Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000164

Orthogroup ID

OG0009405

Physical Location & Seq

Genomic Coordinates Reverse (-)

399416 .. 405183

Gene Structure 5768 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_164G00436540.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2139 bp

ATGGTGGCTCGGAGCTTCCAGGTCCGCCACAACGACTCCGATTTCACCGTCGTCTACGACACCGACGACGGCCTCGAAGTTCTGAAATACCACCAGCTCTTCTCTCTCACGTCTGTACCTCCTGATGAGCAAAAGATCATCGGAGCCGACGACGATCGCGTCGTCTCGGATGATTCGGACCTGGTTGCGATCTCCGAGAAGCTCCGGTTGGTTTCGATCAGCGAAGAGGAACCGGAATCGACGGCCGGGAATGAGGAATTGCTGAAATCCGATGAGGAATTGGCCAGAATGTTGCAGGCGGAGGAGGAGGCGCTTATGTATCAGCAATACGCTGTTCCTGAAGATAATGGAGCATTTGAGCAAAGAGTGCGGCCTTATGTCAGTCAAGTTCTTCTGTATGAGACCGCGGTACGCCAGGAGGCTGCTAGGAAAACAGTCCCTGTAGAACAGCTTGAGGAGAAGGCATTGGTCAGTTTGGCCAAGGAGGGGAACTTAACTCCATCAAAGAATGAACAAGATCATGCGTTCCTGCTGCAGCTGCTTTTCTGGTTCAAACAATCCTTCAGGTGGGTTAATGAACCTCCCTGTGATGGTTGCGGCAAGAAAACCATCAGTGTTGGCATGGATGTTGCACTTCTTCATCAAGATGCACTTCTTGCCCCAGCAGAGACTCGTTTCCCACGCTACAATGATCCACTAAAGCTTCTGGAAACAAGAAGGGGTCGTTGTGGGGAATGGGCAAATTGCTTCACACTTTATTGCCGAGCTTTTGGGTATGAATCACGCCTGATCCTGGATTTCACAGATCATGTTTGGACAGAGTGCTTCTCAGAATCTTTAGGAAGATGGATGCATCTTGATCCTTGTGAAGGAGTGTATGACAAGCCACTGTTATATGAAAGCGGGTGGAATAAGAAATTAAATTATGTACTTGCCATTGGGAAGGATGGAGTATGTGATGTAACTAAACGCTACACAAGGAAGTGGCATGAGGTTCTTTCTCGACGTAACATTATTGCCGAGCCTGCACTGTCATCTGTGCTTGCTAATATAACAAAAGAATGTCGGAGAGGCTTTACTTCTCAAGTGCGTTCTGCACTTGAAGACCGCGATGAGAAGGAAAGACAACAACTTGAGAGAGATTTACATTCTACAGATGATGCCTCAACCTCATTACCTGGAAGACGAAGTGGGGACAAGGAATGGCGCAAGTCAAGAATGGAAGATGGCTCTGATGAGAGTTGCTCCTTAACTGGTTCTTCTTGTCCACTTCGTCAATGTGTGGACGAGCATGTGACAAAAATTTACAGTGGTTTTTTTCCAATTCTTTCACAGTTGGTTAATGAAGGTTTTCCGAAGTCAAGGGCAGTTGAAGTACTGGAGATTCTTAAAGAAATTCTCATAAATCTTAAGAAATCCCCTTTCAAAACAAGGAGGGTGTCAATAGATTCAGTTCCAAATATCAGCCAGTCTGTTGTTCAGCAGTTGTTGCCCTCTTTCACCGAGTTACTCAGCGCTCTTTCATTGAGTAGCAAGGCAGATACTGATGGGAGGGTTGACATTTATTTGGCTGGACCTCCTGTTAAAACCTCTATGGCACTGCCTGTTACATTCCATGCTTTGGACGTCACAATCCGTAATCTCAAGAGTTGTGAAAACTTTGTTAAAGATTCTCTGTGCTTGCCGCTTCTTAAGTTAAACAGAATACATTCCGGTGTAGTCCGTGCAAGTGGAGAAGAAATTCCTTTTGGAATTGCCACATCAGCTTTTGATGGGACACGCATGTCCAAGTGGGAAGAACCAAATGGTGCAGAAGGTTGTTGGATCATGTATAAAGTATCGCATAACCAGATGCACGAACTGGTGGCATATGAGTTGATGTCAGCCAATGATGCACCTGAAAGAGATCCAATGGATTGGGTTGTCGAGGGAAGCAATGATGGGGGGTCAAGCTGGCATTTATTAGATAAACAAACTTCTCAACTATTCGATAGTCGTTTTCAGAGTAGAACATTTAAAGTTCCATCTGAAGGTTTCCTATCAAATGCTTTCAGGTTTAGATTTTTGAGGGTTCGTGATATTCAATCAAATTCTCGGCTGCAATTAGGTAGCATTGATCTCTATTCTAGAAGCAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000224 GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006515 GO:0006516 GO:0006517 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009100 GO:0009636 GO:0009719 GO:0009725 GO:0009751 GO:0009987 GO:0010033 GO:0010035 GO:0010188 GO:0010193 GO:0014070 GO:0016787 GO:0016810 GO:0016811 GO:0019538 GO:0030163 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0050896 GO:0051603 GO:0071704 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Rad4 Transglut_core

Protein Analysis

712

Amino Acids

80.79

Weight (kDa)

5.32

Isoelectric Point (pI)

57.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transglut_core	PF01841	223 - 288	2.1e-15	Transglutaminase-like superfamily
Rad4	PF03835	270 - 378	3e-11	Rad4 transglutaminase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009405)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49570
fragaria_vesca	FvH4_6g03130 FvH4_6g03130
malus_domestica	MD12G1238800.v1.1
prunus_persica	Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1
pyrus_communis	pycom12g21950
rosa_chinensis	RchiOBHm_Chr3g0451381
rosa_laevigata	RLG00000025664
rosa_multiflora	Rmu_sc0002575.1_g000005 Rmu_sc0012229.1_g000001
rosa_roxburghii	Rroxscaffold_164G00436540 Rroxscaffold_6G00428800
rosa_rugosa	Rorug02G0633400
rosa_samantha	Rh3AG036400 Rh3BG037000 Rh3CG035600 Rh3DG036500
rosa_wichuraiana	Rw3G002680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	158, 1783
AatII	GACGTC	1 cut(s)	1629
AccB7I	CCANNNNNTGG	1 cut(s)	1621
AccI	GTMKAC	1 cut(s)	54
AccII	CGCG	3 cut(s)	159, 407, 1110
AciI	CCGC	9 cut(s)	25, 299, 370, 405, 407, 597, 903, 1108, 1685
AclWI	GGATC	5 cut(s)	686, 784, 854, 1832, 1901
AcoI	YGGCCR	3 cut(s)	243, 282, 477
AcsI	RAATTY	4 cut(s)	1302, 1395, 1740, 2089
AcuI	CTGAAG	3 cut(s)	360, 547, 2049
AcyI	GRCGYC	1 cut(s)	1626
AfaI	GTAC	4 cut(s)	117, 411, 930, 1377
AfeI	AGCGCT	1 cut(s)	1516
AfiI	CCNNNNNNNGG	7 cut(s)	486, 567, 590, 793, 869, 1621, 2030
AflII	CTTAAG	2 cut(s)	1409, 1691
AgsI	TTSAA	5 cut(s)	553, 1103, 1373, 1426, 2084
AjiI	CACGTC	1 cut(s)	111
AjnI	CCWGG	5 cut(s)	18, 180, 414, 792, 1177
AjuI	GAANNNNNNNTTGG	4 cut(s)	191, 223, 1731, 1763
AluBI	AGCT	9 cut(s)	15, 97, 202, 451, 538, 703, 767, 1766, 1953
AluI	AGCT	9 cut(s)	15, 97, 202, 451, 538, 703, 767, 1766, 1953
Alw26I	GTCTC	3 cut(s)	169, 395, 662
AlwI	GGATC	5 cut(s)	686, 784, 854, 1832, 1901
AlwNI	CAGNNNCTG	2 cut(s)	440, 1544
Aor51HI	AGCGCT	1 cut(s)	1516
AoxI	GGCC	5 cut(s)	70, 243, 282, 371, 477
ApeKI	GCWGC	5 cut(s)	422, 532, 535, 538, 2098
ApoI	RAATTY	4 cut(s)	1302, 1395, 1740, 2089
Asp700I	GAANNNNTTC	3 cut(s)	1350, 1708, 1740
AspLEI	GCGC	3 cut(s)	313, 1209, 1517
AspS9I	GGNCC	3 cut(s)	22, 178, 1574
AsuC2I	CCSGG	1 cut(s)	247
AsuHPI	GGTGA	2 cut(s)	37, 1492
AvaII	GGWCC	3 cut(s)	22, 178, 1574
BaeI	ACNNNNGTAYC	2 cut(s)	99, 132
BalI	TGGCCA	2 cut(s)	284, 479
BbsI	GAAGAC	2 cut(s)	1110, 1189
BbvI	GCAGC	5 cut(s)	409, 519, 525, 547, 2085
BceAI	ACGGC	2 cut(s)	85, 258
BciT130I	CCWGG	5 cut(s)	20, 182, 416, 794, 1179
BcnI	CCSGG	1 cut(s)	247
BcoDI	GTCTC	3 cut(s)	169, 395, 662
BfaI	CTAG	2 cut(s)	426, 2127
BfmI	CTRYAG	3 cut(s)	441, 533, 1152
BfoI	RGCGCY	2 cut(s)	314, 1518
BfrI	CTTAAG	2 cut(s)	1409, 1691
BisI	GCNGC	8 cut(s)	371, 423, 533, 536, 539, 598, 1685, 2099
BlsI	GCNGC	8 cut(s)	372, 424, 534, 537, 540, 599, 1686, 2100
BmcAI	AGTACT	1 cut(s)	1377
Bme1390I	CCNGG	6 cut(s)	20, 182, 247, 416, 794, 1179
Bme18I	GGWCC	3 cut(s)	22, 178, 1574
BmgBI	CACGTC	1 cut(s)	111
BmgT120I	GGNCC	3 cut(s)	22, 178, 1574
BmiI	GGNNCC	2 cut(s)	145, 231
BmrFI	CCNGG	6 cut(s)	20, 182, 247, 416, 794, 1179
BmsI	GCATC	6 cut(s)	637, 840, 862, 1150, 1842, 1882
BpiI	GAAGAC	2 cut(s)	1110, 1189
BplI	GAGNNNNNCTC	4 cut(s)	812, 844, 1229, 1261
BpmI	CTGGAG	1 cut(s)	1400
BpuEI	CTTGAG	4 cut(s)	473, 1068, 1154, 1628
BpuMI	CCSGG	1 cut(s)	247
BsaHI	GRCGYC	1 cut(s)	1626
BsaI	GGTCTC	1 cut(s)	395
BsaJI	CCNNGG	2 cut(s)	405, 480
BsaWI	WCCGGW	3 cut(s)	204, 232, 1712
BsaXI	ACNNNNNCTCC	2 cut(s)	20, 50
Bsc4I	CCNNNNNNNGG	7 cut(s)	486, 567, 590, 793, 869, 1621, 2030
Bse1I	ACTGG	4 cut(s)	1258, 1383, 1468, 1866
Bse3DI	GCAATG	1 cut(s)	1942
BseBI	CCWGG	5 cut(s)	20, 182, 416, 794, 1179
BseDI	CCNNGG	2 cut(s)	405, 480
BseGI	GGATG	4 cut(s)	175, 631, 855, 952
BseLI	CCNNNNNNNGG	7 cut(s)	486, 567, 590, 793, 869, 1621, 2030
BseMI	GCAATG	1 cut(s)	1942
BseMII	CTCAG	2 cut(s)	843, 1525
BseNI	ACTGG	4 cut(s)	1258, 1383, 1468, 1866
BseRI	GAGGAG	3 cut(s)	317, 320, 470
BseX3I	CGGCCG	1 cut(s)	243
BseXI	GCAGC	5 cut(s)	409, 519, 525, 547, 2085
BseYI	CCCAGC	1 cut(s)	661
BsgI	GTGCAG	3 cut(s)	1011, 1080, 1830
Bsh1236I	CGCG	3 cut(s)	159, 407, 1110
Bsh1285I	CGRYCG	2 cut(s)	157, 246
BshFI	GGCC	5 cut(s)	72, 245, 284, 373, 479
BsiEI	CGRYCG	2 cut(s)	157, 246
BsiSI	CCGG	4 cut(s)	205, 233, 246, 1713
BslFI	GGGAC	3 cut(s)	422, 1208, 1789
BslI	CCNNNNNNNGG	7 cut(s)	486, 567, 590, 793, 869, 1621, 2030
BsmAI	GTCTC	3 cut(s)	169, 395, 662
BsmBI	CGTCTC	1 cut(s)	169
BsmFI	GGGAC	3 cut(s)	422, 1208, 1789
BsnI	GGCC	5 cut(s)	72, 245, 284, 373, 479
Bso31I	GGTCTC	1 cut(s)	395
BspACI	CCGC	9 cut(s)	25, 299, 370, 405, 407, 597, 903, 1108, 1685
BspANI	GGCC	5 cut(s)	72, 245, 284, 373, 479
BspCNI	CTCAG	2 cut(s)	842, 1524
BspFNI	CGCG	3 cut(s)	159, 407, 1110
BspLI	GGNNCC	2 cut(s)	145, 231
BspMAI	CTGCAG	1 cut(s)	537
BspPI	GGATC	5 cut(s)	686, 784, 854, 1832, 1901
BspQI	GCTCTTC	1 cut(s)	104
BspTI	CTTAAG	2 cut(s)	1409, 1691
BspTNI	GGTCTC	1 cut(s)	395
BsrDI	GCAATG	1 cut(s)	1942
BsrI	ACTGG	4 cut(s)	1258, 1383, 1468, 1866
BssECI	CCNNGG	2 cut(s)	405, 480
BssNI	GRCGYC	1 cut(s)	1626
BssT1I	CCWWGG	1 cut(s)	480
Bst2UI	CCWGG	5 cut(s)	20, 182, 416, 794, 1179
Bst4CI	ACNGT	6 cut(s)	49, 436, 891, 1032, 1310, 1335
Bst6I	CTCTTC	2 cut(s)	104, 219
BstACI	GRCGYC	1 cut(s)	1626
BstAFI	CTTAAG	2 cut(s)	1409, 1691
BstAPI	GCANNNNNTGC	3 cut(s)	367, 656, 1489
BstC8I	GCNNGC	5 cut(s)	297, 1026, 1044, 1682, 1955
BstDEI	CTNAG	2 cut(s)	829, 1511
BstDSI	CCRYGG	1 cut(s)	405
BstENI	CCTNNNNNAGG	1 cut(s)	867
BstF5I	GGATG	4 cut(s)	175, 631, 855, 952
BstFNI	CGCG	3 cut(s)	159, 407, 1110
BstH2I	RGCGCY	2 cut(s)	314, 1518
BstHHI	GCGC	3 cut(s)	313, 1209, 1517
BstMAI	GTCTC	3 cut(s)	169, 395, 662
BstMCI	CGRYCG	2 cut(s)	157, 246
BstMWI	GCNNNNNNNGC	7 cut(s)	367, 538, 656, 1025, 1489, 1763, 1892
BstNI	CCWGG	5 cut(s)	20, 182, 416, 794, 1179
BstNSI	RCATGY	2 cut(s)	1295, 1786
BstSCI	CCNGG	6 cut(s)	18, 180, 245, 414, 792, 1177
BstSFI	CTRYAG	3 cut(s)	441, 533, 1152
BstUI	CGCG	3 cut(s)	159, 407, 1110
BstV1I	GCAGC	5 cut(s)	409, 519, 525, 547, 2085
BstV2I	GAAGAC	2 cut(s)	1110, 1189
BstX2I	RGATCY	1 cut(s)	1906
BstXI	CCANNNNNNTGG	1 cut(s)	1917
BstYI	RGATCY	1 cut(s)	1906
BstZI	CGGCCG	1 cut(s)	243
BsuRI	GGCC	5 cut(s)	72, 245, 284, 373, 479
BtgI	CCRYGG	1 cut(s)	405
BtgZI	GCGATG	1 cut(s)	1125
BtrI	CACGTC	1 cut(s)	111
BtsCI	GGATG	4 cut(s)	175, 631, 855, 952
BtsI	GCAGTG	1 cut(s)	1598
BtsIMutI	CAGTG	5 cut(s)	619, 887, 1028, 1315, 1598
Cac8I	GCNNGC	5 cut(s)	297, 1026, 1044, 1682, 1955
CaiI	CAGNNNCTG	2 cut(s)	440, 1544
CfoI	GCGC	3 cut(s)	313, 1209, 1517
Cfr13I	GGNCC	3 cut(s)	22, 178, 1574
Cfr42I	CCGCGG	1 cut(s)	408
CseI	GACGC	1 cut(s)	148
CsiI	ACCWGGT	1 cut(s)	180
Csp6I	GTAC	4 cut(s)	116, 410, 929, 1376
CviAII	CATG	8 cut(s)	521, 622, 809, 989, 1292, 1616, 1783, 1828
CviQI	GTAC	4 cut(s)	116, 410, 929, 1376
DdeI	CTNAG	2 cut(s)	829, 1511
DraI	TTTAAA	1 cut(s)	2017
DrdI	GACNNNNNNGTC	2 cut(s)	158, 1783
DseDI	GACNNNNNNGTC	2 cut(s)	158, 1783
EaeI	YGGCCR	3 cut(s)	243, 282, 477
EagI	CGGCCG	1 cut(s)	243
Eam1104I	CTCTTC	2 cut(s)	104, 219
EarI	CTCTTC	2 cut(s)	104, 219
EciI	GGCGGA	2 cut(s)	14, 314
EclXI	CGGCCG	1 cut(s)	243
Eco130I	CCWWGG	1 cut(s)	480
Eco31I	GGTCTC	1 cut(s)	395
Eco47I	GGWCC	3 cut(s)	22, 178, 1574
Eco47III	AGCGCT	1 cut(s)	1516
Eco52I	CGGCCG	1 cut(s)	243
Eco57I	CTGAAG	3 cut(s)	360, 547, 2049
EcoNI	CCTNNNNNAGG	1 cut(s)	867
EcoRII	CCWGG	5 cut(s)	18, 180, 414, 792, 1177
EcoT14I	CCWWGG	1 cut(s)	480
EcoT22I	ATGCAT	1 cut(s)	855
ErhI	CCWWGG	1 cut(s)	480
Esp3I	CGTCTC	1 cut(s)	169
FaeI	CATG	8 cut(s)	524, 625, 812, 992, 1295, 1619, 1786, 1831
FalI	AAGNNNNNCTT	2 cut(s)	636, 668
FaqI	GGGAC	3 cut(s)	422, 1208, 1789
FatI	CATG	8 cut(s)	520, 621, 808, 988, 1291, 1615, 1782, 1827
FauI	CCCGC	1 cut(s)	896
FauNDI	CATATG	1 cut(s)	1870
FblI	GTMKAC	1 cut(s)	54
Fnu4HI	GCNGC	8 cut(s)	371, 423, 533, 536, 539, 598, 1685, 2099
FokI	GGATG	4 cut(s)	182, 638, 862, 959
Fsp4HI	GCNGC	8 cut(s)	371, 423, 533, 536, 539, 598, 1685, 2099
FspBI	CTAG	2 cut(s)	426, 2127
GlaI	GCGC	3 cut(s)	312, 1208, 1516
GluI	GCNGC	8 cut(s)	371, 423, 533, 536, 539, 598, 1685, 2099
GsaI	CCCAGC	1 cut(s)	665
GsuI	CTGGAG	1 cut(s)	1400
HaeII	RGCGCY	2 cut(s)	314, 1518
HaeIII	GGCC	5 cut(s)	72, 245, 284, 373, 479
HapII	CCGG	4 cut(s)	205, 233, 246, 1713
HgaI	GACGC	1 cut(s)	148
HhaI	GCGC	3 cut(s)	313, 1209, 1517
Hin1I	GRCGYC	1 cut(s)	1626
Hin1II	CATG	8 cut(s)	524, 625, 812, 992, 1295, 1619, 1786, 1831
Hin6I	GCGC	3 cut(s)	311, 1207, 1515
HinP1I	GCGC	3 cut(s)	311, 1207, 1515
HincII	GTYRAC	1 cut(s)	1558
HindII	GTYRAC	1 cut(s)	1558
HindIII	AAGCTT	1 cut(s)	701
HinfI	GANTC	9 cut(s)	35, 173, 236, 670, 779, 833, 1384, 1448, 1670
HpaII	CCGG	4 cut(s)	205, 233, 246, 1713
HphI	GGTGA	2 cut(s)	37, 1492
Hpy166II	GTNNAC	4 cut(s)	55, 1268, 1285, 1558
Hpy8I	GTNNAC	4 cut(s)	55, 1268, 1285, 1558
Hpy99I	CGWCG	8 cut(s)	53, 68, 71, 152, 155, 164, 244, 1008
HpyAV	CCTTC	9 cut(s)	454, 571, 711, 863, 937, 1111, 1340, 1808, 2024
HpyCH4III	ACNGT	6 cut(s)	49, 436, 891, 1032, 1310, 1335
HpyCH4IV	ACGT	3 cut(s)	110, 1006, 1626
HpyF10VI	GCNNNNNNNGC	7 cut(s)	367, 538, 656, 1025, 1489, 1763, 1892
HpyF3I	CTNAG	2 cut(s)	829, 1511
HpySE526I	ACGT	3 cut(s)	110, 1006, 1626
Hsp92I	GRCGYC	1 cut(s)	1626
Hsp92II	CATG	8 cut(s)	524, 625, 812, 992, 1295, 1619, 1786, 1831
HspAI	GCGC	3 cut(s)	311, 1207, 1515
KspI	CCGCGG	1 cut(s)	408
LguI	GCTCTTC	1 cut(s)	104
LmnI	GCTCC	5 cut(s)	12, 143, 207, 350, 1250
Lsp1109I	GCAGC	5 cut(s)	409, 519, 525, 547, 2085
LweI	GCATC	6 cut(s)	637, 840, 862, 1150, 1842, 1882
MabI	ACCWGGT	1 cut(s)	180
MaeI	CTAG	2 cut(s)	426, 2127
MaeII	ACGT	3 cut(s)	110, 1006, 1626
MaeIII	GTNAC	6 cut(s)	961, 1007, 1294, 1506, 1606, 1627
MflI	RGATCY	1 cut(s)	1906
MlsI	TGGCCA	2 cut(s)	284, 479
MluNI	TGGCCA	2 cut(s)	284, 479
MlyI	GAGTC	2 cut(s)	29, 664
MmeI	TCCRAC	2 cut(s)	1046, 1802
Mox20I	TGGCCA	2 cut(s)	284, 479
Mph1103I	ATGCAT	1 cut(s)	855
MroXI	GAANNNNTTC	3 cut(s)	1350, 1708, 1740
MscI	TGGCCA	2 cut(s)	284, 479
MslI	CAYNNNNRTG	2 cut(s)	645, 1713
Msp20I	TGGCCA	2 cut(s)	284, 479
MspA1I	CMGCKG	2 cut(s)	407, 538
MspCI	CTTAAG	2 cut(s)	1409, 1691
MspI	CCGG	4 cut(s)	205, 233, 246, 1713
MspR9I	CCNGG	6 cut(s)	20, 182, 247, 416, 794, 1179
MvaI	CCWGG	5 cut(s)	20, 182, 416, 794, 1179
MvnI	CGCG	3 cut(s)	159, 407, 1110
MwoI	GCNNNNNNNGC	7 cut(s)	367, 538, 656, 1025, 1489, 1763, 1892
NciI	CCSGG	1 cut(s)	247
NdeI	CATATG	1 cut(s)	1870
NlaIII	CATG	8 cut(s)	524, 625, 812, 992, 1295, 1619, 1786, 1831
NlaIV	GGNNCC	2 cut(s)	145, 231
NmeAIII	GCCGAG	3 cut(s)	788, 1045, 2074
NmuCI	GTSAC	2 cut(s)	1294, 1627
NsiI	ATGCAT	1 cut(s)	855
NspI	RCATGY	2 cut(s)	1295, 1786
PciSI	GCTCTTC	1 cut(s)	104
PcsI	WCGNNNNNNNCGW	5 cut(s)	63, 72, 147, 156, 159
PdmI	GAANNNNTTC	3 cut(s)	1350, 1708, 1740
PfeI	GAWTC	7 cut(s)	173, 236, 779, 833, 1384, 1448, 1670
PflMI	CCANNNNNTGG	1 cut(s)	1621
PfoI	TCCNGGA	1 cut(s)	792
PkrI	GCNGC	8 cut(s)	372, 424, 534, 537, 540, 599, 1686, 2100
Ple19I	CGATCG	1 cut(s)	157
PleI	GAGTC	2 cut(s)	29, 664
PpsI	GAGTC	2 cut(s)	29, 664
Psp6I	CCWGG	5 cut(s)	18, 180, 414, 792, 1177
PspFI	CCCAGC	1 cut(s)	661
PspGI	CCWGG	5 cut(s)	18, 180, 414, 792, 1177
PspN4I	GGNNCC	2 cut(s)	145, 231
PspPI	GGNCC	3 cut(s)	22, 178, 1574
PstI	CTGCAG	1 cut(s)	537
PstNI	CAGNNNCTG	2 cut(s)	440, 1544
PsuI	RGATCY	1 cut(s)	1906
PvuI	CGATCG	1 cut(s)	157
PvuII	CAGCTG	1 cut(s)	538
RsaI	GTAC	4 cut(s)	117, 411, 930, 1377
RsaNI	GTAC	4 cut(s)	116, 410, 929, 1376
RseI	CAYNNNNRTG	2 cut(s)	645, 1713
SacII	CCGCGG	1 cut(s)	408
SapI	GCTCTTC	1 cut(s)	104
SatI	GCNGC	8 cut(s)	371, 423, 533, 536, 539, 598, 1685, 2099
Sau96I	GGNCC	3 cut(s)	22, 178, 1574
ScaI	AGTACT	1 cut(s)	1377
SchI	GAGTC	2 cut(s)	29, 664
ScrFI	CCNGG	6 cut(s)	20, 182, 247, 416, 794, 1179
SexAI	ACCWGGT	1 cut(s)	180
SfaNI	GCATC	6 cut(s)	637, 840, 862, 1150, 1842, 1882
SfcI	CTRYAG	3 cut(s)	441, 533, 1152
Sfr303I	CCGCGG	1 cut(s)	408
SgrBI	CCGCGG	1 cut(s)	408
SinI	GGWCC	3 cut(s)	22, 178, 1574
SmiMI	CAYNNNNRTG	2 cut(s)	645, 1713
SmlI	CTYRAG	6 cut(s)	452, 1083, 1133, 1409, 1643, 1691
SmoI	CTYRAG	6 cut(s)	452, 1083, 1133, 1409, 1643, 1691
SsiI	CCGC	9 cut(s)	25, 299, 370, 405, 407, 597, 903, 1108, 1685
SspMI	CTAG	2 cut(s)	426, 2127
StyD4I	CCNGG	6 cut(s)	18, 180, 245, 414, 792, 1177
StyI	CCWWGG	1 cut(s)	480
TaaI	ACNGT	6 cut(s)	49, 436, 891, 1032, 1310, 1335
TaiI	ACGT	3 cut(s)	113, 1009, 1629
TaqI	TCGA	6 cut(s)	75, 215, 239, 1003, 1926, 1989
TatI	WGTACW	2 cut(s)	928, 1375
TauI	GCSGC	3 cut(s)	373, 600, 1687
TfiI	GAWTC	7 cut(s)	173, 236, 779, 833, 1384, 1448, 1670
TscAI	CASTG	5 cut(s)	619, 894, 1035, 1315, 1605
TseFI	GTSAC	2 cut(s)	1294, 1627
TseI	GCWGC	5 cut(s)	422, 532, 535, 538, 2098
Tsp45I	GTSAC	2 cut(s)	1294, 1627
TspDTI	ATGAA	7 cut(s)	525, 591, 629, 792, 912, 1359, 1512
TspGWI	ACGGA	2 cut(s)	1625, 1712
TspRI	CASTG	5 cut(s)	619, 894, 1035, 1315, 1605
Van91I	CCANNNNNTGG	1 cut(s)	1621
Vha464I	CTTAAG	2 cut(s)	1409, 1691
VpaK11BI	GGWCC	3 cut(s)	22, 178, 1574
XagI	CCTNNNNNAGG	1 cut(s)	867
XapI	RAATTY	4 cut(s)	1302, 1395, 1740, 2089
XbaI	TCTAGA	1 cut(s)	2126
XceI	RCATGY	2 cut(s)	1295, 1786
XmiI	GTMKAC	1 cut(s)	54
XmnI	GAANNNNTTC	3 cut(s)	1350, 1708, 1740
XspI	CTAG	2 cut(s)	426, 2127
ZraI	GACGTC	1 cut(s)	1627
ZrmI	AGTACT	1 cut(s)	1377
Zsp2I	ATGCAT	1 cut(s)	855

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.