Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009405

Physical Location & Seq

Genomic Coordinates Reverse (-)

31177349 .. 31182517

Gene Structure 5169 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1238800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGGTGGCTCGGAGCTTCCAGGTTCACCACAAGGACTCCACTTTCTCCGTTGACTACGACACCGACGACGGTCTTGAAGTCTTCAAATTTCAACTGTTCTCCCTCACCTCCGTTCCCCCCGACGAACAAAAGTTAATTGGATTCGATGGAGATTCCGTTGTTTCGGACGATTCGGACCTCGTTTCGATTATCGAGAAGCTCCGATTGGTTTCGATCAGCGAAGAACAACAACCGCAAGAAGAATCAACGGTTCAGAACGACGAATTGCTGAAATCGGACGAGGAATTGGCTAGAATGTTGCAGGCAGAGGAGGAAGCACTTTTGTTTCAGCAGTACGCTGCCCATGAAGATGATGGAAAATTCGAGCAACAACTACGGCCTTATGTCAGTCAAGTTCTTATGTACGAGGACACGGTGCGCCAGGAGGCTGCTCGGAAAACAGTCCCTATAGAAGAGCTTGAGGAGAAGGCATTGGTCAGTTTGGCCAAGGAGGGGAACTTAACTCCGTCAAAAAATGAGCAAGATCATGCTTTCCTGCTGCAGCTACTTTTTTGGTTCAAACAGTCTTTCAGTTGGGTTAACTCGCCTGCCTGTGATAGTTGTGGCAATAACACCGTAAATATTGGCATGGCTACTGCAATTCCTTCAGAAATGCGATATGGAGCTTCTCGAGTTGAGACCTATAGATGCAATATTTGTTCTACGGTGACTCGTTTCCCACGCTACAATGATCCACTAAAGCTTGTGGAAACAAGAAGAGGGCGTTGTGGGGAATGGGCCAACTGCTTTACGCTTTATTGCCGAGCTTTTGGATATGAATCCCGTCTTATCTTGGATTTCACAGATCATGTTTGGACAGAGTGCTTCTCACAATCTTTGGGAAGATGGATGCATCTTGATCCTTGTGAAGCAGTGTATGATAAACCCCTGTTATATGAAAGCGGGTGGAACAAGAAATTGAATTACGTAATCGCCATCGCAAAAGATGGTGTTTGTGATGTAACCAAACGCTATACGAGGAAGTGGCTTGAGGTTCTTTCTCGACGTAACATCATTACAGAGCCTGCATTGTCAGCTGTGCTTGCTAATATAACAAAAGACTGTCGAAGAGGGTTTACTTCTCAAGTACTTTCTGTACTTGAAGAGCGTGATGAGAAGGAAAGGCAAGAACTCGAAAGAGGTTTGCATTCTACAGACAATGACTCAAGCTCATTACCTGGGAGACGAAGTGGGGACAAGGAATGGCGCAAGTCAAGATTAGAATGTGGTTCTGATGAGAGTTGCTCCTTGAGTGGTTCTTGTTGTCCAGTTCGTTCATGCTTTGACGAGCACGTGACCAAAATTCATAATGCATTTCTCCCAATTCTTTCAAAGCTTGTTGAGGAAGAATTTCCAAAGTCAAGGGCCGTTGAAGTACTTGAGATTCTAAAAGGCATTCTCATGGATCTTAAGAAATCACCTTTTAAAACAAGGAGGGCCACAATCGATTCAGTTTCTAATATCAACCAATCACTTGTTCATCAGTTGTTGCCCTCTTTCACTGAGTTACTTAATGCTCTTTCAATGAGTGTTATGGTAGATGGTGATGGGAAAGTTGACATTTCTCTGGCTGGAAGTGCTGTTAAAACTTCTTTGGCACTACCTGTTGCATTGGATGCTTTGGACGACACAATAAATAATCTTAACAATTGTGATAACTTTGTTGAAAAGTCTCTTTGCTTGCCTCTTCTGAAGCTAAACAGAATACATTCTGGTTCAGTCCTTGCAAGTGGCGAAGAAATTCCTTTTGGAATTGCCACTTCAGCGTTTGATGGGATACGCAAGTCTAAGTGGGAAGAACCAAATGGTGCACGAGGTTGCTGGATCATGTATAAAGTATCAGAGAACCAGATGCACGAACTTGTGGCGTATGAGTTAATGTCAGCCAACGATGTACCAGAAAGGGATCCCATGGATTGGGTTGTTGAAGGAAGCAATGATGAGGGATCAAGCTGGCATCTGCTGGATAAACAAACTTCTCAAGTATTTGATAGTCGTTTTCAGCGTAAAACATTTCAGATTGCTTCTCAAGGTTTCCTCGCAAATGCTTTCAGGTTTAGATTTTTGGCCGTTAAAGACGTCCAATCAAATTCACGGCTGCAATTAGGTAGCATTGACCTCTATTCAAGAAGCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000224 GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006508 GO:0006515 GO:0006516 GO:0006517 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009100 GO:0009636 GO:0009719 GO:0009725 GO:0009751 GO:0009987 GO:0010033 GO:0010035 GO:0010188 GO:0010193 GO:0014070 GO:0016787 GO:0016810 GO:0016811 GO:0019538 GO:0030163 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0050896 GO:0051603 GO:0071704 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Rad4 Transglut_core

Protein Analysis

726

Amino Acids

82.1

Weight (kDa)

5.09

Isoelectric Point (pI)

57.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transglut_core	PF01841	218 - 301	1.7e-16	Transglutaminase-like superfamily
Rad4	PF03835	283 - 391	4.5e-12	Rad4 transglutaminase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009405)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49570
fragaria_vesca	FvH4_6g03130 FvH4_6g03130
malus_domestica	MD12G1238800.v1.1
prunus_persica	Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1 Prupe.6G341700_v2.0.a1
pyrus_communis	pycom12g21950
rosa_chinensis	RchiOBHm_Chr3g0451381
rosa_laevigata	RLG00000025664
rosa_multiflora	Rmu_sc0002575.1_g000005 Rmu_sc0012229.1_g000001
rosa_roxburghii	Rroxscaffold_164G00436540 Rroxscaffold_6G00428800
rosa_rugosa	Rorug02G0633400
rosa_samantha	Rh3AG036400 Rh3BG037000 Rh3CG035600 Rh3DG036500
rosa_wichuraiana	Rw3G002680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2121
AccB7I	CCANNNNNTGG	1 cut(s)	1956
AciI	CCGC	2 cut(s)	233, 942
AclWI	GGATC	7 cut(s)	725, 893, 1452, 1871, 1940, 1953, 1993
AcoI	YGGCCR	2 cut(s)	483, 2106
AcsI	RAATTY	6 cut(s)	86, 359, 1341, 1388, 1779, 2128
AcuI	CTGAAG	3 cut(s)	630, 1751, 1785
AcvI	CACGTG	1 cut(s)	1333
AcyI	GRCGYC	1 cut(s)	2118
AfaI	GTAC	6 cut(s)	335, 404, 1128, 1137, 1416, 1935
AfiI	CCNNNNNNNGG	2 cut(s)	492, 1956
AflII	CTTAAG	1 cut(s)	1448
AjnI	CCWGG	3 cut(s)	18, 420, 1216
AjuI	GAANNNNNNNTTGG	6 cut(s)	188, 220, 1500, 1532, 1770, 1802
Alw21I	GWGCWC	2 cut(s)	1332, 1852
Alw26I	GTCTC	3 cut(s)	671, 1216, 1716
Alw44I	GTGCAC	1 cut(s)	1848
AlwI	GGATC	7 cut(s)	725, 893, 1452, 1871, 1940, 1953, 1993
AlwNI	CAGNNNCTG	1 cut(s)	1064
Ama87I	CYCGRG	1 cut(s)	669
AoxI	GGCC	6 cut(s)	377, 483, 777, 1404, 1476, 2106
ApaLI	GTGCAC	1 cut(s)	1848
ApeKI	GCWGC	5 cut(s)	338, 428, 538, 541, 2137
ApoI	RAATTY	6 cut(s)	86, 359, 1341, 1388, 1779, 2128
Asp700I	GAANNNNTTC	3 cut(s)	1389, 1747, 1779
AspLEI	GCGC	2 cut(s)	420, 1248
AspS9I	GGNCC	4 cut(s)	175, 777, 1404, 1476
AsuHPI	GGTGA	5 cut(s)	17, 97, 718, 1449, 1595
AvaI	CYCGRG	1 cut(s)	669
AvaII	GGWCC	1 cut(s)	175
BaeGI	GKGCMC	1 cut(s)	1852
BaeI	ACNNNNGTAYC	2 cut(s)	1808, 1841
BalI	TGGCCA	1 cut(s)	485
BamHI	GGATCC	1 cut(s)	1945
BarI	GAAGNNNNNNTAC	2 cut(s)	1099, 1131
BauI	CACGAG	1 cut(s)	1851
BbrPI	CACGTG	1 cut(s)	1333
BbsI	GAAGAC	1 cut(s)	73
Bbv12I	GWGCWC	2 cut(s)	1332, 1852
BbvI	GCAGC	5 cut(s)	325, 415, 525, 553, 2124
BccI	CCATC	8 cut(s)	140, 347, 879, 980, 983, 1574, 1580, 1805
BceAI	ACGGC	4 cut(s)	392, 1391, 2093, 2150
BciT130I	CCWGG	3 cut(s)	20, 422, 1218
BciVI	GTATCC	1 cut(s)	1809
BcoDI	GTCTC	3 cut(s)	671, 1216, 1716
BfaI	CTAG	1 cut(s)	291
BfmI	CTRYAG	4 cut(s)	447, 539, 682, 1191
BfrI	CTTAAG	1 cut(s)	1448
BfuI	GTATCC	1 cut(s)	1809
BisI	GCNGC	5 cut(s)	339, 429, 539, 542, 2138
BlsI	GCNGC	5 cut(s)	340, 430, 540, 543, 2139
BmcAI	AGTACT	2 cut(s)	1128, 1416
Bme1390I	CCNGG	3 cut(s)	20, 422, 1218
Bme18I	GGWCC	1 cut(s)	175
BmeT110I	CYCGRG	1 cut(s)	669
BmgT120I	GGNCC	4 cut(s)	175, 777, 1404, 1476
BmiI	GGNNCC	1 cut(s)	1947
BmrFI	CCNGG	3 cut(s)	20, 422, 1218
BmsI	GCATC	6 cut(s)	677, 879, 901, 1645, 1881, 2005
BoxI	GACNNNNGTC	1 cut(s)	69
BpiI	GAAGAC	1 cut(s)	73
BplI	GAGNNNNNCTC	4 cut(s)	851, 883, 1268, 1300
BpuEI	CTTGAG	8 cut(s)	479, 1049, 1107, 1189, 1309, 1439, 2004, 2052
Bsa29I	ATCGAT	1 cut(s)	1485
BsaAI	YACGTR	2 cut(s)	967, 1333
BsaBI	GATNNNNATC	1 cut(s)	817
BsaHI	GRCGYC	1 cut(s)	2118
BsaI	GGTCTC	1 cut(s)	671
BsaJI	CCNNGG	3 cut(s)	486, 1217, 1950
BsaXI	ACNNNNNCTCC	6 cut(s)	20, 50, 141, 171, 1213, 1243
Bsc4I	CCNNNNNNNGG	2 cut(s)	492, 1956
Bse1I	ACTGG	1 cut(s)	1307
Bse3DI	GCAATG	1 cut(s)	1981
Bse8I	GATNNNNATC	1 cut(s)	817
BseBI	CCWGG	3 cut(s)	20, 422, 1218
BseCI	ATCGAT	1 cut(s)	1485
BseDI	CCNNGG	3 cut(s)	486, 1217, 1950
BseGI	GGATG	2 cut(s)	894, 1660
BseJI	GATNNNNATC	1 cut(s)	817
BseLI	CCNNNNNNNGG	2 cut(s)	492, 1956
BseMI	GCAATG	1 cut(s)	1981
BseMII	CTCAG	1 cut(s)	1533
BseNI	ACTGG	1 cut(s)	1307
BseRI	GAGGAG	2 cut(s)	323, 476
BseSI	GKGCMC	1 cut(s)	1852
BseXI	GCAGC	5 cut(s)	325, 415, 525, 553, 2124
BshFI	GGCC	6 cut(s)	379, 485, 779, 1406, 1478, 2108
BshVI	ATCGAT	1 cut(s)	1485
BsiHKAI	GWGCWC	2 cut(s)	1332, 1852
BsiHKCI	CYCGRG	1 cut(s)	669
BslFI	GGGAC	2 cut(s)	428, 1247
BslI	CCNNNNNNNGG	2 cut(s)	492, 1956
BsmAI	GTCTC	3 cut(s)	671, 1216, 1716
BsmBI	CGTCTC	1 cut(s)	1216
BsmFI	GGGAC	2 cut(s)	428, 1247
BsmI	GAATGC	2 cut(s)	1186, 1434
BsnI	GGCC	6 cut(s)	379, 485, 779, 1406, 1478, 2108
Bso31I	GGTCTC	1 cut(s)	671
BsoBI	CYCGRG	1 cut(s)	669
Bsp1286I	GDGCHC	2 cut(s)	1332, 1852
Bsp143I	GATC	9 cut(s)	213, 523, 730, 844, 898, 1444, 1863, 1945, 1985
Bsp19I	CCATGG	1 cut(s)	1950
BspACI	CCGC	2 cut(s)	233, 942
BspANI	GGCC	6 cut(s)	379, 485, 779, 1406, 1478, 2108
BspCNI	CTCAG	1 cut(s)	1534
BspDI	ATCGAT	1 cut(s)	1485
BspLI	GGNNCC	1 cut(s)	1947
BspMAI	CTGCAG	1 cut(s)	543
BspPI	GGATC	7 cut(s)	725, 893, 1452, 1871, 1940, 1953, 1993
BspQI	GCTCTTC	2 cut(s)	447, 1137
BspTI	CTTAAG	1 cut(s)	1448
BspTNI	GGTCTC	1 cut(s)	671
BsrDI	GCAATG	1 cut(s)	1981
BsrI	ACTGG	1 cut(s)	1307
BssECI	CCNNGG	3 cut(s)	486, 1217, 1950
BssMI	GATC	9 cut(s)	213, 523, 730, 844, 898, 1444, 1863, 1945, 1985
BssNI	GRCGYC	1 cut(s)	2118
BssSI	CACGAG	1 cut(s)	1851
BssT1I	CCWWGG	2 cut(s)	486, 1950
Bst2BI	CACGAG	1 cut(s)	1851
Bst2UI	CCWGG	3 cut(s)	20, 422, 1218
Bst4CI	ACNGT	9 cut(s)	71, 96, 250, 415, 442, 564, 616, 706, 1103
Bst6I	CTCTTC	5 cut(s)	447, 751, 1102, 1137, 1731
BstACI	GRCGYC	1 cut(s)	2118
BstAFI	CTTAAG	1 cut(s)	1448
BstAPI	GCANNNNNTGC	1 cut(s)	1655
BstBAI	YACGTR	2 cut(s)	967, 1333
BstC8I	GCNNGC	6 cut(s)	303, 588, 1065, 1083, 1721, 1994
BstDEI	CTNAG	2 cut(s)	1542, 1827
BstDSI	CCRYGG	1 cut(s)	1950
BstF5I	GGATG	2 cut(s)	894, 1660
BstHHI	GCGC	2 cut(s)	420, 1248
BstKTI	GATC	9 cut(s)	216, 526, 733, 847, 901, 1447, 1866, 1948, 1988
BstMAI	GTCTC	3 cut(s)	671, 1216, 1716
BstMBI	GATC	9 cut(s)	213, 523, 730, 844, 898, 1444, 1863, 1945, 1985
BstMWI	GCNNNNNNNGC	5 cut(s)	1073, 1082, 1616, 1655, 1802
BstNI	CCWGG	3 cut(s)	20, 422, 1218
BstPAI	GACNNNNGTC	1 cut(s)	69
BstSCI	CCNGG	3 cut(s)	18, 420, 1216
BstSFI	CTRYAG	4 cut(s)	447, 539, 682, 1191
BstSLI	GKGCMC	1 cut(s)	1852
BstSNI	TACGTA	1 cut(s)	967
BstV1I	GCAGC	5 cut(s)	325, 415, 525, 553, 2124
BstV2I	GAAGAC	1 cut(s)	73
BstX2I	RGATCY	2 cut(s)	1444, 1945
BstYI	RGATCY	2 cut(s)	1444, 1945
Bsu15I	ATCGAT	1 cut(s)	1485
BsuI	GTATCC	1 cut(s)	1809
BsuRI	GGCC	6 cut(s)	379, 485, 779, 1406, 1478, 2108
BsuTUI	ATCGAT	1 cut(s)	1485
BtgI	CCRYGG	1 cut(s)	1950
BtgZI	GCGATG	1 cut(s)	961
BtsCI	GGATG	2 cut(s)	894, 1660
BtsI	GCAGTG	1 cut(s)	918
BtsIMutI	CAGTG	2 cut(s)	918, 1539
Cac8I	GCNNGC	6 cut(s)	303, 588, 1065, 1083, 1721, 1994
CaiI	CAGNNNCTG	1 cut(s)	1064
CfoI	GCGC	2 cut(s)	420, 1248
Cfr13I	GGNCC	4 cut(s)	175, 777, 1404, 1476
ClaI	ATCGAT	1 cut(s)	1485
Csp6I	GTAC	6 cut(s)	334, 403, 1127, 1136, 1415, 1934
CviAII	CATG	8 cut(s)	344, 527, 628, 848, 1317, 1441, 1867, 1951
CviQI	GTAC	6 cut(s)	334, 403, 1127, 1136, 1415, 1934
DdeI	CTNAG	2 cut(s)	1542, 1827
DpnI	GATC	9 cut(s)	215, 525, 732, 846, 900, 1446, 1865, 1947, 1987
DpnII	GATC	9 cut(s)	213, 523, 730, 844, 898, 1444, 1863, 1945, 1985
DraI	TTTAAA	1 cut(s)	1465
EaeI	YGGCCR	2 cut(s)	483, 2106
Eam1104I	CTCTTC	5 cut(s)	447, 751, 1102, 1137, 1731
EarI	CTCTTC	5 cut(s)	447, 751, 1102, 1137, 1731
Eco105I	TACGTA	1 cut(s)	967
Eco130I	CCWWGG	2 cut(s)	486, 1950
Eco31I	GGTCTC	1 cut(s)	671
Eco47I	GGWCC	1 cut(s)	175
Eco57I	CTGAAG	3 cut(s)	630, 1751, 1785
Eco72I	CACGTG	1 cut(s)	1333
Eco88I	CYCGRG	1 cut(s)	669
EcoRII	CCWGG	3 cut(s)	18, 420, 1216
EcoT14I	CCWWGG	2 cut(s)	486, 1950
EcoT22I	ATGCAT	2 cut(s)	894, 1354
ErhI	CCWWGG	2 cut(s)	486, 1950
Esp3I	CGTCTC	1 cut(s)	1216
FaeI	CATG	8 cut(s)	347, 530, 631, 851, 1320, 1444, 1870, 1954
FaqI	GGGAC	2 cut(s)	428, 1247
FatI	CATG	8 cut(s)	343, 526, 627, 847, 1316, 1440, 1866, 1950
FauI	CCCGC	1 cut(s)	935
Fnu4HI	GCNGC	5 cut(s)	339, 429, 539, 542, 2138
FokI	GGATG	2 cut(s)	901, 1667
Fsp4HI	GCNGC	5 cut(s)	339, 429, 539, 542, 2138
FspBI	CTAG	1 cut(s)	291
GlaI	GCGC	2 cut(s)	419, 1247
GluI	GCNGC	5 cut(s)	339, 429, 539, 542, 2138
HaeIII	GGCC	6 cut(s)	379, 485, 779, 1406, 1478, 2108
HhaI	GCGC	2 cut(s)	420, 1248
Hin1I	GRCGYC	1 cut(s)	2118
Hin1II	CATG	8 cut(s)	347, 530, 631, 851, 1320, 1444, 1870, 1954
Hin6I	GCGC	2 cut(s)	418, 1246
HinP1I	GCGC	2 cut(s)	418, 1246
HincII	GTYRAC	3 cut(s)	52, 580, 1597
HindII	GTYRAC	3 cut(s)	52, 580, 1597
HindIII	AAGCTT	2 cut(s)	740, 1373
HpaI	GTTAAC	1 cut(s)	580
HphI	GGTGA	5 cut(s)	17, 97, 718, 1449, 1595
Hpy166II	GTNNAC	6 cut(s)	25, 52, 580, 1116, 1597, 1850
Hpy188III	TCNNGA	7 cut(s)	74, 193, 669, 896, 1041, 1254, 2166
Hpy8I	GTNNAC	6 cut(s)	25, 52, 580, 1116, 1597, 1850
Hpy99I	CGWCG	5 cut(s)	68, 71, 125, 263, 1047
HpyAV	CCTTC	4 cut(s)	460, 654, 1150, 1961
HpyCH4III	ACNGT	9 cut(s)	71, 96, 250, 415, 442, 564, 616, 706, 1103
HpyCH4IV	ACGT	4 cut(s)	966, 1045, 1332, 2118
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1073, 1082, 1616, 1655, 1802
HpyF3I	CTNAG	2 cut(s)	1542, 1827
HpySE526I	ACGT	4 cut(s)	966, 1045, 1332, 2118
Hsp92I	GRCGYC	1 cut(s)	2118
Hsp92II	CATG	8 cut(s)	347, 530, 631, 851, 1320, 1444, 1870, 1954
HspAI	GCGC	2 cut(s)	418, 1246
KspAI	GTTAAC	1 cut(s)	580
Kzo9I	GATC	9 cut(s)	213, 523, 730, 844, 898, 1444, 1863, 1945, 1985
LguI	GCTCTTC	2 cut(s)	447, 1137
LmnI	GCTCC	4 cut(s)	12, 204, 662, 1289
Lsp1109I	GCAGC	5 cut(s)	325, 415, 525, 553, 2124
LweI	GCATC	6 cut(s)	677, 879, 901, 1645, 1881, 2005
MaeI	CTAG	1 cut(s)	291
MaeII	ACGT	4 cut(s)	966, 1045, 1332, 2118
MaeIII	GTNAC	5 cut(s)	706, 1000, 1046, 1333, 1545
MalI	GATC	9 cut(s)	215, 525, 732, 846, 900, 1446, 1865, 1947, 1987
MboI	GATC	9 cut(s)	213, 523, 730, 844, 898, 1444, 1863, 1945, 1985
MfeI	CAATTG	1 cut(s)	1687
MflI	RGATCY	2 cut(s)	1444, 1945
MhlI	GDGCHC	2 cut(s)	1332, 1852
MlsI	TGGCCA	1 cut(s)	485
MluNI	TGGCCA	1 cut(s)	485
MlyI	GAGTC	3 cut(s)	29, 703, 1196
Mox20I	TGGCCA	1 cut(s)	485
Mph1103I	ATGCAT	2 cut(s)	894, 1354
MroXI	GAANNNNTTC	3 cut(s)	1389, 1747, 1779
MscI	TGGCCA	1 cut(s)	485
MslI	CAYNNNNRTG	2 cut(s)	348, 1439
Msp20I	TGGCCA	1 cut(s)	485
MspA1I	CMGCKG	1 cut(s)	1076
MspCI	CTTAAG	1 cut(s)	1448
MspR9I	CCNGG	3 cut(s)	20, 422, 1218
MunI	CAATTG	1 cut(s)	1687
Mva1269I	GAATGC	2 cut(s)	1186, 1434
MvaI	CCWGG	3 cut(s)	20, 422, 1218
MwoI	GCNNNNNNNGC	5 cut(s)	1073, 1082, 1616, 1655, 1802
NcoI	CCATGG	1 cut(s)	1950
NdeII	GATC	9 cut(s)	213, 523, 730, 844, 898, 1444, 1863, 1945, 1985
NlaIII	CATG	8 cut(s)	347, 530, 631, 851, 1320, 1444, 1870, 1954
NlaIV	GGNNCC	1 cut(s)	1947
NmeAIII	GCCGAG	1 cut(s)	827
NmuCI	GTSAC	2 cut(s)	706, 1333
NsiI	ATGCAT	2 cut(s)	894, 1354
PaeR7I	CTCGAG	1 cut(s)	669
PciSI	GCTCTTC	2 cut(s)	447, 1137
PcsI	WCGNNNNNNNCGW	2 cut(s)	63, 2041
PctI	GAATGC	2 cut(s)	1186, 1434
PdmI	GAANNNNTTC	3 cut(s)	1389, 1747, 1779
PfeI	GAWTC	7 cut(s)	141, 152, 170, 242, 818, 1423, 1487
PflMI	CCANNNNNTGG	1 cut(s)	1956
PkrI	GCNGC	5 cut(s)	340, 430, 540, 543, 2139
PleI	GAGTC	3 cut(s)	29, 703, 1196
PmaCI	CACGTG	1 cut(s)	1333
PmlI	CACGTG	1 cut(s)	1333
PpsI	GAGTC	3 cut(s)	29, 703, 1196
Ppu21I	YACGTR	2 cut(s)	967, 1333
PshAI	GACNNNNGTC	1 cut(s)	69
Psp6I	CCWGG	3 cut(s)	18, 420, 1216
PspCI	CACGTG	1 cut(s)	1333
PspGI	CCWGG	3 cut(s)	18, 420, 1216
PspN4I	GGNNCC	1 cut(s)	1947
PspPI	GGNCC	4 cut(s)	175, 777, 1404, 1476
PstI	CTGCAG	1 cut(s)	543
PstNI	CAGNNNCTG	1 cut(s)	1064
PsuI	RGATCY	2 cut(s)	1444, 1945
PvuII	CAGCTG	1 cut(s)	1076
RsaI	GTAC	6 cut(s)	335, 404, 1128, 1137, 1416, 1935
RsaNI	GTAC	6 cut(s)	334, 403, 1127, 1136, 1415, 1934
RseI	CAYNNNNRTG	2 cut(s)	348, 1439
SapI	GCTCTTC	2 cut(s)	447, 1137
SatI	GCNGC	5 cut(s)	339, 429, 539, 542, 2138
Sau3AI	GATC	9 cut(s)	213, 523, 730, 844, 898, 1444, 1863, 1945, 1985
Sau96I	GGNCC	4 cut(s)	175, 777, 1404, 1476
ScaI	AGTACT	2 cut(s)	1128, 1416
SchI	GAGTC	3 cut(s)	29, 703, 1196
ScrFI	CCNGG	3 cut(s)	20, 422, 1218
SduI	GDGCHC	2 cut(s)	1332, 1852
SfaNI	GCATC	6 cut(s)	677, 879, 901, 1645, 1881, 2005
SfcI	CTRYAG	4 cut(s)	447, 539, 682, 1191
Sfr274I	CTCGAG	1 cut(s)	669
SinI	GGWCC	1 cut(s)	175
SlaI	CTCGAG	1 cut(s)	669
SmiMI	CAYNNNNRTG	2 cut(s)	348, 1439
SnaBI	TACGTA	1 cut(s)	967
SsiI	CCGC	2 cut(s)	233, 942
SspI	AATATT	2 cut(s)	622, 694
SspMI	CTAG	1 cut(s)	291
StyD4I	CCNGG	3 cut(s)	18, 420, 1216
StyI	CCWWGG	2 cut(s)	486, 1950
TaaI	ACNGT	9 cut(s)	71, 96, 250, 415, 442, 564, 616, 706, 1103
TaiI	ACGT	4 cut(s)	969, 1048, 1335, 2121
TatI	WGTACW	3 cut(s)	1126, 1135, 1414
TfiI	GAWTC	7 cut(s)	141, 152, 170, 242, 818, 1423, 1487
TscAI	CASTG	2 cut(s)	918, 1546
TseFI	GTSAC	2 cut(s)	706, 1333
TseI	GCWGC	5 cut(s)	338, 428, 538, 541, 2137
Tsp45I	GTSAC	2 cut(s)	706, 1333
TspDTI	ATGAA	6 cut(s)	360, 831, 951, 1305, 1334, 1508
TspGWI	ACGGA	4 cut(s)	37, 100, 145, 495
TspRI	CASTG	2 cut(s)	918, 1546
Van91I	CCANNNNNTGG	1 cut(s)	1956
Vha464I	CTTAAG	1 cut(s)	1448
VneI	GTGCAC	1 cut(s)	1848
VpaK11BI	GGWCC	1 cut(s)	175
XapI	RAATTY	6 cut(s)	86, 359, 1341, 1388, 1779, 2128
XcmI	CCANNNNNNNNNTGG	1 cut(s)	350
XhoI	CTCGAG	1 cut(s)	669
XmnI	GAANNNNTTC	3 cut(s)	1389, 1747, 1779
XspI	CTAG	1 cut(s)	291
ZraI	GACGTC	1 cut(s)	2119
ZrmI	AGTACT	2 cut(s)	1128, 1416
Zsp2I	ATGCAT	2 cut(s)	894, 1354

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.