Rh4DG252000

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014135
Physical Location & Seq
Reverse (-)
46676776 .. 46677843
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG252000.1

Sequence Viewer

Length: 750 bp
ATGCCAAAGACAAAGGCAGTCACACATGCAGATCTTTCACCGAGCCGCCCACACTCCGCTCTGGGCAGCAAAACAGGCCTTCTTCTCATTGTCTTGACCATTATTTGTGGCCTCTTTTGCTTCATTCTATGCCTAATTGCAGAAGCCACTCGTTCTCGGGTTTCATGGCTGAGCAGCAGTACTGATGAAGATGGGAAGGGAGGAAAATACGAGTGTATATATAGTGGTAGTGGGAAAACACCTTTGTTAAGTGCAGCCAGTGCATTTTTGGGATTGGCAATTATCATGGTGGTGGAGCATGGATACATGTTGTATGCAATTACCAACTCCCCACCTTCAGTCTTGCTTACATGGGAGCCACATGATTCAGCTTCTGCCAAGTCTTTGAAATGGCATGCCGCCATTTTCTTCGTCGCAACTTGTGTGGAGTCAGGCCATTTGAAAAACTGGGCTAGGCCAAAACCCAGTTGCCTTGTGATCAGAGAAGGTGTGTTCTCTGCGGCAGGAGTGTTTTCCTTGACTACTGTTTTTCTTGCTGCCGGATTATACCTGACGGCGTTGCGTGCAGAGAGAATTTCCCAAGACCAAGAAAATGTAAGGCGAGAGGTGCTCGAGGCCGCTGTACTTTATGCATCTCCACCAAGGTCACCTCATCTCACAACCATTCCAAGGGAGAACCCCATTTTCAGAGAAAGCCAGAATGAGCAGCCATCAGTACTTGTGCTTCAAGCAGCTTTTAGCAAACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

249

Amino Acids

27.01

Weight (kDa)

8.92

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 134 - 176 7.1e-07 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49320
fragaria_vesca FvH4_4g19280
malus_domestica MD13G1158800.v1.1 MD16G1159000.v1.1
prunus_persica Prupe.1G125800_v2.0.a1
pyrus_communis pycom13g13570 pycom16g13400
rosa_chinensis RchiOBHm_Chr4g0424271
rosa_laevigata RLG00000007491
rosa_multiflora Rmu_sc0002260.1_g000024
rosa_roxburghii Rroxscaffold_5G00366210
rosa_rugosa Rorug04G0194900
rosa_samantha Rh4AG252200 Rh4BG257700 Rh4CG268300 Rh4DG252000
rosa_wichuraiana Rw4G021750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 59
AciI CCGC 5 cut(s) 46, 57, 399, 500, 618
AcsI RAATTY 1 cut(s) 573
AcuI CTGAAG 1 cut(s) 321
AfaI GTAC 3 cut(s) 181, 624, 717
AfiI CCNNNNNNNGG 1 cut(s) 669
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 3 cut(s) 388, 442, 728
AluBI AGCT 2 cut(s) 371, 734
AluI AGCT 2 cut(s) 371, 734
Alw21I GWGCWC 1 cut(s) 612
AlwNI CAGNNNCTG 1 cut(s) 374
Ama87I CYCGRG 2 cut(s) 156, 611
AoxI GGCC 5 cut(s) 76, 109, 433, 455, 615
ApeKI GCWGC 6 cut(s) 66, 174, 254, 536, 706, 731
ApoI RAATTY 1 cut(s) 573
AsuHPI GGTGA 2 cut(s) 30, 639
AvaI CYCGRG 2 cut(s) 156, 611
BarI GAAGNNNNNNTAC 2 cut(s) 708, 740
Bbv12I GWGCWC 1 cut(s) 612
BbvI GCAGC 6 cut(s) 78, 186, 266, 523, 718, 743
BccI CCATC 2 cut(s) 185, 718
BceAI ACGGC 1 cut(s) 570
BciVI GTATCC 1 cut(s) 296
BclI TGATCA 1 cut(s) 477
BfaI CTAG 1 cut(s) 453
BfuI GTATCC 1 cut(s) 296
BglII AGATCT 1 cut(s) 31
BlpI GCTNAGC 1 cut(s) 170
BmcAI AGTACT 2 cut(s) 181, 717
BmeT110I CYCGRG 2 cut(s) 156, 611
BmiI GGNNCC 1 cut(s) 357
BmrI ACTGGG 2 cut(s) 457, 459
BmsI GCATC 1 cut(s) 641
BmuI ACTGGG 2 cut(s) 457, 459
BplI GAGNNNNNCTC 2 cut(s) 594, 626
Bpu1102I GCTNAGC 1 cut(s) 170
BsaJI CCNNGG 2 cut(s) 641, 668
Bsc4I CCNNNNNNNGG 1 cut(s) 669
Bse1I ACTGG 3 cut(s) 258, 452, 465
BseDI CCNNGG 2 cut(s) 641, 668
BseLI CCNNNNNNNGG 1 cut(s) 669
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 3 cut(s) 258, 452, 465
BseXI GCAGC 6 cut(s) 78, 186, 266, 523, 718, 743
BsgI GTGCAG 2 cut(s) 273, 585
BshFI GGCC 5 cut(s) 78, 111, 435, 457, 617
BsiHKAI GWGCWC 1 cut(s) 612
BsiHKCI CYCGRG 2 cut(s) 156, 611
BsiSI CCGG 1 cut(s) 540
BslI CCNNNNNNNGG 1 cut(s) 669
BsnI GGCC 5 cut(s) 78, 111, 435, 457, 617
BsoBI CYCGRG 2 cut(s) 156, 611
Bsp1286I GDGCHC 1 cut(s) 612
Bsp143I GATC 2 cut(s) 31, 477
Bsp1720I GCTNAGC 1 cut(s) 170
BspACI CCGC 5 cut(s) 46, 57, 399, 500, 618
BspANI GGCC 5 cut(s) 78, 111, 435, 457, 617
BspCNI CTCAG 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 357
BsrBI CCGCTC 1 cut(s) 59
BsrI ACTGG 3 cut(s) 258, 452, 465
BssECI CCNNGG 2 cut(s) 641, 668
BssMI GATC 2 cut(s) 31, 477
BssT1I CCWWGG 2 cut(s) 641, 668
Bst4CI ACNGT 1 cut(s) 526
BstAPI GCANNNNNTGC 1 cut(s) 260
BstC8I GCNNGC 2 cut(s) 396, 564
BstDEI CTNAG 1 cut(s) 170
BstEII GGTNACC 1 cut(s) 645
BstKTI GATC 2 cut(s) 34, 480
BstMBI GATC 2 cut(s) 31, 477
BstMWI GCNNNNNNNGC 5 cut(s) 75, 117, 260, 563, 607
BstNSI RCATGY 3 cut(s) 29, 310, 398
BstPI GGTNACC 1 cut(s) 645
BstV1I GCAGC 6 cut(s) 78, 186, 266, 523, 718, 743
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BsuI GTATCC 1 cut(s) 296
BsuRI GGCC 5 cut(s) 78, 111, 435, 457, 617
BtsIMutI CAGTG 1 cut(s) 265
Cac8I GCNNGC 2 cut(s) 396, 564
CaiI CAGNNNCTG 1 cut(s) 374
Csp6I GTAC 3 cut(s) 180, 623, 716
CspCI CAANNNNNGTGG 2 cut(s) 405, 440
CviAII CATG 8 cut(s) 26, 165, 286, 299, 307, 351, 362, 395
CviQI GTAC 3 cut(s) 180, 623, 716
DdeI CTNAG 1 cut(s) 170
DpnI GATC 2 cut(s) 33, 479
DpnII GATC 2 cut(s) 31, 477
Eco130I CCWWGG 2 cut(s) 641, 668
Eco147I AGGCCT 1 cut(s) 78
Eco57I CTGAAG 1 cut(s) 321
Eco88I CYCGRG 2 cut(s) 156, 611
Eco91I GGTNACC 1 cut(s) 645
EcoO65I GGTNACC 1 cut(s) 645
EcoT14I CCWWGG 2 cut(s) 641, 668
EcoT22I ATGCAT 1 cut(s) 634
ErhI CCWWGG 2 cut(s) 641, 668
FaeI CATG 8 cut(s) 29, 168, 289, 302, 310, 354, 365, 398
FatI CATG 8 cut(s) 25, 164, 285, 298, 306, 350, 361, 394
FbaI TGATCA 1 cut(s) 477
FspBI CTAG 1 cut(s) 453
HaeIII GGCC 5 cut(s) 78, 111, 435, 457, 617
HapII CCGG 1 cut(s) 540
Hin1II CATG 8 cut(s) 29, 168, 289, 302, 310, 354, 365, 398
HinfI GANTC 2 cut(s) 365, 428
HpaII CCGG 1 cut(s) 540
HphI GGTGA 2 cut(s) 30, 639
Hpy188I TCNGA 2 cut(s) 482, 689
Hpy188III TCNNGA 1 cut(s) 94
Hpy99I CGWCG 1 cut(s) 416
HpyAV CCTTC 4 cut(s) 89, 190, 345, 479
HpyCH4III ACNGT 1 cut(s) 526
HpyCH4V TGCA 7 cut(s) 29, 140, 254, 263, 317, 566, 632
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 117, 260, 563, 607
HpyF3I CTNAG 1 cut(s) 170
Hsp92II CATG 8 cut(s) 29, 168, 289, 302, 310, 354, 365, 398
Ksp22I TGATCA 1 cut(s) 477
Kzo9I GATC 2 cut(s) 31, 477
LmnI GCTCC 2 cut(s) 295, 355
Lsp1109I GCAGC 6 cut(s) 78, 186, 266, 523, 718, 743
LweI GCATC 1 cut(s) 641
MaeI CTAG 1 cut(s) 453
MaeIII GTNAC 2 cut(s) 19, 645
MalI GATC 2 cut(s) 33, 479
MbiI CCGCTC 1 cut(s) 59
MboI GATC 2 cut(s) 31, 477
MboII GAAGA 3 cut(s) 74, 200, 400
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 1 cut(s) 612
MluCI AATT 4 cut(s) 135, 279, 318, 573
MlyI GAGTC 1 cut(s) 437
MnlI CCTC 5 cut(s) 122, 194, 598, 607, 660
Mph1103I ATGCAT 1 cut(s) 634
MseI TTAA 1 cut(s) 248
MslI CAYNNNNRTG 1 cut(s) 290
MspA1I CMGCKG 1 cut(s) 620
MspI CCGG 1 cut(s) 540
MwoI GCNNNNNNNGC 5 cut(s) 75, 117, 260, 563, 607
NdeII GATC 2 cut(s) 31, 477
NlaIII CATG 8 cut(s) 29, 168, 289, 302, 310, 354, 365, 398
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 2 cut(s) 19, 645
NsiI ATGCAT 1 cut(s) 634
NspI RCATGY 3 cut(s) 29, 310, 398
PaeI GCATGC 1 cut(s) 398
PaeR7I CTCGAG 1 cut(s) 611
PceI AGGCCT 1 cut(s) 78
PciI ACATGT 1 cut(s) 306
PfeI GAWTC 1 cut(s) 365
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
PscI ACATGT 1 cut(s) 306
PspEI GGTNACC 1 cut(s) 645
PspN4I GGNNCC 1 cut(s) 357
PspXI VCTCGAGB 1 cut(s) 611
PstNI CAGNNNCTG 1 cut(s) 374
PsuI RGATCY 1 cut(s) 31
RsaI GTAC 3 cut(s) 181, 624, 717
RsaNI GTAC 3 cut(s) 180, 623, 716
RseI CAYNNNNRTG 1 cut(s) 290
SaqAI TTAA 1 cut(s) 248
Sau3AI GATC 2 cut(s) 31, 477
ScaI AGTACT 2 cut(s) 181, 717
SchI GAGTC 1 cut(s) 437
SduI GDGCHC 1 cut(s) 612
SetI ASST 9 cut(s) 244, 337, 373, 490, 552, 609, 647, 652, 736
SfaNI GCATC 1 cut(s) 641
Sfr274I CTCGAG 1 cut(s) 611
SlaI CTCGAG 1 cut(s) 611
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
SphI GCATGC 1 cut(s) 398
Sse9I AATT 4 cut(s) 135, 279, 318, 573
SseBI AGGCCT 1 cut(s) 78
SsiI CCGC 5 cut(s) 46, 57, 399, 500, 618
SspMI CTAG 1 cut(s) 453
StuI AGGCCT 1 cut(s) 78
StyI CCWWGG 2 cut(s) 641, 668
TaaI ACNGT 1 cut(s) 526
TaqI TCGA 1 cut(s) 612
TasI AATT 4 cut(s) 135, 279, 318, 573
TatI WGTACW 3 cut(s) 179, 622, 715
TauI GCSGC 4 cut(s) 48, 401, 503, 620
TfiI GAWTC 1 cut(s) 365
Tru1I TTAA 1 cut(s) 248
Tru9I TTAA 1 cut(s) 248
TscAI CASTG 1 cut(s) 265
TseFI GTSAC 2 cut(s) 19, 645
TseI GCWGC 6 cut(s) 66, 174, 254, 536, 706, 731
Tsp45I GTSAC 2 cut(s) 19, 645
TspDTI ATGAA 3 cut(s) 112, 153, 201
TspRI CASTG 1 cut(s) 265
XapI RAATTY 1 cut(s) 573
XceI RCATGY 3 cut(s) 29, 310, 398
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XhoI CTCGAG 1 cut(s) 611
XspI CTAG 1 cut(s) 453
ZrmI AGTACT 2 cut(s) 181, 717
Zsp2I ATGCAT 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.