Rh7BG314100

Methionine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0015103
Physical Location & Seq
Forward (+)
30348051 .. 30369490
21440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG314100.1

Sequence Viewer

Length: 3123 bp
ATGTTAGGCATATTTTACGAATTGGCTGCTCAGCAAGAGGAACGAAGTCTGGATTTTGTCATCTTTCATATTTCAATAAAAAGCTACCAGGGTAGGAAGAAGCTGACAATGATGGTTATCCCTAGCATTTTTGTTCCAGAAGATTGGTCCTTCACTTTTTTCGAAGGACTAAACAGACATCCGGACTCTATCTTTAAGGACAAGTCACTTGCTGAGCTTGGCTGTGGAAATGGTTGGATATCCATTGCCATTGCTGAGAAGTGGTTGCCATCCAAGGTGTATGGACTCGATATCAATCCTAGAGCAGTCAAGATGTCATGGATAAATTTGTATCTAAATGCTTTGGACGAGGAAGGTCGGCCCATCTATGATGCTGAGAAAAAGACCTTGCTGGACAGGGTTGAGTTTCATGAATCAGATCTCCTATCTTATTGCAGAGACAATGATATTCAACTAGAACGAATTGTTGGTTGCATCCCGCAGATTCTTAACCCGAATCCAGATGCAATGTCTAAGATGATAACTGAGAATGCAAGTGAGGAATTTCTACATTCATTGAGCAATTATTGTGCGCTTCAGGGTTTTCTTGAGGATCAATTTGGCTTAGGTCTAATTGCTAGGGCAGTGGAGGAAGGAATAGCTGTCATCAAACCTATGGGGATAATGATCTTCAACATGGGAGGGCGTCCAGGACAAGCTGTTTGTAAACGCTTGTTTGGACGCCGTGGCTTTCATGTTAACAAGCTTTGGCAGACTAAAATTCTTCAGGCTGCAGACACTGATATATCAGCATTAGTTGAAATTGAAAAGAACAGTCCACACCGTTTTGAGTTTTTCATGGGACTTTCTGGAGATCAGCCTATTTGTGCTCGAACAGCATGGGCATATGAAAATGCCGGTGGTCGAATCTCGCACGCTTTATCAGTTTACAGCTGTCAACTCCGCCAACCAAATCAGGTCAAGACAATTTTTGAGTTTCTTAAGAATGGCTTCCACGACATCAGCAGTTCTTTAGATTTATCGTTTGAAGATGATTCAGTGGCTGATGAGAAGATTCCCTTCCTAGCTTATCTTTCCAGTGTTCTGAAAGGGAGTTCTTTCTGCAAGTATGAACCACCAGCTGGAAGCAAACATTTTCGCAACCTCATTGCTGGGTTTCTGAAAACGTATCACCGTGTTCCACTCACTACTGATAATGTTGTTGTCTTTCCTTCAAGGGCCGTAGCAATAGAAAACGCTCTTCGGTTGTTCTCACCTCGTCTTGCAATTGTTGATGAACATCTGACGCGCCACCTTCCAAGGAACTGGTTAACATCTTTAGCAGTTAAGTGTGCAGGAACTGATAATCCTGCAGAGGATTCACTTACAGTTATTGAAGCGCCTCGACAGTCTGATTTGATAATAGAGCTTATAAGGAAGTTGAAGCCACAGGTGGTGGTTACTGGGATAGCTGATTATGAGTCTGTTACTAGTTCAGCCTTTGTACACCTTTTAGAGGTTACAAGAGAAGTTGGATCTCGTCTTTTCTTAGACATATCTGATCATTTTGAGCTATCCAGCCTTCCAAGTTCCAATGGAGTCCTCAAATATATAGGGGGGAATGTTCTGCCATCCCATGCAGCAATTATATGTGGCCTAGTGAAAAACAAGGTTTATTCAGATCTCGAAGTAGCTTTTGTTATTTCAGAGGAGGAAAATATTTTCAAGGCCTTGTCAAAGACTGTTGAACTACTAGAAGGGAGTACAGCACCAATTAGTCAAGGTTATTATGGTTGTCTTTTTCATGAACTTTTAGCTTTTCAACTTGCTGACAGACATCCACCTCCACAGAGGGAATGCACATCAGTTAAATCAGCAGAGATGATTGGTTTTGCTAGTTCAGCAAACTCGGTCCTTAATAATGCTGAGTTGGCAATTAATGAGGCAGGGAACTCTTCCCTGATTCATATGGACGTGGATCAAAGCTTCTTACATGTACCATCACCTGTCAATGCTGCAATTTTTGAAAGCTTTGCCAGACAGAACATAGCTGAGTCAGAAATAGATGTCACCACTAGCATTAAGGAATTCATAAAGACCAATTATGGTTACCCACTTGATAGCAACACCGAATTTATATATGCTGACAGCTCGCTAGCTCTGTTTAACAAGTTGGTTCTTTGTTGCATACAAGAAGGGGGGACATTGTGCTTTCCTTCTGGCTCAAATGGAAACTATGTCTCTGCTGCAAAATTTTTGAAAGCAAATATTGTAAATATACCTACAAAGATCGAAGAAGGCTTTAAACTGACAGAAAAGGGTCTTTCTGGAGTCCTAGAGACGCTGGATAAACCATGGGTATACATTTCTGGACCAACAATTAACCCAACTGGTGCGCTATACAGCAACAAAGAGATTGAAAATTTACTGTCTACCTGTGCCAAATTTGGGGCTAGGGTTGTGATTGACACTTCATTCTCGGGTTTGGAATTTGACCCTGAGGGTTGGGGTGGCTGGAGTCTGGTGGACAGTTTGTCAAAGTTATATTCTTCTAGCAAACCATCGTTTTGTGTCTCTCTGCTCGGGGGATTGTCTTTGAAGATGCTGAGTGGGGGACTCAAATTTGGGTTTCTGGTTCTGAACCAGTCTGCTGTGGTGGAAACATTTTATAGCTTTCCAGGATTAAGCAAACCTCATAACACTGTTAAATATGCCGTTAAGAAGCTACTGGGTCTGAGAGAGCAGAAATCAGGAGATCTTTGGGATGCCATTGCAGAACAGATAAGAAATTTGAAGAGTCGATCCAAGCGCTTGAAGGAGACACTGGAGAAGTGTGGATGGGATGTGCTTGAATCTAGTGGTGGTGTTTCCATGGTGGCCAAGCCCTCATCTTATTTGAATAAGACTGTTAAGTTCAAGCAATATAAAGATGGAGGCAGCACTGAAGATGGAACAGTGCATGAAGTTAAGCTTGATGACTCTAATATCAGGGAGGTCGTTCACAAGTCTACTGGCTTATGCATCAATAGTGGCTCCTGGACTGGAATTCCTGGCTATTGTCGTTTCACCATTGCTCTTGAAGAAAGTGAGTTTGAGCGGGCATTGGATTGCATTGTTCAATTTAAGAAGACCATCAGTAACTGA

Protein Analysis

1040

Amino Acids

115.17

Weight (kDa)

5.76

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 682 - 1030 9.6e-20 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49810
fragaria_vesca FvH4_5g27550
malus_domestica MD08G1249200.v1.1 MD15G1443800.v1.1
prunus_persica Prupe.1G586400_v2.0.a1
pyrus_communis pycom08g21570
rosa_chinensis RchiOBHm_Chr7g0218921
rosa_laevigata RLG00000002317
rosa_multiflora Rmu_sc0003823.1_g000008
rosa_roxburghii Rroxscaffold_3G00240310
rosa_rugosa Rorug07G0179800
rosa_samantha Rh7AG323100 Rh7BG314100 Rh7CG339800 Rh7DG320200
rosa_wichuraiana Rw7G027380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1412
AccB7I CCANNNNNTGG 1 cut(s) 1121
AccBSI CCGCTC 1 cut(s) 3076
AccI GTMKAC 3 cut(s) 2340, 2411, 2987
AccII CGCG 1 cut(s) 1288
AccIII TCCGGA 1 cut(s) 181
AciI CCGC 3 cut(s) 479, 943, 3076
AclWI GGATC 4 cut(s) 600, 1522, 1965, 2775
AcoI YGGCCR 1 cut(s) 2856
AcuI CTGAAG 3 cut(s) 560, 749, 2943
AcyI GRCGYC 2 cut(s) 685, 721
AfaI GTAC 3 cut(s) 1485, 1744, 1977
AfeI AGCGCT 1 cut(s) 2789
AfiI CCNNNNNNNGG 3 cut(s) 1121, 1495, 2427
AflII CTTAAG 1 cut(s) 980
AflIII ACRYGT 1 cut(s) 1972
AhdI GACNNNNNGTC 1 cut(s) 2512
AhlI ACTAGT 1 cut(s) 1469
AjiI CACGTC 1 cut(s) 1954
AjnI CCWGG 5 cut(s) 87, 688, 2656, 3014, 3028
AjuI GAANNNNNNNTTGG 2 cut(s) 450, 482
AloI GAACNNNNNNTCC 2 cut(s) 1330, 1362
Alw21I GWGCWC 1 cut(s) 871
Alw26I GTCTC 5 cut(s) 432, 2224, 2312, 2557, 2792
AlwI GGATC 4 cut(s) 600, 1522, 1965, 2775
AlwNI CAGNNNCTG 4 cut(s) 779, 1043, 1340, 3120
Ama87I CYCGRG 2 cut(s) 2458, 2561
Aor13HI TCCGGA 1 cut(s) 181
Aor51HI AGCGCT 1 cut(s) 2789
AoxI GGCC 5 cut(s) 359, 1218, 1633, 1707, 2856
ApeKI GCWGC 6 cut(s) 26, 770, 1619, 1994, 2225, 2916
ArsI GACNNNNNNTTYG 2 cut(s) 684, 716
AseI ATTAAT 1 cut(s) 1917
Asp700I GAANNNNTTC 1 cut(s) 989
AspLEI GCGC 5 cut(s) 574, 1290, 1381, 2377, 2790
AspS9I GGNCC 5 cut(s) 147, 360, 1218, 1891, 2351
AsuHPI GGTGA 5 cut(s) 1163, 1245, 1974, 2041, 3037
AsuII TTCGAA 1 cut(s) 162
AsuNHI GCTAGC 1 cut(s) 2134
AvaI CYCGRG 2 cut(s) 2458, 2561
AvaII GGWCC 3 cut(s) 147, 1891, 2351
AxyI CCTNAGG 1 cut(s) 2478
BalI TGGCCA 1 cut(s) 2858
BbsI GAAGAC 1 cut(s) 3113
Bbv12I GWGCWC 1 cut(s) 871
BbvI GCAGC 6 cut(s) 13, 757, 1631, 1981, 2212, 2928
BceAI ACGGC 3 cut(s) 708, 1205, 2678
BcgI CGANNNNNNTGC 2 cut(s) 8, 42
BciT130I CCWGG 5 cut(s) 89, 690, 2658, 3016, 3030
BclI TGATCA 1 cut(s) 1540
BcoDI GTCTC 5 cut(s) 432, 2224, 2312, 2557, 2792
BcuI ACTAGT 1 cut(s) 1469
BfmI CTRYAG 2 cut(s) 771, 1350
BfoI RGCGCY 2 cut(s) 1382, 2791
BfrI CTTAAG 1 cut(s) 980
BglII AGATCT 3 cut(s) 418, 1660, 2734
BisI GCNGC 6 cut(s) 27, 771, 1620, 1995, 2226, 2917
BlpI GCTNAGC 2 cut(s) 30, 213
BlsI GCNGC 6 cut(s) 28, 772, 1621, 1996, 2227, 2918
Bme1390I CCNGG 5 cut(s) 89, 690, 2658, 3016, 3030
Bme18I GGWCC 3 cut(s) 147, 1891, 2351
BmeRI GACNNNNNGTC 1 cut(s) 2512
BmeT110I CYCGRG 2 cut(s) 2458, 2561
BmgBI CACGTC 1 cut(s) 1954
BmgT120I GGNCC 5 cut(s) 147, 360, 1218, 1891, 2351
BmiI GGNNCC 1 cut(s) 3013
BmrFI CCNGG 5 cut(s) 89, 690, 2658, 3016, 3030
BmrI ACTGGG 2 cut(s) 1452, 2717
BmsI GCATC 6 cut(s) 361, 483, 493, 2571, 2734, 3009
BmtI GCTAGC 1 cut(s) 2138
BmuI ACTGGG 2 cut(s) 1452, 2717
BpiI GAAGAC 1 cut(s) 3113
BpmI CTGGAG 4 cut(s) 870, 2328, 2515, 2825
Bpu10I CCTNAGC 1 cut(s) 604
Bpu1102I GCTNAGC 2 cut(s) 30, 213
Bpu14I TTCGAA 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 608
BsaBI GATNNNNATC 4 cut(s) 116, 294, 665, 1278
BsaHI GRCGYC 2 cut(s) 685, 721
BsaJI CCNNGG 6 cut(s) 88, 273, 724, 1298, 2333, 2850
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 3 cut(s) 1121, 1495, 2427
Bse118I RCCGGY 1 cut(s) 896
Bse1I ACTGG 9 cut(s) 1077, 1310, 1447, 2374, 2623, 2712, 2808, 2995, 3025
Bse21I CCTNAGG 1 cut(s) 2478
Bse3DI GCAATG 6 cut(s) 243, 249, 513, 1146, 2748, 3048
Bse8I GATNNNNATC 4 cut(s) 116, 294, 665, 1278
BseAI TCCGGA 1 cut(s) 181
BseBI CCWGG 5 cut(s) 89, 690, 2658, 3016, 3030
BseDI CCNNGG 6 cut(s) 88, 273, 724, 1298, 2333, 2850
BseGI GGATG 8 cut(s) 178, 269, 474, 1610, 1816, 2749, 2822, 2827
BseJI GATNNNNATC 4 cut(s) 116, 294, 665, 1278
BseLI CCNNNNNNNGG 3 cut(s) 1121, 1495, 2427
BseMI GCAATG 6 cut(s) 243, 249, 513, 1146, 2748, 3048
BseNI ACTGG 9 cut(s) 1077, 1310, 1447, 2374, 2623, 2712, 2808, 2995, 3025
BseRI GAGGAG 1 cut(s) 1703
BseXI GCAGC 6 cut(s) 13, 757, 1631, 1981, 2212, 2928
BseYI CCCAGC 1 cut(s) 1151
BsgI GTGCAG 1 cut(s) 1353
Bsh1236I CGCG 1 cut(s) 1288
BshFI GGCC 5 cut(s) 361, 1220, 1635, 1709, 2858
BsiHKAI GWGCWC 1 cut(s) 871
BsiHKCI CYCGRG 2 cut(s) 2458, 2561
BsiSI CCGG 2 cut(s) 182, 897
BslFI GGGAC 3 cut(s) 855, 2194, 2607
BslI CCNNNNNNNGG 3 cut(s) 1121, 1495, 2427
BsmAI GTCTC 5 cut(s) 432, 2224, 2312, 2557, 2792
BsmBI CGTCTC 1 cut(s) 2312
BsmFI GGGAC 3 cut(s) 855, 2194, 2607
BsmI GAATGC 2 cut(s) 535, 1841
BsnI GGCC 5 cut(s) 361, 1220, 1635, 1709, 2858
BsoBI CYCGRG 2 cut(s) 2458, 2561
Bsp119I TTCGAA 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 871
Bsp13I TCCGGA 1 cut(s) 181
Bsp1407I TGTACA 1 cut(s) 1483
Bsp1720I GCTNAGC 2 cut(s) 30, 213
Bsp19I CCATGG 2 cut(s) 2333, 2850
BspACI CCGC 3 cut(s) 479, 943, 3076
BspANI GGCC 5 cut(s) 361, 1220, 1635, 1709, 2858
BspEI TCCGGA 1 cut(s) 181
BspFNI CGCG 1 cut(s) 1288
BspHI TCATGA 2 cut(s) 409, 1783
BspLI GGNNCC 1 cut(s) 3013
BspMAI CTGCAG 2 cut(s) 775, 1354
BspOI GCTAGC 1 cut(s) 2138
BspPI GGATC 4 cut(s) 600, 1522, 1965, 2775
BspQI GCTCTTC 1 cut(s) 1245
BspT104I TTCGAA 1 cut(s) 162
BspTI CTTAAG 1 cut(s) 980
BsrBI CCGCTC 1 cut(s) 3076
BsrDI GCAATG 6 cut(s) 243, 249, 513, 1146, 2748, 3048
BsrFI RCCGGY 1 cut(s) 896
BsrGI TGTACA 1 cut(s) 1483
BsrI ACTGG 9 cut(s) 1077, 1310, 1447, 2374, 2623, 2712, 2808, 2995, 3025
BssAI RCCGGY 1 cut(s) 896
BssECI CCNNGG 6 cut(s) 88, 273, 724, 1298, 2333, 2850
BssNAI GTATAC 1 cut(s) 2341
BssNI GRCGYC 2 cut(s) 685, 721
BssT1I CCWWGG 4 cut(s) 273, 1298, 2333, 2850
Bst1107I GTATAC 1 cut(s) 2341
Bst2UI CCWGG 5 cut(s) 89, 690, 2658, 3016, 3030
Bst6I CTCTTC 3 cut(s) 1245, 1939, 2768
BstACI GRCGYC 2 cut(s) 685, 721
BstAFI CTTAAG 1 cut(s) 980
BstAUI TGTACA 1 cut(s) 1483
BstBI TTCGAA 1 cut(s) 162
BstC8I GCNNGC 4 cut(s) 915, 2132, 2136, 3078
BstDSI CCRYGG 3 cut(s) 724, 2333, 2850
BstEII GGTNACC 1 cut(s) 2087
BstENI CCTNNNNNAGG 1 cut(s) 1493
BstF5I GGATG 8 cut(s) 178, 269, 474, 1610, 1816, 2749, 2822, 2827
BstFNI CGCG 1 cut(s) 1288
BstH2I RGCGCY 2 cut(s) 1382, 2791
BstHHI GCGC 5 cut(s) 574, 1290, 1381, 2377, 2790
BstMAI GTCTC 5 cut(s) 432, 2224, 2312, 2557, 2792
BstMWI GCNNNNNNNGC 3 cut(s) 875, 1880, 1910
BstNI CCWGG 5 cut(s) 89, 690, 2658, 3016, 3030
BstNSI RCATGY 1 cut(s) 1976
BstPI GGTNACC 1 cut(s) 2087
BstSCI CCNGG 5 cut(s) 87, 688, 2656, 3014, 3028
BstSFI CTRYAG 2 cut(s) 771, 1350
BstUI CGCG 1 cut(s) 1288
BstV1I GCAGC 6 cut(s) 13, 757, 1631, 1981, 2212, 2928
BstV2I GAAGAC 1 cut(s) 3113
BstX2I RGATCY 4 cut(s) 418, 1514, 1660, 2734
BstXI CCANNNNNNTGG 2 cut(s) 144, 1305
BstYI RGATCY 4 cut(s) 418, 1514, 1660, 2734
BstZ17I GTATAC 1 cut(s) 2341
Bsu36I CCTNAGG 1 cut(s) 2478
BsuRI GGCC 5 cut(s) 361, 1220, 1635, 1709, 2858
BtgI CCRYGG 3 cut(s) 724, 2333, 2850
BtrI CACGTC 1 cut(s) 1954
BtsCI GGATG 8 cut(s) 178, 269, 474, 1610, 1816, 2749, 2822, 2827
BtsI GCAGTG 1 cut(s) 630
BtsIMutI CAGTG 8 cut(s) 630, 777, 1044, 1084, 2679, 2801, 2919, 2940
Cac8I GCNNGC 4 cut(s) 915, 2132, 2136, 3078
CaiI CAGNNNCTG 4 cut(s) 779, 1043, 1340, 3120
CciI TCATGA 2 cut(s) 409, 1783
CfoI GCGC 5 cut(s) 574, 1290, 1381, 2377, 2790
Cfr10I RCCGGY 1 cut(s) 896
Cfr13I GGNCC 5 cut(s) 147, 360, 1218, 1891, 2351
CseI GACGC 4 cut(s) 674, 729, 1294, 2329
Csp6I GTAC 3 cut(s) 1484, 1743, 1976
CviQI GTAC 3 cut(s) 1484, 1743, 1976
DraI TTTAAA 1 cut(s) 2284
DriI GACNNNNNGTC 1 cut(s) 2512
EaeI YGGCCR 1 cut(s) 2856
Eam1104I CTCTTC 3 cut(s) 1245, 1939, 2768
Eam1105I GACNNNNNGTC 1 cut(s) 2512
EarI CTCTTC 3 cut(s) 1245, 1939, 2768
EciI GGCGGA 1 cut(s) 932
Eco130I CCWWGG 4 cut(s) 273, 1298, 2333, 2850
Eco147I AGGCCT 1 cut(s) 1709
Eco32I GATATC 2 cut(s) 240, 292
Eco47I GGWCC 3 cut(s) 147, 1891, 2351
Eco47III AGCGCT 1 cut(s) 2789
Eco57I CTGAAG 3 cut(s) 560, 749, 2943
Eco81I CCTNAGG 1 cut(s) 2478
Eco88I CYCGRG 2 cut(s) 2458, 2561
Eco91I GGTNACC 1 cut(s) 2087
EcoNI CCTNNNNNAGG 1 cut(s) 1493
EcoO65I GGTNACC 1 cut(s) 2087
EcoRI GAATTC 2 cut(s) 2066, 3024
EcoRII CCWGG 5 cut(s) 87, 688, 2656, 3014, 3028
EcoRV GATATC 2 cut(s) 240, 292
EcoT14I CCWWGG 4 cut(s) 273, 1298, 2333, 2850
EcoT22I ATGCAT 1 cut(s) 3002
ErhI CCWWGG 4 cut(s) 273, 1298, 2333, 2850
Esp3I CGTCTC 1 cut(s) 2312
FalI AAGNNNNNCTT 6 cut(s) 974, 1006, 1043, 1075, 2934, 2966
FaqI GGGAC 3 cut(s) 855, 2194, 2607
FauI CCCGC 2 cut(s) 486, 3069
FauNDI CATATG 2 cut(s) 886, 1947
FbaI TGATCA 1 cut(s) 1540
FblI GTMKAC 3 cut(s) 2340, 2411, 2987
Fnu4HI GCNGC 6 cut(s) 27, 771, 1620, 1995, 2226, 2917
FokI GGATG 8 cut(s) 165, 256, 461, 1597, 1803, 2756, 2829, 2834
Fsp4HI GCNGC 6 cut(s) 27, 771, 1620, 1995, 2226, 2917
GlaI GCGC 5 cut(s) 573, 1289, 1380, 2376, 2789
GluI GCNGC 6 cut(s) 27, 771, 1620, 1995, 2226, 2917
GsaI CCCAGC 1 cut(s) 1155
GsuI CTGGAG 4 cut(s) 870, 2328, 2515, 2825
HaeII RGCGCY 2 cut(s) 1382, 2791
HaeIII GGCC 5 cut(s) 361, 1220, 1635, 1709, 2858
HapII CCGG 2 cut(s) 182, 897
HgaI GACGC 4 cut(s) 674, 729, 1294, 2329
HhaI GCGC 5 cut(s) 574, 1290, 1381, 2377, 2790
Hin1I GRCGYC 2 cut(s) 685, 721
Hin6I GCGC 5 cut(s) 572, 1288, 1379, 2375, 2788
HinP1I GCGC 5 cut(s) 572, 1288, 1379, 2375, 2788
HincII GTYRAC 3 cut(s) 739, 938, 1311
HindII GTYRAC 3 cut(s) 739, 938, 1311
HindIII AAGCTT 4 cut(s) 743, 1963, 2008, 2948
HpaI GTTAAC 2 cut(s) 739, 1311
HpaII CCGG 2 cut(s) 182, 897
HphI GGTGA 5 cut(s) 1163, 1245, 1974, 2041, 3037
HpyCH4IV ACGT 2 cut(s) 1166, 1953
HpyF10VI GCNNNNNNNGC 3 cut(s) 875, 1880, 1910
HpySE526I ACGT 2 cut(s) 1166, 1953
Hsp92I GRCGYC 2 cut(s) 685, 721
HspAI GCGC 5 cut(s) 572, 1288, 1379, 2375, 2788
Kpn2I TCCGGA 1 cut(s) 181
Ksp22I TGATCA 1 cut(s) 1540
KspAI GTTAAC 2 cut(s) 739, 1311
LguI GCTCTTC 1 cut(s) 1245
LmnI GCTCC 1 cut(s) 3017
Lsp1109I GCAGC 6 cut(s) 13, 757, 1631, 1981, 2212, 2928
LweI GCATC 6 cut(s) 361, 483, 493, 2571, 2734, 3009
MaeII ACGT 2 cut(s) 1166, 1953
MaeIII GTNAC 7 cut(s) 204, 1438, 1465, 1498, 2047, 2087, 3116
MbiI CCGCTC 1 cut(s) 3076
MfeI CAATTG 1 cut(s) 1266
MflI RGATCY 4 cut(s) 418, 1514, 1660, 2734
MhlI GDGCHC 1 cut(s) 871
MlsI TGGCCA 1 cut(s) 2858
MluNI TGGCCA 1 cut(s) 2858
MmeI TCCRAC 2 cut(s) 215, 1492
Mox20I TGGCCA 1 cut(s) 2858
Mph1103I ATGCAT 1 cut(s) 3002
MroI TCCGGA 1 cut(s) 181
MroXI GAANNNNTTC 1 cut(s) 989
MscI TGGCCA 1 cut(s) 2858
Msp20I TGGCCA 1 cut(s) 2858
MspA1I CMGCKG 2 cut(s) 933, 1121
MspCI CTTAAG 1 cut(s) 980
MspI CCGG 2 cut(s) 182, 897
MspR9I CCNGG 5 cut(s) 89, 690, 2658, 3016, 3030
MunI CAATTG 1 cut(s) 1266
Mva1269I GAATGC 2 cut(s) 535, 1841
MvaI CCWGG 5 cut(s) 89, 690, 2658, 3016, 3030
MvnI CGCG 1 cut(s) 1288
MwoI GCNNNNNNNGC 3 cut(s) 875, 1880, 1910
NcoI CCATGG 2 cut(s) 2333, 2850
NdeI CATATG 2 cut(s) 886, 1947
NheI GCTAGC 1 cut(s) 2134
NlaIV GGNNCC 1 cut(s) 3013
NmuCI GTSAC 2 cut(s) 204, 2047
NsiI ATGCAT 1 cut(s) 3002
NspI RCATGY 1 cut(s) 1976
NspV TTCGAA 1 cut(s) 162
PagI TCATGA 2 cut(s) 409, 1783
PceI AGGCCT 1 cut(s) 1709
PciI ACATGT 1 cut(s) 1972
PciSI GCTCTTC 1 cut(s) 1245
PctI GAATGC 2 cut(s) 535, 1841
PdmI GAANNNNTTC 1 cut(s) 989
PfeI GAWTC 9 cut(s) 413, 484, 496, 906, 1034, 1054, 1358, 1942, 2831
PflMI CCANNNNNTGG 1 cut(s) 1121
PfoI TCCNGGA 3 cut(s) 688, 2656, 3014
PkrI GCNGC 6 cut(s) 28, 772, 1621, 1996, 2227, 2918
PscI ACATGT 1 cut(s) 1972
PshBI ATTAAT 1 cut(s) 1917
PsiI TTATAA 1 cut(s) 1412
Psp6I CCWGG 5 cut(s) 87, 688, 2656, 3014, 3028
PspEI GGTNACC 1 cut(s) 2087
PspFI CCCAGC 1 cut(s) 1151
PspGI CCWGG 5 cut(s) 87, 688, 2656, 3014, 3028
PspN4I GGNNCC 1 cut(s) 3013
PspPI GGNCC 5 cut(s) 147, 360, 1218, 1891, 2351
PstI CTGCAG 2 cut(s) 775, 1354
PstNI CAGNNNCTG 4 cut(s) 779, 1043, 1340, 3120
PsuI RGATCY 4 cut(s) 418, 1514, 1660, 2734
PvuII CAGCTG 2 cut(s) 933, 1121
RsaI GTAC 3 cut(s) 1485, 1744, 1977
RsaNI GTAC 3 cut(s) 1484, 1743, 1976
SapI GCTCTTC 1 cut(s) 1245
SatI GCNGC 6 cut(s) 27, 771, 1620, 1995, 2226, 2917
Sau96I GGNCC 5 cut(s) 147, 360, 1218, 1891, 2351
ScrFI CCNGG 5 cut(s) 89, 690, 2658, 3016, 3030
SduI GDGCHC 1 cut(s) 871
SfaNI GCATC 6 cut(s) 361, 483, 493, 2571, 2734, 3009
SfcI CTRYAG 2 cut(s) 771, 1350
SfuI TTCGAA 1 cut(s) 162
SinI GGWCC 3 cut(s) 147, 1891, 2351
SmlI CTYRAG 2 cut(s) 587, 980
SmoI CTYRAG 2 cut(s) 587, 980
SpeI ACTAGT 1 cut(s) 1469
SseBI AGGCCT 1 cut(s) 1709
SsiI CCGC 3 cut(s) 479, 943, 3076
SspI AATATT 2 cut(s) 1699, 2248
StuI AGGCCT 1 cut(s) 1709
StyD4I CCNGG 5 cut(s) 87, 688, 2656, 3014, 3028
StyI CCWWGG 4 cut(s) 273, 1298, 2333, 2850
TaiI ACGT 2 cut(s) 1169, 1956
TaqI TCGA 8 cut(s) 162, 288, 871, 904, 1384, 1665, 2271, 2779
TaqII GACCGA 1 cut(s) 1879
TatI WGTACW 2 cut(s) 1483, 1742
TfiI GAWTC 9 cut(s) 413, 484, 496, 906, 1034, 1054, 1358, 1942, 2831
TscAI CASTG 8 cut(s) 630, 784, 1044, 1084, 2686, 2808, 2926, 2940
TseFI GTSAC 2 cut(s) 204, 2047
TseI GCWGC 6 cut(s) 26, 770, 1619, 1994, 2225, 2916
Tsp45I GTSAC 2 cut(s) 204, 2047
TspRI CASTG 8 cut(s) 630, 784, 1044, 1084, 2686, 2808, 2926, 2940
Van91I CCANNNNNTGG 1 cut(s) 1121
Vha464I CTTAAG 1 cut(s) 980
VpaK11BI GGWCC 3 cut(s) 147, 1891, 2351
VspI ATTAAT 1 cut(s) 1917
XagI CCTNNNNNAGG 1 cut(s) 1493
XceI RCATGY 1 cut(s) 1976
XmiI GTMKAC 3 cut(s) 2340, 2411, 2987
XmnI GAANNNNTTC 1 cut(s) 989
Zsp2I ATGCAT 1 cut(s) 3002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.