Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009405

Physical Location & Seq

Genomic Coordinates Reverse (-)

20111859 .. 20116269

Gene Structure 4411 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G49570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGGTTGCTCGGAAATTTGTCGTCCGCCATGAAGATTCGAGCTTCGATGTCGACTACAACACTGAAGATGGTCTTGAGGTTTTACGATTTCTAATCTTCTCCCTCACTTTAGTTCCTCCTGAAGAACAAAAGATCGTTGCGGAAGATGATAATCGATTGGTGTCTGATGAGTCTGATCTCGCTTCTTTATCTGAGAGACTTCGATTAGTTTCAGTTGGAGAAGATTCTGTAGAGAACTCAGATGCGGAAATGTTGAAATCAGATGAAGAATTGGCTCGAATGTTGCAGGCAGAAGAAGATGCTATTATGTTTCAACAGTTTGTCGCTGCACGAGATAACGGCGAATTCGAAGGGAGAATTAGGCCTTATGTTAGTCAAGTTCTCATGTATGAAGATCCAGTCCGCCAAGATGCTGCTCGAAAGACTGTTCCTAAAGACGAGCTTGAGGAGAAAGCATTGGTTTCTCTGGCCAAGGAAGGGAATTTTGAGCCATCGAAAGAGGAAAGAGACTACGCTTTCCTGCTTCAGCTTCTTTTCTGGTTCAAAAAATCCTTCAGATGGGTCAATGAACCTCCTTGTGACTTTTGTGGTAACAAAACCATAGGCCAGGGGATGGGAAACCCACTTACCTCGGAGCTTGCTTATGGAGCAAATCGAGTTGAAATATATCGCTGTACCATGTGTCCAACAACTACTCGATTCCCTCGGTACAACGATCCGCTAAAGCTCGTAGAAACAAAGAAAGGTCGTTGCGGAGAGTGGGCGAACTGCTTTACGCTATACTGCCGCACGTTTGGCTATGATTCTCGTTTGATAATGGACTTCACAGATCACGTGTGGACAGAATGCTACTCTCACTCGTTAAAGAGGTGGATCCATCTTGACCCTTGTGAAGGAGTGTATGATAAACCCATGTTATATGAGAAAGGATGGAATAAAAAATTGAATTATGTCATTGCGATTTCTAAGGACGGGGTTTGTGATGTTACCAAGCGATATACAAAGAAATGGCATGAGGTTTTATCTAGAAGAACTCTCACAACTGAATCATCTTTGCAAGATGGTCTTCGAACCCTGACAAGGGAACGACGACGTAGCTTAATGTTTGAATCTCTATCTAAGCTTGAATTACGAGACAGAAATGAGCAGGAAGAACTAGAGAGAAATCTGCATTCCGCAGATAATGCCTCAGTTTCTTTACCCGGAAGGCAAAGTGGGGACAGGGAATGGCGTATTATGAGATCAGAATTTGGTTCAGACGAAAACTCATCTGTCAGCTCTTCCTCTTGCCCAGTTCGCAAATGCGTTGATGACCATGTGACCAACATTTATGACTCGTTTCTACCTATCCTTACTCAGTTCGTTGAGGACGGCTTACCTGTAGCAAGAACAAATGAGGTACTTAAGATGATCAAGCAAGTCCTAGTGGATCTGAAGAATGCGCCTTACAAAACAAGGAAGGCTCGATTGACTTTAGATTCAGATAACTCAAGTTCATTCCCGGAGCAGTTTCTTCCCGCACTAGGCGATTTGCTTCTCGCTCTTTCTTTAAAGAGCGAGAGAGACACAAACGGTAAAAGTGTCACAATATCCGTAGACGGGAAACTCACTAAAACTGCTATAGCATTGCCAGTTGCATTGGACGCGTTAAGGGAACTTGTTGCTGACCTCAGCAAATACCAAAACTTAAACAAAGATTCACTGTCTTTTCCACTTGTTAAACAGAACAGGGTATGCTCTGGTTCTGTCCTCGCAAGTGGTGAAGAGCTTCCTTCTGGCATTGCAACCGCAGCTTTTGATGGAATCCAAGAGTCCAAATGGGAAGAACCAAATGGTGCAAAAGGCTGTTGGATCGTGTACAAAACACTCTACAACCAGATGCACCAACTCATAGCATACGAACTCATGTCTGCTAATGATGCCCCAGAGAGAGACCCCAAAGATTGGATTCTTGAAGGAAGTAACGATGGTGGTTCGACATGGTGTGTCCTAGACAAGCAAACTAGTCAGGTATTCGAGGAACGGTTCCAACGCAAATCCTACAAAATAACTACACCTGGATTCCAAGCAAATCTTTTCAGGTTCCGGTTTTTAAGTGTACGAGACGTGAATTCGACCTCGAGACTACAACTAGGGAGCATCGATCTGTACAGAAGTCACCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000224 GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006515 GO:0006516 GO:0006517 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009100 GO:0009636 GO:0009719 GO:0009725 GO:0009751 GO:0009987 GO:0010033 GO:0010035 GO:0010188 GO:0010193 GO:0014070 GO:0016787 GO:0016810 GO:0016811 GO:0019538 GO:0030163 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0050896 GO:0051603 GO:0071704 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Rad4 Transglut_core

Protein Analysis

721

Amino Acids

82.45

Weight (kDa)

5.37

Isoelectric Point (pI)

44.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transglut_core	PF01841	210 - 296	1.3e-16	Transglutaminase-like superfamily
Rad4	PF03835	278 - 357	8.5e-13	Rad4 transglutaminase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009405)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49570
fragaria_vesca	FvH4_6g03130 FvH4_6g03130
malus_domestica	MD12G1238800.v1.1
prunus_persica	Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1
pyrus_communis	pycom12g21950
rosa_chinensis	RchiOBHm_Chr3g0451381
rosa_laevigata	RLG00000025664
rosa_multiflora	Rmu_sc0002575.1_g000005 Rmu_sc0012229.1_g000001
rosa_roxburghii	Rroxscaffold_164G00436540 Rroxscaffold_6G00428800
rosa_rugosa	Rorug02G0633400
rosa_samantha	Rh3AG036400 Rh3BG037000 Rh3CG035600 Rh3DG036500
rosa_wichuraiana	Rw3G002680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	613, 1944
AccI	GTMKAC	2 cut(s)	51, 1596
AccII	CGCG	1 cut(s)	1646
AclWI	GGATC	6 cut(s)	389, 710, 868, 881, 1437, 1859
AcoI	YGGCCR	1 cut(s)	468
AcsI	RAATTY	5 cut(s)	14, 344, 481, 1247, 2110
AcuI	CTGAAG	5 cut(s)	84, 141, 509, 538, 1454
AcvI	CACGTG	1 cut(s)	835
AfaI	GTAC	6 cut(s)	676, 710, 1401, 1859, 2100, 2150
AfiI	CCNNNNNNNGG	9 cut(s)	477, 558, 613, 893, 1080, 1081, 1523, 1599, 1944
AflII	CTTAAG	1 cut(s)	1403
AflIII	ACRYGT	2 cut(s)	834, 1644
AgsI	TTSAA	8 cut(s)	256, 314, 544, 662, 946, 1109, 1127, 1955
AhlI	ACTAGT	1 cut(s)	2003
AjiI	CACGTC	1 cut(s)	2107
AjnI	CCWGG	2 cut(s)	606, 2056
Alw26I	GTCTC	7 cut(s)	190, 501, 1128, 1557, 1926, 2097, 2116
AlwI	GGATC	6 cut(s)	389, 710, 868, 881, 1437, 1859
Ama87I	CYCGRG	1 cut(s)	2119
AoxI	GGCC	3 cut(s)	362, 468, 604
ApeKI	GCWGC	3 cut(s)	326, 413, 1790
ApoI	RAATTY	5 cut(s)	14, 344, 481, 1247, 2110
Asp700I	GAANNNNTTC	1 cut(s)	1767
AspLEI	GCGC	1 cut(s)	1444
AsuC2I	CCSGG	2 cut(s)	1203, 1502
AsuHPI	GGTGA	2 cut(s)	1772, 2150
AsuII	TTCGAA	2 cut(s)	348, 1069
AvaI	CYCGRG	1 cut(s)	2119
BalI	TGGCCA	1 cut(s)	470
BamHI	GGATCC	1 cut(s)	873
BauI	CACGAG	1 cut(s)	330
BbrPI	CACGTG	1 cut(s)	835
BbsI	GAAGAC	1 cut(s)	1058
BbvCI	CCTCAGC	1 cut(s)	1670
BbvI	GCAGC	3 cut(s)	313, 400, 1802
BccI	CCATC	9 cut(s)	62, 499, 552, 607, 885, 924, 1055, 1793, 1961
BceAI	ACGGC	2 cut(s)	355, 1387
BciT130I	CCWGG	2 cut(s)	608, 2058
BclI	TGATCA	1 cut(s)	1410
BcnI	CCSGG	2 cut(s)	1203, 1502
BcoDI	GTCTC	7 cut(s)	190, 501, 1128, 1557, 1926, 2097, 2116
BcuI	ACTAGT	1 cut(s)	2003
BfaI	CTAG	7 cut(s)	1026, 1157, 1424, 1523, 1991, 2004, 2132
BfmI	CTRYAG	3 cut(s)	228, 1380, 1620
BfrI	CTTAAG	1 cut(s)	1403
BisI	GCNGC	4 cut(s)	327, 414, 787, 1791
BlsI	GCNGC	4 cut(s)	328, 415, 788, 1792
Bme1390I	CCNGG	4 cut(s)	608, 1203, 1502, 2058
BmeT110I	CYCGRG	1 cut(s)	2119
BmgBI	CACGTC	1 cut(s)	2107
BmiI	GGNNCC	3 cut(s)	875, 2027, 2084
BmrFI	CCNGG	4 cut(s)	608, 1203, 1502, 2058
BmrI	ACTGGG	1 cut(s)	1286
BmsI	GCATC	6 cut(s)	232, 289, 400, 1869, 1909, 2148
BmuI	ACTGGG	1 cut(s)	1286
BpiI	GAAGAC	1 cut(s)	1058
Bpu10I	CCTNAGC	1 cut(s)	1670
Bpu14I	TTCGAA	2 cut(s)	348, 1069
BpuEI	CTTGAG	3 cut(s)	95, 464, 1474
BpuMI	CCSGG	2 cut(s)	1203, 1502
Bsa29I	ATCGAT	2 cut(s)	154, 2142
BsaAI	YACGTR	1 cut(s)	835
BsaBI	GATNNNNATC	1 cut(s)	150
BsaI	GGTCTC	1 cut(s)	1926
BsaJI	CCNNGG	4 cut(s)	471, 607, 630, 704
BsaWI	WCCGGW	1 cut(s)	2085
Bsc4I	CCNNNNNNNGG	9 cut(s)	477, 558, 613, 893, 1080, 1081, 1523, 1599, 1944
Bse1I	ACTGG	4 cut(s)	398, 1292, 1631, 2161
Bse3DI	GCAATG	3 cut(s)	954, 1625, 1779
Bse8I	GATNNNNATC	1 cut(s)	150
BseBI	CCWGG	2 cut(s)	608, 2058
BseCI	ATCGAT	2 cut(s)	154, 2142
BseDI	CCNNGG	4 cut(s)	471, 607, 630, 704
BseGI	GGATG	2 cut(s)	618, 935
BseJI	GATNNNNATC	1 cut(s)	150
BseLI	CCNNNNNNNGG	9 cut(s)	477, 558, 613, 893, 1080, 1081, 1523, 1599, 1944
BseMI	GCAATG	3 cut(s)	954, 1625, 1779
BseMII	CTCAG	5 cut(s)	183, 252, 1203, 1370, 1684
BseNI	ACTGG	4 cut(s)	398, 1292, 1631, 2161
BseRI	GAGGAG	1 cut(s)	461
BseXI	GCAGC	3 cut(s)	313, 400, 1802
BsgI	GTGCAG	1 cut(s)	312
Bsh1236I	CGCG	1 cut(s)	1646
BshFI	GGCC	3 cut(s)	364, 470, 606
BshVI	ATCGAT	2 cut(s)	154, 2142
BsiHKCI	CYCGRG	1 cut(s)	2119
BsiSI	CCGG	3 cut(s)	1203, 1502, 2086
BslFI	GGGAC	1 cut(s)	1232
BslI	CCNNNNNNNGG	9 cut(s)	477, 558, 613, 893, 1080, 1081, 1523, 1599, 1944
BsmAI	GTCTC	7 cut(s)	190, 501, 1128, 1557, 1926, 2097, 2116
BsmBI	CGTCTC	1 cut(s)	2097
BsmFI	GGGAC	1 cut(s)	1232
BsmI	GAATGC	3 cut(s)	851, 1171, 1444
BsnI	GGCC	3 cut(s)	364, 470, 606
Bso31I	GGTCTC	1 cut(s)	1926
BsoBI	CYCGRG	1 cut(s)	2119
Bsp119I	TTCGAA	2 cut(s)	348, 1069
Bsp1407I	TGTACA	2 cut(s)	1857, 2148
BspANI	GGCC	3 cut(s)	364, 470, 606
BspCNI	CTCAG	5 cut(s)	184, 251, 1202, 1369, 1683
BspDI	ATCGAT	2 cut(s)	154, 2142
BspFNI	CGCG	1 cut(s)	1646
BspLI	GGNNCC	3 cut(s)	875, 2027, 2084
BspPI	GGATC	6 cut(s)	389, 710, 868, 881, 1437, 1859
BspQI	GCTCTTC	2 cut(s)	1285, 1758
BspT104I	TTCGAA	2 cut(s)	348, 1069
BspTI	CTTAAG	1 cut(s)	1403
BspTNI	GGTCTC	1 cut(s)	1926
BsrDI	GCAATG	3 cut(s)	954, 1625, 1779
BsrGI	TGTACA	2 cut(s)	1857, 2148
BsrI	ACTGG	4 cut(s)	398, 1292, 1631, 2161
BssECI	CCNNGG	4 cut(s)	471, 607, 630, 704
BssSI	CACGAG	1 cut(s)	330
BssT1I	CCWWGG	1 cut(s)	471
Bst2BI	CACGAG	1 cut(s)	330
Bst2UI	CCWGG	2 cut(s)	608, 2058
Bst4CI	ACNGT	5 cut(s)	318, 427, 1574, 1704, 2025
Bst6I	CTCTTC	2 cut(s)	1285, 1758
BstAFI	CTTAAG	1 cut(s)	1403
BstAPI	GCANNNNNTGC	1 cut(s)	1184
BstAUI	TGTACA	2 cut(s)	1857, 2148
BstBAI	YACGTR	1 cut(s)	835
BstBI	TTCGAA	2 cut(s)	348, 1069
BstC8I	GCNNGC	2 cut(s)	288, 639
BstDEI	CTNAG	7 cut(s)	192, 238, 966, 1119, 1189, 1356, 1670
BstENI	CCTNNNNNAGG	1 cut(s)	891
BstF5I	GGATG	2 cut(s)	618, 935
BstFNI	CGCG	1 cut(s)	1646
BstHHI	GCGC	1 cut(s)	1444
BstMAI	GTCTC	7 cut(s)	190, 501, 1128, 1557, 1926, 2097, 2116
BstMWI	GCNNNNNNNGC	7 cut(s)	647, 795, 1184, 1296, 1643, 1790, 1919
BstNI	CCWGG	2 cut(s)	608, 2058
BstSCI	CCNGG	4 cut(s)	606, 1201, 1500, 2056
BstSFI	CTRYAG	3 cut(s)	228, 1380, 1620
BstUI	CGCG	1 cut(s)	1646
BstV1I	GCAGC	3 cut(s)	313, 400, 1802
BstV2I	GAAGAC	1 cut(s)	1058
BstX2I	RGATCY	3 cut(s)	394, 873, 1429
BstYI	RGATCY	3 cut(s)	394, 873, 1429
Bsu15I	ATCGAT	2 cut(s)	154, 2142
BsuRI	GGCC	3 cut(s)	364, 470, 606
BsuTUI	ATCGAT	2 cut(s)	154, 2142
BtrI	CACGTC	1 cut(s)	2107
BtsCI	GGATG	2 cut(s)	618, 935
BtsIMutI	CAGTG	2 cut(s)	60, 1700
Cac8I	GCNNGC	2 cut(s)	288, 639
CfoI	GCGC	1 cut(s)	1444
ClaI	ATCGAT	2 cut(s)	154, 2142
CseI	GACGC	1 cut(s)	1652
Csp6I	GTAC	6 cut(s)	675, 709, 1400, 1858, 2099, 2149
CviAII	CATG	8 cut(s)	29, 385, 679, 913, 1013, 1316, 1906, 1980
CviQI	GTAC	6 cut(s)	675, 709, 1400, 1858, 2099, 2149
DdeI	CTNAG	7 cut(s)	192, 238, 966, 1119, 1189, 1356, 1670
DraI	TTTAAA	1 cut(s)	1551
EaeI	YGGCCR	1 cut(s)	468
Eam1104I	CTCTTC	2 cut(s)	1285, 1758
EarI	CTCTTC	2 cut(s)	1285, 1758
EciI	GGCGGA	2 cut(s)	14, 392
Eco130I	CCWWGG	1 cut(s)	471
Eco147I	AGGCCT	1 cut(s)	364
Eco31I	GGTCTC	1 cut(s)	1926
Eco57I	CTGAAG	5 cut(s)	84, 141, 509, 538, 1454
Eco72I	CACGTG	1 cut(s)	835
Eco88I	CYCGRG	1 cut(s)	2119
EcoNI	CCTNNNNNAGG	1 cut(s)	891
EcoRI	GAATTC	2 cut(s)	344, 2110
EcoRII	CCWGG	2 cut(s)	606, 2056
EcoT14I	CCWWGG	1 cut(s)	471
ErhI	CCWWGG	1 cut(s)	471
Esp3I	CGTCTC	1 cut(s)	2097
FaeI	CATG	8 cut(s)	32, 388, 682, 916, 1016, 1319, 1909, 1983
FalI	AAGNNNNNCTT	8 cut(s)	57, 89, 426, 458, 1050, 1082, 2058, 2090
FaqI	GGGAC	1 cut(s)	1232
FatI	CATG	8 cut(s)	28, 384, 678, 912, 1012, 1315, 1905, 1979
FauI	CCCGC	1 cut(s)	1525
FbaI	TGATCA	1 cut(s)	1410
FblI	GTMKAC	2 cut(s)	51, 1596
Fnu4HI	GCNGC	4 cut(s)	327, 414, 787, 1791
FokI	GGATG	2 cut(s)	625, 942
Fsp4HI	GCNGC	4 cut(s)	327, 414, 787, 1791
FspBI	CTAG	7 cut(s)	1026, 1157, 1424, 1523, 1991, 2004, 2132
GlaI	GCGC	1 cut(s)	1443
GluI	GCNGC	4 cut(s)	327, 414, 787, 1791
HaeIII	GGCC	3 cut(s)	364, 470, 606
HapII	CCGG	3 cut(s)	1203, 1502, 2086
HgaI	GACGC	1 cut(s)	1652
HhaI	GCGC	1 cut(s)	1444
Hin1II	CATG	8 cut(s)	32, 388, 682, 916, 1016, 1319, 1909, 1983
Hin6I	GCGC	1 cut(s)	1442
HinP1I	GCGC	1 cut(s)	1442
HincII	GTYRAC	1 cut(s)	52
HindII	GTYRAC	1 cut(s)	52
HindIII	AAGCTT	1 cut(s)	1121
HpaII	CCGG	3 cut(s)	1203, 1502, 2086
HphI	GGTGA	2 cut(s)	1772, 2150
Hpy166II	GTNNAC	5 cut(s)	52, 840, 1597, 1858, 2099
Hpy188III	TCNNGA	6 cut(s)	74, 119, 881, 1026, 1952, 2121
Hpy8I	GTNNAC	5 cut(s)	52, 840, 1597, 1858, 2099
Hpy99I	CGWCG	2 cut(s)	1092, 1095
HpyAV	CCTTC	8 cut(s)	344, 470, 562, 887, 1200, 1453, 1782, 1949
HpyCH4III	ACNGT	5 cut(s)	318, 427, 1574, 1704, 2025
HpyCH4IV	ACGT	4 cut(s)	791, 834, 1093, 2106
HpyCH4V	TGCA	8 cut(s)	286, 329, 1057, 1171, 1637, 1784, 1838, 1882
HpyF10VI	GCNNNNNNNGC	7 cut(s)	647, 795, 1184, 1296, 1643, 1790, 1919
HpyF3I	CTNAG	7 cut(s)	192, 238, 966, 1119, 1189, 1356, 1670
HpySE526I	ACGT	4 cut(s)	791, 834, 1093, 2106
Hsp92II	CATG	8 cut(s)	32, 388, 682, 916, 1016, 1319, 1909, 1983
HspAI	GCGC	1 cut(s)	1442
Ksp22I	TGATCA	1 cut(s)	1410
LguI	GCTCTTC	2 cut(s)	1285, 1758
LmnI	GCTCC	4 cut(s)	634, 647, 1504, 2136
Lsp1109I	GCAGC	3 cut(s)	313, 400, 1802
LweI	GCATC	6 cut(s)	232, 289, 400, 1869, 1909, 2148
MaeI	CTAG	7 cut(s)	1026, 1157, 1424, 1523, 1991, 2004, 2132
MaeII	ACGT	4 cut(s)	791, 834, 1093, 2106
MaeIII	GTNAC	7 cut(s)	578, 590, 985, 1318, 1582, 1961, 2156
MflI	RGATCY	3 cut(s)	394, 873, 1429
MlsI	TGGCCA	1 cut(s)	470
MluI	ACGCGT	1 cut(s)	1644
MluNI	TGGCCA	1 cut(s)	470
MlyI	GAGTC	3 cut(s)	179, 1328, 1820
MmeI	TCCRAC	4 cut(s)	196, 710, 1829, 2053
Mox20I	TGGCCA	1 cut(s)	470
MroXI	GAANNNNTTC	1 cut(s)	1767
MscI	TGGCCA	1 cut(s)	470
MseI	TTAA	8 cut(s)	863, 1100, 1404, 1550, 1649, 1688, 1719, 2093
Msp20I	TGGCCA	1 cut(s)	470
MspCI	CTTAAG	1 cut(s)	1403
MspI	CCGG	3 cut(s)	1203, 1502, 2086
MspR9I	CCNGG	4 cut(s)	608, 1203, 1502, 2058
Mva1269I	GAATGC	3 cut(s)	851, 1171, 1444
MvaI	CCWGG	2 cut(s)	608, 2058
MvnI	CGCG	1 cut(s)	1646
MwoI	GCNNNNNNNGC	7 cut(s)	647, 795, 1184, 1296, 1643, 1790, 1919
NciI	CCSGG	2 cut(s)	1203, 1502
NlaIII	CATG	8 cut(s)	32, 388, 682, 916, 1016, 1319, 1909, 1983
NlaIV	GGNNCC	3 cut(s)	875, 2027, 2084
NmuCI	GTSAC	4 cut(s)	578, 1318, 1582, 2156
NspV	TTCGAA	2 cut(s)	348, 1069
PaeR7I	CTCGAG	1 cut(s)	2119
PceI	AGGCCT	1 cut(s)	364
PciSI	GCTCTTC	2 cut(s)	1285, 1758
PcsI	WCGNNNNNNNCGW	1 cut(s)	345
PctI	GAATGC	3 cut(s)	851, 1171, 1444
PdmI	GAANNNNTTC	1 cut(s)	1767
PflMI	CCANNNNNTGG	2 cut(s)	613, 1944
PfoI	TCCNGGA	1 cut(s)	1500
PkrI	GCNGC	4 cut(s)	328, 415, 788, 1792
PleI	GAGTC	3 cut(s)	178, 1328, 1819
PmaCI	CACGTG	1 cut(s)	835
PmlI	CACGTG	1 cut(s)	835
PpsI	GAGTC	3 cut(s)	178, 1328, 1819
Ppu21I	YACGTR	1 cut(s)	835
Psp6I	CCWGG	2 cut(s)	606, 2056
PspCI	CACGTG	1 cut(s)	835
PspGI	CCWGG	2 cut(s)	606, 2056
PspN4I	GGNNCC	3 cut(s)	875, 2027, 2084
PsuI	RGATCY	3 cut(s)	394, 873, 1429
RsaI	GTAC	6 cut(s)	676, 710, 1401, 1859, 2100, 2150
RsaNI	GTAC	6 cut(s)	675, 709, 1400, 1858, 2099, 2149
SalI	GTCGAC	1 cut(s)	50
SapI	GCTCTTC	2 cut(s)	1285, 1758
SaqAI	TTAA	8 cut(s)	863, 1100, 1404, 1550, 1649, 1688, 1719, 2093
SatI	GCNGC	4 cut(s)	327, 414, 787, 1791
SchI	GAGTC	3 cut(s)	179, 1328, 1820
ScrFI	CCNGG	4 cut(s)	608, 1203, 1502, 2058
SfaNI	GCATC	6 cut(s)	232, 289, 400, 1869, 1909, 2148
SfcI	CTRYAG	3 cut(s)	228, 1380, 1620
Sfr274I	CTCGAG	1 cut(s)	2119
SfuI	TTCGAA	2 cut(s)	348, 1069
SlaI	CTCGAG	1 cut(s)	2119
SmlI	CTYRAG	5 cut(s)	74, 443, 1403, 1489, 2119
SmoI	CTYRAG	5 cut(s)	74, 443, 1403, 1489, 2119
SpeI	ACTAGT	1 cut(s)	2003
SseBI	AGGCCT	1 cut(s)	364
SspMI	CTAG	7 cut(s)	1026, 1157, 1424, 1523, 1991, 2004, 2132
StuI	AGGCCT	1 cut(s)	364
StyD4I	CCNGG	4 cut(s)	606, 1201, 1500, 2056
StyI	CCWWGG	1 cut(s)	471
TaaI	ACNGT	5 cut(s)	318, 427, 1574, 1704, 2025
TaiI	ACGT	4 cut(s)	794, 837, 1096, 2109
TatI	WGTACW	2 cut(s)	1857, 2148
TauI	GCSGC	1 cut(s)	789
Tru1I	TTAA	8 cut(s)	863, 1100, 1404, 1550, 1649, 1688, 1719, 2093
Tru9I	TTAA	8 cut(s)	863, 1100, 1404, 1550, 1649, 1688, 1719, 2093
TscAI	CASTG	2 cut(s)	67, 1707
TseFI	GTSAC	4 cut(s)	578, 1318, 1582, 2156
TseI	GCWGC	3 cut(s)	326, 413, 1790
Tsp45I	GTSAC	4 cut(s)	578, 1318, 1582, 2156
TspDTI	ATGAA	5 cut(s)	45, 279, 405, 582, 1485
TspGWI	ACGGA	1 cut(s)	1582
TspRI	CASTG	2 cut(s)	67, 1707
Van91I	CCANNNNNTGG	2 cut(s)	613, 1944
Vha464I	CTTAAG	1 cut(s)	1403
XagI	CCTNNNNNAGG	1 cut(s)	891
XapI	RAATTY	5 cut(s)	14, 344, 481, 1247, 2110
XbaI	TCTAGA	1 cut(s)	1025
XhoI	CTCGAG	1 cut(s)	2119
XmiI	GTMKAC	2 cut(s)	51, 1596
XmnI	GAANNNNTTC	1 cut(s)	1767
XspI	CTAG	7 cut(s)	1026, 1157, 1424, 1523, 1991, 2004, 2132

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.