Rorug04G0194900

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014135
Physical Location & Seq
Forward (+)
33924293 .. 33926154
1862 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0194900.1

Sequence Viewer

Length: 1377 bp
ATGGTGAACACCATGGAGATCAGTATCATTTCCAGAGACACCATCAAACCATCACTCGATTCCTCTTCACTTAACCATCTAAGTCCTTTCAAACTTTCCCTCGTCGACCAGCTGACTCCCGCAACGTATATGCCACTGATCTTGTTCTACCCCGTTAGTGATCCCAAGTCATCGTTCAATCTCCTCGAAACCCTAACCCGCTTAAAAAGCTCCCTCTCCGAGACTCTCACTCTCTACTATCCATTTTCGGGACGGGCTAAAAACAACCTCGACATTCATGACTTTGACACCGGTATTCCCTTCCTTGAAGCCCGAGTCAATTGTCGCATGCTCGAGTTTCTTAAGCTCCAAGAAACTGAGTTGTTGAACCTTTTCGTTCCATTCCATCCCATTTGCAAGGAAACTGGTGATGAGTCTCTGCTCCCCATGATAGCTTTTCAAGTGAATGTCTTTGCTTGTGGTGGAATAGCAATTGGGGGATCCGCTTCTCACAAGCTATGTGATGGGTTGGCCGCAAACTCTATCCTTGAGTCTTGGTCTACTATTTTTCGTGGGGAGTGCGACAAAATAATACACCCTAATTTCTCCCAAGCCTCATCGCTCTTTCCTCCCAGAGCTCTGTTGCCTAAAAAGTATCTAGATTTAGCAGATAGCTTTTGGTTCAAGGAAAACAATTATGTTACAAGGAGATTTGTGTTTAGTGTCAAAGCCATAGCCACACTACAAGAAAAATCGAAGAGCAAATGCGTGCCCAAGCCATCACGTAATGACGCACTCACATGTTTTATTTGGAAACATGCAATGGCTTCTTCTCGGGCACTGTCACCAGGTTCCTCACCAAGAACTTCCATAGTAGCGCATGCAATGAACATGAGATCACGGATGAAGCCACAAACGTCGTCAGCTAATATCATTGGAAATTTCTTTTGGTGGGCGACAACAGTCGCGGACTCATCTAAGCTAGGAGAAGATCATGAGTTATGTGACTTGGTGCACCTGATTAATGAATCTCACAAAGGATTTGATAGGGGTTATTTTGAGTCTCTTCAGGGAGAAGAGGGAATTGGAGCCATTTCAAACTACTTTAATCAACTAGAAGTCTTGCTTTCTTCAAAGTCCCCACCAGAACTTTATTTATTCACCAATTGGACAAGCATTTTTCACAAATTGGATTTTGGATGGGGGAAGCCTTTCTGGGTAGGGCTCATGGGGAAAGTCGGGCCTGAGTTTAGGAATTTCACGGTGTTCATGGAAGCACAATGTGGTAAAGGAATTGAAGCGTGGGTGACATTGGAGGCAAAGCAAATGGCTCTGCTCCAAAAAGACCTTAAGTTTCTAGCATTTGCTTCTCCAAATTCTGGGATTTCAAGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

458

Amino Acids

51.29

Weight (kDa)

7.59

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 444 2.2e-66 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49320
fragaria_vesca FvH4_4g19280
malus_domestica MD13G1158800.v1.1 MD16G1159000.v1.1
prunus_persica Prupe.1G125800_v2.0.a1
pyrus_communis pycom13g13570 pycom16g13400
rosa_chinensis RchiOBHm_Chr4g0424271
rosa_laevigata RLG00000007491
rosa_multiflora Rmu_sc0002260.1_g000024
rosa_roxburghii Rroxscaffold_5G00366210
rosa_rugosa Rorug04G0194900
rosa_samantha Rh4AG252200 Rh4BG257700 Rh4CG268300 Rh4DG252000
rosa_wichuraiana Rw4G021750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1358
AccI GTMKAC 2 cut(s) 105, 539
AccII CGCG 1 cut(s) 947
AciI CCGC 5 cut(s) 120, 199, 483, 513, 947
AclWI GGATC 3 cut(s) 155, 474, 487
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 3 cut(s) 919, 1234, 1354
AcuI CTGAAG 1 cut(s) 1031
AdeI CACNNNGTG 1 cut(s) 1262
AfiI CCNNNNNNNGG 2 cut(s) 248, 1358
AflII CTTAAG 2 cut(s) 341, 1328
AflIII ACRYGT 1 cut(s) 779
AgeI ACCGGT 1 cut(s) 290
AjnI CCWGG 1 cut(s) 826
AjuI GAANNNNNNNTTGG 6 cut(s) 158, 190, 910, 942, 1047, 1079
AluBI AGCT 9 cut(s) 112, 210, 346, 434, 496, 617, 654, 905, 961
AluI AGCT 9 cut(s) 112, 210, 346, 434, 496, 617, 654, 905, 961
Alw21I GWGCWC 2 cut(s) 619, 996
Alw26I GTCTC 4 cut(s) 30, 215, 420, 1047
Alw44I GTGCAC 1 cut(s) 992
AlwI GGATC 3 cut(s) 155, 474, 487
Ama87I CYCGRG 3 cut(s) 312, 332, 813
AoxI GGCC 2 cut(s) 510, 1220
ApaLI GTGCAC 1 cut(s) 992
ApoI RAATTY 3 cut(s) 919, 1234, 1354
AseI ATTAAT 1 cut(s) 1002
AsiGI ACCGGT 1 cut(s) 290
Asp700I GAANNNNTTC 2 cut(s) 371, 1190
AspLEI GCGC 1 cut(s) 859
AspS9I GGNCC 1 cut(s) 1220
AsuHPI GGTGA 6 cut(s) 16, 419, 816, 828, 1132, 1297
AvaI CYCGRG 3 cut(s) 312, 332, 813
BaeGI GKGCMC 3 cut(s) 753, 820, 996
BamHI GGATCC 1 cut(s) 479
BanII GRGCYC 2 cut(s) 619, 1206
Bbv12I GWGCWC 2 cut(s) 619, 996
BccI CCATC 7 cut(s) 50, 58, 84, 393, 497, 766, 1173
BciT130I CCWGG 1 cut(s) 828
BcoDI GTCTC 4 cut(s) 30, 215, 420, 1047
BfaI CTAG 4 cut(s) 638, 962, 1094, 1337
BfrI CTTAAG 2 cut(s) 341, 1328
BisI GCNGC 1 cut(s) 513
BlsI GCNGC 1 cut(s) 514
Bme1390I CCNGG 1 cut(s) 828
BmeT110I CYCGRG 3 cut(s) 312, 332, 813
BmgT120I GGNCC 1 cut(s) 1220
BmiI GGNNCC 3 cut(s) 481, 832, 1069
BmrFI CCNGG 1 cut(s) 828
BoxI GACNNNNGTC 1 cut(s) 941
BpuEI CTTGAG 1 cut(s) 548
BsaAI YACGTR 1 cut(s) 764
BsaBI GATNNNNATC 1 cut(s) 23
BsaJI CCNNGG 1 cut(s) 12
BsaWI WCCGGW 1 cut(s) 290
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 1358
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 409
Bse3DI GCAATG 2 cut(s) 807, 870
Bse8I GATNNNNATC 1 cut(s) 23
BseBI CCWGG 1 cut(s) 828
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 3 cut(s) 385, 888, 1184
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 2 cut(s) 248, 1358
BseMI GCAATG 2 cut(s) 807, 870
BseMII CTCAG 2 cut(s) 348, 1215
BseNI ACTGG 1 cut(s) 409
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 3 cut(s) 753, 820, 996
Bsh1236I CGCG 1 cut(s) 947
BshFI GGCC 2 cut(s) 512, 1222
BshTI ACCGGT 1 cut(s) 290
BsiHKAI GWGCWC 2 cut(s) 619, 996
BsiHKCI CYCGRG 3 cut(s) 312, 332, 813
BsiSI CCGG 1 cut(s) 291
BslFI GGGAC 2 cut(s) 264, 1102
BslI CCNNNNNNNGG 2 cut(s) 248, 1358
BsmAI GTCTC 4 cut(s) 30, 215, 420, 1047
BsmFI GGGAC 2 cut(s) 264, 1102
BsnI GGCC 2 cut(s) 512, 1222
BsoBI CYCGRG 3 cut(s) 312, 332, 813
Bsp1286I GDGCHC 5 cut(s) 619, 753, 820, 996, 1206
Bsp143I GATC 6 cut(s) 18, 138, 160, 479, 875, 970
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 5 cut(s) 120, 199, 483, 513, 947
BspANI GGCC 2 cut(s) 512, 1222
BspCNI CTCAG 2 cut(s) 349, 1216
BspFNI CGCG 1 cut(s) 947
BspHI TCATGA 2 cut(s) 277, 973
BspLI GGNNCC 3 cut(s) 481, 832, 1069
BspPI GGATC 3 cut(s) 155, 474, 487
BspQI GCTCTTC 1 cut(s) 731
BspTI CTTAAG 2 cut(s) 341, 1328
BsrDI GCAATG 2 cut(s) 807, 870
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 1 cut(s) 409
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 6 cut(s) 18, 138, 160, 479, 875, 970
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 828
Bst4CI ACNGT 3 cut(s) 822, 943, 1243
Bst6I CTCTTC 4 cut(s) 70, 731, 1050, 1050
BstAFI CTTAAG 2 cut(s) 341, 1328
BstBAI YACGTR 1 cut(s) 764
BstC8I GCNNGC 3 cut(s) 329, 749, 861
BstDEI CTNAG 4 cut(s) 80, 357, 957, 1224
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 3 cut(s) 385, 888, 1184
BstFNI CGCG 1 cut(s) 947
BstHHI GCGC 1 cut(s) 859
BstKTI GATC 6 cut(s) 21, 141, 163, 482, 878, 973
BstMAI GTCTC 4 cut(s) 30, 215, 420, 1047
BstMBI GATC 6 cut(s) 18, 138, 160, 479, 875, 970
BstMWI GCNNNNNNNGC 1 cut(s) 207
BstNI CCWGG 1 cut(s) 828
BstNSI RCATGY 5 cut(s) 331, 783, 800, 863, 1375
BstPAI GACNNNNGTC 1 cut(s) 941
BstSCI CCNGG 1 cut(s) 826
BstSLI GKGCMC 3 cut(s) 753, 820, 996
BstUI CGCG 1 cut(s) 947
BstX2I RGATCY 1 cut(s) 479
BstYI RGATCY 1 cut(s) 479
BsuRI GGCC 2 cut(s) 512, 1222
BtgI CCRYGG 1 cut(s) 12
BtgZI GCGATG 1 cut(s) 582
BtsCI GGATG 3 cut(s) 385, 888, 1184
BtsIMutI CAGTG 2 cut(s) 134, 818
Cac8I GCNNGC 3 cut(s) 329, 749, 861
CciI TCATGA 2 cut(s) 277, 973
CfoI GCGC 1 cut(s) 859
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 1220
CseI GACGC 1 cut(s) 779
CsiI ACCWGGT 1 cut(s) 826
CspAI ACCGGT 1 cut(s) 290
CspCI CAANNNNNGTGG 2 cut(s) 123, 158
DdeI CTNAG 4 cut(s) 80, 357, 957, 1224
DpnI GATC 6 cut(s) 20, 140, 162, 481, 877, 972
DpnII GATC 6 cut(s) 18, 138, 160, 479, 875, 970
DraIII CACNNNGTG 1 cut(s) 1262
EaeI YGGCCR 1 cut(s) 510
Eam1104I CTCTTC 4 cut(s) 70, 731, 1050, 1050
EarI CTCTTC 4 cut(s) 70, 731, 1050, 1050
Ecl136II GAGCTC 1 cut(s) 617
Eco130I CCWWGG 1 cut(s) 12
Eco24I GRGCYC 2 cut(s) 619, 1206
Eco53kI GAGCTC 1 cut(s) 617
Eco57I CTGAAG 1 cut(s) 1031
Eco88I CYCGRG 3 cut(s) 312, 332, 813
EcoICRI GAGCTC 1 cut(s) 617
EcoRII CCWGG 1 cut(s) 826
EcoT14I CCWWGG 1 cut(s) 12
EcoT38I GRGCYC 2 cut(s) 619, 1206
ErhI CCWWGG 1 cut(s) 12
FalI AAGNNNNNCTT 2 cut(s) 1089, 1121
FaqI GGGAC 2 cut(s) 264, 1102
FauI CCCGC 2 cut(s) 127, 206
FblI GTMKAC 2 cut(s) 105, 539
Fnu4HI GCNGC 1 cut(s) 513
FokI GGATG 3 cut(s) 372, 895, 1191
FriOI GRGCYC 2 cut(s) 619, 1206
Fsp4HI GCNGC 1 cut(s) 513
FspBI CTAG 4 cut(s) 638, 962, 1094, 1337
GlaI GCGC 1 cut(s) 858
GluI GCNGC 1 cut(s) 513
HaeIII GGCC 2 cut(s) 512, 1222
HapII CCGG 1 cut(s) 291
HgaI GACGC 1 cut(s) 779
HhaI GCGC 1 cut(s) 859
Hin6I GCGC 1 cut(s) 857
HinP1I GCGC 1 cut(s) 857
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 9 cut(s) 59, 115, 223, 315, 413, 530, 950, 1007, 1040
HpaII CCGG 1 cut(s) 291
HphI GGTGA 6 cut(s) 16, 419, 816, 828, 1132, 1297
Hpy166II GTNNAC 4 cut(s) 7, 106, 540, 994
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 5 cut(s) 33, 249, 278, 638, 974
Hpy8I GTNNAC 4 cut(s) 7, 106, 540, 994
Hpy99I CGWCG 2 cut(s) 107, 901
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 3 cut(s) 822, 943, 1243
HpyCH4IV ACGT 3 cut(s) 125, 763, 896
HpyCH4V TGCA 4 cut(s) 396, 800, 863, 994
HpyF10VI GCNNNNNNNGC 1 cut(s) 207
HpyF3I CTNAG 4 cut(s) 80, 357, 957, 1224
HpySE526I ACGT 3 cut(s) 125, 763, 896
HspAI GCGC 1 cut(s) 857
Kzo9I GATC 6 cut(s) 18, 138, 160, 479, 875, 970
LguI GCTCTTC 1 cut(s) 731
LmnI GCTCC 5 cut(s) 215, 351, 426, 1067, 1320
MabI ACCWGGT 1 cut(s) 826
MaeI CTAG 4 cut(s) 638, 962, 1094, 1337
MaeII ACGT 3 cut(s) 125, 763, 896
MaeIII GTNAC 4 cut(s) 679, 822, 983, 1285
MalI GATC 6 cut(s) 20, 140, 162, 481, 877, 972
MboI GATC 6 cut(s) 18, 138, 160, 479, 875, 970
MboII GAAGA 7 cut(s) 57, 748, 801, 980, 1037, 1067, 1101
MfeI CAATTG 3 cut(s) 319, 471, 1144
MflI RGATCY 1 cut(s) 479
MhlI GDGCHC 5 cut(s) 619, 753, 820, 996, 1206
MlyI GAGTC 7 cut(s) 109, 217, 324, 422, 539, 944, 1049
MroXI GAANNNNTTC 2 cut(s) 371, 1190
MseI TTAA 6 cut(s) 72, 203, 342, 1002, 1086, 1329
MslI CAYNNNNRTG 1 cut(s) 778
MspA1I CMGCKG 1 cut(s) 112
MspCI CTTAAG 2 cut(s) 341, 1328
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 1 cut(s) 828
MunI CAATTG 3 cut(s) 319, 471, 1144
MvaI CCWGG 1 cut(s) 828
MvnI CGCG 1 cut(s) 947
MwoI GCNNNNNNNGC 1 cut(s) 207
NcoI CCATGG 1 cut(s) 12
NdeII GATC 6 cut(s) 18, 138, 160, 479, 875, 970
NlaIV GGNNCC 3 cut(s) 481, 832, 1069
NmuCI GTSAC 3 cut(s) 822, 983, 1285
NspI RCATGY 5 cut(s) 331, 783, 800, 863, 1375
PaeI GCATGC 2 cut(s) 331, 863
PaeR7I CTCGAG 1 cut(s) 332
PagI TCATGA 2 cut(s) 277, 973
PciI ACATGT 1 cut(s) 779
PciSI GCTCTTC 1 cut(s) 731
PdmI GAANNNNTTC 2 cut(s) 371, 1190
PfeI GAWTC 2 cut(s) 59, 1007
PflMI CCANNNNNTGG 1 cut(s) 1358
PinAI ACCGGT 1 cut(s) 290
PkrI GCNGC 1 cut(s) 514
PleI GAGTC 7 cut(s) 109, 217, 323, 421, 538, 944, 1048
PpsI GAGTC 7 cut(s) 109, 217, 323, 421, 538, 944, 1048
Ppu21I YACGTR 1 cut(s) 764
PscI ACATGT 1 cut(s) 779
PshAI GACNNNNGTC 1 cut(s) 941
PshBI ATTAAT 1 cut(s) 1002
Psp124BI GAGCTC 1 cut(s) 619
Psp6I CCWGG 1 cut(s) 826
PspGI CCWGG 1 cut(s) 826
PspN4I GGNNCC 3 cut(s) 481, 832, 1069
PspPI GGNCC 1 cut(s) 1220
PspXI VCTCGAGB 1 cut(s) 332
PsuI RGATCY 1 cut(s) 479
PvuII CAGCTG 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 778
SacI GAGCTC 1 cut(s) 619
SalI GTCGAC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 731
SaqAI TTAA 6 cut(s) 72, 203, 342, 1002, 1086, 1329
SatI GCNGC 1 cut(s) 513
Sau3AI GATC 6 cut(s) 18, 138, 160, 479, 875, 970
Sau96I GGNCC 1 cut(s) 1220
SchI GAGTC 7 cut(s) 109, 217, 324, 422, 539, 944, 1049
ScrFI CCNGG 1 cut(s) 828
SduI GDGCHC 5 cut(s) 619, 753, 820, 996, 1206
SexAI ACCWGGT 1 cut(s) 826
Sfr274I CTCGAG 1 cut(s) 332
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 778
SmlI CTYRAG 4 cut(s) 332, 341, 527, 1328
SmoI CTYRAG 4 cut(s) 332, 341, 527, 1328
SphI GCATGC 2 cut(s) 331, 863
SsiI CCGC 5 cut(s) 120, 199, 483, 513, 947
SspMI CTAG 4 cut(s) 638, 962, 1094, 1337
SstI GAGCTC 1 cut(s) 619
StyD4I CCNGG 1 cut(s) 826
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 3 cut(s) 822, 943, 1243
TaiI ACGT 3 cut(s) 128, 766, 899
TaqI TCGA 6 cut(s) 57, 105, 186, 270, 333, 734
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 2 cut(s) 59, 1007
Tru1I TTAA 6 cut(s) 72, 203, 342, 1002, 1086, 1329
Tru9I TTAA 6 cut(s) 72, 203, 342, 1002, 1086, 1329
TscAI CASTG 2 cut(s) 141, 825
TseFI GTSAC 3 cut(s) 822, 983, 1285
Tsp45I GTSAC 3 cut(s) 822, 983, 1285
TspDTI ATGAA 5 cut(s) 266, 881, 899, 1020, 1237
TspGWI ACGGA 1 cut(s) 895
TspRI CASTG 2 cut(s) 141, 825
Van91I CCANNNNNTGG 1 cut(s) 1358
Vha464I CTTAAG 2 cut(s) 341, 1328
VneI GTGCAC 1 cut(s) 992
VspI ATTAAT 1 cut(s) 1002
XapI RAATTY 3 cut(s) 919, 1234, 1354
XbaI TCTAGA 1 cut(s) 637
XceI RCATGY 5 cut(s) 331, 783, 800, 863, 1375
XhoI CTCGAG 1 cut(s) 332
XmiI GTMKAC 2 cut(s) 105, 539
XmnI GAANNNNTTC 2 cut(s) 371, 1190
XspI CTAG 4 cut(s) 638, 962, 1094, 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.