Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0015103

Physical Location & Seq

Genomic Coordinates Reverse (-)

54410065 .. 54418235

Gene Structure 8171 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1443800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3237 bp

ATGGCGAAGAACGAGGCTTCGTTGGAATCGGTGGACGACTTCTTGAAGCGGTGTAGGCAGTCGGGCGATGCCGCATACGCCGCCTTGAGATCAGTGCTGGAGCGTCTCGAGGATCCAAAAACCCGAACTCAAGCTCGGATCTTCCTCACGGACCTCCAGAACCGCTTCCCCTCCAAGGAGTCCTGCGATCAATGTTTTCGCACCTACCATTTTCAGATTGAAGACATCTTCTTCGATCAGTATGAAGGTCACCAGGGTAGGAAGAAATTGACGATGATGGTTATCCCCAGTATTTTTGTTCCAGAAGACTGGTCTTTTACCTTTTTTGAAGGACTAAACAGACATTCCGATTCTATCTTCAAGGACAAGTCAGTTGCTGAGCTTGGTTGTGGAAATGGTTGGATATCCATTGCCATTGCTGAGAAGTGGTTGCCATCAAAGGTGTACGGCCTTGATATCAATCCTAGAGCAGTGAAGATGTCATGGATAAACTTGTACTTAAATGCTTTGGACGAAAAAGGTCAACCCATCTATGATGCTGAGAAGAAAAGTTTGCTGGACAGGGTAGAGTTTCATGAATCGGATCTGCTATCTTATTGTAGAGATAATGACATTCAGCTAGAACGAATTGTTGGATGCATACCTCAGATTCTTAACCCAAATCCAGATGCTATGTCTAAAATGATTACAGAGAATGCAAGTGAGGAATTTCTACATTCGTTGAGCAATTATTGTGCTCTTCAGGGTTTTCTTGAGGATCAGTTTGGCCTAGGTCTAATTGCCCGGGCAGTGGAGGAAGGAATAGCTGTCATTAAACCTATGGGGATAATGATCTTCAACATGGGGGGTCGTCCAGGACAAGCTGTTTGTAAGCACTTGTTTGAACGCCGTGGTTTCCATGTTAACAAGCTTTGGCAAACCAAAATTCTCCAGGCTGCAGATACAGATATATCAGCCTTAGTTGAAATTGAAAAGAACAGTCCACACCGTTTTGAGTTCTTTATGGGGCTTTCTGGAGATCAGCCTATTTGTGCTCGTACAGCCTGGGCCTATGGAAATGCTGGTGGCCGAATCTCTCATGCTTTATCAGTATACAGCTGTCAACTTCGCCAACCAAATCAGGTGAAGACAATTTTTGAGTTTCTTGAAAATGGCTTCCACGATATAAGCAGTTCTTTAGACTTATCATTTGAAGATGATGCTGTGGCTGATGAGAAGATTCCCTTCCTAGCTTATCTATCCAGTGTTCTGAAAGGGGGTTCTTTTGGCACATATGAGCCACCAGCTGGAAGCAAACATTTTCGTAGTCTCATTGCTGGGTTTATGAAAACATATCACCGCATTCCACTCAAAGCTGATAATGTGGTTGTCTTTCCTTCAAGGGCTGTCGCGATAGAAAATGCTCTTCGACTGTTCTCCCCTCGTCTTGCAATTGTTGATGAACATCTTACCCGACACCTTCCAAGGGAGTGGTTAACATCGTTAGCAATTGAGTGTGCAGGAACTGATAATCCTTCAGAGGATGTACTTACAGTTATTGAAGCGCCTCGCCAGTCTGACTTAATGATAGAGCTAATAAGGAAGCTGAAGCCACAGGTGGTGGTTACGGGGATTGCTGATTATGAAGCTGTTACTAATTCAGCTTTTGTGCACCTTTTAGATGTCACAAGAGAAATTGGATCTCGTTTGTTCTTGGACATATCTGATCACTTTGAGCTATCCAGCCTTCCAGGTTCCAATGGAGTCCTCAAATATATTGGAGGAACTGTGCTGCCATCCCATGCAGCAATTATATGTGGCTTGGTGAAAAATAAGGTTTATTCGGATCTAGAAGTAGCTTTTGTAATTTCTGAAGAGGAGGCCATCTTCAAGGCCTTGTCGAAGACCGTTGAACTACTAGAAGGGAATACTGCACCAATTAGCCAATGTTATTATGGTTGCCTTTTTCATGAACTTTTAGCTTTTCAGCTTGCTGACAGACATCCTCCTGCACAGAGAGAATCTGCATTGCCTAAATCAGCAGAGATGATCGGGTTTGCTAGTTCAGCAGTCTCAGTTCTCAATGATGCAGAGCTGTCAATTAGCGAGGCAGAGAACTGTTCCCTCATTCACATGGATGTCGATCAAAGCTTCTTGCGTGTACCCTCACCTGTCAAGGCTGCTATTTTTGAAAGTTTTGCTAGACAGAATATAGCTGAGTCAGAAATAGATATCACCACTAGCATCAAGCAATTCATCAAGAGTACTTATGGTTACCCAGTTGACAGCAGCAGTGAATTTATATATGCTGACAGCTCACTTGCTCTGTTTAACAAGCTTGTTCTTTGCTGCATTAAAGAAGGGGGTACATTGTGTTTTCCTGCTGGTGCAAATGGGAACTATGTTTCTGCTGCCAAATTTTTGAAAGCAAATATTGTAACTATACCAACTAAGCCCGCAGATGGCTTTAAACTGACAGACAAAGTGCTTCCTGGGGCACTAGAGACTGTGAGTAAGCCATGGGTATACATTTCTGGACCAACAATTAACCCAACTGGTTTGATATACAGCAACAAAGAGATTGAAATTTTGCTCTCTGCGTGTGCTAAAGTTGGGGCTAGAGTTGTGATAGATACTTCCTTCTCAGGCTTGGAATATGACTTTGCGGGTTGGGGTGGCTGGAATCTGGTGGACAGTTTGTCAAAGCTAAATACTAGTAACCCATCTTTTTGTGTCTCTCTGCTTGGGGGATTATCTTTGAAGATGTTGAGTGGAGCGCTCAAATTCGGGTTCCTTGTATTAAACCAGTCTGTTTTGGTGGATACATTTTATAGCTTTCCAGGATTGAGCAAGCCTCATAACACTGTTAAATATGCTGTTAAGAAGCTGCTGAGTCTTAGAGAGCAGAAACCGGGAGATCTGTGGGATGCTATTGCAGAACATATAAAAACTTTGAAGAGTCGATCCAAGTGCCTGAAAGAGACACTGGAGAAGTGCGGGTGGGATGTAGTTGAACCGTGTGGTGGTGTTTCCATGGTGGCCAAGCCCTCGTCCTACTTGAACAAGAGTGTTAAGGTTGATGACTCGAATATCCGGGAAGTCATTCACAAGGCTACTGGCTTATGCATCAACAGTGGAGCCTGGTCTGGAATTCCTGGCTACTGTCGGTTCACCATTGCACTCGAAGAAAGTGAATTCGAGCGCGCTTTGGATTGCATTGCTAAATTTAAGGATGTAACCAAGAACGGAAGTCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001887 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006732 GO:0006790 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0009987 GO:0016740 GO:0016741 GO:0017144 GO:0032259 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0046500 GO:0051186 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00450 map00450

Pfam Domains

Protein Families

Aminotran_1_2 MTS Methyltransf_31

Protein Analysis

1079

Amino Acids

119.25

Weight (kDa)

5.71

Isoelectric Point (pI)

36.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aminotran_1_2	PF00155	738 - 1065	6e-17	Aminotransferase class I and II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015103)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49810
fragaria_vesca	FvH4_5g27550
malus_domestica	MD08G1249200.v1.1 MD15G1443800.v1.1
prunus_persica	Prupe.1G586400_v2.0.a1
pyrus_communis	pycom08g21570
rosa_chinensis	RchiOBHm_Chr7g0218921
rosa_laevigata	RLG00000002317
rosa_multiflora	Rmu_sc0003823.1_g000008
rosa_roxburghii	Rroxscaffold_3G00240310
rosa_rugosa	Rorug07G0179800
rosa_samantha	Rh7AG323100 Rh7BG314100 Rh7CG339800 Rh7DG320200
rosa_wichuraiana	Rw7G027380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1286
AccI	GTMKAC	2 cut(s)	1092, 2505
AccII	CGCG	2 cut(s)	1391, 3183
AciI	CCGC	8 cut(s)	49, 72, 81, 163, 1339, 2436, 2645, 2976
AclWI	GGATC	8 cut(s)	107, 120, 146, 591, 765, 1687, 1833, 2937
AcoI	YGGCCR	2 cut(s)	1066, 3018
AcsI	RAATTY	9 cut(s)	707, 924, 2276, 2396, 2565, 2762, 3129, 3173, 3203
AcuI	CTGAAG	4 cut(s)	725, 1500, 1607, 1872
AfaI	GTAC	7 cut(s)	446, 497, 1039, 1527, 2142, 2245, 2347
AfeI	AGCGCT	1 cut(s)	2757
AfiI	CCNNNNNNNGG	6 cut(s)	175, 790, 1286, 1465, 2441, 3002
AhdI	GACNNNNNGTC	1 cut(s)	2677
AhlI	ACTAGT	1 cut(s)	2693
AjnI	CCWGG	9 cut(s)	252, 853, 930, 1043, 1729, 2470, 2818, 3119, 3133
AjuI	GAANNNNNNNTTGG	2 cut(s)	615, 647
AloI	GAACNNNNNNTCC	2 cut(s)	1495, 1527
Alw21I	GWGCWC	3 cut(s)	739, 1036, 1653
Alw26I	GTCTC	6 cut(s)	110, 1313, 2056, 2477, 2719, 2954
Alw44I	GTGCAC	1 cut(s)	1649
AlwI	GGATC	8 cut(s)	107, 120, 146, 591, 765, 1687, 1833, 2937
AlwNI	CAGNNNCTG	2 cut(s)	377, 1505
Ama87I	CYCGRG	2 cut(s)	107, 783
Aor51HI	AGCGCT	1 cut(s)	2757
AoxI	GGCC	7 cut(s)	448, 766, 1047, 1066, 1860, 1872, 3018
ApaLI	GTGCAC	1 cut(s)	1649
ApeKI	GCWGC	8 cut(s)	935, 1771, 1784, 2159, 2268, 2328, 2390, 2866
ApoI	RAATTY	9 cut(s)	707, 924, 2276, 2396, 2565, 2762, 3129, 3173, 3203
ArsI	GACNNNNNNTTYG	6 cut(s)	215, 247, 849, 881, 1172, 1204
Asp700I	GAANNNNTTC	2 cut(s)	164, 3081
AspA2I	CCTAGG	1 cut(s)	769
AspLEI	GCGC	4 cut(s)	1546, 2758, 3183, 3185
AspS9I	GGNCC	3 cut(s)	151, 1047, 2516
AsuC2I	CCSGG	4 cut(s)	784, 785, 2892, 3074
AsuHPI	GGTGA	7 cut(s)	242, 1135, 1328, 1816, 2139, 2206, 3142
AvaI	CYCGRG	2 cut(s)	107, 783
AvaII	GGWCC	2 cut(s)	151, 2516
AvrII	CCTAGG	1 cut(s)	769
BaeGI	GKGCMC	2 cut(s)	1653, 2479
BalI	TGGCCA	1 cut(s)	3020
BamHI	GGATCC	1 cut(s)	112
BbsI	GAAGAC	4 cut(s)	228, 312, 1133, 1889
Bbv12I	GWGCWC	3 cut(s)	739, 1036, 1653
BbvI	GCAGC	8 cut(s)	922, 1758, 1796, 2146, 2280, 2315, 2377, 2853
BccI	CCATC	7 cut(s)	271, 442, 536, 1783, 1871, 2435, 2710
BceAI	ACGGC	2 cut(s)	463, 873
BcgI	CGANNNNNNTGC	2 cut(s)	2931, 2965
BciT130I	CCWGG	9 cut(s)	254, 855, 932, 1045, 1731, 2472, 2820, 3121, 3135
BciVI	GTATCC	1 cut(s)	2794
BclI	TGATCA	1 cut(s)	1705
BcnI	CCSGG	4 cut(s)	784, 785, 2892, 3074
BcoDI	GTCTC	6 cut(s)	110, 1313, 2056, 2477, 2719, 2954
BcuI	ACTAGT	1 cut(s)	2693
BfmI	CTRYAG	1 cut(s)	936
BfoI	RGCGCY	2 cut(s)	1547, 2759
BfuI	GTATCC	1 cut(s)	2794
BglII	AGATCT	1 cut(s)	2896
BlnI	CCTAGG	1 cut(s)	769
BlpI	GCTNAGC	1 cut(s)	378
BmcAI	AGTACT	1 cut(s)	2245
Bme18I	GGWCC	2 cut(s)	151, 2516
BmeRI	GACNNNNNGTC	1 cut(s)	2677
BmeT110I	CYCGRG	2 cut(s)	107, 783
BmgT120I	GGNCC	3 cut(s)	151, 1047, 2516
BmiI	GGNNCC	4 cut(s)	114, 1735, 2771, 3118
BmrI	ACTGGG	2 cut(s)	282, 2252
BmsI	GCATC	9 cut(s)	58, 526, 626, 658, 1189, 2056, 2232, 2896, 3114
BmuI	ACTGGG	2 cut(s)	282, 2252
BpiI	GAAGAC	4 cut(s)	228, 312, 1133, 1889
BplI	GAGNNNNNCTC	2 cut(s)	2818, 2850
BpmI	CTGGAG	5 cut(s)	119, 140, 914, 1035, 2987
Bpu1102I	GCTNAGC	1 cut(s)	378
BpuEI	CTTGAG	3 cut(s)	106, 114, 773
BpuMI	CCSGG	4 cut(s)	784, 785, 2892, 3074
BsaBI	GATNNNNATC	5 cut(s)	281, 459, 830, 1443, 2121
BsaXI	ACNNNNNCTCC	2 cut(s)	1752, 1782
Bsc4I	CCNNNNNNNGG	6 cut(s)	175, 790, 1286, 1465, 2441, 3002
Bse1I	ACTGG	9 cut(s)	288, 314, 1242, 1552, 2258, 2539, 2785, 2970, 3100
Bse3DI	GCAATG	6 cut(s)	408, 414, 1311, 2006, 3153, 3195
Bse8I	GATNNNNATC	5 cut(s)	281, 459, 830, 1443, 2121
BseBI	CCWGG	9 cut(s)	254, 855, 932, 1045, 1731, 2472, 2820, 3121, 3135
BseGI	GGATG	8 cut(s)	641, 1528, 1775, 1981, 2122, 2911, 2989, 3217
BseJI	GATNNNNATC	5 cut(s)	281, 459, 830, 1443, 2121
BseLI	CCNNNNNNNGG	6 cut(s)	175, 790, 1286, 1465, 2441, 3002
BseMI	GCAATG	6 cut(s)	408, 414, 1311, 2006, 3153, 3195
BseMII	CTCAG	8 cut(s)	369, 411, 531, 659, 2067, 2187, 2637, 2861
BseNI	ACTGG	9 cut(s)	288, 314, 1242, 1552, 2258, 2539, 2785, 2970, 3100
BsePI	GCGCGC	1 cut(s)	3181
BseRI	GAGGAG	1 cut(s)	1871
BseSI	GKGCMC	2 cut(s)	1653, 2479
BseXI	GCAGC	8 cut(s)	922, 1758, 1796, 2146, 2280, 2315, 2377, 2853
BseYI	CCCAGC	1 cut(s)	1316
BsgI	GTGCAG	3 cut(s)	1518, 1896, 1974
Bsh1236I	CGCG	2 cut(s)	1391, 3183
BshFI	GGCC	7 cut(s)	450, 768, 1049, 1068, 1862, 1874, 3020
BsiHKAI	GWGCWC	3 cut(s)	739, 1036, 1653
BsiHKCI	CYCGRG	2 cut(s)	107, 783
BsiSI	CCGG	3 cut(s)	784, 2891, 3073
BslI	CCNNNNNNNGG	6 cut(s)	175, 790, 1286, 1465, 2441, 3002
BsmAI	GTCTC	6 cut(s)	110, 1313, 2056, 2477, 2719, 2954
BsmBI	CGTCTC	1 cut(s)	110
BsmI	GAATGC	2 cut(s)	700, 1341
BsnI	GGCC	7 cut(s)	450, 768, 1049, 1068, 1862, 1874, 3020
BsoBI	CYCGRG	2 cut(s)	107, 783
Bsp1286I	GDGCHC	4 cut(s)	739, 1036, 1653, 2479
Bsp1720I	GCTNAGC	1 cut(s)	378
Bsp19I	CCATGG	2 cut(s)	2498, 3012
Bsp68I	TCGCGA	1 cut(s)	1391
BspACI	CCGC	8 cut(s)	49, 72, 81, 163, 1339, 2436, 2645, 2976
BspANI	GGCC	7 cut(s)	450, 768, 1049, 1068, 1862, 1874, 3020
BspCNI	CTCAG	8 cut(s)	370, 412, 532, 658, 2066, 2188, 2636, 2862
BspFNI	CGCG	2 cut(s)	1391, 3183
BspHI	TCATGA	2 cut(s)	574, 1948
BspLI	GGNNCC	4 cut(s)	114, 1735, 2771, 3118
BspMAI	CTGCAG	1 cut(s)	940
BspPI	GGATC	8 cut(s)	107, 120, 146, 591, 765, 1687, 1833, 2937
BspQI	GCTCTTC	2 cut(s)	744, 1410
BsrDI	GCAATG	6 cut(s)	408, 414, 1311, 2006, 3153, 3195
BsrI	ACTGG	9 cut(s)	288, 314, 1242, 1552, 2258, 2539, 2785, 2970, 3100
BssHII	GCGCGC	1 cut(s)	3181
BssNAI	GTATAC	2 cut(s)	1093, 2506
BssT1I	CCWWGG	5 cut(s)	174, 769, 1463, 2498, 3012
Bst1107I	GTATAC	2 cut(s)	1093, 2506
Bst2UI	CCWGG	9 cut(s)	254, 855, 932, 1045, 1731, 2472, 2820, 3121, 3135
Bst6I	CTCTTC	4 cut(s)	744, 1410, 1848, 2930
BstC8I	GCNNGC	4 cut(s)	1971, 2436, 2831, 3183
BstDSI	CCRYGG	3 cut(s)	889, 2498, 3012
BstEII	GGTNACC	2 cut(s)	248, 2252
BstF5I	GGATG	8 cut(s)	641, 1528, 1775, 1981, 2122, 2911, 2989, 3217
BstFNI	CGCG	2 cut(s)	1391, 3183
BstH2I	RGCGCY	2 cut(s)	1547, 2759
BstHHI	GCGC	4 cut(s)	1546, 2758, 3183, 3185
BstMAI	GTCTC	6 cut(s)	110, 1313, 2056, 2477, 2719, 2954
BstMWI	GCNNNNNNNGC	5 cut(s)	55, 77, 80, 1040, 2045
BstNI	CCWGG	9 cut(s)	254, 855, 932, 1045, 1731, 2472, 2820, 3121, 3135
BstPI	GGTNACC	2 cut(s)	248, 2252
BstSFI	CTRYAG	1 cut(s)	936
BstSLI	GKGCMC	2 cut(s)	1653, 2479
BstUI	CGCG	2 cut(s)	1391, 3183
BstV1I	GCAGC	8 cut(s)	922, 1758, 1796, 2146, 2280, 2315, 2377, 2853
BstV2I	GAAGAC	4 cut(s)	228, 312, 1133, 1889
BstX2I	RGATCY	6 cut(s)	112, 138, 583, 1679, 1825, 2896
BstXI	CCANNNNNNTGG	2 cut(s)	309, 1470
BstYI	RGATCY	6 cut(s)	112, 138, 583, 1679, 1825, 2896
BstZ17I	GTATAC	2 cut(s)	1093, 2506
BsuI	GTATCC	1 cut(s)	2794
BsuRI	GGCC	7 cut(s)	450, 768, 1049, 1068, 1862, 1874, 3020
BtgI	CCRYGG	3 cut(s)	889, 2498, 3012
BtgZI	GCGATG	1 cut(s)	81
BtsCI	GGATG	8 cut(s)	641, 1528, 1775, 1981, 2122, 2911, 2989, 3217
BtsI	GCAGTG	3 cut(s)	477, 795, 2278
BtsIMutI	CAGTG	8 cut(s)	99, 477, 795, 1249, 2278, 2841, 2963, 3118
BtuMI	TCGCGA	1 cut(s)	1391
Cac8I	GCNNGC	4 cut(s)	1971, 2436, 2831, 3183
CaiI	CAGNNNCTG	2 cut(s)	377, 1505
CciI	TCATGA	2 cut(s)	574, 1948
CfoI	GCGC	4 cut(s)	1546, 2758, 3183, 3185
Cfr13I	GGNCC	3 cut(s)	151, 1047, 2516
Cfr9I	CCCGGG	1 cut(s)	783
CseI	GACGC	1 cut(s)	92
Csp6I	GTAC	7 cut(s)	445, 496, 1038, 1526, 2141, 2244, 2346
CviQI	GTAC	7 cut(s)	445, 496, 1038, 1526, 2141, 2244, 2346
DraI	TTTAAA	1 cut(s)	2449
DriI	GACNNNNNGTC	1 cut(s)	2677
EaeI	YGGCCR	2 cut(s)	1066, 3018
Eam1104I	CTCTTC	4 cut(s)	744, 1410, 1848, 2930
Eam1105I	GACNNNNNGTC	1 cut(s)	2677
EarI	CTCTTC	4 cut(s)	744, 1410, 1848, 2930
Eco130I	CCWWGG	5 cut(s)	174, 769, 1463, 2498, 3012
Eco147I	AGGCCT	1 cut(s)	1874
Eco32I	GATATC	3 cut(s)	405, 457, 2212
Eco47I	GGWCC	2 cut(s)	151, 2516
Eco47III	AGCGCT	1 cut(s)	2757
Eco57I	CTGAAG	4 cut(s)	725, 1500, 1607, 1872
Eco88I	CYCGRG	2 cut(s)	107, 783
Eco91I	GGTNACC	2 cut(s)	248, 2252
EcoO65I	GGTNACC	2 cut(s)	248, 2252
EcoRI	GAATTC	2 cut(s)	3129, 3173
EcoRII	CCWGG	9 cut(s)	252, 853, 930, 1043, 1729, 2470, 2818, 3119, 3133
EcoRV	GATATC	3 cut(s)	405, 457, 2212
EcoT14I	CCWWGG	5 cut(s)	174, 769, 1463, 2498, 3012
EcoT22I	ATGCAT	2 cut(s)	641, 3107
ErhI	CCWWGG	5 cut(s)	174, 769, 1463, 2498, 3012
Esp3I	CGTCTC	1 cut(s)	110
FalI	AAGNNNNNCTT	4 cut(s)	1159, 1191, 1208, 1240
FauI	CCCGC	3 cut(s)	2443, 2638, 2969
FauNDI	CATATG	1 cut(s)	1273
FbaI	TGATCA	1 cut(s)	1705
FblI	GTMKAC	2 cut(s)	1092, 2505
FokI	GGATG	8 cut(s)	648, 1535, 1762, 1968, 2129, 2918, 2996, 3224
GlaI	GCGC	4 cut(s)	1545, 2757, 3182, 3184
GsaI	CCCAGC	1 cut(s)	1320
GsuI	CTGGAG	5 cut(s)	119, 140, 914, 1035, 2987
HaeII	RGCGCY	2 cut(s)	1547, 2759
HaeIII	GGCC	7 cut(s)	450, 768, 1049, 1068, 1862, 1874, 3020
HapII	CCGG	3 cut(s)	784, 2891, 3073
HgaI	GACGC	1 cut(s)	92
HhaI	GCGC	4 cut(s)	1546, 2758, 3183, 3185
Hin6I	GCGC	4 cut(s)	1544, 2756, 3181, 3183
HinP1I	GCGC	4 cut(s)	1544, 2756, 3181, 3183
HincII	GTYRAC	5 cut(s)	524, 904, 1103, 1476, 2263
HindII	GTYRAC	5 cut(s)	524, 904, 1103, 1476, 2263
HindIII	AAGCTT	3 cut(s)	908, 2128, 2315
HpaI	GTTAAC	2 cut(s)	904, 1476
HpaII	CCGG	3 cut(s)	784, 2891, 3073
HphI	GGTGA	7 cut(s)	242, 1135, 1328, 1816, 2139, 2206, 3142
HpyF10VI	GCNNNNNNNGC	5 cut(s)	55, 77, 80, 1040, 2045
HspAI	GCGC	4 cut(s)	1544, 2756, 3181, 3183
Ksp22I	TGATCA	1 cut(s)	1705
KspAI	GTTAAC	2 cut(s)	904, 1476
LguI	GCTCTTC	2 cut(s)	744, 1410
LmnI	GCTCC	3 cut(s)	100, 2753, 3116
Lsp1109I	GCAGC	8 cut(s)	922, 1758, 1796, 2146, 2280, 2315, 2377, 2853
LweI	GCATC	9 cut(s)	58, 526, 626, 658, 1189, 2056, 2232, 2896, 3114
MaeIII	GTNAC	8 cut(s)	248, 1603, 1630, 1663, 2252, 2416, 2696, 3214
MfeI	CAATTG	2 cut(s)	1431, 1488
MflI	RGATCY	6 cut(s)	112, 138, 583, 1679, 1825, 2896
MhlI	GDGCHC	4 cut(s)	739, 1036, 1653, 2479
MlsI	TGGCCA	1 cut(s)	3020
MluNI	TGGCCA	1 cut(s)	3020
MlyI	GAGTC	6 cut(s)	188, 1752, 2207, 2881, 2947, 3056
MmeI	TCCRAC	2 cut(s)	380, 613
Mox20I	TGGCCA	1 cut(s)	3020
Mph1103I	ATGCAT	2 cut(s)	641, 3107
MroXI	GAANNNNTTC	2 cut(s)	164, 3081
MscI	TGGCCA	1 cut(s)	3020
MslI	CAYNNNNRTG	2 cut(s)	2111, 2115
Msp20I	TGGCCA	1 cut(s)	3020
MspA1I	CMGCKG	2 cut(s)	1098, 1286
MspI	CCGG	3 cut(s)	784, 2891, 3073
MunI	CAATTG	2 cut(s)	1431, 1488
Mva1269I	GAATGC	2 cut(s)	700, 1341
MvaI	CCWGG	9 cut(s)	254, 855, 932, 1045, 1731, 2472, 2820, 3121, 3135
MvnI	CGCG	2 cut(s)	1391, 3183
MwoI	GCNNNNNNNGC	5 cut(s)	55, 77, 80, 1040, 2045
NciI	CCSGG	4 cut(s)	784, 785, 2892, 3074
NcoI	CCATGG	2 cut(s)	2498, 3012
NdeI	CATATG	1 cut(s)	1273
NlaIV	GGNNCC	4 cut(s)	114, 1735, 2771, 3118
NmuCI	GTSAC	2 cut(s)	248, 1663
NruI	TCGCGA	1 cut(s)	1391
NsiI	ATGCAT	2 cut(s)	641, 3107
PaeR7I	CTCGAG	1 cut(s)	107
PagI	TCATGA	2 cut(s)	574, 1948
PauI	GCGCGC	1 cut(s)	3181
PceI	AGGCCT	1 cut(s)	1874
PciSI	GCTCTTC	2 cut(s)	744, 1410
PctI	GAATGC	2 cut(s)	700, 1341
PdmI	GAANNNNTTC	2 cut(s)	164, 3081
PfeI	GAWTC	8 cut(s)	26, 350, 578, 649, 1071, 1219, 2000, 2662
PflMI	CCANNNNNTGG	1 cut(s)	1286
PfoI	TCCNGGA	3 cut(s)	853, 2818, 3072
PleI	GAGTC	6 cut(s)	187, 1751, 2206, 2880, 2946, 3056
PpsI	GAGTC	6 cut(s)	187, 1751, 2206, 2880, 2946, 3056
Psp6I	CCWGG	9 cut(s)	252, 853, 930, 1043, 1729, 2470, 2818, 3119, 3133
PspEI	GGTNACC	2 cut(s)	248, 2252
PspFI	CCCAGC	1 cut(s)	1316
PspGI	CCWGG	9 cut(s)	252, 853, 930, 1043, 1729, 2470, 2818, 3119, 3133
PspN4I	GGNNCC	4 cut(s)	114, 1735, 2771, 3118
PspPI	GGNCC	3 cut(s)	151, 1047, 2516
PstI	CTGCAG	1 cut(s)	940
PstNI	CAGNNNCTG	2 cut(s)	377, 1505
PsuI	RGATCY	6 cut(s)	112, 138, 583, 1679, 1825, 2896
PteI	GCGCGC	1 cut(s)	3181
PvuII	CAGCTG	2 cut(s)	1098, 1286
RruI	TCGCGA	1 cut(s)	1391
RsaI	GTAC	7 cut(s)	446, 497, 1039, 1527, 2142, 2245, 2347
RsaNI	GTAC	7 cut(s)	445, 496, 1038, 1526, 2141, 2244, 2346
RseI	CAYNNNNRTG	2 cut(s)	2111, 2115
SapI	GCTCTTC	2 cut(s)	744, 1410
Sau96I	GGNCC	3 cut(s)	151, 1047, 2516
ScaI	AGTACT	1 cut(s)	2245
SchI	GAGTC	6 cut(s)	188, 1752, 2207, 2881, 2947, 3056
SduI	GDGCHC	4 cut(s)	739, 1036, 1653, 2479
SfaNI	GCATC	9 cut(s)	58, 526, 626, 658, 1189, 2056, 2232, 2896, 3114
SfcI	CTRYAG	1 cut(s)	936
Sfr274I	CTCGAG	1 cut(s)	107
SinI	GGWCC	2 cut(s)	151, 2516
SlaI	CTCGAG	1 cut(s)	107
SmaI	CCCGGG	1 cut(s)	785
SmiMI	CAYNNNNRTG	2 cut(s)	2111, 2115
SmlI	CTYRAG	4 cut(s)	85, 107, 129, 752
SmoI	CTYRAG	4 cut(s)	85, 107, 129, 752
SpeI	ACTAGT	1 cut(s)	2693
SrfI	GCCCGGGC	1 cut(s)	785
SseBI	AGGCCT	1 cut(s)	1874
SsiI	CCGC	8 cut(s)	49, 72, 81, 163, 1339, 2436, 2645, 2976
SspI	AATATT	1 cut(s)	2413
StuI	AGGCCT	1 cut(s)	1874
StyI	CCWWGG	5 cut(s)	174, 769, 1463, 2498, 3012
TaqI	TCGA	9 cut(s)	108, 234, 1408, 1880, 2121, 2941, 3065, 3162, 3177
TatI	WGTACW	3 cut(s)	495, 1525, 2243
TauI	GCSGC	2 cut(s)	74, 83
TfiI	GAWTC	8 cut(s)	26, 350, 578, 649, 1071, 1219, 2000, 2662
TscAI	CASTG	8 cut(s)	99, 477, 795, 1249, 2278, 2848, 2970, 3118
TseFI	GTSAC	2 cut(s)	248, 1663
TseI	GCWGC	8 cut(s)	935, 1771, 1784, 2159, 2268, 2328, 2390, 2866
Tsp45I	GTSAC	2 cut(s)	248, 1663
TspDTI	ATGAA	9 cut(s)	258, 563, 591, 1340, 1455, 1638, 1937, 1965, 2224
TspGWI	ACGGA	1 cut(s)	164
TspMI	CCCGGG	1 cut(s)	783
TspRI	CASTG	8 cut(s)	99, 477, 795, 1249, 2278, 2848, 2970, 3118
Van91I	CCANNNNNTGG	1 cut(s)	1286
VneI	GTGCAC	1 cut(s)	1649
VpaK11BI	GGWCC	2 cut(s)	151, 2516
XapI	RAATTY	9 cut(s)	707, 924, 2276, 2396, 2565, 2762, 3129, 3173, 3203
XbaI	TCTAGA	1 cut(s)	1828
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1931
XhoI	CTCGAG	1 cut(s)	107
XmaI	CCCGGG	1 cut(s)	783
XmaJI	CCTAGG	1 cut(s)	769
XmiI	GTMKAC	2 cut(s)	1092, 2505
XmnI	GAANNNNTTC	2 cut(s)	164, 3081
ZrmI	AGTACT	1 cut(s)	2245
Zsp2I	ATGCAT	2 cut(s)	641, 3107

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.