pycom08g21570

Methionine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0015103
Physical Location & Seq
Reverse (-)
21028594 .. 21032024
3431 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g21570.6

Sequence Viewer

Length: 1362 bp
ATGGCGAAGAACGAGGCGCCGTTGGGATCGGTGGACGACTTCTTGAAGCAATGTCAGCAGTCGGGCGATGCCGCGTACGCTGCCTTAAGATCAGTGCTGGAGCGTCTCGAGGATCCGAAAACCAGAACTCAAGCTCGGATCTTCCTCACGGACCTCCAGAACCGCTTCCCCTCCAAGGAGGCCTGCGATCAATGCTTTCGCACATACCATTTTCAGATCGAAGATATCTTCTTCGATCAGTATGAAGGTTACCAGGGTAGGAAGAAATTGACGATGATGGTTATCCCCAGTATCTTTGTTCCAGAAGACTGGTCTTTTACTTTTTTTGAAGGACTAAATAGACATTCCGATTCTATCTTCAAGGACAAGTCAGTTGCAGAGCTTGGTTGTGGAAATGGTTGGATATCCATTGCCATTGCTGAGAAGTGGTTGCCATTGAAGGTGTATGGCCTTGAAATCAATCCTAGAGCAGTAAAGATGTCATGGATAAACTTGTACTTAAATGCGTTGGACGAAAGAGGTCAACCCATTTATGATGTGGAGAAGAAAACTTTGTTGGACAGGGTAGAGTTTCATGAATCAGATCTGCTATCTTATTGTAGAGATAATGACATTCAACTAGAACGAATTGTTGGATGCATACCTCAGATTCTTAACCCAAATCCAGATGCTATGTCTAAAATGATTACAGAGAACGCAAGTGAGGAATTTCTACATTCATTGAGCAATTATTGTGCACTTCAGGGTTTTCTTGAGGATCAGTTTGGCCTAGGTCTAATTGCTCGGGCAGTGGAGGAAGGAATAGCTGTCATTAAACCTATGGGAATAATGATCTTCAACATGGGGGGTCGTCCAGGACAAGCTGTTTGTAAGCGCTTGTTTGAACGCCGTGGTTTCCATGTTAACAAGCTTTGGCAAACCAAAATTCTCCAGGCTGCAGACACAGATATCTCAGCCTTAGTTGAAATTGAAAACAACAGTCCACACCGTTTTGAGTTCTTTATGGGGCTTTCTGGAGATCAGCCTATTTGTGCTCGTACAGCCTGGGCTTATGGAAATGCTGGTGGGCGAATTTCTCATGCTTTATCAGTATACAGCTGTCAACTTCGGCAACCAAATCAGGTGAAGACAATTTTTGAGTTTCTTGAAAATGGCTTCCACGAGATAAGCAGTTCTTTAGACTTATCTTTTGAAGATGATGCTGTGGCTGATGAGAAGATTCCTTTCCTAGCTAACCTATCCAGTGTTCTGAAAGGGAGTTCTTTTGGCACATATGAGCCACCAGCTGGAAGCAAACATTTTCGTAGTCTCATTGCTGGGTTTATGAGAACATATCACCGCATTCCACTCAAAGCTGATTAG

Protein Analysis

454

Amino Acids

51.31

Weight (kDa)

5.51

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49810
fragaria_vesca FvH4_5g27550
malus_domestica MD08G1249200.v1.1 MD15G1443800.v1.1
prunus_persica Prupe.1G586400_v2.0.a1
pyrus_communis pycom08g21570
rosa_chinensis RchiOBHm_Chr7g0218921
rosa_laevigata RLG00000002317
rosa_multiflora Rmu_sc0003823.1_g000008
rosa_roxburghii Rroxscaffold_3G00240310
rosa_rugosa Rorug07G0179800
rosa_samantha Rh7AG323100 Rh7BG314100 Rh7CG339800 Rh7DG320200
rosa_wichuraiana Rw7G027380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 16
AccB7I CCANNNNNTGG 1 cut(s) 1286
AccI GTMKAC 1 cut(s) 1092
AccII CGCG 1 cut(s) 74
AciI CCGC 3 cut(s) 72, 163, 1339
AclWI GGATC 5 cut(s) 34, 107, 120, 146, 765
AcsI RAATTY 3 cut(s) 707, 924, 1071
AcuI CTGAAG 1 cut(s) 725
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 3 cut(s) 77, 497, 1039
AfeI AGCGCT 1 cut(s) 875
AfiI CCNNNNNNNGG 2 cut(s) 175, 1286
AflII CTTAAG 1 cut(s) 85
AjnI CCWGG 4 cut(s) 252, 853, 930, 1043
AjuI GAANNNNNNNTTGG 4 cut(s) 539, 571, 615, 647
AluBI AGCT 9 cut(s) 134, 382, 806, 863, 910, 1098, 1232, 1286, 1355
AluI AGCT 9 cut(s) 134, 382, 806, 863, 910, 1098, 1232, 1286, 1355
Alw21I GWGCWC 2 cut(s) 739, 1036
Alw26I GTCTC 2 cut(s) 110, 1313
Alw44I GTGCAC 1 cut(s) 735
AlwI GGATC 5 cut(s) 34, 107, 120, 146, 765
Ama87I CYCGRG 2 cut(s) 107, 783
Aor51HI AGCGCT 1 cut(s) 875
AoxI GGCC 3 cut(s) 180, 448, 766
ApaLI GTGCAC 1 cut(s) 735
ApeKI GCWGC 2 cut(s) 80, 935
ApoI RAATTY 3 cut(s) 707, 924, 1071
ArsI GACNNNNNNTTYG 4 cut(s) 849, 881, 1172, 1204
Asp700I GAANNNNTTC 1 cut(s) 164
AspA2I CCTAGG 1 cut(s) 769
AspLEI GCGC 2 cut(s) 19, 876
AspS9I GGNCC 1 cut(s) 151
AsuHPI GGTGA 2 cut(s) 1135, 1328
AvaI CYCGRG 2 cut(s) 107, 783
AvaII GGWCC 1 cut(s) 151
AvrII CCTAGG 1 cut(s) 769
BaeGI GKGCMC 1 cut(s) 739
BamHI GGATCC 1 cut(s) 112
BanI GGYRCC 1 cut(s) 16
BauI CACGAG 1 cut(s) 1160
BbsI GAAGAC 2 cut(s) 312, 1133
Bbv12I GWGCWC 2 cut(s) 739, 1036
BbvI GCAGC 2 cut(s) 67, 922
BccI CCATC 1 cut(s) 271
BceAI ACGGC 2 cut(s) 4, 873
BciT130I CCWGG 4 cut(s) 254, 855, 932, 1045
BcoDI GTCTC 2 cut(s) 110, 1313
BfaI CTAG 4 cut(s) 465, 620, 770, 1229
BfmI CTRYAG 1 cut(s) 936
BfoI RGCGCY 2 cut(s) 20, 877
BfrI CTTAAG 1 cut(s) 85
BglII AGATCT 1 cut(s) 583
BisI GCNGC 3 cut(s) 72, 81, 936
BlnI CCTAGG 1 cut(s) 769
BlsI GCNGC 3 cut(s) 73, 82, 937
Bme1390I CCNGG 4 cut(s) 254, 855, 932, 1045
Bme18I GGWCC 1 cut(s) 151
BmeT110I CYCGRG 2 cut(s) 107, 783
BmgT120I GGNCC 1 cut(s) 151
BmiI GGNNCC 2 cut(s) 18, 114
BmrFI CCNGG 4 cut(s) 254, 855, 932, 1045
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 4 cut(s) 58, 626, 658, 1189
BmuI ACTGGG 1 cut(s) 282
BpiI GAAGAC 2 cut(s) 312, 1133
BpmI CTGGAG 4 cut(s) 119, 140, 914, 1035
BpuEI CTTGAG 2 cut(s) 114, 773
BsaBI GATNNNNATC 1 cut(s) 281
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 5 cut(s) 174, 253, 769, 889, 1044
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 1286
Bse1I ACTGG 3 cut(s) 288, 314, 1242
Bse3DI GCAATG 4 cut(s) 56, 408, 414, 1311
Bse8I GATNNNNATC 1 cut(s) 281
BseBI CCWGG 4 cut(s) 254, 855, 932, 1045
BseDI CCNNGG 5 cut(s) 174, 253, 769, 889, 1044
BseGI GGATG 1 cut(s) 641
BseJI GATNNNNATC 1 cut(s) 281
BseLI CCNNNNNNNGG 2 cut(s) 175, 1286
BseMI GCAATG 4 cut(s) 56, 408, 414, 1311
BseMII CTCAG 3 cut(s) 411, 659, 966
BseNI ACTGG 3 cut(s) 288, 314, 1242
BseSI GKGCMC 1 cut(s) 739
BseXI GCAGC 2 cut(s) 67, 922
BseYI CCCAGC 1 cut(s) 1316
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 3 cut(s) 182, 450, 768
BshNI GGYRCC 1 cut(s) 16
BsiHKAI GWGCWC 2 cut(s) 739, 1036
BsiHKCI CYCGRG 2 cut(s) 107, 783
BsiWI CGTACG 1 cut(s) 75
BslI CCNNNNNNNGG 2 cut(s) 175, 1286
BsmAI GTCTC 2 cut(s) 110, 1313
BsmBI CGTCTC 1 cut(s) 110
BsmI GAATGC 1 cut(s) 1341
BsnI GGCC 3 cut(s) 182, 450, 768
BsoBI CYCGRG 2 cut(s) 107, 783
Bsp1286I GDGCHC 2 cut(s) 739, 1036
BspACI CCGC 3 cut(s) 72, 163, 1339
BspANI GGCC 3 cut(s) 182, 450, 768
BspCNI CTCAG 3 cut(s) 412, 658, 965
BspFNI CGCG 1 cut(s) 74
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 2 cut(s) 18, 114
BspMAI CTGCAG 1 cut(s) 940
BspPI GGATC 5 cut(s) 34, 107, 120, 146, 765
BspT107I GGYRCC 1 cut(s) 16
BspTI CTTAAG 1 cut(s) 85
BsrDI GCAATG 4 cut(s) 56, 408, 414, 1311
BsrI ACTGG 3 cut(s) 288, 314, 1242
BssECI CCNNGG 5 cut(s) 174, 253, 769, 889, 1044
BssNAI GTATAC 1 cut(s) 1093
BssNI GRCGYC 1 cut(s) 17
BssSI CACGAG 1 cut(s) 1160
BssT1I CCWWGG 2 cut(s) 174, 769
Bst1107I GTATAC 1 cut(s) 1093
Bst2BI CACGAG 1 cut(s) 1160
Bst2UI CCWGG 4 cut(s) 254, 855, 932, 1045
Bst4CI ACNGT 2 cut(s) 980, 989
BstACI GRCGYC 1 cut(s) 17
BstAFI CTTAAG 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 4 cut(s) 420, 645, 952, 958
BstDSI CCRYGG 1 cut(s) 889
BstEII GGTNACC 1 cut(s) 248
BstF5I GGATG 1 cut(s) 641
BstFNI CGCG 1 cut(s) 74
BstH2I RGCGCY 2 cut(s) 20, 877
BstHHI GCGC 2 cut(s) 19, 876
BstMAI GTCTC 2 cut(s) 110, 1313
BstMWI GCNNNNNNNGC 5 cut(s) 55, 77, 80, 192, 1040
BstNI CCWGG 4 cut(s) 254, 855, 932, 1045
BstPI GGTNACC 1 cut(s) 248
BstSCI CCNGG 4 cut(s) 252, 853, 930, 1043
BstSFI CTRYAG 1 cut(s) 936
BstSLI GKGCMC 1 cut(s) 739
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 2 cut(s) 67, 922
BstV2I GAAGAC 2 cut(s) 312, 1133
BstX2I RGATCY 3 cut(s) 112, 138, 583
BstXI CCANNNNNNTGG 1 cut(s) 309
BstYI RGATCY 3 cut(s) 112, 138, 583
BstZ17I GTATAC 1 cut(s) 1093
BsuRI GGCC 3 cut(s) 182, 450, 768
BtgI CCRYGG 1 cut(s) 889
BtgZI GCGATG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 641
BtsI GCAGTG 1 cut(s) 795
BtsIMutI CAGTG 3 cut(s) 99, 795, 1249
Cac8I GCNNGC 1 cut(s) 184
CciI TCATGA 1 cut(s) 574
CfoI GCGC 2 cut(s) 19, 876
Cfr13I GGNCC 1 cut(s) 151
CseI GACGC 1 cut(s) 92
Csp6I GTAC 3 cut(s) 76, 496, 1038
CviAII CATG 5 cut(s) 483, 575, 841, 899, 1079
CviQI GTAC 3 cut(s) 76, 496, 1038
DdeI CTNAG 4 cut(s) 420, 645, 952, 958
DinI GGCGCC 1 cut(s) 18
Eco130I CCWWGG 2 cut(s) 174, 769
Eco147I AGGCCT 1 cut(s) 182
Eco32I GATATC 3 cut(s) 226, 405, 949
Eco47I GGWCC 1 cut(s) 151
Eco47III AGCGCT 1 cut(s) 875
Eco57I CTGAAG 1 cut(s) 725
Eco88I CYCGRG 2 cut(s) 107, 783
Eco91I GGTNACC 1 cut(s) 248
EcoO65I GGTNACC 1 cut(s) 248
EcoRII CCWGG 4 cut(s) 252, 853, 930, 1043
EcoRV GATATC 3 cut(s) 226, 405, 949
EcoT14I CCWWGG 2 cut(s) 174, 769
EcoT22I ATGCAT 1 cut(s) 641
EgeI GGCGCC 1 cut(s) 18
EheI GGCGCC 1 cut(s) 18
ErhI CCWWGG 2 cut(s) 174, 769
Esp3I CGTCTC 1 cut(s) 110
FaeI CATG 5 cut(s) 486, 578, 844, 902, 1082
FalI AAGNNNNNCTT 2 cut(s) 1159, 1191
FatI CATG 5 cut(s) 482, 574, 840, 898, 1078
FauNDI CATATG 1 cut(s) 1273
FblI GTMKAC 1 cut(s) 1092
Fnu4HI GCNGC 3 cut(s) 72, 81, 936
FokI GGATG 1 cut(s) 648
Fsp4HI GCNGC 3 cut(s) 72, 81, 936
FspBI CTAG 4 cut(s) 465, 620, 770, 1229
GlaI GCGC 2 cut(s) 18, 875
GluI GCNGC 3 cut(s) 72, 81, 936
GsaI CCCAGC 1 cut(s) 1320
GsuI CTGGAG 4 cut(s) 119, 140, 914, 1035
HaeII RGCGCY 2 cut(s) 20, 877
HaeIII GGCC 3 cut(s) 182, 450, 768
HgaI GACGC 1 cut(s) 92
HhaI GCGC 2 cut(s) 19, 876
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 5 cut(s) 486, 578, 844, 902, 1082
Hin6I GCGC 2 cut(s) 17, 874
HinP1I GCGC 2 cut(s) 17, 874
HincII GTYRAC 3 cut(s) 524, 904, 1103
HindII GTYRAC 3 cut(s) 524, 904, 1103
HindIII AAGCTT 1 cut(s) 908
HinfI GANTC 4 cut(s) 350, 578, 649, 1219
HpaI GTTAAC 1 cut(s) 904
HphI GGTGA 2 cut(s) 1135, 1328
Hpy166II GTNNAC 7 cut(s) 34, 524, 737, 904, 983, 1093, 1103
Hpy188I TCNGA 7 cut(s) 117, 138, 216, 349, 583, 648, 1251
Hpy188III TCNNGA 9 cut(s) 43, 107, 157, 302, 575, 665, 752, 1014, 1145
Hpy8I GTNNAC 7 cut(s) 34, 524, 737, 904, 983, 1093, 1103
HpyAV CCTTC 4 cut(s) 239, 323, 433, 791
HpyCH4III ACNGT 2 cut(s) 980, 989
HpyCH4V TGCA 4 cut(s) 377, 639, 737, 938
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 77, 80, 192, 1040
HpyF3I CTNAG 4 cut(s) 420, 645, 952, 958
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 5 cut(s) 486, 578, 844, 902, 1082
HspAI GCGC 2 cut(s) 17, 874
KasI GGCGCC 1 cut(s) 16
KspAI GTTAAC 1 cut(s) 904
LmnI GCTCC 1 cut(s) 100
Lsp1109I GCAGC 2 cut(s) 67, 922
LweI GCATC 4 cut(s) 58, 626, 658, 1189
MaeI CTAG 4 cut(s) 465, 620, 770, 1229
MaeIII GTNAC 1 cut(s) 248
MflI RGATCY 3 cut(s) 112, 138, 583
MhlI GDGCHC 2 cut(s) 739, 1036
MluCI AATT 9 cut(s) 266, 627, 707, 727, 777, 924, 966, 1071, 1131
Mly113I GGCGCC 1 cut(s) 17
MmeI TCCRAC 4 cut(s) 380, 489, 537, 613
Mph1103I ATGCAT 1 cut(s) 641
MroXI GAANNNNTTC 1 cut(s) 164
MseI TTAA 5 cut(s) 86, 500, 654, 813, 903
MspA1I CMGCKG 2 cut(s) 1098, 1286
MspCI CTTAAG 1 cut(s) 85
MspR9I CCNGG 4 cut(s) 254, 855, 932, 1045
Mva1269I GAATGC 1 cut(s) 1341
MvaI CCWGG 4 cut(s) 254, 855, 932, 1045
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 5 cut(s) 55, 77, 80, 192, 1040
NarI GGCGCC 1 cut(s) 17
NdeI CATATG 1 cut(s) 1273
NlaIII CATG 5 cut(s) 486, 578, 844, 902, 1082
NlaIV GGNNCC 2 cut(s) 18, 114
NsiI ATGCAT 1 cut(s) 641
PaeR7I CTCGAG 1 cut(s) 107
PagI TCATGA 1 cut(s) 574
PceI AGGCCT 1 cut(s) 182
PctI GAATGC 1 cut(s) 1341
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 4 cut(s) 350, 578, 649, 1219
Pfl23II CGTACG 1 cut(s) 75
PflMI CCANNNNNTGG 1 cut(s) 1286
PfoI TCCNGGA 1 cut(s) 853
PkrI GCNGC 3 cut(s) 73, 82, 937
PluTI GGCGCC 1 cut(s) 20
Psp6I CCWGG 4 cut(s) 252, 853, 930, 1043
PspEI GGTNACC 1 cut(s) 248
PspFI CCCAGC 1 cut(s) 1316
PspGI CCWGG 4 cut(s) 252, 853, 930, 1043
PspLI CGTACG 1 cut(s) 75
PspN4I GGNNCC 2 cut(s) 18, 114
PspPI GGNCC 1 cut(s) 151
PstI CTGCAG 1 cut(s) 940
PsuI RGATCY 3 cut(s) 112, 138, 583
PvuII CAGCTG 2 cut(s) 1098, 1286
RsaI GTAC 3 cut(s) 77, 497, 1039
RsaNI GTAC 3 cut(s) 76, 496, 1038
SaqAI TTAA 5 cut(s) 86, 500, 654, 813, 903
SatI GCNGC 3 cut(s) 72, 81, 936
Sau96I GGNCC 1 cut(s) 151
ScrFI CCNGG 4 cut(s) 254, 855, 932, 1045
SduI GDGCHC 2 cut(s) 739, 1036
SfaNI GCATC 4 cut(s) 58, 626, 658, 1189
SfcI CTRYAG 1 cut(s) 936
SfoI GGCGCC 1 cut(s) 18
Sfr274I CTCGAG 1 cut(s) 107
SinI GGWCC 1 cut(s) 151
SlaI CTCGAG 1 cut(s) 107
SmlI CTYRAG 4 cut(s) 85, 107, 129, 752
SmoI CTYRAG 4 cut(s) 85, 107, 129, 752
Sse9I AATT 9 cut(s) 266, 627, 707, 727, 777, 924, 966, 1071, 1131
SseBI AGGCCT 1 cut(s) 182
SsiI CCGC 3 cut(s) 72, 163, 1339
SspDI GGCGCC 1 cut(s) 16
SspMI CTAG 4 cut(s) 465, 620, 770, 1229
StuI AGGCCT 1 cut(s) 182
StyD4I CCNGG 4 cut(s) 252, 853, 930, 1043
StyI CCWWGG 2 cut(s) 174, 769
TaaI ACNGT 2 cut(s) 980, 989
TaqI TCGA 3 cut(s) 108, 219, 234
TasI AATT 9 cut(s) 266, 627, 707, 727, 777, 924, 966, 1071, 1131
TatI WGTACW 1 cut(s) 495
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 4 cut(s) 350, 578, 649, 1219
Tru1I TTAA 5 cut(s) 86, 500, 654, 813, 903
Tru9I TTAA 5 cut(s) 86, 500, 654, 813, 903
TscAI CASTG 3 cut(s) 99, 795, 1249
TseI GCWGC 2 cut(s) 80, 935
TspDTI ATGAA 4 cut(s) 258, 563, 591, 708
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 3 cut(s) 99, 795, 1249
Van91I CCANNNNNTGG 1 cut(s) 1286
Vha464I CTTAAG 1 cut(s) 85
VneI GTGCAC 1 cut(s) 735
VpaK11BI GGWCC 1 cut(s) 151
XapI RAATTY 3 cut(s) 707, 924, 1071
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XhoI CTCGAG 1 cut(s) 107
XmaJI CCTAGG 1 cut(s) 769
XmiI GTMKAC 1 cut(s) 1092
XmnI GAANNNNTTC 1 cut(s) 164
XspI CTAG 4 cut(s) 465, 620, 770, 1229
Zsp2I ATGCAT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.