AT5G49810

methionine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015103
Physical Location & Seq
Forward (+)
20239293 .. 20246398
7106 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G49810.1

Sequence Viewer

Length: 3216 bp
ATGGCGGACCTCTCCTCTGTCGATGAGTTCCTGAATCAGTGCAAACAATCCGGCGACGCAGCTTATGGCGCCTTGCGATCGGTCTTGGAGCGGCTCGAGGATCCGAATACTCGATCTAAGGCTCGGATCTTCTTGTCTGACATCTACAAACGCGTCGGATCTTCTGAGACGTCTCTCCAAACCTACCATTTTCATATCCAGGACATCTATCTCGATCAATACGAAGGCTTTCAGTCCAGGAAAAAGTTAACCATGATGGTCATTCCTAGTATTTTTATTCCAGAAGACTGGTCATTTACATTTTATGAAGGACTTAACAGACATCCTGACACCATCTTTAAGGATAAGACTGTTTCTGAACTTGGCTGTGGGAATGGATGGATATCAATAGCCATTGCTGCTAAGTGGTTGCCTTCAAAGGTATATGGGCTTGATATTAATCCTAGAGCTGTGAAAATTTCTTGGATAAATTTGTACCTAAACGCTCTTGATGATAATGGCGAACCAGTCTATGACGAAGAGAAGAAAACTTTATTGGACAGAGTGGAATTCTATGAATCTGATTTGCTTGGTTATTGTAGAGATAATAAAATTCAGTTAGAAAGAATTGTAGGATGCATACCTCAGATTCTTAATCCAAACCCAGAAGCTATGTCTAAGCTGATCACAGAAAATGCAAGTGAGGAATTTCTCCATTCGCTGAGTAACTATTGTGCCCTTCAGGGTTTTGTTGAAGATCAGTTTGGCTTAGGTTTGATTGCCAGAGCAGTTGAAGAAGGAATATCTGTCATCAAACCTGCAGGGATTATGATATTTAACATGGGTGGTCGTCCTGGGCAAGGTGTCTGTAGACGCTTGTTTGAGCGGCGAGGAGTCCGTGTTACACAGATGTGGCAGACTAAAATACTTCAGGCTGCAGATACTGATATCTCAGCATTAGTTGAAATTGAGAGGAGCAGCCCTCATCGTTTTGAGTTCTTTATGGGACTTTCTGGAGACCAACCAATTTGTGCTCGAACAGCATGGGCCTATGGGAAGGCTGGTGGCCGAATCTCCCATGCTTTATCGGTTTATAGTTGTCAGATTCGCCAACCAAATCTGGTTAAGATAATCTTTGACTTCTTGAAAAATGGATTCCAAGAAATCAGTAATTCACTGGATTTATCTTTTGAAGATGAAACTGTTGCTGATGAGAAGATTCCATTCCTAGCCTATCTTGCTAGTGTCTTGAAAAATAGCTCCTATTTCCCGTTTGAACCTCCAGCTGGCAGCAAAAGATTCTGCAGTCTAATTGCAGGCTTTATGAGGACATACCACCGTATACCAATTAATCAGGATAACATTGTCGTGTTTCCATCAAGGGCTGTGGCAATCGAGAGTGCATTTCGTTTATTCTCTCCTCGACTTGCAATTGTTGATGAGCATTTAACTCGACAACTTCCGAGGAGCTGGCTAACCTCTTTAGCCATTGAGGACACTAGCATGGATAAATCAGATGATCAAATCACAGTCATCGAATCCCCGCACCAATCTGATCTGATGATAGAACTCATTAAGAAACTAAAGCCACAGGTGGTGGTTACTGGAATGGCTCCATTTGAGGTCATTACCAGTTCATCGTTTTTGCACCTTTTGGAAGTGACAAAAGAAATTGGATGTCGACTTTTCTTGGATATATCTGATCACTTTGAGTTGTCTAGCCTTCCTGCATCCAATGGGGTACTAAAATATCTCGCTGAAAATCAACTACCTTCTCATGCAGCAATTATCTGCGGTCTGGTAAAGAATAAGGTCTATTCGGATTTAGAAGTAGCCTTTGTCATTACAGAAGTGGATGCCATTGCTAAAGCCCTGTCCAAAACTGTGGAAGTCTTAGAAGGTCATACTGCTATTATCAGTCAATACTACTATGGTTGCCTTTTCCATGAGCTTTTGGCTTTCCAGCTTGCTGATCGCCATGCCCCGGCTGAGAGGGAAAGTGAGAAAGCAAAGTCTGAAGAGATCATTGGATTTTCAAGCTCAGCAGTCTCTATTCTAAAAGATGCTGAGCTTTCAGTCACTGAGATTGACGAAACTTCTCTAATCCACATGGATGTTGATCAAAGTTTCTTGCAAATTCCACAATCTGTTAAGGCTGCAATCTTTGAAAGTTTTGTGAGGCAGAACATATCTGAAGCGGAAGTTGATATCAACCCAAGTATTAAGCAGTTTGTGTGGAGTAATTATGGGTTTCCAACCAAAAGCAGCACAGGCTTTGTATATGCTGATGGCTCACTAGCACTATTCAATAAACTGGTAATTTGTTGTGCTCAAGAAGGTGGAACGCTTTGTTTACCTGCTGGTACAAATGGAAATTATGTTGCTGCTGCCAAATTTTTAAAAGCTAATGTCGTGAATATCCCTACCGAGTCTAGTGATGGCTTTAAGCTGACAGAAAAGACTCTAACGAAAGCACTCGAGTCTGTGAAGAAGCCGTGGGTTTGTATATCTGGACCAACGGTTAGCCCTACAGGCTTGGTGTACAGCAATGAGGAGATGGATATACTGTTGTCTACTTGTGCTAAGTTTGGAGCAAAGGTCATCATCGATACTTCATTCTCGGGATTAGAATACAGTGCAACTAGCTGGGATTTGAAGAACGCTTTGTCGAAAATGGATTCTTCCTTATCAGTTTCGCTGCTTGGATGTCTCTCTTTGAATTTGCTCAGTGGAGCTATTAAACTCGGGTTTCTAGTTTTGGATCAGTCTCTCATCGATGCCTTCCATACCCTCCCAGGCCTGAGCAAACCGCACAGCACTGTGAAATATGCCGCTAAGAAAATGTTGGCTTTGAAGGAAGAGAAAGCAAGTGACTTTCTGGATGCCGTTTCTGAAACCATTAAAACCTTGGAAGGCAGATCCAGACGCTTAAAAGAGGTACTACAGAACTCTGGTTGGGAGGTTATCCAACCCTCAGCTGGAATCTCAATGGTGGCAAAGCCAAAAGCTTATCTCAACAAAAAAGTAAAGCTGAAAGCAGGAGATGGACAGGAAATCGTCGAGCTTACGGATTCAAATATGAGGGATGTGTTCCTCAGCCATACCGGTGTTTGCTTAAACAGCGGTTCCTGGACTGGAATCCCTGGTTACTGCCGGTTTTCATTTGCATTGGAAGATAGTGAGTTTGACAAGGCTATTGAATCGATAGCTCAGTTTAAAAGCGTCCTTGCTAACTGA

Protein Analysis

1071

Amino Acids

118.71

Weight (kDa)

5.43

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 735 - 1062 1.2e-23 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49810
fragaria_vesca FvH4_5g27550
malus_domestica MD08G1249200.v1.1 MD15G1443800.v1.1
prunus_persica Prupe.1G586400_v2.0.a1
pyrus_communis pycom08g21570
rosa_chinensis RchiOBHm_Chr7g0218921
rosa_laevigata RLG00000002317
rosa_multiflora Rmu_sc0003823.1_g000008
rosa_roxburghii Rroxscaffold_3G00240310
rosa_rugosa Rorug07G0179800
rosa_samantha Rh7AG323100 Rh7BG314100 Rh7CG339800 Rh7DG320200
rosa_wichuraiana Rw7G027380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 173
Acc36I ACCTGC 2 cut(s) 805, 2344
AccB1I GGYRCC 1 cut(s) 68
AccBSI CCGCTC 2 cut(s) 91, 865
AccI GTMKAC 4 cut(s) 850, 1321, 1660, 2552
AccII CGCG 1 cut(s) 153
AciI CCGC 9 cut(s) 5, 91, 865, 1523, 1773, 2177, 2789, 2811, 3102
AclWI GGATC 6 cut(s) 95, 108, 134, 166, 2748, 2892
AcoI YGGCCR 1 cut(s) 1045
AcsI RAATTY 8 cut(s) 456, 469, 548, 591, 686, 2115, 2372, 2698
AcuI CTGAAG 4 cut(s) 704, 893, 2016, 2193
AcyI GRCGYC 2 cut(s) 69, 170
AfaI GTAC 5 cut(s) 476, 1722, 2344, 2522, 2919
AfiI CCNNNNNNNGG 4 cut(s) 839, 1265, 1963, 2957
AflIII ACRYGT 1 cut(s) 151
AgeI ACCGGT 1 cut(s) 3083
AjnI CCWGG 6 cut(s) 198, 236, 832, 2773, 3107, 3121
AjuI GAANNNNNNNTTGG 6 cut(s) 518, 550, 726, 758, 1989, 2021
AleI CACNNNNGTG 1 cut(s) 889
Alw21I GWGCWC 2 cut(s) 1015, 2311
Alw26I GTCTC 6 cut(s) 161, 177, 990, 2032, 2693, 2751
AlwI GGATC 6 cut(s) 95, 108, 134, 166, 2748, 2892
AlwNI CAGNNNCTG 2 cut(s) 923, 2060
Ama87I CYCGRG 4 cut(s) 95, 2456, 2599, 2723
AoxI GGCC 3 cut(s) 1026, 1045, 2776
ApoI RAATTY 8 cut(s) 456, 469, 548, 591, 686, 2115, 2372, 2698
AseI ATTAAT 2 cut(s) 438, 1329
AsiGI ACCGGT 1 cut(s) 3083
Asp700I GAANNNNTTC 1 cut(s) 228
AspLEI GCGC 1 cut(s) 71
AspS9I GGNCC 3 cut(s) 7, 1026, 2492
AsuC2I CCSGG 1 cut(s) 1964
AvaI CYCGRG 4 cut(s) 95, 2456, 2599, 2723
AvaII GGWCC 2 cut(s) 7, 2492
BaeGI GKGCMC 1 cut(s) 718
BamHI GGATCC 1 cut(s) 100
BanI GGYRCC 1 cut(s) 68
BbsI GAAGAC 1 cut(s) 291
Bbv12I GWGCWC 2 cut(s) 1015, 2311
BbvCI CCTCAGC 2 cut(s) 2953, 3074
BccI CCATC 8 cut(s) 250, 341, 372, 1363, 2261, 2411, 2530, 3017
BceAI ACGGC 2 cut(s) 2458, 2849
BcgI CGANNNNNNTGC 2 cut(s) 1412, 1446
BciT130I CCWGG 6 cut(s) 200, 238, 834, 2775, 3109, 3123
BclI TGATCA 4 cut(s) 663, 1498, 1681, 2098
BcnI CCSGG 1 cut(s) 1964
BcoDI GTCTC 6 cut(s) 161, 177, 990, 2032, 2693, 2751
BfmI CTRYAG 6 cut(s) 798, 847, 915, 1282, 2508, 2921
BfoI RGCGCY 1 cut(s) 72
BfuAI ACCTGC 2 cut(s) 805, 2344
BglI GCCNNNNNGGC 1 cut(s) 2511
BlpI GCTNAGC 2 cut(s) 2020, 2046
Bme1390I CCNGG 7 cut(s) 200, 238, 834, 1964, 2775, 3109, 3123
Bme18I GGWCC 2 cut(s) 7, 2492
BmeT110I CYCGRG 4 cut(s) 95, 2456, 2599, 2723
BmgT120I GGNCC 3 cut(s) 7, 1026, 2492
BmiI GGNNCC 4 cut(s) 70, 102, 1593, 3106
BmrFI CCNGG 7 cut(s) 200, 238, 834, 1964, 2775, 3109, 3123
BmsI GCATC 6 cut(s) 605, 1718, 1825, 2032, 2746, 2851
BpiI GAAGAC 1 cut(s) 291
BplI GAGNNNNNCTC 2 cut(s) 946, 978
BpmI CTGGAG 2 cut(s) 1014, 1245
Bpu10I CCTNAGC 4 cut(s) 748, 2780, 2953, 3074
Bpu1102I GCTNAGC 2 cut(s) 2020, 2046
BpuEI CTTGAG 1 cut(s) 2295
BpuMI CCSGG 1 cut(s) 1964
Bsa29I ATCGAT 3 cut(s) 2586, 2754, 3182
BsaBI GATNNNNATC 3 cut(s) 382, 438, 2097
BsaHI GRCGYC 2 cut(s) 69, 170
BsaI GGTCTC 1 cut(s) 990
BsaJI CCNNGG 7 cut(s) 833, 1442, 1962, 2474, 2773, 2886, 3121
BsaWI WCCGGW 1 cut(s) 3083
BsaXI ACNNNNNCTCC 4 cut(s) 864, 894, 2562, 2592
Bsc4I CCNNNNNNNGG 4 cut(s) 839, 1265, 1963, 2957
Bse118I RCCGGY 2 cut(s) 3083, 3132
Bse1I ACTGG 7 cut(s) 293, 506, 1161, 1588, 1611, 2298, 3118
Bse3DI GCAATG 3 cut(s) 393, 1839, 2533
Bse8I GATNNNNATC 3 cut(s) 382, 438, 2097
BseBI CCWGG 6 cut(s) 200, 238, 834, 2775, 3109, 3123
BseCI ATCGAT 3 cut(s) 2586, 2754, 3182
BseDI CCNNGG 7 cut(s) 833, 1442, 1962, 2474, 2773, 2886, 3121
BseJI GATNNNNATC 3 cut(s) 382, 438, 2097
BseLI CCNNNNNNNGG 4 cut(s) 839, 1265, 1963, 2957
BseMI GCAATG 3 cut(s) 393, 1839, 2533
BseNI ACTGG 7 cut(s) 293, 506, 1161, 1588, 1611, 2298, 3118
BseRI GAGGAG 6 cut(s) 4, 885, 967, 1389, 1459, 2546
BseSI GKGCMC 1 cut(s) 718
BseYI CCCAGC 1 cut(s) 2625
Bsh1236I CGCG 1 cut(s) 153
Bsh1285I CGRYCG 1 cut(s) 80
BshFI GGCC 3 cut(s) 1028, 1047, 2778
BshNI GGYRCC 1 cut(s) 68
BshTI ACCGGT 1 cut(s) 3083
BshVI ATCGAT 3 cut(s) 2586, 2754, 3182
BsiEI CGRYCG 1 cut(s) 80
BsiHKAI GWGCWC 2 cut(s) 1015, 2311
BsiHKCI CYCGRG 4 cut(s) 95, 2456, 2599, 2723
BsiSI CCGG 4 cut(s) 51, 1964, 3084, 3133
BslFI GGGAC 1 cut(s) 999
BslI CCNNNNNNNGG 4 cut(s) 839, 1265, 1963, 2957
BsmAI GTCTC 6 cut(s) 161, 177, 990, 2032, 2693, 2751
BsmBI CGTCTC 2 cut(s) 161, 177
BsmFI GGGAC 1 cut(s) 999
BsnI GGCC 3 cut(s) 1028, 1047, 2778
Bso31I GGTCTC 1 cut(s) 990
BsoBI CYCGRG 4 cut(s) 95, 2456, 2599, 2723
Bsp1286I GDGCHC 3 cut(s) 718, 1015, 2311
Bsp1407I TGTACA 1 cut(s) 2520
Bsp1720I GCTNAGC 2 cut(s) 2020, 2046
BspACI CCGC 9 cut(s) 5, 91, 865, 1523, 1773, 2177, 2789, 2811, 3102
BspANI GGCC 3 cut(s) 1028, 1047, 2778
BspDI ATCGAT 3 cut(s) 2586, 2754, 3182
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 4 cut(s) 70, 102, 1593, 3106
BspMAI CTGCAG 3 cut(s) 802, 919, 1286
BspMI ACCTGC 2 cut(s) 805, 2344
BspPI GGATC 6 cut(s) 95, 108, 134, 166, 2748, 2892
BspT107I GGYRCC 1 cut(s) 68
BspTNI GGTCTC 1 cut(s) 990
BsrBI CCGCTC 2 cut(s) 91, 865
BsrDI GCAATG 3 cut(s) 393, 1839, 2533
BsrFI RCCGGY 2 cut(s) 3083, 3132
BsrGI TGTACA 1 cut(s) 2520
BsrI ACTGG 7 cut(s) 293, 506, 1161, 1588, 1611, 2298, 3118
BssAI RCCGGY 2 cut(s) 3083, 3132
BssECI CCNNGG 7 cut(s) 833, 1442, 1962, 2474, 2773, 2886, 3121
BssNAI GTATAC 1 cut(s) 1322
BssNI GRCGYC 2 cut(s) 69, 170
BssT1I CCWWGG 1 cut(s) 2886
Bst1107I GTATAC 1 cut(s) 1322
Bst2UI CCWGG 6 cut(s) 200, 238, 834, 2775, 3109, 3123
Bst4CI ACNGT 9 cut(s) 352, 1183, 1319, 1510, 1864, 2500, 2547, 2615, 2800
Bst6I CTCTTC 3 cut(s) 513, 1992, 2832
BstACI GRCGYC 2 cut(s) 69, 170
BstAUI TGTACA 1 cut(s) 2520
BstC8I GCNNGC 4 cut(s) 1267, 1297, 1451, 1947
BstDSI CCRYGG 1 cut(s) 2474
BstENI CCTNNNNNAGG 1 cut(s) 837
BstFNI CGCG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 1 cut(s) 71
BstMAI GTCTC 6 cut(s) 161, 177, 990, 2032, 2693, 2751
BstMCI CGRYCG 1 cut(s) 80
BstMWI GCNNNNNNNGC 7 cut(s) 68, 398, 1019, 1217, 2250, 2511, 3099
BstNI CCWGG 6 cut(s) 200, 238, 834, 2775, 3109, 3123
BstSCI CCNGG 7 cut(s) 198, 236, 832, 1962, 2773, 3107, 3121
BstSFI CTRYAG 6 cut(s) 798, 847, 915, 1282, 2508, 2921
BstSLI GKGCMC 1 cut(s) 718
BstUI CGCG 1 cut(s) 153
BstV2I GAAGAC 1 cut(s) 291
BstX2I RGATCY 4 cut(s) 100, 126, 158, 2897
BstXI CCANNNNNNTGG 2 cut(s) 288, 1864
BstYI RGATCY 4 cut(s) 100, 126, 158, 2897
BstZ17I GTATAC 1 cut(s) 1322
Bsu15I ATCGAT 3 cut(s) 2586, 2754, 3182
BsuRI GGCC 3 cut(s) 1028, 1047, 2778
BsuTUI ATCGAT 3 cut(s) 2586, 2754, 3182
BtgI CCRYGG 1 cut(s) 2474
BtsIMutI CAGTG 6 cut(s) 44, 1154, 2058, 2620, 2713, 2796
BveI ACCTGC 2 cut(s) 805, 2344
Cac8I GCNNGC 4 cut(s) 1267, 1297, 1451, 1947
CaiI CAGNNNCTG 2 cut(s) 923, 2060
CfoI GCGC 1 cut(s) 71
Cfr10I RCCGGY 2 cut(s) 3083, 3132
Cfr13I GGNCC 3 cut(s) 7, 1026, 2492
ClaI ATCGAT 3 cut(s) 2586, 2754, 3182
CseI GACGC 5 cut(s) 65, 142, 861, 2913, 3190
Csp6I GTAC 5 cut(s) 475, 1721, 2343, 2521, 2918
CspAI ACCGGT 1 cut(s) 3083
CspCI CAANNNNNGTGG 2 cut(s) 1347, 1382
CviAII CATG 9 cut(s) 253, 820, 1023, 1058, 1483, 1757, 1925, 1958, 2089
CviQI GTAC 5 cut(s) 475, 1721, 2343, 2521, 2918
DinI GGCGCC 1 cut(s) 70
DraI TTTAAA 2 cut(s) 2379, 3196
EaeI YGGCCR 1 cut(s) 1045
Eam1104I CTCTTC 3 cut(s) 513, 1992, 2832
EarI CTCTTC 3 cut(s) 513, 1992, 2832
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 2886
Eco147I AGGCCT 1 cut(s) 2778
Eco31I GGTCTC 1 cut(s) 990
Eco32I GATATC 3 cut(s) 384, 928, 2188
Eco47I GGWCC 2 cut(s) 7, 2492
Eco57I CTGAAG 4 cut(s) 704, 893, 2016, 2193
Eco88I CYCGRG 4 cut(s) 95, 2456, 2599, 2723
EcoNI CCTNNNNNAGG 1 cut(s) 837
EcoRI GAATTC 1 cut(s) 548
EcoRII CCWGG 6 cut(s) 198, 236, 832, 2773, 3107, 3121
EcoRV GATATC 3 cut(s) 384, 928, 2188
EcoT14I CCWWGG 1 cut(s) 2886
EcoT22I ATGCAT 1 cut(s) 620
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 2886
Esp3I CGTCTC 2 cut(s) 161, 177
FaeI CATG 9 cut(s) 256, 823, 1026, 1061, 1486, 1760, 1928, 1961, 2092
FalI AAGNNNNNCTT 2 cut(s) 1097, 1129
FaqI GGGAC 1 cut(s) 999
FatI CATG 9 cut(s) 252, 819, 1022, 1057, 1482, 1756, 1924, 1957, 2088
FauI CCCGC 1 cut(s) 1530
FbaI TGATCA 4 cut(s) 663, 1498, 1681, 2098
FblI GTMKAC 4 cut(s) 850, 1321, 1660, 2552
GlaI GCGC 1 cut(s) 70
GsaI CCCAGC 1 cut(s) 2629
GsuI CTGGAG 2 cut(s) 1014, 1245
HaeII RGCGCY 1 cut(s) 72
HaeIII GGCC 3 cut(s) 1028, 1047, 2778
HapII CCGG 4 cut(s) 51, 1964, 3084, 3133
HgaI GACGC 5 cut(s) 65, 142, 861, 2913, 3190
HhaI GCGC 1 cut(s) 71
Hin1I GRCGYC 2 cut(s) 69, 170
Hin1II CATG 9 cut(s) 256, 823, 1026, 1061, 1486, 1760, 1928, 1961, 2092
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HincII GTYRAC 2 cut(s) 249, 1661
HindII GTYRAC 2 cut(s) 249, 1661
HindIII AAGCTT 1 cut(s) 2985
HpaI GTTAAC 1 cut(s) 249
HpaII CCGG 4 cut(s) 51, 1964, 3084, 3133
Hpy166II GTNNAC 7 cut(s) 249, 851, 1322, 1661, 2333, 2521, 2553
Hpy8I GTNNAC 7 cut(s) 249, 851, 1322, 1661, 2333, 2521, 2553
Hpy99I CGWCG 3 cut(s) 59, 158, 3041
HpyCH4III ACNGT 9 cut(s) 352, 1183, 1319, 1510, 1864, 2500, 2547, 2615, 2800
HpyCH4IV ACGT 1 cut(s) 170
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 398, 1019, 1217, 2250, 2511, 3099
HpySE526I ACGT 1 cut(s) 170
Hsp92I GRCGYC 2 cut(s) 69, 170
Hsp92II CATG 9 cut(s) 256, 823, 1026, 1061, 1486, 1760, 1928, 1961, 2092
HspAI GCGC 1 cut(s) 69
KasI GGCGCC 1 cut(s) 68
Ksp22I TGATCA 4 cut(s) 663, 1498, 1681, 2098
KspAI GTTAAC 1 cut(s) 249
LmnI GCTCC 7 cut(s) 88, 954, 1244, 1446, 1597, 2570, 2711
LweI GCATC 6 cut(s) 605, 1718, 1825, 2032, 2746, 2851
MaeII ACGT 1 cut(s) 170
MaeIII GTNAC 7 cut(s) 704, 880, 1579, 1639, 2056, 2849, 3125
MbiI CCGCTC 2 cut(s) 91, 865
MfeI CAATTG 1 cut(s) 1410
MflI RGATCY 4 cut(s) 100, 126, 158, 2897
MhlI GDGCHC 3 cut(s) 718, 1015, 2311
MluI ACGCGT 1 cut(s) 151
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 4 cut(s) 882, 2417, 2434, 2468
MmeI TCCRAC 3 cut(s) 136, 2258, 2971
Mph1103I ATGCAT 1 cut(s) 620
MroXI GAANNNNTTC 1 cut(s) 228
MslI CAYNNNNRTG 5 cut(s) 889, 1346, 1481, 2091, 3084
MspA1I CMGCKG 3 cut(s) 1265, 2957, 3102
MspI CCGG 4 cut(s) 51, 1964, 3084, 3133
MspR9I CCNGG 7 cut(s) 200, 238, 834, 1964, 2775, 3109, 3123
MunI CAATTG 1 cut(s) 1410
MvaI CCWGG 6 cut(s) 200, 238, 834, 2775, 3109, 3123
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 7 cut(s) 68, 398, 1019, 1217, 2250, 2511, 3099
NarI GGCGCC 1 cut(s) 69
NciI CCSGG 1 cut(s) 1964
NlaIII CATG 9 cut(s) 256, 823, 1026, 1061, 1486, 1760, 1928, 1961, 2092
NlaIV GGNNCC 4 cut(s) 70, 102, 1593, 3106
NmuCI GTSAC 3 cut(s) 1639, 2056, 2849
NsiI ATGCAT 1 cut(s) 620
OliI CACNNNNGTG 1 cut(s) 889
PaeR7I CTCGAG 2 cut(s) 95, 2456
PceI AGGCCT 1 cut(s) 2778
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PdmI GAANNNNTTC 1 cut(s) 228
PfoI TCCNGGA 3 cut(s) 198, 236, 3107
PinAI ACCGGT 1 cut(s) 3083
Ple19I CGATCG 1 cut(s) 80
PleI GAGTC 4 cut(s) 881, 2416, 2434, 2467
PluTI GGCGCC 1 cut(s) 72
PpsI GAGTC 4 cut(s) 881, 2416, 2434, 2467
PshBI ATTAAT 2 cut(s) 438, 1329
Psp6I CCWGG 6 cut(s) 198, 236, 832, 2773, 3107, 3121
PspFI CCCAGC 1 cut(s) 2625
PspGI CCWGG 6 cut(s) 198, 236, 832, 2773, 3107, 3121
PspN4I GGNNCC 4 cut(s) 70, 102, 1593, 3106
PspPI GGNCC 3 cut(s) 7, 1026, 2492
PspXI VCTCGAGB 2 cut(s) 95, 2456
PstI CTGCAG 3 cut(s) 802, 919, 1286
PstNI CAGNNNCTG 2 cut(s) 923, 2060
PsuI RGATCY 4 cut(s) 100, 126, 158, 2897
PvuI CGATCG 1 cut(s) 80
PvuII CAGCTG 2 cut(s) 1265, 2957
RsaI GTAC 5 cut(s) 476, 1722, 2344, 2522, 2919
RsaNI GTAC 5 cut(s) 475, 1721, 2343, 2521, 2918
RseI CAYNNNNRTG 5 cut(s) 889, 1346, 1481, 2091, 3084
SalI GTCGAC 1 cut(s) 1659
Sau96I GGNCC 3 cut(s) 7, 1026, 2492
SbfI CCTGCAGG 1 cut(s) 802
SchI GAGTC 4 cut(s) 882, 2417, 2434, 2468
ScrFI CCNGG 7 cut(s) 200, 238, 834, 1964, 2775, 3109, 3123
SdaI CCTGCAGG 1 cut(s) 802
SduI GDGCHC 3 cut(s) 718, 1015, 2311
SfaNI GCATC 6 cut(s) 605, 1718, 1825, 2032, 2746, 2851
SfcI CTRYAG 6 cut(s) 798, 847, 915, 1282, 2508, 2921
SfoI GGCGCC 1 cut(s) 70
Sfr274I CTCGAG 2 cut(s) 95, 2456
SinI GGWCC 2 cut(s) 7, 2492
SlaI CTCGAG 2 cut(s) 95, 2456
SmiMI CAYNNNNRTG 5 cut(s) 889, 1346, 1481, 2091, 3084
SmlI CTYRAG 3 cut(s) 95, 2310, 2456
SmoI CTYRAG 3 cut(s) 95, 2310, 2456
Sse8387I CCTGCAGG 1 cut(s) 802
SseBI AGGCCT 1 cut(s) 2778
SsiI CCGC 9 cut(s) 5, 91, 865, 1523, 1773, 2177, 2789, 2811, 3102
SspDI GGCGCC 1 cut(s) 68
StuI AGGCCT 1 cut(s) 2778
StyD4I CCNGG 7 cut(s) 198, 236, 832, 1962, 2773, 3107, 3121
StyI CCWWGG 1 cut(s) 2886
TaaI ACNGT 9 cut(s) 352, 1183, 1319, 1510, 1864, 2500, 2547, 2615, 2800
TaiI ACGT 1 cut(s) 173
TaqII GACCGA 1 cut(s) 70
TatI WGTACW 1 cut(s) 2520
TauI GCSGC 3 cut(s) 94, 868, 2813
TscAI CASTG 6 cut(s) 44, 1161, 2065, 2620, 2713, 2803
TseFI GTSAC 3 cut(s) 1639, 2056, 2849
Tsp45I GTSAC 3 cut(s) 1639, 2056, 2849
TspDTI ATGAA 7 cut(s) 182, 321, 570, 1191, 1605, 2583, 3129
TspGWI ACGGA 2 cut(s) 866, 3062
TspRI CASTG 6 cut(s) 44, 1161, 2065, 2620, 2713, 2803
VpaK11BI GGWCC 2 cut(s) 7, 2492
VspI ATTAAT 2 cut(s) 438, 1329
XagI CCTNNNNNAGG 1 cut(s) 837
XapI RAATTY 8 cut(s) 456, 469, 548, 591, 686, 2115, 2372, 2698
XcmI CCANNNNNNNNNTGG 2 cut(s) 2884, 2954
XhoI CTCGAG 2 cut(s) 95, 2456
XmiI GTMKAC 4 cut(s) 850, 1321, 1660, 2552
XmnI GAANNNNTTC 1 cut(s) 228
ZraI GACGTC 1 cut(s) 171
Zsp2I ATGCAT 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.