Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0009405

Physical Location & Seq

Genomic Coordinates Forward (+)

1748812 .. 1754371

Gene Structure 5560 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g03130.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2169 bp

ATGGTGACTCGGAGCTTCCAGGTCCGCCACAACGACTCCGATTTCGCCGTCGTCTACGACACCGACGACGGCCTCGAAGTCTTGAAATACCAGCTCTTCTCTCTCACCTCTGTACCTCCGGACGAGCAAAAGATCATCGGCGCCGATGACGATCGCGTCGTTTCAGACGACTCCGACCTGGCCGCGGTCTCGGAGAAGCTCCGGTTGGTTTCGATCAGTGACGATCAACCGGAACAGGCCGCCGGGAACGACGAGTTGCTGAAATCCGATGAGGAATTGGCTAGAATGTTGCAGGCGGAGGAGGAGGCGCTTATGTATCAGCAATTCGCTGTTCGTGAAGATAATGGAGCATTTGAGGGGAGAGTGCGGCCTTACATCAGTCAAGTTCTTATGTATGAGGATCCGATACGGCAGGAGGCTGCGAGGAAAACAGTCTCGGTAGAGCAGCTGGAGGAGAAGGCATTGGTCAGTTTGGCCAAGGAAGGGAACTTTACTCCATCAAAGAAGCAACAAGATCACGCGTTTCTGCTGCAGCTGCTATTCTGGTTCAAACAATCCTTCAGTTGGGTTAATCAACCTCCCTGTGATAGTTGCGGCCAGAAAACAGTCAGCCTTGGCATGGATGCTGCACTTCCTCCAGAACTTCTTCATGGAGGTTCACGAGTTGAAATTTATAGATGCACTTCTTGCCCAACAGTGACTCGTTTCCCACGCTACAATGACCCACTAAAGCTTGTGGAAACAAGAAGGGGTCGTTGTGGGGAATGGGCAAATTGCTTCACGCTTTATTGCCGAACTTTTGGGTTTGAATCCCGCCTGATCCTAGATTTCACGGATCATGTTTGGACAGAGTGCTTTTCAGAATCTTTAGGAAGATGGATGCATCTTGATCCTTGTGAAGGAGTGTATGACAAACCACTGTTATATGAAAGCGGGTGGAGTAAAAAATTAAATTATGTAATTGCCATTGGGAAGGACGGCGTATGTGATGTAACGAAACGGTACACAAGGAAGTGGCATGAGGTTCTTTCTCGACGTAACATTATTTCAGAGCCTGCACTGTCATCTGTGCTTGCTAATATAACAAAAGAATGTCGACGAGGCTATACTTCTCAAGTGCGTTCTGCACTTGAAGACCGCGATGAGAAGGAAAGACAAGAACTAGAGAGAGATTTACGTTCTACAGACGATGCCTCAACCTCATTACCTGGGAGACGAAGTGGGGACAAGGAATGGCGCAAGTCAAGAATGGAAGATGGCTCTGATGAGAGTTTATCCTTAACTGGTTCTTCTTGTCCACTTCGTCAATGTGTGGACGAGCACGTGACAAAAATTTACAATGGTTTTCTTCCAATTCTTGCACAGTTGGTAGATGAAGGTTTTCCAAAGTCAAGGGCAGTTGAAGTACTGGAGATTCTTAAAGGACTTCTCATAAATCTTAAGAAATCCCCTTTTAAAACAAGGAGGGTGTCAGTAGATTCAGTTCCAAATATCAACCAGTCTGTTGTTCGGCAGTTGCTGCCCTCTTTCACTGAGTTACTCAGCGCTCTTTCATTGAGTAGCAAGGCAGATACTGATGGGAGGGTTGAAATTTCATTGGCTGGACCTGCTGTTAAAACCTCTTTGGCACTGCCTGTTACATTCCATGCTTTGGACATCACAATCCGTAATCTTAAAAGTTGTGAAAACTTTGTTAAAGATTCTCTTTGCTTGCCACTTCTTAAGTTAAACAGAATACATTCTGGTGTAGTACGTGCAAGTGGAGAAGAAATTCCATTTGGAATTGCGACATCAGCTTTTGATGGGACACGCGTGTCCAAGTGGGAAGAACCAAATGGTGCCAAAGGTTGTTGGATCGTATATAAAGTATCGGATAACCAGATGCACGAACTTGTGGCATATGAGTTGATGTCAGCCAATGATGCACCTGAAAGAGATCCAATGGATTGGGTTGTCGAGAGAAGCAATGATGGGGGGTCAAGCTGGCACCTGATAGATAAACGAACTTCTCAACTATTCGACAGTCGTTTTCAGTGTAGAACATTTAAAGTTTCATCTGAAGGTTTCCTATCAAATGCTTTCAGGTTTAGATTTTTGAGGGTTCGCGATATTCAATCAAATTCGCGGCTGCAATTAGGTAGCATCGATCTCTATTCTAGAAGCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000224 GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006515 GO:0006516 GO:0006517 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009100 GO:0009636 GO:0009719 GO:0009725 GO:0009751 GO:0009987 GO:0010033 GO:0010035 GO:0010188 GO:0010193 GO:0014070 GO:0016787 GO:0016810 GO:0016811 GO:0019538 GO:0030163 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0050896 GO:0051603 GO:0071704 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Rad4 Transglut_core

Protein Analysis

723

Amino Acids

81.78

Weight (kDa)

5.58

Isoelectric Point (pI)

51.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transglut_core	PF01841	220 - 298	3.7e-15	Transglutaminase-like superfamily
Rad4	PF03835	280 - 388	1.4e-11	Rad4 transglutaminase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009405)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49570
fragaria_vesca	FvH4_6g03130 FvH4_6g03130
malus_domestica	MD12G1238800.v1.1
prunus_persica	Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1
pyrus_communis	pycom12g21950
rosa_chinensis	RchiOBHm_Chr3g0451381
rosa_laevigata	RLG00000025664
rosa_multiflora	Rmu_sc0002575.1_g000005 Rmu_sc0012229.1_g000001
rosa_roxburghii	Rroxscaffold_164G00436540 Rroxscaffold_6G00428800
rosa_rugosa	Rorug02G0633400
rosa_samantha	Rh3AG036400 Rh3BG037000 Rh3CG035600 Rh3DG036500
rosa_wichuraiana	Rw3G002680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	155, 1813
Acc36I	ACCTGC	1 cut(s)	1615
AccB1I	GGYRCC	3 cut(s)	140, 1838, 1986
AccB7I	CCANNNNNTGG	1 cut(s)	1651
AccI	GTMKAC	2 cut(s)	54, 1096
AccII	CGCG	7 cut(s)	156, 185, 521, 1140, 1812, 2106, 2125
AccIII	TCCGGA	1 cut(s)	118
AclWI	GGATC	7 cut(s)	395, 408, 814, 843, 884, 1862, 1931
AcoI	YGGCCR	3 cut(s)	180, 474, 595
AcsI	RAATTY	5 cut(s)	669, 1332, 1590, 1770, 2119
AcuI	CTGAAG	2 cut(s)	544, 2079
AcvI	CACGTG	1 cut(s)	1324
AcyI	GRCGYC	1 cut(s)	141
AfaI	GTAC	4 cut(s)	114, 1004, 1407, 1752
AfeI	AGCGCT	1 cut(s)	1546
AfiI	CCNNNNNNNGG	6 cut(s)	184, 483, 564, 619, 899, 1651
AflII	CTTAAG	2 cut(s)	1439, 1721
AflIII	ACRYGT	2 cut(s)	519, 1810
AgsI	TTSAA	8 cut(s)	85, 550, 668, 809, 1133, 1403, 1589, 2114
AjnI	CCWGG	3 cut(s)	18, 177, 1207
AjuI	GAANNNNNNNTTGG	4 cut(s)	188, 220, 1761, 1793
AluBI	AGCT	8 cut(s)	15, 94, 199, 448, 535, 733, 1796, 1983
AluI	AGCT	8 cut(s)	15, 94, 199, 448, 535, 733, 1796, 1983
Alw21I	GWGCWC	1 cut(s)	1323
Alw26I	GTCTC	3 cut(s)	193, 439, 1207
AlwI	GGATC	7 cut(s)	395, 408, 814, 843, 884, 1862, 1931
AlwNI	CAGNNNCTG	3 cut(s)	1055, 1519, 1574
Aor13HI	TCCGGA	1 cut(s)	118
Aor51HI	AGCGCT	1 cut(s)	1546
AoxI	GGCC	6 cut(s)	70, 180, 237, 368, 474, 595
ApeKI	GCWGC	8 cut(s)	419, 445, 529, 532, 535, 626, 1519, 2128
ApoI	RAATTY	5 cut(s)	669, 1332, 1590, 1770, 2119
ArsI	GACNNNNNNTTYG	2 cut(s)	26, 58
Asp700I	GAANNNNTTC	4 cut(s)	645, 1380, 1738, 1770
AspLEI	GCGC	4 cut(s)	143, 310, 1239, 1547
AspS9I	GGNCC	2 cut(s)	22, 1604
AsuC2I	CCSGG	1 cut(s)	244
AsuHPI	GGTGA	2 cut(s)	16, 97
AvaII	GGWCC	2 cut(s)	22, 1604
BaeI	ACNNNNGTAYC	2 cut(s)	96, 129
BalI	TGGCCA	1 cut(s)	476
BamHI	GGATCC	1 cut(s)	400
BanI	GGYRCC	3 cut(s)	140, 1838, 1986
BauI	CACGAG	1 cut(s)	660
BbrPI	CACGTG	1 cut(s)	1324
BbsI	GAAGAC	1 cut(s)	1140
Bbv12I	GWGCWC	1 cut(s)	1323
BbvI	GCAGC	8 cut(s)	406, 457, 516, 522, 544, 613, 1506, 2115
BccI	CCATC	6 cut(s)	505, 870, 1250, 1571, 1796, 1964
BceAI	ACGGC	4 cut(s)	32, 85, 425, 994
BciT130I	CCWGG	3 cut(s)	20, 179, 1209
BcnI	CCSGG	1 cut(s)	244
BcoDI	GTCTC	3 cut(s)	193, 439, 1207
BfaI	CTAG	4 cut(s)	282, 824, 1163, 2157
BfmI	CTRYAG	2 cut(s)	530, 1182
BfoI	RGCGCY	3 cut(s)	144, 311, 1548
BfrI	CTTAAG	2 cut(s)	1439, 1721
BfuAI	ACCTGC	1 cut(s)	1615
BmcAI	AGTACT	1 cut(s)	1407
Bme1390I	CCNGG	4 cut(s)	20, 179, 244, 1209
Bme18I	GGWCC	2 cut(s)	22, 1604
BmgT120I	GGNCC	2 cut(s)	22, 1604
BmiI	GGNNCC	4 cut(s)	142, 402, 1840, 1988
BmrFI	CCNGG	4 cut(s)	20, 179, 244, 1209
BmsI	GCATC	8 cut(s)	613, 668, 870, 892, 1180, 1872, 1912, 2151
BpiI	GAAGAC	1 cut(s)	1140
BpmI	CTGGAG	3 cut(s)	470, 621, 1430
BpuEI	CTTGAG	1 cut(s)	1098
BpuMI	CCSGG	1 cut(s)	244
Bsa29I	ATCGAT	1 cut(s)	2145
BsaAI	YACGTR	2 cut(s)	1324, 1754
BsaBI	GATNNNNATC	1 cut(s)	150
BsaHI	GRCGYC	1 cut(s)	141
BsaI	GGTCTC	1 cut(s)	193
BsaJI	CCNNGG	4 cut(s)	183, 477, 613, 1208
BsaWI	WCCGGW	3 cut(s)	118, 201, 229
BsaXI	ACNNNNNCTCC	4 cut(s)	20, 50, 1204, 1234
Bsc4I	CCNNNNNNNGG	6 cut(s)	184, 483, 564, 619, 899, 1651
Bse1I	ACTGG	3 cut(s)	1288, 1413, 1498
Bse3DI	GCAATG	1 cut(s)	1972
Bse8I	GATNNNNATC	1 cut(s)	150
BseAI	TCCGGA	1 cut(s)	118
BseBI	CCWGG	3 cut(s)	20, 179, 1209
BseCI	ATCGAT	1 cut(s)	2145
BseDI	CCNNGG	4 cut(s)	183, 477, 613, 1208
BseGI	GGATG	2 cut(s)	628, 885
BseJI	GATNNNNATC	1 cut(s)	150
BseLI	CCNNNNNNNGG	6 cut(s)	184, 483, 564, 619, 899, 1651
BseMI	GCAATG	1 cut(s)	1972
BseMII	CTCAG	2 cut(s)	1524, 1555
BseNI	ACTGG	3 cut(s)	1288, 1413, 1498
BseRI	GAGGAG	3 cut(s)	314, 317, 467
BseXI	GCAGC	8 cut(s)	406, 457, 516, 522, 544, 613, 1506, 2115
BsgI	GTGCAG	3 cut(s)	612, 1041, 1110
Bsh1236I	CGCG	7 cut(s)	156, 185, 521, 1140, 1812, 2106, 2125
Bsh1285I	CGRYCG	1 cut(s)	154
BshFI	GGCC	6 cut(s)	72, 182, 239, 370, 476, 597
BshNI	GGYRCC	3 cut(s)	140, 1838, 1986
BshVI	ATCGAT	1 cut(s)	2145
BsiEI	CGRYCG	1 cut(s)	154
BsiHKAI	GWGCWC	1 cut(s)	1323
BsiSI	CCGG	4 cut(s)	119, 202, 230, 243
BslFI	GGGAC	2 cut(s)	1238, 1819
BslI	CCNNNNNNNGG	6 cut(s)	184, 483, 564, 619, 899, 1651
BsmAI	GTCTC	3 cut(s)	193, 439, 1207
BsmBI	CGTCTC	1 cut(s)	1207
BsmFI	GGGAC	2 cut(s)	1238, 1819
BsnI	GGCC	6 cut(s)	72, 182, 239, 370, 476, 597
Bso31I	GGTCTC	1 cut(s)	193
Bsp1286I	GDGCHC	1 cut(s)	1323
Bsp13I	TCCGGA	1 cut(s)	118
Bsp68I	TCGCGA	1 cut(s)	2106
BspANI	GGCC	6 cut(s)	72, 182, 239, 370, 476, 597
BspCNI	CTCAG	2 cut(s)	1525, 1554
BspDI	ATCGAT	1 cut(s)	2145
BspEI	TCCGGA	1 cut(s)	118
BspFNI	CGCG	7 cut(s)	156, 185, 521, 1140, 1812, 2106, 2125
BspLI	GGNNCC	4 cut(s)	142, 402, 1840, 1988
BspMAI	CTGCAG	1 cut(s)	534
BspMI	ACCTGC	1 cut(s)	1615
BspPI	GGATC	7 cut(s)	395, 408, 814, 843, 884, 1862, 1931
BspQI	GCTCTTC	1 cut(s)	101
BspT107I	GGYRCC	3 cut(s)	140, 1838, 1986
BspTI	CTTAAG	2 cut(s)	1439, 1721
BspTNI	GGTCTC	1 cut(s)	193
BsrDI	GCAATG	1 cut(s)	1972
BsrI	ACTGG	3 cut(s)	1288, 1413, 1498
BssECI	CCNNGG	4 cut(s)	183, 477, 613, 1208
BssNI	GRCGYC	1 cut(s)	141
BssSI	CACGAG	1 cut(s)	660
BssT1I	CCWWGG	2 cut(s)	477, 613
Bst2BI	CACGAG	1 cut(s)	660
Bst2UI	CCWGG	3 cut(s)	20, 179, 1209
Bst4CI	ACNGT	8 cut(s)	433, 607, 697, 921, 1002, 1062, 1365, 2024
Bst6I	CTCTTC	1 cut(s)	101
BstACI	GRCGYC	1 cut(s)	141
BstAFI	CTTAAG	2 cut(s)	1439, 1721
BstAPI	GCANNNNNTGC	2 cut(s)	687, 1519
BstBAI	YACGTR	2 cut(s)	1324, 1754
BstC8I	GCNNGC	5 cut(s)	294, 1056, 1074, 1712, 1985
BstDEI	CTNAG	2 cut(s)	1533, 1541
BstDSI	CCRYGG	1 cut(s)	183
BstENI	CCTNNNNNAGG	1 cut(s)	897
BstF5I	GGATG	2 cut(s)	628, 885
BstFNI	CGCG	7 cut(s)	156, 185, 521, 1140, 1812, 2106, 2125
BstH2I	RGCGCY	3 cut(s)	144, 311, 1548
BstHHI	GCGC	4 cut(s)	143, 310, 1239, 1547
BstMAI	GTCTC	3 cut(s)	193, 439, 1207
BstMCI	CGRYCG	1 cut(s)	154
BstMWI	GCNNNNNNNGC	6 cut(s)	535, 687, 1519, 1607, 1793, 1922
BstNI	CCWGG	3 cut(s)	20, 179, 1209
BstSCI	CCNGG	4 cut(s)	18, 177, 242, 1207
BstSFI	CTRYAG	2 cut(s)	530, 1182
BstUI	CGCG	7 cut(s)	156, 185, 521, 1140, 1812, 2106, 2125
BstV1I	GCAGC	8 cut(s)	406, 457, 516, 522, 544, 613, 1506, 2115
BstV2I	GAAGAC	1 cut(s)	1140
BstX2I	RGATCY	2 cut(s)	400, 1936
BstXI	CCANNNNNNTGG	1 cut(s)	1947
BstYI	RGATCY	2 cut(s)	400, 1936
Bsu15I	ATCGAT	1 cut(s)	2145
BsuRI	GGCC	6 cut(s)	72, 182, 239, 370, 476, 597
BsuTUI	ATCGAT	1 cut(s)	2145
BtgI	CCRYGG	1 cut(s)	183
BtgZI	GCGATG	1 cut(s)	1155
BtsCI	GGATG	2 cut(s)	628, 885
BtsI	GCAGTG	1 cut(s)	1628
BtsIMutI	CAGTG	7 cut(s)	223, 702, 917, 1058, 1530, 1628, 2039
BtuMI	TCGCGA	1 cut(s)	2106
BveI	ACCTGC	1 cut(s)	1615
Cac8I	GCNNGC	5 cut(s)	294, 1056, 1074, 1712, 1985
CaiI	CAGNNNCTG	3 cut(s)	1055, 1519, 1574
CfoI	GCGC	4 cut(s)	143, 310, 1239, 1547
Cfr13I	GGNCC	2 cut(s)	22, 1604
Cfr42I	CCGCGG	1 cut(s)	186
ClaI	ATCGAT	1 cut(s)	2145
CseI	GACGC	1 cut(s)	145
Csp6I	GTAC	4 cut(s)	113, 1003, 1406, 1751
CviAII	CATG	5 cut(s)	619, 650, 839, 1019, 1646
CviQI	GTAC	4 cut(s)	113, 1003, 1406, 1751
DdeI	CTNAG	2 cut(s)	1533, 1541
DinI	GGCGCC	1 cut(s)	142
DraI	TTTAAA	2 cut(s)	1456, 2047
DrdI	GACNNNNNNGTC	2 cut(s)	155, 1813
DseDI	GACNNNNNNGTC	2 cut(s)	155, 1813
EaeI	YGGCCR	3 cut(s)	180, 474, 595
Eam1104I	CTCTTC	1 cut(s)	101
EarI	CTCTTC	1 cut(s)	101
EciI	GGCGGA	2 cut(s)	14, 311
Eco130I	CCWWGG	2 cut(s)	477, 613
Eco31I	GGTCTC	1 cut(s)	193
Eco47I	GGWCC	2 cut(s)	22, 1604
Eco47III	AGCGCT	1 cut(s)	1546
Eco57I	CTGAAG	2 cut(s)	544, 2079
Eco72I	CACGTG	1 cut(s)	1324
EcoNI	CCTNNNNNAGG	1 cut(s)	897
EcoRII	CCWGG	3 cut(s)	18, 177, 1207
EcoT14I	CCWWGG	2 cut(s)	477, 613
EcoT22I	ATGCAT	1 cut(s)	885
EgeI	GGCGCC	1 cut(s)	142
EheI	GGCGCC	1 cut(s)	142
ErhI	CCWWGG	2 cut(s)	477, 613
Esp3I	CGTCTC	1 cut(s)	1207
FaeI	CATG	5 cut(s)	622, 653, 842, 1022, 1649
FalI	AAGNNNNNCTT	2 cut(s)	1689, 1721
FaqI	GGGAC	2 cut(s)	1238, 1819
FatI	CATG	5 cut(s)	618, 649, 838, 1018, 1645
FauI	CCCGC	2 cut(s)	821, 926
FauNDI	CATATG	1 cut(s)	1900
FblI	GTMKAC	2 cut(s)	54, 1096
FokI	GGATG	2 cut(s)	635, 892
FspBI	CTAG	4 cut(s)	282, 824, 1163, 2157
GlaI	GCGC	4 cut(s)	142, 309, 1238, 1546
GsuI	CTGGAG	3 cut(s)	470, 621, 1430
HaeII	RGCGCY	3 cut(s)	144, 311, 1548
HaeIII	GGCC	6 cut(s)	72, 182, 239, 370, 476, 597
HapII	CCGG	4 cut(s)	119, 202, 230, 243
HgaI	GACGC	1 cut(s)	145
HhaI	GCGC	4 cut(s)	143, 310, 1239, 1547
Hin1I	GRCGYC	1 cut(s)	141
Hin1II	CATG	5 cut(s)	622, 653, 842, 1022, 1649
Hin6I	GCGC	4 cut(s)	141, 308, 1237, 1545
HinP1I	GCGC	4 cut(s)	141, 308, 1237, 1545
HincII	GTYRAC	1 cut(s)	1097
HindII	GTYRAC	1 cut(s)	1097
HindIII	AAGCTT	1 cut(s)	731
HinfI	GANTC	9 cut(s)	7, 35, 170, 700, 809, 863, 1414, 1478, 1700
HpaII	CCGG	4 cut(s)	119, 202, 230, 243
HphI	GGTGA	2 cut(s)	16, 97
Hpy166II	GTNNAC	6 cut(s)	55, 659, 1005, 1097, 1298, 1315
Hpy8I	GTNNAC	6 cut(s)	55, 659, 1005, 1097, 1298, 1315
Hpy99I	CGWCG	7 cut(s)	53, 68, 71, 161, 254, 1038, 1101
HpyAV	CCTTC	9 cut(s)	451, 476, 568, 741, 893, 967, 1141, 1370, 2054
HpyCH4III	ACNGT	8 cut(s)	433, 607, 697, 921, 1002, 1062, 1365, 2024
HpyCH4IV	ACGT	4 cut(s)	1036, 1177, 1323, 1753
HpyF10VI	GCNNNNNNNGC	6 cut(s)	535, 687, 1519, 1607, 1793, 1922
HpyF3I	CTNAG	2 cut(s)	1533, 1541
HpySE526I	ACGT	4 cut(s)	1036, 1177, 1323, 1753
Hsp92I	GRCGYC	1 cut(s)	141
Hsp92II	CATG	5 cut(s)	622, 653, 842, 1022, 1649
HspAI	GCGC	4 cut(s)	141, 308, 1237, 1545
KasI	GGCGCC	1 cut(s)	140
Kpn2I	TCCGGA	1 cut(s)	118
KspI	CCGCGG	1 cut(s)	186
LguI	GCTCTTC	1 cut(s)	101
LmnI	GCTCC	3 cut(s)	12, 204, 347
Lsp1109I	GCAGC	8 cut(s)	406, 457, 516, 522, 544, 613, 1506, 2115
LweI	GCATC	8 cut(s)	613, 668, 870, 892, 1180, 1872, 1912, 2151
MaeI	CTAG	4 cut(s)	282, 824, 1163, 2157
MaeII	ACGT	4 cut(s)	1036, 1177, 1323, 1753
MaeIII	GTNAC	8 cut(s)	4, 218, 697, 991, 1037, 1324, 1536, 1636
MflI	RGATCY	2 cut(s)	400, 1936
MhlI	GDGCHC	1 cut(s)	1323
MlsI	TGGCCA	1 cut(s)	476
MluI	ACGCGT	2 cut(s)	519, 1810
MluNI	TGGCCA	1 cut(s)	476
Mly113I	GGCGCC	1 cut(s)	141
MlyI	GAGTC	3 cut(s)	29, 164, 694
MmeI	TCCRAC	2 cut(s)	198, 1832
Mox20I	TGGCCA	1 cut(s)	476
Mph1103I	ATGCAT	1 cut(s)	885
MroI	TCCGGA	1 cut(s)	118
MroXI	GAANNNNTTC	4 cut(s)	645, 1380, 1738, 1770
MscI	TGGCCA	1 cut(s)	476
MslI	CAYNNNNRTG	1 cut(s)	1743
Msp20I	TGGCCA	1 cut(s)	476
MspA1I	CMGCKG	3 cut(s)	185, 448, 535
MspCI	CTTAAG	2 cut(s)	1439, 1721
MspI	CCGG	4 cut(s)	119, 202, 230, 243
MspR9I	CCNGG	4 cut(s)	20, 179, 244, 1209
MvaI	CCWGG	3 cut(s)	20, 179, 1209
MvnI	CGCG	7 cut(s)	156, 185, 521, 1140, 1812, 2106, 2125
MwoI	GCNNNNNNNGC	6 cut(s)	535, 687, 1519, 1607, 1793, 1922
NarI	GGCGCC	1 cut(s)	141
NciI	CCSGG	1 cut(s)	244
NdeI	CATATG	1 cut(s)	1900
NlaIII	CATG	5 cut(s)	622, 653, 842, 1022, 1649
NlaIV	GGNNCC	4 cut(s)	142, 402, 1840, 1988
NmuCI	GTSAC	4 cut(s)	4, 218, 697, 1324
NruI	TCGCGA	1 cut(s)	2106
NsiI	ATGCAT	1 cut(s)	885
PciSI	GCTCTTC	1 cut(s)	101
PcsI	WCGNNNNNNNCGW	4 cut(s)	63, 72, 156, 165
PdmI	GAANNNNTTC	4 cut(s)	645, 1380, 1738, 1770
PfeI	GAWTC	5 cut(s)	809, 863, 1414, 1478, 1700
PflMI	CCANNNNNTGG	1 cut(s)	1651
Ple19I	CGATCG	1 cut(s)	154
PleI	GAGTC	3 cut(s)	29, 164, 694
PluTI	GGCGCC	1 cut(s)	144
PmaCI	CACGTG	1 cut(s)	1324
PmlI	CACGTG	1 cut(s)	1324
PpsI	GAGTC	3 cut(s)	29, 164, 694
Ppu21I	YACGTR	2 cut(s)	1324, 1754
Psp6I	CCWGG	3 cut(s)	18, 177, 1207
PspCI	CACGTG	1 cut(s)	1324
PspGI	CCWGG	3 cut(s)	18, 177, 1207
PspN4I	GGNNCC	4 cut(s)	142, 402, 1840, 1988
PspPI	GGNCC	2 cut(s)	22, 1604
PsrI	GAACNNNNNNTAC	2 cut(s)	1467, 1499
PstI	CTGCAG	1 cut(s)	534
PstNI	CAGNNNCTG	3 cut(s)	1055, 1519, 1574
PsuI	RGATCY	2 cut(s)	400, 1936
PvuI	CGATCG	1 cut(s)	154
PvuII	CAGCTG	2 cut(s)	448, 535
RruI	TCGCGA	1 cut(s)	2106
RsaI	GTAC	4 cut(s)	114, 1004, 1407, 1752
RsaNI	GTAC	4 cut(s)	113, 1003, 1406, 1751
RseI	CAYNNNNRTG	1 cut(s)	1743
SacII	CCGCGG	1 cut(s)	186
SalI	GTCGAC	1 cut(s)	1095
SapI	GCTCTTC	1 cut(s)	101
Sau96I	GGNCC	2 cut(s)	22, 1604
ScaI	AGTACT	1 cut(s)	1407
SchI	GAGTC	3 cut(s)	29, 164, 694
ScrFI	CCNGG	4 cut(s)	20, 179, 244, 1209
SduI	GDGCHC	1 cut(s)	1323
SfaNI	GCATC	8 cut(s)	613, 668, 870, 892, 1180, 1872, 1912, 2151
SfcI	CTRYAG	2 cut(s)	530, 1182
SfoI	GGCGCC	1 cut(s)	142
Sfr303I	CCGCGG	1 cut(s)	186
SgrBI	CCGCGG	1 cut(s)	186
SinI	GGWCC	2 cut(s)	22, 1604
SmiMI	CAYNNNNRTG	1 cut(s)	1743
SmlI	CTYRAG	3 cut(s)	1113, 1439, 1721
SmoI	CTYRAG	3 cut(s)	1113, 1439, 1721
SspDI	GGCGCC	1 cut(s)	140
SspMI	CTAG	4 cut(s)	282, 824, 1163, 2157
StyD4I	CCNGG	4 cut(s)	18, 177, 242, 1207
StyI	CCWWGG	2 cut(s)	477, 613
TaaI	ACNGT	8 cut(s)	433, 607, 697, 921, 1002, 1062, 1365, 2024
TaiI	ACGT	4 cut(s)	1039, 1180, 1326, 1756
TaqI	TCGA	7 cut(s)	75, 212, 1033, 1096, 1956, 2019, 2145
TatI	WGTACW	1 cut(s)	1405
TauI	GCSGC	5 cut(s)	185, 242, 370, 597, 2128
TfiI	GAWTC	5 cut(s)	809, 863, 1414, 1478, 1700
TscAI	CASTG	7 cut(s)	223, 702, 924, 1065, 1537, 1635, 2039
TseFI	GTSAC	4 cut(s)	4, 218, 697, 1324
TseI	GCWGC	8 cut(s)	419, 445, 529, 532, 535, 626, 1519, 2128
Tsp45I	GTSAC	4 cut(s)	4, 218, 697, 1324
TspDTI	ATGAA	6 cut(s)	638, 942, 1389, 1542, 1584, 2043
TspGWI	ACGGA	2 cut(s)	848, 1655
TspRI	CASTG	7 cut(s)	223, 702, 924, 1065, 1537, 1635, 2039
Van91I	CCANNNNNTGG	1 cut(s)	1651
Vha464I	CTTAAG	2 cut(s)	1439, 1721
VpaK11BI	GGWCC	2 cut(s)	22, 1604
XagI	CCTNNNNNAGG	1 cut(s)	897
XapI	RAATTY	5 cut(s)	669, 1332, 1590, 1770, 2119
XbaI	TCTAGA	1 cut(s)	2156
XmiI	GTMKAC	2 cut(s)	54, 1096
XmnI	GAANNNNTTC	4 cut(s)	645, 1380, 1738, 1770
XspI	CTAG	4 cut(s)	282, 824, 1163, 2157
ZrmI	AGTACT	1 cut(s)	1407
Zsp2I	ATGCAT	1 cut(s)	885

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.