318, 618, 1211, 1362, 1374, 1869
TatI WGTACW 2 cut(s) 179, 667
TauI GCSGC 7 cut(s) 48, 361, 401, 548, 665, 1191, 1741
TfiI GAWTC 4 cut(s) 365, 784, 956, 1604
Tru1I TTAA 4 cut(s) 248, 731, 777, 1178
Tru9I TTAA 4 cut(s) 248, 731, 777, 1178
TscAI CASTG 3 cut(s) 265, 1642, 1719
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 8 cut(s) 66, 254, 581, 911, 1306, 1448, 1741, 1912
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 6 cut(s) 112, 153, 201, 959, 1784, 1821
TspGWI ACGGA 1 cut(s) 762
TspRI CASTG 3 cut(s) 265, 1642, 1719
Tth111I GACNNNGTC 1 cut(s) 1714
VpaK11BI GGWCC 2 cut(s) 1072, 1287
XapI RAATTY 2 cut(s) 618, 1869
XceI RCATGY 5 cut(s) 29, 310, 398, 1045, 1679
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XhoI CTCGAG 2 cut(s) 656, 1241
XmiI GTMKAC 1 cut(s) 1261
XmnI GAANNNNTTC 3 cut(s) 932, 1197, 1374
XspI CTAG 2 cut(s) 498, 1625
ZrmI AGTACT 1 cut(s) 181
Zsp2I ATGCAT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.