Rorug07G0179800

Methionine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0015103
Physical Location & Seq
Reverse (-)
14948480 .. 14952680
4201 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0179800.1

Sequence Viewer

Length: 972 bp
ATGGCCAGCAAATTAGCCTTCACCCTAGTAGCAGCTCCTACTACTCGTAATTCCTACAATCCTTCTATTCAGAGACCCTTTGTTTCAGTTTCGTCTTCCTCTACTAGGGTGCAGTCGTTTCAGTTCAGCGGGCGGCAGCTCTGCGTACGACGTCGTTTGCTGCAGGTGTTTCCCTCTAAGGCTGCCGCTGACCAACAGCAAGGAAATGAGGACGTTGTTGATACTAAGATCCTGCCATATTGTAGCATAGACAAGAAAGGGAAGAAGTCATTGGGAGAACTTGAACAAGACTTTCTTCAAGCTCTACAATCATTTTATTATGATGGAAAAGCTATTATGTCAAATGAGGAATTTGATAATCTCAAGGAAGAGCTAATGTGGGAAGGCAGCAGTGTTGTCATGCTAAGTTCTGATGAACAAAAGTTTCTGGAAGCTTCAATGGCCTACGTCTCTGGGAAACCAATTTTGAGCGACCAAGAGTATGATGATTTGAAGATAAGACTAAAGTCACAAGGGAGTGATATTGTCGTCCTGGGTCCCAGATGCAGTCTCCGTACTAGAAAGGTTTACAGTGACCTGAGTGTTGACTATTTGAAGATGCTCCTGCTGAATGTCCCAGCAACCATTGTTGCCTTAGGAGCGTTTTTCTTCTTGGATGATCTGACTGGTTTCGAAATTACATATCTTTTGGAGCTCCCAGAACCGTTCAGCTTCATTTTCACATGGTTTGCTGCCGTGCCAGTCATAGTTTATCTAGCTACACAACTCACCAAACTTATTGTGAAGGATGTCTTGATCTTAAAGGGCCCTTGTCCAAACTGTGGTACAGAGAATCAGTCATTCTTTGGGTCGATACTATCAATTTCGAATGGTGGTTCTACCAACAACCTTAAGTGCTCAAACTGTGGAACTCCGTTGGTATATGATAAGACTACGCGCTTGATTACCTTGCCAGAAGGAAGCAATGCTTAG

Protein Analysis

323

Amino Acids

35.85

Weight (kDa)

5.48

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49810
fragaria_vesca FvH4_5g27550
malus_domestica MD08G1249200.v1.1 MD15G1443800.v1.1
prunus_persica Prupe.1G586400_v2.0.a1
pyrus_communis pycom08g21570
rosa_chinensis RchiOBHm_Chr7g0218921
rosa_laevigata RLG00000002317
rosa_multiflora Rmu_sc0003823.1_g000008
rosa_roxburghii Rroxscaffold_3G00240310
rosa_rugosa Rorug07G0179800
rosa_samantha Rh7AG323100 Rh7BG314100 Rh7CG339800 Rh7DG320200
rosa_wichuraiana Rw7G027380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 154
AatII GACGTC 1 cut(s) 154
Acc36I ACCTGC 1 cut(s) 154
AccB7I CCANNNNNTGG 1 cut(s) 821
AccII CGCG 1 cut(s) 937
AciI CCGC 3 cut(s) 129, 133, 186
AclWI GGATC 1 cut(s) 223
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 350
AcyI GRCGYC 1 cut(s) 151
AfaI GTAC 3 cut(s) 147, 556, 826
AfiI CCNNNNNNNGG 2 cut(s) 105, 821
AflII CTTAAG 1 cut(s) 890
AgsI TTSAA 5 cut(s) 284, 299, 438, 493, 595
AjnI CCWGG 1 cut(s) 531
AjuI GAANNNNNNNTTGG 2 cut(s) 254, 286
AluBI AGCT 9 cut(s) 35, 139, 302, 332, 373, 434, 694, 711, 758
AluI AGCT 9 cut(s) 35, 139, 302, 332, 373, 434, 694, 711, 758
Alw21I GWGCWC 2 cut(s) 696, 899
Alw26I GTCTC 3 cut(s) 67, 454, 554
AlwI GGATC 1 cut(s) 223
AoxI GGCC 3 cut(s) 3, 441, 805
ApaI GGGCCC 1 cut(s) 809
ApeKI GCWGC 6 cut(s) 32, 136, 160, 182, 387, 731
ApoI RAATTY 1 cut(s) 350
AspLEI GCGC 1 cut(s) 939
AspS9I GGNCC 3 cut(s) 536, 805, 806
AsuHPI GGTGA 2 cut(s) 13, 760
AsuII TTCGAA 2 cut(s) 672, 866
AvaII GGWCC 1 cut(s) 536
AxyI CCTNAGG 1 cut(s) 634
BaeGI GKGCMC 1 cut(s) 809
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 2 cut(s) 696, 809
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 2 cut(s) 696, 899
BbvI GCAGC 6 cut(s) 44, 147, 148, 169, 399, 718
BccI CCATC 1 cut(s) 317
BceAI ACGGC 1 cut(s) 719
BciT130I CCWGG 1 cut(s) 533
BcoDI GTCTC 3 cut(s) 67, 454, 554
BfaI CTAG 4 cut(s) 26, 105, 558, 755
BfmI CTRYAG 1 cut(s) 161
BfrI CTTAAG 1 cut(s) 890
BfuAI ACCTGC 1 cut(s) 154
BisI GCNGC 8 cut(s) 33, 134, 137, 161, 183, 186, 388, 732
BlsI GCNGC 8 cut(s) 34, 135, 138, 162, 184, 187, 389, 733
Bme1390I CCNGG 1 cut(s) 533
Bme18I GGWCC 1 cut(s) 536
BmgT120I GGNCC 3 cut(s) 536, 805, 806
BmiI GGNNCC 3 cut(s) 537, 538, 807
BmrFI CCNGG 1 cut(s) 533
BmsI GCATC 2 cut(s) 533, 588
BoxI GACNNNNGTC 1 cut(s) 505
BpiI GAAGAC 1 cut(s) 87
Bpu14I TTCGAA 2 cut(s) 672, 866
BpuEI CTTGAG 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 151
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 532
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 821
Bse1I ACTGG 2 cut(s) 670, 740
Bse21I CCTNAGG 1 cut(s) 634
Bse3DI GCAATG 1 cut(s) 970
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 1 cut(s) 532
BseGI GGATG 2 cut(s) 661, 793
BseLI CCNNNNNNNGG 2 cut(s) 105, 821
BseMI GCAATG 1 cut(s) 970
BseMII CTCAG 1 cut(s) 569
BseNI ACTGG 2 cut(s) 670, 740
BseSI GKGCMC 1 cut(s) 809
BseXI GCAGC 6 cut(s) 44, 147, 148, 169, 399, 718
BseYI CCCAGC 1 cut(s) 616
BsgI GTGCAG 1 cut(s) 131
Bsh1236I CGCG 1 cut(s) 937
BshFI GGCC 3 cut(s) 5, 443, 807
BsiHKAI GWGCWC 2 cut(s) 696, 899
BsiWI CGTACG 1 cut(s) 145
BslFI GGGAC 2 cut(s) 522, 599
BslI CCNNNNNNNGG 2 cut(s) 105, 821
BsmAI GTCTC 3 cut(s) 67, 454, 554
BsmBI CGTCTC 1 cut(s) 454
BsmFI GGGAC 2 cut(s) 522, 599
BsnI GGCC 3 cut(s) 5, 443, 807
Bso31I GGTCTC 1 cut(s) 67
Bsp119I TTCGAA 2 cut(s) 672, 866
Bsp120I GGGCCC 1 cut(s) 805
Bsp1286I GDGCHC 3 cut(s) 696, 809, 899
Bsp143I GATC 3 cut(s) 228, 658, 795
BspACI CCGC 3 cut(s) 129, 133, 186
BspANI GGCC 3 cut(s) 5, 443, 807
BspCNI CTCAG 1 cut(s) 570
BspFNI CGCG 1 cut(s) 937
BspLI GGNNCC 3 cut(s) 537, 538, 807
BspMAI CTGCAG 1 cut(s) 165
BspMI ACCTGC 1 cut(s) 154
BspPI GGATC 1 cut(s) 223
BspQI GCTCTTC 1 cut(s) 363
BspT104I TTCGAA 2 cut(s) 672, 866
BspTI CTTAAG 1 cut(s) 890
BspTNI GGTCTC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 970
BsrI ACTGG 2 cut(s) 670, 740
BssECI CCNNGG 1 cut(s) 532
BssMI GATC 3 cut(s) 228, 658, 795
BssNI GRCGYC 1 cut(s) 151
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 4 cut(s) 572, 705, 821, 905
Bst6I CTCTTC 1 cut(s) 363
BstACI GRCGYC 1 cut(s) 151
BstAFI CTTAAG 1 cut(s) 890
BstBI TTCGAA 2 cut(s) 672, 866
BstC8I GCNNGC 2 cut(s) 7, 131
BstDEI CTNAG 6 cut(s) 177, 225, 404, 578, 634, 969
BstENI CCTNNNNNAGG 1 cut(s) 103
BstF5I GGATG 2 cut(s) 661, 793
BstFNI CGCG 1 cut(s) 937
BstHHI GCGC 1 cut(s) 939
BstKTI GATC 3 cut(s) 231, 661, 798
BstMAI GTCTC 3 cut(s) 67, 454, 554
BstMBI GATC 3 cut(s) 228, 658, 795
BstMWI GCNNNNNNNGC 2 cut(s) 440, 638
BstNI CCWGG 1 cut(s) 533
BstPAI GACNNNNGTC 1 cut(s) 505
BstSCI CCNGG 1 cut(s) 531
BstSFI CTRYAG 1 cut(s) 161
BstSLI GKGCMC 1 cut(s) 809
BstUI CGCG 1 cut(s) 937
BstV1I GCAGC 6 cut(s) 44, 147, 148, 169, 399, 718
BstV2I GAAGAC 1 cut(s) 87
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
Bsu36I CCTNAGG 1 cut(s) 634
BsuRI GGCC 3 cut(s) 5, 443, 807
BtsCI GGATG 2 cut(s) 661, 793
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 2 cut(s) 397, 577
BveI ACCTGC 1 cut(s) 154
Cac8I GCNNGC 2 cut(s) 7, 131
CfoI GCGC 1 cut(s) 939
Cfr13I GGNCC 3 cut(s) 536, 805, 806
Csp6I GTAC 3 cut(s) 146, 555, 825
CviAII CATG 2 cut(s) 400, 723
CviQI GTAC 3 cut(s) 146, 555, 825
DdeI CTNAG 6 cut(s) 177, 225, 404, 578, 634, 969
DpnI GATC 3 cut(s) 230, 660, 797
DpnII GATC 3 cut(s) 228, 658, 795
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
Ecl136II GAGCTC 1 cut(s) 694
Eco24I GRGCYC 2 cut(s) 696, 809
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 536
Eco53kI GAGCTC 1 cut(s) 694
Eco81I CCTNAGG 1 cut(s) 634
EcoICRI GAGCTC 1 cut(s) 694
EcoNI CCTNNNNNAGG 1 cut(s) 103
EcoO109I RGGNCCY 3 cut(s) 536, 805, 806
EcoRII CCWGG 1 cut(s) 531
EcoT38I GRGCYC 2 cut(s) 696, 809
Esp3I CGTCTC 1 cut(s) 454
FaeI CATG 2 cut(s) 403, 726
FalI AAGNNNNNCTT 5 cut(s) 279, 311, 776, 808, 952
FaqI GGGAC 2 cut(s) 522, 599
FatI CATG 2 cut(s) 399, 722
FauI CCCGC 1 cut(s) 122
Fnu4HI GCNGC 8 cut(s) 33, 134, 137, 161, 183, 186, 388, 732
FokI GGATG 2 cut(s) 668, 800
FriOI GRGCYC 2 cut(s) 696, 809
Fsp4HI GCNGC 8 cut(s) 33, 134, 137, 161, 183, 186, 388, 732
FspBI CTAG 4 cut(s) 26, 105, 558, 755
GlaI GCGC 1 cut(s) 938
GluI GCNGC 8 cut(s) 33, 134, 137, 161, 183, 186, 388, 732
GsaI CCCAGC 1 cut(s) 620
HaeIII GGCC 3 cut(s) 5, 443, 807
HhaI GCGC 1 cut(s) 939
Hin1I GRCGYC 1 cut(s) 151
Hin1II CATG 2 cut(s) 403, 726
Hin6I GCGC 1 cut(s) 937
HinP1I GCGC 1 cut(s) 937
HincII GTYRAC 1 cut(s) 586
HindII GTYRAC 1 cut(s) 586
HindIII AAGCTT 1 cut(s) 432
HinfI GANTC 1 cut(s) 832
HphI GGTGA 2 cut(s) 13, 760
Hpy166II GTNNAC 2 cut(s) 568, 586
Hpy188I TCNGA 3 cut(s) 72, 412, 663
Hpy188III TCNNGA 2 cut(s) 428, 793
Hpy8I GTNNAC 2 cut(s) 568, 586
Hpy99I CGWCG 2 cut(s) 153, 156
HpyAV CCTTC 5 cut(s) 28, 72, 377, 778, 950
HpyCH4III ACNGT 4 cut(s) 572, 705, 821, 905
HpyCH4IV ACGT 3 cut(s) 151, 213, 447
HpyCH4V TGCA 3 cut(s) 112, 163, 546
HpyF10VI GCNNNNNNNGC 2 cut(s) 440, 638
HpyF3I CTNAG 6 cut(s) 177, 225, 404, 578, 634, 969
HpySE526I ACGT 3 cut(s) 151, 213, 447
Hsp92I GRCGYC 1 cut(s) 151
Hsp92II CATG 2 cut(s) 403, 726
HspAI GCGC 1 cut(s) 937
KflI GGGWCCC 1 cut(s) 536
Kzo9I GATC 3 cut(s) 228, 658, 795
LguI GCTCTTC 1 cut(s) 363
LmnI GCTCC 5 cut(s) 40, 606, 638, 691, 699
Lsp1109I GCAGC 6 cut(s) 44, 147, 148, 169, 399, 718
LweI GCATC 2 cut(s) 533, 588
MaeI CTAG 4 cut(s) 26, 105, 558, 755
MaeII ACGT 3 cut(s) 151, 213, 447
MaeIII GTNAC 2 cut(s) 507, 572
MalI GATC 3 cut(s) 230, 660, 797
MboI GATC 3 cut(s) 228, 658, 795
MboII GAAGA 7 cut(s) 87, 274, 287, 380, 505, 607, 640
MflI RGATCY 1 cut(s) 228
MhlI GDGCHC 3 cut(s) 696, 809, 899
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 11, 49, 350, 462, 675, 861
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 4 cut(s) 109, 184, 202, 340
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 800, 891
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 129, 188
MspCI CTTAAG 1 cut(s) 890
MspR9I CCNGG 1 cut(s) 533
MvaI CCWGG 1 cut(s) 533
MvnI CGCG 1 cut(s) 937
MwoI GCNNNNNNNGC 2 cut(s) 440, 638
NdeII GATC 3 cut(s) 228, 658, 795
NlaIII CATG 2 cut(s) 403, 726
NlaIV GGNNCC 3 cut(s) 537, 538, 807
NmuCI GTSAC 2 cut(s) 507, 572
NspV TTCGAA 2 cut(s) 672, 866
PaqCI CACCTGC 1 cut(s) 154
PciSI GCTCTTC 1 cut(s) 363
PfeI GAWTC 1 cut(s) 832
Pfl23II CGTACG 1 cut(s) 145
PflMI CCANNNNNTGG 1 cut(s) 821
PkrI GCNGC 8 cut(s) 34, 135, 138, 162, 184, 187, 389, 733
PpuMI RGGWCCY 1 cut(s) 536
PshAI GACNNNNGTC 1 cut(s) 505
Psp124BI GAGCTC 1 cut(s) 696
Psp5II RGGWCCY 1 cut(s) 536
Psp6I CCWGG 1 cut(s) 531
PspFI CCCAGC 1 cut(s) 616
PspGI CCWGG 1 cut(s) 531
PspLI CGTACG 1 cut(s) 145
PspN4I GGNNCC 3 cut(s) 537, 538, 807
PspOMI GGGCCC 1 cut(s) 805
PspPI GGNCC 3 cut(s) 536, 805, 806
PspPPI RGGWCCY 1 cut(s) 536
PstI CTGCAG 1 cut(s) 165
PsuI RGATCY 1 cut(s) 228
RsaI GTAC 3 cut(s) 147, 556, 826
RsaNI GTAC 3 cut(s) 146, 555, 825
SacI GAGCTC 1 cut(s) 696
SapI GCTCTTC 1 cut(s) 363
SaqAI TTAA 2 cut(s) 800, 891
SatI GCNGC 8 cut(s) 33, 134, 137, 161, 183, 186, 388, 732
Sau3AI GATC 3 cut(s) 228, 658, 795
Sau96I GGNCC 3 cut(s) 536, 805, 806
ScrFI CCNGG 1 cut(s) 533
SduI GDGCHC 3 cut(s) 696, 809, 899
SfaNI GCATC 2 cut(s) 533, 588
SfcI CTRYAG 1 cut(s) 161
SfuI TTCGAA 2 cut(s) 672, 866
SinI GGWCC 1 cut(s) 536
SmlI CTYRAG 2 cut(s) 362, 890
SmoI CTYRAG 2 cut(s) 362, 890
Sse9I AATT 6 cut(s) 11, 49, 350, 462, 675, 861
SsiI CCGC 3 cut(s) 129, 133, 186
SspMI CTAG 4 cut(s) 26, 105, 558, 755
SstI GAGCTC 1 cut(s) 696
StyD4I CCNGG 1 cut(s) 531
TaaI ACNGT 4 cut(s) 572, 705, 821, 905
TaiI ACGT 3 cut(s) 154, 216, 450
TaqI TCGA 3 cut(s) 672, 851, 866
TasI AATT 6 cut(s) 11, 49, 350, 462, 675, 861
TauI GCSGC 2 cut(s) 136, 188
TfiI GAWTC 1 cut(s) 832
Tru1I TTAA 2 cut(s) 800, 891
Tru9I TTAA 2 cut(s) 800, 891
TscAI CASTG 2 cut(s) 397, 577
TseFI GTSAC 2 cut(s) 507, 572
TseI GCWGC 6 cut(s) 32, 136, 160, 182, 387, 731
Tsp45I GTSAC 2 cut(s) 507, 572
TspDTI ATGAA 2 cut(s) 429, 703
TspGWI ACGGA 2 cut(s) 542, 903
TspRI CASTG 2 cut(s) 397, 577
Van91I CCANNNNNTGG 1 cut(s) 821
Vha464I CTTAAG 1 cut(s) 890
VpaK11BI GGWCC 1 cut(s) 536
XagI CCTNNNNNAGG 1 cut(s) 103
XapI RAATTY 1 cut(s) 350
XspI CTAG 4 cut(s) 26, 105, 558, 755
ZraI GACGTC 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.