MD13G1158800.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014135
Physical Location & Seq
Forward (+)
12495121 .. 12496739
1619 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1158800.v1.1.491

Sequence Viewer

Length: 798 bp
ATGCCAAAGAGAATGGCAGTCACACATGCAGACCTCTCACCAAGTCGTCCAACCACCGGTTTAGGTAGCAAAACCGGCGCTTTCATCATGGTCTTAACCATTCTCTGCGGCCTCTTCTGCTTCATCCTATCTCTAATCGCTGAAGCTACCCGTTCCAAGGTAACATGGTTGAGCAGTGATGAAGGGGAGGGGGGCGGGGTAAATGAATGTGTATACAGCGGCAGCGGGAAAACGCCGCTGCTATGTGCGACCAGTGCATTTCTGGGACTTTCGGTTATCATGGTGGTGGAACATGGATACATGTTGTTTGCAATTAGTAACTCACCTCCAGCAGCCTTGGTTTTTTGGGAGCCTAATTATTCTGGTCCTGCAAAGAGCTTGAAATGGCAAGCTGCCTTTTTCTTTGTTGCAACTTGGGTTTGTTTTGCTACTGGAGAGATCTTGCTATTGATCGGGGTAAGTGTGGAGTCCGGGCACTTGAGAAAATGGTCCAGACCAAGACCCAGTTGCCTTGTCCTCAGAGACGGCGTGTTATCTGCTGCCGGGGTGTTTGCTTTGACAACAGTTTTCCTTGCTGCTGGGTTATACTTGACTGCATTAAGAGCACAAAGAATCTCCCAACACCAAGAAACTCTGTGGAGAGGGGTGATCGAGGCCTCCGTACTCTATGCATCTCCGCCAACGTCACCTCCTCTCACAACCATTCCTAGGGAGAACCCCTCGTTTAGAGAAAGTCACATTGATCAGCCGCCGTTGATTGTGAATCCGACGGCTCTTAGCAAACAGTTAAATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

266

Amino Acids

28.56

Weight (kDa)

8.78

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 86 - 189 1.9e-17 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49320
fragaria_vesca FvH4_4g19280
malus_domestica MD13G1158800.v1.1 MD16G1159000.v1.1
prunus_persica Prupe.1G125800_v2.0.a1
pyrus_communis pycom13g13570 pycom16g13400
rosa_chinensis RchiOBHm_Chr4g0424271
rosa_laevigata RLG00000007491
rosa_multiflora Rmu_sc0002260.1_g000024
rosa_roxburghii Rroxscaffold_5G00366210
rosa_rugosa Rorug04G0194900
rosa_samantha Rh4AG252200 Rh4BG257700 Rh4CG268300 Rh4DG252000
rosa_wichuraiana Rw4G021750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 213
AciI CCGC 7 cut(s) 108, 195, 219, 225, 236, 677, 749
AcsI RAATTY 1 cut(s) 790
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 1 cut(s) 663
AfiI CCNNNNNNNGG 3 cut(s) 56, 157, 708
AflIII ACRYGT 1 cut(s) 300
AgeI ACCGGT 1 cut(s) 56
AgsI TTSAA 1 cut(s) 382
AluBI AGCT 3 cut(s) 146, 378, 392
AluI AGCT 3 cut(s) 146, 378, 392
Alw21I GWGCWC 1 cut(s) 607
Alw26I GTCTC 1 cut(s) 516
AoxI GGCC 2 cut(s) 109, 654
ApeKI GCWGC 6 cut(s) 222, 238, 332, 392, 539, 575
ApoI RAATTY 1 cut(s) 790
AsiGI ACCGGT 1 cut(s) 56
AspA2I CCTAGG 1 cut(s) 707
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 2 cut(s) 365, 489
AsuC2I CCSGG 2 cut(s) 472, 544
AsuHPI GGTGA 4 cut(s) 30, 315, 658, 678
AvaII GGWCC 2 cut(s) 365, 489
AvrII CCTAGG 1 cut(s) 707
BaeGI GKGCMC 1 cut(s) 477
Bbv12I GWGCWC 1 cut(s) 607
BbvI GCAGC 6 cut(s) 225, 234, 344, 379, 526, 562
BceAI ACGGC 3 cut(s) 541, 736, 786
BciVI GTATCC 1 cut(s) 290
BclI TGATCA 1 cut(s) 742
BcnI CCSGG 2 cut(s) 472, 544
BcoDI GTCTC 1 cut(s) 516
BfaI CTAG 1 cut(s) 708
BfoI RGCGCY 1 cut(s) 81
BfuI GTATCC 1 cut(s) 290
BglII AGATCT 1 cut(s) 438
BlnI CCTAGG 1 cut(s) 707
Bme1390I CCNGG 2 cut(s) 472, 544
Bme18I GGWCC 2 cut(s) 365, 489
BmgT120I GGNCC 2 cut(s) 365, 489
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 2 cut(s) 472, 544
BmrI ACTGGG 1 cut(s) 498
BmsI GCATC 1 cut(s) 680
BmuI ACTGGG 1 cut(s) 498
BplI GAGNNNNNCTC 2 cut(s) 704, 736
BpmI CTGGAG 2 cut(s) 312, 453
BpuEI CTTGAG 1 cut(s) 499
BpuMI CCSGG 2 cut(s) 472, 544
BsaJI CCNNGG 4 cut(s) 156, 336, 543, 707
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 673, 703
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 157, 708
Bse118I RCCGGY 2 cut(s) 56, 74
Bse1I ACTGG 3 cut(s) 252, 436, 504
BseDI CCNNGG 4 cut(s) 156, 336, 543, 707
BseGI GGATG 1 cut(s) 123
BseLI CCNNNNNNNGG 3 cut(s) 56, 157, 708
BseMII CTCAG 1 cut(s) 532
BseNI ACTGG 3 cut(s) 252, 436, 504
BseRI GAGGAG 1 cut(s) 681
BseSI GKGCMC 1 cut(s) 477
BseXI GCAGC 6 cut(s) 225, 234, 344, 379, 526, 562
BseYI CCCAGC 1 cut(s) 578
BshFI GGCC 2 cut(s) 111, 656
BshTI ACCGGT 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 607
BsiSI CCGG 4 cut(s) 57, 75, 471, 543
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 3 cut(s) 56, 157, 708
BsmAI GTCTC 1 cut(s) 516
BsmBI CGTCTC 1 cut(s) 516
BsmFI GGGAC 1 cut(s) 279
BsnI GGCC 2 cut(s) 111, 656
Bsp1286I GDGCHC 2 cut(s) 477, 607
Bsp143I GATC 4 cut(s) 438, 450, 648, 742
BspACI CCGC 7 cut(s) 108, 195, 219, 225, 236, 677, 749
BspANI GGCC 2 cut(s) 111, 656
BspCNI CTCAG 1 cut(s) 531
BspLI GGNNCC 1 cut(s) 351
BsrFI RCCGGY 2 cut(s) 56, 74
BsrI ACTGG 3 cut(s) 252, 436, 504
BssAI RCCGGY 2 cut(s) 56, 74
BssECI CCNNGG 4 cut(s) 156, 336, 543, 707
BssMI GATC 4 cut(s) 438, 450, 648, 742
BssNAI GTATAC 1 cut(s) 214
BssT1I CCWWGG 3 cut(s) 156, 336, 707
Bst1107I GTATAC 1 cut(s) 214
Bst4CI ACNGT 2 cut(s) 565, 786
Bst6I CTCTTC 1 cut(s) 119
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 2 cut(s) 518, 776
BstF5I GGATG 1 cut(s) 123
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 4 cut(s) 441, 453, 651, 745
BstMAI GTCTC 1 cut(s) 516
BstMBI GATC 4 cut(s) 438, 450, 648, 742
BstMWI GCNNNNNNNGC 4 cut(s) 75, 117, 254, 602
BstNSI RCATGY 2 cut(s) 29, 304
BstSCI CCNGG 2 cut(s) 470, 542
BstSLI GKGCMC 1 cut(s) 477
BstV1I GCAGC 6 cut(s) 225, 234, 344, 379, 526, 562
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
BstZ17I GTATAC 1 cut(s) 214
BsuI GTATCC 1 cut(s) 290
BsuRI GGCC 2 cut(s) 111, 656
BtsCI GGATG 1 cut(s) 123
BtsI GCAGTG 1 cut(s) 181
BtsIMutI CAGTG 2 cut(s) 181, 259
Cac8I GCNNGC 1 cut(s) 390
CfoI GCGC 1 cut(s) 80
Cfr10I RCCGGY 2 cut(s) 56, 74
Cfr13I GGNCC 2 cut(s) 365, 489
Csp6I GTAC 1 cut(s) 662
CspAI ACCGGT 1 cut(s) 56
CviAII CATG 6 cut(s) 26, 88, 165, 280, 293, 301
CviJI RGCY 9 cut(s) 111, 146, 335, 352, 378, 392, 656, 748, 773
CviKI_1 RGCY 9 cut(s) 111, 146, 335, 352, 378, 392, 656, 748, 773
CviQI GTAC 1 cut(s) 662
DdeI CTNAG 2 cut(s) 518, 776
DpnI GATC 4 cut(s) 440, 452, 650, 744
DpnII GATC 4 cut(s) 438, 450, 648, 742
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
EciI GGCGGA 1 cut(s) 666
Eco130I CCWWGG 3 cut(s) 156, 336, 707
Eco147I AGGCCT 1 cut(s) 656
Eco47I GGWCC 2 cut(s) 365, 489
Eco57I CTGAAG 1 cut(s) 162
EcoT14I CCWWGG 3 cut(s) 156, 336, 707
EcoT22I ATGCAT 1 cut(s) 673
ErhI CCWWGG 3 cut(s) 156, 336, 707
Esp3I CGTCTC 1 cut(s) 516
FaeI CATG 6 cut(s) 29, 91, 168, 283, 296, 304
FaqI GGGAC 1 cut(s) 279
FatI CATG 6 cut(s) 25, 87, 164, 279, 292, 300
FauI CCCGC 2 cut(s) 188, 218
FbaI TGATCA 1 cut(s) 742
FblI GTMKAC 1 cut(s) 213
FokI GGATG 1 cut(s) 110
FspBI CTAG 1 cut(s) 708
GlaI GCGC 1 cut(s) 79
GsaI CCCAGC 1 cut(s) 582
GsuI CTGGAG 2 cut(s) 312, 453
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 2 cut(s) 111, 656
HapII CCGG 4 cut(s) 57, 75, 471, 543
HhaI GCGC 1 cut(s) 80
Hin1II CATG 6 cut(s) 29, 91, 168, 283, 296, 304
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HinfI GANTC 3 cut(s) 467, 612, 763
HpaII CCGG 4 cut(s) 57, 75, 471, 543
HphI GGTGA 4 cut(s) 30, 315, 658, 678
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 2 cut(s) 521, 768
Hpy188III TCNNGA 1 cut(s) 492
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 1 cut(s) 772
HpyAV CCTTC 1 cut(s) 176
HpyCH4III ACNGT 2 cut(s) 565, 786
HpyCH4IV ACGT 1 cut(s) 683
HpyCH4V TGCA 7 cut(s) 29, 257, 311, 371, 410, 596, 671
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 117, 254, 602
HpyF3I CTNAG 2 cut(s) 518, 776
HpySE526I ACGT 1 cut(s) 683
Hsp92II CATG 6 cut(s) 29, 91, 168, 283, 296, 304
HspAI GCGC 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 742
Kzo9I GATC 4 cut(s) 438, 450, 648, 742
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 6 cut(s) 225, 234, 344, 379, 526, 562
LweI GCATC 1 cut(s) 680
MaeI CTAG 1 cut(s) 708
MaeII ACGT 1 cut(s) 683
MaeIII GTNAC 5 cut(s) 19, 160, 317, 684, 734
MalI GATC 4 cut(s) 440, 452, 650, 744
MboI GATC 4 cut(s) 438, 450, 648, 742
MboII GAAGA 1 cut(s) 106
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 2 cut(s) 477, 607
MluCI AATT 3 cut(s) 312, 355, 790
MlyI GAGTC 1 cut(s) 476
MmeI TCCRAC 2 cut(s) 74, 791
Mph1103I ATGCAT 1 cut(s) 673
MseI TTAA 4 cut(s) 95, 599, 788, 796
MslI CAYNNNNRTG 1 cut(s) 284
MspA1I CMGCKG 3 cut(s) 219, 225, 238
MspI CCGG 4 cut(s) 57, 75, 471, 543
MspR9I CCNGG 2 cut(s) 472, 544
MwoI GCNNNNNNNGC 4 cut(s) 75, 117, 254, 602
NciI CCSGG 2 cut(s) 472, 544
NdeII GATC 4 cut(s) 438, 450, 648, 742
NlaIII CATG 6 cut(s) 29, 91, 168, 283, 296, 304
NlaIV GGNNCC 1 cut(s) 351
NmuCI GTSAC 3 cut(s) 19, 684, 734
NsiI ATGCAT 1 cut(s) 673
NspI RCATGY 2 cut(s) 29, 304
PceI AGGCCT 1 cut(s) 656
PciI ACATGT 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 657
PfeI GAWTC 2 cut(s) 612, 763
PinAI ACCGGT 1 cut(s) 56
PleI GAGTC 1 cut(s) 475
PpsI GAGTC 1 cut(s) 475
PscI ACATGT 1 cut(s) 300
PspFI CCCAGC 1 cut(s) 578
PspN4I GGNNCC 1 cut(s) 351
PspPI GGNCC 2 cut(s) 365, 489
PsuI RGATCY 1 cut(s) 438
RsaI GTAC 1 cut(s) 663
RsaNI GTAC 1 cut(s) 662
RseI CAYNNNNRTG 1 cut(s) 284
SaqAI TTAA 4 cut(s) 95, 599, 788, 796
Sau3AI GATC 4 cut(s) 438, 450, 648, 742
Sau96I GGNCC 2 cut(s) 365, 489
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 2 cut(s) 472, 544
SduI GDGCHC 2 cut(s) 477, 607
SetI ASST 9 cut(s) 36, 67, 148, 162, 328, 380, 394, 686, 691
SfaNI GCATC 1 cut(s) 680
SinI GGWCC 2 cut(s) 365, 489
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 478
SmoI CTYRAG 1 cut(s) 478
Sse9I AATT 3 cut(s) 312, 355, 790
SseBI AGGCCT 1 cut(s) 656
SsiI CCGC 7 cut(s) 108, 195, 219, 225, 236, 677, 749
SspMI CTAG 1 cut(s) 708
StuI AGGCCT 1 cut(s) 656
StyD4I CCNGG 2 cut(s) 470, 542
StyI CCWWGG 3 cut(s) 156, 336, 707
TaaI ACNGT 2 cut(s) 565, 786
TaiI ACGT 1 cut(s) 686
TaqI TCGA 1 cut(s) 651
TasI AATT 3 cut(s) 312, 355, 790
TauI GCSGC 4 cut(s) 111, 222, 238, 751
TfiI GAWTC 2 cut(s) 612, 763
Tru1I TTAA 4 cut(s) 95, 599, 788, 796
Tru9I TTAA 4 cut(s) 95, 599, 788, 796
TscAI CASTG 2 cut(s) 181, 259
TseFI GTSAC 3 cut(s) 19, 684, 734
TseI GCWGC 6 cut(s) 222, 238, 332, 392, 539, 575
Tsp45I GTSAC 3 cut(s) 19, 684, 734
TspDTI ATGAA 4 cut(s) 73, 112, 195, 219
TspGWI ACGGA 1 cut(s) 649
TspRI CASTG 2 cut(s) 181, 259
VpaK11BI GGWCC 2 cut(s) 365, 489
XapI RAATTY 1 cut(s) 790
XceI RCATGY 2 cut(s) 29, 304
XcmI CCANNNNNNNNNTGG 1 cut(s) 259
XmaJI CCTAGG 1 cut(s) 707
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 1 cut(s) 708
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.